##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248486_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22371353
Sequences flagged as poor quality	0
Sequence length	100
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25233431344095	34.0	31.0	34.0	30.0	34.0
2	32.47249895882471	34.0	31.0	34.0	31.0	34.0
3	32.623134416590716	34.0	31.0	34.0	31.0	34.0
4	36.14409074855687	37.0	35.0	37.0	35.0	37.0
5	36.056968525774906	37.0	35.0	37.0	35.0	37.0
6	36.04886208715226	37.0	35.0	37.0	35.0	37.0
7	36.05156558926051	37.0	35.0	37.0	35.0	37.0
8	36.04053787001617	37.0	35.0	37.0	35.0	37.0
9	37.8130808181338	39.0	38.0	39.0	35.0	39.0
10-11	37.86290196663563	39.0	38.0	39.0	35.0	39.0
12-13	37.83428427417868	39.0	38.0	39.0	35.0	39.0
14-15	39.268573966000176	41.0	39.0	41.0	36.0	41.0
16-17	39.19126064927767	41.0	39.0	41.0	36.0	41.0
18-19	38.946603386035704	40.0	38.0	41.0	35.5	41.0
20-21	38.54373982208408	40.0	37.5	41.0	35.0	41.0
22-23	38.146716651424704	40.0	36.0	41.0	34.0	41.0
24-25	37.77891882533882	39.0	35.0	41.0	34.0	41.0
26-27	37.57221360281606	39.0	35.0	41.0	33.0	41.0
28-29	37.439962772926606	39.0	35.0	41.0	33.0	41.0
30-31	37.341340441054236	39.0	35.0	41.0	33.0	41.0
32-33	37.339649349773346	39.0	35.0	41.0	33.0	41.0
34-35	37.27544503007931	39.0	35.0	41.0	33.0	41.0
36-37	37.249730738234746	39.0	35.0	41.0	33.0	41.0
38-39	37.15011546239514	39.0	35.0	41.0	33.0	41.0
40-41	37.110960432299294	39.0	35.0	41.0	33.0	41.0
42-43	37.04394430234059	39.0	35.0	41.0	33.0	41.0
44-45	36.88289204054846	38.5	35.0	41.0	33.0	41.0
46-47	36.6675582607811	38.0	35.0	40.5	33.0	41.0
48-49	36.38938150052883	37.0	35.0	40.0	32.0	41.0
50-51	36.064814944362105	36.5	35.0	40.0	32.0	41.0
52-53	35.72494256382258	36.0	35.0	39.0	31.5	41.0
54-55	35.38487506768142	35.0	35.0	39.0	31.0	41.0
56-57	35.031814593422226	35.0	35.0	38.5	31.0	40.5
58-59	34.75249516647473	35.0	34.5	37.0	30.5	40.0
60-61	34.49304800652871	35.0	34.0	37.0	30.0	40.0
62-63	34.25412434822337	35.0	34.0	36.0	30.0	39.5
64-65	34.0392706914061	35.0	34.0	35.5	30.0	39.0
66-67	33.84711199631064	35.0	34.0	35.0	29.0	39.0
68-69	33.68021022242151	35.0	33.5	35.0	29.0	38.5
70-71	33.52899080355131	35.0	33.0	35.0	29.0	37.5
72-73	33.37411664372736	35.0	33.0	35.0	29.0	37.0
74-75	33.233844104109394	35.0	33.0	35.0	29.0	36.5
76-77	33.101868469913285	35.0	33.0	35.0	29.0	36.0
78-79	32.98533065925874	35.0	33.0	35.0	29.0	36.0
80-81	32.881173570503314	35.0	33.0	35.0	29.0	35.5
82-83	32.76723178075103	35.0	33.0	35.0	28.0	35.0
84-85	32.63834713081502	35.0	33.0	35.0	27.0	35.0
86-87	32.531386501299224	35.0	33.0	35.0	27.0	35.0
88-89	32.416535267223225	35.0	33.0	35.0	27.0	35.0
90-91	32.30811810979873	35.0	33.0	35.0	27.0	35.0
92-93	32.137721509289136	35.0	33.0	35.0	25.5	35.0
94-95	31.917015211373222	35.0	33.0	35.0	25.0	35.0
96-97	31.67886980282328	35.0	32.0	35.0	24.0	35.0
98-99	31.264263721554972	34.5	32.0	35.0	22.0	35.0
100	29.54884136869147	33.0	29.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.22993770330421626
1101	2	0.2601812031156996
1101	3	0.24683423617092615
1101	4	0.181517150680655
1101	5	0.1889521925857025
1101	6	0.19163495531682884
1101	7	0.21501205079299268
1101	8	0.222702340175978
1101	9	0.27083924818104066
1101	10-11	0.2728294385745542
1101	12-13	0.2974358049395178
1101	14-15	0.3911657288888364
1101	16-17	0.4000684355315727
1101	18-19	0.4303693383033931
1101	20-21	0.43817682049617446
1101	22-23	0.39551948679635274
1101	24-25	0.41079031903740315
1101	26-27	0.4352386966468913
1101	28-29	0.4622634389321618
1101	30-31	0.4695781477996448
1101	32-33	0.46818569522758935
1101	34-35	0.45617196530562154
1101	36-37	0.44119986318640514
1101	38-39	0.4921828608076524
1101	40-41	0.46452005036103117
1101	42-43	0.4519650710488179
1101	44-45	0.4427920990896723
1101	46-47	0.4176572760901678
1101	48-49	0.40275041019237534
1101	50-51	0.38332623739623983
1101	52-53	0.37298400223021844
1101	54-55	0.39345841475120125
1101	56-57	0.379132269116333
1101	58-59	0.38838383213796845
1101	60-61	0.39666757021894483
1101	62-63	0.39741331942443026
1101	64-65	0.35192230413835546
1101	66-67	0.36691172062403865
1101	68-69	0.34640783918641205
1101	70-71	0.3710528415153007
1101	72-73	0.39394828008936855
1101	74-75	0.4206002722718907
1101	76-77	0.4061053724304955
1101	78-79	0.40739255242559125
