##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248488_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21329965
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.487982422849733	31.0	30.0	33.0	23.0	34.0
2	29.980134660324104	31.0	30.0	34.0	25.0	34.0
3	30.304853008432033	31.0	30.0	34.0	26.0	34.0
4	33.728033027714766	35.0	35.0	37.0	28.0	37.0
5	33.50681874067773	35.0	35.0	37.0	28.0	37.0
6	33.30193350059412	35.0	33.0	37.0	27.0	37.0
7	33.3753313237973	35.0	33.0	37.0	27.0	37.0
8	33.366564455215936	35.0	33.0	37.0	27.0	37.0
9	34.627968540970414	37.0	34.0	39.0	27.0	39.0
10-11	35.104783317741024	37.0	34.5	39.0	28.0	39.0
12-13	35.33925944088516	38.0	35.0	39.0	30.0	39.0
14-15	36.142500702650004	39.0	35.5	40.0	29.5	41.0
16-17	35.83194688786409	38.0	34.0	40.0	27.5	41.0
18-19	35.39148460862454	38.0	34.0	40.0	27.0	41.0
20-21	34.94737455030986	37.0	33.5	40.0	27.0	41.0
22-23	34.5777351486512	37.0	33.0	39.0	26.0	41.0
24-25	34.24531195902103	36.5	33.0	39.0	25.0	41.0
26-27	33.958407245393985	36.0	32.0	39.0	25.0	40.0
28-29	33.77133330973585	36.0	32.0	39.0	25.0	40.0
30-31	33.629526536963375	35.0	32.0	39.0	25.0	40.0
32-33	33.47019601297986	35.0	31.0	39.0	24.0	40.0
34-35	33.25874282962958	35.0	31.0	39.0	23.5	40.0
36-37	32.99389302795387	35.0	30.0	39.0	23.0	40.0
38-39	32.69717432729027	35.0	30.0	38.0	22.0	40.0
40-41	32.37739307120289	35.0	30.0	38.0	22.0	40.0
42-43	32.057143811534615	35.0	28.5	38.0	21.5	40.0
44-45	31.660140276835897	34.5	28.0	37.5	20.5	39.5
46-47	31.49722240519382	34.0	28.0	37.0	20.5	39.0
48-49	31.508249193095253	34.0	28.0	37.0	21.0	39.0
50-51	31.351651561547335	34.0	28.0	37.0	21.0	39.0
52-53	31.226380727769595	34.0	28.0	36.0	21.0	39.0
54-55	30.994207890167658	33.5	28.0	36.0	20.0	39.0
56-57	31.247606876054412	34.0	29.0	35.0	21.5	39.0
58-59	31.365813867955247	34.0	29.0	35.0	22.0	38.5
60-61	31.165551349943613	34.0	29.0	35.0	22.0	37.0
62-63	30.937090145248714	34.0	29.0	35.0	22.0	36.5
64-65	30.765944154151214	33.0	29.0	35.0	22.0	36.0
66-67	30.620820732711	33.0	29.0	35.0	21.0	35.0
68-69	30.51091645016764	33.0	29.0	35.0	20.5	35.0
70-71	30.43300192944527	33.0	29.0	35.0	20.0	35.0
72-73	30.373170138816448	33.0	29.0	35.0	20.0	35.0
74-75	30.29697875734911	33.0	29.0	35.0	20.0	35.0
76-77	30.138558642735703	33.0	29.0	35.0	20.0	35.0
78-79	29.953993079688598	33.0	28.5	35.0	19.5	35.0
80-81	29.77899295662229	33.0	28.0	35.0	18.0	35.0
82-83	29.562620707535153	33.0	28.0	35.0	16.0	35.0
84-85	29.34233415291586	33.0	27.5	35.0	15.0	35.0
86-87	28.998497278359338	33.0	27.0	35.0	10.5	35.0
88-89	28.61082216965663	33.0	26.5	35.0	10.0	35.0
90-91	28.101086968497135	33.0	25.0	35.0	8.0	35.0
92-93	27.57141994841529	33.0	24.5	35.0	7.5	35.0
94-95	27.103881745703756	33.0	23.5	35.0	7.0	35.0
96-97	26.546719298414224	32.0	20.0	35.0	7.0	35.0
98-99	25.9083851520619	31.5	18.5	35.0	7.0	35.0
100	24.176668972499485	29.0	13.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.258667531099654
1101	2	0.2922610034938202
1101	3	0.37797899291610193
1101	4	0.26121179183385834
1101	5	0.20969199007487305
1101	6	0.12219569690095966
1101	7	0.26520077365457695
1101	8	0.280097999638933
1101	9	0.43702795053609833
1101	10-11	0.5929508911896804
1101	12-13	0.6554357736962899
1101	14-15	0.8519378299065323
1101	16-17	0.8829050267247496
1101	18-19	0.8279777673633077
1101	20-21	0.7781667829644121
1101	22-23	0.806407420298271
1101	24-25	0.8162142224040991
1101	26-27	0.8277652521673389
1101	28-29	0.870869216095933
1101	30-31	0.827430593478887
1101	32-33	0.8540356671261335
1101	34-35	0.9268453444466473
1101	36-37	0.9208554162936693
1101	38-39	0.8281933470364251
1101	40-41	0.8824148133336678
1101	42-43	0.9006967551151845
1101	44-45	0.8314633713729407
1101	46-47	0.8870701506152514
1101	48-49	0.9268374836257607
1101	50-51	0.8586891630568942
1101	52-53	0.7962695352751261
1101	54-55	0.7584426980146404
1101	56-57	0.7329518498785106
1101	58-59	0.7144100888474014
