##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248490_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16040768 Sequences flagged as poor quality 0 Sequence length 100 %GC 33 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44413291184063 34.0 31.0 34.0 31.0 34.0 2 32.70967568385753 34.0 31.0 34.0 31.0 34.0 3 32.817490721142526 34.0 31.0 34.0 31.0 34.0 4 36.24968935402594 37.0 37.0 37.0 35.0 37.0 5 36.16122744247657 37.0 35.0 37.0 35.0 37.0 6 36.1643266706432 37.0 37.0 37.0 35.0 37.0 7 36.1621956629508 37.0 37.0 37.0 35.0 37.0 8 36.147188900182336 37.0 36.0 37.0 35.0 37.0 9 37.933038929308125 39.0 38.0 39.0 35.0 39.0 10-11 37.937576835473216 39.0 38.0 39.0 35.0 39.0 12-13 37.90480493203318 39.0 38.0 39.0 35.0 39.0 14-15 39.31525610245095 41.0 39.0 41.0 36.0 41.0 16-17 39.26533642279472 41.0 39.0 41.0 36.0 41.0 18-19 39.15910818609184 41.0 39.0 41.0 36.0 41.0 20-21 39.015863236722836 40.5 39.0 41.0 35.0 41.0 22-23 38.85479195883888 40.0 38.0 41.0 35.0 41.0 24-25 38.705957189830315 40.0 38.0 41.0 35.0 41.0 26-27 38.55287019299824 40.0 38.0 41.0 35.0 41.0 28-29 38.43834918003926 40.0 38.0 41.0 34.0 41.0 30-31 38.36120530513252 40.0 38.0 41.0 34.0 41.0 32-33 38.27096832271373 40.0 38.0 41.0 34.0 41.0 34-35 38.24227858042707 40.0 38.0 41.0 34.0 41.0 36-37 38.152628789344746 40.0 37.0 41.0 33.5 41.0 38-39 38.10998379254659 40.0 37.0 41.0 33.5 41.0 40-41 37.99313810909802 40.0 37.0 41.0 33.0 41.0 42-43 37.848997005629656 40.0 36.5 41.0 33.0 41.0 44-45 37.64963186301304 40.0 36.0 41.0 33.0 41.0 46-47 37.422395610983216 39.5 35.5 41.0 33.0 41.0 48-49 37.16176921828182 39.0 35.0 41.0 32.0 41.0 50-51 36.861978460133585 39.0 35.0 41.0 32.0 41.0 52-53 36.55957083850349 38.0 35.0 40.5 31.0 41.0 54-55 36.330186621987174 37.0 35.0 40.0 31.0 41.0 56-57 36.08389361407134 37.0 35.0 40.0 31.0 41.0 58-59 35.79792725635082 36.0 35.0 40.0 31.0 41.0 60-61 35.511155357399346 35.5 35.0 39.0 31.0 41.0 62-63 35.23574569496922 35.0 35.0 39.0 30.5 41.0 64-65 34.97572273347511 35.0 34.5 39.0 30.0 41.0 66-67 34.718894818502456 35.0 34.0 37.5 30.0 40.0 68-69 34.46570204743314 35.0 34.0 37.0 30.0 40.0 70-71 34.25428389713011 35.0 34.0 37.0 29.0 39.5 72-73 34.040951655182596 35.0 34.0 36.0 29.0 39.0 74-75 33.82375139394822 35.0 34.0 36.0 29.0 39.0 76-77 33.617405039459456 35.0 34.0 35.5 29.0 38.5 78-79 33.404145611980674 35.0 33.0 35.0 29.0 37.0 80-81 33.21061195448996 35.0 33.0 35.0 29.0 37.0 82-83 33.082016895949124 35.0 33.0 35.0 29.0 36.0 84-85 32.96910472117046 35.0 33.0 35.0 29.0 36.0 86-87 32.872220457274864 35.0 33.0 35.0 29.0 36.0 88-89 32.768854895226966 35.0 33.0 35.0 28.0 36.0 90-91 32.69807249877313 35.0 33.0 35.0 27.0 35.5 92-93 32.60479139776849 35.0 33.0 35.0 27.0 35.0 94-95 32.467577830438046 35.0 33.0 35.0 27.0 35.0 96-97 32.359559997376685 35.0 33.0 35.0 27.0 35.0 98-99 32.239052924398635 35.0 33.0 35.0 25.5 35.0 100 30.978763298615128 34.0 31.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2775979670663631 1101 2 0.2675226487994493 1101 3 0.25992887242616547 1101 4 0.15370310484074423 1101 5 0.17913940654435834 1101 6 0.1831472165036061 1101 7 0.20301273345553028 1101 8 0.19654602747039718 1101 9 0.261103903627081 1101 10-11 0.26034086697625725 1101 12-13 0.2651152112294355 1101 14-15 0.378459564602295 1101 16-17 0.3629319786808409 1101 18-19 0.3761471476625289 1101 20-21 0.3712146034533035 1101 22-23 0.3534286579647983 1101 24-25 0.3557096561884876 1101 26-27 0.3498923154476117 1101 28-29 0.3863374105605999 1101 30-31 0.37605246358916844 1101 32-33 0.3639677192153741 1101 34-35 0.4034178707400713 1101 36-37 0.4465007172176243 1101 38-39 0.4215303393950478 1101 40-41 0.40839116769608097 1101 42-43 0.41590233344974337 1101 44-45 0.41074045549432014 1101 46-47 0.3987168157519889 1101 48-49 0.37645806653884506 1101 50-51 0.38561905867170054 1101 52-53 0.37410390681741745 1101 54-55 0.39598798895067944 1101 56-57 0.37488236795095986 1101 58-59 0.38685853554614624 1101 60-61 0.3832502234878419 1101 62-63 0.3881298711522021 1101 64-65 0.37103843008176796 