##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248486_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22371353
Sequences flagged as poor quality	0
Sequence length	100
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.2804390507807	31.0	30.0	33.0	20.0	34.0
2	29.871374207898825	31.0	30.0	34.0	24.0	34.0
3	30.24635018722381	31.0	30.0	34.0	25.0	34.0
4	33.741056698716434	35.0	35.0	37.0	28.0	37.0
5	33.54788988399584	35.0	35.0	37.0	28.0	37.0
6	33.30641007720901	35.0	33.0	37.0	27.0	37.0
7	33.38583012837891	35.0	33.0	37.0	28.0	37.0
8	33.3553678224111	35.0	33.0	37.0	27.0	37.0
9	34.625032647779506	37.0	34.0	39.0	27.0	39.0
10-11	35.29163902156477	37.0	34.5	39.0	29.5	39.0
12-13	35.590866006182104	39.0	35.0	39.0	30.0	39.0
14-15	36.462565183250206	39.0	36.0	40.0	30.0	41.0
16-17	36.162543432218875	39.0	35.0	40.0	30.0	41.0
18-19	35.60785340073084	38.0	34.0	40.0	28.5	41.0
20-21	35.10573312217638	37.0	33.5	39.5	27.0	41.0
22-23	34.696443415827375	37.0	33.0	39.0	27.0	40.5
24-25	34.335726185179766	36.0	33.0	39.0	25.5	40.0
26-27	34.05362523223339	35.0	33.0	39.0	25.0	40.0
28-29	33.89561395772531	35.0	33.0	39.0	25.0	40.0
30-31	33.742656490199764	35.0	32.5	39.0	25.0	40.0
32-33	33.603581374805536	35.0	32.0	39.0	25.0	40.0
34-35	33.42191250122423	35.0	31.0	39.0	25.0	40.0
36-37	33.15413283228779	35.0	30.5	38.0	24.0	40.0
38-39	32.89512384432001	35.0	30.0	38.0	23.5	40.0
40-41	32.618595687976494	35.0	30.0	38.0	23.0	40.0
42-43	32.365208130236915	35.0	30.0	38.0	22.5	40.0
44-45	32.01241777821842	35.0	28.5	37.5	22.0	40.0
46-47	31.89203156375924	35.0	28.5	37.0	22.0	39.0
48-49	31.94603223148819	35.0	28.5	37.0	22.0	39.0
50-51	31.830388644799445	34.0	28.5	37.0	22.0	39.0
52-53	31.742788668168618	34.0	28.0	37.0	22.0	39.0
54-55	31.577285915608233	34.0	28.5	36.0	22.0	39.0
56-57	31.873759423491283	34.0	29.5	36.0	23.0	39.0
58-59	32.01448367919455	34.0	30.0	36.0	24.0	39.0
60-61	31.844863227539257	34.0	30.0	35.5	24.0	39.0
62-63	31.61417496742374	34.0	30.0	35.0	23.0	38.0
64-65	31.41866394938205	34.0	29.0	35.0	23.0	37.5
66-67	31.23981674242054	34.0	29.0	35.0	23.0	37.0
68-69	31.09998838693395	34.0	29.0	35.0	23.0	36.5
70-71	30.985508587701425	34.0	29.0	35.0	23.0	36.0
72-73	30.8940328285017	33.5	29.0	35.0	23.0	36.0
74-75	30.801136100261793	33.0	29.0	35.0	23.0	35.0
76-77	30.662272617127805	33.0	29.0	35.0	21.5	35.0
78-79	30.514766205691718	33.0	29.0	35.0	20.0	35.0
80-81	30.396411875490948	33.0	29.0	35.0	20.0	35.0
82-83	30.251417828863545	33.0	29.0	35.0	20.0	35.0
84-85	30.099444432350605	33.0	29.0	35.0	19.0	35.0
86-87	29.868978219600756	33.0	28.0	35.0	17.5	35.0
88-89	29.61975227872896	33.0	28.0	35.0	15.5	35.0
90-91	29.28942952176384	33.0	27.5	35.0	11.0	35.0
92-93	28.948615199983657	33.0	27.0	35.0	10.0	35.0
94-95	28.64643012427545	33.0	26.5	35.0	10.0	35.0
96-97	28.286432206402537	33.0	25.0	35.0	8.0	35.0
98-99	27.872591724783028	33.0	25.0	35.0	8.0	35.0
100	26.262613664895458	31.0	20.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3183952509174439
1101	2	0.3315830009152556
1101	3	0.3588740659326177
1101	4	0.2502719278073542
1101	5	0.17109912087622803
1101	6	0.16244225192426143
1101	7	0.26719835709781137
1101	8	0.26538906581674837
1101	9	0.29114136306438354
1101	10-11	0.461140739897445
1101	12-13	0.5477416370930897
1101	14-15	0.7038411510117726
1101	16-17	0.7568821375560262
1101	18-19	0.703575218168325
1101	20-21	0.6391739722153176
1101	22-23	0.6437816521910449
1101	24-25	0.7019164876305979
1101	26-27	0.6954940969550876
1101	28-29	0.7289207319388638
1101	30-31	0.6820751020668396
1101	32-33	0.7010803747074519
1101	34-35	0.7443935488067339
1101	36-37	0.7440698258863989
1101	38-39	0.7162892365817513
1101	40-41	0.7197801105414712
1101	42-43	0.7372847885024498
1101	44-45	0.6743659947610041
1101	46-47	0.7170516479632809
1101	48-49	0.7290849390273024
1101	50-51	0.6940933127319155
1101	52-53	0.6759582839037854
