##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248485_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19387036
Sequences flagged as poor quality	0
Sequence length	100
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.620174378383574	31.0	30.0	33.0	23.0	34.0
2	30.119364919939283	31.0	30.0	34.0	25.0	34.0
3	30.434061555361016	31.0	30.0	34.0	26.0	34.0
4	33.87011856789248	35.0	35.0	37.0	28.0	37.0
5	33.657096164674165	35.0	35.0	37.0	28.0	37.0
6	33.415540673674926	35.0	33.0	37.0	27.0	37.0
7	33.50106277205035	35.0	33.0	37.0	28.0	37.0
8	33.50356655860133	35.0	33.0	37.0	28.0	37.0
9	34.767288305442875	37.0	34.0	39.0	27.0	39.0
10-11	35.297237932606095	37.0	35.0	39.0	29.5	39.0
12-13	35.590911421426156	39.0	35.0	39.0	30.0	39.0
14-15	36.40735345000649	39.0	36.0	40.0	30.0	41.0
16-17	36.10142210495715	38.5	35.0	40.0	30.0	41.0
18-19	35.636868756007885	38.0	34.0	40.0	28.0	41.0
20-21	35.20759570983414	37.5	34.0	40.0	27.0	41.0
22-23	34.87589497951105	37.0	34.0	39.0	27.0	41.0
24-25	34.58270356541351	37.0	33.0	39.0	26.0	41.0
26-27	34.30163140977301	36.5	33.0	39.0	25.0	40.5
28-29	34.14582680405607	36.0	33.0	39.0	25.0	40.0
30-31	34.00877991354635	36.0	33.0	39.0	25.0	40.0
32-33	33.85296197417697	36.0	32.5	39.0	25.0	40.0
34-35	33.66393060290392	35.0	32.0	39.0	24.5	40.0
36-37	33.37942385829376	35.0	31.0	39.0	24.0	40.0
38-39	33.118934477658165	35.0	30.5	38.5	23.5	40.0
40-41	32.83425808359772	35.0	30.0	38.0	23.0	40.0
42-43	32.5591782054771	35.0	30.0	38.0	22.0	40.0
44-45	32.178695082631506	35.0	29.5	38.0	22.0	40.0
46-47	32.05059545977012	35.0	28.5	38.0	22.0	40.0
48-49	32.0948092323138	35.0	29.0	38.0	22.0	39.5
50-51	31.954320015705342	35.0	29.0	37.0	22.0	39.0
52-53	31.84481137291951	34.5	29.0	37.0	22.0	39.0
54-55	31.625587531791865	34.0	29.0	36.5	21.5	39.0
56-57	31.91849282685605	34.0	29.5	36.0	22.5	39.0
58-59	32.062537873246846	34.0	30.0	36.0	23.5	39.0
60-61	31.88119248863003	34.0	30.0	35.5	23.0	39.0
62-63	31.637929877470697	34.0	30.0	35.0	23.0	38.5
64-65	31.427608093367134	34.0	30.0	35.0	23.0	37.5
66-67	31.232913298350503	34.0	29.0	35.0	23.0	37.0
68-69	31.081856066084573	34.0	29.0	35.0	22.5	36.5
70-71	30.95839547107665	34.0	29.0	35.0	23.0	36.0
72-73	30.867249924124557	34.0	29.0	35.0	22.5	36.0
74-75	30.77967261215175	33.5	29.0	35.0	22.0	35.5
76-77	30.651558881925013	33.0	29.0	35.0	20.5	35.0
78-79	30.528309948978276	33.0	29.0	35.0	20.0	35.0
80-81	30.44045319769355	33.0	29.0	35.0	20.0	35.0
82-83	30.334777838138848	34.0	29.0	35.0	20.0	35.0
84-85	30.228804057515546	34.0	29.0	35.0	19.5	35.0
86-87	30.057581081502093	34.0	29.0	35.0	18.5	35.0
88-89	29.86762726390976	34.0	29.0	35.0	17.0	35.0
90-91	29.604989592013965	33.0	28.0	35.0	15.0	35.0
92-93	29.32867321750473	33.0	28.0	35.0	11.0	35.0
94-95	29.07580359370045	33.0	27.0	35.0	10.0	35.0
96-97	28.772840520851148	33.0	27.0	35.0	10.0	35.0
98-99	28.41631920939333	33.0	25.5	35.0	9.0	35.0
100	26.669026766133822	31.0	23.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33943758793278533
1101	2	0.37057572625740676
1101	3	0.4466792916208959
1101	4	0.33958884147151736
1101	5	0.26209513328957
1101	6	0.18119392514930865
1101	7	0.30696930354711327
1101	8	0.3174473325253828
1101	9	0.3899388680053093
1101	10-11	0.5180347045032008
1101	12-13	0.5714037463182251
1101	14-15	0.7606621005144518
1101	16-17	0.793453527731387
1101	18-19	0.7595611764122907
1101	20-21	0.6664907169612491
1101	22-23	0.6662483230563723
1101	24-25	0.7454940755416075
1101	26-27	0.7653038421603426
1101	28-29	0.8010641307669459
1101	30-31	0.7698145577067876
1101	32-33	0.786642545982069
1101	34-35	0.8471008635297679
1101	36-37	0.8860414314622815
1101	38-39	0.8088949170147544
1101	40-41	0.7916430077622607
1101	42-43	0.7949529304904104
1101	44-45	0.7199886632057257
1101	46-47	0.7689496974978525
1101	48-49	0.8141636265346648
1101	50-51	0.7740881839018172
1101	52-53	0.7573219561918734
1101	54-55	0.7176475232666988
1101	56-57	0.6679532861724411
