##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248487_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21700471
Sequences flagged as poor quality	0
Sequence length	100
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.316314655105874	31.0	30.0	33.0	22.0	34.0
2	29.88246877222158	31.0	30.0	34.0	25.0	34.0
3	30.20551673740169	31.0	30.0	34.0	26.0	34.0
4	33.664077890290955	35.0	35.0	37.0	28.0	37.0
5	33.44151967945765	35.0	33.0	37.0	28.0	37.0
6	33.213675638653186	35.0	33.0	37.0	27.0	37.0
7	33.33015817951601	35.0	33.0	37.0	27.0	37.0
8	33.31793148637188	35.0	33.0	37.0	27.0	37.0
9	34.576373941376666	37.0	34.0	39.0	27.0	39.0
10-11	35.16354776354854	37.0	34.5	39.0	28.5	39.0
12-13	35.4379657473794	38.0	35.0	39.0	30.0	39.0
14-15	36.266831835124684	39.0	35.5	40.0	30.0	41.0
16-17	35.95955080882807	38.0	34.0	40.0	29.0	41.0
18-19	35.4539992933794	38.0	34.0	40.0	27.0	41.0
20-21	34.986618954031	37.0	33.5	39.5	27.0	41.0
22-23	34.615296875353536	37.0	33.0	39.0	27.0	40.5
24-25	34.2729912175639	36.0	33.0	39.0	25.0	40.5
26-27	33.993499449850646	36.0	32.0	39.0	25.0	40.0
28-29	33.83857378026495	35.0	32.0	39.0	25.0	40.0
30-31	33.68969180438526	35.0	32.0	39.0	25.0	40.0
32-33	33.53863095874739	35.0	31.5	39.0	25.0	40.0
34-35	33.33948371443182	35.0	31.0	39.0	24.0	40.0
36-37	33.07736629310949	35.0	30.5	38.5	23.5	40.0
38-39	32.81664653730327	35.0	30.0	38.0	23.0	40.0
40-41	32.533615721981334	35.0	30.0	38.0	22.0	40.0
42-43	32.247674808532956	35.0	29.5	38.0	22.0	40.0
44-45	31.883815056364444	35.0	28.0	38.0	22.0	40.0
46-47	31.740015320404794	34.5	28.0	37.0	21.5	39.0
48-49	31.781517253703846	34.5	28.0	37.0	22.0	39.0
50-51	31.6502019702706	34.0	28.0	37.0	21.5	39.0
52-53	31.549658576535045	34.0	28.0	37.0	21.5	39.0
54-55	31.350182790963387	34.0	28.0	36.0	21.0	39.0
56-57	31.63697610526518	34.0	29.5	36.0	22.5	39.0
58-59	31.778903347305224	34.0	30.0	36.0	23.0	39.0
60-61	31.592230647896997	34.0	30.0	35.0	23.0	38.5
62-63	31.34946916589967	34.0	29.0	35.0	23.0	37.5
64-65	31.14623198731493	34.0	29.0	35.0	22.5	37.0
66-67	30.960496686915228	33.5	29.0	35.0	22.0	36.5
68-69	30.817313988253986	33.0	29.0	35.0	22.0	36.0
70-71	30.704594084616872	33.0	29.0	35.0	22.0	36.0
72-73	30.618097044990407	33.0	29.0	35.0	21.0	35.0
74-75	30.53209522502991	33.0	29.0	35.0	20.5	35.0
76-77	30.39771625233388	33.0	29.0	35.0	20.0	35.0
78-79	30.256919077931535	33.0	29.0	35.0	20.0	35.0
80-81	30.14686460031213	33.0	29.0	35.0	20.0	35.0
82-83	30.01288329179583	33.0	28.5	35.0	19.0	35.0
84-85	29.87589697937893	33.0	28.0	35.0	18.0	35.0
86-87	29.65414017972237	33.0	28.0	35.0	16.0	35.0
88-89	29.405001670240246	33.0	27.5	35.0	15.0	35.0
90-91	29.069378816708635	33.0	27.0	35.0	10.0	35.0
92-93	28.724465911362017	33.0	26.5	35.0	10.0	35.0
94-95	28.42503093596448	33.0	25.5	35.0	9.0	35.0
96-97	28.076992729789136	33.0	25.0	35.0	8.0	35.0
98-99	27.680436797892543	33.0	24.5	35.0	7.5	35.0
100	26.004052124029936	30.0	20.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24638791517024927
1101	2	0.29742980221595516
1101	3	0.37282448397906265
1101	4	0.27369497405189946
1101	5	0.18975762406290642
1101	6	0.12485703423042338
1101	7	0.24373856278712225
1101	8	0.2830268449298643
1101	9	0.3326736545593576
1101	10-11	0.49764214456167366
1101	12-13	0.5841651746688044
1101	14-15	0.7726332795702646
1101	16-17	0.8102973826741149
1101	18-19	0.8061555667205056
1101	20-21	0.7132224554877666
1101	22-23	0.7251348668730131
1101	24-25	0.7370509613649432
1101	26-27	0.7804258978828358
1101	28-29	0.8139021761992993
1101	30-31	0.7664024417773518
1101	32-33	0.7476715034391432
1101	34-35	0.8013013320997047
1101	36-37	0.7905310780273922
1101	38-39	0.755478859970772
1101	40-41	0.7662691605583873
1101	42-43	0.7631594139916729
1101	44-45	0.7312876396618648
