##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248491_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16089156
Sequences flagged as poor quality	0
Sequence length	100
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.725404614138863	33.0	31.0	34.0	26.0	34.0
2	31.02628068246712	34.0	31.0	34.0	27.0	34.0
3	31.23381891505061	34.0	31.0	34.0	27.0	34.0
4	34.630794865808994	37.0	35.0	37.0	31.0	37.0
5	34.574273690925736	37.0	35.0	37.0	31.0	37.0
6	34.51238038838085	37.0	35.0	37.0	30.0	37.0
7	34.56241725793448	37.0	35.0	37.0	31.0	37.0
8	34.51872310766332	37.0	35.0	37.0	30.0	37.0
9	36.03064958783419	39.0	37.0	39.0	30.0	39.0
10-11	36.27507577153208	39.0	37.0	39.0	31.0	39.0
12-13	36.29448869163802	39.0	37.0	39.0	31.0	39.0
14-15	37.4678966068823	40.0	38.0	41.0	31.0	41.0
16-17	37.3048909464238	40.0	37.5	41.0	31.0	41.0
18-19	36.95587298053422	40.0	36.5	41.0	30.0	41.0
20-21	36.732972133528946	39.5	35.0	41.0	30.0	41.0
22-23	36.58264532334698	39.0	35.0	41.0	30.0	41.0
24-25	36.444359138540264	39.0	35.0	41.0	28.5	41.0
26-27	36.287412932039445	39.0	35.0	41.0	27.5	41.0
28-29	36.14710460884337	39.0	35.0	41.0	26.5	41.0
30-31	36.08361237220896	39.0	35.0	41.0	26.0	41.0
32-33	35.99452357848976	39.0	35.0	41.0	25.0	41.0
34-35	35.88206662300993	39.0	35.0	41.0	25.0	41.0
36-37	35.77768933311356	39.0	35.0	40.5	25.0	41.0
38-39	35.61308309149342	38.5	35.0	40.0	25.0	41.0
40-41	35.42204591092286	38.0	34.5	40.0	25.0	41.0
42-43	35.170917852993654	38.0	34.0	40.0	25.0	41.0
44-45	34.966621369076165	38.0	34.0	40.0	25.0	41.0
46-47	34.79878006030894	37.5	33.0	40.0	25.0	41.0
48-49	34.69786587935377	37.0	33.0	40.0	25.0	41.0
50-51	34.42754094745554	36.0	33.0	39.0	25.0	41.0
52-53	34.075819017479844	35.5	33.0	39.0	25.0	41.0
54-55	33.89623591815506	35.0	33.0	39.0	25.5	41.0
56-57	33.75970255991054	35.0	33.0	38.0	26.0	40.0
58-59	33.48261826164156	35.0	33.0	37.0	25.5	40.0
60-61	33.221789166566595	35.0	32.5	36.5	25.0	40.0
62-63	32.97479591222809	35.0	32.0	36.0	25.0	39.0
64-65	32.808390290951245	35.0	32.0	36.0	25.0	39.0
66-67	32.65593363007979	35.0	32.0	35.0	25.0	39.0
68-69	32.48688386761866	35.0	32.0	35.0	25.0	38.0
70-71	32.31253140313886	35.0	32.0	35.0	25.0	37.0
72-73	32.14848628479953	35.0	32.0	35.0	24.5	37.0
74-75	31.982003157903375	35.0	31.0	35.0	24.0	36.0
76-77	31.80466293570651	35.0	31.0	35.0	24.0	36.0
78-79	31.66335654897	35.0	31.0	35.0	23.5	36.0
80-81	31.50984700502624	35.0	31.0	35.0	23.0	36.0
82-83	31.38120834306038	35.0	31.0	35.0	23.0	36.0
84-85	31.26538943372791	35.0	31.0	35.0	22.0	36.0
86-87	31.09449526128033	35.0	31.0	35.0	20.0	36.0
88-89	30.93224302753979	35.0	30.5	35.0	20.0	35.5
90-91	30.680829559984375	35.0	30.0	35.0	18.5	35.0
92-93	30.34239226097379	35.0	29.5	35.0	16.0	35.0
94-95	29.87552877229856	34.5	29.0	35.0	11.0	35.0
96-97	29.263436223752194	34.0	27.5	35.0	9.5	35.0
98-99	28.453954794148306	34.0	25.0	35.0	8.0	35.0
100	26.904053326352233	33.0	23.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.524465747588927
1101	2	0.44938597398639146
1101	3	0.4066890559946863
1101	4	0.354936527192919
1101	5	0.3936715168204685
1101	6	0.40534828155215763
1101	7	0.3288932590450315
1101	8	0.32794581224258224
1101	9	0.35723902963970033
1101	10-11	0.42582130700367316
1101	12-13	0.4435787945041554
1101	14-15	0.5771817646304029
1101	16-17	0.5855977235467549
1101	18-19	0.574730868702801
1101	20-21	0.5970464622685228
1101	22-23	0.5929776107819293
1101	24-25	0.6138066497481347
1101	26-27	0.6194479582997445
1101	28-29	0.5738577910441833
1101	30-31	0.59269607717264
1101	32-33	0.5742200953785854
1101	34-35	0.6028600027120277
1101	36-37	0.5545756093928276