1101	58-59	0.6378568122062447
1101	60-61	0.6460239479609022
1101	62-63	0.6195292838857789
1101	64-65	0.6329874206066606
1101	66-67	0.615544124911267
1101	68-69	0.5839187105916643
1101	70-71	0.6253766662727074
1101	72-73	0.6366624407008921
1101	74-75	0.6080229268821924
1101	76-77	0.6162741484719163
1101	78-79	0.5304280956256306
1101	80-81	0.5893461411538574
1101	82-83	0.5645962289619426
1101	84-85	0.5447047168772343
1101	86-87	0.5336259874687599
1101	88-89	0.5060500287901171
1101	90-91	0.5179947584856954
1101	92-93	0.4656789612605863
1101	94-95	0.4430771248791956
1101	96-97	0.42985621306086585
1101	98-99	0.4157298279796038
1101	100	0.41405421101708484
1102	1	0.28172446656445516
1102	2	0.3210822144009633
1102	3	0.32510945873114494
1102	4	0.2636903944348248
1102	5	0.1984567988040311
1102	6	0.08296441209745353
1102	7	0.13637720230482842
1102	8	0.17416314608037453
1102	9	0.1373506845853072
1102	10-11	0.29130892365471084
1102	12-13	0.4123856311198679
1102	14-15	0.4864005155176443
1102	16-17	0.5162086359182467
1102	18-19	0.47489812823530286
1102	20-21	0.4133722748216684
1102	22-23	0.4388942479097864
1102	24-25	0.42132501123910515
1102	26-27	0.38762892260235304
1102	28-29	0.4260661169767843
1102	30-31	0.4049238304491425
1102	32-33	0.39709319128417064
1102	34-35	0.4633933192030142
1102	36-37	0.4513155199206409
1102	38-39	0.4483346692209764
1102	40-41	0.38815898219797873
1102	42-43	0.43541670839044855
1102	44-45	0.36076624497868437
1102	46-47	0.3855696142495688
1102	48-49	0.4137953369628704
1102	50-51	0.42520791278173675
1102	52-53	0.4649516911531322
1102	54-55	0.4804436595340533
1102	56-57	0.46136771550956013
1102	58-59	0.4417032672966883
1102	60-61	0.4714877909184416
1102	62-63	0.46983339134699165
1102	64-65	0.46317891272720857
1102	66-67	0.4805490020874288
1102	68-69	0.47721298244229615
1102	70-71	0.5011638443967001
1102	72-73	0.4219016381961431
1102	74-75	0.39438625630397794
1102	76-77	0.42666654397772774
1102	78-79	0.45042212736552756
1102	80-81	0.40383508718637984
1102	82-83	0.42466640490945196
1102	84-85	0.38383666382348025
1102	86-87	0.3833329052199623
1102	88-89	0.39536962649486895
1102	90-91	0.35623704917068366
1102	92-93	0.33848412790540294
1102	94-95	0.3161431263972716
1102	96-97	0.30189941666681364
1102	98-99	0.29166671063145344
1102	100	0.25537335479925005
1103	1	0.2921979650006321
1103	2	0.28439679452514355
1103	3	0.22603469538575993
1103	4	0.244073346725024
1103	5	0.17313640452833567
1103	6	0.16393608139799198
1103	7	0.18273938226898423
1103	8	0.2650132247831749
1103	9	0.1863015112630393
1103	10-11	0.28610927150158005
1103	12-13	0.40916522938635325
1103	14-15	0.4234678213150005
1103	16-17	0.4214827279015765
1103	18-19	0.36646458437311935
1103	20-21	0.36865701430780007
1103	22-23	0.32412477120033856
1103	24-25	0.3097989349868868
1103	26-27	0.33012193952805546
1103	28-29	0.38493228313382133
1103	30-31	0.3104737129109836
1103	32-33	0.3420128862056302
1103	34-35	0.36242819965415407
1103	36-37	0.3307835814508806
1103	38-39	0.30275770028887194
1103	40-41	0.2680609923632318
1103	42-43	0.35770971469071355
1103	44-45	0.29422591730071446
1103	46-47	0.3015138620005544
1103	48-49	0.37532705770098573
1103	50-51	0.43901772610704626
1103	52-53	0.41893688284106645
1103	54-55	0.35355429900523916
1103	56-57	0.3583648137009092
1103	58-59	0.4132393820975153
1103	60-61	0.395592869191713
1103	62-63	0.4183942607752549
1103	64-65	0.37235314805385045
1103	66-67	0.4114939378727769
1103	68-69	0.44220040854628095
1103	70-71	0.4063448247993975
1103	72-73	0.430698958250435
1103	74-75	0.4491136835428904
1103	76-77	0.43426374687271263
1103	78-79	0.4363952900677148
1103	80-81	0.46664940460987125
1103	82-83	0.48882903869527894
1103	84-85	0.45728734439782315
1103	86-87	0.4603936468472938
1103	88-89	0.43693484603933186
1103	90-91	0.4250939082891847
1103	92-93	0.37167944511102036
1103	94-95	0.39003023614963084
1103	96-97	0.4094148160793303
1103	98-99	0.3752830433681389