1101	46-47	0.7903129248187177
1101	48-49	0.7885006579977549
1101	50-51	0.7314833835175243
1101	52-53	0.6874571361182262
1101	54-55	0.6381261350220342
1101	56-57	0.6207784421245215
1101	58-59	0.5956998176994475
1101	60-61	0.6214892705810477
1101	62-63	0.56582247106412
1101	64-65	0.5876645197970305
1101	66-67	0.5918192662319193
1101	68-69	0.5968573282921064
1101	70-71	0.6120918332937784
1101	72-73	0.619365811096916
1101	74-75	0.5535438550450351
1101	76-77	0.5711756134683341
1101	78-79	0.571115298184008
1101	80-81	0.5562662393260034
1101	82-83	0.5625981428212263
1101	84-85	0.5268617941408067
1101	86-87	0.4988294462152112
1101	88-89	0.4737578809524905
1101	90-91	0.4882322494758746
1101	92-93	0.41394782567644484
1101	94-95	0.39906361121110834
1101	96-97	0.37077207573277704
1101	98-99	0.37268217186122143
1101	100	0.4442061174646845
1102	1	0.1711825128551574
1102	2	0.26446328977496947
1102	3	0.2367938644342118
1102	4	0.1964394844209636
1102	5	0.15386136815722296
1102	6	0.021624409928911348
1102	7	0.14398325261990408
1102	8	0.2179398602518745
1102	9	0.17589749697295787
1102	10-11	0.3012398217104817
1102	12-13	0.3932833163548537
1102	14-15	0.46051346083169875
1102	16-17	0.4954010608523731
1102	18-19	0.4566479299193702
1102	20-21	0.40785535175112386
1102	22-23	0.4671603663017123
1102	24-25	0.40271416904502644
1102	26-27	0.4172921317557865
1102	28-29	0.43262425024752105
1102	30-31	0.37605789037284154
1102	32-33	0.3897435030831389
1102	34-35	0.43779408401859854
1102	36-37	0.4123951087953728
1102	38-39	0.34875701485775323
1102	40-41	0.3472119218572729
1102	42-43	0.42608863153152043
1102	44-45	0.37194318526590564
1102	46-47	0.3903994381135938
1102	48-49	0.3735096766509649
1102	50-51	0.4452253073386139
1102	52-53	0.4021586614742745
1102	54-55	0.41663033926346316
1102	56-57	0.40527938596676094
1102	58-59	0.45374269408466006
1102	60-61	0.4535628537756331
1102	62-63	0.4453006981913852
1102	64-65	0.456877129795874
1102	66-67	0.45873060693372025
1102	68-69	0.46380536320100063
1102	70-71	0.47487963897653884
1102	72-73	0.4586320940151758
1102	74-75	0.411513684792034
1102	76-77	0.4128051735277367
1102	78-79	0.45147841461981386
1102	80-81	0.4901345013396927
1102	82-83	0.5062717699267303
1102	84-85	0.4346866984028921
1102	86-87	0.42930053030564963
1102	88-89	0.4205887125428944
1102	90-91	0.3828605331307706
1102	92-93	0.41112237002149143
1102	94-95	0.4380289782765203
1102	96-97	0.40640609434397845
1102	98-99	0.36282607981072346
1102	100	0.347823541403546
1103	1	0.1878341856605843
1103	2	0.1780240878271222
1103	3	0.22480123675945762
1103	4	0.18494734852919947
1103	5	0.17034770624619
1103	6	0.139191936051553
1103	7	0.13984270020541345
1103	8	0.18544909265728649
1103	9	0.21661481006601946
1103	10-11	0.2749412469946009
1103	12-13	0.3729377532970304
1103	14-15	0.4274692468413477
1103	16-17	0.40466288069194434
1103	18-19	0.41477570045742596
1103	20-21	0.3928284971993463
1103	22-23	0.32527337515236354
1103	24-25	0.3377477062998224
1103	26-27	0.31452704311799096
1103	28-29	0.36074609184415607
1103	30-31	0.3514296472011793
1103	32-33	0.38598481129415774
1103	34-35	0.37678025764947165
1103	36-37	0.3332086058275294
1103	38-39	0.34377371433967596
1103	40-41	0.24732953892750942
1103	42-43	0.3064868149833302
1103	44-45	0.26031993948549825
1103	46-47	0.25305802680957257
1103	48-49	0.3285868728868131
1103	50-51	0.31675183375214644
1103	52-53	0.38484838152264444
1103	54-55	0.3522375757063756
1103	56-57	0.358460909793056
1103	58-59	0.38600203307351677
1103	60-61	0.39697612032056995
1103	62-63	0.34959537942775754
1103	64-65	0.3401918874536385
1103	66-67	0.38502125757167605
1103	68-69	0.42776922297179354
1103	70-71	0.40618603298918643
1103	72-73	0.42415650880461797
1103	74-75	0.39605582707785203