1101	38-39	0.5015547654272723
1101	40-41	0.5398952666465107
1101	42-43	0.5078463035845431
1101	44-45	0.5406441636712742
1101	46-47	0.5711566944289999
1101	48-49	0.5351384912102475
1101	50-51	0.5048768926348401
1101	52-53	0.49594659897294235
1101	54-55	0.4086236640382026
1101	56-57	0.4249544175001674
1101	58-59	0.4688409535921423
1101	60-61	0.4329129606765889
1101	62-63	0.45850201661435364
1101	64-65	0.4272925918026047
1101	66-67	0.4139765359373371
1101	68-69	0.43538118947616056
1101	70-71	0.43528446817055055
1101	72-73	0.45394400296708426
1101	74-75	0.47522097755917514
1101	76-77	0.44243775596415347
1101	78-79	0.4422064473016505
1101	80-81	0.4024092719526351
1101	82-83	0.44048259671088275
1101	84-85	0.40364741243602253
1101	86-87	0.4431261531306738
1101	88-89	0.3924976761852115
1101	90-91	0.40378924879392386
1101	92-93	0.3432743538636238
1101	94-95	0.37796572915915405
1101	96-97	0.3347967710049211
1101	98-99	0.3115763326508443
1101	100	0.25120245525956975
1102	1	0.3481740061965688
1102	2	0.34513369984673403
1102	3	0.32860779720621736
1102	4	0.2989884984978275
1102	5	0.2738247480551763
1102	6	0.2294842663728005
1102	7	0.2367608169642068
1102	8	0.2564284656236495
1102	9	0.2388515762655672
1102	10-11	0.33058637023517434
1102	12-13	0.3691792994185832
1102	14-15	0.5141057302010452
1102	16-17	0.5572886985808054
1102	18-19	0.5806053023246065
1102	20-21	0.5278928539728724
1102	22-23	0.5316502212171628
1102	24-25	0.5002517390849093
1102	26-27	0.531862871319781
1102	28-29	0.5533384239585146
1102	30-31	0.5138570599837493
1102	32-33	0.4911263237124146
1102	34-35	0.5339367430268993
1102	36-37	0.5054558049851181
1102	38-39	0.5262559701627296
1102	40-41	0.5134318020506612
1102	42-43	0.5324903610767393
1102	44-45	0.5464533996514547
1102	46-47	0.5561498401857534
1102	48-49	0.5221973432371811
1102	50-51	0.4569674331262945
1102	52-53	0.43553494364510215
1102	54-55	0.4174304731914802
1102	56-57	0.41523978337310297
1102	58-59	0.43680966822119416
1102	60-61	0.3876257058794863
1102	62-63	0.42197877399201644
1102	64-65	0.4126795742472069
1102	66-67	0.37665987778465393
1102	68-69	0.38503454442157903
1102	70-71	0.40445495601064607
1102	72-73	0.3654561320446348
1102	74-75	0.40186848839795886
1102	76-77	0.3576487215532431
1102	78-79	0.38633184420375954
1102	80-81	0.37419372061397027
1102	82-83	0.4007361102544955
1102	84-85	0.38702758069474186
1102	86-87	0.38369337070068
1102	88-89	0.40412196542056833
1102	90-91	0.35055534936472554
1102	92-93	0.2992686250576462
1102	94-95	0.32665584307032347
1102	96-97	0.36442540229258213
1102	98-99	0.3367692365514543
1102	100	0.22489012258035146
1103	1	0.35695586051725314
1103	2	0.30182750830760696
1103	3	0.30943958563626595
1103	4	0.24162918856046645
1103	5	0.2667228133137698
1103	6	0.2613683176460597
1103	7	0.2698341046766686
1103	8	0.26025164649261256
1103	9	0.34033431322719565
1103	10-11	0.40878016657014626
1103	12-13	0.4111877574856422
1103	14-15	0.5407290249264918
1103	16-17	0.5690730159690176
1103	18-19	0.5500664831446826
1103	20-21	0.5307897008426679
1103	22-23	0.5170149090798191
1103	24-25	0.5219019031747081
1103	26-27	0.5484658760734504
1103	28-29	0.5774650385300291
1103	30-31	0.5514136368326916
1103	32-33	0.5615761766451826
1103	34-35	0.5758437130475116
1103	36-37	0.592758374716233
1103	38-39	0.6002005476502603
1103	40-41	0.5895229186838407
1103	42-43	0.5903203224514968
1103	44-45	0.5986323957375461
1103	46-47	0.6226843071345272
1103	48-49	0.5842782803036428
1103	50-51	0.5404770420896909
1103	52-53	0.5324802798016037
1103	54-55	0.5113009781439786
1103	56-57	0.5161638676596283
1103	58-59	0.519865979993277
