##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248484_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19473176
Sequences flagged as poor quality	0
Sequence length	100
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.683249717457493	31.0	30.0	33.0	23.0	34.0
2	30.189623459470607	31.0	30.0	34.0	25.0	34.0
3	30.466658032567466	31.0	30.0	34.0	26.0	34.0
4	33.854985802007846	35.0	35.0	37.0	28.0	37.0
5	33.65437574230316	35.0	35.0	37.0	28.0	37.0
6	33.41224888020321	35.0	33.0	37.0	28.0	37.0
7	33.508529168534196	35.0	33.0	37.0	28.0	37.0
8	33.48816474518589	35.0	33.0	37.0	28.0	37.0
9	34.74047489736651	37.0	34.0	39.0	27.0	39.0
10-11	35.20758776072275	37.0	35.0	39.0	28.5	39.0
12-13	35.47944079589277	38.0	35.0	39.0	30.0	39.0
14-15	36.26023068861494	39.0	36.0	40.0	30.0	41.0
16-17	35.95043833630426	38.0	35.0	40.0	29.0	41.0
18-19	35.5438225895971	38.0	34.0	40.0	27.0	41.0
20-21	35.141101559396375	37.5	34.0	40.0	27.0	41.0
22-23	34.83166793131228	37.0	34.0	39.0	27.0	41.0
24-25	34.55962930751512	37.0	33.0	39.0	25.0	41.0
26-27	34.281883525317085	37.0	33.0	39.0	25.0	41.0
28-29	34.1070899271901	37.0	33.0	39.0	25.0	40.0
30-31	33.98722658286455	36.5	33.0	39.0	25.0	40.0
32-33	33.8384182426123	36.0	32.0	39.0	25.0	40.0
34-35	33.634041462984776	36.0	31.5	39.0	24.0	40.0
36-37	33.349079318134855	35.0	31.0	39.0	23.5	40.0
38-39	33.06861507850594	35.0	30.5	39.0	23.0	40.0
40-41	32.76279367577225	35.0	30.0	38.0	22.0	40.0
42-43	32.46393867646448	35.0	30.0	38.0	22.0	40.0
44-45	32.07007272465467	35.0	28.5	38.0	21.5	40.0
46-47	31.917904454825447	35.0	28.5	38.0	21.5	40.0
48-49	31.940833149148347	35.0	29.0	37.5	21.5	39.0
50-51	31.770078774001732	34.0	28.5	37.0	21.5	39.0
52-53	31.63498219294069	34.0	29.0	37.0	21.0	39.0
54-55	31.37482129263352	34.0	28.5	36.0	21.0	39.0
56-57	31.639912128355437	34.0	29.5	36.0	22.5	39.0
58-59	31.76878286315494	34.0	30.0	36.0	23.0	39.0
60-61	31.572872216632767	34.0	30.0	35.0	23.0	38.0
62-63	31.3316109298247	34.0	30.0	35.0	22.5	37.0
64-65	31.128105579695884	34.0	29.0	35.0	22.0	37.0
66-67	30.94519196560438	34.0	29.0	35.0	22.0	36.0
68-69	30.801476348798985	33.0	29.0	35.0	22.0	36.0
70-71	30.69362791667882	33.0	29.0	35.0	21.0	35.0
72-73	30.619549040177112	33.0	29.0	35.0	21.0	35.0
74-75	30.545478534164126	33.0	29.0	35.0	20.0	35.0
76-77	30.419811976228225	33.0	29.0	35.0	20.0	35.0
78-79	30.28732811740622	33.0	29.0	35.0	20.0	35.0
80-81	30.18894493635758	33.0	29.0	35.0	20.0	35.0
82-83	30.073814692580193	33.0	29.0	35.0	19.0	35.0
84-85	29.958877534922912	33.0	29.0	35.0	18.0	35.0
86-87	29.7723635322764	33.0	28.5	35.0	17.0	35.0
88-89	29.568587322376175	33.0	28.0	35.0	15.0	35.0
90-91	29.28296960906634	33.0	27.5	35.0	11.0	35.0
92-93	28.980628686352958	33.0	27.0	35.0	10.0	35.0
94-95	28.70834354396016	33.0	26.5	35.0	10.0	35.0
96-97	28.374123075763297	33.0	25.5	35.0	8.0	35.0
98-99	27.983764410078766	33.0	25.0	35.0	8.0	35.0
100	26.176328863868946	31.0	20.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2064712223290215
1101	2	0.3087311505142303
1101	3	0.35486308912008724
1101	4	0.2541118147138448
1101	5	0.15974816964501315
1101	6	0.11789095491674573
1101	7	0.18972106124053312
1101	8	0.2086578642301049
1101	9	0.2725369833098341
1101	10-11	0.45316001824059526
1101	12-13	0.5260014383987368
1101	14-15	0.7171608499191251
1101	16-17	0.7775701752322917
1101	18-19	0.7269132339521391
1101	20-21	0.6666694104928084
1101	22-23	0.6797057068309798
1101	24-25	0.7004435540237139