1103	76-77	0.40673539117679525
1103	78-79	0.39451506737108843
1103	80-81	0.4002946387330404
1103	82-83	0.4058596859198964
1103	84-85	0.3675542211221128
1103	86-87	0.4124411937292862
1103	88-89	0.3563723151313667
1103	90-91	0.38329165585617986
1103	92-93	0.3411356205076679
1103	94-95	0.332403147063701
1103	96-97	0.3369136702721427
1103	98-99	0.3385202556889446
1103	100	0.2872290385539813
1104	1	0.14896253105496982
1104	2	0.14129184767619662
1104	3	0.19888941306263774
1104	4	0.17593387080670198
1104	5	0.10701204255565244
1104	6	0.09302314377396925
1104	7	0.1158828609395357
1104	8	0.21035797478164397
1104	9	0.14486361165270978
1104	10-11	0.20098137581175024
1104	12-13	0.25930411400725006
1104	14-15	0.30341064088060676
1104	16-17	0.32703593641576134
1104	18-19	0.315938142830376
1104	20-21	0.2890725825276803
1104	22-23	0.27877946221843786
1104	24-25	0.30195254393596116
1104	26-27	0.26860438533353204
1104	28-29	0.23642386028188866
1104	30-31	0.2431956349789175
1104	32-33	0.320129589350195
1104	34-35	0.29090386527315104
1104	36-37	0.3157701796454617
1104	38-39	0.2615394871720298
1104	40-41	0.24449109520840295
1104	42-43	0.24609158820423715
1104	44-45	0.2473353837973491
1104	46-47	0.3424311234205071
1104	48-49	0.3156789589059379
1104	50-51	0.3156209956573086
1104	52-53	0.33997216040642897
1104	54-55	0.34951509277553683
1104	56-57	0.32586463595291804
1104	58-59	0.3282276293655073
1104	60-61	0.3667644384248412
1104	62-63	0.34656103782354464
1104	64-65	0.3338179226939495
1104	66-67	0.27518039063400224
1104	68-69	0.30837608540494443
1104	70-71	0.3348359500011604
1104	72-73	0.364728969252301
1104	74-75	0.3454800761874779
1104	76-77	0.29641729467239486
1104	78-79	0.3796292302221218
1104	80-81	0.3517754208470727
1104	82-83	0.35363221445766513
1104	84-85	0.3747533580054139
1104	86-87	0.3407977750404143
1104	88-89	0.3840849131833295
1104	90-91	0.3542497831350637
1104	92-93	0.3695205002875461
1104	94-95	0.34497921101557694
1104	96-97	0.35576866733345724
1104	98-99	0.31366176646966437
1104	100	0.30259949827258126
1105	1	0.09871601773450323
1105	2	0.14584070917371506
1105	3	0.15195412108894857
1105	4	0.18882908126871456
1105	5	0.08567128678451041
1105	6	0.11902155728235186
1105	7	0.11746112091144312
1105	8	0.12628742097944468
1105	9	0.1456100883482705
1105	10-11	0.23285479301446088
1105	12-13	0.2754647997510844
1105	14-15	0.3274275800208386
1105	16-17	0.35467121206799135
1105	18-19	0.27693680786353525
1105	20-21	0.23817834000622895
1105	22-23	0.20798482423964515
1105	24-25	0.2054018984778594
1105	26-27	0.19597289719957445
1105	28-29	0.1748382176859664
1105	30-31	0.1814726572828036
1105	32-33	0.1610011430749978
1105	34-35	0.2066657486577057
1105	36-37	0.2706626152267475
1105	38-39	0.23187885494741067
1105	40-41	0.21281547479949126
1105	42-43	0.23971645417801568
1105	44-45	0.23209425104163017
1105	46-47	0.18099873992913373
1105	48-49	0.2589476933116437
1105	50-51	0.29083769156960315
1105	52-53	0.33441264912605817
1105	54-55	0.3537462561780984
1105	56-57	0.3530818774431772
1105	58-59	0.31817288669657984
1105	60-61	0.3371267361223964
1105	62-63	0.33044146790197004
1105	64-65	0.29741772490949714
1105	66-67	0.2932458991127973
1105	68-69	0.3451127380098704
1105	70-71	0.3990842622528703
1105	72-73	0.40122462408918125
1105	74-75	0.3916179019063293
1105	76-77	0.36841942897707014
1105	78-79	0.36927281933528633
1105	80-81	0.297356487555998
1105	82-83	0.3455080814656668
1105	84-85	0.34836639944630576
1105	86-87	0.3500534190095088
1105	88-89	0.4302865874611541
1105	90-91	0.3936703204311023
1105	92-93	0.36519434748514
1105	94-95	0.40457171058823604
1105	96-97	0.320672608932842
1105	98-99	0.2921268640054535
1105	100	0.330517894655582
1106	1	-0.002979073508466712