1103	60-61	0.48524198237343086
1103	62-63	0.4808651991664661
1103	64-65	0.45216946841492955
1103	66-67	0.43836572211077396
1103	68-69	0.4375969046958019
1103	70-71	0.4568885582075737
1103	72-73	0.4602937202632873
1103	74-75	0.48569757148473514
1103	76-77	0.44416423479204425
1103	78-79	0.49347282913604573
1103	80-81	0.4637204346241681
1103	82-83	0.44852968013656636
1103	84-85	0.40190390395411413
1103	86-87	0.4373846743365206
1103	88-89	0.41376906662115687
1103	90-91	0.3847454599442912
1103	92-93	0.33580172761780247
1103	94-95	0.3231493539334629
1103	96-97	0.29753577045329394
1103	98-99	0.30399647089673465
1103	100	0.22836052176563726
1104	1	0.2362951288654891
1104	2	0.2720599243591124
1104	3	0.2593525120621578
1104	4	0.21152924955364938
1104	5	0.16769373768560314
1104	6	0.13266975983270157
1104	7	0.17396225257870412
1104	8	0.17941373454153364
1104	9	0.2353710861632763
1104	10-11	0.2907401316589997
1104	12-13	0.3367928406388856
1104	14-15	0.4061190988627388
1104	16-17	0.45762277823444464
1104	18-19	0.42704425629701603
1104	20-21	0.41343789877019077
1104	22-23	0.4149234716654533
1104	24-25	0.39766402754821684
1104	26-27	0.42846057193857234
1104	28-29	0.4451679932284165
1104	30-31	0.4094500176648097
1104	32-33	0.4390075312845525
1104	34-35	0.4479914236264335
1104	36-37	0.40659199550774616
1104	38-39	0.49702323701063733
1104	40-41	0.48241970483483954
1104	42-43	0.4436867049382016
1104	44-45	0.4927008706673064
1104	46-47	0.4545535039967632
1104	48-49	0.41796173845507667
1104	50-51	0.3875341381080091
1104	52-53	0.3909556645596055
1104	54-55	0.41645583532653774
1104	56-57	0.368843950003928
1104	58-59	0.3541005970514206
1104	60-61	0.3147867972637499
1104	62-63	0.35115783249732146
1104	64-65	0.34362511377911886
1104	66-67	0.3140942117094738
1104	68-69	0.3707673814103174
1104	70-71	0.3757134276331797
1104	72-73	0.3760375889515757
1104	74-75	0.37102519769092623
1104	76-77	0.3601422517045556
1104	78-79	0.39360948339103885
1104	80-81	0.3934605381170364
1104	82-83	0.36926916961596845
1104	84-85	0.3629989624269925
1104	86-87	0.39538593566280156
1104	88-89	0.37779707503323934
1104	90-91	0.30597794255813326
1104	92-93	0.28857246725821994
1104	94-95	0.28300513583470277
1104	96-97	0.2486918742037254
1104	98-99	0.23237128561703457
1104	100	0.20952024694232918
1105	1	0.17607962301057611
1105	2	0.21804593165424535
1105	3	0.21052492420252378
1105	4	0.1476582649857079
1105	5	0.1343194305521891
1105	6	0.14353823699124035
1105	7	0.16989673681674589
1105	8	0.09676809167913092
1105	9	0.15679185620717107
1105	10-11	0.17704916742482624
1105	12-13	0.21544026274701622
1105	14-15	0.3040934309088428
1105	16-17	0.35027095559656374
1105	18-19	0.3304983306956544
1105	20-21	0.31798385426616704
1105	22-23	0.31547448069709105
1105	24-25	0.31950280468775105
1105	26-27	0.3193221322481108
1105	28-29	0.34556963824758924
1105	30-31	0.3514809172518696
1105	32-33	0.34687155314009743
1105	34-35	0.35741203300306523
1105	36-37	0.3146809404410149
1105	38-39	0.3515287630506094
1105	40-41	0.35429503764143533
1105	42-43	0.34738853177314155
1105	44-45	0.34738080042009756
1105	46-47	0.36198612758613535
1105	48-49	0.31162709099719876
1105	50-51	0.25349639254061884
1105	52-53	0.2359847237535604
1105	54-55	0.25681943786753436
1105	56-57	0.27110169580951293
1105	58-59	0.23605979977006797
1105	60-61	0.2208525535369006
1105	62-63	0.21712380367078055
1105	64-65	0.23895496557771878
1105	66-67	0.21746782431161193
1105	68-69	0.2240544725702165
1105	70-71	0.21825717509815235
1105	72-73	0.21509089783090474
1105	74-75	0.2658665840437564
1105	76-77	0.22403914404130987