1101	26-27	0.7369045955231002
1101	28-29	0.7517251776848184
1101	30-31	0.7141606890002805
1101	32-33	0.7597712027615415
1101	34-35	0.7890471762997322
1101	36-37	0.7559441847917583
1101	38-39	0.7366605938903987
1101	40-41	0.6933418619157763
1101	42-43	0.7330081290802966
1101	44-45	0.6676303717267089
1101	46-47	0.7330876144026988
1101	48-49	0.7320571341245596
1101	50-51	0.684518068701184
1101	52-53	0.5867856135153211
1101	54-55	0.5496389914411246
1101	56-57	0.5644246873127265
1101	58-59	0.5467779419038266
1101	60-61	0.5494737278404145
1101	62-63	0.47129408440523335
1101	64-65	0.49691804516447036
1101	66-67	0.5478502315012115
1101	68-69	0.49503401072811215
1101	70-71	0.5241732061003361
1101	72-73	0.5267785493724446
1101	74-75	0.472227494314307
1101	76-77	0.466486512783991
1101	78-79	0.4663819112062484
1101	80-81	0.4773287865545228
1101	82-83	0.4473228683899073
1101	84-85	0.44883541835638496
1101	86-87	0.44717044554923646
1101	88-89	0.4295700528328439
1101	90-91	0.42215686359616456
1101	92-93	0.4269506665905425
1101	94-95	0.40427245505814113
1101	96-97	0.3502374194717994
1101	98-99	0.33739593248730415
1101	100	0.3504709209953205
1102	1	0.30547755141728317
1102	2	0.34074139210772003
1102	3	0.35251926608134454
1102	4	0.30278789235003956
1102	5	0.21029020801220355
1102	6	0.09572976892304297
1102	7	0.19774135218048627
1102	8	0.3134551896643387
1102	9	0.25972474132918677
1102	10-11	0.37497091422568474
1102	12-13	0.527772466061208
1102	14-15	0.5866647527025037
1102	16-17	0.5953800209318558
1102	18-19	0.5894898978559482
1102	20-21	0.5387127430796639
1102	22-23	0.5175095135885002
1102	24-25	0.5307711716974168
1102	26-27	0.501441570951144
1102	28-29	0.5295333560202238
1102	30-31	0.5224368303715679
1102	32-33	0.4669289693861316
1102	34-35	0.5598253807900235
1102	36-37	0.5071161858350308
1102	38-39	0.48952824273142426
1102	40-41	0.4554252328466859
1102	42-43	0.5343046830256739
1102	44-45	0.4753918120748324
1102	46-47	0.5165009456036387
1102	48-49	0.5270712757935101
1102	50-51	0.5877837600436671
1102	52-53	0.5390076248590212
1102	54-55	0.5458763410849947
1102	56-57	0.48328926884294177
1102	58-59	0.5042119519125734
1102	60-61	0.5414218867540015
1102	62-63	0.5208566928197875
1102	64-65	0.5084131338240567
1102	66-67	0.5006841520274854
1102	68-69	0.5303189441455594
1102	70-71	0.5640441056800043
1102	72-73	0.5120124068147014
1102	74-75	0.5001145713216424
1102	76-77	0.4825069251509113
1102	78-79	0.5185406039962963
1102	80-81	0.517441397708069
1102	82-83	0.5754368936659517
1102	84-85	0.5523993256517663
1102	86-87	0.5618622492350696
1102	88-89	0.5592285037603801
1102	90-91	0.5484102004331106
1102	92-93	0.5342288628387095
1102	94-95	0.5446045299863016
1102	96-97	0.5354683914329428
1102	98-99	0.4965773040343109
1102	100	0.4635318011842564
1103	1	0.18171702691632063
1103	2	0.219841855466278
1103	3	0.2561252659740454
1103	4	0.1930836965364051
1103	5	0.12046143195448877
1103	6	0.06159189434941226
1103	7	0.1378970155824959
1103	8	0.20471407206041192
1103	9	0.09392159114236165
1103	10-11	0.21236293655719862
1103	12-13	0.33173538744975417
1103	14-15	0.3561832900005655
1103	16-17	0.3940706200388675
1103	18-19	0.36373731803079323
1103	20-21	0.32754806379956136
1103	22-23	0.30463980854723616
1103	24-25	0.3430930818103377
1103	26-27	0.3116622699608129
1103	28-29	0.3183925010638404
1103	30-31	0.2962224172580221
1103	32-33	0.31055104121669785
1103	34-35	0.3437305192705793
