##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248491_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16089156 Sequences flagged as poor quality 0 Sequence length 100 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2646524155773 34.0 31.0 34.0 30.0 34.0 2 32.57047156482292 34.0 31.0 34.0 31.0 34.0 3 32.704071736267586 34.0 31.0 34.0 31.0 34.0 4 36.18969851495007 37.0 35.0 37.0 35.0 37.0 5 36.10637406959072 37.0 35.0 37.0 35.0 37.0 6 36.10551964316836 37.0 36.0 37.0 35.0 37.0 7 36.096573617659 37.0 36.0 37.0 35.0 37.0 8 36.08539347868838 37.0 36.0 37.0 35.0 37.0 9 37.830584152456474 39.0 38.0 39.0 35.0 39.0 10-11 37.84372303307893 39.0 38.0 39.0 35.0 39.0 12-13 37.83723285422803 39.0 38.0 39.0 35.0 39.0 14-15 39.26480233021546 41.0 39.0 41.0 36.0 41.0 16-17 39.15941016421247 41.0 39.0 41.0 36.0 41.0 18-19 38.94938824012894 40.0 38.0 41.0 35.5 41.0 20-21 38.70921852581951 40.0 38.0 41.0 35.0 41.0 22-23 38.49958935074034 40.0 37.5 41.0 35.0 41.0 24-25 38.30619294138238 40.0 37.0 41.0 34.0 41.0 26-27 38.12941219539422 40.0 36.0 41.0 34.0 41.0 28-29 37.973925729851835 40.0 36.0 41.0 33.5 41.0 30-31 37.90746944712326 40.0 36.0 41.0 33.0 41.0 32-33 37.82789979785142 40.0 35.0 41.0 33.0 41.0 34-35 37.79621435704893 40.0 35.0 41.0 33.0 41.0 36-37 37.714192559261654 40.0 35.0 41.0 33.0 41.0 38-39 37.64905517728835 40.0 35.0 41.0 33.0 41.0 40-41 37.505677302153074 40.0 35.0 41.0 33.0 41.0 42-43 37.32793889250623 39.5 35.0 41.0 32.5 41.0 44-45 37.072557845793774 39.0 35.0 41.0 32.0 41.0 46-47 36.761904477773726 39.0 35.0 41.0 31.5 41.0 48-49 36.4075899320014 38.0 35.0 40.0 31.0 41.0 50-51 36.022354342266304 37.0 35.0 40.0 31.0 41.0 52-53 35.63491506328859 36.5 35.0 39.0 30.5 41.0 54-55 35.31912705675798 35.5 35.0 39.0 30.0 41.0 56-57 35.0058144131364 35.0 35.0 38.5 30.0 41.0 58-59 34.683998930708356 35.0 34.0 37.0 30.0 40.0 60-61 34.38905120318306 35.0 34.0 37.0 30.0 40.0 62-63 34.13254209854141 35.0 34.0 36.0 29.0 40.0 64-65 33.911760163180716 35.0 34.0 35.5 29.0 39.0 66-67 33.71040833341414 35.0 34.0 35.0 29.0 39.0 68-69 33.53230843805604 35.0 33.0 35.0 29.0 38.5 70-71 33.41064767474441 35.0 33.0 35.0 29.0 37.5 72-73 33.28677725543838 35.0 33.0 35.0 29.0 37.0 74-75 33.154194570554225 35.0 33.0 35.0 29.0 36.5 76-77 33.02125043103567 35.0 33.0 35.0 28.5 36.0 78-79 32.88000790718917 35.0 33.0 35.0 27.5 36.0 80-81 32.751270110128836 35.0 33.0 35.0 27.0 36.0 82-83 32.65850132225705 35.0 33.0 35.0 27.0 35.0 84-85 32.56971726795364 35.0 33.0 35.0 27.0 35.0 86-87 32.49644030426457 35.0 33.0 35.0 27.0 35.0 88-89 32.40895731261478 35.0 33.0 35.0 27.0 35.0 90-91 32.345945617035476 35.0 33.0 35.0 27.0 35.0 92-93 32.24008130072205 35.0 33.0 35.0 25.5 35.0 94-95 32.06911353833601 35.0 33.0 35.0 25.0 35.0 96-97 31.934361597339226 35.0 33.0 35.0 24.5 35.0 98-99 31.781466411289692 35.0 33.0 35.0 24.0 35.0 100 30.560945024089516 34.0 30.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2829570094994054 1101 2 0.2622841848635531 1101 3 0.24305076140657178 1101 4 0.17675628626223983 1101 5 0.17852981599843076 1101 6 0.18027119988673945 1101 7 0.2084742753116373 1101 8 0.2035737801905526 1101 9 0.2678327396099007 1101 10-11 0.2790777437746996 1101 12-13 0.2726567096602608 1101 14-15 0.3758706352103687 1101 16-17 0.38418154685109585 1101 18-19 0.3934769916404761 1101 20-21 0.37656422172391046 1101 22-23 0.37401020344579905 1101 24-25 0.37866279195572616 1101 26-27 0.4128707484136953 1101 28-29 0.37524185288570777 1101 30-31 0.37081005933500677 1101 32-33 0.4006304173868358 1101 34-35 0.40607155883007806 1101 36-37 0.4068842352982429 1101 38-39 0.413306112628824 1101 40-41 0.4098306277993018 1101 42-43 0.3881838525733414 1101 44-45 0.38517691066351034 1101 46-47 0.39397132869115836 1101 48-49 0.3805763512888518 1101 50-51 0.3452182487138913 1101 52-53 0.336727445282186 1101 54-55 0.35953496390901307 1101 56-57 0.34476445864009264 1101 58-59 0.35120140726282756 1101 60-61 0.36545210662058025 1101 62-63 0.34542130495838563 1101 64-65 0.34177697382305894 1101 66-67 0.3421198041883713 1101 68-69 0.3801303456465348 1101 70-71 0.35697884699954585 1101 72-73 0.3380980956548427 1101 74-75 0.31832476295388545 1101 76-77 0.34574656595589914 