1101 66-67 0.3776182210438108 1101 68-69 0.38820348581588604 1101 70-71 0.3839524838412629 1101 72-73 0.3776963757603511 1101 74-75 0.34413688379637364 1101 76-77 0.3800817756888364 1101 78-79 0.3603283287331891 1101 80-81 0.4118256284846282 1101 82-83 0.3603266814142998 1101 84-85 0.3229676128542849 1101 86-87 0.3265391145155263 1101 88-89 0.34834497448122903 1101 90-91 0.38425013923009743 1101 92-93 0.3653951688577308 1101 94-95 0.3728960847305558 1101 96-97 0.40451046691158865 1101 98-99 0.4358483148664405 1101 100 0.38740298552670893 1102 1 0.20638865149911823 1102 2 0.22392292981730577 1102 3 0.21598147297682146 1102 4 0.1502406807387544 1102 5 0.17405263668804594 1102 6 0.15964449554169136 1102 7 0.16939636181727025 1102 8 0.1604405833528162 1102 9 0.24682743142160746 1102 10-11 0.2510735358953511 1102 12-13 0.2499886497432371 1102 14-15 0.3368631095133452 1102 16-17 0.3122993706018775 1102 18-19 0.3183749927632249 1102 20-21 0.2936811852481185 1102 22-23 0.30830362156548574 1102 24-25 0.33681483429845827 1102 26-27 0.34617805353512665 1102 28-29 0.3614846768182076 1102 30-31 0.36102845861966415 1102 32-33 0.37586218722482556 1102 34-35 0.3945311154057265 1102 36-37 0.43440372687288686 1102 38-39 0.3812184292673635 1102 40-41 0.3889328360364601 1102 42-43 0.3616180837051175 1102 44-45 0.3436444330168342 1102 46-47 0.36438017515394705 1102 48-49 0.3801778106319702 1102 50-51 0.32631477589294633 1102 52-53 0.30758138586126194 1102 54-55 0.3055500378475742 1102 56-57 0.27698635686901696 1102 58-59 0.2891994306325216 1102 60-61 0.3321591101461365 1102 62-63 0.3081510512947574 1102 64-65 0.293915356500726 1102 66-67 0.3436396742020307 1102 68-69 0.3572899038817354 1102 70-71 0.3369282835067793 1102 72-73 0.294455932858817 1102 74-75 0.29197491417660615 1102 76-77 0.2932579867537015 1102 78-79 0.3467463348683495 1102 80-81 0.35072046583103145 1102 82-83 0.3352781062639991 1102 84-85 0.25478865002894224 1102 86-87 0.23278957063207883 1102 88-89 0.27681455505739194 1102 90-91 0.3155967054160769 1102 92-93 0.33115779371158993 1102 94-95 0.3258963423425456 1102 96-97 0.30914961419358633 1102 98-99 0.27940835796582064 1102 100 0.2949389811691354 1103 1 0.20505947602253372 1103 2 0.2071027851151257 1103 3 0.21060839100943696 1103 4 0.13220737409985617 1103 5 0.12205638502570793 1103 6 0.15014958772857057 1103 7 0.14352967197736888 1103 8 0.16403172671217447 1103 9 0.20556383366830744 1103 10-11 0.20539451011477894 1103 12-13 0.2256452518008345 1103 14-15 0.29747774527667303 1103 16-17 0.30515748257231934 1103 18-19 0.3117691824711315 1103 20-21 0.28585257581200096 1103 22-23 0.28048516262588663 1103 24-25 0.29461581637847445 1103 26-27 0.3062460963479978 1103 28-29 0.28589717985608587 1103 30-31 0.2954331668857364 1103 32-33 0.28672981125443187 1103 34-35 0.33699977408556947 1103 36-37 0.3360728336167611 1103 38-39 0.3295105889168397 1103 40-41 0.31434070554844595 1103 42-43 0.29391643964718384 1103 44-45 0.32657284917574714 1103 46-47 0.31202746361086753 1103 48-49 0.31731637625464515 1103 50-51 0.2733483696584784 1103 52-53 0.27404989616857023 1103 54-55 0.2655676693696307 1103 56-57 0.3080577747357509 1103 58-59 0.28047143263121654 1103 60-61 0.29194111288820324 1103 62-63 0.2870943435477926 1103 64-65 0.306651185595328 1103 66-67 0.2896652903286494 1103 68-69 0.2589964207585993 1103 70-71 0.2550000171802651 1103 72-73 0.2722366871894124 1103 74-75 0.25352970216100346 1103 76-77 0.22800608700590885 1103 78-79 0.27733344521599435 1103 80-81 0.30521072622032364 1103 82-83 0.2721829471902737 1103 84-85 0.26534530655125366 1103 86-87 0.23966804444280143 1103 88-89 0.23428114418816648 1103 90-91 0.231393378285901 1103 92-93 0.26201797634061563 1103 94-95 0.2293608706083603 1103 96-97 0.22684611201201932 1103 98-99 0.21484477505060084 1103 100 0.23265987311379988 1104 1 0.15478538807358433 1104 2 0.1703020001247637 1104 3 0.1569707173002044 1104 4 0.10330879351536737 1104 5 0.11745558069535633 1104 6 0.12417405434251805 1104 7 0.13294863153769398 1104 8 