##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248489_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18620980
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.32595765636395	33.0	31.0	34.0	25.0	34.0
2	30.63654721717117	33.0	31.0	34.0	26.0	34.0
3	30.83510615445589	34.0	31.0	34.0	26.0	34.0
4	34.254175881183485	37.0	35.0	37.0	30.0	37.0
5	34.18788039082798	37.0	35.0	37.0	30.0	37.0
6	34.093957246074055	37.0	35.0	37.0	29.0	37.0
7	34.14612415673074	37.0	35.0	37.0	30.0	37.0
8	34.09308355414162	37.0	35.0	37.0	29.0	37.0
9	35.58525469658417	39.0	35.0	39.0	28.0	39.0
10-11	35.851694432838656	39.0	35.0	39.0	30.0	39.0
12-13	35.87019925911525	39.0	35.0	39.0	30.0	39.0
14-15	37.017450639010406	40.0	37.0	41.0	30.0	41.0
16-17	36.867357464537314	40.0	37.0	41.0	30.0	41.0
18-19	36.49304475382068	39.0	35.5	41.0	30.0	41.0
20-21	36.21148763384097	39.0	35.0	41.0	28.0	41.0
22-23	36.00524459507502	39.0	35.0	41.0	27.5	41.0
24-25	35.82298302774612	39.0	35.0	41.0	26.5	41.0
26-27	35.65458799697975	39.0	35.0	41.0	25.0	41.0
28-29	35.49602679343407	39.0	35.0	40.5	25.0	41.0
30-31	35.43061326525242	38.5	35.0	40.0	25.0	41.0
32-33	35.33827110603201	38.0	34.5	40.0	25.0	41.0
34-35	35.21040774975323	38.0	34.0	40.0	25.0	41.0
36-37	35.09851949252939	38.0	34.0	40.0	25.0	41.0
38-39	34.92761366480175	38.0	33.0	40.0	25.0	41.0
40-41	34.74343101168682	38.0	33.0	40.0	25.0	41.0
42-43	34.501027523793056	38.0	33.0	40.0	25.0	41.0
44-45	34.3102888784586	37.0	33.0	40.0	24.5	41.0
46-47	34.159870774792736	37.0	33.0	40.0	24.5	41.0
48-49	34.081124516539944	36.5	33.0	39.0	25.0	41.0
50-51	33.817623401131414	36.0	33.0	39.0	25.0	41.0
52-53	33.451327266341515	35.0	32.0	39.0	24.0	40.0
54-55	33.270788997141935	35.0	32.0	38.0	25.0	40.0
56-57	33.134019450104134	35.0	32.0	37.0	25.0	40.0
58-59	32.85127882635608	35.0	32.0	37.0	25.0	39.0
60-61	32.57528142987104	35.0	32.0	36.0	25.0	39.0
62-63	32.293140720842835	35.0	31.0	35.5	24.0	38.5
64-65	32.08791913744604	35.0	31.0	35.0	24.0	37.0
66-67	31.909021034338686	35.0	31.0	35.0	24.0	37.0
68-69	31.742236203465126	35.0	31.0	35.0	23.0	36.5
70-71	31.60788172802935	35.0	31.0	35.0	23.0	36.0
72-73	31.502458812586664	35.0	31.0	35.0	23.0	36.0
74-75	31.393035114156184	35.0	30.5	35.0	23.0	35.5
76-77	31.2543959286783	35.0	30.0	35.0	23.0	35.0
78-79	31.12535011583708	35.0	30.0	35.0	21.5	35.0
80-81	30.96403194139084	35.0	30.0	35.0	20.0	35.0
82-83	30.812395641904992	35.0	30.0	35.0	20.0	35.0
84-85	30.63522999326566	35.0	30.0	35.0	19.5	35.0
86-87	30.38152097795068	34.0	29.0	35.0	17.5	35.0
88-89	30.136645815633763	34.0	29.0	35.0	16.0	35.0
90-91	29.82399454808501	34.0	28.5	35.0	12.5	35.0
92-93	29.480257000437142	34.0	28.0	35.0	10.0	35.0
94-95	29.10413635587386	34.0	27.0	35.0	10.0	35.0
96-97	28.725664975742415	34.0	26.0	35.0	8.0	35.0
98-99	28.280533811861673	33.5	25.0	35.0	8.0	35.0
100	26.96448296491377	32.0	23.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5466247081989799
1101	2	0.5197801742985249
1101	3	0.4682081466871537
1101	4	0.41313191658719717
1101	5	0.4086869819760892
1101	6	0.3888630347096864
1101	7	0.3988058484718948
1101	8	0.3836774825953242
1101	9	0.4677999266004207
1101	10-11	0.46295933073152185
1101	12-13	0.4856924183922544
1101	14-15	0.5914829993372877
1101	16-17	0.6197136961018259
1101	18-19	0.6064310877427133
1101	20-21	0.6462447054471525
1101	22-23	0.6405245148373311
1101	24-25	0.6739463646354196
1101	26-27	0.6899965755691255
1101	28-29	0.6804279271762752
1101	30-31	0.6363249487214091
1101	32-33	0.6498743295895366
1101	34-35	0.6664947768699321
1101	36-37	0.6417566032266251
1101	38-39	0.6304215819300225