1101	80-81	0.40484569806295667
1101	82-83	0.41398083452064327
1101	84-85	0.39917244881764447
1101	86-87	0.42469244659501726
1101	88-89	0.37223138018713087
1101	90-91	0.40359591230676983
1101	92-93	0.39117901961395063
1101	94-95	0.3751491753111864
1101	96-97	0.4407473701327227
1101	98-99	0.5431697705848322
1101	100	0.9804009865130396
1102	1	0.2039824641327712
1102	2	0.26341977931605953
1102	3	0.2403699195001323
1102	4	0.14403124626859665
1102	5	0.15468873819137485
1102	6	0.14893780384098676
1102	7	0.2015843951012215
1102	8	0.1999528467871201
1102	9	0.2394815006767459
1102	10-11	0.2673942628643857
1102	12-13	0.28453072353193676
1102	14-15	0.3523974668763117
1102	16-17	0.3774899122867481
1102	18-19	0.3869606708952418
1102	20-21	0.402615238043353
1102	22-23	0.36900656776757756
1102	24-25	0.38864659502753085
1102	26-27	0.4199323777326498
1102	28-29	0.4163658775429866
1102	30-31	0.4366673140285755
1102	32-33	0.4417576609855445
1102	34-35	0.4499243738577192
1102	36-37	0.43073706101316844
1102	38-39	0.4366198465632536
1102	40-41	0.4006896402935496
1102	42-43	0.41402394288004984
1102	44-45	0.44371945093333665
1102	46-47	0.4137807522548371
1102	48-49	0.4089286427509009
1102	50-51	0.4059609873633363
1102	52-53	0.3455037642148753
1102	54-55	0.3595717171918338
1102	56-57	0.3930113319361084
1102	58-59	0.4084110836080015
1102	60-61	0.43751644328335004
1102	62-63	0.37142215206666407
1102	64-65	0.35949356170787894
1102	66-67	0.3514702485881429
1102	68-69	0.360040570119871
1102	70-71	0.38049878263813497
1102	72-73	0.38439224960851703
1102	74-75	0.35860161424301396
1102	76-77	0.3516454157577513
1102	78-79	0.3483461641569079
1102	80-81	0.35127539383540807
1102	82-83	0.3562378731250675
1102	84-85	0.3739768595657367
1102	86-87	0.3704520609420996
1102	88-89	0.3978049638992047
1102	90-91	0.42519170924255434
1102	92-93	0.3560330569104906
1102	94-95	0.3658299610831506
1102	96-97	0.41980051865129653
1102	98-99	0.5992142951529544
1102	100	0.9145617402460751
1103	1	0.17545770433233088
1103	2	0.1748085372495467
1103	3	0.19887545171443577
1103	4	0.10073906412257827
1103	5	0.13197313667281918
1103	6	0.13820747601324967
1103	7	0.15921189161754512
1103	8	0.15883382473047192
1103	9	0.1608381717587264
1103	10-11	0.19635014366787118
1103	12-13	0.2447479529137624
1103	14-15	0.31791434722033074
1103	16-17	0.3350400639343931
1103	18-19	0.3253703383052553
1103	20-21	0.348323367968554
1103	22-23	0.3112943812369977
1103	24-25	0.3101160383675037
1103	26-27	0.32734444032344356
1103	28-29	0.32118805398849304
1103	30-31	0.35531840531222514
1103	32-33	0.327968777270776
1103	34-35	0.33577302085551963
1103	36-37	0.3303604963951585
1103	38-39	0.3643998984361261
1103	40-41	0.34610354461296566
1103	42-43	0.335265692582702
1103	44-45	0.329047907491649
1103	46-47	0.34217612362856187
1103	48-49	0.3264892002261419
1103	50-51	0.3028805258200933
1103	52-53	0.3025135918305679
1103	54-55	0.3231131822448532
1103	56-57	0.3247320920508514
1103	58-59	0.2879863290030471
1103	60-61	0.32409138380652536
1103	62-63	0.2976732055847435
1103	64-65	0.2522104277044832
1103	66-67	0.26720176120981165
1103	68-69	0.3140862504415338
1103	70-71	0.2729399234173755
1103	72-73	0.27978734858217535
1103	74-75	0.29434633274529176
1103	76-77	0.2625517109058606
1103	78-79	0.2447456350116255
1103	80-81	0.2487125741215621
1103	82-83	0.2467784530861863
1103	84-85	0.21907714705238135
1103	86-87	0.23165838496448288
1103	88-89	0.2444525797588497
1103	90-91	0.2840016951497901
1103	92-93	0.2922179164233327
1103	94-95	0.2671603868219492
1103	96-97	0.32534256262121275
1103	98-99	0.4616283618100674
1103	100	0.8568686859156891
1104	1	0.19899518596911747
1104	2	0.1883699714218494
1104	3	0.18595899605185195
1104	4	0.12040115945350038
1104	5	0.12082883659449095
1104	6	0.12269901430043717
1104	7	0.17289591126405668
1104	8	0.16216377714953012
1104	9	0.19505413935758753
1104	10-11	0.2079945464516868
1104	12-13	0.21624719760863087
1104	14-15	0.2810672149105855
1104	16-17	0.2932449587106234
1104	18-19	0.2548676292798788
1104	20-21	0.30460400336784943
1104	22-23	0.27374368540385063
1104	24-25	0.26047389773842866
1104	26-27	0.2519822931777185
1104	28-29	0.2680498805164717
1104	30-31	0.2759233027207699
1104	32-33	0.28977179090182403
1104	34-35	0.2811620308253211