0.12595199078909758 1104 9 0.16830035399664922 1104 10-11 0.16876338260644985 1104 12-13 0.19434561239405213 1104 14-15 0.2472944053636681 1104 16-17 0.23739870411472452 1104 18-19 0.23501281891993386 1104 20-21 0.2526116980529167 1104 22-23 0.22368461594813027 1104 24-25 0.20789599248769974 1104 26-27 0.2434575838091959 1104 28-29 0.24482978920581644 1104 30-31 0.23277126977417595 1104 32-33 0.22630657311356117 1104 34-35 0.27176206413006554 1104 36-37 0.2965733202849279 1104 38-39 0.30691936326796565 1104 40-41 0.2687392080729154 1104 42-43 0.2917452821705737 1104 44-45 0.3064255960027822 1104 46-47 0.2680773239822756 1104 48-49 0.28668596385799106 1104 50-51 0.24051511037114892 1104 52-53 0.23490549904898472 1104 54-55 0.22371095708711408 1104 56-57 0.233055809999982 1104 58-59 0.2495939151046116 1104 60-61 0.23740364116154922 1104 62-63 0.19168410577075434 1104 64-65 0.1744207584447821 1104 66-67 0.22104996135033872 1104 68-69 0.22783940939534375 1104 70-71 0.22465568856449636 1104 72-73 0.21889802627335087 1104 74-75 0.23146078885434918 1104 76-77 0.22836500981964036 1104 78-79 0.22241398982162508 1104 80-81 0.2572034131521903 1104 82-83 0.26939606649233383 1104 84-85 0.2518632268773757 1104 86-87 0.2454003892118095 1104 88-89 0.23244201046335178 1104 90-91 0.24602151365928648 1104 92-93 0.22236801632039516 1104 94-95 0.23386639730381376 1104 96-97 0.2352994321104589 1104 98-99 0.23927274221131256 1104 100 0.25604716639598024 1105 1 0.10754532124192195 1105 2 0.12652435664809047 1105 3 0.1292992599671834 1105 4 0.09359995356613382 1105 5 0.10696300377173174 1105 6 0.09207059803819106 1105 7 0.12520637216585584 1105 8 0.08945375780499631 1105 9 0.1238619054349428 1105 10-11 0.13497916279375488 1105 12-13 0.14475348777384767 1105 14-15 0.18354446757133047 1105 16-17 0.18798222733346392 1105 18-19 0.18071289002707402 1105 20-21 0.1577561500990754 1105 22-23 0.16235159503424512 1105 24-25 0.13847614313607437 1105 26-27 0.1418428507931182 1105 28-29 0.15690274539736748 1105 30-31 0.17994264445541575 1105 32-33 0.17341475970103204 1105 34-35 0.2243265362195359 1105 36-37 0.22150732559970265 1105 38-39 0.23011430492849172 1105 40-41 0.216960249670052 1105 42-43 0.21590136489547262 1105 44-45 0.2140121662772927 1105 46-47 0.2357156135197087 1105 48-49 0.24093319726243578 1105 50-51 0.22266927515624246 1105 52-53 0.209064097930316 1105 54-55 0.1947328120342391 1105 56-57 0.1930519521586973 1105 58-59 0.2190816819237682 1105 60-61 0.17762251812528973 1105 62-63 0.17513687611069884 1105 64-65 0.2175391882613127 1105 66-67 0.2175948301315529 1105 68-69 0.21412172467029933 1105 70-71 0.20927460959705968 1105 72-73 0.194033325239225 1105 74-75 0.189791430974509 1105 76-77 0.20021889296116768 1105 78-79 0.21412603369567051 1105 80-81 0.21877882853852526 1105 82-83 0.22337416437082425 1105 84-85 0.22215752214885498 1105 86-87 0.21975087983332742 1105 88-89 0.2204017699890457 1105 90-91 0.24191218176878948 1105 92-93 0.24061724932748518 1105 94-95 0.24958038814979489 1105 96-97 0.25350905284685865 1105 98-99 0.2440158306473208 1105 100 0.22614682756115911 1106 1 0.1319071440542956 1106 2 0.11899600016850798 1106 3 0.1166175886303904 1106 4 0.07109642908628899 1106 5 0.09699881910211161 1106 6 0.09519159360720408 1106 7 0.08876247974125562 1106 8 0.07085882549800715 1106 9 0.10859552706929065 1106 10-11 0.10461439232631875 1106 12-13 0.1257384630203049 1106 14-15 0.15563202400999643 1106 16-17 0.13228290667758813 1106 18-19 0.15467976770892022 1106 20-21 0.1318490776797887 1106 22-23 0.14562454031211303 1106 24-25 0.11971144681215407 1106 26-27 0.12160322689977221 1106 28-29 0.12586285564118782 1106 30-31 0.1342983736337473 1106 32-33 0.1319791747101675 1106 34-35 0.16555084168803091 1106 36-37 0.1946610349447937 1106 38-39 0.17921946092375407 1106 40-41 0.17182823716547801 1106 42-43 0.1449511831316741 1106 44-45 0.17571826501217913 1106 46-47 0.18610061125981048 1106 48-49 0.19058499180510324 1106 50-51 0.17271122584175913 1106 52-53 0.13996057017080688 1106 54-55 