1101	40-41	0.6456808632376791
1101	42-43	0.6260272595173504
1101	44-45	0.6340823137564513
1101	46-47	0.708821122188283
1101	48-49	0.6761952483177751
1101	50-51	0.6459961901126547
1101	52-53	0.6112354365119259
1101	54-55	0.5491946381694035
1101	56-57	0.5555280584876243
1101	58-59	0.5919268309892232
1101	60-61	0.5197158488979525
1101	62-63	0.5736993908829717
1101	64-65	0.5182775972635483
1101	66-67	0.4938008448098117
1101	68-69	0.5425863143348799
1101	70-71	0.558860738291223
1101	72-73	0.5355038996982984
1101	74-75	0.5321514989858898
1101	76-77	0.5394105552457908
1101	78-79	0.5636004893128828
1101	80-81	0.5130012797661543
1101	82-83	0.5273922854843995
1101	84-85	0.4668153584895194
1101	86-87	0.4618833634813768
1101	88-89	0.4451990250082112
1101	90-91	0.41205923741665984
1101	92-93	0.38263447227615544
1101	94-95	0.389413258313585
1101	96-97	0.39182163454487196
1101	98-99	0.3783669924333317
1101	100	0.37957973438960124
1102	1	0.3718480263286459
1102	2	0.3976788957288271
1102	3	0.34916431110877255
1102	4	0.3094729678933561
1102	5	0.3087906837200407
1102	6	0.28334077658615797
1102	7	0.25592034903691285
1102	8	0.2642365035268597
1102	9	0.27159237432510963
1102	10-11	0.38165833707887487
1102	12-13	0.44346078110681475
1102	14-15	0.5717263759188427
1102	16-17	0.5767621910544065
1102	18-19	0.5741539407756591
1102	20-21	0.5665794256948686
1102	22-23	0.5560659838600728
1102	24-25	0.5823692884927638
1102	26-27	0.6028999090788858
1102	28-29	0.587905044640344
1102	30-31	0.6022445480740757
1102	32-33	0.61647505693103
1102	34-35	0.6450668885411943
1102	36-37	0.6462389062337905
1102	38-39	0.6368889721199409
1102	40-41	0.6613676015615226
1102	42-43	0.6488765656307365
1102	44-45	0.6274399396533283
1102	46-47	0.6268073936006431
1102	48-49	0.5846854381528033
1102	50-51	0.5730763174942837
1102	52-53	0.5591516589362797
1102	54-55	0.558430056002841
1102	56-57	0.5124261201969205
1102	58-59	0.5109104674131544
1102	60-61	0.506076510051706
1102	62-63	0.5245198374593159
1102	64-65	0.48615510233560144
1102	66-67	0.47415213835878944
1102	68-69	0.49731066871137486
1102	70-71	0.4998983476550407
1102	72-73	0.47278536798812354
1102	74-75	0.4942881849223255
1102	76-77	0.4769929078840285
1102	78-79	0.4808183427886803
1102	80-81	0.46305914412331717
1102	82-83	0.5051148907311322
1102	84-85	0.5087659474220203
1102	86-87	0.5237327186314644
1102	88-89	0.5093634911504061
1102	90-91	0.47190355626354474
1102	92-93	0.4092634001243489
1102	94-95	0.39447580990600883
1102	96-97	0.45505032243952215
1102	98-99	0.45184696208236375
1102	100	0.3896912523714384
1103	1	0.3911733377053501
1103	2	0.3483383951794181
1103	3	0.3194072738324145
1103	4	0.30111517666267673
1103	5	0.28984341532765256
1103	6	0.2755153095152849
1103	7	0.2926908402478574
1103	8	0.257865728945589
1103	9	0.28666526611794296
1103	10-11	0.4108922769220982
1103	12-13	0.4313283174781972
1103	14-15	0.5403227954532639
1103	16-17	0.5407379061294648
1103	18-19	0.5763808917695457
1103	20-21	0.5541217175358
1103	22-23	0.5785711931886368
1103	24-25	0.5813088211846278
1103	26-27	0.5835222066412911
1103	28-29	0.5883482608419754
1103	30-31	0.5826275867914461
1103	32-33	0.5668246454471202
1103	34-35	0.5934175791857612
1103	36-37	0.6089766482041554
1103	38-39	0.6188503640077627
1103	40-41	0.6000431482317552
1103	42-43	0.624635156059874
1103	44-45	0.6247222274412323
1103	46-47	0.6196290139396652
1103	48-49	0.5802265299009335
1103	50-51	0.540229121441854
1103	52-53	0.5241960664712764
1103	54-55	0.5094207828639625
1103	56-57	0.5032557667350517
1103	58-59	0.4679819630138411
1103	60-61	0.44692729295951494
1103	62-63	0.4550514267175956