1101 78-79 0.3743032575540397 1101 80-81 0.39101227982772 1101 82-83 0.3635127466933312 1101 84-85 0.34427805080797214 1101 86-87 0.306561425006592 1101 88-89 0.3499564325511457 1101 90-91 0.34593729782842786 1101 92-93 0.35329258383348616 1101 94-95 0.3635563157039883 1101 96-97 0.3917852974160212 1101 98-99 0.3799710143999988 1101 100 0.37067601442845444 1102 1 0.2198771427936066 1102 2 0.23790901500191097 1102 3 0.23134789286217483 1102 4 0.1410292238270614 1102 5 0.1773276148289895 1102 6 0.16881943138267275 1102 7 0.17517551942381715 1102 8 0.19194213940379257 1102 9 0.2739737992842137 1102 10-11 0.2293244038604101 1102 12-13 0.23914257460053534 1102 14-15 0.336899978153248 1102 16-17 0.3580559189925836 1102 18-19 0.3523460711316062 1102 20-21 0.33593592242498715 1102 22-23 0.35220038811920773 1102 24-25 0.35404159267792323 1102 26-27 0.33527156427905425 1102 28-29 0.382500214066674 1102 30-31 0.36754461834713226 1102 32-33 0.35777523426048674 1102 34-35 0.4191183487902421 1102 36-37 0.42166972009012227 1102 38-39 0.4200339988816495 1102 40-41 0.4107101460563669 1102 42-43 0.4043970305002276 1102 44-45 0.387816404439171 1102 46-47 0.39602947853624215 1102 48-49 0.3940482097947964 1102 50-51 0.36618699488666095 1102 52-53 0.36773651672328356 1102 54-55 0.3857649145956117 1102 56-57 0.3561676026469769 1102 58-59 0.35693145728016873 1102 60-61 0.40938896257832624 1102 62-63 0.37241611633378824 1102 64-65 0.3895392361282788 1102 66-67 0.41249632866129105 1102 68-69 0.4178538556581941 1102 70-71 0.3875747246023593 1102 72-73 0.35563006837593036 1102 74-75 0.36120904269890985 1102 76-77 0.3730145160912173 1102 78-79 0.418436967548125 1102 80-81 0.41182794778367793 1102 82-83 0.385904585448273 1102 84-85 0.2800845509730081 1102 86-87 0.33980283446837234 1102 88-89 0.3791056570958702 1102 90-91 0.40987449269280773 1102 92-93 0.42384729265740617 1102 94-95 0.4278041776380306 1102 96-97 0.3987073310959346 1102 98-99 0.40234040582614483 1102 100 0.44537288483307336 1103 1 0.16393694327975084 1103 2 0.20428945474102278 1103 3 0.18678791600249411 1103 4 0.12400743673189396 1103 5 0.12203443379475232 1103 6 0.13773002759691622 1103 7 0.16371534521059772 1103 8 0.171056908982969 1103 9 0.21469897089419732 1103 10-11 0.21890764044020017 1103 12-13 0.20992134534365903 1103 14-15 0.2865935173344596 1103 16-17 0.3055300716539975 1103 18-19 0.2955675323654887 1103 20-21 0.3020181259511361 1103 22-23 0.29879891943244985 1103 24-25 0.30224977737265846 1103 26-27 0.3160263166028159 1103 28-29 0.29135237958806925 1103 30-31 0.2981175159784968 1103 32-33 0.3013557916484544 1103 34-35 0.3695828721514829 1103 36-37 0.3893101114135078 1103 38-39 0.3692791554919026 1103 40-41 0.3620711240827319 1103 42-43 0.3599740290756088 1103 44-45 0.3870812949323934 1103 46-47 0.3173714296147381 1103 48-49 0.3171082020257785 1103 50-51 0.32050037890075345 1103 52-53 0.32978947150133564 1103 54-55 0.29505314314214104 1103 56-57 0.28329133900347614 1103 58-59 0.33403827719532586 1103 60-61 0.3453974184341888 1103 62-63 0.3330311059847588 1103 64-65 0.34296667408877823 1103 66-67 0.36189865900658447 1103 68-69 0.3450685141141534 1103 70-71 0.3272832188770707 1103 72-73 0.3283793801165018 1103 74-75 0.34660651885754845 1103 76-77 0.3374136098079603 1103 78-79 0.36571402153960975 1103 80-81 0.3790736452207071 1103 82-83 0.3650553021813394 1103 84-85 0.36143108438598404 1103 86-87 0.3480624819779621 1103 88-89 0.33856933282951474 1103 90-91 0.35730570327731215 1103 92-93 0.4147582967407857 1103 94-95 0.3666684682191388 1103 96-97 0.35522363121489775 1103 98-99 0.3505411033785606 1103 100 0.37803442255735575 1104 1 0.2004899803510014 1104 2 0.18967879831408396 1104 3 0.1770559487062897 1104 4 0.11858286648079996 1104 5 0.15033905860376962 1104 6 0.14488254954893875 1104 7 0.14938049757421368 1104 8 0.14265388942479973 1104 9 0.2148029788479917 1104 10-11 0.21224157197653426 1104 12-13 0.21264958203293816 1104 14-15 0.2453677753347776 1104 16-17 0.27400268238162084 1104 18-19 0.2636028051453465 1104 20-21 0.2589143123661728 1104 22-23 0.23170922976957087 1104 24-25 0.24543694603622868 1104 26-27 0.2600561842828526 1104 28-29 0.27021674657665073 1104 30-31 0.2669712507735085 