0.1579054430387572 1106 56-57 0.1651538400782755 1106 58-59 0.15079175485347207 1106 60-61 0.11679685812242013 1106 62-63 0.15387385659833086 1106 64-65 0.10628525550255574 1106 66-67 0.1366161097665426 1106 68-69 0.1732158799952117 1106 70-71 0.1307371090580034 1106 72-73 0.16218147025367813 1106 74-75 0.14395150515539257 1106 76-77 0.12946254830775672 1106 78-79 0.16057021623424106 1106 80-81 0.1361365554388314 1106 82-83 0.09005014777707743 1106 84-85 0.12743510860458684 1106 86-87 0.11842093026187683 1106 88-89 0.10124310880717502 1106 90-91 0.1378413346474403 1106 92-93 0.14622871860734676 1106 94-95 0.1382802629994444 1106 96-97 0.14775104599888067 1106 98-99 0.1569319395451032 1106 100 0.08018284522519892 1107 1 0.11386128234397574 1107 2 0.0965990764340674 1107 3 0.08659784103754475 1107 4 0.05380173286268786 1107 5 0.033516552697754776 1107 6 0.035537698704175114 1107 7 0.06488551741269788 1107 8 0.07010182470476423 1107 9 0.0867317384775319 1107 10-11 0.1005351289531049 1107 12-13 0.09631992388454336 1107 14-15 0.1097071399658418 1107 16-17 0.1191457690130946 1107 18-19 0.12407384279124045 1107 20-21 0.12081863037103346 1107 22-23 0.11958995892457835 1107 24-25 0.10207932534780895 1107 26-27 0.10789107476237092 1107 28-29 0.10620057455903975 1107 30-31 0.14656115454541663 1107 32-33 0.10307090568144872 1107 34-35 0.15597992959698104 1107 36-37 0.1404804304327527 1107 38-39 0.13136903096049934 1107 40-41 0.12316371792318392 1107 42-43 0.10955855995149477 1107 44-45 0.12421226719666123 1107 46-47 0.13929032249293272 1107 48-49 0.15526838020123535 1107 50-51 0.15726024707064568 1107 52-53 0.09959006132030623 1107 54-55 0.11616499293752014 1107 56-57 0.11991916475476216 1107 58-59 0.11696074266107814 1107 60-61 0.0977552751054489 1107 62-63 0.09973623551192645 1107 64-65 0.10230657969454171 1107 66-67 0.1123595341763135 1107 68-69 0.12159068986125732 1107 70-71 0.11672142890007109 1107 72-73 0.11723316430815345 1107 74-75 0.11415131382082677 1107 76-77 0.08669000489230427 1107 78-79 0.13089091238607153 1107 80-81 0.16261670115388682 1107 82-83 0.10395156239026448 1107 84-85 0.1029736483338013 1107 86-87 0.08380208065413086 1107 88-89 0.09385716471850003 1107 90-91 0.10326218511882956 1107 92-93 0.09555592960090564 1107 94-95 0.08188596558791517 1107 96-97 0.09387205860672765 1107 98-99 0.07029364127286186 1107 100 0.027759972021183188 1108 1 0.062474585261071525 1108 2 0.062005393977024426 1108 3 0.0700513758277026 1108 4 0.02239454888735537 1108 5 0.033258726261472304 1108 6 0.019628473426877235 1108 7 0.025439495447471927 1108 8 0.02496151193359708 1108 9 0.04167163313101696 1108 10-11 0.05723645444305703 1108 12-13 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2214 76-77 -0.29354593132366347 2214 78-79 -0.28025672470459284 2214 80-81 -0.2756018208689923 2214 82-83 -0.28863497245187375 2214 84-85 -0.28218535112608123 2214 86-87 -0.2632907587597444 2214 88-89 -0.27344488563642955 2214 90-91 -0.2834814511279333 2214 92-93 -0.28963082762768266 2214 94-95 -0.26688739096345415 2214 96-97 -0.24871234027222755 2214 98-99 -0.22596719666965015 2214 100 -0.31105632667684446 2215 1 -0.2780259792993931 2215 2 -0.31523286891297886 2215 3 -0.27832136033674715 2215 4 -0.16399991845162987 2215 5 -0.2040797179599636 2215 6 -0.24581761062985663 2215 7 -0.26664092389778915 2215 8 -0.27878120294872133 2215 9 -0.37183293580024923 2215 10-11 -0.34862879722619056 2215 12-13 -0.3171391770041154 2215 14-15 -0.4157747791358233 2215 16-17 -0.39714927620222085 2215 18-19 -0.362667383409196 2215 20-21 -0.38745118041671844 2215 22-23 -0.3999737464929183 2215 24-25 -0.41161810177461433 2215 26-27 -0.46935453201786004 2215 28-29 -0.46775621415209656 2215 30-31 -0.49143443276687293 2215 32-33 -0.48768972489843065 2215 34-35 -0.4752719475301461 2215 36-37 -0.4585822462468556 2215 38-39 -0.504818800574597 2215 40-41 -0.4837823107367001 2215 42-43 -0.5234415538006303 2215 44-45 -0.5381242262121546 2215 46-47 -0.5236715614489142 2215 48-49 -0.48476468051438815 2215 50-51 -0.46978177561882006 2215 52-53 -0.4454154374909862 2215 54-55 -0.44924997362847563 2215 56-57 -0.5306353998658295 2215 58-59 -0.5387624825836355 2215 60-61 -0.5246355729819854 2215 62-63 -0.449131376717844 2215 64-65 -0.4843901968437905 2215 66-67 -0.5550963469903039 2215 68-69 -0.5382382836698909 2215 70-71 -0.4452103813484598 2215 72-73 -0.40413058787905953 2215 74-75 -0.39747727697817226 2215 76-77 -0.3842892658370545 2215 78-79 -0.3460049136191188 2215 80-81 -0.33696490704068793 2215 82-83 -0.38130647236381776 2215 84-85 -0.373815927250007 2215 86-87 -0.34867285059286246 2215 88-89 -0.30981297368349203 2215 90-91 -0.37095909467254984 2215 92-93 -0.37891408120901815 2215 94-95 -0.3646744128249324 2215 96-97 -0.3668855756525389 2215 98-99 -0.33369156016222945 2215 100 -0.4842722403840227 2216 1 -0.3091874699387205 2216 2 -0.3207086790552367 2216 3 -0.33325484331838595 2216 4 -0.21290934008762719 2216 5 -0.2568313579504533 2216 6 -0.27759358208449214 2216 7 -0.2722815103684155 2216 8 -0.27831403659730825 2216 9 -0.38558618550546697 2216 10-11 -0.3587829931665354 2216 12-13 -0.3799835623343597 2216 14-15 -0.49825211074902853 2216 16-17 -0.517653051675758 2216 18-19 -0.5265500030744974 2216 20-21 -0.4839190287320818 2216 22-23 -0.47684767531858085 2216 24-25 -0.4698605804825391 2216 26-27 -0.516076182481477 2216 28-29 -0.5099754295636956 2216 30-31 -0.5327215640340839 2216 32-33 -0.5442198858837344 2216 34-35 -0.5591496914043361 2216 36-37 -0.5663857163246533 2216 38-39 -0.576518618339648 2216 40-41 -0.5803938208830601 2216 42-43 -0.5848463123469116 2216 44-45 -0.6571815734473319 2216 46-47 -0.6245328098253466 2216 48-49 -0.5934373482733974 2216 50-51 -0.5722255706879125 2216 52-53 -0.5563634934295365 2216 54-55 -0.49373445180929565 2216 56-57 -0.450688495977289 2216 58-59 -0.3723336874876395 2216 60-61 -0.43997224913058375 2216 62-63 -0.42620910674585843 2216 64-65 -0.44298114140973155 2216 66-67 -0.41045851628546615 2216 68-69 -0.4898002028229129 2216 70-71 -0.4375722970909095 2216 72-73 -0.4388657341891147 2216 74-75 -0.4035385933801905 2216 76-77 -0.4569747223023697 2216 78-79 -0.39438139812005346 2216 80-81 -0.3905233416682776 2216 82-83 -0.4119089530264901 2216 84-85 -0.4394595710883351 2216 86-87 -0.42088137156311234 2216 88-89 -0.4516532654366685 2216 90-91 -0.4958511883091816 2216 92-93 -0.4984279703720489 2216 94-95 -0.5350676893154542 2216 96-97 -0.4951664411572594 2216 98-99 -0.5220660635326553 2216 100 -0.5331106449999048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 72.0 7 190.0 8 416.0 9 684.0 10 999.0 11 1390.0 12 2030.0 13 2866.0 14 4195.0 15 6384.0 16 9756.0 17 14786.0 18 20662.0 19 28057.0 20 36141.0 21 46202.0 22 57923.0 23 71434.0 24 87930.0 25 106635.0 26 128319.0 27 154890.0 28 186610.0 29 227456.0 30 271384.0 31 333464.0 32 422842.0 33 566660.0 34 800887.0 35 1863357.0 36 2337531.0 37 4511432.0 38 2990231.0 39 742193.0 40 4759.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.08586465176726 23.08646374200345 33.234325001088514 24.593346605140773 2 16.449902620302222 24.042177357547864 38.30977598149502 21.198144040654892 3 14.363501797420172 21.998641212191338 40.062807466575165 23.575049523813323 4 14.402970880382465 20.28972180694352 42.3897809874054 22.91752632526861 5 13.214484717174816 20.699956348500308 44.18181990470981 21.903739029615068 6 12.559049541767575 16.776590746777213 41.58329576239741 29.0810639490578 7 16.90770042930613 18.546587046206266 38.4211466682892 26.124565856198405 8 12.000946588093537 18.406263341007115 40.36989376069774 29.222896310201605 9 13.847865638353477 17.234050140242662 40.383459195968676 28.53462502543519 10-11 17.84699460773948 28.533437426437438 38.033808605672746 15.585759360150336 12-13 15.928087109046151 32.880919417324655 35.48370314937539 15.707290324253801 14-15 16.70199020396031 30.7234541388542 35.63432249627948 16.94023316090601 16-17 20.3011391450809 30.386940891039593 36.17001254460707 13.141907419272439 18-19 20.266164936741184 26.863180740473275 36.30845480715138 