1103	64-65	0.43838764032987143
1103	66-67	0.47322219461266
1103	68-69	0.4692175733644923
1103	70-71	0.43460599768531694
1103	72-73	0.462223153951939
1103	74-75	0.45833668808212735
1103	76-77	0.43567650505845634
1103	78-79	0.46982169887443703
1103	80-81	0.44862577640091317
1103	82-83	0.4209787654864954
1103	84-85	0.3443100981279379
1103	86-87	0.361268734446476
1103	88-89	0.36299619506119285
1103	90-91	0.4006185970757983
1103	92-93	0.3502227741978956
1103	94-95	0.37677421402382905
1103	96-97	0.33430548769919
1103	98-99	0.39411146308416534
1103	100	0.29012477696751304
1104	1	0.22712272829907576
1104	2	0.20995173357044905
1104	3	0.21268188330759585
1104	4	0.2111459323977698
1104	5	0.14506219632445294
1104	6	0.14267172257573435
1104	7	0.1538247139613631
1104	8	0.16252093555060299
1104	9	0.24521823254316644
1104	10-11	0.29458860420371025
1104	12-13	0.32744697221780683
1104	14-15	0.3777228551216609
1104	16-17	0.4066909744268514
1104	18-19	0.39605388674089426
1104	20-21	0.4078236695370734
1104	22-23	0.3834062320810361
1104	24-25	0.3834701771966351
1104	26-27	0.3965324524110514
1104	28-29	0.3738005068442689
1104	30-31	0.3420634335923296
1104	32-33	0.36650745329178847
1104	34-35	0.44825781587545777
1104	36-37	0.39924505245629405
1104	38-39	0.4111722675886824
1104	40-41	0.3957029909782648
1104	42-43	0.4177231715449281
1104	44-45	0.3858628684704968
1104	46-47	0.41134218678124057
1104	48-49	0.3840156142972404
1104	50-51	0.3650119700075294
1104	52-53	0.34207738391541653
1104	54-55	0.34898073870711954
1104	56-57	0.3311464962230417
1104	58-59	0.3275966677142179
1104	60-61	0.28011334196902027
1104	62-63	0.32734677558148206
1104	64-65	0.3077166448243034
1104	66-67	0.28583340556131986
1104	68-69	0.33173130350314395
1104	70-71	0.32724729447929946
1104	72-73	0.3162897762693717
1104	74-75	0.32623814454458966
1104	76-77	0.2822785121664211
1104	78-79	0.32468617162332336
1104	80-81	0.3308059239755181
1104	82-83	0.3015838020775874
1104	84-85	0.29963276999011157
1104	86-87	0.33651353115980953
1104	88-89	0.30531954421927665
1104	90-91	0.25710284821723306
1104	92-93	0.24436351331523554
1104	94-95	0.2695566582526716
1104	96-97	0.25215676735750137
1104	98-99	0.2449833264900505
1104	100	0.21861054236506305
1105	1	0.20138123087111026
1105	2	0.20018382724002137
1105	3	0.20272249722278346
1105	4	0.1606698976247145
1105	5	0.15162124338255722
1105	6	0.10245608493046632
1105	7	0.1351789588480088
1105	8	0.11008514625414278
1105	9	0.1828996271930734
1105	10-11	0.2603741247664999
1105	12-13	0.2663541830522931
1105	14-15	0.32232216489382637
1105	16-17	0.343574840604731
1105	18-19	0.317334886758168
1105	20-21	0.3029164548579715
1105	22-23	0.3294799976887788
1105	24-25	0.35243572384519695
1105	26-27	0.36305627679438146
1105	28-29	0.32366835807264493
1105	30-31	0.3231352401577894
1105	32-33	0.34229534425071506
1105	34-35	0.3613566866203044
1105	36-37	0.3191004327667386
1105	38-39	0.3666948723105179
1105	40-41	0.3635312282025893
1105	42-43	0.3563064007587471
1105	44-45	0.3797858236867029
1105	46-47	0.357853932219129
1105	48-49	0.3311314472953697
1105	50-51	0.3195827637452595
1105	52-53	0.2826288468166851
1105	54-55	0.27664418233509736
1105	56-57	0.26789646772439824
1105	58-59	0.22878476406488346
1105	60-61	0.21206008358714
1105	62-63	0.25247691607324185
1105	64-65	0.21573970423106914
1105	66-67	0.1743850733783603
1105	68-69	0.2171606274348541
1105	70-71	0.21541787041948623
1105	72-73	0.19772904920009182
1105	74-75	0.26670850070939167
1105	76-77	0.21829723598509432
1105	78-79	0.22928338616570443
1105	80-81	0.24345095368797232
1105	82-83	0.20278407328767045