1104	36-37	0.2757475889822416
1104	38-39	0.28265603731161804
1104	40-41	0.2603385196439518
1104	42-43	0.2657008034641848
1104	44-45	0.25080664085258064
1104	46-47	0.2620274523339958
1104	48-49	0.2345133906038157
1104	50-51	0.2338149410016399
1104	52-53	0.2307673325965922
1104	54-55	0.23341021257882488
1104	56-57	0.24315237078150176
1104	58-59	0.22190827695194315
1104	60-61	0.21671915788517282
1104	62-63	0.19367656115367993
1104	64-65	0.1762273862710444
1104	66-67	0.19401509644362847
1104	68-69	0.2080813890618387
1104	70-71	0.20538551289212847
1104	72-73	0.20189841764897665
1104	74-75	0.257680047327284
1104	76-77	0.2562847026841766
1104	78-79	0.24587293960924228
1104	80-81	0.22513876100592967
1104	82-83	0.24286136219430432
1104	84-85	0.20987182543873217
1104	86-87	0.19335351406350298
1104	88-89	0.18167487511279035
1104	90-91	0.18876143612357055
1104	92-93	0.20626614220937967
1104	94-95	0.19326679202366392
1104	96-97	0.19924633698776262
1104	98-99	0.3604433186899705
1104	100	0.758526882937403
1105	1	0.14275624448086432
1105	2	0.1733797165756812
1105	3	0.15460525918422974
1105	4	0.1045060194699019
1105	5	0.08521882630520139
1105	6	0.09836461278653985
1105	7	0.15257927484517353
1105	8	0.1251110450245818
1105	9	0.1722871478141954
1105	10-11	0.15633839961117246
1105	12-13	0.16242856821433094
1105	14-15	0.22752692108849004
1105	16-17	0.2464887210610769
1105	18-19	0.26113088273619667
1105	20-21	0.2701600830276618
1105	22-23	0.2520523073691834
1105	24-25	0.2467434115975422
1105	26-27	0.27192701013036924
1105	28-29	0.2700778590997004
1105	30-31	0.27210030732054946
1105	32-33	0.2542654884046698
1105	34-35	0.25397813934838354
1105	36-37	0.22305074508609124
1105	38-39	0.28391218496621917
1105	40-41	0.260155109450551
1105	42-43	0.26888446303289015
1105	44-45	0.2673121597606922
1105	46-47	0.26416464454614186
1105	48-49	0.24648435657294954
1105	50-51	0.23564923170170005
1105	52-53	0.20348344971943533
1105	54-55	0.2242796839460297
1105	56-57	0.2311114684202309
1105	58-59	0.22051012115679214
1105	60-61	0.2248880637612558
1105	62-63	0.23566593088531818
1105	64-65	0.21008148977364272
1105	66-67	0.20488435367153102
1105	68-69	0.22899273825861854
1105	70-71	0.22521483053375846
1105	72-73	0.21031214928677855
1105	74-75	0.2053501498216761
1105	76-77	0.21378888364424853
1105	78-79	0.2252665007979715
1105	80-81	0.22868861573371646
1105	82-83	0.21914805902645185
1105	84-85	0.21170791133288702
1105	86-87	0.23239517654549502
1105	88-89	0.22019220246168203
1105	90-91	0.23722349718408253
1105	92-93	0.24321815901142685
1105	94-95	0.25274440880839677
1105	96-97	0.27938734148689903
1105	98-99	0.41777424507127847
1105	100	0.8475463788687669
1106	1	0.11462921880686139
1106	2	0.14081751095201156
1106	3	0.13705160366292546
1106	4	0.10530664943849644
1106	5	0.07494844368256537
1106	6	0.06384313709362033
1106	7	0.09632063381327782
1106	8	0.12411849029779631
1106	9	0.11478799914232951
1106	10-11	0.12416435314868579
1106	12-13	0.1380194332697826
1106	14-15	0.14950485758516407
1106	16-17	0.17978088504069234
1106	18-19	0.1437410707971125
1106	20-21	0.1695373974885257
1106	22-23	0.13238472662158784
1106	24-25	0.15718452833569785
1106	26-27	0.1888551774108791
1106	28-29	0.15434582416594367
1106	30-31	0.19768287120345462
1106	32-33	0.16747269931305198
1106	34-35	0.1713049395300814
1106	36-37	0.13212480839195706
1106	38-39	0.16980930158778307
1106	40-41	0.12137488020361786
1106	42-43	0.158562831774276
1106	44-45	0.14339373226632546
1106	46-47	0.131605774807511
1106	48-49	0.0890848065372083
1106	50-51	0.09616792546576391
1106	52-53	0.09528926772771484
1106	54-55	0.07252964754741242
1106	56-57	0.09443896878843105
1106	58-59	0.10119739755965895
1106	60-61	0.11644900697601202
1106	62-63	0.09492449347668241
1106	64-65	0.0954584194219521
1106	66-67	0.10269045417364708
1106	68-69	0.09884882876601608
1106	70-71	0.1070506092517789
1106	72-73	0.07700250347289028
1106	74-75	0.11817684719112975
1106	76-77	0.07118102622962397
1106	78-79	0.10887451534503612
1106	80-81	0.07954699115323649
1106	82-83	0.06868517618835313
1106	84-85	0.09982679956941354
1106	86-87	0.08447757611475026
1106	88-89	0.08264344335491103
1106	90-91	0.08132688840373703
1106	92-93	0.10275957820254433
1106	94-95	0.08962340558342419
1106	96-97	0.09057037508282306