1104 32-33 0.24638727285608297 1104 34-35 0.27783658907394937 1104 36-37 0.29393757589272695 1104 38-39 0.3391526790397563 1104 40-41 0.30999560509437885 1104 42-43 0.2969421347458123 1104 44-45 0.27674578750805523 1104 46-47 0.2825257645146664 1104 48-49 0.2929587347764908 1104 50-51 0.2739395051249929 1104 52-53 0.2672889403382115 1104 54-55 0.22751480829852255 1104 56-57 0.24674114591550023 1104 58-59 0.26676376903157006 1104 60-61 0.2798893724347238 1104 62-63 0.2625101782868242 1104 64-65 0.26518521369222725 1104 66-67 0.3018360893135892 1104 68-69 0.3253836065678186 1104 70-71 0.3078605639694061 1104 72-73 0.3052993546381373 1104 74-75 0.2560938142821598 1104 76-77 0.2465215118022499 1104 78-79 0.28448336610671987 1104 80-81 0.3038284491065042 1104 82-83 0.27931657157568424 1104 84-85 0.23089403939536624 1104 86-87 0.24066356660993193 1104 88-89 0.24955251496120212 1104 90-91 0.2851976449983127 1104 92-93 0.2654298136257651 1104 94-95 0.28759198210673986 1104 96-97 0.294527929217395 1104 98-99 0.2794597978228097 1104 100 0.26172264950802315 1105 1 0.11792919701398574 1105 2 0.1466298778593469 1105 3 0.1344421241297198 1105 4 0.1040467063855317 1105 5 0.12799459218607012 1105 6 0.10816527599638448 1105 7 0.1139570419813225 1105 8 0.1079019205497147 1105 9 0.14114759650624364 1105 10-11 0.17147707943315282 1105 12-13 0.1709213336122275 1105 14-15 0.20687504834243242 1105 16-17 0.19275242894790523 1105 18-19 0.23289778171815811 1105 20-21 0.20934089627652241 1105 22-23 0.22092249317297785 1105 24-25 0.168197691064222 1105 26-27 0.20439440904736728 1105 28-29 0.22983558525661607 1105 30-31 0.21602846913626905 1105 32-33 0.19745023529932126 1105 34-35 0.22710464400918795 1105 36-37 0.23937721654345268 1105 38-39 0.2815777377511566 1105 40-41 0.2563268360200013 1105 42-43 0.24347055378996885 1105 44-45 0.2702592875620695 1105 46-47 0.2716333934755184 1105 48-49 0.22046793378662244 1105 50-51 0.19332490523972012 1105 52-53 0.21644572068142764 1105 54-55 0.20446208180933922 1105 56-57 0.17441384230907175 1105 58-59 0.20568228933777277 1105 60-61 0.22920447006141842 1105 62-63 0.2180142663440634 1105 64-65 0.2153985871364199 1105 66-67 0.24416304175208836 1105 68-69 0.2714918923416292 1105 70-71 0.2396267406306194 1105 72-73 0.22512955184113537 1105 74-75 0.21276458882420712 1105 76-77 0.2092565431047646 1105 78-79 0.2517671659978902 1105 80-81 0.20422545632938238 1105 82-83 0.2176663712314948 1105 84-85 0.22082433728627393 1105 86-87 0.20483323979730983 1105 88-89 0.23509243232376065 1105 90-91 0.21450289542330125 1105 92-93 0.20283368838721572 1105 94-95 0.1786588000517071 1105 96-97 0.1948581565732468 1105 98-99 0.16773075723704522 1105 100 0.13749857080955508 1106 1 0.07628144309354212 1106 2 0.08427075177763044 1106 3 0.10787550548320723 1106 4 0.06564690828989939 1106 5 0.06543887315103092 1106 6 0.07576966413242303 1106 7 0.09062502145867057 1106 8 0.06993798119803785 1106 9 0.10507747351429941 1106 10-11 0.09411016299477382 1106 12-13 0.11693825195151675 1106 14-15 0.15331531374314267 1106 16-17 0.14922113424719186 1106 18-19 0.15691890439887146 1106 20-21 0.13722971779140636 1106 22-23 0.1427151573250427 1106 24-25 0.11176945508793779 1106 26-27 0.13169853241572582 1106 28-29 0.134193117152428 1106 30-31 0.17692047353813223 1106 32-33 0.15507936670297084 1106 34-35 0.19505735882308528 1106 36-37 0.19346644396416934 1106 38-39 0.23127378855948422 1106 40-41 0.17438775806182605 1106 42-43 0.16605163897943243 1106 44-45 0.17112072983875493 1106 46-47 0.16628308042191975 1106 48-49 0.15794914037134333 1106 50-51 0.1542138376465445 1106 52-53 0.16630529607810018 1106 54-55 0.15852005073689668 1106 56-57 0.16366315952940624 1106 58-59 0.1451452374384914 1106 60-61 0.17242161336169204 1106 62-63 0.17956451541805052 1106 64-65 0.16912504848993848 1106 66-67 0.20280165908057768 1106 68-69 0.2279697998172523 1106 70-71 0.17972651965558128 1106 72-73 0.19004361339202092 1106 74-75 0.17679259752404874 1106 76-77 0.18528684932179829 1106 78-79 0.1983898372031021 1106 80-81 0.19584846462552008 1106 82-83 0.1727093725614779 1106 84-85 0.15258407968588017 1106 86-87 0.13971953944270865 1106 88-89 0.15411503886922873 1106 90-91 