1105	84-85	0.2100959926642787
1105	86-87	0.2343710117302713
1105	88-89	0.23547612610568436
1105	90-91	0.19694999445795602
1105	92-93	0.1786633992150115
1105	94-95	0.18306657636668078
1105	96-97	0.18944382404466964
1105	98-99	0.1700971144110781
1105	100	0.13777749167772768
1106	1	0.1219660782693488
1106	2	0.11739845331660703
1106	3	0.12486070860759924
1106	4	0.12596386317875385
1106	5	0.12775497171834616
1106	6	0.0664029379644191
1106	7	0.05301155448957928
1106	8	0.13420296495504402
1106	9	0.10565158879547454
1106	10-11	0.18801314859776141
1106	12-13	0.23013575187608382
1106	14-15	0.269143433736609
1106	16-17	0.26012883536236586
1106	18-19	0.28287651793549884
1106	20-21	0.26606944229945384
1106	22-23	0.2569968701024692
1106	24-25	0.2829061490937548
1106	26-27	0.28853517751736035
1106	28-29	0.21441765310817829
1106	30-31	0.2619945576988556
1106	32-33	0.2612993860420687
1106	34-35	0.30152717774515025
1106	36-37	0.26942582686575633
1106	38-39	0.26700908169061677
1106	40-41	0.2556652657438576
1106	42-43	0.2671398404504899
1106	44-45	0.27450201287011566
1106	46-47	0.2788424072999902
1106	48-49	0.27704379919045863
1106	50-51	0.26753771013028427
1106	52-53	0.28561959032298034
1106	54-55	0.19899575421434434
1106	56-57	0.19437425721968538
1106	58-59	0.1773718815189227
1106	60-61	0.1593026926818908
1106	62-63	0.16509918940568014
1106	64-65	0.18929105402615676
1106	66-67	0.14670000541859451
1106	68-69	0.18073641031348942
1106	70-71	0.1569367340754937
1106	72-73	0.17153955506421426
1106	74-75	0.19031514292110074
1106	76-77	0.17255262045962283
1106	78-79	0.17801358694527636
1106	80-81	0.19687358533196786
1106	82-83	0.1892069906045215
1106	84-85	0.18297057996427313
1106	86-87	0.23206069282020536
1106	88-89	0.17547654332314622
1106	90-91	0.20453163138524388
1106	92-93	0.19434404313040332
1106	94-95	0.174295424165404
1106	96-97	0.18112136474936236
1106	98-99	0.24887009654566583
1106	100	0.1694601230202295
1107	1	0.11781064127865903
1107	2	0.10831671491743577
1107	3	0.14667213557964232
1107	4	0.14978039936018916
1107	5	0.14443345133922492
1107	6	0.12321591439526003
1107	7	0.05601243812026979
1107	8	0.09022156075931775
1107	9	0.09918101249078148
1107	10-11	0.15058207199869855
1107	12-13	0.18748580370058932
1107	14-15	0.19852145984489766
1107	16-17	0.20014206067077112
1107	18-19	0.18135554694331546
1107	20-21	0.17693419826326107
1107	22-23	0.16802003719632097
1107	24-25	0.17608299553712214
1107	26-27	0.19650535785841328
1107	28-29	0.2044123272255689
1107	30-31	0.15048946868434854
1107	32-33	0.149715177594949
1107	34-35	0.19228065468391975
1107	36-37	0.16110237515864867
1107	38-39	0.1886905028695196
1107	40-41	0.1670224523139865
1107	42-43	0.1482562989968912
1107	44-45	0.20923607458280458
1107	46-47	0.265881110349369
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2216	78-79	-0.6019004712868252
2216	80-81	-0.6573545156706224
2216	82-83	-0.6193653964447456
2216	84-85	-0.6799250020257723
2216	86-87	-0.6279997671206701
2216	88-89	-0.6201764134135779
2216	90-91	-0.5515457515759756
2216	92-93	-0.6175656007150998
2216	94-95	-0.5946802716118036
2216	96-97	-0.6233659905835331
2216	98-99	-0.6704605216901172
2216	100	-0.6803146066415309
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	8.0
3	15.0
4	28.0
5	781.0
6	5438.0
7	15072.0
8	33448.0
9	53373.0
10	70417.0
11	84247.0
12	90185.0
13	93580.0
14	97227.0
15	102011.0
16	105372.0
17	108848.0
18	114695.0
19	120614.0
20	128491.0
21	136472.0
22	145773.0
23	167184.0
24	251933.0
25	313574.0
26	247491.0
27	443517.0
28	312942.0
29	483275.0
30	467504.0
31	594289.0
32	763963.0
