##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248490_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16040768
Sequences flagged as poor quality	0
Sequence length	100
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.924160177368066	33.0	31.0	34.0	26.0	34.0
2	31.143466385150635	34.0	31.0	34.0	27.0	34.0
3	31.238339959782476	34.0	31.0	34.0	27.0	34.0
4	34.55921487050994	37.0	35.0	37.0	31.0	37.0
5	34.52197837410279	37.0	35.0	37.0	31.0	37.0
6	34.48802002497636	37.0	35.0	37.0	30.0	37.0
7	34.491585627321584	37.0	35.0	37.0	30.0	37.0
8	34.48621032359548	37.0	35.0	37.0	30.0	37.0
9	35.994753804805356	39.0	37.0	39.0	30.0	39.0
10-11	36.201037662286495	39.0	37.0	39.0	31.0	39.0
12-13	36.24860274769887	39.0	37.0	39.0	31.0	39.0
14-15	37.41882645519217	40.0	38.0	41.0	31.0	41.0
16-17	37.30921324963992	40.0	37.0	41.0	31.0	41.0
18-19	37.12847536352374	40.0	37.0	41.0	30.0	41.0
20-21	37.00059981542031	40.0	36.5	41.0	30.0	41.0
22-23	36.882182667313685	40.0	36.0	41.0	30.0	41.0
24-25	36.7794620868527	40.0	36.0	41.0	29.5	41.0
26-27	36.63538538179718	40.0	35.5	41.0	28.0	41.0
28-29	36.51430408444284	40.0	35.0	41.0	27.5	41.0
30-31	36.43351795874113	39.0	35.0	41.0	27.0	41.0
32-33	36.33069728955621	39.0	35.0	41.0	27.0	41.0
34-35	36.21634908627816	39.0	35.0	41.0	26.0	41.0
36-37	36.09905672845589	39.0	35.0	41.0	26.0	41.0
38-39	35.943950189916094	39.0	35.0	41.0	25.0	41.0
40-41	35.7700383797085	39.0	35.0	40.5	25.0	41.0
42-43	35.54885177567558	38.5	34.5	40.0	25.0	41.0
44-45	35.38039347617271	38.0	34.0	40.0	25.0	41.0
46-47	35.28911100141839	38.0	34.0	40.0	25.0	41.0
48-49	35.28736401523917	38.0	34.0	40.0	25.0	41.0
50-51	35.10252563967012	37.5	34.0	40.0	25.0	41.0
52-53	34.835977117803836	37.0	33.5	40.0	25.0	41.0
54-55	34.717082748157694	37.0	33.0	40.0	25.5	41.0
56-57	34.62031696362668	36.0	33.0	40.0	26.0	41.0
58-59	34.376296540165654	36.0	33.0	39.0	26.0	41.0
60-61	34.115427328666556	35.0	33.0	39.0	26.0	41.0
62-63	33.81759835314618	35.0	33.0	39.0	25.0	41.0
64-65	33.577313411677046	35.0	33.0	38.0	25.0	40.0
66-67	33.34713044911565	35.0	32.5	37.0	25.0	40.0
68-69	33.10621445930768	35.0	32.0	37.0	25.0	40.0
70-71	32.8703323618919	35.0	32.0	36.0	25.0	39.0
72-73	32.6458689197425	35.0	32.0	36.0	25.0	39.0
74-75	32.42140276575286	35.0	32.0	35.5	24.5	39.0
76-77	32.19768822789532	35.0	31.5	35.0	24.0	37.5
78-79	32.020169888374426	35.0	31.0	35.0	24.0	37.0
80-81	31.84104336525533	35.0	31.0	35.0	23.5	37.0
82-83	31.68228316748924	35.0	31.0	35.0	23.0	36.0
84-85	31.532597192353883	35.0	31.0	35.0	23.0	36.0
86-87	31.33955372336287	35.0	31.0	35.0	20.5	36.0
88-89	31.186743084869754	35.0	31.0	35.0	20.0	36.0
90-91	30.982266590976195	35.0	31.0	35.0	19.5	36.0
92-93	30.728390747874414	35.0	30.0	35.0	18.5	35.5
94-95	30.409906900966337	35.0	29.5	35.0	16.0	35.0
96-97	30.038132588165354	35.0	29.0	35.0	13.0	35.0
98-99	29.582069356030836	34.0	28.0	35.0	10.0	35.0
100	28.208629162892947	33.0	25.0	35.0	8.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.46605767803572107
1101	2	0.43648213248919276
1101	3	0.36907923161817635
1101	4	0.3293442367660333
1101	5	0.3122638577853962
1101	6	0.2920166568349387
1101	7	0.31548368786040726
1101	8	0.33591610754950096
1101	9	0.3762056746140132
1101	10-11	0.3992360624306954
1101	12-13	0.36673183498374584
1101	14-15	0.4720942592040558
1101	16-17	0.5045140193991315
1101	18-19	0.4812077317412644