1101	54-55	0.6133040425913379
1101	56-57	0.614868033473936
1101	58-59	0.5898064141159907
1101	60-61	0.6033776690824197
1101	62-63	0.5715821788710045
1101	64-65	0.5915228790623743
1101	66-67	0.5756945826545277
1101	68-69	0.5794586938003228
1101	70-71	0.5758603106761129
1101	72-73	0.5757987845074908
1101	74-75	0.5361111472759923
1101	76-77	0.565490808541476
1101	78-79	0.5681508853147079
1101	80-81	0.5748577470270426
1101	82-83	0.5454913467494755
1101	84-85	0.5300610078338224
1101	86-87	0.4781766664290963
1101	88-89	0.46298564236753137
1101	90-91	0.4491920543169172
1101	92-93	0.41770778394205266
1101	94-95	0.3854948718555171
1101	96-97	0.36899263121019743
1101	98-99	0.3218525937673782
1101	100	0.3249639771499382
1102	1	0.2353730827521865
1102	2	0.31709104681321776
1102	3	0.3122484696492265
1102	4	0.2637785098985148
1102	5	0.16778754702009024
1102	6	0.07983171642828069
1102	7	0.14070106771939095
1102	8	0.15490790677108635
1102	9	0.17337938077709936
1102	10-11	0.30576394007910324
1102	12-13	0.4153614406975308
1102	14-15	0.4822169035175605
1102	16-17	0.48321807617692514
1102	18-19	0.4537626553977461
1102	20-21	0.4008667367173189
1102	22-23	0.42875360626937464
1102	24-25	0.38114955128514083
1102	26-27	0.3786583029955324
1102	28-29	0.43015628951940954
1102	30-31	0.39802394857048284
1102	32-33	0.3680136964994958
1102	34-35	0.41616413251525586
1102	36-37	0.3962741549957016
1102	38-39	0.35887380121632617
1102	40-41	0.3425023242987706
1102	42-43	0.38081388351530165
1102	44-45	0.30446764158812556
1102	46-47	0.38154613660321246
1102	48-49	0.397419897330451
1102	50-51	0.430643809619923
1102	52-53	0.43676123292014424
1102	54-55	0.38659216974865984
1102	56-57	0.3965286804895847
1102	58-59	0.4523616774174286
1102	60-61	0.41610624438160926
1102	62-63	0.42825713639155083
1102	64-65	0.43571619345534174
1102	66-67	0.46438772748543755
1102	68-69	0.451976165807757
1102	70-71	0.49634144452461726
1102	72-73	0.45316868860412995
1102	74-75	0.42277261542263034
1102	76-77	0.4068600548440173
1102	78-79	0.4016510510230944
1102	80-81	0.4413482731911813
1102	82-83	0.4401040160002836
1102	84-85	0.4148419674365478
1102	86-87	0.4210725716237427
1102	88-89	0.4161396746839259
1102	90-91	0.4394783488995877
1102	92-93	0.44118949198936974
1102	94-95	0.43089905061188105
1102	96-97	0.4123801478255018
1102	98-99	0.4404968694468394
1102	100	0.3898194521668046
1103	1	0.26696235605607654
1103	2	0.27855846130574946
1103	3	0.2297539516176741
1103	4	0.23583733374209004
1103	5	0.1889053398477003
1103	6	0.15158187645877064
1103	7	0.19243218815845609
1103	8	0.1778434556904287
1103	9	0.13480387906835745
1103	10-11	0.20595353648180037
1103	12-13	0.3089796297026268
1103	14-15	0.3774154620339445
1103	16-17	0.3927705370404908
1103	18-19	0.3619280752749603
1103	20-21	0.33610823717744154
1103	22-23	0.29253579641115124
1103	24-25	0.3128021243302683
1103	26-27	0.27595930995501305
1103	28-29	0.3256977786912998
1103	30-31	0.2741386893680797
1103	32-33	0.31794535187750483
1103	34-35	0.3227716381014787
1103	36-37	0.343187735301953
1103	38-39	0.23591144809680742
1103	40-41	0.2185708472380128
1103	42-43	0.2747543360416387
1103	44-45	0.2170447637471682
1103	46-47	0.18743195079590436
1103	48-49	0.2586733252430662
1103	50-51	0.2976209217767902
1103	52-53	0.301939670982339
1103	54-55	0.24285100977259333
1103	56-57	0.2552197720270293
1103	58-59	0.273324207444233
1103	60-61	0.2753781856286466
1103	62-63	0.2703136084854094
1103	64-65	0.2813992720173921
1103	66-67	0.29359084483079556
1103	68-69	0.310073351082611
1103	70-71	0.3074671150782464
1103	72-73	0.2767291890827934
1103	74-75	0.28417438664896366
1103	76-77	0.2986223691597196
1103	78-79	0.332985286538765
1103	80-81	0.3333855014629883
1103	82-83	0.3502112182741328
1103	84-85	0.3231763208548095
1103	86-87	0.36037297719241934
1103	88-89	0.3603506211543994
1103	90-91	0.3241387085787544