1106	2	0.04114925255336033
1106	3	0.10030395656259117
1106	4	0.0798098269985914
1106	5	0.04087365038088109
1106	6	-0.01774241539884258
1106	7	0.10697373363867513
1106	8	0.10372693183725801
1106	9	0.0359139866626208
1106	10-11	0.10888877781812312
1106	12-13	0.18515952316258932
1106	14-15	0.19076170349175214
1106	16-17	0.19765955914714795
1106	18-19	0.11099044510446276
1106	20-21	0.12117943564442157
1106	22-23	0.1294634807421673
1106	24-25	0.13633845458077332
1106	26-27	0.09560291950302258
1106	28-29	0.1779644130848368
1106	30-31	0.14779310367152476
1106	32-33	0.13574326708926776
1106	34-35	0.23215766666314153
1106	36-37	0.21185330052171736
1106	38-39	0.2323705169923187
1106	40-41	0.21126853877149898
1106	42-43	0.2159180122300839
1106	44-45	0.22018378963522878
1106	46-47	0.17347913581419405
1106	48-49	0.24227502126087685
1106	50-51	0.2522574258145802
1106	52-53	0.27392882482565994
1106	54-55	0.2583382098568201
1106	56-57	0.24205467445045414
1106	58-59	0.2413508658428114
1106	60-61	0.24472938625655516
1106	62-63	0.25939272396713164
1106	64-65	0.24793555560482972
1106	66-67	0.22605785887942886
1106	68-69	0.23335178167070936
1106	70-71	0.25011198510419774
1106	72-73	0.21795700386978112
1106	74-75	0.19074620736419945
1106	76-77	0.18865693184671173
1106	78-79	0.19963525011607786
1106	80-81	0.20774228708620868
1106	82-83	0.18582075919561092
1106	84-85	0.1610158743971084
1106	86-87	0.20130348463109016
1106	88-89	0.17263727977404386
1106	90-91	0.13540235304873605
1106	92-93	0.09002362592929458
1106	94-95	0.10293279631108021
1106	96-97	0.13445396324508252
1106	98-99	0.1311706076791097
1106	100	0.12007106166447201
1107	1	0.012221287516510415
1107	2	0.059096954293991644
1107	3	0.07698324964620795
1107	4	0.028185388856478255
1107	5	0.06415002436988715
1107	6	-0.0031467950993757654
1107	7	0.07330607816529522
1107	8	0.08348520767563627
1107	9	-0.024747872937034288
1107	10-11	0.12468728566946652
1107	12-13	0.19667796462138654
1107	14-15	0.24518625399522165
1107	16-17	0.2704351804908285
1107	18-19	0.16957437973840683
1107	20-21	0.18666325988536414
1107	22-23	0.1821060995404622
1107	24-25	0.1843393406175693
1107	26-27	0.11565728611965653
1107	28-29	0.2550207921169445
1107	30-31	0.2150459704264449
1107	32-33	0.21172708215929958
1107	34-35	0.289224484999302
1107	36-37	0.31137730690415566
1107	38-39	0.26313261498515317
1107	40-41	0.2897419925434619
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2216	28-29	0.17487349925385587
2216	30-31	0.12935732555169466
2216	32-33	0.14673573074337298
2216	34-35	0.09670307315366244
2216	36-37	0.1768309054191235
2216	38-39	0.1984461163223017
2216	40-41	0.17925829395360182
2216	42-43	0.29000632911164814
2216	44-45	0.2618444188287441
2216	46-47	0.19004933589789985
2216	48-49	0.11490584890000477
2216	50-51	0.08121571419220786
2216	52-53	0.12564086240332273
2216	54-55	0.13439710685528894
2216	56-57	0.10904960582901069
2216	58-59	0.025405299867113484
2216	60-61	0.027652814017006477
2216	62-63	0.015925846073340466
2216	64-65	-0.005540971947183948
2216	66-67	-0.030798479367220466
2216	68-69	-0.04811930868732972
2216	70-71	-0.08971398718654555
2216	72-73	-0.15974349378435804
2216	74-75	-0.1703711423835017
2216	76-77	-0.24818433931922712
2216	78-79	-0.23538431907245538
2216	80-81	-0.2776856526650029
2216	82-83	-0.321161758291435
2216	84-85	-0.37311146022888053
2216	86-87	-0.3914538973423092
2216	88-89	-0.41986485079674907
2216	90-91	-0.4327460514422192
2216	92-93	-0.308810568354577
2216	94-95	-0.31146924293684464
2216	96-97	-0.3767816179882182
2216	98-99	-0.27464161260990494
2216	100	0.058263219375948694
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	615.0
6	4490.0
7	13858.0
8	28595.0
9	49043.0
10	68857.0
11	81628.0
12	85220.0
13	87712.0