33	1219153.0
34	1364719.0
35	2847465.0
36	3297809.0
37	3222376.0
38	915521.0
39	200786.0
40	1383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.055003417655552	28.04576801443879	26.130785364786934	28.76844320311872
2	14.891164159303253	29.169994470725563	28.563127601648215	27.375713768322974
3	13.580053715778101	31.464871973385705	25.474357622147757	29.480716688688428
4	12.616618633579732	29.856099441244478	26.00304658569469	31.524235339481105
5	12.587458253861634	30.25170018781063	26.568969550532618	30.591872007795118
6	12.399035767852846	27.141125364625317	27.460885598690112	32.99895326883172
7	17.602978856428418	30.633037601908015	23.008648859339676	28.75533468232389
8	14.944497184245956	29.112801851824898	20.706359732657628	35.23634123127152
9	11.719585796538158	27.384105072918885	22.54077770796971	38.35553142257325
10-11	18.78542839949445	38.9986702858578	21.19058084108038	21.025320473567376
12-13	18.27279109178174	40.66794183373586	19.660962444849392	21.39830462963301
14-15	20.022107877592195	39.82734470459244	18.794585240191033	21.35596217762433
16-17	19.921266838781168	39.375586694484035	19.466875745321342	21.23627072141346
18-19	20.8566584399888	37.09084674453681	20.852332519616343	21.200162295858046
20-21	19.20009617479366	36.77035504894977	22.392038114820448	21.637510661436128
22-23	19.433738973448413	37.96068985421381	23.19847518958091	19.407095982756868
24-25	19.48223697758816	38.121656375292574	22.229174706278208	20.166931940841064
26-27	21.677847764103646	38.64815749409939	20.40902821634477	19.2649665254522
28-29	20.79369895608568	39.80565116101521	21.54683279768108	17.853817085218026
30-31	21.068705824146083	39.21671578354658	21.50922169397997	18.205356698327364
32-33	21.792603017086588	39.51389350813662	20.658706859541926	18.034796615234868
34-35	21.930158004491087	39.52692723905713	20.452248725412677	18.090666031039103
36-37	22.60069910954529	39.20191237211531	20.06357440654412	18.133814111795278
38-39	23.12483150841681	39.393676550995984	19.904108937557567	17.577383003029635
40-41	23.541476577312654	39.61203001170867	19.783570833919413	17.062922577059265
42-43	23.84779852856705	39.85418805870426	20.003283002818158	16.29473040991053
44-45	24.128374092077046	39.53030111967945	19.963050964845706	16.3782738233978
46-47	24.318659328039384	38.95403588651149	20.336438159905885	16.390866625543243
48-49	24.170357292898974	39.04325810027843	20.840220823980054	15.946163782842545
50-51	24.341818736249333	38.8995432199648	21.21141995468746	15.547218089098408
52-53	24.41913648828724	38.999515982317575	21.41948355783206	15.161863971563122
54-55	24.39421586190111	39.21812933548813	21.442125858827023	14.94552894378374
56-57	24.277085745934244	39.32173405165903	21.008800496785994	15.392379705620732
58-59	24.08662974326629	39.14890226606074	21.136518118045174	15.627949872627802
60-61	24.01608517044527	38.72124618958162	21.529851711192943	15.732816928780164
62-63	24.186144322424312	37.98111856439261	21.808896506576183	16.023840606606896
64-65	24.06405814861036	37.709884477469636	21.861746774218147	16.364310599701852
66-67	23.66926052296513	37.38824538234697	22.25412050633825	16.688373588349652
68-69	23.46711004680971	37.37333626370699	21.984204555549432	17.175349133933867
70-71	23.020366259130228	37.420003507210595	22.17691433678406	17.382715896875116
72-73	22.968588067499834	37.84501143533571	22.195086561340855	16.9913139358236
74-75	22.6799468159504	38.45673387113234	22.024895029502172	16.83842428341508
76-77	21.999386810287128	39.36269199307766	22.0516577956657	16.58626340096951