1101	20-21	0.5127869309404005
1101	22-23	0.4927705869624148
1101	24-25	0.5089379797797875
1101	26-27	0.49450738059546495
1101	28-29	0.5141746940055256
1101	30-31	0.48713908278649853
1101	32-33	0.51039159749714
1101	34-35	0.5149689648726294
1101	36-37	0.4947102032795385
1101	38-39	0.4575742009620498
1101	40-41	0.4626210797449417
1101	42-43	0.4757302792296727
1101	44-45	0.5177773992519334
1101	46-47	0.5381844290850637
1101	48-49	0.5228662424997665
1101	50-51	0.5102575781121175
1101	52-53	0.4927328057136009
1101	54-55	0.4694603039550387
1101	56-57	0.427088152330775
1101	58-59	0.4788775055580743
1101	60-61	0.45546921460307743
1101	62-63	0.47352366354900965
1101	64-65	0.42155756001097444
1101	66-67	0.4051914058027535
1101	68-69	0.43671058253441686
1101	70-71	0.45183598248386403
1101	72-73	0.4239669117874456
1101	74-75	0.46638541750643725
1101	76-77	0.44033499791196107
1101	78-79	0.45707586145451273
1101	80-81	0.43719532004650574
1101	82-83	0.4635360457958093
1101	84-85	0.41065537063075297
1101	86-87	0.43704029556742086
1101	88-89	0.40734370377217033
1101	90-91	0.3362420108785926
1101	92-93	0.3208909811289402
1101	94-95	0.339973770891671
1101	96-97	0.32411071049349616
1101	98-99	0.3186512060965043
1101	100	0.38648564416797626
1102	1	0.36516899569621586
1102	2	0.3765373830342007
1102	3	0.34213775850929906
1102	4	0.27972213267690904
1102	5	0.29279246944644655
1102	6	0.2123946112341173
1102	7	0.24632141898426596
1102	8	0.24567118540998933
1102	9	0.2724963236112856
1102	10-11	0.37931303637446945
1102	12-13	0.38393713530264506
1102	14-15	0.5129272487356502
1102	16-17	0.4922136329073723
1102	18-19	0.5450143053206631
1102	20-21	0.5385366258395905
1102	22-23	0.5026715061965419
1102	24-25	0.4926932093234555
1102	26-27	0.5355501156799392
1102	28-29	0.538672912667856
1102	30-31	0.5068239446489784
1102	32-33	0.5476437416774473
1102	34-35	0.5467460440479286
1102	36-37	0.5236491660985649
1102	38-39	0.56040846244737
1102	40-41	0.578662191032528
1102	42-43	0.5535655818243583
1102	44-45	0.5550804067397834
1102	46-47	0.5359742957529932
1102	48-49	0.5128384141867883
1102	50-51	0.48108195502155837
1102	52-53	0.44843522072373787
1102	54-55	0.44225437326220884
1102	56-57	0.47925878761613916
1102	58-59	0.458487732306331
1102	60-61	0.3808523340638885
1102	62-63	0.39081413372664286
1102	64-65	0.39988906867319685
1102	66-67	0.3899247248729125
1102	68-69	0.3749617565465826
1102	70-71	0.37741956050350467
1102	72-73	0.38189440933589935
1102	74-75	0.3849497449179111
1102	76-77	0.3453182557669905
1102	78-79	0.39518977676847555
1102	80-81	0.36347953041252623
1102	82-83	0.3638056557952716
1102	84-85	0.36201883176898875
1102	86-87	0.41252634829390544
1102	88-89	0.34318576346954544
1102	90-91	0.35158740731608873
1102	92-93	0.2765595238591523
1102	94-95	0.29308403437515196
1102	96-97	0.3380155712069346
1102	98-99	0.3447308483539402
1102	100	0.26393538853281484
1103	1	0.37208272112238916
1103	2	0.34904890616017425
1103	3	0.3334513750577628
1103	4	0.29952010561245856
1103	5	0.29426019463732445
1103	6	0.23300756531415345
1103	7	0.2657306835784681
1103	8	0.237515167445153
1103	9	0.26747640013984864
1103	10-11	0.391706226357158
1103	12-13	0.38072085657504573
1103	14-15	0.5210488013276589
1103	16-17	0.5310734033367126
1103	18-19	0.49644905795693717
1103	20-21	0.5164193268493591
1103	22-23	0.5133380135347281