1103	36-37	0.2970339183037112
1103	38-39	0.23021199803376646
1103	40-41	0.24856473385425915
1103	42-43	0.265308287123851
1103	44-45	0.2255308681609307
1103	46-47	0.23456698270288712
1103	48-49	0.25636230864598275
1103	50-51	0.32156171556200164
1103	52-53	0.2868870258200502
1103	54-55	0.31911169595755595
1103	56-57	0.26019497772420763
1103	58-59	0.25795783047745147
1103	60-61	0.3384620965463583
1103	62-63	0.3436150983390043
1103	64-65	0.30397307904596005
1103	66-67	0.28343919804936846
1103	68-69	0.33101440582380803
1103	70-71	0.37512212900173836
1103	72-73	0.33487312252371026
1103	74-75	0.36677630061285527
1103	76-77	0.3616390204299087
1103	78-79	0.32311148063437045
1103	80-81	0.3465040304720688
1103	82-83	0.3892155284452059
1103	84-85	0.33050238247087904
1103	86-87	0.3720488652966125
1103	88-89	0.3287418964098876
1103	90-91	0.3024537436231185
1103	92-93	0.2804477971084509
1103	94-95	0.2612889068848716
1103	96-97	0.23869366223664912
1103	98-99	0.21492142542147263
1103	100	0.13896561635412397
1104	1	0.19734662850132167
1104	2	0.21226468863689263
1104	3	0.21737986809378995
1104	4	0.18277452473950007
1104	5	0.1356424604507822
1104	6	0.07366137269061568
1104	7	0.16645160840803186
1104	8	0.22531338412844804
1104	9	0.16464461842661393
1104	10-11	0.2028220936248033
1104	12-13	0.2812261485008989
1104	14-15	0.3447345399110091
1104	16-17	0.3067080329627103
1104	18-19	0.3067867051015867
1104	20-21	0.2711222358848815
1104	22-23	0.30619983779267557
1104	24-25	0.36878826777071083
1104	26-27	0.3781412049427928
1104	28-29	0.24971078646612455
1104	30-31	0.28990798041208876
1104	32-33	0.34531259967467065
1104	34-35	0.36279325679165453
1104	36-37	0.34601877349769694
1104	38-39	0.3354889336423952
1104	40-41	0.28744814924663586
1104	42-43	0.2791784971671447
1104	44-45	0.2585114117042622
1104	46-47	0.3206752332609213
1104	48-49	0.3480816733002996
1104	50-51	0.38339765936295933
1104	52-53	0.30713663261188273
1104	54-55	0.3570684302620215
1104	56-57	0.3348339599527499
1104	58-59	0.31869904827728845
1104	60-61	0.3565796443123759
1104	62-63	0.33722037496697155
1104	64-65	0.3149171517320539
1104	66-67	0.2960926434558431
1104	68-69	0.3438870729118122
1104	70-71	0.3769983728441133
1104	72-73	0.35951730719555997
1104	74-75	0.3423233171535607
1104	76-77	0.3276438340776586
1104	78-79	0.3689387899585803
1104	80-81	0.39361339178250887
1104	82-83	0.4305316399703365
1104	84-85	0.44215209459284566
1104	86-87	0.4186496396470645
1104	88-89	0.49432920639025824
1104	90-91	0.48835164604424364
1104	92-93	0.44033554274201947
1104	94-95	0.44741685257661246
1104	96-97	0.44402277623115793
1104	98-99	0.4039982924310479
1104	100	0.4489860207270695
1105	1	0.11666635221677168
1105	2	0.15771320734750205
1105	3	0.16577607467001343
1105	4	0.12721783306955814
1105	5	0.13991395425626507
1105	6	0.04755374759653819
1105	7	0.08409146611619178
1105	8	0.11296494810478919
1105	9	0.06469135580947949
1105	10-11	0.19606959048032024
1105	12-13	0.27768876273370324
1105	14-15	0.2916986124947769
1105	16-17	0.31750285019136726
1105	18-19	0.3064600440389995
1105	20-21	0.2563789948829722
1105	22-23	0.242918733473779
1105	24-25	0.23401724184622452
1105	26-27	0.21652728432259494
1105	28-29	0.22794883981158875
1105	30-31	0.23908289251528458
1105	32-33	0.19300495284791452
1105	34-35	0.22879655190148185
1105	36-37	0.298675651708173
1105	38-39	0.260665194336994
1105	40-41	0.21230133990901834