1103	92-93	0.33069384351145104
1103	94-95	0.3265916693713429
1103	96-97	0.3086395492700049
1103	98-99	0.2833184960291888
1103	100	0.22489311233919196
1104	1	0.11949092068078926
1104	2	0.14739001494753623
1104	3	0.15536778805694595
1104	4	0.14654809836823546
1104	5	0.1008785326357895
1104	6	0.11725244254657241
1104	7	0.12423928062302281
1104	8	0.11790594524935472
1104	9	0.05863180759168074
1104	10-11	0.12579175752759397
1104	12-13	0.1979481423944165
1104	14-15	0.25918922495346663
1104	16-17	0.2352396556136256
1104	18-19	0.26944561390573796
1104	20-21	0.21928469861472877
1104	22-23	0.22782131798809502
1104	24-25	0.2541854647627133
1104	26-27	0.2761015309604318
1104	28-29	0.20413410766927598
1104	30-31	0.18898970222453215
1104	32-33	0.24940018575491507
1104	34-35	0.2539747424122325
1104	36-37	0.2501249637416052
1104	38-39	0.202706470430563
1104	40-41	0.15562439123984717
1104	42-43	0.20311025102722624
1104	44-45	0.20917531502513498
1104	46-47	0.23723689758018907
1104	48-49	0.24675583201937457
1104	50-51	0.228744803503524
1104	52-53	0.27159473172644155
1104	54-55	0.29567689734888347
1104	56-57	0.2612948857244213
1104	58-59	0.23971445486481002
1104	60-61	0.28118978118242666
1104	62-63	0.28917014551298337
1104	64-65	0.2840793173862153
1104	66-67	0.24009487383403183
1104	68-69	0.3096203578250645
1104	70-71	0.2755663634080747
1104	72-73	0.2963272315326293
1104	74-75	0.29763056134947163
1104	76-77	0.2284502725594848
1104	78-79	0.26864795134646613
1104	80-81	0.2662277038692906
1104	82-83	0.2628037080350616
1104	84-85	0.26663492816154033
1104	86-87	0.2841078083173798
1104	88-89	0.27954577351251686
1104	90-91	0.24739819159852416
1104	92-93	0.25663440266966475
1104	94-95	0.22032864480961933
1104	96-97	0.1995435378259387
1104	98-99	0.2110301779858581
1104	100	0.2131752728904246
1105	1	0.10787586547355232
1105	2	0.11645247277191473
1105	3	0.14681191987932962
1105	4	0.11155707119095837
1105	5	0.05234108492216194
1105	6	0.06072611381762272
1105	7	0.10402028513139783
1105	8	0.08369498667539688
1105	9	0.040027689550981904
1105	10-11	0.14677192393492788
1105	12-13	0.1809247059205248
1105	14-15	0.2511624834050963
1105	16-17	0.2789803442540375
1105	18-19	0.23010079023855212
1105	20-21	0.2154725591800215
1105	22-23	0.1708433745781761
1105	24-25	0.15134220925433084
1105	26-27	0.1497528823014278
1105	28-29	0.12953336676283556
1105	30-31	0.12912716123692292
1105	32-33	0.0957654249585218
1105	34-35	0.18022830356441233
1105	36-37	0.20951291990033383
1105	38-39	0.1998282348443965
1105	40-41	0.1480282126435739
1105	42-43	0.11610499639591865
1105	44-45	0.0986112421331029
1105	46-47	0.08964670570465927
1105	48-49	0.14988005988409725
1105	50-51	0.17343160867819307
1105	52-53	0.1846437914180079
1105	54-55	0.19535621148574478
1105	56-57	0.2587572727647789
1105	58-59	0.20604912893199412
1105	60-61	0.2165028872589545
1105	62-63	0.2567514767091197
1105	64-65	0.23450388720718962
1105	66-67	0.207550913706271
1105	68-69	0.23283953983385786
1105	70-71	0.2666488151258406
1105	72-73	0.2572079104972431
1105	74-75	0.2518796280754003
1105	76-77	0.2395137092288877
1105	78-79	0.30786345971453954
1105	80-81	0.2544666606236454
1105	82-83	0.28549056694386366
1105	84-85	0.2649027196615279
1105	86-87	0.24891660717808506
1105	88-89	0.25878177683579295
1105	90-91	0.22788186678815592
1105	92-93	0.20952247416590453
1105	94-95	0.1977346209588866
1105	96-97	0.16968995367342643
1105	98-99	0.1633754967212937
1105	100	0.1802705592407392
1106	1	-0.05323021245057191
1106	2	-0.03892897726217015
1106	3	0.03169664751889201
1106	4	0.008543846110136144
1106	5	-0.04550358953488853
1106	6	-0.03485134459184991
1106	7	0.026616016556786803
1106	8	0.02473999230244317
1106	9	-0.01827277116492354
1106	10-11	0.051758902837178766
1106	12-13	0.09898100752307215
1106	14-15	0.09814285088154406
1106	16-17	0.09268990272012445
1106	18-19	0.049196153300684387