16.562199515634163 20-21 16.83159033458999 26.444436859525695 40.14721080458168 16.57676200130263 22-23 16.743260048396685 27.474569796159386 40.82837866615863 14.953791489285301 24-25 17.099277091609125 28.44833100177298 39.25137898703054 15.20101291958735 26-27 19.333142278474448 28.504785431719977 36.775449280233964 15.386623009571613 28-29 17.92322025499155 30.562852761342395 38.43134184294305 13.08258514072301 30-31 17.841734978720105 29.272607158017216 38.49015384402465 14.39550401923803 32-33 18.194854080284674 29.7796818960804 38.365788336771466 13.659675686863453 34-35 18.290205620317778 29.822277415055297 38.2479163978198 13.639600566807122 36-37 18.623450572940147 29.346384786563835 37.961636250832875 14.068528389663138 38-39 18.98304251062896 29.62636767860215 37.42266833936698 13.967921471401908 40-41 19.150789031110705 29.890334297980726 37.34739256876347 13.611484102145106 42-43 19.221576760795227 29.72878214140125 37.57347939659321 13.476161701210312 44-45 19.258995301871355 29.005607188467547 37.767000087308894 13.968397422352204 46-47 19.302387480476664 28.72200678291713 38.03679300675589 13.93881272985031 48-49 18.796908671719134 28.918535392286266 38.0792955908502 14.2052603451444 50-51 18.736861391670008 29.01898380745862 38.00403154478536 14.240123256086006 52-53 18.656766058227273 29.30781044420263 37.917960895256556 14.117462602313543 54-55 18.631070131607263 29.51344687245414 37.95749090628895 13.897992089649641 56-57 18.520173563000125 29.39231753934636 37.74666402672295 14.340844870930566 58-59 18.537629200099058 29.321411833941234 37.827136388579916 14.313822577379792 60-61 18.377337112247623 29.067477309760058 37.83685470064157 14.718330877350748 62-63 18.196659910547925 28.954096836261208 37.87556805260197 14.973675200588898 64-65 18.17618707340128 28.85508715686053 37.800060938499655 15.16866483123854 66-67 17.87875742607835 28.536856838774803 38.36166073843846 15.222724996708386 68-69 17.69245951704417 28.16552117876188 38.86658321677092 15.275436087423023 70-71 17.437775011117882 27.990521427765664 39.29793405814614 15.273769502970321 72-73 17.504125001815705 27.612098525747076 39.57118434292884 15.312592129508381 74-75 17.706892761262985 27.355132043796416 39.61979386273789 15.31818133220271 76-77 18.15319194511579 27.360743436467317 39.49005009241462 14.996014526002272 78-79 18.224157864271415 27.477942709802583 39.62325322314637 14.674646202779632 80-81 17.7826755173844 27.33679626735262 40.21773506235656 14.662793152906417 82-83 17.488781004662403 27.146309264735624 40.63956689541048 14.725342835191487 84-85 17.396381102825494 27.122312592602242 40.641260671944465 14.840045632627795 86-87 17.470568859954444 27.344056032946927 40.49529804035116 14.69007706674747 88-89 17.525042222524096 27.77222485754749 40.173907985663135 14.528824934265277 90-91 17.511878423839892 27.896686958431822 40.02648006933095 14.564954548397335 92-93 17.29639547200469 27.89539149095419 39.96784811873716 14.840364918303962 94-95 17.07987869532387 28.173836453646175 40.042232450921034 14.704052400108925 96-97 16.80344476195618 28.664141382340997 40.22341104522732 14.309002810475501 98-99 16.689286988402962 29.264956694819855 40.03557241840857 14.010183898368616 100 16.659978408469687 29.90805364579479 39.68506938260031 13.746898563135215 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 138354.0 1 290753.0 2 397193.5 3 237336.0 4 79472.5 5 28009.5 6 18551.5 7 16684.5 8 17755.5 9 21168.0 10 27690.0 11 38217.0 12 54995.0 13 79507.5 14 111909.0 15 153323.0 16 200599.0 17 250905.0 18 302765.0 19 352344.0 20 395618.5 21 432015.0 22 460911.5 23 482347.5 24 497366.5 25 505031.0 26 504524.0 27 495406.5 28 478230.0 29 454066.5 30 426117.0 31 395574.5 32 363170.5 33 331296.0 34 300410.5 35 270733.0 36 242404.0 37 214191.0 38 187861.5 39 164325.0 40 142736.0 41 123495.0 42 107447.5 43 94447.0 44 85722.0 45 83856.0 46 91286.0 47 110519.0 48 141432.5 49 185150.0 50 238369.5 51 302640.5 52 384523.0 53 479451.5 54 569073.5 55 620029.5 56 603177.5 57 