1105	78-79	0.23195015742464875
1105	80-81	0.18677898732196851
1105	82-83	0.15784007441687464
1105	84-85	0.17323720390900021
1105	86-87	0.14927268154569617
1105	88-89	0.1906360461510701
1105	90-91	0.1294419347440332
1105	92-93	0.11844849162729432
1105	94-95	0.17607377548386793
1105	96-97	0.21311882122607528
1105	98-99	0.21707048212805802
1105	100	0.19927855805406125
1106	1	0.0930449815871377
1106	2	0.12863660316947545
1106	3	0.1202755132426141
1106	4	0.10534606716790051
1106	5	0.12955507647951947
1106	6	0.10009764025142687
1106	7	0.04433358751407468
1106	8	0.05652664130139584
1106	9	0.11695541021743594
1106	10-11	0.17766824208884913
1106	12-13	0.19483586082244386
1106	14-15	0.2209592686295494
1106	16-17	0.2243181434693824
1106	18-19	0.2484465831536653
1106	20-21	0.23333369143210803
1106	22-23	0.20557453746280885
1106	24-25	0.24873286365679093
1106	26-27	0.25315465842372475
1106	28-29	0.23956364711526135
1106	30-31	0.2331599129476274
1106	32-33	0.24666397244210003
1106	34-35	0.2545631073322028
1106	36-37	0.23244276413429077
1106	38-39	0.2665528720805952
1106	40-41	0.2285667941515186
1106	42-43	0.25332750591131514
1106	44-45	0.26978318516317756
1106	46-47	0.293890178264661
1106	48-49	0.2479054674513037
1106	50-51	0.2673447814049723
1106	52-53	0.2592734656933757
1106	54-55	0.25393582231176026
1106	56-57	0.21147380346656774
1106	58-59	0.23456931325264208
1106	60-61	0.21225842692665253
1106	62-63	0.20958133594458417
1106	64-65	0.19569881558126667
1106	66-67	0.16716928908542172
1106	68-69	0.17837549522725737
1106	70-71	0.1836172853579754
1106	72-73	0.16586590933836476
1106	74-75	0.19559449216792757
1106	76-77	0.19228931119623027
1106	78-79	0.18129410070896057
1106	80-81	0.21910332006333633
1106	82-83	0.19513516118547258
1106	84-85	0.18332032016404298
1106	86-87	0.2348098629253812
1106	88-89	0.2356832373803428
1106	90-91	0.2439106278068941
1106	92-93	0.21546181519475383
1106	94-95	0.19240605231419394
1106	96-97	0.16602790727547756
1106	98-99	0.1808920761439552
1106	100	0.22050030302063206
1107	1	0.10964963476352452
1107	2	0.12120098715912775
1107	3	0.1470872170553541
1107	4	0.15300789487301358
1107	5	0.12231328768486804
1107	6	0.09262699625114124
1107	7	0.04872705875400385
1107	8	0.07245527234968563
1107	9	0.1263042378818895
1107	10-11	0.1272343240019964
1107	12-13	0.18417758466733858
1107	14-15	0.20195289945312567
1107	16-17	0.25497082127830595
1107	18-19	0.2029554983291746
1107	20-21	0.17919930210377544
1107	22-23	0.19136849430829272
1107	24-25	0.17725253271941455
1107	26-27	0.21922403583110395
1107	28-29	0.2094101926000036
1107	30-31	0.13925673787957749
1107	32-33	0.1500749220340154
1107	34-35	0.17310536698482082
1107	36-37	0.20003881886606933
1107	38-39	0.20466732683168942
1107	40-41	0.20178417221930545
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2216	70-71	-0.4796421743422101
2216	72-73	-0.6019927431764174
2216	74-75	-0.5591699551333456
2216	76-77	-0.4969440025268703
2216	78-79	-0.5416899759732807
2216	80-81	-0.5730933425234959
2216	82-83	-0.5441125791367334
2216	84-85	-0.6006365241886513
2216	86-87	-0.5111364450647926
2216	88-89	-0.5370909813564566
2216	90-91	-0.47347896214752794
2216	92-93	-0.4953455964641442
2216	94-95	-0.4671302690291341
2216	96-97	-0.4636724057355899
2216	98-99	-0.44597499525593065
2216	100	-0.48758763138025074
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	6.0
4	19.0
5	500.0
6	3512.0
7	7369.0
8	10558.0
9	15110.0
10	25467.0
11	41677.0
12	54974.0
13	65270.0
14	71322.0
15	76422.0
16	81427.0
17	86034.0
18	92247.0
19	99097.0
20	106741.0
21	113365.0
22	122030.0
23	139975.0
24	196324.0