1105	42-43	0.25625492172071773
1105	44-45	0.22909623496617826
1105	46-47	0.18433250578125637
1105	48-49	0.2665592881258192
1105	50-51	0.27767787274640554
1105	52-53	0.3040717986282395
1105	54-55	0.3392216637648211
1105	56-57	0.3158803135616246
1105	58-59	0.28174128445493096
1105	60-61	0.34671541110893145
1105	62-63	0.3317142456589224
1105	64-65	0.32406245084099794
1105	66-67	0.2774314165484988
1105	68-69	0.31578204147418276
1105	70-71	0.3129963466849901
1105	72-73	0.30659210457882935
1105	74-75	0.3306341452242165
1105	76-77	0.3031291049799165
1105	78-79	0.3708688338077586
1105	80-81	0.2942306530916312
1105	82-83	0.3514468118494847
1105	84-85	0.3296141519623603
1105	86-87	0.32052170021693627
1105	88-89	0.33699339721463417
1105	90-91	0.33422672517370344
1105	92-93	0.33435770910086404
1105	94-95	0.3340857830375441
1105	96-97	0.29868009230193593
1105	98-99	0.3101052984609396
1105	100	0.3886404978388569
1106	1	-0.009233908042553196
1106	2	0.041313541541285304
1106	3	0.059568692715128435
1106	4	0.020833054286818253
1106	5	-0.05621821597525667
1106	6	-0.05683852131205214
1106	7	0.050146849033929186
1106	8	-0.017018090557591847
1106	9	-0.013211014019809397
1106	10-11	0.07443441472932477
1106	12-13	0.15878867782065953
1106	14-15	0.15798380785290078
1106	16-17	0.1545381638382608
1106	18-19	0.07302687381242379
1106	20-21	0.10248766692772904
1106	22-23	0.08122184779544739
1106	24-25	0.10665012379039496
1106	26-27	0.09616888374360144
1106	28-29	0.12304372806846686
1106	30-31	0.14477699221486517
1106	32-33	0.14697509218896698
1106	34-35	0.2272656174838943
1106	36-37	0.21730101192419937
1106	38-39	0.2058192504848364
1106	40-41	0.21370831461559447
1106	42-43	0.19789553091620604
1106	44-45	0.17835433575524462
1106	46-47	0.1537858262461711
1106	48-49	0.19946202202488905
1106	50-51	0.19744626453088543
1106	52-53	0.22826571883970104
1106	54-55	0.20420282782269084
1106	56-57	0.20822435118447657
1106	58-59	0.24490077808754762
1106	60-61	0.23404259166690267
1106	62-63	0.2550925368288084
1106	64-65	0.25415232384500186
1106	66-67	0.23336234002204037
1106	68-69	0.22840127870416183
1106	70-71	0.2731507148969783
1106	72-73	0.19103953328448497
1106	74-75	0.1740346249902096
1106	76-77	0.18967194561451706
1106	78-79	0.19692530650845796
1106	80-81	0.2039116224121571
1106	82-83	0.16381281002694337
1106	84-85	0.13696374003628264
1106	86-87	0.18791746193928205
1106	88-89	0.18855162868631936
1106	90-91	0.1877362402649041
1106	92-93	0.13271207005176677
1106	94-95	0.12094329795625214
1106	96-97	0.08922459922787596
1106	98-99	0.04828124358510877
1106	100	0.05974351709968673
1107	1	0.11337156309416585
1107	2	0.11275139734598127
1107	3	0.1167962970970926
1107	4	0.11540961720680798
1107	5	0.08316723024278616
1107	6	0.03268774154452103
1107	7	0.09322390315497842
1107	8	0.08747352418545518
1107	9	-0.026902745817380946
1107	10-11	0.10317062150308942
1107	12-13	0.18231404350780167
1107	14-15	0.2416558643915394
1107	16-17	0.2651227320139711
1107	18-19	0.1857500567319974
1107	20-21	0.17695685753069057
1107	22-23	0.16785804406399762
1107	24-25	0.23577552784727374
1107	26-27	0.20297588606113948
1107	28-29	0.2501244104825133
1107	30-31	0.22488762265819418
1107	32-33	0.25192928908002443
1107	34-35	0.27908241118177557
1107	36-37	0.2699409265699586
1107	38-39	0.2666663354018084
1107	40-41	0.29271178714805046
1107	42-43	0.34185627330516866
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2216	36-37	0.19036073682623567