1106	98-99	0.2904258075152093
1106	100	0.6643992321721086
1107	1	0.10398151671981992
1107	2	0.1043441620538701
1107	3	0.09220752835118162
1107	4	0.06376750068549342
1107	5	-6.460515447983539E-4
1107	6	0.05656993406886812
1107	7	0.09353623831773206
1107	8	0.09343334867410391
1107	9	0.1201512964192375
1107	10-11	0.11898163060769917
1107	12-13	0.13828259403236132
1107	14-15	0.1678984998124875
1107	16-17	0.11948894691037282
1107	18-19	0.12540901599133036
1107	20-21	0.14987414784168607
1107	22-23	0.1672486937614437
1107	24-25	0.16057688154647565
1107	26-27	0.19929259715506475
1107	28-29	0.18419049982460933
1107	30-31	0.2172845199813338
1107	32-33	0.17886665962402049
1107	34-35	0.1719765703627445
1107	36-37	0.15928277514547773
1107	38-39	0.19543127053555054
1107	40-41	0.18258424302577225
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2216	42-43	-0.5786054767906421
2216	44-45	-0.55221567148471
2216	46-47	-0.5094805140014813
2216	48-49	-0.5027032319032614
2216	50-51	-0.47441969305700127
2216	52-53	-0.44774641462622355
2216	54-55	-0.4238282339106192
2216	56-57	-0.42815002003918323
2216	58-59	-0.4352918785759101
2216	60-61	-0.3966571843080615
2216	62-63	-0.39870937538627516
2216	64-65	-0.4313582091530108
2216	66-67	-0.43138645009995713
2216	68-69	-0.45337805772615525
2216	70-71	-0.45432550726182086
2216	72-73	-0.4816935028912184
2216	74-75	-0.48812933145998727
2216	76-77	-0.4724200551492217
2216	78-79	-0.4775399351963898
2216	80-81	-0.495120527735466
2216	82-83	-0.48856205994330537
2216	84-85	-0.5617403815136228
2216	86-87	-0.5557576165597169
2216	88-89	-0.5876997806729634
2216	90-91	-0.5599849733719964
2216	92-93	-0.5555461636627186
2216	94-95	-0.5562354003445833
2216	96-97	-0.6285513879509033
2216	98-99	-0.4771639440028892
2216	100	0.1080039938974906
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	14.0
7	42.0
8	118.0
9	349.0
10	741.0
11	1500.0
12	2459.0
13	3695.0
14	5711.0
15	9719.0
16	15917.0
17	25575.0
18	36215.0
19	47645.0
20	60452.0
21	76272.0
22	95146.0
23	114450.0
24	137332.0
25	163983.0
26	196844.0
27	236835.0
28	288661.0
29	364420.0
30	423592.0
31	526896.0
32	688156.0
33	990191.0
34	1400168.0
35	5256774.0
36	3458059.0
37	5383506.0
38	2042906.0
39	316539.0
40	470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.060596005424124	22.158847480411406	32.04988527764928	25.73067123651519
2	16.903499819050722	23.21366455919418	38.15555068568969	21.72728493606541
3	13.864615162078037	19.984562399958556	42.64072897155572	23.510093466407685
4	14.11000402926496	17.0854155001809	46.29002768990984	22.514552780644294
5	12.521660809960466	18.564060906405626	48.43820820205976	20.476070081574147
6	11.42233158805244	14.00915462268483	44.631483278368556	29.937030510894175
7	15.671917563501857	14.792797735568342	42.66428588382652	26.870998817103285
8	11.53615071918091	14.441316982482016	43.94086490879653	30.081667389540545
9	11.688743188666328	13.182385526704621	46.97328319838322	28.155588086245835
10-11	15.5160865773295	20.316089062650793	46.927550604561105	17.2402737554586
12-13	13.837327605179539	27.219437024232178	42.10115706885843	16.842078301729856
14-15	15.494715227813591	25.190172276693882	41.73142674790756	17.58368574758496
16-17	20.7713392352272	25.68094683602018	42.07163179691883	11.476082131833797
18-19	20.743544673930643	20.72291561272391	41.57880932768816	16.95473038565728
20-21	15.777041013224324	19.9067861048622	46.48172873599225	17.83444414592122
22-23	15.582597355766573	21.19950868101253	48.01530958797735	15.202584375243546
24-25	15.769423861611243	22.59548519651961	46.05698636686644	15.578104575002708
26-27	19.420290464043703	22.416214977150595	42.57250425822491	15.590990300580792
28-29	17.699949044698737	24.963238559631897	44.92371612778869	12.413096267880677
30-31	17.88266073064134	22.88535300099915	44.996750076199596	14.235236192159912
32-33	18.53299327522568	23.685933484560834	44.5260487247698	13.255024515443683
34-35	18.53583370918302	23.795539677646246	44.56986028896592	13.098766324204814
36-37	18.74173246424151	22.9073814275788	44.26993613770053	14.080949970479153
38-39	19.201131075698218	23.006035537256597	43.51156556326157	14.281267823783617
40-41	19.190813846282794	23.42726901908194	43.65385634062526	13.728060794010002
