##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248492_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21825607 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.36288415712791 34.0 31.0 34.0 30.0 34.0 2 32.61521409232742 34.0 31.0 34.0 31.0 34.0 3 32.72045725921849 34.0 31.0 34.0 31.0 34.0 4 36.190313607314565 37.0 35.0 37.0 35.0 37.0 5 36.098818236761986 37.0 35.0 37.0 35.0 37.0 6 36.0877412023409 37.0 35.0 37.0 35.0 37.0 7 36.080507130912785 37.0 36.0 37.0 35.0 37.0 8 36.07063995975003 37.0 35.0 37.0 35.0 37.0 9 37.790607518956975 39.0 38.0 39.0 35.0 39.0 10-11 37.80221038067807 39.0 38.0 39.0 35.0 39.0 12-13 37.78288500750517 39.0 38.0 39.0 35.0 39.0 14-15 39.182789440861825 41.0 39.0 41.0 36.0 41.0 16-17 39.084770150951584 41.0 39.0 41.0 36.0 41.0 18-19 38.93038209658957 40.0 38.0 41.0 35.0 41.0 20-21 38.740730509808955 40.0 38.0 41.0 35.0 41.0 22-23 38.561187622410685 40.0 38.0 41.0 34.0 41.0 24-25 38.4110803882797 40.0 37.0 41.0 34.0 41.0 26-27 38.23745433517611 40.0 37.0 41.0 34.0 41.0 28-29 38.105637153642505 40.0 37.0 41.0 33.0 41.0 30-31 38.0437385086243 40.0 37.0 41.0 33.0 41.0 32-33 37.99578662348314 40.0 37.0 41.0 33.0 41.0 34-35 37.973577481716774 40.0 37.0 41.0 33.0 41.0 36-37 37.88822072623226 40.0 36.0 41.0 33.0 41.0 38-39 37.8116546311862 40.0 36.0 41.0 33.0 41.0 40-41 37.66687852026292 40.0 36.0 41.0 33.0 41.0 42-43 37.5039054125734 40.0 35.0 41.0 33.0 41.0 44-45 37.26641994424256 39.0 35.0 41.0 32.0 41.0 46-47 36.975318532950766 39.0 35.0 41.0 32.0 41.0 48-49 36.64725420924147 38.5 35.0 40.5 31.0 41.0 50-51 36.28883801490607 37.0 35.0 40.0 31.0 41.0 52-53 35.90990177272046 37.0 35.0 39.5 31.0 41.0 54-55 35.5987301292468 36.0 35.0 39.0 31.0 41.0 56-57 35.274275556230805 35.0 35.0 39.0 31.0 41.0 58-59 34.95011412511918 35.0 34.5 37.5 30.5 40.5 60-61 34.64829466140392 35.0 34.0 37.0 30.0 40.0 62-63 34.381785464202665 35.0 34.0 36.5 30.0 40.0 64-65 34.162354224558335 35.0 34.0 36.0 29.5 39.5 66-67 33.96584883068773 35.0 34.0 35.5 29.0 39.0 68-69 33.79104723181353 35.0 33.5 35.0 29.0 39.0 70-71 33.67598067261085 35.0 33.0 35.0 29.0 38.5 72-73 33.547853903902876 35.0 33.0 35.0 29.0 37.0 74-75 33.4065234703438 35.0 33.0 35.0 29.0 37.0 76-77 33.25675620384808 35.0 33.0 35.0 29.0 36.0 78-79 33.101589843526455 35.0 33.0 35.0 29.0 36.0 80-81 32.97268387541295 35.0 33.0 35.0 29.0 36.0 82-83 32.879624928644596 35.0 33.0 35.0 29.0 36.0 84-85 32.785421683804714 35.0 33.0 35.0 29.0 35.0 86-87 32.709420177867216 35.0 33.0 35.0 27.5 35.0 88-89 32.61747494582854 35.0 33.0 35.0 27.0 35.0 90-91 32.541248176969376 35.0 33.0 35.0 27.0 35.0 92-93 32.39520522842732 35.0 33.0 35.0 27.0 35.0 94-95 32.14751518709193 35.0 33.0 35.0 25.0 35.0 96-97 31.93377966532615 35.0 33.0 35.0 24.5 35.0 98-99 31.68555371678781 35.0 33.0 35.0 24.0 35.0 100 30.401438777853922 34.0 30.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28423350835823413 1101 2 0.2820470083101867 1101 3 0.2709285218562627 1101 4 0.1792318108501405 1101 5 0.1978710900470233 1101 6 0.20209456086863753 1101 7 0.2291959982325693 1101 8 0.2301242376731878 1101 9 0.3082657696074875 1101 10-11 0.3057109860689238 1101 12-13 0.3084325071555156 1101 14-15 0.4077400111898086 1101 16-17 0.43766953783521956 1101 18-19 0.4433730535519089 1101 20-21 0.42670663289517563 1101 22-23 0.44234368809706837 1101 24-25 0.41955143626872626 1101 26-27 0.4650740254036094 1101 28-29 0.47565316543957437 1101 30-31 0.46999712131417937 1101 32-33 0.485224554929907 1101 34-35 0.5290530938567386 1101 36-37 0.5249691660594138 1101 38-39 0.5236925607798284 1101 40-41 0.5026756949573041 1101 42-43 0.5420646976990326 1101 44-45 0.5014513433973846 1101 46-47 0.5054068304600889 1101 48-49 0.4819092178916691 1101 50-51 0.46828706438331125 1101 52-53 0.42385099950351446 1101 54-55 0.4422905765062666 1101 56-57 0.46182992639235465 1101 58-59 0.4449183526987568 1101 60-61 0.46532018627306826 1101 62-63 0.4567416048347823 1101 64-65 0.4400204675791741 1101 66-67 0.4364324685200742 1101 68-69 0.4613350859357723 1101 70-71 0.4426033092979509 1101 72-73 0.45089645911854603 1101 74-75 0.3960191870453258 1101 76-77 0.39491059979298626 1101 78-79 0.4473414278457497 