0.13412755131773935 1106 92-93 0.15407563025285498 1106 94-95 0.19104717379087788 1106 96-97 0.17334310098147654 1106 98-99 0.1840863423809651 1106 100 0.15380854644722675 1107 1 0.07553809611958684 1107 2 0.08767431720554697 1107 3 0.07173257838893221 1107 4 0.05709537857296709 1107 5 0.05400117813979932 1107 6 0.04175125268434243 1107 7 0.08378858541089329 1107 8 0.07846928350912208 1107 9 0.10043941779274945 1107 10-11 0.11482908345764997 1107 12-13 0.10458229864502044 1107 14-15 0.13766788847559042 1107 16-17 0.14695359916214557 1107 18-19 0.13893717482048373 1107 20-21 0.1278555274448152 1107 22-23 0.13880747613401212 1107 24-25 0.13787163031317107 1107 26-27 0.1819878173729279 1107 28-29 0.15056469436029118 1107 30-31 0.1466473896820375 1107 32-33 0.1489069196656132 1107 34-35 0.19014122279439505 1107 36-37 0.22487557234164512 1107 38-39 0.2009564349884272 1107 40-41 0.16370275365756726 1107 42-43 0.19867838523279602 1107 44-45 0.1858667328963648 1107 46-47 0.12394611243129106 1107 48-49 0.12072080195972035 1107 50-51 0.11939357695170116 1107 52-53 0.11044310116714229 1107 54-55 0.11902602133169182 1107 56-57 0.09219817770692629 1107 58-59 0.10372092103163055 1107 60-61 0.10981190819707365 1107 62-63 0.1097558573726829 1107 64-65 0.09346959081727135 1107 66-67 0.11050580865800441 1107 68-69 0.1257691055594421 1107 70-71 0.12076200906891899 1107 72-73 0.1563272075875517 1107 74-75 0.13271194136975595 1107 76-77 0.12320920220370368 1107 78-79 0.1291724661253042 1107 80-81 0.1543728592588991 1107 82-83 0.1077258094953848 1107 84-85 0.06263346963020666 1107 86-87 0.04128471654055943 1107 88-89 0.04672311019185571 1107 90-91 0.0763657862442031 1107 92-93 0.1444571070930607 1107 94-95 0.11752066520483595 1107 96-97 0.12426120676922636 1107 98-99 0.08793715076357955 1107 100 0.03421007043134594 1108 1 0.04173496699456791 1108 2 0.04654479137968082 1108 3 0.05081976571571545 1108 4 0.0074506065303552305 1108 5 0.03434108814797554 1108 6 0.019046904347987947 1108 7 4.6315158378718024E-5 1108 8 0.02817205209233009 1108 9 0.0194238004153533 1108 10-11 0.027862850648837423 1108 12-13 0.04406558527655591 1108 14-15 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2214 82-83 -0.279907063554397 2214 84-85 -0.25380054682344166 2214 86-87 -0.28924702295218907 2214 88-89 -0.3211954462546345 2214 90-91 -0.30227757901708685 2214 92-93 -0.324464755949883 2214 94-95 -0.3138591872354013 2214 96-97 -0.29685067499086415 2214 98-99 -0.29136303100722216 2214 100 -0.4502621911047022 2215 1 -0.2673200118792636 2215 2 -0.34846928735517935 2215 3 -0.315762879694212 2215 4 -0.19905806960374406 2215 5 -0.23508701350704087 2215 6 -0.2640287711939422 2215 7 -0.28906549637080303 2215 8 -0.300715709699908 2215 9 -0.4393812763202334 2215 10-11 -0.3796353503444365 2215 12-13 -0.35638311808715173 2215 14-15 -0.47204383241821546 2215 16-17 -0.4677819995911179 2215 18-19 -0.441928082730513 2215 20-21 -0.45546612825935284 2215 22-23 -0.41852255753519074 2215 24-25 -0.47371234971734566 2215 26-27 -0.5053823381057327 2215 28-29 -0.49575412432815114 2215 30-31 -0.5144847886888826 2215 32-33 -0.5230971456889861 2215 34-35 -0.5374005518979885 2215 36-37 -0.5300114845148443 2215 38-39 -0.5438673849127653 2215 40-41 -0.505270982225305 2215 42-43 -0.569243952358967 2215 44-45 -0.5587691919380333 2215 46-47 -0.5203284039745526 2215 48-49 -0.505191637595864 2215 50-51 -0.4767031724044486 2215 52-53 -0.4262001451259394 2215 54-55 -0.4306803376285302 2215 56-57 -0.4923108430805243 2215 58-59 -0.5212789167494307 2215 60-61 -0.5426403175142127 2215 62-63 -0.45049807733732905 2215 64-65 -0.49258617190324827 2215 66-67 -0.5635078334538974 2215 68-69 -0.5756771665004905 2215 70-71 -0.5023313570319559 2215 72-73 -0.4500900551156022 2215 74-75 -0.43000126803365646 2215 76-77 -0.41790322780430245 2215 78-79 -0.37075649976646474 2215 80-81 -0.3443365695496041 2215 82-83 -0.34955880827812535 2215 84-85 -0.3805309812936599 2215 86-87 -0.3816890552126324 2215 88-89 -0.33966535517752305 2215 90-91 -0.40439391202788144 2215 92-93 -0.4069525941299901 2215 94-95 -0.43656261117969564 2215 96-97 -0.39105456488973545 2215 98-99 -0.353912709893482 2215 100 -0.5110975313126431 2216 1 -0.313733922313844 2216 2 -0.3466582859101308 2216 3 -0.3521419596644364 2216 4 -0.22517537240515395 2216 5 -0.27841627321854645 2216 6 -0.3046234016067757 2216 7 -0.31118553827253237 2216 8 -0.34482881056046466 2216 9 -0.44283659658285757 2216 10-11 -0.4196348913113823 2216 12-13 -0.4388730130691414 2216 14-15 -0.5703814487964607 2216 16-17 -0.5960233123789536 2216 18-19 -0.6174973033684381 2216 20-21 -0.6181960917142462 2216 22-23 -0.604401986026808 2216 24-25 -0.5858596870156276 2216 26-27 -0.6243376835060985 2216 28-29 -0.6912521418416517 2216 30-31 -0.7273175215466026 2216 32-33 -0.6872971899401676 2216 34-35 -0.7070329533774782 2216 36-37 -0.6990935079678948 2216 38-39 -0.7104271725539988 2216 40-41 -0.7055950044901422 2216 42-43 -0.6949742191087083 2216 44-45 -0.7982275122832974 2216 46-47 -0.7440482209524149 2216 48-49 -0.6769067794599835 2216 50-51 -0.6619197233753908 2216 52-53 -0.6952711882283396 2216 54-55 -0.5821316353494836 2216 56-57 -0.4824870892144517 2216 58-59 -0.46733220309143775 2216 60-61 -0.5508822312218555 2216 62-63 -0.5050458294053968 2216 64-65 -0.5634339457192254 2216 66-67 -0.5023690982648077 2216 68-69 -0.5842644532775054 2216 70-71 -0.5075386205023378 2216 72-73 -0.5086582709041778 2216 74-75 -0.4657601365101556 2216 76-77 -0.512874670590115 2216 78-79 -0.49882615365781646 2216 80-81 -0.460554389659265 2216 82-83 -0.5007576697970748 2216 84-85 -0.5100602768049924 2216 86-87 -0.5315459886706151 2216 88-89 -0.5611583438115986 2216 90-91 -0.6154044902955249 2216 92-93 -0.656153573005767 2216 94-95 -0.6907024751231177 2216 96-97 -0.660438839096404 2216 98-99 -0.7199857536571912 2216 100 -0.7512648644656288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 3.0 6 82.0 7 269.0 8 595.0 9 1088.0 10 1655.0 11 2279.0 12 3385.0 13 4650.0 14 6625.0 15 9827.0 16 14954.0 17 21771.0 18 30371.0 19 39599.0 20 49792.0 21 61277.0 22 74026.0 23 89565.0 24 108445.0 25 128373.0 26 152610.0 27 180496.0 28 214909.0 29 260280.0 30 305273.0 31 371746.0 32 470882.0 33 637045.0 34 903797.0 35 2495417.0 36 2684944.0 37 4743575.0 38 1638009.0 39 379022.0 40 2520.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.969863207938797 23.11187246630262 31.00268260578755 26.915581719971033 2 16.661293156758987 23.54945534240322 37.35476993949782 22.434481561339968 3 14.234146278400184 21.910005720623257 38.61618968701652 25.239658313960035 4 14.744459855101152 19.292765841338465 40.79010985971606 25.17266444384432 5 14.024259417498513 20.688557191418884 42.37360939946145 22.913573991621156 6 13.20381877085411 15.587014011176223 39.087600368844704 32.12156684912497 7 18.373878654666534 16.603928757978355 36.441308667775985 28.580883919579126 8 15.26367200367751 16.55035229939967 36.68641164272383 31.49956405419899 9 15.611353386094336 15.782779407446853 38.86639547779884 29.73947172865997 10-11 18.73605427158516 24.157712188258973 38.11443558630422 18.99179795385165 12-13 17.145514034421694 30.72605237962762 32.94253906171337 19.18589452423732 14-15 18.97897876060124 27.08547297322495 32.98463573850611 20.950912527667704 16-17 24.732647218138204 26.654726932181248 34.086416081411464 14.526209768269084 18-19 24.728466179332216 21.06247835498643 34.05690453868432 20.152150926997038 20-21 19.558742271898645 20.426878347393657 39.91899128659874 20.09538809410896 22-23 19.272480793896214 22.553022669430266 41.03758146170004 17.13691507497348 24-25 19.937913452065704 24.591937046284006 37.85824095717652 17.611908544473767 26-27 23.41520276141272 24.62552728061062 33.937902025438746 18.021367932537917 28-29 21.240679785834626 27.91667654650985 36.39054579340724 14.45209787424828 30-31 21.00267084406821 26.098767374214678 36.381820036828024 16.516741744889096 32-33 21.469519879490665 26.78546344953746 36.088974584195306 15.656042086776568 34-35 21.589112916734233 26.738306586170868 35.99346104737481 15.679119449720094 36-37 22.15334042382335 26.025936351167207 35.42856753952787 16.392155685481573 38-39 22.89916183141334 26.3905136922913 34.384900708714696 16.325423767580663 40-41 23.219899861627134 26.655289217811994 34.3213374340712 15.803473486489672 42-43 23.309763886720845 26.132140038356976 34.850745881355046 15.707350193567137 