2216	38-39	0.17776273700255274
2216	40-41	0.15329693404008538
2216	42-43	0.18191784452944404
2216	44-45	0.22500468988975086
2216	46-47	0.16429530327798858
2216	48-49	0.09788646057273809
2216	50-51	0.0962785549334022
2216	52-53	0.16190537279653583
2216	54-55	0.17765536464680665
2216	56-57	0.17913182179966824
2216	58-59	0.10379153663252083
2216	60-61	0.11912844073270179
2216	62-63	0.08036081349336044
2216	64-65	0.1095247437655722
2216	66-67	0.10065525388851526
2216	68-69	0.1103129831889973
2216	70-71	0.0632280661622957
2216	72-73	0.005747492240271157
2216	74-75	0.008259490088395438
2216	76-77	-0.083052704836863
2216	78-79	-0.07956475857986689
2216	80-81	-0.16803851652705504
2216	82-83	-0.27033073197754476
2216	84-85	-0.26993684822693
2216	86-87	-0.263765554022676
2216	88-89	-0.33807079351358027
2216	90-91	-0.3946240051082128
2216	92-93	-0.37499356472826406
2216	94-95	-0.32920617542838926
2216	96-97	-0.35265122313100505
2216	98-99	-0.28004672740374303
2216	100	0.09509802977119719
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	698.0
6	4307.0
7	13514.0
8	29348.0
9	50352.0
10	69014.0
11	80282.0
12	82737.0
13	84603.0
14	88846.0
15	94568.0
16	101720.0
17	111070.0
18	123597.0
19	137251.0
20	151706.0
21	170599.0
22	191020.0
23	218730.0
24	281784.0
25	354739.0
26	336946.0
27	570188.0
28	492051.0
29	744539.0
30	783181.0
31	967491.0
32	1258167.0
33	1934468.0
34	2549437.0
35	4491435.0
36	2210637.0
37	604382.0
38	87334.0
39	2428.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.75574211400969	27.919162933725676	26.361191321898954	28.963903630365678
2	14.937800141374403	28.567145009927792	29.201460577827433	27.293594270870376
3	13.46399786044146	30.608371228196162	26.38931112213026	29.53831978923212
4	12.539137174042608	29.274811594658374	26.649784981123265	31.536266250175753
5	12.417688046129511	29.37640776999168	27.558773931768904	30.6471302521099
6	12.228699128526891	25.938846567231383	28.732298499350183	33.10015580489154
7	17.192281354206223	30.025622518853567	24.019071432111108	28.7630246948291
8	14.716792987440774	28.080879051265185	21.739740861993955	35.46258709930009
9	11.599458660467096	26.473288178569334	23.277995330602465	38.64925783036111
10-11	17.879861440821244	39.41015163797425	21.728450497425335	20.981536423779165
12-13	17.249888275780435	41.08476418005335	20.37157273679982	21.293774807366393
14-15	19.060663788284465	40.029702012133	19.470624374152404	21.439009825430134
16-17	19.037500589273407	39.642483844665165	20.065267871108986	21.254747694952442
18-19	19.988264347109112	37.35259631228432	21.29171367800538	21.367425662601185
20-21	18.43403433238365	36.832904880358406	22.86997077002397	21.863090017233976
22-23	18.53811367311293	37.77378738558773	23.840246801206533	19.847852140092805
24-25	18.505081040709538	37.82440008758715	23.03148443787495	20.63903443382836
26-27	20.675813231493414	38.17828175537468	21.34317997228598	19.80272504084593
28-29	19.840536027610494	39.31681200847771	22.485045582703098	18.357606381208694
30-31	20.185429752799273	38.74295070762205	22.419912451213253	18.651707088365423
32-33	20.90696070542187	38.97685892949119	21.667422407343025	18.44875795774391
34-35	20.98082098248481	39.12681128831916	21.451078602769886	18.441289126426142
36-37	21.533767776116886	38.855935098511445	21.120110885024804	18.490186240346866
38-39	22.058190716818462	39.043456176841694	20.933202852177093	17.965150254162747