14	93471.0
15	102645.0
16	113073.0
17	126516.0
18	143303.0
19	160708.0
20	181524.0
21	205090.0
22	229296.0
23	265289.0
24	373409.0
25	477070.0
26	405118.0
27	737236.0
28	567442.0
29	882854.0
30	880913.0
31	1080505.0
32	1387608.0
33	2170051.0
34	2644834.0
35	5011741.0
36	2252602.0
37	689589.0
38	95678.0
39	2877.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.397611849003	30.108795788368152	26.028086118004722	27.465506244624123
2	14.202753906199597	31.483560469182652	28.24710963533535	26.0665759892824
3	12.906199132728503	33.74558552208383	25.437549258723465	27.910666086464207
4	11.688341851044786	32.80477947273858	25.652998559616385	29.853880116600244
5	11.6727471801302	32.71052654619923	26.36771922451449	29.249007049156077
6	11.419358118083043	29.3185087085001	27.902717005579024	31.35941616783783
7	15.905925069413454	33.767426634084785	23.162863695993863	27.163784600507903
8	13.826847352760224	31.299191616624356	20.932471926530997	33.94148910408442
9	10.833986045740666	29.96099024763103	22.347809870117565	36.85721383651074
10-11	16.4624066022379	43.656516553992475	20.76349792546446	19.117578918305167
12-13	16.087488257452897	45.03822302621928	19.721965424107356	19.15232329222047
14-15	17.44883119322478	43.96141652641182	19.152492682270665	19.437259598092734
16-17	17.556625263030515	43.72607794988753	19.713866025413598	19.00343076166835
18-19	18.22472642247181	41.971486279304145	20.83364016289078	18.970147135333264
20-21	17.176329187022326	41.65107233122698	21.80669764313001	19.365900838620682
22-23	17.470681955191914	42.573841068239204	22.42297604962992	17.532500926938962
24-25	17.479943177270208	42.5476963149786	21.787745989476452	18.184614518274742
26-27	19.28583024764762	42.78756207641761	20.430853781929432	17.495753894005343
28-29	18.636715304474265	43.759888898264	21.2302097037433	16.37318609351843
30-31	18.94598096392867	43.385656328017596	21.14726648202673	16.521096226027005
32-33	19.527536765232774	43.52869508163905	20.46043399723167	16.483334155896504
34-35	19.620669069228764	43.51587720896968	20.36669063698924	16.496763084812315
36-37	20.19454032192992	43.196816727699996	20.103565195484315	16.505077754885768
38-39	20.62918936196009	43.347616819654206	20.048986765237114	15.974207053148588
40-41	20.982081211194448	43.5963277495571	19.976387665872224	15.445203373376238
42-43	21.247999936590592	43.94626891271457	20.093280494309525	14.71245065638531
44-45	21.475750035874977	43.79125564817767	19.943139658223213	14.789854657724144
46-47	21.502554374833053	43.40315358029637	20.2393771206917	14.85491492417888
48-49	21.313914530758982	43.51461881330849	20.65292873841323	14.518537917519298
50-51	21.47507535665931	43.223398238683394	21.070644042703034	14.230882361954263
52-53	21.5430978844696	43.19001161473904	21.38221869141897	13.884671809372385
54-55	21.517921939723475	43.27377528997602	21.444084494129513	13.764218276170986
56-57	21.296045361813466	43.42828856304596	21.06522668487588	14.210439390264689
58-59	21.049315116027252	43.275106404367726	21.136638574268453	14.538939905336571
60-61	21.037968295649762	42.91916293665749	21.43873628856862	14.604132479124118
62-63	21.206423515486854	42.24649312703474	21.67046978002387	14.87661357745454
64-65	21.101916798290393	42.024348686338335	21.7161926019766	15.15754191339467
66-67	20.68527682231145	41.784188352971384	22.123048036726463	15.407486787990699
68-69	20.378458102729404	41.89464622549019	21.83830591139436	15.888589760386044
70-71	19.813173412625495	42.11639795924833	22.01292721829787	16.057501409828305
72-73	19.627612620887685	42.703449221659206	22.004983373662544	15.663954783790562
74-75	19.28989052529238	43.392067809992724	21.856933009139624	15.461108655575273