1101	60-61	0.7102472355065146
1101	62-63	0.6553005350715821
1101	64-65	0.6560063318705041
1101	66-67	0.6719035198123606
1101	68-69	0.7102371168991866
1101	70-71	0.7026714627200654
1101	72-73	0.6895327341858213
1101	74-75	0.6172665056764117
1101	76-77	0.6365374468527065
1101	78-79	0.639261682840548
1101	80-81	0.6754954913314926
1101	82-83	0.7331669642695005
1101	84-85	0.7310481926648471
1101	86-87	0.6604557789513628
1101	88-89	0.6448969831174516
1101	90-91	0.5893416067739281
1101	92-93	0.49348717911275486
1101	94-95	0.49310373234946425
1101	96-97	0.4622117617202832
1101	98-99	0.46583937171131495
1101	100	0.5101003226155854
1102	1	0.2457235150366266
1102	2	0.29507063184910365
1102	3	0.3267443361293658
1102	4	0.277935890182718
1102	5	0.12210833405882937
1102	6	0.04775622626545584
1102	7	0.1729010115921028
1102	8	0.21259447914773233
1102	9	0.2206459694301941
1102	10-11	0.36438772276910214
1102	12-13	0.49735806750959455
1102	14-15	0.5689039254228021
1102	16-17	0.5890443896047017
1102	18-19	0.561134522538616
1102	20-21	0.5230433188036088
1102	22-23	0.5526627035648488
1102	24-25	0.5389818230576395
1102	26-27	0.504556644928428
1102	28-29	0.5459888267845585
1102	30-31	0.5123210797348392
1102	32-33	0.5103127949568034
1102	34-35	0.5698286668113184
1102	36-37	0.575507457179306
1102	38-39	0.5123518089210819
1102	40-41	0.5289695530664957
1102	42-43	0.5157362669606442
1102	44-45	0.4277859623330329
1102	46-47	0.5577708279738189
1102	48-49	0.5922376287333648
1102	50-51	0.6176164374814164
1102	52-53	0.5995870484808385
1102	54-55	0.5707415556843394
1102	56-57	0.5835485473649449
1102	58-59	0.6143664028814158
1102	60-61	0.609391272010086
1102	62-63	0.6168519456270758
1102	64-65	0.5721236345234608
1102	66-67	0.591344043464634
1102	68-69	0.6008426274719163
1102	70-71	0.6331275024637648
1102	72-73	0.5943404647076385
1102	74-75	0.5273672993032115
1102	76-77	0.5095652343525359
1102	78-79	0.552681727625064
1102	80-81	0.5479812136257358
1102	82-83	0.5857377671565231
1102	84-85	0.5605039139105017
1102	86-87	0.5659287719488297
1102	88-89	0.5190438460065714
1102	90-91	0.5049773006651783
1102	92-93	0.5006292941295101
1102	94-95	0.5260080645426726
1102	96-97	0.49074933519271013
1102	98-99	0.44373382704993247
1102	100	0.4517467753657449
1103	1	0.3187771200469065
1103	2	0.3305877658208338
1103	3	0.32359631336211336
1103	4	0.2866522755695371
1103	5	0.2589548960193824
1103	6	0.20097210983246327
1103	7	0.26163004123356615
1103	8	0.3477208562938614
1103	9	0.2692838541354021
1103	10-11	0.3432534492396897
1103	12-13	0.5078145611691554
1103	14-15	0.5704800531025072
1103	16-17	0.58516633551654
1103	18-19	0.5827954979360186
1103	20-21	0.5740209905431399
1103	22-23	0.5199093311686838
1103	24-25	0.5258799011261175
1103	26-27	0.5071301600943485
1103	28-29	0.5332867298737582
1103	30-31	0.4768671793838948
1103	32-33	0.5461187727710097
1103	34-35	0.5610063811377373
1103	36-37	0.5484052144659728
1103	38-39	0.48085905621884706
1103	40-41	0.4756234480139767
1103	42-43	0.5302469344706893
1103	44-45	0.4822000702995055
1103	46-47	0.4539256344726468
1103	48-49	0.5175168258572
1103	50-51	0.6188969586692998
1103	52-53	0.5653017317992841
1103	54-55	0.5515170188302143
1103	56-57	0.527305433630616
1103	58-59	0.5282874419288675
1103	60-61	0.5279297841541677
1103	62-63	0.5257946982343853
1103	64-65	0.5196909115787136
1103	66-67	0.522447201315444
1103	68-69	0.5562041790495407
1103	70-71	0.5438204028387084
1103	72-73	0.5679163473695255
1103	74-75	0.5677111394891448
1103	76-77	0.5678966396006366
1103	78-79	0.5816154432385225
1103	80-81	0.5851280012430529
1103	82-83	0.5820040741423753
1103	84-85	0.5486505283049752
1103	86-87	0.6347453634384266
1103	88-89	0.6009020369754801
1103	90-91	0.5384337167098074
1103	92-93	0.5058752466105645
1103	94-95	0.4294601561366207
1103	96-97	0.4453835172927967
1103	98-99	0.376886733112503