1103	24-25	0.5058259950075907
1103	26-27	0.5238437388668729
1103	28-29	0.5030148994611707
1103	30-31	0.5515582821933549
1103	32-33	0.5669093845625142
1103	34-35	0.5669098200126044
1103	36-37	0.5220602218994372
1103	38-39	0.5678998718186747
1103	40-41	0.5411922514654322
1103	42-43	0.5525445008369374
1103	44-45	0.5874966769684633
1103	46-47	0.5607883235125968
1103	48-49	0.5453825041077849
1103	50-51	0.5429991140964745
1103	52-53	0.5158100506273371
1103	54-55	0.49037884508756235
1103	56-57	0.474390992707022
1103	58-59	0.4580894493234098
1103	60-61	0.42084251074440715
1103	62-63	0.41147198080047076
1103	64-65	0.34606133677560535
1103	66-67	0.38496309180268895
1103	68-69	0.3886896090200054
1103	70-71	0.38042118873898545
1103	72-73	0.3660002561455187
1103	74-75	0.3976885354568651
1103	76-77	0.3625470485073379
1103	78-79	0.3862786655719077
1103	80-81	0.35029336156559765
1103	82-83	0.31076690064099566
1103	84-85	0.2787458444685562
1103	86-87	0.30398411335491815
1103	88-89	0.257331230900963
1103	90-91	0.24130839996654174
1103	92-93	0.19511018773614452
1103	94-95	0.18347977960882034
1103	96-97	0.20770384223305172
1103	98-99	0.16740538314168774
1103	100	0.10136861077806003
1104	1	0.2516921994470742
1104	2	0.2799535345118933
1104	3	0.2774942898808064
1104	4	0.24146264954806895
1104	5	0.1986845978550562
1104	6	0.1455337360450102
1104	7	0.1875856749689504
1104	8	0.2074847244313318
1104	9	0.24640879883261135
1104	10-11	0.2882890660886517
1104	12-13	0.357410291894098
1104	14-15	0.41305839359990415
1104	16-17	0.4513080309524682
1104	18-19	0.44864466420564497
1104	20-21	0.45158564210764496
1104	22-23	0.41664573727016574
1104	24-25	0.40770092935507307
1104	26-27	0.4367481597538756
1104	28-29	0.43977120272307246
1104	30-31	0.42140822300000735
1104	32-33	0.45526363788714974
1104	34-35	0.49317866162320456
1104	36-37	0.44902998388503335
1104	38-39	0.47949570480255943
1104	40-41	0.4803398807393151
1104	42-43	0.4874421810485572
1104	44-45	0.4680644035083432
1104	46-47	0.5043078375464063
1104	48-49	0.4490571944977404
1104	50-51	0.4330278651381718
1104	52-53	0.42160611833966755
1104	54-55	0.389586789892725
1104	56-57	0.39405226356844025
1104	58-59	0.4131678657879405
1104	60-61	0.40620551173800123
1104	62-63	0.4128579077043355
1104	64-65	0.391033190365242
1104	66-67	0.35546522137397574
1104	68-69	0.4065343384450344
1104	70-71	0.41000486277005876
1104	72-73	0.3954348820735021
1104	74-75	0.3627943718575679
1104	76-77	0.3754071547849662
1104	78-79	0.40117369566195293
1104	80-81	0.38306593647441645
1104	82-83	0.36177387691082785
1104	84-85	0.3760027304032114
1104	86-87	0.3726619934675206
1104	88-89	0.35372949411721066
1104	90-91	0.326018887439254
1104	92-93	0.3297605486841384
1104	94-95	0.3388725510310415
1104	96-97	0.3701336942916491
1104	98-99	0.3572128503355003
1104	100	0.33549299659170373
1105	1	0.17650179512079944
1105	2	0.17303053271806235
1105	3	0.196815964799125
1105	4	0.13519271737755645
1105	5	0.13041526628829558
1105	6	0.12772918125509847
1105	7	0.11988527211792643
1105	8	0.09471317931757994
1105	9	0.0916936421769492
1105	10-11	0.15644028096309626
1105	12-13	0.20584625314963034
1105	14-15	0.28953606287358724
1105	16-17	0.26728980859181206
1105	18-19	0.28947303189545437
1105	20-21	0.2575502128063434
1105	22-23	0.2570462418518886
1105	24-25	0.2888201513147237
1105	26-27	0.27542856137223026