76-77	18.61937043527789	44.35904147344817	21.892396864756808	15.129191226517127
78-79	18.106191444928545	45.32154120648482	21.58377963288799	14.98848771569865
80-81	17.532032517500316	46.635810037887346	21.034924776339594	14.797232668272747
82-83	17.023870431776952	47.73280378813599	20.77040807921243	14.472917700874627
84-85	16.472485604764632	48.91919232997574	20.51523197408702	14.093090091172607
86-87	16.03857670314537	49.76173832166627	20.170108605703575	14.029576369484781
88-89	15.73668983417586	50.31785625379431	20.022520050259406	13.92293386177042
90-91	15.626559964203125	50.70702632358408	19.728523458347823	13.937890253864968
92-93	15.687734097930353	50.825036220664025	19.381221106416863	14.106008574988756
94-95	15.79801248566755	50.88994418815408	19.012068553915203	14.299974772263166
96-97	15.976896577331562	50.56603159734704	18.837751095495786	14.619320729825617
98-99	16.23270046568489	50.03295215224915	18.665256616080896	15.069090765985067
100	16.7857842157101	48.88033897085074	18.44273572042158	15.891141093017586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1569890.0
1	1552548.5
2	979184.5
3	265318.5
4	106253.0
5	101785.0
6	87155.0
7	62830.0
8	41455.5
9	30676.5
10	29090.5
11	33019.0
12	40982.5
13	52288.5
14	68034.5
15	88010.5
16	110821.0
17	135688.5
18	162304.0
19	189119.5
20	213259.0
21	234745.0
22	253548.0
23	268549.5
24	278803.5
25	286033.0
26	290434.5
27	288899.0
28	283056.5
29	275070.0
30	264057.0
31	251390.0
32	238570.5
33	226367.5
34	215165.5
35	204461.5
36	194988.5
37	186761.0
38	181287.5
39	180668.5
40	186257.5
41	201023.0
42	228298.0
43	273577.0
44	340100.0
45	422890.0
46	513340.0
47	599940.5
48	673660.0
49	735567.5
50	794034.0
51	854649.0
52	912447.5
53	946534.0
54	931855.0
55	854516.5
56	716009.5
57	546060.5
58	381587.5
59	246149.5
60	150330.5
61	90064.0
62	56281.5
63	38456.0
64	28642.5
65	22910.5
66	19188.0
67	16424.0
68	14136.5
69	12473.5
70	11118.0
71	9920.5
72	9001.0
73	8168.5
74	7483.5
75	6991.0
76	6579.0
77	6207.5
78	5832.5
79	5576.5
80	5271.0
81	5012.0
82	4822.0
83	4621.5
84	4481.0
85	4401.0
86	4409.5
87	4289.5
88	4119.5
89	3967.5
90	3824.5
91	3676.5
92	3571.0
93	3518.5
94	3361.0
95	3139.5
96	2944.5
97	2693.0
98	2389.5
99	2000.0
100	1464.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013124139102787214
2	4.377785164202197E-4
3	0.0
4	9.216389819373046E-6
5	4.608194909686523E-6
6	0.003732637876846083
7	3.0874905894899703E-4
8	0.0
9	0.0
10-11	3.2948593604258634E-4
12-13	2.073687709358935E-5
14-15	4.2395393169116E-4
16-17	1.9354418620683397E-4
18-19	1.8893599129714744E-4
20-21	7.603521600982762E-5
22-23	5.9906533825924795E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	1.728073091132446E-4
32-33	4.608194909686522E-5
34-35	1.1981306765184959E-4
36-37	1.5667862692934177E-4
38-39	5.529833891623827E-4
40-41	0.001711944408948543
42-43	0.0010207151724955647
44-45	5.02293245155831E-4
46-47	6.981415288175082E-4
48-49	0.0010667971215924299
50-51	0.0
52-53	1.2211716510669287E-4
54-55	4.58515393513809E-4
56-57	4.7925227060739836E-4
58-59	5.368547069784799E-4
60-61	4.838604655170849E-5
62-63	2.2349745311979631E-4
64-65	1.8202369893261764E-4
66-67	2.0045647857136375E-4
68-69	1.1059667783247655E-4
70-71	6.151940204431508E-4
72-73	5.138137324300473E-4
74-75	3.2487774113289984E-4
76-77	3.7326378768460833E-4
78-79	7.972177193757684E-4
80-81	0.0010022823928568186
82-83	4.0782524950725725E-4
84-85	6.843169440884486E-4
86-87	0.001269557697618637
88-89	0.0011658733121506903
90-91	0.0011681774096055334
92-93	6.958374313626649E-4
94-95	6.889251389981351E-4
96-97	6.290186051722103E-4
98-99	3.110531564038403E-4
100	9.124225921179315E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.1700471E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.18336499684227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.26640829983042	74.48248372043845
