##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248488_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21329965
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0054271068893	33.0	31.0	34.0	30.0	34.0
2	32.289707835901275	34.0	31.0	34.0	30.0	34.0
3	32.458846744474265	34.0	31.0	34.0	30.0	34.0
4	36.03695542866573	37.0	35.0	37.0	35.0	37.0
5	35.93621189720658	37.0	35.0	37.0	35.0	37.0
6	35.921221202191376	37.0	35.0	37.0	35.0	37.0
7	35.91853671583615	37.0	35.0	37.0	35.0	37.0
8	35.91457970043552	37.0	35.0	37.0	35.0	37.0
9	37.646542551757584	39.0	37.0	39.0	35.0	39.0
10-11	37.70291127997632	39.0	37.0	39.0	35.0	39.0
12-13	37.69069764061966	39.0	37.0	39.0	35.0	39.0
14-15	39.102544894002406	41.0	38.0	41.0	36.0	41.0
16-17	38.98344891798932	40.0	38.0	41.0	35.0	41.0
18-19	38.75963268106628	40.0	38.0	41.0	34.5	41.0
20-21	38.40032606710794	40.0	37.5	41.0	34.0	41.0
22-23	38.16556827449084	40.0	36.5	41.0	34.0	41.0
24-25	37.92703152583701	40.0	35.5	41.0	33.5	41.0
26-27	37.72478478047198	40.0	35.0	41.0	33.0	41.0
28-29	37.565550412295565	40.0	35.0	41.0	33.0	41.0
30-31	37.477021575047125	40.0	35.0	41.0	33.0	41.0
32-33	37.48235653457472	40.0	35.0	41.0	33.0	41.0
34-35	37.41916822179502	40.0	35.0	41.0	33.0	41.0
36-37	37.38156424541719	39.5	35.0	41.0	33.0	41.0
38-39	37.26942041395755	39.0	35.0	41.0	33.0	41.0
40-41	37.21439045961866	39.0	35.0	41.0	33.0	41.0
42-43	37.13334808566259	39.0	35.0	41.0	33.0	41.0
44-45	36.932608961149256	39.0	35.0	41.0	32.0	41.0
46-47	36.63939680632387	38.0	35.0	40.5	32.0	41.0
48-49	36.25300519714871	37.0	35.0	40.0	31.0	41.0
50-51	35.80847807298324	36.5	35.0	39.5	31.0	41.0
52-53	35.35064830157949	35.5	35.0	39.0	31.0	41.0
54-55	34.90455961835849	35.0	35.0	37.5	30.0	40.0
56-57	34.46327101333734	35.0	34.0	37.0	30.0	39.5
58-59	34.10909028683357	35.0	34.0	36.0	30.0	39.0
60-61	33.81337529152064	35.0	34.0	35.5	29.0	38.5
62-63	33.57032243137764	35.0	33.0	35.0	29.0	37.0
64-65	33.37315930898152	35.0	33.0	35.0	29.0	36.5
66-67	33.21045076726568	35.0	33.0	35.0	29.0	36.0
68-69	33.077282475615874	35.0	33.0	35.0	29.0	35.5
70-71	32.964124108970644	35.0	33.0	35.0	29.0	35.0
72-73	32.85785668190266	35.0	33.0	35.0	28.0	35.0
74-75	32.773524147836156	35.0	33.0	35.0	27.5	35.0
76-77	32.686571965776785	35.0	33.0	35.0	27.0	35.0
78-79	32.60559398480026	35.0	33.0	35.0	27.0	35.0
80-81	32.529835890494894	35.0	33.0	35.0	27.0	35.0
82-83	32.4355392284985	35.0	33.0	35.0	27.0	35.0
84-85	32.312778220686255	35.0	33.0	35.0	27.0	35.0
86-87	32.2112980729223	35.0	33.0	35.0	26.5	35.0
88-89	32.085829137553674	35.0	33.0	35.0	25.0	35.0
90-91	31.962600454337363	35.0	33.0	35.0	25.0	35.0
92-93	31.746464445675368	35.0	32.0	35.0	24.5	35.0
94-95	31.43284128689381	34.0	32.0	35.0	23.5	35.0
96-97	31.095582505644053	34.0	31.0	35.0	20.0	35.0
98-99	30.57625546971127	34.0	31.0	35.0	18.0	35.0
100	28.730036265882294	33.0	27.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.26605071502750377
1101	2	0.311318127207862
1101	3	0.27838465060322903
1101	4	0.18144413335456022
1101	5	0.24533928062837163
1101	6	0.22149296956977338
1101	7	0.2458697448014462
1101	8	0.24001019447283056
1101	9	0.31294019897129743
1101	10-11	0.308583229205766
1101	12-13	0.3177591838353422
1101	14-15	0.4417409778839527
1101	16-17	0.463167478503685
1101	18-19	0.50489633167291
1101	20-21	0.5313097777795974
1101	22-23	0.5132558522970001
1101	24-25	0.48657299082642425
1101	26-27	0.4962966915371041
1101	28-29	0.5498457075448755
1101	30-31	0.5567084217867091
1101	32-33	0.5516046179096818
1101	34-35	0.5431216686428755
1101	36-37	0.5402097371048882
1101	38-39	0.5488763694380481
1101	40-41	0.5259707883813718
1101	42-43	0.5108980570324135
1101	44-45	0.5009824155128868
1101	46-47	0.511683048070374
1101	48-49	0.48699108885595166
1101	50-51	0.47518282699327585
1101	52-53	0.469620947031828
1101	54-55	0.44687550062306514
1101	56-57	0.445387497179361
1101	58-59	0.45674379754687777
1101	60-61	0.4246370540713187
1101	62-63	0.43087411579325874