1105	28-29	0.30202973212961126
1105	30-31	0.31394296590838877
1105	32-33	0.3052339288262189
1105	34-35	0.33198690865527425
1105	36-37	0.2753237884520132
1105	38-39	0.31121772327313835
1105	40-41	0.31581549932560193
1105	42-43	0.29159240232824146
1105	44-45	0.3268856682823653
1105	46-47	0.3627267645309047
1105	48-49	0.2857897147811954
1105	50-51	0.26325160822453597
1105	52-53	0.20956602961909
1105	54-55	0.2479147072661263
1105	56-57	0.2121887509422251
1105	58-59	0.15192285749104428
1105	60-61	0.16650534721422616
1105	62-63	0.18077406684855646
1105	64-65	0.18909527362364287
1105	66-67	0.15167028229156188
1105	68-69	0.16503601725052874
1105	70-71	0.20546874596184495
1105	72-73	0.20178745921219132
1105	74-75	0.19857015307156445
1105	76-77	0.20515038701756794
1105	78-79	0.24110089627122022
1105	80-81	0.21873143478513413
1105	82-83	0.17342586768447532
1105	84-85	0.17996548751410302
1105	86-87	0.22476382754151558
1105	88-89	0.16498649323230907
1105	90-91	0.1330613842130397
1105	92-93	0.08936718789508902
1105	94-95	0.11189586263210316
1105	96-97	0.16593429173073915
1105	98-99	0.16863792665398591
1105	100	0.16024416196373537
1106	1	0.15059066552057132
1106	2	0.1887396254153373
1106	3	0.2140394312995184
1106	4	0.18013933002978888
1106	5	0.14505188588876194
1106	6	0.10801601216363821
1106	7	0.08326630430843096
1106	8	0.10605354918548215
1106	9	0.14324381850406098
1106	10-11	0.200433581054142
1106	12-13	0.24017691070098124
1106	14-15	0.25810406226708693
1106	16-17	0.27110672106262257
1106	18-19	0.28006062657177466
1106	20-21	0.30375138005693714
1106	22-23	0.26615788127339357
1106	24-25	0.2952866866217647
1106	26-27	0.2937723671305861
1106	28-29	0.30059127631836446
1106	30-31	0.30639922807934994
1106	32-33	0.3052669344675749
1106	34-35	0.33475451354740215
1106	36-37	0.27045860641329256
1106	38-39	0.299594668775093
1106	40-41	0.31686730524479856
1106	42-43	0.3144474494304248
1106	44-45	0.3406083321660063
1106	46-47	0.35742464015720543
1106	48-49	0.32938950206889217
1106	50-51	0.33298584428281686
1106	52-53	0.31343486597928916
1106	54-55	0.28350394102347565
1106	56-57	0.2554454109073774
1106	58-59	0.28812865560141177
1106	60-61	0.2776076400984806
1106	62-63	0.27278819352940076
1106	64-65	0.26044677425073104
1106	66-67	0.25398753426030396
1106	68-69	0.2643677094286261
1106	70-71	0.24870454628867833
1106	72-73	0.26606591702597626
1106	74-75	0.28691357447404897
1106	76-77	0.23912993331991572
1106	78-79	0.253685933183192
1106	80-81	0.2628401629512709
1106	82-83	0.26747996738675184
1106	84-85	0.2555983383747389
1106	86-87	0.2426767466358335
1106	88-89	0.21323847971357068
1106	90-91	0.2557119793132827
1106	92-93	0.21523056133775853
1106	94-95	0.16794557212526584
1106	96-97	0.19483988630543791
1106	98-99	0.15569459359770477
1106	100	0.16516344461320642
1107	1	0.17480508641444814
1107	2	0.1393703012934253
1107	3	0.16755199792327957
1107	4	0.14366591757794112
1107	5	0.15463974001269776
1107	6	0.12805739962330165
1107	7	0.09984499246021272
1107	8	0.12157697292760616
1107	9	0.12457527504004418
1107	10-11	0.09057556153371138
1107	12-13	0.16209892116448543
1107	14-15	0.20054706380586396
1107	16-17	0.18773154040818696
1107	18-19	0.16578148276254012
1107	20-21	0.15498477048700465
1107	22-23	0.14239414739407863
1107	24-25	0.14256737423351495
1107	26-27	0.1691905336240609
1107	28-29	0.22002156704588316