44-45 23.422438258898914 24.678602315642653 35.40070707941173 16.4982523460467 46-47 23.259550912337993 24.09602246684493 36.08426508815998 16.560161532657094 48-49 22.272994760793626 24.646499320843617 36.003912094900656 17.0765938234621 50-51 22.326228198841832 24.933123365609454 35.65309070755462 17.087557727994092 52-53 22.196195368040385 25.604665976325723 35.6103489982054 16.588789657428485 54-55 21.981621087334936 26.135865947635317 35.74685802922225 16.135654935807498 56-57 21.56683443724086 25.85988077854654 35.619333656512104 16.9539511277005 58-59 21.34847288908358 25.602403432548094 35.80604028595534 17.243083392412984 60-61 21.00738842796913 25.245406895884752 35.772422732305905 17.974781943840217 62-63 20.69232220757882 25.180195903377406 35.77963940432923 18.347842484714548 64-65 20.351398247981052 25.05989672234134 35.96340142838474 18.62530360129286 66-67 19.51221369225334 24.538686180928323 37.12168929184352 18.827410834974813 68-69 18.832547068665015 23.662300702696985 38.52120503041987 18.98394719821813 70-71 18.089282027204437 23.12935787035535 39.871293382295995 18.91006672014422 72-73 17.91088683061325 22.559382260404327 40.9350903537701 18.594640555212326 74-75 17.82364534888581 22.310637517774847 41.30777014110146 18.55794699223788 76-77 17.733420675873766 22.396039913498193 41.21492545578949 18.655613954838554 78-79 17.394427736505424 22.589106967953835 41.39273738817004 18.6237279073707 80-81 17.022507638082786 22.34690519976362 41.80389401133039 18.826693150823203 82-83 17.26293931478791 22.058785002950927 41.90746626340196 18.770809418859205 84-85 17.78430365534847 22.068133170084188 41.537471093023235 18.610092081544106 86-87 18.34330706977878 22.334185608644468 41.113583248871336 18.208924072705422 88-89 18.636554859954845 22.78371243307877 40.54489126293747 18.034841444028913 90-91 18.824092342102546 22.860401579227812 40.099861838387895 18.215644240281744 92-93 18.51890997213647 22.752513653628256 40.07025550791124 18.658320866324033 94-95 18.078503073347886 23.00921693587104 40.46339144554383 18.44888854523725 96-97 17.491258743160305 23.700135927627667 41.048757521912464 17.75984780729957 98-99 17.306648886289445 24.584173651426394 40.87519048461835 17.23398697766581 100 17.249469696894355 25.523733363697932 40.387877067137914 16.8389198722698 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 264150.0 1 539363.0 2 679101.5 3 356380.5 4 108446.5 5 36803.5 6 22382.5 7 17590.0 8 16180.0 9 17213.5 10 20643.0 11 26960.0 12 36970.5 13 51421.5 14 71126.5 15 95455.5 16 122926.0 17 151689.5 18 179047.0 19 203031.5 20 221334.5 21 233730.0 22 242938.0 23 250060.0 24 254691.5 25 257106.5 26 255438.0 27 248106.0 28 237469.0 29 224049.0 30 208585.0 31 192078.0 32 175012.0 33 158854.0 34 143327.0 35 128513.0 36 114706.0 37 101882.0 38 90433.0 39 80039.0 40 71158.0 41 64426.5 42 61127.5 43 64613.0 44 78302.0 45 103874.0 46 141263.5 47 189823.0 48 254043.5 49 349931.0 50 491102.5 51 682310.5 52 904975.0 53 1101667.5 54 1198238.0 55 1142431.5 56 943174.0 57 674388.5 58 420522.0 59 231766.5 60 116044.0 61 54044.5 62 23998.0 63 10355.5 64 4671.0 65 2337.5 66 1407.5 67 986.5 68 759.0 69 574.0 70 426.5 71 335.5 72 236.5 73 167.5 74 139.0 75 105.5 76 80.5 77 58.0 78 35.5 79 25.0 80 18.0 81 11.5 82 7.0 83 3.5 84 5.0 85 6.5 86 4.0 87 2.0 88 1.5 89 1.0 90 1.0 91 0.5 92 0.5 93 1.0 94 1.0 95 1.0 96 0.5 97 1.5 98 1.5 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0599223476980396 2 0.006059982263830371 3 0.0 4 6.215366424441406E-6 5 3.9778345116424997E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.3052269491326953E-4 18-19 0.0 20-21 9.074434979684453E-4 22-23 0.0 24-25 1.5538416061103514E-5 26-27 0.0 28-29 3.107683212220703E-6 30-31 2.9522990516096684E-4 32-33 6.961210395374375E-4 34-35 2.175378248554492E-4 36-37 0.0 38-39 1.8646099273324218E-4 40-41 1.1809196206438672E-4 42-43 2.2064550806766994E-4 44-45 9.63381795788418E-5 46-47 1.5849184382325587E-4 48-49 4.847985811064297E-4 50-51 1.0255354600328321E-4 52-53 2.7969148909986327E-5 54-55 6.526134745663476E-5 56-57 1.3363037812549024E-4 58-59 3.107683212220703E-6 60-61 5.904598103219336E-5 62-63 0.0 64-65 2.1753782485544922E-5 66-67 0.0 68-69 