1106	20-21	0.0509718323703936
1106	22-23	0.0102400749913798
1106	24-25	0.042783827619238934
1106	26-27	0.03733485792844249
1106	28-29	0.06912262696682347
1106	30-31	0.08283449939027321
1106	32-33	0.06421099876219216
1106	34-35	0.11283598541393047
1106	36-37	0.14793461987999024
1106	38-39	0.1864304022376828
1106	40-41	0.15015577327915253
1106	42-43	0.16269988144145486
1106	44-45	0.17064062948541903
1106	46-47	0.15227793891730812
1106	48-49	0.15584071730591376
1106	50-51	0.18508873388555713
1106	52-53	0.18568116487946895
1106	54-55	0.14089223297937892
1106	56-57	0.1727745850635536
1106	58-59	0.21072521506812336
1106	60-61	0.19784075837931425
1106	62-63	0.20695168822201282
1106	64-65	0.2025070278670711
1106	66-67	0.1826940216400601
1106	68-69	0.19014882282057144
1106	70-71	0.18529448814949134
1106	72-73	0.12285507648240568
1106	74-75	0.12149134471733092
1106	76-77	0.13596742970115372
1106	78-79	0.14296460278021783
1106	80-81	0.14408340412472498
1106	82-83	0.12596532920738213
1106	84-85	0.11139039714028698
1106	86-87	0.14038752105357588
1106	88-89	0.14219558287337009
1106	90-91	0.13833746792545298
1106	92-93	0.11661427785515244
1106	94-95	0.14137110620431415
1106	96-97	0.12850125739093698
1106	98-99	0.12824156024397482
1106	100	0.18562987657318786
1107	1	0.04358806595599418
1107	2	0.06755941744179594
1107	3	0.12633557050179078
1107	4	0.08036585223305082
1107	5	0.07396665600913366
1107	6	0.0022294084357952215
1107	7	0.058846341026949744
1107	8	0.05076098181351796
1107	9	-0.05301497275203104
1107	10-11	0.11342590938308206
1107	12-13	0.17556239102165705
1107	14-15	0.2410298106539983
1107	16-17	0.2273912255642898
1107	18-19	0.1322425248225798
1107	20-21	0.1673087028682403
1107	22-23	0.12708103931445436
1107	24-25	0.19312315899977506
1107	26-27	0.19659820337259504
1107	28-29	0.24161382554532906
1107	30-31	0.19114815277315245
1107	32-33	0.21251017665358063
1107	34-35	0.28164460796922697
1107	36-37	0.2606572165245922
1107	38-39	0.2027881246740506
1107	40-41	0.22508040914820526
1107	42-43	0.32086161727201556
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2216	12-13	-0.1692502103926543
2216	14-15	-0.14830095018885459
2216	16-17	-0.11478085060606702
2216	18-19	-0.0589048960784595
2216	20-21	-0.04566596105581766
2216	22-23	0.005276706458495539
2216	24-25	0.11873471212999931
2216	26-27	0.12996470534321247
2216	28-29	0.1532232416171908
2216	30-31	0.14013167892664313
2216	32-33	0.126996011920113
2216	34-35	0.10783910499377214
2216	36-37	0.21362106273871007
2216	38-39	0.2464527842550126
2216	40-41	0.20837540603456972
2216	42-43	0.27907411743891686
2216	44-45	0.2990123895087464
2216	46-47	0.2024542603041226
2216	48-49	0.19531581622568694
2216	50-51	0.14897069167620458
2216	52-53	0.21040853068467413
2216	54-55	0.18785294282006504
2216	56-57	0.18485422711721
2216	58-59	0.13085214087865893
2216	60-61	0.14803811313235826
2216	62-63	0.13131580273051213
2216	64-65	0.10738985896570341
2216	66-67	0.12096589127034463
2216	68-69	0.07400904049621815
2216	70-71	0.06707234656543548
2216	72-73	0.036044250051674
2216	74-75	0.003531406977621998
2216	76-77	-0.09903667306263131
2216	78-79	-0.10587788607658055
2216	80-81	-0.1113534071277833
2216	82-83	-0.24637384509427562
2216	84-85	-0.26659676794999854
2216	86-87	-0.28660643674310293
2216	88-89	-0.3086651526555073
2216	90-91	-0.3134902271312612
2216	92-93	-0.2359056858315789
2216	94-95	-0.22212982336339948
2216	96-97	-0.23683592052731228
2216	98-99	-0.15234711054436545
2216	100	0.1956093091395985
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	525.0
6	4148.0
7	12680.0
8	25580.0
9	42344.0
10	61560.0
11	74231.0
12	77804.0
13	80608.0
14	86817.0
15	96846.0
16	107566.0
17	121853.0
18	140748.0
19	158182.0
20	179074.0
21	203121.0
22	226862.0
23	264796.0
24	377808.0
25	508116.0
26	400292.0
27	801076.0
28	558647.0
29	911848.0
30	869171.0
31	1060376.0
32	1358478.0