1103	100	0.2801480329046093
1104	1	0.14510157983629313
1104	2	0.161071583239071
1104	3	0.1999783857523525
1104	4	0.15182766664989344
1104	5	0.07342387292455044
1104	6	0.12341432177950651
1104	7	0.1488029399331836
1104	8	0.14525654165315416
1104	9	0.1756737451379209
1104	10-11	0.18778328503719166
1104	12-13	0.23665167963005018
1104	14-15	0.30525690371359815
1104	16-17	0.26093396799898727
1104	18-19	0.3365660529006931
1104	20-21	0.31621962270700976
1104	22-23	0.3098077325200066
1104	24-25	0.3204195559032712
1104	26-27	0.2933149080386528
1104	28-29	0.26201379927790214
1104	30-31	0.26330342697182374
1104	32-33	0.3119716420885865
1104	34-35	0.3389656074923977
1104	36-37	0.3466197121457171
1104	38-39	0.32026555620457486
1104	40-41	0.2688530093215249
1104	42-43	0.3447291424828123
1104	44-45	0.29704571629488186
1104	46-47	0.32259372253727747
1104	48-49	0.34160809535747916
1104	50-51	0.35039321214838637
1104	52-53	0.3211305189872071
1104	54-55	0.3433377421176793
1104	56-57	0.3282535281356047
1104	58-59	0.3481249180624246
1104	60-61	0.3752898330992487
1104	62-63	0.35006949775320706
1104	64-65	0.3541613314236791
1104	66-67	0.30297472115514523
1104	68-69	0.3618080569692985
1104	70-71	0.3448625198009374
1104	72-73	0.3458577308064257
1104	74-75	0.3282943688670841
1104	76-77	0.3138608130558822
1104	78-79	0.35923577334995827
1104	80-81	0.3485874733164387
1104	82-83	0.3863102709359687
1104	84-85	0.3914784410501255
1104	86-87	0.35882410351764804
1104	88-89	0.3895642262890249
1104	90-91	0.37439142660081615
1104	92-93	0.3543926055260158
1104	94-95	0.323757058226569
1104	96-97	0.3269661582872203
1104	98-99	0.295718910352317
1104	100	0.36900593488076794
1105	1	0.16751951015200817
1105	2	0.20347415698640603
1105	3	0.24604308802551245
1105	4	0.25259401664037284
1105	5	0.16514974223282763
1105	6	0.17752444357937947
1105	7	0.1931350777709966
1105	8	0.20715660963335836
1105	9	0.1481346925190934
1105	10-11	0.26333250199425606
1105	12-13	0.32472914391621543
1105	14-15	0.3797342461989146
1105	16-17	0.4081938656010706
1105	18-19	0.3957027762991032
1105	20-21	0.3536638642473946
1105	22-23	0.32439412606398577
1105	24-25	0.35487620248366625
1105	26-27	0.3428910993250156
1105	28-29	0.3336338214172514
1105	30-31	0.3332207061419652
1105	32-33	0.3217772090286317
1105	34-35	0.3995395254652081
1105	36-37	0.40799398830457534
1105	38-39	0.4033045645869322
1105	40-41	0.34974920362416384
1105	42-43	0.38149474382889537
1105	44-45	0.3799542140987384
1105	46-47	0.3406154494101621
1105	48-49	0.39305360254784816
1105	50-51	0.41111367358672624
1105	52-53	0.43473202514827136
1105	54-55	0.4578331790788859
1105	56-57	0.46447584592026914
1105	58-59	0.3747089139702666
1105	60-61	0.4411683685608807
1105	62-63	0.4566257171921819
1105	64-65	0.43624095637415294
1105	66-67	0.3965960889015214
1105	68-69	0.4364217525348195
1105	70-71	0.4791594134548909
1105	72-73	0.43591553069739675
1105	74-75	0.45813124444205755
1105	76-77	0.3979358234892132
1105	78-79	0.4408320636844323
1105	80-81	0.38548712259195383
1105	82-83	0.4277743562765046
1105	84-85	0.3861935916708248
1105	86-87	0.35595148674779153
1105	88-89	0.41486797257158514
1105	90-91	0.3744820951587897
1105	92-93	0.31302759563940086
1105	94-95	0.34869357394068246
1105	96-97	0.28807084153542206
1105	98-99	0.28060719903221454
1105	100	0.30108455703511083
1106	1	0.024216177671132044
1106	2	0.05944468910564282
1106	3	0.0856070715260202
1106	4	0.06045337481910451
1106	5	0.008612456650851641
1106	6	-0.009784586477486812
1106	7	0.08017501918838121
1106	8	0.07944020263953178
1106	9	0.01844677850500176
1106	10-11	0.11382231404318333
1106	12-13	0.16550658727073397
1106	14-15	0.18179185421286093
1106	16-17	0.18537700142847058
1106	18-19	0.12022384993714752
1106	20-21	0.13383242642604642
1106	22-23	0.10681463283624737
1106	24-25	0.149102792984678
1106	26-27	0.09911588511452862
1106	28-29	0.123809179509756