1.2430732848882812E-5 70-71 0.0010068893607595077 72-73 0.0010939044907016875 74-75 3.6981430225426366E-4 76-77 7.769208030551758E-5 78-79 0.0010224277768206113 80-81 2.486146569776562E-4 82-83 8.546128833606934E-4 84-85 2.921222219487461E-4 86-87 4.164295504375742E-4 88-89 0.0011467351053094394 90-91 1.67814893459918E-4 92-93 1.6159952703547658E-4 94-95 0.0031822676093139998 96-97 4.941216307430918E-4 98-99 0.0016066722207181037 100 0.0015165494075637031 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.6089156E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.84822533734086 #Duplication Level Percentage of deduplicated Percentage of total 1 94.56307638576928 78.34383061000389 2 4.778307345473678 7.917485673777487 3 0.4585218580362933 1.1396316665006114 4 0.0843007118772673 0.2793665749482436 5 0.03012636412803589 0.124795790193715 6 0.017707793886282325 0.0880235578870743 7 0.010206195021777945 0.059189560150077404 8 0.009237111151321276 0.06122226129045834 9 0.005656759569569478 0.04217872423489664 >10 0.03343192837077572 0.5183565272513535 >50 0.0039680551293239975 0.2356088929103191 >100 0.0038364830030451504 0.5949215893701318 >500 4.246810220082429E-4 0.2391779812116182 >1k 6.516115434263567E-4 1.248104384472606 >5k 2.022100340615022E-4 1.1838790818544724 >10k+ 3.445059839566334E-4 7.924227123943127 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 65582 0.4076161608477163 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53384 0.33180112120238003 No Hit TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50312 0.312707515546496 No Hit TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50210 0.312073548171203 No Hit CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46030 0.28609331651703795 No Hit TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45663 0.283812277039268 No Hit TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43852 0.2725562484446046 No Hit TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42011 0.2611137588572079 No Hit TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40356 0.2508273274247574 No Hit ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39683 0.24664438582110834 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38761 0.24091381797777334 No Hit CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37756 0.23466737472120974 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37139 0.2308324936373294 No Hit TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36461 0.2266184752015581 No Hit TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33047 0.20539921422851518 No Hit TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30990 0.1926142054934392 No Hit GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30650 0.1905009809091291 No Hit CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30565 0.1899726747630516 No Hit CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29520 0.1834776168495103 No Hit ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29039 0.180488025599354 No Hit CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28588 0.17768489534193094 No Hit GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28424 0.17666557524832252 No Hit GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26000 0.1615995270354766 No Hit AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25747 0.16002703933009288 No Hit ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25549 0.1587963967780535 No Hit ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25164 0.15640348070464355 No Hit TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24293 0.1509898965489551 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23005 0.14298450459427456 No Hit TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22332 0.1388015629906255 No Hit CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22181 0.13786304266053484 No Hit TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21400 0.1330088414830461 No Hit TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17779 0.11050299966014376 No Hit ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16883 0.10493403134384427 No Hit AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16422 