78-79	21.406161163533625	40.39179961783114	21.73489352045194	16.467145698183288
80-81	20.758484207952495	41.771931495394675	21.26103523534915	16.208549061303675
82-83	20.17595736531107	42.997417461385865	21.092979071279817	15.733646102023247
84-85	19.588018626600558	44.30117240498767	20.910211193181123	15.200597775230648
86-87	19.112701435575733	45.42217552422647	20.5568821936673	14.908240846530504
88-89	18.790900333471164	46.27447950255096	20.335050912337614	14.599569251640258
90-91	18.7268414210845	47.01869864142066	19.928540124652596	14.325919812842237
92-93	18.810491708318313	47.47631065110641	19.48003618860293	14.233161451972348
94-95	19.046246674210153	47.6342806953201	19.067568146799587	14.251904483670153
96-97	19.318544421644724	47.42463498371792	18.889086170347998	14.367734424289358
98-99	19.69118152315403	46.988239268239276	18.73167870251583	14.588900506090866
100	20.411861026043045	46.574178143134034	18.32401814534701	14.689942685475907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1115022.0
1	994530.5
2	564106.0
3	163829.5
4	70239.0
5	62811.0
6	50877.0
7	36009.5
8	25635.0
9	21635.5
10	22029.5
11	25301.0
12	31467.0
13	40783.0
14	53168.0
15	69177.5
16	86721.5
17	104858.5
18	123376.5
19	141166.0
20	156869.5
21	169123.5
22	179721.5
23	188637.0
24	193853.0
25	196188.0
26	196240.0
27	193328.5
28	186865.0
29	177462.5
30	166356.0
31	154577.5
32	142900.5
33	131985.5
34	122798.0
35	114939.0
36	108787.5
37	105544.5
38	106761.0
39	113986.5
40	128479.0
41	153456.5
42	191806.5
43	246040.0
44	316221.0
45	397513.5
46	481468.5
47	558972.0
48	627602.5
49	695798.5
50	776105.5
51	874770.5
52	978351.0
53	1055512.5
54	1066696.5
55	988940.0
56	831469.5
57	628732.0
58	429535.5
59	268713.0
60	157184.0
61	90206.0
62	54354.0
63	35890.0
64	26468.5
65	21381.0
66	17935.0
67	15396.5
68	13723.0
69	12161.5
70	10620.0
71	9331.0
72	8280.5
73	7419.0
74	6726.5
75	6116.0
76	5532.0
77	5044.5
78	4813.5
79	4610.0
80	4438.0
81	4334.5
82	4173.5
83	3933.0
84	3756.5
85	3679.5
86	3479.5
87	3349.5
88	3218.5
89	3039.0
90	2909.5
91	2772.0
92	2587.0
93	2352.5
94	2191.5
95	2018.0
96	1802.5
97	1591.0
98	1341.5
99	1112.5
100	937.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.50368025743006E-4
2	4.940663702984483E-4
3	4.296229306943029E-5
4	7.249886955466361E-4
5	5.155475168331634E-4
6	8.216538549528543E-4
7	7.679509886160665E-4
8	0.0011438710529735815
9	4.4573379059533927E-4
10-11	3.249023413375665E-4
12-13	8.162835683191756E-4
14-15	0.0025132941445616718
16-17	0.0012700727888650329
18-19	0.003571240611396393
20-21	0.0038478103730308503
22-23	0.0037484600703077925
24-25	0.0050292734324401825
26-27	0.007040445776752888
28-29	0.0095564250646314
30-31	0.00959670221438399
32-33	0.009271799873046424
34-35	0.011333989940379078
36-37	0.010791590990377522
38-39	0.009271799873046424
40-41	0.007770804758933204
42-43	0.00446539333590391
44-45	0.005413248926748216
46-47	0.007633862449774394
48-49	0.0062966610779883764
50-51	0.004019659545308571
52-53	0.004255952157190437
54-55	0.0043821538930818894
56-57	0.006941095474029831
58-59	0.0082272791227959
60-61	0.007891636208190975
62-63	0.00933892845596741
64-65	0.0103539126297327
66-67	0.009274485016363262
68-69	0.011782408874291257
70-71	0.011787779160924935
72-73	0.013927838384445931
74-75	0.018087125382230153
76-77	0.019209515288669016
78-79	0.019091368982728084
80-81	0.023430560582740543
82-83	0.02162614427382447
84-85	0.021207261916397525
86-87	0.021236798492882757
88-89	0.02190002889214209
90-91	0.021260964782734315
92-93	0.021851696312438978
94-95	0.01932229130797627
96-97	0.02167716199684442
98-99	0.01865369062208326