1106	30-31	0.13785187815143018
1106	32-33	0.16955325315616676
1106	34-35	0.28089754905424513
1106	36-37	0.26965758589910394
1106	38-39	0.2930948765792891
1106	40-41	0.28522383646636484
1106	42-43	0.23335909446444703
1106	44-45	0.23052874805945578
1106	46-47	0.21292450459714019
1106	48-49	0.17584852246930893
1106	50-51	0.27633428065152543
1106	52-53	0.3090177845346709
1106	54-55	0.2787650190961024
1106	56-57	0.29081105197629853
1106	58-59	0.2982713149784004
1106	60-61	0.321569814994767
1106	62-63	0.30572187147146934
1106	64-65	0.27547001134861304
1106	66-67	0.24264942340961682
1106	68-69	0.2711337516566026
1106	70-71	0.30182524602298955
1106	72-73	0.28284700810299057
1106	74-75	0.24288440940425104
1106	76-77	0.25387398674448747
1106	78-79	0.28178725856005826
1106	80-81	0.2617239190204721
1106	82-83	0.2604267074582154
1106	84-85	0.26107701743584855
1106	86-87	0.27255535476421144
1106	88-89	0.2575923739358714
1106	90-91	0.23584713320763484
1106	92-93	0.15925049183255524
1106	94-95	0.19381710634891647
1106	96-97	0.21126024885661465
1106	98-99	0.17438190572088885
1106	100	0.15419316421889562
1107	1	0.09095866074843428
1107	2	0.14957013313024348
1107	3	0.14526564292994593
1107	4	0.0985249938656736
1107	5	0.11563368797727946
1107	6	0.06911139382262377
1107	7	0.16522597195215383
1107	8	0.1218691714608795
1107	9	-0.02677249871933185
1107	10-11	0.17710293982924696
1107	12-13	0.22421365626413348
1107	14-15	0.33412847249681477
1107	16-17	0.3184094476610184
1107	18-19	0.2364286646664766
1107	20-21	0.24308723925245346
1107	22-23	0.23643455930289292
1107	24-25	0.2843993018214235
1107	26-27	0.27141728086620986
1107	28-29	0.36250097718506424
1107	30-31	0.3199047590450661
1107	32-33	0.33259266577634605
1107	34-35	0.3653247625603271
1107	36-37	0.4077260921821093
1107	38-39	0.3777555532973551
1107	40-41	0.378418925610454
1107	42-43	0.3767268099549028
1107	44-45	0.3097218409723723
1107	46-47	0.36615214562839427
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2216	40-41	0.08641022109969754
2216	42-43	0.1519537855046451
2216	44-45	0.1843003280892077
2216	46-47	0.10983355115359217
2216	48-49	0.05116466461240066
2216	50-51	-0.022767908080744803
2216	52-53	0.030484607619865045
2216	54-55	0.03693579196418639
2216	56-57	0.04488163536645473
2216	58-59	-0.027874260919126925
2216	60-61	0.01745359242011091
2216	62-63	-0.038869750651464585
2216	64-65	-0.049711132168535954
2216	66-67	-0.02038335213312692
2216	68-69	-0.04328338866150361
2216	70-71	-0.05540577062760477
2216	72-73	-0.11096648811775367
2216	74-75	-0.10217536217950496
2216	76-77	-0.2095102122014012
2216	78-79	-0.15782914156093142
2216	80-81	-0.2107547129502514
2216	82-83	-0.33890910268102914
2216	84-85	-0.3369319756338136
2216	86-87	-0.3370092099599056
2216	88-89	-0.44181246816064146
2216	90-91	-0.4780469891226602
2216	92-93	-0.40178637946443274
2216	94-95	-0.31786883460332405
2216	96-97	-0.355618268591094
2216	98-99	-0.3066082028571735
2216	100	-0.024454040321344905
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	851.0
6	5472.0
7	14468.0
8	30263.0
9	54650.0
10	79488.0
11	95844.0
12	99690.0
13	102167.0
14	107142.0
15	114604.0
16	121404.0
17	132497.0
18	147257.0
19	163689.0
20	182519.0
21	205505.0
22	229052.0
23	264099.0
24	353861.0
25	440150.0
26	425818.0
27	734955.0
28	634264.0
29	983039.0
30	1029606.0
31	1244744.0
32	1533537.0
33	2212653.0
34	2629717.0
35	4611607.0
36	1886174.0
37	404160.0
38	52990.0
39	2020.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.99653829261478	28.00306479894794	25.962406839634973	29.037990068802305
2	15.148424739524108	28.744999800983607	28.273577078487143	27.83299838100514
3	13.638606533109643	30.67273668756606	25.124457541303986	30.56419923802032
4	12.759168874320128	29.282127681140373	25.362899129267124	32.59580431527237
5	12.813115258276325	29.537952828333285	26.05690632872581	31.59202558466458