1101 80-81 0.47832205376055725 1101 82-83 0.45966737620400977 1101 84-85 0.4302588062182551 1101 86-87 0.3948556274636701 1101 88-89 0.4369133217661769 1101 90-91 0.43858470371273484 1101 92-93 0.4379224392822536 1101 94-95 0.41889546693922597 1101 96-97 0.40857173711470907 1101 98-99 0.40234272764250534 1101 100 0.39522175330353804 1102 1 0.2246512481630134 1102 2 0.22612142030774862 1102 3 0.2351924529380085 1102 4 0.16260290922647158 1102 5 0.1787358187774828 1102 6 0.19283914724802287 1102 7 0.19187677246835477 1102 8 0.19072432273397766 1102 9 0.2735622714691175 1102 10-11 0.25755262319088956 1102 12-13 0.2602365609977966 1102 14-15 0.3541132783411811 1102 16-17 0.3578228938379624 1102 18-19 0.3801106559188341 1102 20-21 0.38706643174769795 1102 22-23 0.3560651485135793 1102 24-25 0.3543012666461465 1102 26-27 0.37221793905346345 1102 28-29 0.393819296347651 1102 30-31 0.3987338466889341 1102 32-33 0.3894129408726883 1102 34-35 0.41238811371662365 1102 36-37 0.4593429111795828 1102 38-39 0.4342180548170873 1102 40-41 0.4121656639949549 1102 42-43 0.4173457553493023 1102 44-45 0.39447358244744635 1102 46-47 0.3867028813199056 1102 48-49 0.397343059119855 1102 50-51 0.39386416534302526 1102 52-53 0.38373614487243657 1102 54-55 0.36344290629233456 1102 56-57 0.3563926120239529 1102 58-59 0.36571207317178533 1102 60-61 0.3806067090940317 1102 62-63 0.34140980924462383 1102 64-65 0.33225424538336057 1102 66-67 0.37634461763006044 1102 68-69 0.37326182206675895 1102 70-71 0.3476991919126604 1102 72-73 0.33057397656720156 1102 74-75 0.34539364453613786 1102 76-77 0.35008570537122097 1102 78-79 0.3790060158264694 1102 80-81 0.41410404073452867 1102 82-83 0.39003983007955867 1102 84-85 0.32398228975061016 1102 86-87 0.32983955290353606 1102 88-89 0.3598521779706161 1102 90-91 0.3960471636044147 1102 92-93 0.3819221507538728 1102 94-95 0.40471571963861663 1102 96-97 0.40019916051744886 1102 98-99 0.3511310959631757 1102 100 0.34509106296833636 1103 1 0.21953278602906323 1103 2 0.22588707770053418 1103 3 0.2089668897552528 1103 4 0.1309587083000281 1103 5 0.11927629106987325 1103 6 0.15182067817795541 1103 7 0.16314998919449408 1103 8 0.17938625855844492 1103 9 0.25101269114409774 1103 10-11 0.2385792666619153 1103 12-13 0.23277838433837417 1103 14-15 0.30752700905316743 1103 16-17 0.32905991212225416 1103 18-19 0.3378965608600879 1103 20-21 0.3440627068135669 1103 22-23 0.2997687710549073 1103 24-25 0.3236035128111965 1103 26-27 0.33771845332437067 1103 28-29 0.3423996691108755 1103 30-31 0.36493106003488407 1103 32-33 0.34368779874927213 1103 34-35 0.37316573942199227 1103 36-37 0.3845037647556353 1103 38-39 0.38110276100576357 1103 40-41 0.36567093634155867 1103 42-43 0.34021021269334284 1103 44-45 0.3685154263558701 1103 46-47 0.3337651474548693 1103 48-49 0.3277603707513137 1103 50-51 0.33415597112104223 1103 52-53 0.32531745499672127 1103 54-55 0.3057065582445375 1103 56-57 0.31038737373092573 1103 58-59 0.3167981318283424 1103 60-61 0.31289382024759504 1103 62-63 0.29861153227057 1103 64-65 0.2666427068818109 1103 66-67 0.2941035294493233 1103 68-69 0.2829014602874338 1103 70-71 0.289988802254328 1103 72-73 0.2795439901375758 1103 74-75 0.2595328765308267 1103 76-77 0.25300924026625893 1103 78-79 0.28066692483110245 1103 80-81 0.3092256295968454 1103 82-83 0.27664507381554415 1103 84-85 0.2757880549912315 1103 86-87 0.24791888413288632 1103 88-89 0.2463181933757994 1103 90-91 0.2600264960143477 1103 92-93 0.2659988824101944 1103 94-95 0.2823750572049377 1103 96-97 0.2880468857785168 1103 98-99 0.29783484971662233 1103 100 0.2664581493953797 1104 1 0.17439271163402026 1104 2 0.18224922619701545 1104 3 0.1706937236075916 1104 4 0.10549913291108481 1104 5 0.10957089218569394 1104 6 0.13036100924879435 1104 7 0.13947329154576238 1104 8 0.14497710990848844 1104 9 0.16073532376830002 1104 10-11 0.1731486387815906 1104 12-13 0.17963747908348182 1104 14-15 0.2316017168309088 1104 16-17 0.24461664945906136 1104 18-19 0.24751267149281375 1104 20-21 0.2505349904932501 1104 22-23 0.21755174709969793 1104 24-25 0.23104175233942925 1104 26-27 0.23078904365387842 1104 28-29 0.247024596859994 1104 30-31 