1103	100	0.32834250400934195
1104	1	0.14319632372012947
1104	2	0.15217770053601853
1104	3	0.20115848216891052
1104	4	0.12157085986835625
1104	5	0.09369574817086601
1104	6	0.09000194495322233
1104	7	0.0973615952229352
1104	8	0.13350383730939086
1104	9	0.04584927600699018
1104	10-11	0.11689128318885622
1104	12-13	0.2231709001310307
1104	14-15	0.2760948337071838
1104	16-17	0.24968844518867428
1104	18-19	0.23739688916791124
1104	20-21	0.21124694471586736
1104	22-23	0.23425396755726524
1104	24-25	0.2565991736532851
1104	26-27	0.26661888978284054
1104	28-29	0.19265202086326383
1104	30-31	0.25102504252364355
1104	32-33	0.254716508496017
1104	34-35	0.23793404470846014
1104	36-37	0.2275157333284099
1104	38-39	0.20150022564492787
1104	40-41	0.14773700299822679
1104	42-43	0.2052758278468545
1104	44-45	0.225498862572735
1104	46-47	0.23664522902537755
1104	48-49	0.23768129743845634
1104	50-51	0.25419401715019063
1104	52-53	0.2633457569596658
1104	54-55	0.2619368588958153
1104	56-57	0.2917272090020262
1104	58-59	0.23931523383340902
1104	60-61	0.3089278156461539
1104	62-63	0.3040366309967695
1104	64-65	0.27592493112576477
1104	66-67	0.2628501166020847
1104	68-69	0.31014358104803463
1104	70-71	0.27652779846037134
1104	72-73	0.3064170106988868
1104	74-75	0.31178702354836574
1104	76-77	0.2932307051284049
1104	78-79	0.3283733684287924
1104	80-81	0.28804380090896586
1104	82-83	0.34348058139602955
1104	84-85	0.3338635756638517
1104	86-87	0.31990371760657865
1104	88-89	0.3979102954919185
1104	90-91	0.36150654614213806
1104	92-93	0.3471184916169179
1104	94-95	0.35983214966676513
1104	96-97	0.34457728737626425
1104	98-99	0.3637347586309403
1104	100	0.4414497047484929
1105	1	0.054073490480227804
1105	2	0.15813264230141044
1105	3	0.1549605851096274
1105	4	0.15541111228503013
1105	5	0.06373828200797504
1105	6	0.07587900549413007
1105	7	0.09421340649099363
1105	8	0.12145404998644693
1105	9	0.11691430596788166
1105	10-11	0.1962291400086329
1105	12-13	0.2225958759668103
1105	14-15	0.279914990632534
1105	16-17	0.28110834574023613
1105	18-19	0.22666774082186691
1105	20-21	0.19047685988581264
1105	22-23	0.18460435517339135
1105	24-25	0.20498383870167913
1105	26-27	0.19739928718529853
1105	28-29	0.17480528036840326
1105	30-31	0.1759193714729932
1105	32-33	0.1591638202613126
1105	34-35	0.2076794795255168
1105	36-37	0.23761482023150649
1105	38-39	0.22566773402221685
1105	40-41	0.21181504911162108
1105	42-43	0.22566223845690558
1105	44-45	0.17135773419128242
1105	46-47	0.19211554768452999
1105	48-49	0.22575461750584225
1105	50-51	0.23904482062102517
1105	52-53	0.2789342006732767
1105	54-55	0.2686119117208534
1105	56-57	0.3002560051042309
1105	58-59	0.2710756423046945
1105	60-61	0.2762227093983718
1105	62-63	0.2640049981339452
1105	64-65	0.292450403852321
1105	66-67	0.2804423565634657
1105	68-69	0.3086611794178893
1105	70-71	0.3353140954604612
1105	72-73	0.33583877674798757
1105	74-75	0.331199968683503
1105	76-77	0.2922373989571092
1105	78-79	0.3241558120587875
1105	80-81	0.31725821266628174
1105	82-83	0.35929720164184786
1105	84-85	0.32797333318982425
1105	86-87	0.3098279549960381
1105	88-89	0.33191480933558637
1105	90-91	0.31632438389596373
1105	92-93	0.2913409857369622
1105	94-95	0.29887554144024264
1105	96-97	0.22244674514065466
1105	98-99	0.24386619937602916
1105	100	0.2904950931805459
1106	1	-0.023417156100666858
1106	2	0.011121643034098128
1106	3	0.06873591529769385
1106	4	0.06457546099273515
1106	5	-0.021815605077279088
1106	6	-0.035756977959223946
1106	7	0.022758117173545145
1106	8	0.10804544112658476
1106	9	0.05076566976651975
1106	10-11	0.12108023537756907
1106	12-13	0.19247667222674636
1106	14-15	0.2095439987148353
1106	16-17	0.16482124141469967
1106	18-19	0.07338923672116948
1106	20-21	0.1288783792808914
1106	22-23	0.13213035659975958
1106	24-25	0.08626038630343658
1106	26-27	0.0612678229429946
1106	28-29	0.1327455442708967
1106	30-31	0.1010957124602001