25	224873.0
26	195708.0
27	314306.0
28	250744.0
29	364351.0
30	376224.0
31	482226.0
32	618352.0
33	944179.0
34	1176824.0
35	2557821.0
36	2810494.0
37	2871942.0
38	1203024.0
39	286594.0
40	2044.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.21519336740145	27.64403987062328	27.109853423480274	29.030913338494997
2	14.765155149747944	28.692822437395225	29.548707883594755	26.993314529262076
3	12.903305403315194	30.556118564689065	26.625498527236473	29.915077504759264
4	11.853104418823404	29.101902575381466	26.799290746728488	32.24570225906664
5	11.544412064982733	29.234317649356328	27.929326448495416	31.29194383716552
6	11.322653704444605	26.527491341761156	28.37251463125981	33.777340322534435
7	15.579358816096867	30.23086118550796	23.961181225530282	30.228598772864895
8	13.557314235518378	29.134678118742407	21.367886962992824	35.94012068274638
9	10.734347466755853	27.58437356832181	22.933720295756007	38.74755866916633
10-11	15.775550230555208	40.977891455868885	21.978493323102388	21.26806499047352
12-13	16.03410126588494	42.330489147522115	20.16211800077463	21.473291585818316
14-15	18.12006271836105	40.50843417010129	19.576571193429295	21.794931918108364
16-17	17.535386087967208	39.41257198263404	21.078078432713134	21.973963496685613
18-19	17.84627988880181	36.80184719942351	23.302879935611436	22.048992976163234
20-21	15.97844913479419	36.45224346618854	25.19724285768095	22.37206454133631
22-23	16.454441436331482	37.99215695829485	25.55840019800443	19.99500140736923
24-25	17.10304967901428	38.6580274866895	23.96765792850191	20.271264905794308
26-27	19.393511482421577	39.48696682674349	22.29745149561588	18.822070195219055
28-29	18.88143687219762	40.79849432478272	23.22418926399889	17.095879539020764
30-31	19.25955569139496	40.60650160003424	23.060169635361643	17.07377307320916
32-33	19.932402969749106	40.81180025297605	22.33329784035482	16.92249893692003
34-35	20.38325490207048	40.69304207315879	21.91528615522708	17.008416869543648
36-37	21.216705157359105	40.685938265251544	21.176813786209227	16.920542791180125
38-39	21.855625233039156	41.185419152845	20.668267331174604	16.290688282941243
40-41	22.25532186141851	41.13694613731707	20.6274923729437	15.980239628320724
42-43	22.47647577371804	40.46833857855013	21.231260839480207	15.823924808251624
44-45	22.69528964279334	39.25902458265722	21.858192402022482	16.18749337252695
46-47	22.847700210977727	38.44926458641043	22.73196454937651	15.971070653235333
48-49	22.869087811168978	38.74620187561023	23.294433611110353	15.090276702110442
50-51	23.404897642492216	39.236965400799505	23.129021089021986	14.229115867686287
52-53	23.734650290825797	40.071975026629275	22.612426132782947	13.580948549761981
54-55	23.46797959806885	41.17875172398232	22.09898736841281	13.254281309536012
56-57	23.113602000889045	41.75004507227834	21.61891791910001	13.517435007732603
58-59	22.816318115090862	41.39061539745832	22.20388438166088	13.589182105789934
60-61	22.667039368634413	40.45789880314869	23.231608881234287	13.643452946982618
62-63	22.51122908958905	39.53467498110355	23.89378083104587	14.060315098261528
64-65	21.94382088508137	39.21900610389049	24.124149849885253	14.713023161142887
66-67	21.21655034334455	38.4964509382244	24.64171002664924	15.645288691781815
68-69	21.210379200622306	37.73232084038523	24.476603857865673	16.580696101126797
70-71	21.668058762805405	37.102998517273086	24.48167496112294	16.747267758798568
72-73	22.400932133591766	37.00981002351313	24.363698322327995	16.225559520567103
74-75	22.43119939888972	37.22909832277078	24.076898550456157	16.26280372788334