0.24220050744046517 1104 32-33 0.25586662753374867 1104 34-35 0.2858824956059749 1104 36-37 0.3064463574586256 1104 38-39 0.28945637147793235 1104 40-41 0.3190219937687715 1104 42-43 0.29180331482294974 1104 44-45 0.27825392197472354 1104 46-47 0.2690613305910645 1104 48-49 0.2695898732886164 1104 50-51 0.26223303646613516 1104 52-53 0.24226613744198033 1104 54-55 0.22752374357206406 1104 56-57 0.2305881581175484 1104 58-59 0.2250559345992329 1104 60-61 0.20286366742269024 1104 62-63 0.2092212121741852 1104 64-65 0.19678319011828904 1104 66-67 0.21474104881756517 1104 68-69 0.23866708695506134 1104 70-71 0.19953473897341922 1104 72-73 0.18609119719982203 1104 74-75 0.20378057727117493 1104 76-77 0.19747337077144067 1104 78-79 0.2492221043090055 1104 80-81 0.23455350623206783 1104 82-83 0.20175512556734532 1104 84-85 0.185907406405029 1104 86-87 0.18921454592042153 1104 88-89 0.21829961812944276 1104 90-91 0.2115104899444873 1104 92-93 0.17289182522475244 1104 94-95 0.19564287861467733 1104 96-97 0.2122060390367757 1104 98-99 0.19160733303148092 1104 100 0.22956623116032304 1105 1 0.1457294382927472 1105 2 0.15653040026988663 1105 3 0.13910286375254088 1105 4 0.08837787062924463 1105 5 0.10282697338179503 1105 6 0.11385254792984512 1105 7 0.12264327250501594 1105 8 0.0948478584840231 1105 9 0.16151564606973778 1105 10-11 0.16449168443975992 1105 12-13 0.16799243476628334 1105 14-15 0.19198186711226128 1105 16-17 0.2088811738477574 1105 18-19 0.22688542646319831 1105 20-21 0.2134535502653847 1105 22-23 0.19110773303223993 1105 24-25 0.18922659917030415 1105 26-27 0.1974628978344839 1105 28-29 0.20728769137072334 1105 30-31 0.21920973900616758 1105 32-33 0.21708889727205616 1105 34-35 0.2698953887627056 1105 36-37 0.2708088104000623 1105 38-39 0.2733315829691847 1105 40-41 0.25410585090556737 1105 42-43 0.2622352976162148 1105 44-45 0.2556892735261087 1105 46-47 0.28327101386072684 1105 48-49 0.24235157506512195 1105 50-51 0.2441119893077044 1105 52-53 0.2315361603959829 1105 54-55 0.24445009418038666 1105 56-57 0.22267261172150654 1105 58-59 0.20357746928429066 1105 60-61 0.2062503552739443 1105 62-63 0.20386082844862585 1105 64-65 0.20021853111095567 1105 66-67 0.2179693925043722 1105 68-69 0.20223351861383776 1105 70-71 0.1920714635873466 1105 72-73 0.20758910766552674 1105 74-75 0.18242927778290152 1105 76-77 0.1871946921118237 1105 78-79 0.21160362735754035 1105 80-81 0.20467991788851947 1105 82-83 0.18020758829081984 1105 84-85 0.16910315665995768 1105 86-87 0.15989183902190263 1105 88-89 0.17668009512027538 1105 90-91 0.1822364476915297 1105 92-93 0.229064476393404 1105 94-95 0.21502600735807675 1105 96-97 0.23733951917755647 1105 98-99 0.24508812190023477 1105 100 0.23984903353474962 1106 1 0.10569940129197875 1106 2 0.10863619664222313 1106 3 0.10179029105601956 1106 4 0.07256285451428823 1106 5 0.09715279705091007 1106 6 0.07782565555746856 1106 7 0.09096853733361598 1106 8 0.1073581930285954 1106 9 0.12406520646015906 1106 10-11 0.1124000879892364 1106 12-13 0.13304321410706166 1106 14-15 0.16711081692506724 1106 16-17 0.16707110664182778 1106 18-19 0.17039786560107473 1106 20-21 0.1586188278167029 1106 22-23 0.1558822430989082 1106 24-25 0.1562830194339071 1106 26-27 0.17324564160254852 1106 28-29 0.18985407565684653 1106 30-31 0.19007202017135683 1106 32-33 0.19344244453586157 1106 34-35 0.21124322261715633 1106 36-37 0.25586214762970627 1106 38-39 0.236519130650926 1106 40-41 0.21121386224117344 1106 42-43 0.2116840178684214 1106 44-45 0.20177172175394276 1106 46-47 0.20722741296279423 1106 48-49 0.18436995702295178 1106 50-51 0.18346708872579143 1106 52-53 0.168082528497159 1106 54-55 0.16062367295130286 1106 56-57 0.16940999247803745 1106 58-59 0.1700066211692004 1106 60-61 0.17065872838449536 1106 62-63 0.176021658604661 1106 64-65 0.16081265131701628 1106 66-67 0.19501008277861587 1106 68-69 0.18152836138091288 1106 70-71 0.13330311915864712 1106 72-73 0.14574605956968156 1106 74-75 0.13686752347784648 1106 76-77 0.12743625655250668 1106 78-79 0.16875847148357792 1106 80-81 0.18689481340386038 1106 82-83 0.1687304474458955 1106 84-85 0.13587108788998847 1106 86-87 