1106	32-33	0.08415875628578107
1106	34-35	0.18898210369234647
1106	36-37	0.19625647572613758
1106	38-39	0.14555872234213751
1106	40-41	0.14298077128023579
1106	42-43	0.13714123362099429
1106	44-45	0.1695420364088065
1106	46-47	0.22253473521664802
1106	48-49	0.22049413355581038
1106	50-51	0.24100471178444138
1106	52-53	0.24181245151537212
1106	54-55	0.21774016537718666
1106	56-57	0.17068057316870977
1106	58-59	0.22108389847652177
1106	60-61	0.2968254724352697
1106	62-63	0.2790982272270135
1106	64-65	0.2803042496312216
1106	66-67	0.2538314465973883
1106	68-69	0.26091012392471313
1106	70-71	0.25072310703554024
1106	72-73	0.24420894058510711
1106	74-75	0.2072584565546407
1106	76-77	0.21984658915787847
1106	78-79	0.20093100636618288
1106	80-81	0.19588023040531866
1106	82-83	0.20705167686759296
1106	84-85	0.17707703160649402
1106	86-87	0.23914408808834509
1106	88-89	0.2339140251519609
1106	90-91	0.22083121030206243
1106	92-93	0.20087256258688058
1106	94-95	0.22461932734270107
1106	96-97	0.2150384330193873
1106	98-99	0.1804051873863699
1106	100	0.2270792786269027
1107	1	0.06328945450627899
1107	2	0.06703338145709381
1107	3	0.074857046079007
1107	4	0.04091104041746263
1107	5	0.03148412691398761
1107	6	-0.012993037455828471
1107	7	0.06401480710047736
1107	8	0.08253803287350081
1107	9	0.003223756215085416
1107	10-11	0.11982574277233482
1107	12-13	0.16676465480001212
1107	14-15	0.21886739914518927
1107	16-17	0.17682024120980344
1107	18-19	0.14967871420722645
1107	20-21	0.18277324906482306
1107	22-23	0.1603857004558833
1107	24-25	0.1772473288845262
1107	26-27	0.17142282081251636
1107	28-29	0.21275151831078887
1107	30-31	0.19172698152348033
1107	32-33	0.26433718422851626
1107	34-35	0.28229916756929896
1107	36-37	0.2979047885977977
1107	38-39	0.27307692044522724
1107	40-41	0.3119923453328681
1107	42-43	0.3115895130060409
1107	44-45	0.2559292655211607
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2216	22-23	0.07735706974013823
2216	24-25	0.19619160110151768
2216	26-27	0.22951256263626618
2216	28-29	0.25577093088890024
2216	30-31	0.22351593777994339
2216	32-33	0.26745352371282394
2216	34-35	0.24680142020301332
2216	36-37	0.34992174498437834
2216	38-39	0.29483785991612166
2216	40-41	0.31601331809289235
2216	42-43	0.406449356390425
2216	44-45	0.4350169592902162
2216	46-47	0.3293195625388421
2216	48-49	0.3014233546634557
2216	50-51	0.29649242395829134
2216	52-53	0.36402242435215726
2216	54-55	0.382599189400473
2216	56-57	0.3563089862480098
2216	58-59	0.264159565541334
2216	60-61	0.2646953708677664
2216	62-63	0.26523767480488303
2216	64-65	0.22759922688864265
2216	66-67	0.21047955877277502
2216	68-69	0.1943012856057429
2216	70-71	0.18002785698237034
2216	72-73	0.09607827605568886
2216	74-75	0.11608201188388989
2216	76-77	0.03514193949477473
2216	78-79	0.03422084711449003
2216	80-81	-0.012306279902652761
2216	82-83	-0.14153846681383442
2216	84-85	-0.1294531287805718
2216	86-87	-0.149429377905534
2216	88-89	-0.23859323966261314
2216	90-91	-0.26581372753757293
2216	92-93	-0.14640803362328825
2216	94-95	-0.14794438184913616
2216	96-97	-0.1308503434883157
2216	98-99	-0.05660317052404906
2216	100	0.3096821558195124
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	508.0
6	3812.0
7	11825.0
8	24616.0
9	41435.0
10	57792.0
11	67861.0
12	70477.0
13	72329.0
14	77542.0
15	84784.0
16	93476.0
17	105100.0
18	119038.0
19	133501.0
20	151538.0
21	172630.0
22	193141.0
23	222936.0
24	300606.0
25	363269.0
26	331325.0
27	582624.0
28	467648.0
29	721607.0
30	733782.0
31	908809.0
32	1193644.0
33	1880576.0
34	2425531.0
35	4556934.0
36	2292520.0
37	796569.0
38	124093.0
39	3150.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.113875612352974	29.307637271505627	26.439338048762696	28.1391490673787
2	14.086631964638077	30.471587724838688	28.968107678591092	26.473672631932143
3	12.587942788160088	32.653717669890334	26.52225435595209	28.23608518599749