40-41	22.424161705716074	39.32477356078255	20.845551960204244	17.405512773297133
42-43	22.666210239186384	39.6310388762928	20.988790463582525	16.713960420938292
44-45	22.98735629787439	39.27596456936941	20.899280309538895	16.837398823217306
46-47	23.091621286682233	38.79584200075309	21.23271795776397	16.879818754800702
48-49	22.94688871202281	38.94206839386774	21.59727306365944	16.51376983045001
50-51	23.22338431080785	38.73234391760235	21.90407204248552	16.140199729104282
52-53	23.31724545795831	38.715141849007665	22.178472531426408	15.789140161607621
54-55	23.294939571851618	38.86309388412022	22.19969197998951	15.642274564038658
56-57	23.179509119054252	39.028749547066845	21.770458199918327	16.02128313396058
58-59	22.945741581345672	38.917712013654544	21.90050885377426	16.236037551225525
60-61	22.916967882398396	38.63480210553295	22.254818354043497	16.193411658025163
62-63	23.03268559815208	38.070217052387	22.472105541517127	16.424991807943794
64-65	22.8467441403724	37.95439406306465	22.516522691032257	16.682339105530698
66-67	22.444580649155213	37.77172653644267	22.92466759147902	16.859025222923094
68-69	22.229429866097444	37.97030100954116	22.593700604270385	17.206568520091007
70-71	21.818055816493196	38.15750475779505	22.70999394624677	17.314445479464986
72-73	21.740931920352114	38.67831448566966	22.644811480312363	16.93594211366586
74-75	21.49635228882261	39.267363239492184	22.480894992510567	16.755389479174646
76-77	20.906756756097497	40.06898852157267	22.479516545251705	16.544738177078123
78-79	20.40324022881417	40.87479039896637	22.219651997771788	16.50231737444768
80-81	19.8932946047599	41.98072907350864	21.78325884799954	16.34271747373191
82-83	19.458564281237965	42.83596088583035	21.64119866606731	16.06427616686437
84-85	18.967662248824922	43.8785987533383	21.497299947252728	15.656439050584051
86-87	18.58236786446328	44.66822441952984	21.1595619804773	15.589845735529572
88-89	18.301844605236177	45.273103757741445	20.952747932811608	15.472303704210768
90-91	18.191058502311694	45.75139255943512	20.676394961484277	15.38115397676891
92-93	18.20188485198588	46.00929562054535	20.292046321886755	15.496773205582015
94-95	18.290946692549976	46.12591779464509	19.931427751540358	15.651707761264575
96-97	18.42694809354733	45.87484518989859	19.808282739053062	15.889923977501008
98-99	18.657817131867045	45.43471835064489	19.67919974175676	16.2282647757313
100	19.199473609261897	44.40030181296788	19.410037093949686	16.990187483820534
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1028785.0
1	1027790.0
2	654778.0
3	171885.0
4	58129.0
5	53756.5
6	46933.5
7	35595.0
8	25648.5
9	20895.0
10	21239.5
11	25134.0
12	32330.0
13	43113.5
14	58057.0
15	77724.5
16	100081.5
17	122913.0
18	146588.0
19	170019.5
20	191188.5
21	208391.0
22	222352.5
23	233247.5
24	240927.0
25	245751.0
26	247121.5
27	245011.0
28	238071.5
29	227542.0
30	215043.5
31	200853.5
32	186588.0
33	173072.0
34	160699.0
35	149819.0
36	141291.0
37	134871.5
38	131188.5
39	131435.0
40	137388.0
41	151166.5
42	175458.0
43	215560.0
44	272727.0
45	344092.5
46	422722.0
47	500255.5
48	571859.5
49	642169.0
50	724259.0
51	826454.0
52	940302.0
53	1036005.5
54	1074761.5
55	1026163.0
56	887762.0
57	690539.5
58	484385.0
59	310377.5
60	185346.5
61	108136.5
62	65715.0
63	43678.5
64	32141.5
65	25710.5
66	21581.5
67	18402.5
68	15932.5
69	13991.0
70	12223.5
71	10762.0
72	9703.0
73	8790.0