1101	64-65	0.4118266825409975
1101	66-67	0.4208892136378637
1101	68-69	0.4244880269760998
1101	70-71	0.4298872049068052
1101	72-73	0.4457605764334005
1101	74-75	0.44948851575902893
1101	76-77	0.44238096388994563
1101	78-79	0.47907309993749436
1101	80-81	0.46983823719013884
1101	82-83	0.46864767065420665
1101	84-85	0.49029380498461705
1101	86-87	0.5317357496446391
1101	88-89	0.4707794770741529
1101	90-91	0.4669278299094408
1101	92-93	0.46615003814835276
1101	94-95	0.4788834261169548
1101	96-97	0.5250300187875929
1101	98-99	0.7100253704499586
1101	100	1.1869141198856852
1102	1	0.25047613152568005
1102	2	0.2810302419096544
1102	3	0.275443691767272
1102	4	0.19041337856099716
1102	5	0.17012650736419488
1102	6	0.1747559769831355
1102	7	0.24506446840952378
1102	8	0.20069525745841332
1102	9	0.25061002274878774
1102	10-11	0.3004782255356986
1102	12-13	0.33490096112780066
1102	14-15	0.39567718049930534
1102	16-17	0.4475097654942104
1102	18-19	0.5041890320088669
1102	20-21	0.5332966825515868
1102	22-23	0.47948533254183445
1102	24-25	0.47392720618852735
1102	26-27	0.5138074857495525
1102	28-29	0.5228687346499683
1102	30-31	0.5449947245872835
1102	32-33	0.5241643176360995
1102	34-35	0.531736635369441
1102	36-37	0.5117644097988219
1102	38-39	0.5619727149702243
1102	40-41	0.5505387991612238
1102	42-43	0.5421476685546054
1102	44-45	0.54972638216951
1102	46-47	0.5325415660020738
1102	48-49	0.5311073623984655
1102	50-51	0.5183756446726235
1102	52-53	0.4802854575333413
1102	54-55	0.47636460977340533
1102	56-57	0.46693211783212973
1102	58-59	0.4435683283978591
1102	60-61	0.44909094882302725
1102	62-63	0.43290214577153563
1102	64-65	0.4030949998151456
1102	66-67	0.41501952550664356
1102	68-69	0.43946689707203035
1102	70-71	0.4505999473710318
1102	72-73	0.4350637380008351
1102	74-75	0.44480506841107115
1102	76-77	0.46163316450525826
1102	78-79	0.4511136718830784
1102	80-81	0.44140903653548946
1102	82-83	0.4296632069837898
1102	84-85	0.45237506701604957
1102	86-87	0.4283928982624303
1102	88-89	0.48432072693485395
1102	90-91	0.5436787482475829
1102	92-93	0.48276220049836027
1102	94-95	0.4542969657243603
1102	96-97	0.511819121474371
1102	98-99	0.6634601302742276
1102	100	1.0040879544237988
1103	1	0.2051764512915426
1103	2	0.21496184104301364
1103	3	0.2227404907494801
1103	4	0.14403673573362852
1103	5	0.17344911595013457
1103	6	0.15686521955500154
1103	7	0.20453015742125302
1103	8	0.2177196418587215
1103	9	0.23835225422035933
1103	10-11	0.2681799505898397
1103	12-13	0.2988143713582758
1103	14-15	0.3741144849418916
1103	16-17	0.4148714151254964
1103	18-19	0.42800907296609125
1103	20-21	0.45935265709755413
1103	22-23	0.43588295322381754
1103	24-25	0.4150243392620041
1103	26-27	0.42845492428077137
1103	28-29	0.4410561452608732
1103	30-31	0.47938617200333056
1103	32-33	0.4837342038060015
1103	34-35	0.45712160099387233
1103	36-37	0.42927135850116116
1103	38-39	0.46528479977675374
1103	40-41	0.461485964941005
1103	42-43	0.44801526487280086
1103	44-45	0.45723337718697366
1103	46-47	0.4627755257330435
1103	48-49	0.4433314768921264
1103	50-51	0.4293642684847754
1103	52-53	0.4183852326858215
1103	54-55	0.43973880913422647
1103	56-57	0.42641158206137675
1103	58-59	0.3947533747136589
1103	60-61	0.41312401793611286
1103	62-63	0.3926297782171133
1103	64-65	0.3683686921471647
1103	66-67	0.37314571604711233
1103	68-69	0.4006533306926201
1103	70-71	0.385477781363214
1103	72-73	0.40462931558188586
1103	74-75	0.41289973414214387
1103	76-77	0.3994090774967276
1103	78-79	0.38254016178201766
1103	80-81	0.3796501393053333
1103	82-83	0.3870204263555337
1103	84-85	0.3734298344184239
1103	86-87	0.42663051812538555
1103	88-89	0.45114898684637694
1103	90-91	0.45673383812004786
1103	92-93	0.4189350034803745
1103	94-95	0.3939750097851338
1103	96-97	0.432101870597851
1103	98-99	0.5975017370302744
1103	100	1.0009985064578082
1104	1	0.1627962805425014
1104	2	0.18191217451085606
1104	3	0.18454341063149116
1104	4	0.11531854540204023