0.13381370590782637 1106 88-89 0.1689948517280726 1106 90-91 0.1765681891558657 1106 92-93 0.15193878086237333 1106 94-95 0.15514384748072985 1106 96-97 0.1774955154443809 1106 98-99 0.20932383193956028 1106 100 0.1794485440660054 1107 1 0.10257430467027007 1107 2 0.10478093453135529 1107 3 0.0955020842133365 1107 4 0.06008317983197031 1107 5 0.05733256773301321 1107 6 0.03670162958729151 1107 7 0.06526959918092246 1107 8 0.07522338774278126 1107 9 0.10277620627525863 1107 10-11 0.10621477361527809 1107 12-13 0.09331018034195182 1107 14-15 0.1286529580436735 1107 16-17 0.15026183519591996 1107 18-19 0.15023185716928822 1107 20-21 0.1494471487216984 1107 22-23 0.13099165749746788 1107 24-25 0.11783304476981726 1107 26-27 0.16729051433354414 1107 28-29 0.14244941237917175 1107 30-31 0.14606916494039268 1107 32-33 0.1084372766545556 1107 34-35 0.14867131346320406 1107 36-37 0.14839007578166985 1107 38-39 0.14443323760746551 1107 40-41 0.11793183387854356 1107 42-43 0.11434620050950883 1107 44-45 0.12150739438604319 1107 46-47 0.11920539624591697 1107 48-49 0.11904968009078942 1107 50-51 0.11966810241872139 1107 52-53 0.10670935794167491 1107 54-55 0.10327549831696103 1107 56-57 0.07201123263681097 1107 58-59 0.07694885816630403 1107 60-61 0.07899613713726694 1107 62-63 0.03969636950735378 1107 64-65 0.054528856317560326 1107 66-67 0.07695879021211027 1107 68-69 0.08424222626794631 1107 70-71 0.06757425897090741 1107 72-73 0.08350872902056494 1107 74-75 0.09134287413041875 1107 76-77 0.10446387298608784 1107 78-79 0.10792911739138589 1107 80-81 0.11050258363987808 1107 82-83 0.08720317774623254 1107 84-85 0.038349384607947457 1107 86-87 0.05046538512367249 1107 88-89 0.052453727204323286 1107 90-91 0.07254776241899918 1107 92-93 0.06021894035654185 1107 94-95 0.0803605129813647 1107 96-97 0.09413664422719492 1107 98-99 0.06993183977773043 1107 100 0.01665739359529539 1108 1 0.04914934605919541 1108 2 0.06640553575726926 1108 3 0.05923481944132902 1108 4 0.029823148580483405 1108 5 0.03208434481716438 1108 6 0.02774287679240217 1108 7 0.016813753556206734 1108 8 0.02547641846761195 1108 9 0.05204436307619176 1108 10-11 0.05523734836270222 1108 12-13 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2214 88-89 -0.21031965329225955 2214 90-91 -0.23815707710656397 2214 92-93 -0.24135899367480818 2214 94-95 -0.20345171442788867 2214 96-97 -0.2297915261742567 2214 98-99 -0.23449379707590268 2214 100 -0.3062892347204631 2215 1 -0.3109943942451423 2215 2 -0.3373104389365409 2215 3 -0.2988015564804698 2215 4 -0.18179787174257456 2215 5 -0.21372670477312994 2215 6 -0.23016437482309016 2215 7 -0.2699042581808442 2215 8 -0.28477043388859613 2215 9 -0.41986242572046706 2215 10-11 -0.3676261216239496 2215 12-13 -0.3407572513440087 2215 14-15 -0.45520399224596986 2215 16-17 -0.4767780190321744 2215 18-19 -0.4311015892818233 2215 20-21 -0.4554601343644151 2215 22-23 -0.4489945817081704 2215 24-25 -0.5039877056338256 2215 26-27 -0.5567326830868922 2215 28-29 -0.5406964855797938 2215 30-31 -0.56691087753223 2215 32-33 -0.539587244726647 2215 34-35 -0.5471078599941919 2215 36-37 -0.5103735210910756 2215 38-39 -0.5552873670967031 2215 40-41 -0.551005228064902 2215 42-43 -0.5972152948643412 2215 44-45 -0.5667526857051399 2215 46-47 -0.5744344006818096 2215 48-49 -0.5213999919409531 2215 50-51 -0.5156192617742121 2215 52-53 -0.46875690692410643 2215 54-55 -0.48998157557454647 2215 56-57 -0.549520149225259 2215 58-59 -0.5889680907128252 2215 60-61 -0.5666349507565371 2215 62-63 -0.4698821764740302 2215 64-65 -0.5015463807103018 2215 66-67 -0.5927512413858551 2215 68-69 -0.5761302812504638 2215 70-71 -0.48462188767904735 2215 72-73 -0.4571135650871909 2215 74-75 -0.4346669121519966 2215 76-77 -0.4024065444831635 2215 78-79 -0.3572117375151578 2215 80-81 -0.34534497153759247 2215 82-83 -0.3704010276915142 2215 84-85 -0.38114442348723543 2215 86-87 -0.3711369011917256 2215 88-89 -0.3343730021892526 2215 90-91 -0.3797596784974431 2215 92-93 -0.37772341092053097 2215 94-95 -0.40460993823353775 2215 96-97 -0.37306385728458835 2215 98-99 -0.341105929789812 2215 100 -0.46029883689631035 2216 1 -0.36817371784282216 2216 2 -0.35916862889352785 2216 3 -0.39047006046732236 2216 4 -0.2361096203459212 2216 5 -0.26815041402834083 2216 6 -0.29967657164838357 2216 7 -0.3359197526836226 2216 8 -0.3225198414063044 2216 9 -0.45832161187006903 2216 10-11 -0.4297431345549967 2216 12-13 -0.43284299662287395 2216 14-15 -0.5768737935755297 2216 16-17 -0.6203561923786012 2216 18-19 -0.6222936654673106 2216 20-21 -0.6115108824278366 2216 22-23 -0.5992331246926668 2216 24-25 -0.5807827346406711 2216 26-27 -0.6436191174028281 2216 28-29 -0.6411749561747158 2216 30-31 -0.7067952790224723 2216 32-33 -0.7023891677842045 2216 34-35 -0.7025599995806502 2216 36-37 -0.6980907995146666 2216 38-39 -0.7154973505542728 2216 40-41 -0.68750718013235 2216 42-43 -0.7220990248123087 2216 44-45 -0.8007499030631493 2216 46-47 -0.7638830046247875 2216 48-49 -0.7085755667148206 2216 50-51 -0.706361915002617 2216 52-53 -0.6861703560264232 2216 54-55 -0.6277833474906345 2216 56-57 -0.504701441625464 2216 58-59 -0.4606423341584218 2216 60-61 -0.5360215407752449 2216 62-63 -0.5118895941365196 2216 64-65 -0.5352317693818804 2216 66-67 -0.5133510469341758 2216 68-69 -0.5703045468840884 2216 70-71 -0.5192873508180966 2216 72-73 -0.4983553242951331 2216 74-75 -0.48662574436513495 2216 76-77 -0.5693944728849516 2216 78-79 -0.4944941812902002 2216 80-81 -0.4961933748564391 2216 82-83 -0.5346640757821461 2216 84-85 -0.530394476143222 2216 86-87 -0.5254616401014687 2216 88-89 -0.540469457468518 2216 90-91 -0.6538885995774351 2216 92-93 -0.7214564422949437 2216 94-95 -0.6762116908550553 2216 96-97 -0.6373653217224948 2216 98-99 -0.7014484692113996 2216 100 -0.6597740543908159 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 77.0 7 275.0 8 596.0 9 1037.0 10 1514.0 11 2182.0 12 3206.0 13 4407.0 14 6585.0 15 10099.0 16 15695.0 17 23348.0 18 31950.0 19 42115.0 20 53518.0 21 68651.0 22 85251.0 23 105946.0 24 131033.0 25 160652.0 26 194722.0 27 235508.0 28 283841.0 29 347270.0 30 413695.0 31 508709.0 32 651476.0 33 884420.0 34 1270788.0 35 2991631.0 36 3763414.0 37 6527514.0 38 2441956.0 39 559100.0 40 3426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.025815200573245 23.099923760116226 31.204383862084207 25.669877177226326 2 17.657304246778338 24.28714237413124 35.905328623554425 22.150224755535998 3 15.372337639910771 21.587078883991634 37.42367852587101 25.616904950226584 4 15.972339280185885 20.345857963996146 39.28370010511048 24.398102650707493 5 15.191103888990224 20.238269509703244 40.87176869920772 23.698857902098812 6 14.695655428964702 15.576176186073543 37.899646960563345 31.828521424398414 7 19.743340929762 17.22935815714083 34.5282447356447 28.499056177452474 8 17.359984535596194 15.943304577966606 34.57647248940201 32.12023839703519 9 18.076005858622853 17.04834142757175 36.739431805951604 28.136220907853787 10-11 21.111151685265845 26.520039053209377 35.00427273339981 17.36453652812497 12-13 19.85583722826128 32.231328090897996 29.62092646495467 18.291908215886046 14-15 21.813297105551293 27.776301937444398 30.08490439693155 20.32549656007276 16-17 26.685288237877163 27.23895956231916 32.30511724222362 13.770634957580057 18-19 26.56619126331744 21.69179303924972 32.35483439246386 19.387181304968976 20-21 22.198543893511573 22.15663400005741 37.20122735003817 18.443594756392844 22-23 22.616358390398947 24.91181344922045 37.372529891150336 15.099298269230266 24-25 23.67790763164499 26.631576379162958 33.975075831239934 15.715440157952113 26-27 26.81340775539485 26.45833859282814 30.858044406279284 15.870209245497731 28-29 24.322593837715203 29.470544827025652 33.15766123515654 13.049200100102604 30-31 23.86571756188773 28.371173426813378 33.13630660249814 14.62680240880075 32-33 24.320471522599863 29.105682284432426 32.61837262115607 13.955473571811645 34-35 24.737412272022492 28.978869869244612 32.39368899670538 13.890028862027522 36-37 25.542380104250938 28.152092173198206 31.65306696853838 14.652460754012479 38-39 26.14889595970083 28.57869135282748 30.608623044849885 14.663789642621802 40-41 26.45885005763533 28.630145362167653 30.555323603057776 14.355680977139244 42-43 26.562399701817295 27.861638735710837 31.004549943655473 14.571411618816391 