76-77	21.505569725855974	37.87555941258493	23.796150955454003	16.822719906105096
78-79	20.482383916466343	38.84651543475481	23.35497545795377	17.31612519082508
80-81	19.375313250721558	40.40467113010478	23.24757397379654	16.972441645377117
82-83	18.09779097767634	42.05915765083844	23.719315460483077	16.12373591100214
84-85	16.934721599301096	43.887632305572275	23.985833030295524	15.19181306483111
86-87	16.03581970080436	45.76289952416829	23.768043089623106	14.433237685404249
88-89	15.462639123337174	47.882551993433644	23.04674039330864	13.60806848992054
90-91	15.213328826640943	49.96960655816993	21.86497304719028	12.952091567998844
92-93	15.181535478653723	51.45826557092995	20.673410952990547	12.686787997425775
94-95	15.379007556960211	52.09517027241928	19.87466579311373	12.651156377506783
96-97	15.706919306509265	51.896196026207306	19.61134278218082	12.785541885102608
98-99	16.27280300971552	51.06007666274104	19.501899504179722	13.165220823363718
100	17.126706043463674	50.236225347773356	19.202489002477478	13.434579606285494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	903980.0
1	780391.5
2	408059.5
3	108123.5
4	54338.0
5	48495.0
6	39971.5
7	30320.5
8	23870.5
9	22195.0
10	24053.5
11	28843.5
12	37517.0
13	50501.5
14	69031.5
15	92326.5
16	118776.5
17	145957.0
18	171796.0
19	195882.5
20	216422.5
21	232974.5
22	245308.0
23	254147.0
24	260389.5
25	263134.5
26	261138.0
27	254077.0
28	244333.5
29	230865.0
30	214465.0
31	197330.5
32	180138.5
33	164499.5
34	149743.0
35	136341.5
36	124676.5
37	116411.5
38	111814.0
39	110698.5
40	115395.5
41	128951.5
42	155760.5
43	200785.0
44	268541.0
45	358757.5
46	461885.5
47	556835.5
48	624537.0
49	655560.0
50	659808.5
51	658970.5
52	667137.0
53	679581.0
54	673910.0
55	629436.0
56	539525.0
57	417774.5
58	290497.0
59	182430.5
60	105318.0
61	57929.5
62	32117.0
63	19169.0
64	12637.5
65	9234.0
66	7235.0
67	5850.0
68	4728.5
69	3879.0
70	3240.5
71	2658.0
72	2277.0
73	2020.5
74	1804.5
75	1614.5
76	1436.5
77	1333.5
78	1283.5
79	1203.5
80	1129.5
81	1069.0
82	1010.0
83	952.0
84	913.5
85	916.0
86	878.0
87	785.5
88	745.5
89	713.5
90	678.0
91	663.5
92	614.5
93	562.0
94	514.0
95	498.0
96	472.5
97	405.0
98	368.0
99	329.5
100	347.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0566122921550391E-4
2	5.407368789264024E-4
3	9.323049636662109E-5
4	7.582747037818516E-4
5	5.096600468041954E-4
6	9.01228131544004E-4
7	8.515052001484727E-4
8	0.0012119964527660743
9	5.158754132286367E-4
10-11	3.6981430225426366E-4
12-13	8.359667840873692E-4
14-15	0.0025265464515354316
16-17	0.0012803654834349298
18-19	0.003632881675086002
20-21	0.0038162349846070235
22-23	0.0039001424313369823
24-25	0.005118354250527498
26-27	0.007156994437744279
28-29	0.009260895972417695
30-31	0.009518833679032014
32-33	0.009117942544655543
34-35	0.011318182258907801
36-37	0.010671784150765895
38-39	0.009089973395745557
40-41	0.007455332026117466
42-43	0.004279279783227908
44-45	0.005109031200890836
46-47	0.007470870442178571
48-49	0.0059170288360682185
50-51	0.0038504194999414514
52-53	0.004095926473706887
54-55	0.0042202338021957156
56-57	0.006883518315068857
58-59	0.008036468786802739
60-61	0.007672869850972916
62-63	0.009173880842475515
64-65	0.010127939588627271
66-67	0.009034035097925584
68-69	0.011604089114432106
70-71	0.011840273038560879
72-73	0.013620975519163341
74-75	0.01816751605864223
76-77	0.01918062078582618
78-79	0.019199266885099504
80-81	0.023605961680028462
82-83	0.021819043833001556
84-85	0.021315599152621805
86-87	0.0213000607365607
88-89	0.02187187444760931
90-91	0.02143679879789841
92-93	0.022002397142522577