1107	30-31	0.1863329063040311
1107	32-33	0.13600465084433466
1107	34-35	0.1847231674979568
1107	36-37	0.19962622771909366
1107	38-39	0.22706464471547605
1107	40-41	0.20746715032381502
1107	42-43	0.18460214400283803
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2216	74-75	-0.473686131393233
2216	76-77	-0.37654642112514836
2216	78-79	-0.4186938477538824
2216	80-81	-0.37875931214514225
2216	82-83	-0.3591887624209953
2216	84-85	-0.38420579462911064
2216	86-87	-0.3330131118432611
2216	88-89	-0.30486224009036533
2216	90-91	-0.28973681132804074
2216	92-93	-0.32466646548126477
2216	94-95	-0.3007034477602737
2216	96-97	-0.2656285338902542
2216	98-99	-0.2582387527654362
2216	100	-0.30010999936105875
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	12.0
4	41.0
5	448.0
6	3383.0
7	7679.0
8	11132.0
9	14956.0
10	23506.0
11	35405.0
12	45553.0
13	54610.0
14	63059.0
15	71972.0
16	81303.0
17	89988.0
18	98991.0
19	107833.0
20	115507.0
21	123157.0
22	132403.0
23	148137.0
24	195964.0
25	198995.0
26	186720.0
27	257109.0
28	232918.0
29	313941.0
30	346843.0
31	435143.0
32	553841.0
33	788971.0
34	1009901.0
35	1865541.0
36	2473809.0
37	3194764.0
38	2231550.0
39	522345.0
40	3336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.832981313086226	26.779649372871983	28.64049198715471	28.746877326887084
2	14.16811252029759	26.92200345159565	31.94036393805082	26.969520090055937
3	12.669817390198471	28.048515157635922	30.105362838169764	29.176304613995846
4	11.302103039136274	27.257255211028635	30.526438372825826	30.914203377009265
5	10.987291255044127	27.04591143758369	31.691529172036088	30.2752681353361
6	10.906828701683374	24.55661392233032	32.28079688551236	32.25576049047395
7	14.386616087609022	29.16102240616345	27.418896426206672	29.033465080020854
8	13.52186076226986	26.420183676810403	24.23620213900183	35.821753421917904
9	10.777706999460248	25.548888346102732	26.053847794864737	37.619556859572285
10-11	13.510342358652197	42.64276996510285	25.42612911556426	18.420758560680696
12-13	13.050697430720518	43.370297498685446	25.265741663253717	18.31326340734032
14-15	14.051620646201169	41.94867560676919	25.083976526565465	18.915727220464174
16-17	13.86637285283921	41.20753268764032	25.866586935367465	19.059507524153016
18-19	14.062914193816583	39.80695123675546	27.043416427081063	19.08671814234689
20-21	13.163649288010731	39.47456399215856	28.107401891046692	19.25438482878402
22-23	13.445443743009724	40.18421597317729	28.373664444325296	17.996675839487686
24-25	13.773883600601685	40.39531724086228	27.6120319309921	18.218767227543932
26-27	14.958703438341821	40.687288055706155	26.818998887253493	17.53500961869853
28-29	14.683175770072356	41.308028194795874	27.354274558097153	16.654521477034617
30-31	14.907242762220866	41.265970460421165	27.314339086892442	16.512447690465528
32-33	15.262722483978571	41.30312786074655	26.94042901246133	16.49372064281355
34-35	15.454379041299068	41.37832298445289	26.760037591029278	16.40726038321876
36-37	15.953994734235641	41.31368344949027	26.351519479581025	16.380802336693066
38-39	16.30502886297279	41.558360183752164	26.148319303905716	15.988291649369332
40-41	16.47901166018359	41.616975801337	26.169558818683864	15.734453719795546
42-43	16.618566372670088	41.27575670370053	26.52149027038242	15.584186653246961
44-45	16.745377939577935	40.664396647334	26.83515907139744	15.755066341690622