44-45 26.40562081779413 26.072674316513535 31.817713331172413 15.70399153451992 46-47 25.77958869194832 25.685820208032656 32.73306780830653 15.80152329171249 48-49 24.33921467553865 26.346507662359997 32.55022676812022 16.764050893981135 50-51 24.36286223439306 26.825491173704542 31.817562861336363 16.994083730566036 52-53 24.543809813072272 27.609097683037458 31.29931908862441 16.547773415265862 54-55 24.383925185823106 27.935777790443844 31.475784212261605 16.20451281147145 56-57 23.53495302301245 27.556729305704593 31.859121269629494 17.04919640165347 58-59 23.042930718948618 27.012139914367562 32.0533330413216 17.891596325362222 60-61 22.62324024528728 26.244993176931768 32.11890271106742 19.01286386671353 62-63 22.11338039762193 26.217914580794936 32.24326132143771 19.425443700145433 64-65 21.686227867335443 25.898480330646535 32.49947779220579 19.915814009812237 66-67 20.481684656009797 25.269430078164607 34.205337336093336 20.043547929732263 68-69 19.655420327284848 23.960251641128583 36.0711573385673 20.313170693019263 70-71 19.014732382789038 22.9825881691003 37.64652773673231 20.356151711378352 72-73 19.045098282905627 22.008580603666257 38.638535054646795 20.307786058781314 74-75 19.281434664751114 21.78431993246808 38.810776241824314 20.123469160956496 76-77 19.16669597095611 22.067678590553406 39.08644383570431 19.67918160278618 78-79 18.161895614418956 22.584857401755905 40.12444623603617 19.128800747788965 80-81 17.191126303085152 22.23028463485667 41.15720149121492 19.421387570843258 82-83 17.22449731943561 21.7467266755343 41.41667515446767 19.612100850562424 84-85 17.788750400477056 21.556502687849655 41.079032092344136 19.57571481932915 86-87 18.39077592201537 22.028520176668327 40.668373658354525 18.912330242961787 88-89 18.588201172971015 22.63514017359861 40.05644354574423 18.720215107686144 90-91 18.768445402159422 22.988297986933762 39.36695372916187 18.87630288174494 92-93 18.292196783293182 23.070848166366236 39.188291996330456 19.44866305401013 94-95 17.638740221648774 23.811911096457127 39.52755963597508 19.021789045919014 96-97 16.744032609569683 25.185518129142086 40.210467162371344 17.859982098916884 98-99 16.42812581127319 26.888387410881943 39.67618807966658 17.007298698178285 100 16.387185933827155 28.521174305211062 38.825895473495734 16.265744287466052 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 230523.0 1 474965.5 2 623269.5 3 352373.5 4 114437.5 5 39689.0 6 24523.5 7 19951.5 8 19400.5 9 21514.5 10 26460.5 11 34557.5 12 47302.0 13 66078.5 14 91337.0 15 123066.0 16 159529.0 17 198789.0 18 238379.0 19 274795.0 20 305504.5 21 330322.5 22 350570.0 23 366852.5 24 378658.5 25 384553.5 26 384347.5 27 376948.0 28 362637.0 29 344694.0 30 323158.0 31 299181.0 32 274654.0 33 250137.0 34 226244.5 35 203760.0 36 182792.5 37 162900.0 38 144375.5 39 127802.5 40 114045.0 41 102521.0 42 94953.5 43 94976.0 44 108106.5 45 142598.5 46 202142.5 47 282731.5 48 375191.0 49 478970.5 50 606548.5 51 781333.0 52 1018390.0 53 1291664.5 54 1523538.0 55 1606520.5 56 1469475.0 57 1143176.0 58 748406.0 59 412544.0 60 196628.5 61 99861.0 62 50762.5 63 15788.5 64 6797.5 65 3474.5 66 2136.0 67 1549.0 68 1197.0 69 926.0 70 713.0 71 538.5 72 419.5 73 330.0 74 259.5 75 193.5 76 126.0 77 88.0 78 57.5 79 32.5 80 24.5 81 19.5 82 15.5 83 12.0 84 10.5 85 9.0 86 5.0 87 5.5 88 5.5 89 3.0 90 4.0 91 3.0 92 1.5 93 1.5 94 1.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05899950457277088 2 0.005626418545885116 3 0.0 4 0.0 5 3.207241842116923E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.0538080338384174E-4 18-19 0.0 20-21 9.690452137253273E-4 22-23 0.0 24-25 2.0617983270751644E-5 26-27 0.0 28-29 4.581774060167033E-6 30-31 2.6345200845960434E-4 32-33 6.506119165437186E-4 34-35 2.0159805864734944E-4 36-37 0.0 38-39 2.0617983270751646E-4 40-41 8.018104605292307E-5 42-43 2.84069991730356E-4 44-45 1.3058056071476042E-4 46-47 1.993071716172659E-4 48-49 5.429402261297934E-4 50-51 1.3287144774484392E-4 52-53 2.7490644361002194E-5 54-55 5.039951466183736E-5 56-57 1.2370789962450987E-4 58-59 0.0 60-61 7.1017497932589E-5 62-63 0.0 64-65 1.832709624066813E-5 66-67 0.0 68-69 6.8726610902505485E-6 