33	2216299.0
34	2612475.0
35	5388547.0
36	2347360.0
37	861836.0
38	130486.0
39	3186.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.725780006433226	31.214049202340195	25.818677325825067	27.24149346540151
2	13.2198362649806	33.65276389340113	27.799251253885117	25.328148587733157
3	11.690052899348554	36.10599233761141	25.143602177302377	27.06035258573766
4	10.458053675075158	35.54724810552353	25.16328025235131	28.831417967050005
5	10.404922759924265	35.33176111431436	25.998315792522696	28.26500033323867
6	10.272471968375422	31.905006998518758	27.570520715544006	30.252000317561812
7	14.228386934296221	36.747746697092	22.872419720810676	26.1514466478011
8	12.457556769141322	33.90641594185206	20.51772639768368	33.11830089132293
9	9.780910434876246	32.73385834106681	21.77545542283473	35.709775801222214
10-11	14.35088294518522	47.472087274349484	20.274930471719504	17.902099308745793
12-13	13.985177110703258	48.665297176786005	19.52538441248084	17.8241413000299
14-15	15.241902268740626	47.523566614424055	19.101180952387764	18.133350164447556
16-17	15.180403215673222	47.250008191297574	19.758122716410394	17.811465876618808
18-19	15.784663247421749	45.72677415655545	20.80576985343306	17.682792742589744
20-21	14.981660683191237	45.42282344795248	21.572369284175487	18.0231465846808
22-23	15.338891010403177	46.27469069014516	22.092208883179403	16.29420941627226
24-25	15.409396561754669	46.208139489819864	21.524026731865526	16.858437216559945
26-27	17.058749195902458	46.39035689973691	20.368589239998137	16.182304664362498
28-29	16.493479406453424	47.28785290724258	21.085106028231728	15.133561658072267
30-31	16.826773524964498	46.98751199583359	20.968752878966537	15.216961600235374
32-33	17.36605873795119	47.087561184015506	20.354352401378808	15.192027676654495
34-35	17.45453465338037	47.11763595216805	20.264430929523886	15.163398464927692
36-37	18.00532552523903	46.84393713208046	19.97464878516638	15.176088557514134
38-39	18.422556384087553	47.04013447630444	19.92274675137965	14.61456238822836
40-41	18.710562177578858	47.333269888025434	19.8579082877661	14.098259646629602
42-43	18.98162845504637	47.589179494739604	19.979502142002918	13.449689908211113
44-45	19.168135348732786	47.3986017096275	19.87241882688929	13.560844114750417
46-47	19.163357243665867	47.03127960695574	20.204726420609763	13.600636728768626
48-49	18.997191940214222	47.121804093984395	20.60509834087359	13.275905624927795
50-51	19.182190277002913	46.87212928069214	20.971067328828973	12.974613113475971
52-53	19.276155971986462	46.852088555326965	21.23056189117095	12.641193581515623
54-55	19.248867759478962	46.97256953632053	21.252306057860316	12.526256646340197
56-57	19.051582470854814	47.11993864799632	20.8845368316414	12.943942049507463
58-59	18.784527880068122	46.963663111461045	21.016052177365456	13.235756831105375
60-61	18.803834074433926	46.60078655531857	21.325300765897197	13.27007860435031
62-63	18.94978792135933	45.92353940263777	21.577735277392566	13.548937398610331
64-65	18.780993793681358	45.704757121212396	21.65078952561279	13.863459559493455
66-67	18.332030920685796	45.45317255115505	22.04958611707088	14.165210411088278
68-69	18.037320499284696	45.54157488529568	21.80046603856647	14.620638576853151
70-71	17.52559462498236	45.727198553175576	21.969506688532277	14.777700133309784
72-73	17.395641829879313	46.27059967751429	21.947218891554616	14.386539601051778
74-75	17.095358538811873	46.91069225650716	21.770624238923542	14.223324965757426
76-77	16.433683341742928	47.83872369885807	21.778609638289677	13.948983321109324
78-79	15.895251767174395	48.81767668335556	21.43212926103759	13.854942288432454
80-81	15.297248025231125	50.11252241116988	20.910208747455812	13.680020816143184
82-83	14.756768429555557	51.1603418011499	20.722031081584923	13.360858687709626
84-85	14.186071460659894	52.36052708863498	20.499604625413312	12.953796825291816