46-47	16.817169762885186	40.23989387510936	27.299359382104004	15.643576979901452
48-49	16.831709124770292	40.343867496701336	27.614672166980508	15.209751211547868
50-51	17.085769899691154	40.55228310987651	27.61084193606286	14.751105054369468
52-53	17.268742353750213	40.93260252898752	27.43258169063358	14.366073426628686
54-55	17.191752523845384	41.38790657693244	27.180055594392233	14.24028530482995
56-57	17.050106534136294	41.54393963052102	26.930983629395183	14.474970205947502
58-59	16.87444227528553	41.316636510903486	27.281877694558915	14.527043519252066
60-61	16.811291139309954	40.871538080845724	27.788154881304294	14.529015898540024
62-63	16.75468819579843	40.2613665358374	28.13711151993199	14.846833748432175
64-65	16.519199127361844	40.07632372965213	28.218943493961145	15.185533649024876
66-67	16.16778428651976	39.65115584195226	28.563156959701995	15.617902911825986
68-69	16.13284012194179	39.37125741502826	28.444750906456584	16.051151556573366
70-71	16.21478271382009	39.263978600112736	28.433792758297404	16.08744592776977
72-73	16.56363457647191	39.32898119017348	28.312475802534404	15.794908430820213
74-75	16.556993286662287	39.430256060572425	28.157141784684853	15.85560886808043
76-77	16.076926098643636	39.88513960516856	28.0197041601274	16.018230136060403
78-79	15.545712655540633	40.53283910712928	27.72410659974614	16.197341637583946
80-81	14.938271493313962	41.402666620606624	27.651406607809374	16.007655278270033
82-83	14.2930828014238	42.28388489491247	27.86544780323676	15.557584500426971
84-85	13.779568396140164	43.308199710121606	27.83433077694662	15.077901116791612
86-87	13.387067226374109	44.32214371558129	27.593059509674433	14.69772954837017
88-89	13.134610182795267	45.45396895709162	27.230704746426547	14.180716113686568
90-91	13.061158966847355	46.52061432608827	26.610811112302425	13.807415594761949
92-93	13.115631102483293	47.1655201967791	25.986937616730028	13.731911084007583
94-95	13.30937189676783	47.453683236900225	25.558870173143127	13.678074693188819
96-97	13.580259269021214	47.29630931454316	25.369552105311367	13.753879311124258
98-99	13.92954172359456	46.80771690338195	25.324778935207704	13.937962437815784
100	14.468353461939174	46.412787947266295	25.106914675083953	14.011943915710576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	415640.0
1	363831.0
2	203119.0
3	74361.0
4	54748.5
5	52924.5
6	46588.0
7	38626.0
8	33600.5
9	33422.0
10	37738.5
11	46708.0
12	61741.5
13	83921.0
14	114667.0
15	154156.5
16	199666.5
17	248424.0
18	299440.5
19	349014.5
20	394390.0
21	433490.0
22	464804.5
23	489946.5
24	508335.5
25	517779.0
26	518478.5
27	509783.0
28	490891.0
29	465715.0
30	436393.0
31	404049.5
32	370511.0
33	337620.5
34	306638.0
35	277050.0
36	249395.0
37	224907.0
38	204074.5
39	187124.0
40	175234.5
41	170196.5
42	174252.5
43	189819.5
44	218173.0
45	258342.5
46	304792.5
47	346883.5
48	372478.0
49	378160.5
50	371157.5
51	360500.0
52	353879.5
53	350454.0
54	339847.5
55	313298.5
56	266293.5
57	205298.5
58	143615.5
59	91072.5
60	53085.5
61	29672.0
62	16959.5
63	10282.0
64	7028.0
65	5372.0
66	4338.5
67	3616.0
68	3022.5
69	2528.5
70	2185.0
71	1912.0
72	1682.5
73	1503.5
74	1370.0
75	1250.5
76	1110.5
77	1050.5
78	1015.0
79	957.0
80	881.0
81	818.0
82	779.5
83	739.5
84	693.5
85	654.5
86	634.0
87	590.5
88	556.5
89	554.5
90	522.0
91	480.5