6	12.479013537575156	26.03543607255134	27.596289710653355	33.88926067922014
7	17.8868699027839	29.87132196522413	22.838378589907325	29.403429542084652
8	14.732560508186488	28.228133520144077	20.722321860349982	36.31698411131945
9	11.56062843984976	26.72623232152514	22.194813728011276	39.51832551061382
10-11	19.194770289838665	38.627978160798776	20.552045693976734	21.625205855385826
12-13	19.06648307827466	40.42182635913337	18.731180082064377	21.78051048052759
14-15	21.12211182440107	39.21381271750739	17.93662266118287	21.727452796908675
16-17	21.056154552492888	39.00548281526446	18.764270448672143	21.174092183570515
18-19	22.055583363383054	36.91094701666914	20.225204255870466	20.80826536407734
20-21	20.778019766716245	36.75769416486816	21.30296248047317	21.161323587942427
22-23	21.42801989033732	38.07084215601955	21.884061640073785	18.617076313569346
24-25	21.614927638184124	38.15059705911379	20.866208641223743	19.368266661478348
26-27	24.12143667371231	38.54187758864114	19.02102511654379	18.315660621102754
28-29	23.221948559221733	39.80028096623693	20.15808277228772	16.819687702253614
30-31	23.71521779831564	39.34098278857334	19.94982542956016	16.99397398355086
32-33	24.556710373577822	39.58988436921588	18.982492231584118	16.870913025622176
34-35	24.727637600565778	39.526677166771876	18.82577521882968	16.919910013832666
36-37	25.590642371201017	39.03567725609646	18.41972690632158	16.953953466380945
38-39	26.22188757378335	39.342073105353265	18.337883530060015	16.098155790803375
40-41	26.72849667233742	39.65678418474711	18.20491198351175	15.409807159403725
42-43	27.11400707541967	40.05097432064342	18.40948792695014	14.425530676986769
44-45	27.332904091743465	39.817526301205284	18.31613964239864	14.533429964652619
46-47	27.333548602983964	39.19078145813347	18.852365846669013	14.623304092213548
48-49	27.03984245486119	39.28858786971818	19.51554541635724	14.156024259063393
50-51	27.292541267648584	38.97241509772754	20.081784006677932	13.653259627945943
52-53	27.401010002580644	39.024119869410825	20.404906801098015	13.169963326910516
54-55	27.359138961425405	39.21377576205461	20.447814283341355	12.979270993178627
56-57	26.897931690354703	39.5254867502045	19.933105507151186	13.643476052289612
58-59	26.477548719428047	39.238929025495175	20.041848837898097	14.24167341717869
60-61	26.397878364367187	38.53978196910228	20.643012502813825	14.419327163716703
62-63	26.559526842933973	37.438719282285	21.15922168466213	14.8425321901189
64-65	26.202861701105473	37.06944636564888	21.314513585847457	15.41317834739819
66-67	25.429123597094357	36.677092237587125	21.970663565796546	15.923120599521976
68-69	24.903610260722	36.749179551195674	21.584311424389043	16.762898763693283
70-71	24.0633980599707	36.93255678402538	21.868924662207263	17.135120493796656
72-73	23.873116676687054	37.751148062564724	21.838362373702804	16.53737288704542
74-75	23.340544106403172	38.904088607487225	21.552949369336893	16.20241791677271
76-77	22.186156243007172	40.42671295664032	21.56472309818396	15.82240770216854
78-79	21.228734619462152	41.97824526881339	21.04662083750727	15.74639927421719
80-81	20.20215533370513	44.15179718699291	20.19357810632119	15.452469372980767
82-83	19.26473278468822	45.99820730338917	19.834330510469865	14.902729401452742
84-85	18.232111842124848	47.96055793541402	19.57928470692657	14.228045515534557
86-87	17.41568370431098	49.42893358624204	19.09911810993868	14.056264599508307
88-89	16.822144249081312	50.468741733214884	18.835833183757146	13.87328083394666
90-91	16.60951404448158	51.317264681737655	18.23183884653017	13.841382427250604
92-93	16.632891560413192	51.7570314262498	17.55714724536719	14.052929767969827
94-95	16.778839413329443	51.90558647118733	16.911433096882725	14.404141018600505
96-97	17.031153238463652	51.37979357632678	16.634720000697616	14.954333184511956