86-87	13.735466542410856	53.22846224538469	20.144404914690426	12.891666297514023
88-89	13.404664395818783	53.85282144997342	19.98429580039163	12.758218353816172
90-91	13.295619919739702	54.31546895656896	19.6721164916204	12.716794632070938
92-93	13.330527937801396	54.510880227714445	19.264227805269254	12.894364029214914
94-95	13.443922704629468	54.58085558450772	18.884990398927012	13.090231311935804
96-97	13.619940739895739	54.25797563171159	18.709574709561924	13.412508918830751
98-99	13.897901934605786	53.693188980369285	18.545982804020657	13.862926281004267
100	14.471581231701599	52.51070474744055	18.31841538402878	14.69929863682907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1930584.0
1	1901385.0
2	1196080.5
3	322143.5
4	121807.0
5	116057.5
6	100240.5
7	73314.0
8	49943.5
9	38324.0
10	36895.5
11	41726.0
12	51700.5
13	67176.0
14	88296.0
15	114839.5
16	145955.0
17	179817.0
18	215416.5
19	251223.5
20	284438.0
21	313664.5
22	338063.0
23	357169.5
24	371763.5
25	380712.5
26	382853.5
27	378172.0
28	366600.5
29	348840.5
30	327727.0
31	305106.5
32	281434.5
33	258522.0
34	236921.5
35	217892.5
36	201669.0
37	188420.0
38	179994.0
39	177008.0
40	180845.5
41	194951.5
42	223707.5
43	270519.5
44	336942.0
45	421029.0
46	512115.0
47	595242.5
48	658198.0
49	698518.0
50	723529.0
51	741005.5
52	754214.0
53	754784.5
54	725670.0
55	655768.5
56	548025.5
57	418867.0
58	292966.0
59	190546.0
60	117836.0
61	72160.5
62	46278.0
63	31933.5
64	24003.5
65	19524.5
66	16534.0
67	14280.0
68	12473.5
69	10974.5
70	9785.0
71	8779.0
72	7995.5
73	7326.0
74	6735.5
75	6254.5
76	5902.0
77	5562.5
78	5206.0
79	4803.5
80	4521.5
81	4306.5
82	4016.5
83	3834.0
84	3784.0
85	3710.5
86	3546.0
87	3438.0
88	3357.5
89	3291.5
90	3206.0
91	3066.0
92	2977.0
93	2878.5
94	2698.0
95	2571.0
96	2432.0
97	2213.0
98	1906.5
99	1537.0
100	1178.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013892767236742454
2	3.799501979160581E-4
3	0.0
4	4.470002328424213E-6
5	0.0
6	0.004045352107223913
7	3.6654019093078545E-4
8	0.0
9	0.0
10-11	2.7043514086966486E-4
12-13	2.682001397054528E-5
14-15	3.888902025729065E-4
16-17	2.2350011642121066E-4
18-19	1.765650919727564E-4
20-21	8.046004191163584E-5
22-23	7.152003725478741E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	1.9668010245066535E-4
32-33	3.352501746318159E-5
34-35	1.2963006752430217E-4
36-37	1.720950896443322E-4
38-39	5.609852922172386E-4
40-41	0.0016941308824727766
42-43	0.00105045054717969
44-45	4.715852456487545E-4
46-47	5.900403073519961E-4
48-49	9.498754947901453E-4
50-51	0.0
52-53	1.2516006519587797E-4
54-55	5.02875261947724E-4
56-57	5.341652782466934E-4
58-59	5.364002794109055E-4
60-61	3.7995019791605805E-5
62-63	2.3467512224227118E-4
64-65	1.765650919727564E-4
66-67	2.2797011874963488E-4
68-69	1.2739506636009007E-4
70-71	6.794403539204803E-4
72-73	5.766303003667234E-4
74-75	3.531301839455128E-4
76-77	3.6207018860236125E-4
78-79	8.40360437743752E-4
80-81	0.00113538059141975
82-83	4.470002328424213E-4
84-85	7.286103795331466E-4
86-87	0.001341000698527264
88-89	0.001193490621689265
90-91	0.0011957256228534768
92-93	7.867204098026614E-4
94-95	6.950853620699651E-4
96-97	6.570903422783592E-4
98-99	3.441901792886644E-4
100	9.118804749985395E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.2371353E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.83152499331679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.96641624326689	71.06481750633752
2	4.39225091552989	6.57357668324787
3	0.37711376097989663	0.8465999349027248
4	0.08429690213857276	0.25232262956967144
5	0.03621157948912369	0.13548838577939185
6	0.027707674767185775	0.12440445341084132
7	0.019271346523173985	0.10094729743026337
8	0.013059746278384356	0.0781824584029836
9	0.011027984324736947	0.07427167961602128
>10	0.06299776532430942	0.8434341626675532