512194.0 58 372584.5 59 230322.5 60 120382.5 61 53490.0 62 21172.5 63 8171.0 64 3497.5 65 1722.5 66 988.0 67 704.0 68 529.5 69 384.5 70 305.0 71 245.5 72 182.0 73 141.0 74 119.5 75 85.5 76 62.0 77 43.5 78 26.5 79 21.5 80 15.5 81 12.0 82 9.0 83 6.5 84 3.5 85 4.0 86 5.0 87 4.0 88 3.5 89 4.0 90 2.5 91 1.0 92 2.0 93 2.0 94 1.0 95 0.0 96 1.0 97 1.0 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06105692695013106 2 0.0060907308178760515 3 0.0 4 6.234115473772828E-6 5 5.111974688493718E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.545821247461468E-5 18-19 0.0 20-21 0.0010317461109094028 22-23 0.0 24-25 4.987292379018262E-5 26-27 0.0 28-29 6.234115473772828E-6 30-31 2.555987344246859E-4 32-33 6.10943316429737E-4 34-35 2.3689638800336742E-4 36-37 0.0 38-39 2.4001344574025384E-4 40-41 1.1844819400168371E-4 42-43 2.930034272673229E-4 44-45 1.2156525173857011E-4 46-47 1.839064064762984E-4 48-49 5.641874503764407E-4 50-51 1.153311362647973E-4 52-53 1.8702346421318482E-5 54-55 7.480938568527393E-5 56-57 1.371505404230022E-4 58-59 3.117057736886414E-6 60-61 3.428763510575055E-5 62-63 0.0 64-65 3.428763510575055E-5 66-67 0.0 68-69 3.117057736886413E-5 70-71 0.0010379802263831756 72-73 0.0010535655150676077 74-75 3.023546004779821E-4 76-77 9.974584758036524E-5 78-79 9.538196674872425E-4 80-81 2.1819404158204895E-4 82-83 7.23157394957648E-4 84-85 3.272910623730734E-4 86-87 3.8963221711080167E-4 88-89 9.912243603298794E-4 90-91 1.5273582910743425E-4 92-93 1.6832111779186633E-4 94-95 0.0030921212749913224 96-97 4.114516212690066E-4 98-99 0.0015616459261800929 100 0.001365271288756249 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.6040768E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.92030809375963 #Duplication Level Percentage of deduplicated Percentage of total 1 95.24813303038607 83.74245201386947 2 4.269852965279576 7.508135764448667 3 0.32051873725297114 0.8454031838731205 4 0.051952524591003094 0.1827072787315846 5 0.027440000596134426 0.12062666532525433 6 0.015355272440447867 0.08100241702966757 7 0.011496386923892056 0.07075361162195612 8 0.007774076190147581 0.054679933902570914 9 0.00498700602830509 0.039461319582661795 >10 0.035097024326405026 0.5872034408003556 >50 0.0035814534105278024 0.21996262446273696 >100 0.002489893868316706 0.43803881103430065 >500 2.868999703033743E-4 0.18259390376295206 >1k 6.095317818790913E-4 1.274322421345628 >5k 1.8425201340841287E-4 1.1219894007510243 >10k+ 2.409449406110014E-4 3.53066720945802 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29794 0.1857392364255876 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24044 0.14989307245139386 No Hit TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23408 0.14592817501007432 No Hit TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22901 0.1427674784648715 No Hit TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21434 0.13362203106484677 No Hit CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21313 0.13286770309252027 No Hit TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19627 0.12235698440373928 No Hit CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19177 0.11955163244054151 No Hit TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18922 0.11796193299472943 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18612 0.11602935719785985 No Hit TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18472 0.11515658103153166 No Hit ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18228 0.1136354568559311 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17434 0.10868556916975546 No Hit TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17293 0.1078065588879535 No Hit CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17139 0.10684650510499247 No Hit CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16620 0.10361099917410438 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012842277875972023 0.0 0.0 0.0 0.0 2 0.003210569468993006 0.0 0.0 1.2468230947545654E-5 0.0 3 0.005654342734711954 0.0 0.0 