100	0.018983963250054508
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.862098E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.18310661253037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.16892210685754	81.79981729146247
2	2.5684264228318456	4.324362307593866
3	0.15073323586407228	0.38067576194390645
4	0.03499202831002154	0.11782950599244899
5	0.016889646290246925	0.07109114471498926
6	0.010411734416954407	0.052589528906629476
7	0.007889473404281753	0.04649122664965615
8	0.005326375177342454	0.03587126475300441
9	0.003086812632317713	0.023387172922737676
>10	0.026335822579713723	0.44186256894650455
>50	0.0032410542324418966	0.1945999073071044
>100	0.0026894467170978324	0.43761085214875384
>500	3.939139775224268E-4	0.2494493184045056
>1k	3.9464901622164686E-4	0.7513917228895021
>5k	7.636505497548841E-5	0.44268202849593363
>10k+	1.909126374387202E-4	10.63028839686799
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	805158	4.323929245399544	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	176046	0.9454174807126156	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	155680	0.8360462231311134	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	132824	0.7133029518317511	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72848	0.39121464069023215	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64285	0.34522887624604076	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53028	0.28477555961071865	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46002	0.2470439257224915	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45491	0.24429970925268166	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44222	0.23748481551454326	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35900	0.19279329014906843	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29832	0.16020639085590554	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28675	0.15399296922073918	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23627	0.12688376229392867	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23257	0.12489675623946753	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23166	0.12440806015580276	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22610	0.12142218078747735	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21144	0.11354934058250424	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21086	0.11323786395775089	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20149	0.10820590538199386	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19687	0.10572483295723425	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0032006908336725563	0.0	0.0	0.0	5.370286633678786E-6
2	0.00729821953516947	0.0	0.0	5.370286633678786E-6	5.370286633678786E-6
3	0.011830741453994365	0.0	0.0	4.296229306943029E-5	5.370286633678786E-6
4	0.018919519810450362	0.0	0.0	4.296229306943029E-5	5.370286633678786E-6
5	0.030986553876326595	0.0	0.0	5.370286633678786E-5	5.370286633678786E-6
6	0.05292417477490444	0.0	0.0	5.370286633678786E-5	5.370286633678786E-6
7	0.07859414488388904	0.0	0.0	5.370286633678786E-5	5.370286633678786E-6
8	0.1706999309381139	0.0	0.0	5.370286633678786E-5	5.370286633678786E-6
9	0.5204774399628805	0.0	0.0	5.370286633678786E-5	5.370286633678786E-6
10-11	1.298658824616105	0.0	0.0	5.638800965362725E-5	5.370286633678786E-6
12-13	2.358176100291177	0.0	0.0	5.9073152970466645E-5	5.370286633678786E-6
14-15	3.563370456334736	0.0	0.0	5.9073152970466645E-5	5.370286633678786E-6
16-17	4.86926037190309	0.0	0.0	6.444343960414544E-5	5.370286633678786E-6