94-95	0.019419912393167173
96-97	0.02169473650451273
98-99	0.018549761093745377
100	0.018851206365330785
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.6089156E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.96871327120436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.26109885712954	79.98951895834163
2	4.257154446101268	7.149355620718205
3	0.3424422840222436	0.8626331387700029
4	0.06201491768695923	0.20829251367174476
5	0.019180909884396097	0.08052981611818326
6	0.009551621068047004	0.048122239844282017
7	0.006820380568077741	0.040088900622498966
8	0.005035765043165418	0.03382773688085688
9	0.00315633109243965	0.0238529754441047
>10	0.027657574587483517	0.4504473765763625
>50	0.0030706258498425683	0.18442678587846117
>100	0.001897426381352835	0.3370755190030841
>500	3.728310435423438E-4	0.21949844544474334
>1k	2.877788283366262E-4	0.5141831271690975
>5k	8.854310170849332E-5	0.5363748848833851
>10k+	1.697076116079455E-4	9.321771960633367
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	645516	4.012118472839719	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	149806	0.9310991825798692	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124417	0.7732972444297265	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	113517	0.7055497504033151	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56016	0.34815996563150986	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47246	0.2936512020891587	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40271	0.25029902127867987	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39647	0.24642063262982844	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34685	0.21557998443175017	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33125	0.2058840128096216	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27918	0.1735205998375552	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24931	0.1549553003277487	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21979	0.13660753864279768	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19248	0.1196333729376482	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18869	0.1172777490627849	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0017713794309658008	0.0	0.0	0.0	0.0
2	0.004785832146819883	0.0	0.0	2.4861465697765627E-5	0.0
3	0.008863112521253446	0.0	0.0	5.5938297819972655E-5	6.215366424441407E-6
4	0.014879587220112726	0.0	0.0	8.079976351773829E-5	6.215366424441407E-6
5	0.023525161916510724	0.0	0.0	8.701512994217969E-5	6.215366424441407E-6
6	0.03839853377019901	0.0	0.0	8.701512994217969E-5	6.215366424441407E-6
7	0.058051522404282734	0.0	0.0	8.701512994217969E-5	6.215366424441407E-6
8	0.12995709656864537	0.0	0.0	9.323049636662109E-5	6.215366424441407E-6
9	0.39198451429024617	0.0	0.0	1.2430732848882814E-4	6.215366424441407E-6
10-11	0.9719527860877226	0.0	0.0	1.2741501170104885E-4	6.215366424441407E-6
12-13	1.737629991281084	0.0	0.0	1.3052269491326953E-4	6.215366424441407E-6
14-15	2.6152428380954227	0.0	0.0	1.3984574454993165E-4	6.215366424441407E-6
16-17	3.5236932254246276	0.0	0.0	1.5538416061103516E-4	6.215366424441407E-6
18-19	4.4841910911921055	0.0	0.0	1.6159952703547658E-4	6.215366424441407E-6
20-21	5.70770772562588	0.0	0.0	1.6159952703547658E-4	6.215366424441407E-6
22-23	7.174506232645143	0.0	0.0	1.6781489345991796E-4	6.215366424441407E-6
24-25	8.847372105783547	0.0	0.0	1.6781489345991796E-4	6.215366424441407E-6
26-27	10.888874469238784	0.0	3.1076832122207033E-6	1.6781489345991796E-4	6.215366424441407E-6
28-29	13.690149439784163	0.0	6.215366424441407E-6	1.8646099273324218E-4	6.215366424441407E-6