1104	5	0.1252066196216859
1104	6	0.14381982376016111
1104	7	0.180847729181572
1104	8	0.1655125399929176
1104	9	0.2125853665899129
1104	10-11	0.20084323248369174
1104	12-13	0.20501091219293954
1104	14-15	0.2852345637970828
1104	16-17	0.30880377863579156
1104	18-19	0.28026636035652785
1104	20-21	0.3373700831677695
1104	22-23	0.3419134827941832
1104	24-25	0.3049738104025437
1104	26-27	0.3281369904999778
1104	28-29	0.3573721513894057
1104	30-31	0.3746608765689743
1104	32-33	0.37571745566083337
1104	34-35	0.3822872130970296
1104	36-37	0.35489790048558234
1104	38-39	0.3548960686736251
1104	40-41	0.3187580746857819
1104	42-43	0.31637169756892547
1104	44-45	0.31454338342874877
1104	46-47	0.3331292133791308
1104	48-49	0.302596793441829
1104	50-51	0.3065212777027
1104	52-53	0.30909124353922124
1104	54-55	0.3063099268788392
1104	56-57	0.3177423217279056
1104	58-59	0.2645751506948386
1104	60-61	0.29695194241241296
1104	62-63	0.26808083795994264
1104	64-65	0.25652869046570004
1104	66-67	0.2610521717121159
1104	68-69	0.25885456268796503
1104	70-71	0.2572025620314804
1104	72-73	0.24213602188164884
1104	74-75	0.2726738236543085
1104	76-77	0.3013965628713393
1104	78-79	0.29134691512258826
1104	80-81	0.25517422328471895
1104	82-83	0.2897078615167743
1104	84-85	0.2342867492576417
1104	86-87	0.2257160231128026
1104	88-89	0.2445987465241899
1104	90-91	0.22079370000797027
1104	92-93	0.23552452775280486
1104	94-95	0.1772644066531761
1104	96-97	0.22654163399863236
1104	98-99	0.4099880730786971
1104	100	0.8046266055388287
1105	1	0.15763734995756806
1105	2	0.19894344100654138
1105	3	0.18051009198975265
1105	4	0.12093291282932483
1105	5	0.11857302108078471
1105	6	0.1388576462845066
1105	7	0.1914843021785515
1105	8	0.1702592766073252
1105	9	0.21924969871078304
1105	10-11	0.20550515521039614
1105	12-13	0.21908412425949564
1105	14-15	0.2736543259886517
1105	16-17	0.30474228657579516
1105	18-19	0.3185975362973039
1105	20-21	0.3672816554681759
1105	22-23	0.35414566242119605
1105	24-25	0.35230413885201983
1105	26-27	0.3611283685525635
1105	28-29	0.3906178510065885
1105	30-31	0.3982156710533715
1105	32-33	0.3645318779690214
1105	34-35	0.3668868600780826
1105	36-37	0.3576016650942222
1105	38-39	0.35735204571168566
1105	40-41	0.34744898527008417
1105	42-43	0.3684803511872161
1105	44-45	0.38454205341442105
1105	46-47	0.35948622256730545
1105	48-49	0.3467148972111147
1105	50-51	0.33331026748830084
1105	52-53	0.3016217032628319
1105	54-55	0.29802710750936257
1105	56-57	0.30060425008983316
1105	58-59	0.29894378834381996
1105	60-61	0.28993883997568304
1105	62-63	0.29352774061250386
1105	64-65	0.261878626361451
1105	66-67	0.28764150591951676
1105	68-69	0.2879464568137067
1105	70-71	0.28208169710953257
1105	72-73	0.281572239588499
1105	74-75	0.23743202060853008
1105	76-77	0.2713942590538281
1105	78-79	0.25057792294364845
1105	80-81	0.24587221459803033
1105	82-83	0.23440888664762838
1105	84-85	0.23703430062037256
1105	86-87	0.27838469345439876
1105	88-89	0.30010783982706357
1105	90-91	0.3045050484388234
1105	92-93	0.26943785818117405
1105	94-95	0.2641882564669622
1105	96-97	0.2582187734884691
1105	98-99	0.39971040046912165
1105	100	0.866484127959275
1106	1	0.14204116363225694
1106	2	0.15813008638419035
1106	3	0.14154621162850134
1106	4	0.10453726486085202
1106	5	0.07433544383938795
1106	6	0.08378885867188046
1106	7	0.10930585154000028
1106	8	0.12949866858766512
1106	9	0.15948135749351877
1106	10-11	0.14307627026968817
1106	12-13	0.1478717862008736
1106	14-15	0.17812847288076483
1106	16-17	0.19098791843433816
1106	18-19	0.21767800024045414
1106	20-21	0.24664806076766865
1106	22-23	0.21276498286162138
1106	24-25	0.24242720456975775
1106	26-27	0.24434152580387547
1106	28-29	0.22801523802848322
1106	30-31	0.28547397188624757
1106	32-33	0.2792917507461752
1106	34-35	0.26460992044511045
1106	36-37	0.22172457790768618
1106	38-39	0.26718407743194916
1106	40-41	0.1810342716174631
1106	42-43	0.2124531486001473