2	4.173558988439518	6.526057714580373
3	0.34188726488830645	0.8018969045560369
4	0.06482061958666642	0.20271576641863212
5	0.03011621009990197	0.11772933232811116
6	0.025890044786769536	0.12145024928091577
7	0.019295811576405694	0.10560280355718947
8	0.010376557502342698	0.0649019346093105
9	0.010344161513339835	0.0727867219665363
>10	0.049561646682451124	0.7317712203728243
>50	0.003435788635299481	0.18502067860884952
>100	0.002754494136903563	0.43313318514313914
>500	4.173556559719428E-4	0.23690366033762264
>1k	6.373480714142812E-4	1.0690939274454219
>5k	1.533407553501473E-4	0.884641284839621
>10k+	3.4206783885801624E-4	13.963810895516964
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	741217	3.415672406373115	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	190916	0.8797781393777121	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	174907	0.8060055470685406	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	151261	0.697040170234093	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	120267	0.554213777203269	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89302	0.4115210218248258	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	84991	0.3916550935691672	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	84262	0.38829571948000574	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	80826	0.37246196177032287	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69184	0.31881335663175236	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66540	0.3066292892905412	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63915	0.2945327776526141	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61711	0.284376316071665	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56414	0.25996670763505547	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51162	0.23576446796938186	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44712	0.2060416108019038	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44356	0.20440109341405538	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44276	0.2040324378212805	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43467	0.20030440813934408	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40822	0.18811573260322323	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40277	0.18560426637744407	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38454	0.17720352705708553	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34362	0.1583467934866483	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30534	0.14070662337236828	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30433	0.14024119568648996	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28784	0.13264228228041686	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28443	0.13107088781621376	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27811	0.12815850863329187	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26260	0.12101119832836808	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002548331785056647	0.0	0.0	4.608194909686522E-6	0.0
2	0.006147332009521821	0.0	0.0	1.843277963874609E-5	0.0
3	0.010437561470439973	0.0	0.0	2.7649169458119136E-5	0.0
4	0.016976590047285148	0.0	0.0	3.2257364367805655E-5	0.0
5	0.028192936457462143	0.0	0.0	3.686555927749218E-5	0.0
6	0.048598023517554066	0.0	0.0	3.686555927749218E-5	0.0
7	0.0735467907585969	0.0	0.0	3.686555927749218E-5	0.0
8	0.16079374498369184	0.0	0.0	3.686555927749218E-5	0.0
9	0.4827176331794826	0.0	0.0	4.14737541871787E-5	0.0
10-11	1.2066719657835998	0.0	0.0	4.377785164202196E-5	0.0
12-13	2.1810217851953535	0.0	0.0	4.608194909686522E-5	0.0