42-43	19.181555409359085	23.94748040037727	43.7132907299313	13.157673460332342
44-45	19.504099868441227	23.252367096806783	43.31833870282067	13.925194331931321
46-47	19.93778598829712	22.75240404577051	43.58576485438099	13.724045111551375
48-49	19.177193810585415	22.715662834958906	43.902700042071544	14.204443312384132
50-51	19.061206570218726	22.41524819099761	43.999849260976454	14.523695977807211
52-53	18.682955505615407	22.774225822633436	44.02173674961148	14.521081922139679
54-55	18.480532144131427	22.979733389739486	44.247877684235384	14.291856781893697
56-57	18.351327289314344	22.986524783255366	43.97976200736483	14.682385920065459
58-59	18.112369012143343	23.096319948847547	44.657677498536486	14.133633540472623
60-61	17.601914651778944	22.539790466713136	45.12571351203736	14.732581369470566
62-63	17.47274852698954	22.26541255234495	45.33275865772593	14.929080262939573
64-65	17.549605658848	22.232017211742967	45.433879205002306	14.784497924406729
66-67	17.18550334521543	21.922350838655362	46.1806033654938	14.711542450635406
68-69	17.102412869255936	21.48577886698589	46.84233176159325	14.569476502164935
70-71	16.764076089880497	21.229052339704836	47.33512123596803	14.671750334446632
72-73	16.47238567211844	20.8593125910535	47.91438255277471	14.753919184053348
74-75	16.46528503327093	20.476629883481607	48.13810731341417	14.919977769833286
76-77	16.566162916048192	20.29236981985986	48.4922180042784	14.649249259813551
78-79	16.650704529406116	20.207191797624404	48.87417030843087	14.26793336453861
80-81	16.436543556329024	19.882937583243663	49.2318678544895	14.44865100593781
82-83	16.378375398866986	19.883236996080196	49.18786886450731	14.550518740545504
84-85	16.193007511446073	20.112067072156993	48.98486601889961	14.71005939749733
86-87	16.171746063882804	20.392419368094707	48.72974519891681	14.706089369105676
88-89	16.057450573469044	21.045532182605815	48.416493098482555	14.480524145442592
90-91	16.030648274167692	21.681322963912507	48.08115588400141	14.20687287791839
92-93	15.800591731345046	22.26541515229395	47.60185545591938	14.332137660441624
94-95	15.74000943781228	23.14605469340286	47.065493245267945	14.048442623516916
96-97	15.239032735884392	24.211853111238806	47.03125283232614	13.517861320550667
98-99	14.94163831732713	25.131328042254868	46.73084190219473	13.196191738223268
100	14.656410838755512	26.03660640485152	46.24496043873892	13.062022317654051
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	511114.0
1	1165137.5
2	1767051.0
3	1184966.5
4	392675.5
5	91048.0
6	41777.5
7	28714.5
8	25432.0
9	26666.0
10	31029.5
11	39136.5
12	51872.0
13	70117.5
14	93933.0
15	122449.5
16	155675.5
17	192381.0
18	229877.5
19	264573.0
20	293291.5
21	317082.0
22	336451.5
23	351465.0
24	362705.5
25	369587.5
26	370992.5
27	365232.5
28	352915.0
29	336003.5
30	315946.0
31	293118.5
32	268874.0
33	244996.5
34	222251.0
35	200202.0
36	179046.5
37	159079.5
38	140554.5
39	124823.5
40	111436.5
41	101835.0
42	98115.5
43	104405.5
44	128578.0
45	175035.0
46	243305.0
47	328865.5
48	431096.5
49	558179.0
50	713645.5
51	888717.5
52	1052894.5
53	1155462.0
54	1152002.0
55	1031994.5
56	825773.5
57	589967.0
58	379932.5
59	223501.0
60	121477.5
61	61404.5
62	29180.0
63	13388.0
64	6169.0
65	3070.5
66	1873.0
67	1364.5
68	996.5
69	750.5
70	579.5
71	417.0
72	313.0
73	252.5
74	200.0
75	150.5
76	100.5
77	63.0
78	45.0
79	28.5
80	19.5
81	18.5
82	13.5
83	8.0
84	7.0
85	6.0
86	3.5
87	2.5
88	3.0
89	4.0
90	5.0
91	3.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01730784901565855
2	0.11314469893707367
3	0.0
4	1.2069006286745374E-4
5	0.037480969523837025
6	0.0016092008382327166
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.498754947901453E-4
14-15	8.493004424006005E-4
16-17	1.341000698527264E-5
18-19	2.2350011642121066E-6
20-21	5.25225273589845E-4
22-23	6.414453341288746E-4
24-25	0.002833981476220951
26-27	0.0023646312317364086
28-29	0.003424021783572947
30-31	0.0037033969290994604
32-33	0.0038553770082658837
34-35	0.004154867164270306
36-37	0.003222871678793858
38-39	0.001951156016357169
40-41	0.0032541616950928273
42-43	0.0051494426823446935
44-45	0.005019812614820391
46-47	0.01233050142295819
48-49	0.005039927625298301
50-51	0.006995553643983894
52-53	0.0025702513388439225
54-55	0.0012113706310029617