92	455.5
93	414.5
94	378.0
95	370.5
96	362.5
97	333.5
98	283.5
99	252.5
100	276.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4961877137054786E-4
2	4.426221986378707E-4
3	4.987292379018262E-5
4	7.792644342216033E-4
5	5.04963353375599E-4
6	7.480938568527393E-4
7	8.540738199068773E-4
8	0.0012592913257021112
9	4.613245450591892E-4
10-11	3.77163986163256E-4
12-13	8.883614550126279E-4
14-15	0.0026339137876690193
16-17	0.001402675981598886
18-19	0.003565914050998057
20-21	0.003762288688421901
22-23	0.003915024517529335
24-25	0.005395626942550381
26-27	0.007331319797156844
28-29	0.00932623674876415
30-31	0.009397929076712537
32-33	0.009145447400024737
34-35	0.011439601894373137
36-37	0.01054188926614985
38-39	0.009017648032812395
40-41	0.00737807566321014
42-43	0.0041893255983753395
44-45	0.005090155284335513
46-47	0.007328202739419957
48-49	0.005959814392926822
50-51	0.003784108092580106
52-53	0.003949312152635085
54-55	0.004120750328163838
56-57	0.00669232296109513
58-59	0.007911092536217718
60-61	0.007368724489999481
62-63	0.008824390453125438
64-65	0.009902892430088135
66-67	0.00872464460554507
68-69	0.011243227256949293
70-71	0.011252578430159952
72-73	0.0134438700191911
74-75	0.01772982440740992
76-77	0.018939242809321848
78-79	0.01893612575158496
80-81	0.023243899543961985
82-83	0.021339377266724385
84-85	0.020906106241297177
86-87	0.020834413913348785
88-89	0.02170719007967698
90-91	0.021130534398352996
92-93	0.021791350638572918
94-95	0.019038988656902213
96-97	0.02150146426904248
98-99	0.018303363030997017
100	0.01857142999636925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.6040768E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.80861676047505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.9620873177194	86.18222323452304
2	3.654902406154429	6.5648345898252245
3	0.23886175643230206	0.6435553182648766
4	0.046666997343814756	0.16764393919251067
5	0.02054732465933	0.09226634028914131
6	0.013957836033046317	0.07521203682584436
7	0.010618053478703047	0.06675148869277496
8	0.008375545651065325	0.06017569356291114
9	0.005180281210278537	0.04187105009328555
>10	0.03229528207982003	0.5613138193073264
>50	0.0036557483266730644	0.22763437189185814
>100	0.0021964922441138827	0.3894852178578769
>500	2.5300757814602956E-4	0.15929946090626318
>1k	2.4257216223941755E-4	0.4556553587256612
>5k	7.622470404504567E-5	0.49173442541473444
>10k+	8.315422259459527E-5	3.820343654626601
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	297149	1.8524611789161218	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67006	0.41772314143562206	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56278	0.35084355063298717	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53528	0.33369973308011186	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26890	0.16763536508975133	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21739	0.1355234362843475	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18434	0.11491968464352828	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18362	0.11447082832941664	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16398	0.10222702553892682	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0012904619030709751	0.0	0.0	1.2468230947545654E-5	0.0
2	0.0031544624297290503	0.0	0.0	3.117057736886413E-5	0.0
3	0.005598235695447999	0.0	0.0	4.363880831640979E-5	0.0