1106	44-45	0.21328138066728286
1106	46-47	0.2002546930755429
1106	48-49	0.19192683085930895
1106	50-51	0.19486076110000283
1106	52-53	0.1869516589770015
1106	54-55	0.1690601460643606
1106	56-57	0.18153147251944546
1106	58-59	0.15794759037912343
1106	60-61	0.17588674859266717
1106	62-63	0.14978239885719802
1106	64-65	0.15685732811425623
1106	66-67	0.16863793763941715
1106	68-69	0.15806386534402606
1106	70-71	0.16203313301132027
1106	72-73	0.1452400902240214
1106	74-75	0.15733620909914947
1106	76-77	0.14799857219721702
1106	78-79	0.17683609493649044
1106	80-81	0.16429567950094537
1106	82-83	0.12545923325428276
1106	84-85	0.1856518144799182
1106	86-87	0.1528863299908707
1106	88-89	0.16174700012328458
1106	90-91	0.17684412243419345
1106	92-93	0.1728147238496014
1106	94-95	0.1840848925984453
1106	96-97	0.20532850276280712
1106	98-99	0.35278505145092254
1106	100	0.7511656425660505
1107	1	0.07950871234140777
1107	2	0.09466049657805797
1107	3	0.10754097947078378
1107	4	0.07737145148840341
1107	5	0.04950086990735514
1107	6	0.06160642369690805
1107	7	0.11682244378851436
1107	8	0.09826370979261867
1107	9	0.12053641282317784
1107	10-11	0.1336618826078393
1107	12-13	0.13090239341092058
1107	14-15	0.1609992149355577
1107	16-17	0.11766973348731824
1107	18-19	0.13995321320663834
1107	20-21	0.1856171054512714
1107	22-23	0.18633681320256557
1107	24-25	0.14616631580526018
1107	26-27	0.2227383026553582
1107	28-29	0.1849699043701989
1107	30-31	0.22935062842960008
1107	32-33	0.17274819709025024
1107	34-35	0.19788060530392215
1107	36-37	0.17404149605952313
1107	38-39	0.21290012357995636
1107	40-41	0.20139810061726138
1107	42-43	0.17988590668969096
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2216	54-55	-0.5684050472905682
2216	56-57	-0.55336215497627
2216	58-59	-0.5236244808953927
2216	60-61	-0.5138048440703997
2216	62-63	-0.5493667990035078
2216	64-65	-0.5393693012848004
2216	66-67	-0.5123326236529735
2216	68-69	-0.5319914094481817
2216	70-71	-0.5314682524099652
2216	72-73	-0.563791692062523
2216	74-75	-0.5605678404367467
2216	76-77	-0.5619996302587182
2216	78-79	-0.6146249591548525
2216	80-81	-0.6275314920212516
2216	82-83	-0.634024298193097
2216	84-85	-0.7222796140221703
2216	86-87	-0.7470750533050392
2216	88-89	-0.8008197171265437
2216	90-91	-0.7994497262181319
2216	92-93	-0.8613932454906852
2216	94-95	-0.7935633922895029
2216	96-97	-0.8421197960854592
2216	98-99	-0.6943486503005438
2216	100	-0.1313640254189643
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	6.0
7	27.0
8	66.0
9	224.0
10	478.0
11	990.0
12	1670.0
13	2751.0
14	4647.0
15	8731.0
16	16490.0
17	26910.0
18	39100.0
19	52219.0
20	66316.0
21	82377.0
22	101599.0
23	123275.0
24	149552.0
25	180706.0
26	217177.0
27	260198.0
28	318948.0
29	399829.0
30	466472.0
31	577476.0
32	746150.0
33	1046463.0
34	1453311.0
35	4250020.0
36	3837144.0
37	5816219.0
38	959521.0
39	122685.0
40	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.88027293947297	22.863482435335833	28.892069619072004	27.364175006119197
2	18.71220769456935	23.900704909635422	34.490784270338395	22.896303125456832
3	15.875905094077744	21.629805768551424	35.80684731550192	26.68744182186891
4	16.534943352153363	18.354437708840866	38.42841167322717	26.682207265778608
5	15.025940148652857	20.580508728946786	40.5270854359971	23.866465686403256
6	13.3930755259967	14.639842409033548	36.99385770483143	34.973224360138325
7	18.649847761119158	15.308928073721638	35.075425580867105	30.9657985842921
8	14.226404028323532	14.472119386975086	35.14224706885361	36.15922951584778
9	13.165516211583094	13.144480077674764	40.71887600378153	32.97112770696061
10-11	17.68928359704294	18.73948222606085	40.72528951641505	22.84594466048116
12-13	16.053314939020822	29.317194365071202	32.294006387266556	22.33548430864142
14-15	18.874003849964602	26.618933462898504	31.33456227087667	23.17250041626022
16-17	27.13374139467565	27.293298633476297	31.76042077522591	13.812539196622142