98-99	17.45022684708166	50.42599712445875	16.400624991282776	15.723151037176809
100	18.307987936301444	48.708760527594826	15.967639980483622	17.015611555620108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1264051.0
1	1290472.0
2	849899.0
3	238673.5
4	91734.5
5	86126.0
6	73453.0
7	52220.5
8	32811.5
9	22068.0
10	19125.5
11	20378.5
12	24102.5
13	29776.0
14	37796.5
15	48023.5
16	59294.5
17	71388.5
18	83945.5
19	96528.0
20	108300.5
21	118526.5
22	127146.0
23	133535.5
24	137990.5
25	141098.0
26	142003.0
27	140255.0
28	136741.5
29	131361.5
30	124756.5
31	116914.5
32	108793.0
33	102616.5
34	98262.5
35	95731.5
36	95938.5
37	100495.5
38	110198.5
39	126333.5
40	152519.0
41	192234.5
42	251817.5
43	339331.5
44	456930.0
45	600141.5
46	753933.0
47	893503.0
48	997858.0
49	1059996.0
50	1092090.0
51	1113739.5
52	1128711.0
53	1125039.0
54	1079141.5
55	972305.5
56	809297.5
57	615644.5
58	430114.5
59	279059.0
60	171769.5
61	105336.5
62	67294.0
63	46566.5
64	35165.0
65	28674.5
66	24446.0
67	21019.0
68	18223.0
69	15991.0
70	14197.5
71	12801.0
72	11601.5
73	10558.0
74	9758.5
75	9207.5
76	8698.5
77	8191.5
78	7757.0
79	7183.0
80	6631.5
81	6245.0
82	6030.5
83	5757.0
84	5457.5
85	5307.5
86	5177.0
87	5125.0
88	4952.0
89	4800.0
90	4667.5
91	4430.5
92	4184.5
93	3982.5
94	3837.5
95	3762.5
96	3589.0
97	3284.5
98	2948.0
99	2444.0
100	1866.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012320695322284868
2	3.000473746675159E-4
3	0.0
4	9.376480458359871E-6
5	0.0
6	0.003464609529363972
7	2.9067089420915603E-4
8	0.0
9	0.0
10-11	3.000473746675159E-4
12-13	1.6408840802129776E-5
14-15	4.008445395948845E-4
16-17	2.2034729077145696E-4
18-19	1.8518548905260745E-4
20-21	8.67324442398288E-5
22-23	4.688240229179935E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	1.7112076836506765E-4
32-33	2.3441201145899675E-5
34-35	9.37648045835987E-5
36-37	1.4767956721916796E-4
38-39	5.532123470432323E-4
40-41	0.0016572929210151072
42-43	9.634333670964768E-4
44-45	4.524151821158638E-4
46-47	5.977506292204418E-4
48-49	9.610892469818867E-4
50-51	0.0
52-53	9.142068446900874E-5
54-55	4.5007106200127377E-4
56-57	5.039858246368431E-4
58-59	4.758563832617634E-4
60-61	3.047356148966958E-5
62-63	2.344120114589968E-4
64-65	1.3830308676080809E-4
66-67	1.7815312870883755E-4
68-69	1.1017364538572848E-4
70-71	6.211918303663414E-4
72-73	5.485241068140524E-4
74-75	3.4927389707390516E-4
76-77	3.563062574176751E-4
78-79	7.876243585022291E-4
80-81	0.0010712628923676153
82-83	4.078768999386544E-4
84-85	6.962036740332204E-4
86-87	0.0012447277808472729
88-89	0.0011978453785554735
90-91	0.001101736453857285
92-93	7.173007550645301E-4
94-95	6.586977521997808E-4
96-97	6.751065930019107E-4
98-99	3.164562154696456E-4
100	8.813891630858278E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.1329965E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.05717682721382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.95294773852248	79.5568517649956
2	2.637193654337118	4.328013320430943
3	0.20530904125080698	0.5054124090642962
4	0.06409065044623961	0.21036391346552938
5	0.03526588972063702	0.1446909674387667
6	0.021657171007083854	0.1066275786543293
7	0.015714755974968145	0.09026559568841333
8	0.011913591784832095	0.07820765661881673
9	0.009425414173371104	0.06960805897446361
>10	0.0393647224310932	0.5793017439481004
>50	0.0031666287326440083	0.1846927057605273
>100	0.0024572746644917353	0.4065916049547431
>500	4.2629904538720505E-4	0.26293671454778833
>1k	5.879098202997268E-4	1.0483423058347077
>5k	1.7686905648582285E-4	1.0698136760651653
>10k+	3.023890320563853E-4	11.358279983557473