4	11.460499246016733	31.815705702290213	26.6809081429935	30.04288690869955
5	11.430937663704755	31.543460279333058	27.546191176412936	29.47941088054925
6	11.14695143414709	28.350423412497154	28.784049309883642	31.71857584347211
7	15.336930698140591	32.57425645029917	24.207811958640878	27.88100089291936
8	13.56940276997474	30.136736734795356	21.559092374925182	34.73476812030472
9	10.749626709312347	28.88857275552591	23.108911542744337	37.252888992417404
10-11	15.553193833005292	43.35452049144634	21.81733363879608	19.274952036752296
12-13	15.023510563505601	44.79615495514269	20.88046492938836	19.29986955196335
14-15	16.42443372285736	43.67758128450468	20.271603815586996	19.626381177050966
16-17	16.568713967949698	43.31097805340472	20.791475480713398	19.328832497932183
18-19	17.19584213143235	41.39581829587305	21.924366278321394	19.483973294373204
20-21	15.95822998776217	40.940696992680955	23.208140540021667	19.89293247953521
22-23	16.11206464117109	41.757959253676106	23.92755206123683	18.20242404391597
24-25	16.133948995607167	41.82158118445749	23.230495368141888	18.813974451793456
26-27	17.88462145528589	42.123870817591715	21.85010385290459	18.1414038742178
28-29	17.195152987800714	43.045187516028754	22.786760699263155	16.972898796907376
30-31	17.444772029265376	42.600897219508774	22.762839192395155	17.191491558830695
32-33	18.024369646146116	42.834490743874994	22.089523522881475	17.051616087097415
34-35	18.10818571648011	42.90141070718781	21.910380319527288	17.08002325680479
36-37	18.6236631186715	42.63370489665629	21.622545617541235	17.120086367130973
38-39	19.009300753915408	42.76009142863981	21.532782690682787	16.69782512676199
40-41	19.32297833370971	42.98914936864468	21.4677986038474	16.220073693798216
42-43	19.53504562659996	43.28118005076333	21.614230631964684	15.569543690672022
44-45	19.770101525515955	43.01656748568328	21.51517497673306	15.698156012067708
46-47	19.900996168732572	42.62517320628414	21.772803956315613	15.701026668667673
48-49	19.751886516711618	42.73888267949811	22.107714791999907	15.401516011790362
50-51	19.902003070505465	42.55751884919386	22.440653125108962	15.099824955191707
52-53	19.9571218246601	42.58490952115949	22.67869031334833	14.77927834083208
54-55	19.92692809775162	42.716658313961176	22.71647468511263	14.63993890317457
56-57	19.800210760548474	42.885384638000964	22.331374797350588	14.983029804099978
58-59	19.62320515470658	42.82792915902031	22.426442436707077	15.122423249566042
60-61	19.575713148982036	42.58060371355059	22.694236926649733	15.14944621081764
62-63	19.711035125457567	42.03697035429889	22.884356021586225	15.367638498657321
64-65	19.597408160775736	41.90059269082563	22.90682341083628	15.595175737562354
66-67	19.303905266012762	41.67584549689529	23.22344888601136	15.796800351080584
68-69	19.10376610323972	41.735117551618956	23.00679346459473	16.154322880546594
70-71	18.73036433437892	41.86607606903542	23.129895956633845	16.27366363995181
72-73	18.648703631375458	42.28281650044122	23.102906887136918	15.9655729810464
74-75	18.464426920131746	42.769407938181985	22.95616790668764	15.80999723499863
76-77	17.95827485762601	43.47324708020357	22.9757716626963	15.592706399474121
78-79	17.528955467698324	44.20840483249434	22.744105453777834	15.5185342460295
80-81	17.083447446450826	45.1893232367199	22.356730771437856	15.37049854539142
82-83	16.64322048734422	46.0414255896001	22.240817201093066	15.074536721962614
84-85	16.222875492807102	47.023969180006944	22.029080880198666	14.724074446987284
86-87	15.869395301161497	47.74594206952559	21.73507209109543	14.649590538217492
88-89	15.61119679369468	48.32584799966988	21.568781335754224	14.49417387088122
90-91	15.516539515803862	48.74558030760394	21.30561634859737	14.432263827994834
92-93	15.55410067758427	48.94229669409285	20.96555210319346	14.538050525129421
94-95	15.633625264930767	49.058873736134615	20.64422146403001	14.663279534904605