18-19	27.010258268362332	19.598070592194375	31.113525763251975	22.27814537619132
20-21	19.400778266120525	18.581599997618362	38.54239156528546	23.475230170975646
22-23	19.14649672510467	20.77876151168865	40.48255256649346	19.592189196713225
24-25	19.582465936977357	23.027706244398136	37.37803658073324	20.01179123789127
26-27	24.848734183001433	23.009959677920328	32.191916783816595	19.949389355261644
28-29	22.520969737977435	26.46335971143555	35.64212913049031	15.373541420096704
30-31	22.82323951378033	23.48897921990255	35.613262602777496	18.074518663539624
32-33	23.855793528692136	24.787842890267495	34.629212096113484	16.727151484926882
34-35	23.892155898171332	24.86644035187447	34.650324986138095	16.591078763816103
36-37	24.299558314346744	23.434760042278914	34.22259637612741	18.04308526724693
38-39	24.89622568022148	23.4988820805812	33.16803470823392	18.436857530963398
40-41	24.897194027902614	23.981027562282176	33.37023647846711	17.7515419313481
42-43	24.893502622658644	24.796716234556364	33.443134908461644	16.866646234323348
44-45	25.338658897194012	23.72170374190774	32.86311632878821	18.076521032110048
46-47	25.963629845355975	22.894680897353233	33.34536244213556	17.796326815155243
48-49	24.631710594715038	22.887055305796768	33.75211464719545	18.72911945229275
50-51	24.434880324185073	22.46566838849744	34.004409346482994	19.09504194083449
52-53	23.78417138454504	23.041729532707052	34.23999753954803	18.934101543199887
54-55	23.302096973816415	23.45476177226189	34.216300932355615	19.026840321566084
56-57	22.76366950190805	23.87739298947391	33.35298359078867	20.00595391782937
58-59	22.261999271335803	23.945034471367535	34.177400617297	19.61556563999966
60-61	21.257382604289315	23.17618803550974	34.81912100782328	20.747308352377665
62-63	20.911109463254764	22.893367333147456	35.12294283428223	21.072580369315553
64-65	20.7555398880827	22.911803581215764	34.93253615831091	21.400120372390628
66-67	19.805732149963575	22.683315740642517	35.551900391571685	21.959051717822227
68-69	19.609373761895068	22.2001023257829	36.078102273082116	22.112421639239912
70-71	18.93151358359581	21.570199933585606	37.061114957738475	22.437171525080107
72-73	18.599149760387487	21.09012780881222	38.10917776898112	22.20154466181917
74-75	18.48509328376184	20.472341325407296	38.808183778341935	22.234381612488928
76-77	18.45519812488177	20.206332860678145	38.957782413429435	22.380686601010648
78-79	18.467602472954724	20.248886350319374	39.0814975369994	22.202013639726502
80-81	18.33633731135274	19.646180345345144	39.66444215618302	22.353040187119095
82-83	18.499241683799784	19.448256948797614	40.19733921072052	21.855162156682077
84-85	18.412925871002916	19.546349307385555	40.47834948067749	21.562375340934047
86-87	18.519663226040002	19.795779159652653	40.51097899585251	21.173578618454837
88-89	18.40716605054648	20.635689653220144	40.396342274642414	20.56080202159096
90-91	18.45677260671981	21.553938259323687	40.15568849782155	19.833600636134953
92-93	18.153084728126952	22.51260853921365	39.540238782753526	19.794067949905866
94-95	18.01605413615391	23.8856214531238	38.92326773290415	19.175056677818134
96-97	17.088116400740613	25.61124984111857	38.95813824303937	18.342495515101437
98-99	16.55600879316864	27.22052066479866	38.37720067618169	17.84626986585101
100	15.993059691236427	28.734209652297405	37.60137985401683	17.67135080244934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	338689.0
1	754984.5
2	1154074.5
3	796142.5
4	278451.5
5	71153.0
6	32278.5
7	20692.0
8	16881.5
9	16539.5
10	18186.0
11	21584.5
12	26896.0
13	34619.5
14	44270.5
15	55389.5
16	68840.5
17	83845.0
18	98011.5
19	109726.5
20	117772.5
21	123074.0
22	127482.5
23	131414.0
24	134773.5
25	137415.5
26	138042.0
27	135637.0
28	131306.0
29	125513.0
30	118483.0
31	110301.0
32	101549.0
33	93029.5
34	84783.0
35	76346.0
36	68012.0
37	60810.5
38	54337.0
39	48695.5
40	44253.5
41	41990.5
42	44414.5
43	54353.0
44	79840.5
45	133591.0
46	224367.5
47	353490.0