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	550558	2.581148164096847	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	150529	0.7057161134582265	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	134676	0.631393441105037	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124049	0.5815715121895418	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	92908	0.43557502321264946	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70231	0.3292597995355361	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67950	0.31856592357277663	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67294	0.3154904379824346	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64937	0.3044402557622575	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56449	0.26464647269697816	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55052	0.25809700109681377	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52728	0.24720153080419963	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49524	0.23218040910990712	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49064	0.23002381860448437	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46087	0.2160669274422157	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39385	0.18464634142625175	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38121	0.1787204057765683	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37605	0.17630127381831145	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37141	0.17412593035197196	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36340	0.17037064992839884	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33729	0.15812965469001003	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33602	0.1575342481809042	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30191	0.14154266075917143	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29741	0.13943295265604047	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26256	0.12309443545734837	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25223	0.11825148330060552	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24142	0.113183495612862	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24003	0.11253183022100599	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23630	0.11078311661552188	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23315	0.10930632094333019	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003342715283405294	0.0	0.0	4.688240229179936E-6	4.688240229179936E-6
2	0.008921721156129417	0.0	0.0	4.688240229179936E-6	4.688240229179936E-6
3	0.015377427951710187	0.0	0.0	9.376480458359871E-6	4.688240229179936E-6
4	0.025264926595050672	0.0	0.0	9.376480458359871E-6	4.688240229179936E-6
5	0.04175346748107651	0.0	0.0	9.376480458359871E-6	4.688240229179936E-6
6	0.07197855223859954	0.0	0.0	9.376480458359871E-6	9.376480458359871E-6
7	0.10676998297934384	0.0	0.0	9.376480458359871E-6	9.376480458359871E-6
8	0.22673267396360003	0.0	0.0	9.376480458359871E-6	9.376480458359871E-6
9	0.670685582465794	0.0	0.0	9.376480458359871E-6	9.376480458359871E-6
10-11	1.6811654402620912	0.0	0.0	9.376480458359871E-6	9.376480458359871E-6
12-13	3.057515565543591	0.0	0.0	1.1720600572949839E-5	9.376480458359871E-6
14-15	4.668315208205921	2.344120114589968E-6	0.0	1.6408840802129773E-5	9.376480458359871E-6
16-17	6.465034049516724	4.688240229179936E-6	0.0	2.5785321260489644E-5	9.376480458359871E-6
18-19	8.477334585406023	4.688240229179936E-6	0.0	2.8129441375079614E-5	9.376480458359871E-6
20-21	11.072802979282901	4.688240229179936E-6	0.0	2.8129441375079614E-5	9.376480458359871E-6
22-23	13.951344974077548	4.688240229179936E-6	9.376480458359871E-6	2.8129441375079614E-5	9.376480458359871E-6
24-25	16.87257574027899	4.688240229179936E-6	9.376480458359871E-6	2.8129441375079614E-5	1.1720600572949839E-5
26-27	19.73329538984241	4.688240229179936E-6	1.1720600572949839E-5	3.047356148966958E-5	1.4064720687539807E-5
28-29	22.679655123672262	4.688240229179936E-6	1.4064720687539807E-5	3.2817681604259546E-5	1.4064720687539807E-5
30-31	25.768127139449128	4.688240229179936E-6	1.4064720687539807E-5	3.2817681604259546E-5	1.4064720687539807E-5