>50	0.004724965218714717	0.2483428065241662
>100	0.0031960666151899983	0.47646681199757807
>500	4.477256142189668E-4	0.23713353562028355
>1k	7.343335380454926E-4	1.223945282364622
>5k	1.4320625715530437E-4	0.7953312274847116
>10k+	3.997841345585479E-4	16.924735144643794
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	909062	4.063509256681972	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	239246	1.0694301770661794	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	216515	0.9678225541387685	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	187760	0.8392876371849302	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	142096	0.635169450859767	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	102680	0.4589798390825982	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	102164	0.4566733178811313	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	97515	0.4358922770562871	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	96698	0.43224028515396457	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81169	0.36282561899586496	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	80274	0.3588249669119253	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77379	0.3458843101711372	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	74272	0.3319960129367231	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65554	0.2930265326375209	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61928	0.27681830419465464	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55145	0.24649827840095323	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54068	0.24168408589324034	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53270	0.23811702403515783	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52222	0.23343246159496922	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51386	0.22969553964840658	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48366	0.21619613261656548	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48254	0.21569549235578195	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39634	0.17716407228476524	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37552	0.16785752743698604	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36920	0.16503248596542194	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35845	0.16022723346236592	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35088	0.1568434416997488	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34915	0.1560701312969314	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33146	0.14816269717794894	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32641	0.14590534600209473	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25574	0.11431583954712081	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24646	0.11016767738634314	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24166	0.10802207626869954	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23329	0.10428068431980846	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22604	0.10103993263170091	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002167951129285743	0.0	0.0	4.470002328424213E-6	0.0
2	0.005498102863961782	0.0	0.0	1.341000698527264E-5	0.0
3	0.009610505006112057	0.0	0.0	3.5760018627393705E-5	0.0
4	0.015779108219337472	0.0	0.0	5.811003026951477E-5	0.0
5	0.025841083460620376	0.0	0.0	7.152003725478741E-5	0.0
6	0.044172563009488075	0.0	0.0	7.152003725478741E-5	0.0
7	0.0658520743023455	0.0	0.0	7.599003958321162E-5	0.0
8	0.1425796642697471	0.0	0.0	7.599003958321162E-5	0.0
9	0.4260314519197833	0.0	0.0	7.599003958321162E-5	0.0
10-11	1.060344897333657	0.0	0.0	7.822504074742374E-5	0.0
12-13	1.9138985469497531	0.0	0.0	8.046004191163584E-5	0.0
14-15	2.9188198854132783	0.0	0.0	8.940004656848426E-5	0.0
16-17	4.035748307221294	0.0	0.0	1.1398505937481744E-4	0.0
18-19	5.277105948844488	0.0	0.0	1.2739506636009007E-4	0.0