48	514821.5
49	705530.0
50	931853.5
51	1198626.5
52	1486285.0
53	1730914.5
54	1847870.0
55	1772032.5
56	1503455.0
57	1119577.0
58	729265.5
59	418110.5
60	215131.5
61	101767.5
62	45391.5
63	19898.5
64	8919.5
65	4368.0
66	2465.5
67	1667.5
68	1230.5
69	920.5
70	712.5
71	539.0
72	430.5
73	336.5
74	248.5
75	189.0
76	128.5
77	87.0
78	63.5
79	34.0
80	24.0
81	21.0
82	12.5
83	7.5
84	6.5
85	5.5
86	4.5
87	4.5
88	6.0
89	5.5
90	2.5
91	0.5
92	1.0
93	1.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016994870830777265
2	0.10107845934111942
3	0.0
4	1.312707264170382E-4
5	0.0365917149887494
6	0.0014814839124208596
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.602920820545182E-4
14-15	7.430860763250198E-4
16-17	9.376480458359871E-6
18-19	2.344120114589968E-6
20-21	5.368035062411026E-4
22-23	5.76653548189132E-4
24-25	0.0026605763300596133
26-27	0.0021565905054227704
28-29	0.003080173830571217
30-31	0.003553686093718391
32-33	0.003673236219562479
34-35	0.003865454068958857
36-37	0.0030731414702274474
38-39	0.001812004848578045
40-41	0.003319274082259394
42-43	0.004857016877430413
44-45	0.004774972673419764
46-47	0.011875312500512776
48-49	0.0049343728412118825
50-51	0.006598698122570759
52-53	0.002580876246163554
54-55	0.0010689187722530253
56-57	0.005400852744015285
58-59	0.002456637880090286
60-61	0.013933449961122769
62-63	0.012158951034378163
64-65	0.009303812734807583
66-67	0.011497909162063792
68-69	0.00940929813996413
70-71	0.008239582202783736
72-73	0.005410229224473645
74-75	0.0036122890965831402
76-77	5.157064252097928E-4
78-79	0.009929692805403104
80-81	0.006106432898506866
82-83	0.009941413405976053
84-85	0.008406014730919625
86-87	0.00719410463167661
88-89	0.009425706980766259
90-91	0.005426638065275775
92-93	0.009184262608963493
94-95	0.002967656065070899
96-97	0.005466488107223805
98-99	0.44465145629634173
100	1.7539878757419434
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.1329965E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.40748617713174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.56094107825662	78.6078347607901
2	3.0163988610805643	4.91114897176264
3	0.23808025093613386	0.5814454421139412
4	0.06243928126894738	0.203320997072475
5	0.02975265133430934	0.1211044276115404
6	0.01752804480433397	0.0856148439072578
7	0.011221739080245001	0.06394734983408969
8	0.007822561296063941	0.05094520404632729
9	0.00709242932947553	0.05196391583414067
>10	0.03706939831758743	0.6041795867205306
>50	0.005289255029779345	0.3055337207754715
>100	0.004163927955379661	0.6611097302989979
>500	6.519048982556535E-4	0.37225980208667614
>1k	7.65906155363309E-4	1.4493891855269032
>5k	2.1869845420048958E-4	1.3065473183657208
>10k+	5.640118029380818E-4	10.62365474325309
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60375	0.28305250383673863	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47453	0.2224710635952755	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47172	0.22115366809087592	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46467	0.21784845872930406	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42472	0.19911893901373023	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41633	0.19518550546144825	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39228	0.18391028771027051	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38001	0.1781578169490667	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37857	0.17748271035606483	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37732	0.17689668032741732	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37318	0.17495574887253681	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36603	0.17160365710867317	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36587	0.1715286452650063	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35500	0.16643252813588771	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34049	0.15962989156334761	