56-57	0.005583032908201842
58-59	0.0025791913435007706
60-61	0.014359882480062786
62-63	0.01247801149979619
64-65	0.009863060137668026
66-67	0.012019836261132708
68-69	0.009818360114383784
70-71	0.008669569515978762
72-73	0.005645612940799781
74-75	0.003801736980324793
76-77	5.87805306187784E-4
78-79	0.010158080291344023
80-81	0.006287058274928655
82-83	0.010580495511380112
84-85	0.00845724440537861
86-87	0.007543128929215859
88-89	0.009574744987484663
90-91	0.005448932838349116
92-93	0.009297604843122363
94-95	0.003140176635718009
96-97	0.005670197953606114
98-99	0.4488105837854331
100	1.7489554610308995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.2371353E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.90882583555629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.19669310587919	68.67770291941892
2	5.038462858140742	7.34696822006205
3	0.516641131346424	1.1300309479446358
4	0.09287101793378208	0.2708446748681977
5	0.03426809933807503	0.1249223443177627
6	0.018660158381889332	0.08162941425174391
7	0.014927626024268368	0.07618489801591902
8	0.011704822014473282	0.06827078637515341
9	0.008073461829913666	0.052976396020246834
>10	0.05088739760215051	0.7319626912302153
>50	0.006837150939103713	0.35087475871488705
>100	0.007426324498800061	1.0270044817655166
>500	6.920690738777015E-4	0.3579816903481291
>1k	8.846502097862912E-4	1.3905701833336437
>5k	2.39461675451018E-4	1.267979502071481
>10k+	7.306651122736049E-4	17.04409609126153
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97865	0.4374567778712356	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76864	0.34358225897199873	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76529	0.34208480819197656	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75475	0.3373734257378175	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68866	0.3078311803492619	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68748	0.3073037200745078	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63385	0.28333109758716873	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61885	0.2766260940945324	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61346	0.2742167628395118	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60850	0.2719996416846134	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59363	0.2653527482222466	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59057	0.2639849275097487	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57423	0.2566809437051036	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56570	0.2528680317189577	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56072	0.25064197055940246	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55343	0.24738333886198122	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54894	0.24537630781651873	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54702	0.2445180673694613	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50479	0.22564124753652587	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50238	0.2245639769753756	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49835	0.22276256603702063	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48586	0.2171795331288188	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46744	0.2089457888398614	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46676	0.20864182868152856	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45974	0.20550388704697475	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44964	0.2009891846952663	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44844	0.2004527844158554	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44560	0.19918330375458293	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44362	0.19829824329355492	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44339	0.1981954332400012	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42890	0.1917183998661145	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41280	0.1845216961173515	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41214	0.1842266759636755	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41126	0.18383331575877418	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41116	0.18378861573548994	