3.117057736886413E-5 0.0 4 0.00992471183424634 0.0 0.0 3.7404692842636964E-5 0.0 5 0.01733084101708846 0.0 0.0 3.7404692842636964E-5 0.0 6 0.03104589505938868 0.0 0.0 3.7404692842636964E-5 0.0 7 0.04830816080626563 0.0 0.0 3.7404692842636964E-5 0.0 8 0.12258764667626887 0.0 0.0 3.7404692842636964E-5 0.0 9 0.40610275019250947 0.0 0.0 4.363880831640979E-5 0.0 10-11 0.9577752137553515 0.0 0.0 4.363880831640979E-5 0.0 12-13 1.5441592322761601 0.0 0.0 4.363880831640979E-5 0.0 14-15 2.168502779916772 0.0 0.0 6.234115473772826E-5 0.0 16-17 2.734728786053137 0.0 0.0 7.480938568527393E-5 0.0 18-19 3.3187874795022285 0.0 0.0 7.480938568527393E-5 0.0 20-21 4.052196877356495 0.0 6.234115473772827E-6 7.480938568527393E-5 0.0 22-23 4.945713945865934 0.0 6.234115473772827E-6 8.104350115904675E-5 0.0 24-25 6.054027463024214 0.0 6.234115473772827E-6 8.727761663281956E-5 0.0 26-27 7.396282397451294 0.0 6.234115473772827E-6 9.35117321065924E-5 0.0 28-29 9.122253373404565 0.0 6.234115473772827E-6 9.974584758036523E-5 0.0 30-31 11.184233822220982 0.0 6.234115473772827E-6 9.974584758036523E-5 0.0 32-33 13.39852929735035 0.0 6.234115473772827E-6 1.0909702079102448E-4 0.0 34-35 15.298909004855627 0.0 6.234115473772827E-6 1.1844819400168371E-4 0.0 36-37 16.636890453125435 0.0 6.234115473772827E-6 1.2156525173857011E-4 0.0 38-39 17.55915302808444 0.0 6.234115473772827E-6 1.5273582910743427E-4 0.0 40-41 18.36398045280625 0.0 6.234115473772827E-6 1.7767229100252557E-4 0.0 42-43 19.01724717918743 0.0 6.234115473772827E-6 2.306622725295946E-4 0.0 44-45 19.555001979955076 0.0 6.234115473772827E-6 2.587157921615723E-4 0.0 46-47 20.012813600944792 0.0 6.234115473772827E-6 2.7118402310911797E-4 0.0 48-49 20.421397529095863 0.0 6.234115473772827E-6 2.961204850042093E-4 0.0 50-51 20.81476086431772 0.0 6.234115473772827E-6 3.5222752426816473E-4 0.0 52-53 21.22152443075045 0.0 6.234115473772827E-6 3.802810439001424E-4 0.0 54-55 21.62262742033299 0.0 6.234115473772827E-6 4.0833456353212016E-4 0.0 56-57 22.030750023939003 0.0 6.234115473772827E-6 4.363880831640979E-4 0.0 58-59 22.42614568080531 0.0 6.234115473772827E-6 4.5509042958541635E-4 0.0 60-61 22.810416558608665 0.0 6.234115473772827E-6 4.613245450591892E-4 0.0 62-63 23.189298043584948 0.0 6.234115473772827E-6 4.893780646911669E-4 0.0 64-65 23.564934671457127 0.0 6.234115473772827E-6 5.454851039551224E-4 0.0 66-67 23.924789636007453 0.0 6.234115473772827E-6 6.265286051141691E-4 0.0 68-69 24.26286883520789 0.0 6.234115473772827E-6 6.85752702115011E-4 0.0 70-71 24.59044666689276 0.0 6.234115473772827E-6 7.106891640101023E-4 0.0 72-73 24.917507066993302 0.0 6.234115473772827E-6 7.356256259051936E-4 0.0 74-75 25.239589525888036 0.0 6.234115473772827E-6 7.761473764847169E-4 0.0 76-77 25.558847930473156 3.1170577368864134E-6 9.351173210659241E-6 8.353714734855588E-4 0.0 78-79 25.876750414942727 6.234115473772827E-6 1.2468230947545654E-5 9.382343788028104E-4 0.0 80-81 26.191202316497566 6.234115473772827E-6 1.2468230947545654E-5 0.0010660337460151534 0.0 82-83 26.505610579244085 6.234115473772827E-6 1.2468230947545654E-5 0.0011096725543315632 0.0 84-85 26.825330308374262 6.234115473772827E-6 1.2468230947545654E-5 0.0011501943049110866 0.0 86-87 27.150115256326877 6.234115473772827E-6 1.5585288684432066E-5 0.0011657795935955187 0.0 88 27.39643762692659 6.234115473772827E-6 1.8702346421318482E-5 0.0011657795935955187 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCCTA 6970 0.0 54.416286 6 GCCCTAC 17160 0.0 49.600872 7 TGCTCTA 7265 0.0 47.225372 6 CCCGGAA 3265 0.0 45.919342 6 CCCCTAC 45140 0.0 44.42723 7 TTGCTCT 2635 0.0 43.699486 5 GCCTTTT 2175 0.0 42.81448 1 TTGCCCT 3200 0.0 42.152447 5 CCCCCTA 25105 0.0 41.803886 6 GCCCCTA 5155 0.0 41.756584 6 CCCCGGA 1155 0.0 41.505825 5 GTCCCTA 2185 0.0 41.298847 6 CCGGAAG 10835 0.0 40.774353 7 CTGCCCT 3835 0.0 40.565186 5 TGCTCGG 570 0.0 40.40286 5 ATTGCTC 705 0.0 39.999355 4 CTCCGGA 1260 0.0 39.912052 5 CGCCCTA 7180 0.0 39.14397 6 CTGCGTC 1705 0.0 39.142822 6 TTGCGTC 1840 0.0 38.82522 6 >>END_MODULE