4	0.009725220139085609	0.0	0.0	6.85752702115011E-5	0.0
5	0.016794707086343997	0.0	0.0	8.727761663281958E-5	0.0
6	0.028564717100827093	0.0	0.0	1.0597996305413806E-4	0.0
7	0.043264761387983415	0.0	0.0	1.1221407852791088E-4	0.0
8	0.0910554906099259	0.0	0.0	1.1221407852791088E-4	0.0
9	0.26317941884079366	0.0	0.0	1.1844819400168371E-4	0.0
10-11	0.6454740820389647	0.0	0.0	1.1844819400168371E-4	0.0
12-13	1.158385932643624	0.0	0.0	1.2156525173857011E-4	0.0
14-15	1.7533480940563444	0.0	0.0	1.4650171363366144E-4	0.0
16-17	2.3664328291513224	0.0	0.0	1.7143817552875274E-4	0.0
18-19	3.017405401038155	0.0	0.0	2.088428683713897E-4	0.0
20-21	3.830153269469392	0.0	0.0	2.33779330266481E-4	0.0
22-23	4.77165432478046	0.0	0.0	2.4001344574025384E-4	0.0
24-25	5.831176537183257	0.0	0.0	2.4313050347714025E-4	0.0
26-27	7.0611675201586355	0.0	0.0	2.618328498984587E-4	0.0
28-29	8.620297980745061	0.0	0.0	2.743010808460044E-4	0.0
30-31	10.536939378463675	0.0	0.0	2.8365225405666363E-4	0.0
32-33	12.595170006822617	0.0	0.0	2.8676931179355005E-4	0.0
34-35	14.336504960361	0.0	0.0	2.992375427410957E-4	0.0
36-37	15.609149761407933	0.0	0.0	3.1482283142552774E-4	0.0
38-39	16.521571785091588	0.0	0.0	3.8963221711080167E-4	0.0
40-41	17.273530793538065	0.0	0.0	4.363880831640979E-4	0.0
42-43	17.885552611944767	0.0	0.0	4.7379277600673484E-4	0.0
44-45	18.397816114540152	0.0	0.0	5.143145265862582E-4	0.0
46-47	18.841946969122674	0.0	0.0	5.517192194288952E-4	0.0
48-49	19.253426643911315	0.0	0.0	5.95358027745305E-4	0.0
50-51	19.662512418358027	0.0	0.0	6.888697598518974E-4	0.0
52-53	20.089574264773358	0.0	0.0	7.512109145896256E-4	0.0
54-55	20.53501740066311	0.0	0.0	8.260203002748996E-4	0.0
56-57	21.001609773297638	0.0	0.0	8.634249931175366E-4	0.0
58-59	21.479267700898113	0.0	0.0	8.883614550126278E-4	0.0
60-61	21.959285241205407	0.0	0.0	9.413514365396969E-4	0.0
62-63	22.42036977281886	0.0	0.0	0.0010068096490143115	0.0
64-65	22.861876064786923	0.0	0.0	0.0010847360924364719	0.0
66-67	23.275166750120693	0.0	0.0	0.00119071605549061	0.0
68-69	23.656495125420427	0.0	0.0	0.0012530572102283381	0.0
70-71	24.017001555037766	0.0	0.0	0.00131228130722918	0.0
72-73	24.37001769491336	0.0	0.0	0.001349686000071817	0.0
74-75	24.7120773768438	0.0	0.0	0.0013902077506513404	0.0
76-77	25.05124754625215	0.0	0.0	0.0014743683095472734	0.0
78-79	25.38781185539246	0.0	0.0	0.001605284734496503	0.0
80-81	25.721848230708154	0.0	0.0	0.0017517864481301645	0.0
82-83	26.055962532467273	0.0	0.0	0.0018265958338154382	0.0
84-85	26.39421316984324	0.0	0.0	0.0018795858153425073	0.0
86-87	26.73447119240176	0.0	0.0	0.0018951711040269394	3.1170577368864134E-6
88	26.994829673990672	0.0	0.0	0.0019014052195007122	6.234115473772827E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	6235	0.0	46.20319	9
GCTTGCT	1300	0.0	45.185284	7
CTTGCTG	3890	0.0	41.43559	8
GCCTGCT	900	0.0	39.682716	7
CTGCTGA	4485	0.0	39.607487	9
CCTGCTG	3165	0.0	36.97044	8
ACTTGCT	1100	0.0	34.176506	7
CAGTGCC	365	0.0	33.47477	3
CCCGACG	8025	0.0	33.20221	8
CCTGGCC	685	0.0	31.557627	3
TGCCAAA	2245	0.0	31.398138	6
TGCTTGC	750	0.0	31.328365	6
GGCTTGC	330	0.0	31.328363	6
CATTGCC	665	0.0	31.093395	3
GTTGCTG	1825	0.0	30.89921	8
CATGCTG	740	0.0	29.846617	8
GTGCTCC	505	0.0	29.778011	3
TCGCGAC	715	0.0	29.57573	6
TGACGAC	890	0.0	29.568344	6
ACTTGCC	1005	0.0	29.458565	3
>>END_MODULE