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40306	0.18016791384946632	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40075	0.17913534331160033	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38597	0.17252867987018933	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38101	0.1703115587152909	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37942	0.16960082834507148	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37889	0.169363918221665	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37582	0.16799162750683877	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37520	0.16771448736247646	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37271	0.16660145678269883	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36601	0.1636065552226546	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36050	0.16114358393969289	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34834	0.15570806110832902	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34807	0.15558737104546158	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33747	0.1508491685773319	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32805	0.1466384263839563	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32238	0.14410393506373978	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32131	0.14362564481459839	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31692	0.14166331379242017	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31237	0.13962946273298715	No Hit
CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30957	0.13837786208102837	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30703	0.1372424814896086	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29602	0.13232100892601356	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28922	0.12928140734268506	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28581	0.12775713654869242	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28491	0.12735483633913425	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28423	0.1270508761808014	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28363	0.12678267604109594	No Hit
CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27980	0.12507066514930948	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26522	0.11855340175446698	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26518	0.11853552174515328	No Hit
GCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24830	0.11099015781477321	No Hit
CATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23946	0.10703867575644621	No Hit
ACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23581	0.10540712490657135	No Hit
CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23073	0.10313636372373186	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22424	0.10023533221258454	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0018505809639676241	0.0	0.0	4.470002328424213E-6	0.0
2	0.004756082477443363	0.0	0.0	4.470002328424213E-6	4.470002328424213E-6
3	0.00803259418417831	0.0	0.0	1.341000698527264E-5	8.940004656848426E-6
4	0.013311666934047306	0.0	0.0	1.341000698527264E-5	8.940004656848426E-6
5	0.022868531912218274	0.0	0.0	1.341000698527264E-5	8.940004656848426E-6
6	0.042617002199196445	0.0	0.0	1.341000698527264E-5	8.940004656848426E-6
7	0.06682206480761356	0.0	0.0	1.341000698527264E-5	8.940004656848426E-6
8	0.17369535047790807	4.470002328424213E-6	0.0	1.341000698527264E-5	8.940004656848426E-6
9	0.6058641155946178	4.470002328424213E-6	0.0	1.341000698527264E-5	8.940004656848426E-6
10-11	1.4616706463842397	4.470002328424213E-6	2.2350011642121066E-6	1.5645008149484746E-5	8.940004656848426E-6
12-13	2.39680854349757	4.470002328424213E-6	4.470002328424213E-6	1.7880009313696852E-5	8.940004656848426E-6
14-15	3.422260602655548	4.470002328424213E-6	4.470002328424213E-6	2.682001397054528E-5	8.940004656848426E-6
16-17	4.458440667401744	4.470002328424213E-6	4.470002328424213E-6	4.693502444845424E-5	8.940004656848426E-6
18-19	5.582590377971329	4.470002328424213E-6	4.470002328424213E-6	5.5875029105302664E-5	8.940004656848426E-6
20-21	7.040671612485843	4.470002328424213E-6	4.470002328424213E-6	6.034503143372688E-5	8.940004656848426E-6
22-23	8.72739570110042	4.470002328424213E-6	4.470002328424213E-6	6.70500349263632E-5	8.940004656848426E-6