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34038	0.15957832092082663	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33891	0.1588891496071372	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32602	0.15284600795172426	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32356	0.151692700855346	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30962	0.14515729397586916	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30926	0.14498851732761867	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29020	0.13605273145080174	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29015	0.13602929024965582	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28933	0.1356448545508631	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28685	0.13448217097402645	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28632	0.1342336942418799	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28555	0.13387269974423305	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28265	0.13251311007777086	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27872	0.13067063166770315	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27578	0.12929228904032425	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27036	0.1267512628361087	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26823	0.1257526676672934	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26045	0.1221052167689914	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25917	0.12150512201965638	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25520	0.11964389064867194	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25368	0.11893127813383661	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25249	0.1183733775465642	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25234	0.11830305394312648	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25219	0.11823273033968879	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24690	0.11575265125845262	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24307	0.11395705525067668	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24260	0.11373670795990524	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23467	0.11001893345816555	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23044	0.10803580784122242	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22611	0.10600579982198752	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22526	0.10560729940250722	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22418	0.1051009694577558	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22319	0.10463683367506699	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22268	0.1043977334233788	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21934	0.10283186118683271	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21900	0.1026724610190406	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21827	0.10233021948231046	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21749	0.10196453674443441	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003047356148966958	0.0	0.0	1.4064720687539807E-5	0.0
2	0.007852802383876391	0.0	0.0	1.4064720687539807E-5	0.0
3	0.013314602250871017	0.0	0.0	1.4064720687539807E-5	0.0
4	0.022362905893188293	0.0	0.0	1.4064720687539807E-5	0.0
5	0.038879576220589206	0.0	0.0	1.8752960916719742E-5	0.0
6	0.0728318119603103	0.0	0.0	1.8752960916719742E-5	0.0
7	0.11195517667281686	0.0	0.0	1.8752960916719742E-5	0.0
8	0.28481528216291024	0.0	0.0	1.8752960916719742E-5	0.0
9	0.9691389554553888	0.0	0.0	1.8752960916719742E-5	0.0
10-11	2.3395420480061735	0.0	0.0	1.8752960916719742E-5	0.0
12-13	3.870517368406371	0.0	0.0	2.3441201145899678E-5	0.0
14-15	5.546596068019802	0.0	0.0	2.5785321260489644E-5	0.0
16-17	7.268708598443551	0.0	0.0	3.047356148966958E-5	0.0
18-19	9.15992126569359	0.0	0.0	3.2817681604259546E-5	4.688240229179936E-6
20-21	11.61228581481498	0.0	0.0	3.7505921833439485E-5	4.688240229179936E-6