30-31	17.32322068354611	0.0	6.215366424441407E-6	1.926763591576836E-4	6.215366424441407E-6
32-33	21.211634718440173	0.0	6.215366424441407E-6	1.926763591576836E-4	6.215366424441407E-6
34-35	24.460652255469462	0.0	6.215366424441407E-6	1.926763591576836E-4	6.215366424441407E-6
36-37	26.76992814290569	0.0	6.215366424441407E-6	1.926763591576836E-4	6.215366424441407E-6
38-39	28.395659784764348	0.0	6.215366424441407E-6	2.206455080676699E-4	6.215366424441407E-6
40-41	29.749885575104127	0.0	6.215366424441407E-6	2.4550697376543557E-4	6.215366424441407E-6
42-43	30.841611579874048	0.0	6.215366424441407E-6	2.486146569776563E-4	6.215366424441407E-6
44-45	31.728336153866618	0.0	6.215366424441407E-6	2.5172234018987693E-4	6.215366424441407E-6
46-47	32.483108498668294	0.0	6.215366424441407E-6	2.6415307303875976E-4	6.215366424441407E-6
48-49	33.1694558745033	0.0	6.215366424441407E-6	2.983375883731875E-4	6.215366424441407E-6
50-51	33.8570991542378	0.0	6.215366424441407E-6	3.542758861931601E-4	6.215366424441407E-6
52-53	34.595273363003	0.0	6.215366424441407E-6	4.008911343764707E-4	6.215366424441407E-6
54-55	35.375938924328906	0.0	6.215366424441407E-6	4.5061406577200195E-4	6.215366424441407E-6
56-57	36.205155820479334	0.0	6.215366424441407E-6	4.630447986208848E-4	6.215366424441407E-6
58-59	37.067059328655894	0.0	6.215366424441407E-6	4.785832146819883E-4	6.215366424441407E-6
60-61	37.92881056035506	0.0	6.215366424441407E-6	5.158754132286367E-4	9.323049636662109E-6
62-63	38.740711445646994	0.0	6.215366424441407E-6	5.624906614119473E-4	1.2430732848882813E-5
64-65	39.50163700320887	0.0	6.215366424441407E-6	6.122135928074786E-4	1.2430732848882813E-5
66-67	40.19117286201961	0.0	6.215366424441407E-6	6.681518906274512E-4	1.2430732848882813E-5
68-69	40.809008253757995	0.0	6.215366424441407E-6	7.147671388107618E-4	1.2430732848882813E-5
70-71	41.37826744920616	0.0	6.215366424441407E-6	7.334132380840859E-4	1.2430732848882813E-5
72-73	41.92136616737385	6.215366424441407E-6	1.2430732848882813E-5	7.613823869940723E-4	1.2430732848882813E-5
74-75	42.43769530235147	6.215366424441407E-6	1.2430732848882813E-5	7.924592191162793E-4	1.2430732848882813E-5
76-77	42.93781786937736	6.215366424441407E-6	1.2430732848882813E-5	8.515052001484727E-4	1.2430732848882813E-5
78-79	43.42393100048256	6.215366424441407E-6	1.2430732848882813E-5	9.291972804539902E-4	1.2430732848882813E-5
80-81	43.89472946871794	6.215366424441407E-6	1.2430732848882813E-5	0.0010503969257305977	1.2430732848882813E-5
82-83	44.35908260197117	6.215366424441407E-6	1.2430732848882813E-5	0.0011001198571261289	1.2430732848882813E-5
84-85	44.82400754893544	6.215366424441407E-6	1.2430732848882813E-5	0.0011218736396116737	1.2430732848882813E-5
86-87	45.291064366583306	1.2430732848882813E-5	1.2430732848882813E-5	0.001140519738884998	1.2430732848882813E-5
88	45.639814792025135	1.2430732848882813E-5	1.2430732848882813E-5	0.0011498427885216602	1.2430732848882813E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	10670	0.0	50.782223	9
GCTTGCT	2225	0.0	45.62061	7
GTTGCTG	2605	0.0	44.91931	8
CTTGCTG	6695	0.0	42.115513	8
CCTGCTG	6130	0.0	41.70421	8
CTGCTGA	8920	0.0	40.830406	9
GCCTGCT	1995	0.0	39.10232	7
ATTGCTG	1745	0.0	37.164223	8
ACTGCTG	1410	0.0	36.6619	8
ACTTGCT	1580	0.0	35.69123	7
TACCCGA	11585	0.0	35.53411	6
TTTGCTG	3000	0.0	34.775482	8
AGTTGCT	945	0.0	34.31268	7
ACCCGAC	12565	0.0	34.07166	7
CCCGACG	14390	0.0	32.657326	8
TGCTTGC	1040	0.0	32.53393	6
CTACCCG	11735	0.0	32.196423	5
ATGCTGA	2565	0.0	31.696081	9
CATGCTG	1320	0.0	31.329262	8
ACTGCCT	1305	0.0	30.248753	4
>>END_MODULE