70-71 9.94244971056246E-4 72-73 0.0010056994062066637 74-75 3.4821482857269446E-4 76-77 8.934459417325712E-5 78-79 9.071912639130724E-4 80-81 2.1763426785793402E-4 82-83 7.605744939877273E-4 84-85 3.413421674824439E-4 86-87 3.4821482857269446E-4 88-89 0.0011889703686133448 90-91 2.1076160676768347E-4 92-93 1.122534644740923E-4 94-95 0.0030812430554623296 96-97 5.131586947387075E-4 98-99 0.0015807120507576261 100 0.00142034995865178 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.1825607E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.8528920229152 #Duplication Level Percentage of deduplicated Percentage of total 1 95.27999525238154 82.75343139598967 2 4.206517453677881 7.306964123935862 3 0.3481075117713138 0.9070243239671881 4 0.070330418334455 0.244336009181155 5 0.028416907089626218 0.12340452815402597 6 0.01240911512556141 0.06466605216601853 7 0.008808237073092367 0.05355146043950709 8 0.006370717826812149 0.04426522140164612 9 0.005435230110181471 0.04248589084913599 >10 0.026502850257940448 0.43292444163294913 >50 0.003233711115047393 0.2007077479418487 >100 0.0026690050280434403 0.44124013620597613 >500 3.4007026797640115E-4 0.20464656352775704 >1k 4.582483008040915E-4 0.9336196666595001 >5k 1.5789804145108718E-4 0.986047806271541 >10k+ 2.473735982731107E-4 5.260684631676107 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55624 0.254856600322731 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43810 0.20072752157591767 No Hit TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42050 0.1926635992300237 No Hit TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41727 0.19118368620858975 No Hit CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38725 0.17742920047996832 No Hit TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38417 0.17601801406943687 No Hit TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35337 0.16190614996412242 No Hit TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34932 0.16005053146975476 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33024 0.15130850656295608 No Hit ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33012 0.15125352527423408 No Hit TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32711 0.1498744112821238 No Hit CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32678 0.1497232127381383 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31446 0.1440784670960125 No Hit TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31178 0.14285055164788774 No Hit CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28263 0.12949468026250083 No Hit TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28105 0.12877075996099446 No Hit CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27916 0.1279048046636229 No Hit ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26239 0.12022116956472277 No Hit GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26119 0.11967135667750271 No Hit CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26104 0.11960263006660021 No Hit GGCGGCGGGGGATGGTGGCGGAATGGTGGTGGTGAGCTGGAGATCGGAAG 25855 0.11846176832561862 No Hit TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25328 0.11604717339591059 No Hit GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24684 0.11309651090116303 No Hit GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23268 0.1066087188319665 No Hit ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23150 0.1060680694928668 No Hit ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23025 0.10549534773534593 No Hit AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22464 0.10292497248759222 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0023458683188055207 0.0 0.0 9.163548120334065E-6 0.0 2 0.006043359985360316 0.0 0.0 1.3745322180501097E-5 0.0 3 0.010382300020338495 0.0 0.0 4.123596654150329E-5 0.0 4 0.017713138516605748 0.0 0.0 5.0399514661837354E-5 0.0 5 0.0309223931320673 0.0 0.0 5.0399514661837354E-5 4.581774060167033E-6 6 0.056681126898326356 0.0 0.0 5.0399514661837354E-5 4.581774060167033E-6 7 0.08890932563754127 0.0 0.0 5.0399514661837354E-5 4.581774060167033E-6 8 0.23579183845837598 0.0 0.0 5.498128872200439E-5 