22-23	14.44207245534627	0.0	0.0	3.985004194802945E-5	4.688240229179936E-6
24-25	17.573146510085692	0.0	0.0	4.219416206261942E-5	4.688240229179936E-6
26-27	20.783862045718312	0.0	0.0	4.453828217720939E-5	4.688240229179936E-6
28-29	24.20130553425662	0.0	0.0	4.6882402291799356E-5	4.688240229179936E-6
30-31	27.72324520926312	0.0	0.0	4.6882402291799356E-5	4.688240229179936E-6
32-33	31.667039772451574	2.344120114589968E-6	0.0	4.6882402291799356E-5	4.688240229179936E-6
34-35	35.32401951901937	4.688240229179936E-6	0.0	4.6882402291799356E-5	4.688240229179936E-6
36-37	38.164197644018635	4.688240229179936E-6	0.0	5.391476263556926E-5	4.688240229179936E-6
38-39	40.25597322827299	4.688240229179936E-6	0.0	6.329124309392913E-5	4.688240229179936E-6
40-41	42.14939640079109	4.688240229179936E-6	0.0	6.563536320851909E-5	4.688240229179936E-6
42-43	43.682804917870236	4.688240229179936E-6	0.0	7.735596378146893E-5	4.688240229179936E-6
44-45	44.90457438631522	4.688240229179936E-6	0.0	7.97000838960589E-5	4.688240229179936E-6
46-47	45.91839695939492	4.688240229179936E-6	0.0	7.97000838960589E-5	4.688240229179936E-6
48-49	46.81224980913002	4.688240229179936E-6	0.0	8.67324442398288E-5	4.688240229179936E-6
50-51	47.67375145716366	4.688240229179936E-6	0.0	9.845304481277865E-5	4.688240229179936E-6
52-53	48.581849524835135	4.688240229179936E-6	0.0	1.0548540515654855E-4	4.688240229179936E-6
54-55	49.46343324989047	4.688240229179936E-6	4.688240229179936E-6	1.383030867608081E-4	4.688240229179936E-6
56-57	50.350926501754685	4.688240229179936E-6	4.688240229179936E-6	1.42991326989988E-4	4.688240229179936E-6
58-59	51.19260861421948	4.688240229179936E-6	4.688240229179936E-6	1.4767956721916796E-4	4.688240229179936E-6
60-61	52.00782092235032	4.688240229179936E-6	4.688240229179936E-6	1.5471192756293787E-4	4.688240229179936E-6
62-63	52.812526884127564	4.688240229179936E-6	4.688240229179936E-6	1.6877664825047767E-4	4.688240229179936E-6
64-65	53.58735469092424	4.688240229179936E-6	4.688240229179936E-6	1.7815312870883755E-4	4.688240229179936E-6
66-67	54.29198782088953	4.688240229179936E-6	4.688240229179936E-6	2.1800317065686698E-4	4.688240229179936E-6
68-69	54.909239185343246	4.688240229179936E-6	4.688240229179936E-6	2.2269141088604693E-4	4.688240229179936E-6
70-71	55.48045203074642	4.688240229179936E-6	4.688240229179936E-6	2.4613261203194664E-4	4.688240229179936E-6
72-73	56.03078814240905	4.688240229179936E-6	4.688240229179936E-6	2.578532126048964E-4	4.688240229179936E-6
74-75	56.55731971430802	4.688240229179936E-6	4.688240229179936E-6	2.6254145283407637E-4	4.688240229179936E-6
76-77	57.06511239001095	4.688240229179936E-6	4.688240229179936E-6	2.8363853386538605E-4	4.688240229179936E-6
78-79	57.54110004399914	4.688240229179936E-6	4.688240229179936E-6	3.3052093615718546E-4	4.688240229179936E-6
80-81	57.9952826926814	4.688240229179936E-6	4.688240229179936E-6	3.7974745856357475E-4	4.688240229179936E-6
82-83	58.44242126041932	4.688240229179936E-6	9.376480458359871E-6	3.9615629936570453E-4	4.688240229179936E-6
84-85	58.88582330069458	4.688240229179936E-6	9.376480458359871E-6	4.0318865970947446E-4	4.688240229179936E-6
86-87	59.32539973694284	4.688240229179936E-6	9.376480458359871E-6	4.0318865970947446E-4	4.688240229179936E-6
88	59.65050575563532	4.688240229179936E-6	9.376480458359871E-6	4.0318865970947446E-4	4.688240229179936E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	3865	0.0	58.79712	1
TGCCCTA	22285	0.0	54.952938	6
GACTTTT	3395	0.0	54.32562	1
TGCTCTA	20110	0.0	48.773163	6
GCCCTAC	59635	0.0	48.530476	7
CCCCTAC	138250	0.0	48.401836	7
GCCCCTA	15810	0.0	47.59849	6
CTCCCTA	45955	0.0	47.531628	6
CACCCTA	24885	0.0	47.41814	6
CACTTTT	5635	0.0	47.091618	1
CCCCCTA	72345	0.0	47.088165	6
ATGCCCT	7020	0.0	46.660744	5
ATTTCTT	16490	0.0	46.27178	2
TCCCCTA	41155	0.0	45.7247	6
CCTTTTC	6815	0.0	45.42746	1
CCACACT	24635	0.0	45.02047	8
GCATTTT	2970	0.0	44.832027	1
ACTTTTC	6000	0.0	44.14846	1
TTGCCCT	9895	0.0	43.979588	5
CCGCCTA	8620	0.0	43.70485	6
>>END_MODULE