20-21	6.887410877652327	0.0	0.0	1.341000698527264E-4	0.0
22-23	8.672148707322261	4.470002328424213E-6	4.470002328424213E-6	1.341000698527264E-4	0.0
24-25	10.454564370782581	4.470002328424213E-6	4.470002328424213E-6	1.408050733453627E-4	0.0
26-27	12.187029993223923	4.470002328424213E-6	4.470002328424213E-6	1.4751007683799902E-4	0.0
28-29	13.961276727429048	4.470002328424213E-6	4.470002328424213E-6	1.5198007916642322E-4	0.0
30-31	15.807982199377928	4.470002328424213E-6	4.470002328424213E-6	1.5645008149484745E-4	0.0
32-33	17.864674970709192	4.470002328424213E-6	4.470002328424213E-6	1.67625087315908E-4	0.0
34-35	19.76958210797532	4.470002328424213E-6	4.470002328424213E-6	1.765650919727564E-4	0.0
36-37	21.325198793296053	4.470002328424213E-6	4.470002328424213E-6	1.9891510361487748E-4	0.0
38-39	22.543176087740424	4.470002328424213E-6	4.470002328424213E-6	2.5479013272018013E-4	0.0
40-41	23.603637205134618	4.470002328424213E-6	4.470002328424213E-6	2.816101466907254E-4	0.0
42-43	24.472344162644074	8.940004656848426E-6	4.470002328424213E-6	3.2631016997496754E-4	0.0
44-45	25.18620800449575	8.940004656848426E-6	4.470002328424213E-6	3.598351874381491E-4	0.0
46-47	25.80195306023735	8.940004656848426E-6	4.470002328424213E-6	3.9336020490133075E-4	0.0
48-49	26.37066028147694	8.940004656848426E-6	4.470002328424213E-6	4.157102165434518E-4	0.0
50-51	26.94775546208582	8.940004656848426E-6	4.470002328424213E-6	4.7158524564875444E-4	0.0
52-53	27.563605562882138	8.940004656848426E-6	4.470002328424213E-6	5.587502910530266E-4	0.0
54-55	28.206508117770078	8.940004656848426E-6	4.470002328424213E-6	6.213303236509656E-4	0.0
56-57	28.891448809555683	8.940004656848426E-6	4.470002328424213E-6	6.548553411141471E-4	0.0
58-59	29.60291449515816	8.940004656848426E-6	4.470002328424213E-6	6.92850360905753E-4	0.0
60-61	30.326554232102097	8.940004656848426E-6	4.470002328424213E-6	7.487253900110557E-4	0.0
62-63	31.01560732602986	8.940004656848426E-6	4.470002328424213E-6	7.844854086384494E-4	0.0
64-65	31.657776800535935	8.940004656848426E-6	4.470002328424213E-6	8.761204563711457E-4	0.0
66-67	32.23431770085609	1.1175005821060533E-5	4.470002328424213E-6	9.476404936259331E-4	0.0
68-69	32.742315585472184	1.5645008149484746E-5	4.470002328424213E-6	0.0010012805215670237	0.0
70-71	33.20135353458505	1.7880009313696852E-5	4.470002328424213E-6	0.0010415105425228416	0.0
72-73	33.63118001848167	1.7880009313696852E-5	4.470002328424213E-6	0.001068330556493387	0.0
74-75	34.03732890004463	1.7880009313696852E-5	4.470002328424213E-6	0.0010839755646428716	0.0
76-77	34.43003871960717	1.7880009313696852E-5	4.470002328424213E-6	0.0011465555972408107	0.0
78-79	34.81129639320429	1.7880009313696852E-5	4.470002328424213E-6	0.0012672456601082642	0.0
80-81	35.17928262988832	1.7880009313696852E-5	4.470002328424213E-6	0.0014460457532452329	0.0
82-83	35.54796842193675	1.7880009313696852E-5	4.470002328424213E-6	0.001513095788171596	0.0
84-85	35.92085378117274	1.7880009313696852E-5	4.470002328424213E-6	0.0015443858044705656	0.0
86-87	36.2996730684997	1.7880009313696852E-5	4.470002328424213E-6	0.001555560810291626	0.0
88	36.58402332661775	1.7880009313696852E-5	4.470002328424213E-6	0.001555560810291626	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	9885	0.0	48.924973	9
GCTTGCT	2215	0.0	47.31774	7
GCCTGCT	2130	0.0	42.365707	7
CTTGCTG	7085	0.0	40.92957	8
CCTGCTG	7210	0.0	38.785873	8
CTGCTGA	9485	0.0	37.510292	9
GTTGCTG	2500	0.0	36.847492	8
TGCTTGC	1210	0.0	34.960304	6
ATTGCTG	1780	0.0	31.949	8
TACCCGA	15975	0.0	31.92324	6
ACTTGCT	1730	0.0	31.242414	7
TTTGCTG	3355	0.0	31.23951	8
ACCCGAC	17550	0.0	30.904486	7
TCGCGAC	1050	0.0	30.887154	6
GACACGC	1340	0.0	30.51628	2
TGCCAAA	2465	0.0	30.317844	6
CCCGACG	19435	0.0	30.035091	8
ACTGCTG	1480	0.0	29.85094	8
TGACGAC	1380	0.0	28.950623	6
CTACCCG	16565	0.0	28.402773	5
>>END_MODULE