14-15	3.2973316569949107	0.0	0.0	4.608194909686522E-5	0.0
16-17	4.522051618142298	0.0	0.0	6.681882619045457E-5	0.0
18-19	5.878280245622319	0.0	0.0	7.833931346467088E-5	0.0
20-21	7.639262760702291	0.0	0.0	8.755570328404391E-5	0.0
22-23	9.614818959459452	0.0	0.0	9.216389819373044E-5	0.0
24-25	11.608075234864717	0.0	0.0	9.216389819373044E-5	0.0
26-27	13.535837540116065	0.0	2.304097454843261E-6	9.216389819373044E-5	0.0
28-29	15.478122571625288	0.0	4.608194909686522E-6	1.1290077528731981E-4	0.0
30-31	17.465763761533104	0.0	9.216389819373044E-6	1.1520487274216306E-4	0.0
32-33	19.67659365550176	0.0	9.216389819373044E-6	1.1981306765184959E-4	0.0
34-35	21.739804633733527	0.0	9.216389819373044E-6	1.3363765238090915E-4	0.0
36-37	23.468451445132228	0.0	9.216389819373044E-6	1.4054994474543893E-4	0.0
38-39	24.843631274178335	0.0	9.216389819373044E-6	1.7511140656808786E-4	0.0
40-41	26.05485844063016	0.0	9.216389819373044E-6	2.4884252512307224E-4	0.0
42-43	27.07129029595717	0.0	9.216389819373044E-6	2.857080844005644E-4	0.0
44-45	27.924557490019456	0.0	9.216389819373044E-6	3.2026954622321334E-4	0.0
46-47	28.673015899055834	0.0	9.216389819373044E-6	3.502228131361757E-4	0.0
48-49	29.37442233396685	0.0	9.216389819373044E-6	4.032170545975707E-4	0.0
50-51	30.083598185495603	2.304097454843261E-6	9.216389819373044E-6	4.4929900369443593E-4	0.0
52-53	30.828300915680586	9.216389819373044E-6	9.216389819373044E-6	5.069014400655175E-4	0.0
54-55	31.600844055412438	9.216389819373044E-6	1.3824584729059568E-5	5.944571433495614E-4	0.0
56-57	32.41352457280766	9.216389819373044E-6	1.3824584729059568E-5	6.290186051722104E-4	0.0
58-59	33.24490284104893	9.216389819373044E-6	1.3824584729059568E-5	6.451472873561132E-4	0.0
60-61	34.09029232591311	9.216389819373044E-6	1.3824584729059568E-5	6.727964568142323E-4	0.0
62-63	34.90035078040472	9.216389819373044E-6	1.3824584729059568E-5	7.050538211820379E-4	0.0
64-65	35.6639425015245	9.216389819373044E-6	1.3824584729059568E-5	7.764808422821791E-4	0.0
66-67	36.36057254241163	9.216389819373044E-6	1.3824584729059568E-5	8.663406430210662E-4	0.0
68-69	36.98487235599633	9.216389819373044E-6	1.3824584729059568E-5	9.124225921179315E-4	0.0
70-71	37.55744748581724	1.1520487274216305E-5	1.3824584729059568E-5	9.400717615760505E-4	0.0
72-73	38.09572151682791	1.3824584729059568E-5	1.3824584729059568E-5	9.769373208535427E-4	0.0
74-75	38.607931597429385	1.3824584729059568E-5	1.3824584729059568E-5	0.0010621889266827435	0.0
76-77	39.104863207807796	1.3824584729059568E-5	1.3824584729059568E-5	0.0011658733121506903	0.0
78-79	39.58901629370165	1.843277963874609E-5	1.3824584729059568E-5	0.0013087273543509724	0.0
80-81	40.05933788257407	1.843277963874609E-5	1.3824584729059568E-5	0.0014976633456481197	0.0
82-83	40.52625862360315	1.843277963874609E-5	1.3824584729059568E-5	0.001587523146387007	0.0
84-85	40.99403418478797	1.843277963874609E-5	1.3824584729059568E-5	0.0016128682183902828	0.0
86-87	41.463010180746764	1.843277963874609E-5	1.3824584729059568E-5	0.001624388705664499	0.0
88	41.81882503840585	1.843277963874609E-5	1.3824584729059568E-5	0.0016451255827580884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	10805	0.0	46.28181	9
GCCTGCT	2445	0.0	43.058968	7
CCTGCTG	7400	0.0	40.902195	8
GCTTGCT	2700	0.0	40.210793	7
CTTGCTG	7285	0.0	37.547916	8
CTGCTGA	9630	0.0	36.750404	9
TACCCGA	17040	0.0	30.50661	6
ACCCGAC	18620	0.0	28.85101	7
TTTGCTG	4090	0.0	28.84316	8
ATTGCTG	2025	0.0	28.77989	8
TGACGAC	1425	0.0	28.695438	6
ACTGGCC	1215	0.0	28.626017	3
TGCCAAA	2350	0.0	28.400694	6
TTGCGCC	1460	0.0	28.329264	3
TGACGCC	1690	0.0	27.533026	3
CCCGACG	21235	0.0	27.444912	8
TCGCGAC	1460	0.0	27.363682	6
GCTGAAC	21035	0.0	27.18085	10-11
TGAAAAA	15755	0.0	26.94194	1
TTCGCGA	1225	0.0	26.857798	5
>>END_MODULE