24-25	10.581382806842303	4.470002328424213E-6	4.470002328424213E-6	6.70500349263632E-5	8.940004656848426E-6
26-27	12.485000348436682	4.470002328424213E-6	4.470002328424213E-6	6.70500349263632E-5	8.940004656848426E-6
28-29	14.50719364179717	4.470002328424213E-6	4.470002328424213E-6	7.152003725478741E-5	8.940004656848426E-6
30-31	16.579359326188275	4.470002328424213E-6	4.470002328424213E-6	7.152003725478741E-5	8.940004656848426E-6
32-33	18.90629502828908	4.470002328424213E-6	4.470002328424213E-6	7.152003725478741E-5	8.940004656848426E-6
34-35	21.118005245368934	4.470002328424213E-6	4.470002328424213E-6	7.152003725478741E-5	8.940004656848426E-6
36-37	22.819723956794206	4.470002328424213E-6	4.470002328424213E-6	7.822504074742374E-5	8.940004656848426E-6
38-39	24.09421549067685	4.470002328424213E-6	4.470002328424213E-6	1.028100535537569E-4	8.940004656848426E-6
40-41	25.28655732176771	4.470002328424213E-6	4.470002328424213E-6	1.0951505704639321E-4	8.940004656848426E-6
42-43	26.261315084519026	4.470002328424213E-6	4.470002328424213E-6	1.318650686885143E-4	8.940004656848426E-6
44-45	27.040961715636957	4.470002328424213E-6	4.470002328424213E-6	1.6539008615169587E-4	8.940004656848426E-6
46-47	27.693439015512382	4.470002328424213E-6	4.470002328424213E-6	1.7880009313696852E-4	8.940004656848426E-6
48-49	28.272467472128305	4.470002328424213E-6	4.470002328424213E-6	2.10090109435938E-4	8.940004656848426E-6
50-51	28.83747353143996	4.470002328424213E-6	4.470002328424213E-6	2.6596513854124066E-4	8.940004656848426E-6
52-53	29.43869331461535	4.470002328424213E-6	4.470002328424213E-6	3.441901792886644E-4	8.940004656848426E-6
54-55	30.018604596691134	4.470002328424213E-6	8.940004656848426E-6	3.844202002444823E-4	8.940004656848426E-6
56-57	30.60056313983334	4.470002328424213E-6	1.1175005821060533E-5	3.866552014086944E-4	8.940004656848426E-6
58-59	31.155426763861804	4.470002328424213E-6	1.341000698527264E-5	3.9336020490133075E-4	8.940004656848426E-6
60-61	31.69458279970818	4.470002328424213E-6	1.341000698527264E-5	4.1124021421502755E-4	8.940004656848426E-6
62-63	32.23073946399219	4.470002328424213E-6	1.341000698527264E-5	4.2912022352872446E-4	8.940004656848426E-6
64-65	32.75099632999399	4.470002328424213E-6	1.341000698527264E-5	4.492352340066334E-4	8.940004656848426E-6
66-67	33.227402473153944	4.470002328424213E-6	1.341000698527264E-5	5.319302770824813E-4	8.940004656848426E-6
68-69	33.65545660112734	4.470002328424213E-6	1.341000698527264E-5	5.922753085162082E-4	8.940004656848426E-6
70-71	34.05944200156334	4.470002328424213E-6	1.341000698527264E-5	6.302703283078139E-4	8.940004656848426E-6
72-73	34.45634468330995	4.470002328424213E-6	1.341000698527264E-5	6.503853387857231E-4	8.940004656848426E-6
74-75	34.83759565190357	4.470002328424213E-6	1.341000698527264E-5	6.70500349263632E-4	8.940004656848426E-6
76-77	35.210932481374726	4.470002328424213E-6	1.341000698527264E-5	7.531953923394798E-4	8.940004656848426E-6
78-79	35.561172808814916	4.470002328424213E-6	1.341000698527264E-5	9.051754715059031E-4	8.940004656848426E-6
80-81	35.89953410506732	4.470002328424213E-6	1.341000698527264E-5	0.0010102205262238722	8.940004656848426E-6
82-83	36.237817176278966	4.470002328424213E-6	1.341000698527264E-5	0.001032570537865993	8.940004656848426E-6
84-85	36.576759572834064	4.470002328424213E-6	1.341000698527264E-5	0.00105045054717969	8.940004656848426E-6
86-87	36.91594558451605	4.470002328424213E-6	1.341000698527264E-5	0.0010549205495081141	8.940004656848426E-6
88	37.169450591566815	4.470002328424213E-6	1.341000698527264E-5	0.0010593905518365384	8.940004656848426E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	5035	0.0	65.88803	1
TGCCCTA	14635	0.0	53.02507	6
GACTTTT	4555	0.0	51.96327	1
CACTTTT	7550	0.0	48.988308	1
GCCCTAC	37550	0.0	48.775356	7
TGCTCTA	13835	0.0	48.723297	6
GCTTTCT	5305	0.0	48.51978	1
CCTTTTC	9905	0.0	46.794907	1
GCATTTT	4050	0.0	46.47526	1
CCCCTAC	94580	0.0	46.09984	7
CCCCCTA	50795	0.0	45.175976	6
ATTTCTT	27550	0.0	44.946064	2
GCCCCTA	10900	0.0	44.862984	6
CCCTTTT	10360	0.0	44.694305	1
TTGCCCT	6200	0.0	44.49332	5
ATGCCCT	5140	0.0	44.434628	5
GATTTCT	5620	0.0	44.041924	1
ACTTTTC	9360	0.0	43.68786	1
GCTTTTC	6495	0.0	43.68728	1
CCGCCTA	7135	0.0	42.99155	6
>>END_MODULE