0.10206874742217677 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0019267635915768359 0.0 0.0 0.0 0.0 2 0.005314138292897403 0.0 0.0 6.215366424441407E-6 0.0 3 0.009248465239568812 0.0 0.0 1.8646099273324218E-5 0.0 4 0.01586783048159891 0.0 0.0 4.3507564971089845E-5 0.0 5 0.025831062859978486 0.0 0.0 4.3507564971089845E-5 0.0 6 0.046677401847554964 0.0 0.0 4.3507564971089845E-5 0.0 7 0.07288138669299993 0.0 0.0 4.3507564971089845E-5 0.0 8 0.21103033620905906 0.0 0.0 4.3507564971089845E-5 0.0 9 0.7417604751921107 0.0 0.0 4.3507564971089845E-5 0.0 10-11 1.7555302465834752 0.0 0.0 4.972293139553125E-5 0.0 12-13 2.729953640824913 0.0 0.0 5.904598103219336E-5 0.0 14-15 3.7071397654420157 0.0 0.0 6.215366424441407E-5 0.0 16-17 4.557190569847169 0.0 0.0 7.147671388107618E-5 0.0 18-19 5.41405341585351 0.0 0.0 8.701512994217969E-5 0.0 20-21 6.51157214213101 0.0 0.0 8.701512994217969E-5 0.0 22-23 7.923075641755229 0.0 0.0 8.701512994217969E-5 0.0 24-25 9.75516055658855 0.0 0.0 8.701512994217969E-5 0.0 26-27 12.113084117028887 0.0 0.0 8.701512994217969E-5 0.0 28-29 15.384812602973081 0.0 0.0 8.701512994217969E-5 0.0 30-31 19.443686169740662 0.0 0.0 8.701512994217969E-5 0.0 32-33 23.801531292256723 0.0 0.0 9.94458627910625E-5 0.0 34-35 27.528019493378025 0.0 0.0 9.94458627910625E-5 0.0 36-37 30.045662432510447 0.0 0.0 1.0255354600328321E-4 0.0 38-39 31.72090879098941 0.0 0.0 1.1809196206438672E-4 0.0 40-41 33.20157067281839 0.0 0.0 1.2741501170104885E-4 0.0 42-43 34.38290983069591 0.0 0.0 1.4295342776215236E-4 0.0 44-45 35.31974890416875 0.0 0.0 1.4295342776215236E-4 0.0 46-47 36.09965929847408 0.0 0.0 1.6159952703547658E-4 0.0 48-49 36.784583355397885 0.0 0.0 1.895686759454629E-4 0.0 50-51 37.44197955442784 0.0 3.1076832122207033E-6 2.1443014164322852E-4 1.2430732848882813E-5 52-53 38.137966963587154 0.0 6.215366424441407E-6 2.6104538982653906E-4 1.2430732848882813E-5 54-55 38.8127040349413 0.0 6.215366424441407E-6 2.952299051609668E-4 1.2430732848882813E-5 56-57 39.488594056767184 0.0 6.215366424441407E-6 3.045529547976289E-4 1.2430732848882813E-5 58-59 40.13341656952049 0.0 6.215366424441407E-6 3.263067372831738E-4 1.2430732848882813E-5 60-61 40.75692037543797 0.0 6.215366424441407E-6 3.3873747013205664E-4 1.2430732848882813E-5 62-63 41.36998858112881 0.0 6.215366424441407E-6 3.4806051976871876E-4 1.2430732848882813E-5 64-65 41.96879873624198 0.0 6.215366424441407E-6 3.573835694053809E-4 1.2430732848882813E-5 66-67 42.51792014447495 0.0 6.215366424441407E-6 4.2264491686201563E-4 1.2430732848882813E-5 68-69 43.019366584549246 0.0 6.215366424441407E-6 4.2264491686201563E-4 1.2430732848882813E-5 70-71 43.498670781736465 0.0 6.215366424441407E-6 4.3507564971089846E-4 1.2430732848882813E-5 72-73 43.96978312597628 0.0 6.215366424441407E-6 4.443986993475606E-4 1.2430732848882813E-5 74-75 44.427560401552455 0.0 6.215366424441407E-6 4.692601650453262E-4 1.2430732848882813E-5 76-77 44.87729188529218 0.0 6.215366424441407E-6 4.910139475308711E-4 1.2430732848882813E-5 78-79 45.30772465628402 0.0 6.215366424441407E-6 5.283061460775195E-4 1.2430732848882813E-5 80-81 45.72599706286644 0.0 6.215366424441407E-6 6.028905431708164E-4 1.2430732848882813E-5 82-83 46.140795079617604 0.0 6.215366424441407E-6 6.215366424441406E-4 1.2430732848882813E-5 84-85 46.55623327911048 0.0 6.215366424441407E-6 6.339673752930235E-4 1.2430732848882813E-5 86-87 46.97088834243387 0.0 6.215366424441407E-6 6.401827417174649E-4 1.2430732848882813E-5 88 47.28224401578305 0.0 6.215366424441407E-6 6.401827417174649E-4 1.2430732848882813E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCCTA 10090 0.0 55.010258 6 GCCCTAC 28520 0.0 49.33865 7 CCCCTAC 69115 0.0 48.55922 7 CCCCCTA 35975 0.0 46.809174 6 GCCTTTT 2220 0.0 45.5471 1 GCCCCTA 7980 0.0 45.52622 6 CCGCCTA 5405 0.0 45.389957 6 CGCCCTA 11595 0.0 43.330322 6 CTCCCTA 27930 0.0 43.161983 6 TGCTCTA 11475 0.0 42.554726 6 TCCCCTA 23785 0.0 41.83143 6 GTCCCTA 3520 0.0 41.791374 6 TTGCCCT 4595 0.0 41.424232 5 ATGCCCT 3640 0.0 40.930103 5 CTGCCCT 5055 0.0 40.8158 5 TGCTCGG 1210 0.0 40.783913 5 CCCGGAA 6490 0.0 40.553497 6 GCCACAC 3025 0.0 40.395496 7 GGCCCTA 4875 0.0 40.394695 6 CCCGCTA 4235 0.0 40.173546 6 >>END_MODULE