96-97	15.78920722657804	48.81818309598888	20.505720161918553	14.886889515514529
98-99	16.031674980926624	48.385070222153814	20.367307985625192	15.215946811294367
100	16.507545112007847	47.407341345084525	20.18450026141423	15.900613281493401
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1217645.0
1	1198319.5
2	748343.5
3	200679.5
4	82039.5
5	78882.0
6	69344.5
7	51940.5
8	36799.5
9	29753.5
10	30078.5
11	35231.0
12	44509.5
13	58361.0
14	77587.5
15	101695.0
16	129605.5
17	160627.5
18	194310.5
19	227707.0
20	258195.5
21	286666.5
22	311111.5
23	330947.0
24	345819.5
25	353702.5
26	355618.5
27	351074.0
28	339529.5
29	322861.5
30	303265.0
31	281522.5
32	258218.0
33	236472.5
34	216396.0
35	197305.0
36	180325.0
37	165980.5
38	155299.5
39	149131.0
40	148002.0
41	154134.5
42	171083.5
43	201180.0
44	246543.0
45	306183.0
46	372771.5
47	439396.0
48	502434.5
49	562137.0
50	624155.5
51	694917.5
52	766773.5
53	816861.0
54	820402.5
55	761764.0
56	645164.0
57	494378.5
58	344719.0
59	221426.5
60	133842.5
61	79554.5
62	49096.0
63	33345.5
64	24830.5
65	19927.5
66	16695.5
67	14267.0
68	12386.5
69	10847.5
70	9466.0
71	8363.5
72	7645.5
73	6954.5
74	6373.0
75	5983.5
76	5609.5
77	5337.5
78	5021.5
79	4658.5
80	4418.0
81	4262.5
82	4124.5
83	3985.5
84	3826.5
85	3696.0
86	3669.5
87	3640.0
88	3532.5
89	3500.0
90	3419.5
91	3270.5
92	3170.5
93	3107.0
94	2928.0
95	2773.0
96	2680.0
97	2467.0
98	2185.0
99	1807.5
100	1329.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013343968619029745
2	4.17804970290456E-4
3	0.0
4	5.158086052968592E-6
5	0.0
6	0.003816983679196758
7	3.4559176554889567E-4
8	0.0
9	0.0
10-11	2.9143186199272544E-4
12-13	3.0948516317811547E-5
14-15	4.3585827147584605E-4
16-17	2.1921865725116514E-4
18-19	1.8311205488038502E-4
20-21	9.026650592695036E-5
22-23	5.673894658265451E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	1.5474258158905776E-4
32-33	4.384373145023304E-5
34-35	1.1347789316530902E-4
36-37	1.5990066764202636E-4
38-39	5.828637239854509E-4
40-41	0.0017021683974796356
42-43	0.0010780399850704356
44-45	4.4875348660826755E-4
46-47	5.648104228000608E-4
48-49	9.361926186137995E-4
50-51	0.0
52-53	8.768746290046608E-5
54-55	5.364409495087336E-4
56-57	5.467571216146707E-4
58-59	4.8228104595256335E-4
60-61	5.415990355617022E-5
62-63	2.2179770027764946E-4
64-65	1.5990066764202636E-4
66-67	1.7537492580093215E-4
68-69	1.0831980711234044E-4
70-71	6.18970326356231E-4
72-73	5.777056379324823E-4
74-75	3.3785463646944275E-4
76-77	3.507498516018643E-4
78-79	7.917662091306789E-4
80-81	0.0010599866838850458
82-83	4.229630563434245E-4
84-85	7.221320474156029E-4
86-87	0.001263731082977305
88-89	0.0012173083085005878
90-91	0.0011502531898119962
92-93	7.659757788658359E-4
94-95	6.782883159653698E-4
96-97	6.628140578064641E-4
98-99	3.249594213370213E-4
100	9.284554895343467E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9387036E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.46771029128574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.14498683651809	78.32711933556938
2	3.427654987294652	5.584864069668029
3	0.2475298872609652	0.6049707943143281
4	0.053620242366777975	0.17473273483540777
5	0.029539267010980978	0.12032482235337662
6	0.019322341734870744	0.09444881631634018
7	0.013073848739994625	0.07455675650793814
8	0.010798826967637834	0.07038045655081943
9	0.007100334225169582	0.05206031744646747
>10	0.04006263271272595	0.5971127806462619
>50	0.0025820660067058365	0.144449170582068
>100	0.0022587072703362625	0.37392730056606116
>500	4.8229236088595295E-4	0.29360511040166715
>1k	5.382544368382048E-4	0.9910620240495805
>5k	1.456046076950123E-4	0.8504281867930351
>10k+	3.0387048562437267E-4	11.645957323399285