18-19	6.286911322604933	0.0	0.0	6.444343960414544E-5	5.370286633678786E-6
20-21	8.116119559765384	0.0	0.0	6.444343960414544E-5	5.370286633678786E-6
22-23	10.171406123630444	0.0	0.0	6.444343960414544E-5	5.370286633678786E-6
24-25	12.326512890299007	0.0	0.0	6.712858292098482E-5	5.370286633678786E-6
26-27	14.519133794247136	0.0	0.0	7.518401287150301E-5	5.370286633678786E-6
28-29	16.839247988022112	0.0	0.0	8.323944282202119E-5	5.370286633678786E-6
30-31	19.295630519983376	0.0	0.0	9.398001608937876E-5	5.370286633678786E-6
32-33	22.028416334693446	0.0	0.0	9.666515940621815E-5	5.370286633678786E-6
34-35	24.560836755100965	0.0	0.0	9.666515940621815E-5	1.0740573267357572E-5
36-37	26.67451981582065	0.0	0.0	1.0203544603989693E-4	1.8796003217875752E-5
38-39	28.347286233055403	0.0	0.0	1.1814630594093329E-4	2.1481146534715144E-5
40-41	29.853600616079284	0.0	0.0	1.3962745247564844E-4	2.1481146534715144E-5
42-43	31.13752068902926	0.0	0.0	1.4499773910932722E-4	2.685143316839393E-5
44-45	32.231542056325715	0.0	0.0	1.530531690598454E-4	2.685143316839393E-5
46-47	33.20037935704781	0.0	0.0	1.6110859901036359E-4	2.685143316839393E-5
48-49	34.114259829504135	0.0	0.0	1.960154621292757E-4	2.685143316839393E-5
50-51	35.02927343244018	0.0	0.0	2.362926118818666E-4	2.685143316839393E-5
52-53	35.98434668852015	0.0	0.0	3.034211948028514E-4	2.685143316839393E-5
54-55	36.97100797057942	0.0	0.0	3.6786463440699685E-4	2.685143316839393E-5
56-57	37.996912622214296	0.0	0.0	3.89345780941712E-4	3.7592006435751504E-5
58-59	39.04848187367152	0.0	0.0	4.0545664084274834E-4	4.296229306943029E-5
60-61	40.10613297474139	5.370286633678786E-6	0.0	4.2156750074378473E-4	4.296229306943029E-5
62-63	41.125590597272534	5.370286633678786E-6	0.0	4.51104077229018E-4	4.296229306943029E-5
64-65	42.085583035908954	1.611085990103636E-5	0.0	4.8064065371425136E-4	4.296229306943029E-5
66-67	42.97269262949641	2.1481146534715144E-5	0.0	5.477692366352362E-4	4.296229306943029E-5
68-69	43.76992242083929	2.1481146534715144E-5	0.0	5.907315297046665E-4	4.564743638626968E-5
70-71	44.506913707012195	2.1481146534715144E-5	0.0	5.961018163383453E-4	4.8332579703109074E-5
72-73	45.204849583641675	2.1481146534715144E-5	0.0	6.041572462888635E-4	4.8332579703109074E-5
74-75	45.87422896109657	2.1481146534715144E-5	0.0	6.175829628730603E-4	4.8332579703109074E-5
76-77	46.52390207174918	2.1481146534715144E-5	0.0	6.820264024772058E-4	4.8332579703109074E-5
78-79	47.15118108713934	2.1481146534715144E-5	0.0	7.491549853981907E-4	4.8332579703109074E-5
80-81	47.76502901565868	2.1481146534715144E-5	0.0	8.377647148538907E-4	4.8332579703109074E-5
82-83	48.366917315844816	2.4166289851554537E-5	0.0	8.67301291339124E-4	4.8332579703109074E-5
84-85	48.96389448890445	2.685143316839393E-5	0.0	8.860972945569996E-4	4.8332579703109074E-5
86-87	49.55385538247718	2.685143316839393E-5	0.0	9.02208154458036E-4	4.8332579703109074E-5
88	49.994516937347015	2.685143316839393E-5	0.0	9.02208154458036E-4	4.8332579703109074E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTGCT	3385	0.0	53.310314	7
TTGCTGA	16215	0.0	51.41453	9
GTTGCTG	3520	0.0	46.19344	8
GCCTGCT	2975	0.0	44.38723	7
CTTGCTG	10420	0.0	43.47666	8
TGCTTGC	1655	0.0	43.15994	6
CCTGCTG	8720	0.0	42.25192	8
ACTTGCT	2370	0.0	42.234753	7
CTGCTGA	12380	0.0	41.83215	9
ATTGCTG	2470	0.0	40.335346	8
TACCCGA	13780	0.0	37.751446	6
TTTGCTG	4260	0.0	37.617645	8
ACCCGAC	15245	0.0	35.29529	7
CTACCCG	14285	0.0	33.390263	5
CGCTTGC	2500	0.0	31.95539	6
CGTTGCT	2020	0.0	31.406614	7
CCCGACG	18735	0.0	31.229319	8
AGTTGCT	1225	0.0	30.689695	7
ATGCTGA	3570	0.0	29.881805	9
GACACGC	1510	0.0	29.876406	2
>>END_MODULE