32-33	29.21105824599337	4.688240229179936E-6	1.4064720687539807E-5	3.985004194802945E-5	1.4064720687539807E-5
34-35	32.349422514289174	4.688240229179936E-6	1.4064720687539807E-5	4.219416206261942E-5	1.4064720687539807E-5
36-37	34.8934374716508	4.688240229179936E-6	1.4064720687539807E-5	4.219416206261942E-5	1.4064720687539807E-5
38-39	36.849254089258935	4.688240229179936E-6	1.4064720687539807E-5	5.391476263556925E-5	1.4064720687539807E-5
40-41	38.512397934080056	4.688240229179936E-6	1.4064720687539807E-5	6.563536320851909E-5	1.4064720687539807E-5
42-43	39.857029770091046	4.688240229179936E-6	1.4064720687539807E-5	8.438832412523883E-5	1.4064720687539807E-5
44-45	40.95282622357796	4.688240229179936E-6	1.4064720687539807E-5	8.67324442398288E-5	1.4064720687539807E-5
46-47	41.8918643326419	4.688240229179936E-6	1.4064720687539807E-5	9.142068446900875E-5	1.4064720687539807E-5
48-49	42.753143289264656	4.688240229179936E-6	1.4064720687539807E-5	1.0782952527113852E-4	1.4064720687539807E-5
50-51	43.618498670766684	4.688240229179936E-6	1.4064720687539807E-5	1.2423836607326828E-4	1.4064720687539807E-5
52-53	44.535893518812614	4.688240229179936E-6	1.4064720687539807E-5	1.289266063024482E-4	1.4064720687539807E-5
54-55	45.498021211005266	4.688240229179936E-6	1.4064720687539807E-5	1.5471192756293787E-4	1.4064720687539807E-5
56-57	46.51917853592352	7.0323603437699034E-6	1.8752960916719742E-5	1.6643252813588772E-4	1.4064720687539807E-5
58-59	47.57197679414851	9.376480458359871E-6	2.3441201145899678E-5	1.7580900859424757E-4	1.4064720687539807E-5
60-61	48.63512434267942	9.376480458359871E-6	2.3441201145899678E-5	1.9221784939637735E-4	1.4064720687539807E-5
62-63	49.642730309215224	9.376480458359871E-6	2.3441201145899678E-5	2.1331493042768706E-4	1.4064720687539807E-5
64-65	50.56965400552697	9.376480458359871E-6	2.3441201145899678E-5	2.250355310006369E-4	1.4064720687539807E-5
66-67	51.39507964499707	9.376480458359871E-6	2.3441201145899678E-5	2.555090924903065E-4	1.4064720687539807E-5
68-69	52.105589484089634	9.376480458359871E-6	2.3441201145899678E-5	2.7191793329243627E-4	1.4064720687539807E-5
70-71	52.739158268661015	1.1720600572949839E-5	2.3441201145899678E-5	2.9535913443833593E-4	1.4064720687539807E-5
72-73	53.32085870745686	1.8752960916719742E-5	2.3441201145899678E-5	2.9535913443833593E-4	1.4064720687539807E-5
74-75	53.86541656303702	1.8752960916719742E-5	2.3441201145899678E-5	3.070797350112858E-4	1.6408840802129773E-5
76-77	54.379191433272396	1.8752960916719742E-5	2.3441201145899678E-5	3.445856568447252E-4	1.8752960916719742E-5
78-79	54.871737951750035	1.8752960916719742E-5	2.3441201145899678E-5	3.844356987927547E-4	1.8752960916719742E-5
80-81	55.347078628586594	1.8752960916719742E-5	2.3441201145899678E-5	4.2428574074078413E-4	1.8752960916719742E-5
82-83	55.816992667357866	1.8752960916719742E-5	2.3441201145899678E-5	4.4538282177209387E-4	1.8752960916719742E-5
84-85	56.28508532480011	1.8752960916719742E-5	2.3441201145899678E-5	4.500710620012738E-4	1.8752960916719742E-5
86-87	56.7556111789213	1.8752960916719742E-5	2.3441201145899678E-5	4.547593022304537E-4	1.8752960916719742E-5
88	57.10729951971323	1.8752960916719742E-5	2.3441201145899678E-5	4.641357826888136E-4	1.8752960916719742E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTGCT	3430	0.0	50.28806	7
TTGCTGA	15380	0.0	49.688877	9
GTTGCTG	3555	0.0	46.00803	8
GCCTGCT	3360	0.0	41.96381	7
CCTGCTG	10445	0.0	41.66741	8
CTGCTGA	13425	0.0	40.78558	9
CTTGCTG	11030	0.0	40.09665	8
TGCTTGC	1805	0.0	37.236256	6
TACCCGA	20115	0.0	36.264267	6
TTTGCTG	4385	0.0	34.512802	8
ACCCGAC	21860	0.0	34.314342	7
ATTGCTG	2220	0.0	34.085278	8
ACTTGCT	2440	0.0	32.93815	7
CCCGACG	25760	0.0	31.655384	8
TGACGCC	1795	0.0	31.421288	3
CTACCCG	21955	0.0	30.891577	5
TGAAAAA	12460	0.0	30.446472	1
GACACGC	1915	0.0	30.18871	2
CGCTTGC	2630	0.0	29.666037	6
GTACGCC	1225	0.0	29.543493	3
>>END_MODULE