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	570313	2.9417235311266765	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	149118	0.7691634760465705	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	132529	0.6835959865138745	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	121191	0.6251136068453167	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91880	0.4739249465467542	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68122	0.3513791381003264	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65609	0.3384168678492163	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64935	0.3349403178495155	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63569	0.3278943723011604	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53319	0.2750239902582323	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49236	0.25396352490396157	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48876	0.2521066139248929	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46854	0.2416769639257904	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44835	0.23126278818484683	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43654	0.22517108855629092	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41080	0.21189417505594974	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37385	0.1928350470902308	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33126	0.17086675859063757	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32904	0.16972166348687853	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32290	0.16655459865035582	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30388	0.1567439189776096	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27942	0.1441272404920484	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27660	0.14267266022511127	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24284	0.12525896171028927	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22906	0.11815111912929857	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21599	0.11140950065806861	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21222	0.10946490221609946	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21024	0.10844360117761168	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20573	0.10611730436772283	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0020426020769755623	0.0	0.0	0.0	0.0
2	0.005467571216146707	0.0	0.0	1.0316172105937184E-5	0.0
3	0.009511510681674084	0.0	0.0	4.1264688423748735E-5	5.158086052968592E-6
4	0.015453625814693901	0.0	0.0	4.1264688423748735E-5	5.158086052968592E-6
5	0.02538809955271141	5.158086052968592E-6	0.0	5.158086052968592E-5	5.158086052968592E-6
6	0.04324539346808867	5.158086052968592E-6	0.0	5.673894658265451E-5	1.0316172105937184E-5
7	0.06499188426740427	5.158086052968592E-6	0.0	6.18970326356231E-5	1.0316172105937184E-5
8	0.14450893885996807	5.158086052968592E-6	0.0	6.70551186885917E-5	1.0316172105937184E-5
9	0.438318678523112	5.158086052968592E-6	0.0	6.70551186885917E-5	1.0316172105937184E-5
10-11	1.0902285424136005	5.158086052968592E-6	0.0	6.963416171507599E-5	1.0316172105937184E-5
12-13	1.9565652016120463	5.158086052968592E-6	0.0	7.221320474156029E-5	1.0316172105937184E-5
14-15	2.9271261475967756	5.158086052968592E-6	0.0	7.737129079452888E-5	1.0316172105937184E-5
16-17	3.9546891025528605	5.158086052968592E-6	0.0	8.510841987398177E-5	1.0316172105937184E-5
18-19	5.057021093889752	5.158086052968592E-6	0.0	1.1863597921827761E-4	1.0316172105937184E-5
20-21	6.454498769177507	5.158086052968592E-6	2.579043026484296E-6	1.289521513242148E-4	1.0316172105937184E-5
22-23	8.01993662156505	5.158086052968592E-6	7.737129079452888E-6	1.289521513242148E-4	1.0316172105937184E-5
24-25	9.651980839154577	7.737129079452888E-6	1.0316172105937184E-5	1.315311943506991E-4	1.0316172105937184E-5
26-27	11.285059252997724	1.0316172105937184E-5	1.0316172105937184E-5	1.3668928040366768E-4	1.0316172105937184E-5
28-29	12.971098315389728	1.0316172105937184E-5	1.0316172105937184E-5	1.4184736645663626E-4	1.0316172105937184E-5
30-31	14.739638385155935	1.0316172105937184E-5	1.0316172105937184E-5	1.4442640948312058E-4	1.0316172105937184E-5