4.581774060167033E-6 9 0.7738158210216101 0.0 0.0 5.498128872200439E-5 4.581774060167033E-6 10-11 1.8341276831384346 0.0 0.0 5.498128872200439E-5 4.581774060167033E-6 12-13 3.0206055666630487 0.0 0.0 5.727217575208791E-5 4.581774060167033E-6 14-15 4.345322446243992 0.0 0.0 6.414483684233845E-5 4.581774060167033E-6 16-17 5.676387373785298 0.0 0.0 6.872661090250548E-5 4.581774060167033E-6 18-19 7.106272004256285 0.0 0.0 7.559927199275604E-5 4.581774060167033E-6 20-21 8.97276304846871 0.0 0.0 7.789015902283955E-5 4.581774060167033E-6 22-23 11.228198601761683 0.0 0.0 7.789015902283955E-5 4.581774060167033E-6 24-25 13.895175973799951 0.0 0.0 8.018104605292306E-5 4.581774060167033E-6 26-27 17.143452184399727 0.0 0.0 8.247193308300658E-5 4.581774060167033E-6 28-29 21.474596330814535 0.0 0.0 8.247193308300658E-5 4.581774060167033E-6 30-31 26.80584553730854 0.0 0.0 8.247193308300658E-5 4.581774060167033E-6 32-33 32.38219903803821 0.0 0.0 8.247193308300658E-5 4.581774060167033E-6 34-35 36.926361314945325 0.0 0.0 8.247193308300658E-5 4.581774060167033E-6 36-37 39.89164654160592 0.0 0.0 8.934459417325714E-5 4.581774060167033E-6 38-39 41.77438226574867 0.0 0.0 1.0996257744400878E-4 4.581774060167033E-6 40-41 43.298924057415675 6.8726610902505485E-6 0.0 1.1683523853425932E-4 4.581774060167033E-6 42-43 44.48411217154236 9.163548120334065E-6 0.0 1.1912612556434284E-4 4.581774060167033E-6 44-45 45.31591721595647 9.163548120334065E-6 0.0 1.1912612556434284E-4 4.581774060167033E-6 46-47 45.97700535888876 9.163548120334065E-6 0.0 1.2370789962450987E-4 4.581774060167033E-6 48-49 46.53649266203685 9.163548120334065E-6 0.0 1.282896736846769E-4 4.581774060167033E-6 50-51 47.06746529432148 9.163548120334065E-6 0.0 1.580712050757626E-4 4.581774060167033E-6 52-53 47.61838697086408 9.163548120334065E-6 0.0 1.9243451052701536E-4 4.581774060167033E-6 54-55 48.137334737127816 9.163548120334065E-6 0.0 2.2908870300835163E-4 4.581774060167033E-6 56-57 48.65115091644415 9.163548120334065E-6 0.0 2.4741579924901974E-4 4.581774060167033E-6 58-59 49.13418902851133 1.3745322180501097E-5 0.0 2.4741579924901974E-4 4.581774060167033E-6 60-61 49.59765151090643 1.3745322180501097E-5 0.0 2.9323353985069003E-4 4.581774060167033E-6 62-63 50.04346958139583 1.3745322180501097E-5 0.0 3.161424101515252E-4 4.581774060167033E-6 64-65 50.46993194736807 1.3745322180501097E-5 0.0 3.390512804523604E-4 4.581774060167033E-6 66-67 50.855710450573035 1.3745322180501097E-5 0.0 3.5737837669302854E-4 4.581774060167033E-6 68-69 51.19157052539249 1.3745322180501097E-5 0.0 3.711236988735296E-4 4.581774060167033E-6 70-71 51.50491576248028 1.3745322180501097E-5 0.0 3.7570547293369665E-4 4.581774060167033E-6 72-73 51.807498412300745 1.3745322180501097E-5 0.0 3.7570547293369665E-4 4.581774060167033E-6 74-75 52.098175322225856 1.3745322180501097E-5 0.0 3.963234562044483E-4 6.8726610902505485E-6 76-77 52.38345948408217 1.3745322180501097E-5 0.0 4.2152321353536694E-4 9.163548120334065E-6 78-79 52.65196060755607 1.3745322180501097E-5 0.0 4.994133725582065E-4 9.163548120334065E-6 80-81 52.91377463178917 1.3745322180501097E-5 0.0 5.818853056412132E-4 9.163548120334065E-6 82-83 53.17207901709217 1.3745322180501097E-5 0.0 5.956306278217142E-4 9.163548120334065E-6 84-85 53.431363902044055 1.3745322180501097E-5 0.0 6.093759500022152E-4 9.163548120334065E-6 86-87 53.69006231991624 1.3745322180501097E-5 0.0 6.139577240623824E-4 9.163548120334065E-6 88 53.88835233769214 1.3745322180501097E-5 0.0 6.139577240623824E-4 9.163548120334065E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCCTA 14140 0.0 61.85627 6 CGGCGGG 5650 0.0 61.556374 3 TGCTCTA 11995 0.0 58.106895 6 GCCCTAC 34655 0.0 57.163452 7 CCCCTAC 92165 0.0 57.144096 7 CCCCCTA 51500 0.0 54.326965 6 GCCCCTA 10095 0.0 54.14522 6 GCGGCGG 6670 0.0 52.42805 2 GGCGGCG 6830 0.0 51.848564 1 CTCCCTA 35230 0.0 51.734703 6 GCCTTTT 2210 0.0 51.07183 1 CCGCCTA 5825 0.0 50.912003 6 CGCCCTA 14580 0.0 50.41572 6 TCCCCTA 30170 0.0 50.363644 6 CCCGGAA 10340 0.0 48.771484 6 TTGCCCT 6500 0.0 48.734474 5 TTGCTCT 4855 0.0 48.69322 5 ATGCCCT 4740 0.0 48.288128 5 GTTACAC 2375 0.0 48.28508 7 CCGGAAG 34345 0.0 47.525642 7 >>END_MODULE