32-33	16.703399116811873	1.0316172105937184E-5	1.0316172105937184E-5	1.5216353856257347E-4	1.0316172105937184E-5
34-35	18.543659794101586	1.0316172105937184E-5	1.0316172105937184E-5	1.5732162461554205E-4	1.0316172105937184E-5
36-37	20.079678502686022	1.0316172105937184E-5	1.0316172105937184E-5	1.6247971066851065E-4	1.0316172105937184E-5
38-39	21.307370038411236	1.0316172105937184E-5	1.0316172105937184E-5	1.856910979068693E-4	1.0316172105937184E-5
40-41	22.428745683455688	1.0316172105937184E-5	1.0316172105937184E-5	2.1663961422468085E-4	1.0316172105937184E-5
42-43	23.379785852772955	1.0316172105937184E-5	1.0316172105937184E-5	2.4500908751600814E-4	1.0316172105937184E-5
44-45	24.183949521731947	1.0316172105937184E-5	1.0316172105937184E-5	2.656414317278825E-4	1.0316172105937184E-5
46-47	24.897444354051853	1.2895215132421481E-5	1.0316172105937184E-5	2.7595760383381966E-4	1.0316172105937184E-5
48-49	25.56356474501827	1.5474258158905777E-5	1.0316172105937184E-5	2.96589948045694E-4	1.0316172105937184E-5
50-51	26.230703857980146	1.5474258158905777E-5	1.0316172105937184E-5	3.22380378310537E-4	1.0316172105937184E-5
52-53	26.92934598151053	1.5474258158905777E-5	1.0316172105937184E-5	3.4817080857537996E-4	1.0316172105937184E-5
54-55	27.653438101626264	1.5474258158905777E-5	1.0316172105937184E-5	3.9717262607858155E-4	1.0316172105937184E-5
56-57	28.414867027636404	1.5474258158905777E-5	1.0316172105937184E-5	4.410163575288146E-4	1.0316172105937184E-5
58-59	29.200683384504984	1.8053301185390072E-5	1.0316172105937184E-5	4.642277447671733E-4	1.0316172105937184E-5
60-61	29.991799674792993	2.3211387238358663E-5	1.0316172105937184E-5	5.029133901644377E-4	1.0316172105937184E-5
62-63	30.76051181830993	2.8369473291327258E-5	1.0316172105937184E-5	5.544942506941237E-4	1.0316172105937184E-5
64-65	31.491587986941376	3.6106602370780144E-5	1.0316172105937184E-5	5.828637239854509E-4	1.0316172105937184E-5
66-67	32.16707546217998	3.6106602370780144E-5	1.0316172105937184E-5	6.344445845151369E-4	1.0316172105937184E-5
68-69	32.78097538994615	3.6106602370780144E-5	1.0316172105937184E-5	6.653931008329483E-4	1.0316172105937184E-5
70-71	33.35099290061668	3.6106602370780144E-5	1.0316172105937184E-5	6.963416171507599E-4	1.0316172105937184E-5
72-73	33.89172795676451	3.6106602370780144E-5	1.0316172105937184E-5	7.118158753096657E-4	1.0316172105937184E-5
74-75	34.415712128455326	3.6106602370780144E-5	1.0316172105937184E-5	7.479224776804458E-4	1.0316172105937184E-5
76-77	34.924075036534724	3.6106602370780144E-5	1.0316172105937184E-5	7.969242951836475E-4	1.0316172105937184E-5
78-79	35.41566900685592	3.6106602370780144E-5	1.0316172105937184E-5	8.717165429516921E-4	1.0316172105937184E-5
80-81	35.89861028782326	3.6106602370780144E-5	1.0316172105937184E-5	0.001010984866381844	1.0316172105937184E-5
82-83	36.37868367294516	3.6106602370780144E-5	1.0316172105937184E-5	0.0010831980711234043	1.0316172105937184E-5
84-85	36.86323427676103	3.6106602370780144E-5	1.0316172105937184E-5	0.0011089885013882473	1.0316172105937184E-5
86-87	37.347774564404794	3.6106602370780144E-5	1.0316172105937184E-5	0.0011141465874412158	1.0316172105937184E-5
88	37.71016879527123	3.6106602370780144E-5	1.0316172105937184E-5	0.0011141465874412158	1.0316172105937184E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	9890	0.0	50.611435	9
GCTTGCT	2260	0.0	48.247383	7
GTTGCTG	2430	0.0	42.744392	8
GCCTGCT	2120	0.0	41.678898	7
CCTGCTG	6275	0.0	40.595577	8
CTTGCTG	7120	0.0	40.464443	8
CTGCTGA	8445	0.0	38.345543	9
TGCTTGC	1365	0.0	34.089035	6
TTTGCTG	3435	0.0	34.069496	8
ATTGCTG	1490	0.0	32.174145	8
ACTTGCT	1775	0.0	31.24481	7
TCGCGAC	1245	0.0	30.956842	6
GACACGC	1280	0.0	29.74309	2
TACCCGA	14970	0.0	28.853989	6
ACTGCTG	1340	0.0	28.76088	8
GCTGAAC	19035	0.0	28.111021	10-11
TGCCAAA	2175	0.0	28.092878	6
ACCCGAC	16110	0.0	27.97804	7
TGAACCG	17065	0.0	27.789394	12-13
ATTAGCC	550	0.0	27.346212	3
>>END_MODULE