74	7945.5
75	7249.5
76	6894.5
77	6768.5
78	6443.0
79	6044.5
80	5748.0
81	5450.5
82	5301.0
83	5254.0
84	5124.5
85	5031.0
86	4961.0
87	4894.0
88	4832.0
89	4778.0
90	4699.5
91	4566.5
92	4441.5
93	4349.0
94	4234.5
95	4095.0
96	3918.0
97	3616.5
98	3165.5
99	2630.0
100	1963.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013367105602085658
2	3.1325141825863434E-4
3	0.0
4	1.0270538303561782E-5
5	1.0270538303561782E-5
6	0.0038257755180767634
7	3.646041097764433E-4
8	0.0
9	0.0
10-11	2.6960163046849675E-4
12-13	1.7973442031233118E-5
14-15	4.3393024332548526E-4
16-17	2.156813043747974E-4
18-19	1.9000495861589296E-4
20-21	8.216430642849425E-5
22-23	6.932613354904203E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	1.7203151658465982E-4
32-33	3.0811614910685345E-5
34-35	1.027053830356178E-4
36-37	1.6689624743287894E-4
38-39	6.239352019413782E-4
40-41	0.0017742354919402976
42-43	0.001042459637811521
44-45	4.2109207044603304E-4
46-47	5.571767029682266E-4
48-49	9.62862965958917E-4
50-51	0.0
52-53	1.1554355591507004E-4
54-55	4.647418582361706E-4
56-57	5.340679917852126E-4
58-59	4.955534731468559E-4
60-61	3.337924948657579E-5
62-63	2.2081657352657832E-4
64-65	1.6176097828109805E-4
66-67	1.8743732404000253E-4
68-69	1.1554355591507004E-4
70-71	6.80423162610968E-4
72-73	6.008264907583642E-4
74-75	3.825775518076763E-4
76-77	4.185244358701426E-4
78-79	8.6529285207508E-4
80-81	0.0011040828676328915
82-83	4.0825389756658087E-4
84-85	6.701526243074063E-4
86-87	0.0013043583645523462
88-89	0.001175976635757824
90-91	0.0011246239442400151
92-93	6.932613354904203E-4
94-95	7.086671429457629E-4
96-97	6.419086439726114E-4
98-99	3.1838668741041525E-4
100	9.705658696865884E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9473176E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.91848050049465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.46633728832072	80.95308444684082
2	3.1743894224907176	5.327798737045275
3	0.20780487611647752	0.5231600833286498
4	0.05007136873871736	0.16807652724532524
5	0.025294497080324694	0.10613378800025232
6	0.017587060011518316	0.08855276115825773
7	0.01145332282913892	0.06728018139520753
8	0.006834491589276499	0.045883211933239555
9	0.0072873539428004955	0.05503893027741956
>10	0.027653720983370187	0.41286834412349577
>50	0.002283198405795262	0.13081740642164852
>100	0.001625847654146869	0.2806332230762753
>500	4.3005761733737075E-4	0.2627921953834601
>1k	5.135748163153798E-4	0.948029090702926
>5k	1.4667698141807974E-4	0.8500840557831253
>10k+	2.8724242194373825E-4	9.779767017284703
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	464226	2.383925457254636	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124237	0.6379904336098025	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	108725	0.5583321385273774	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	99644	0.511698759360055	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	75048	0.3853916793028523	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56467	0.28997324319361156	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56178	0.2884891504087469	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55103	0.2829687360705824	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52414	0.2691599973214436	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45678	0.23456882431504752	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44190	0.22692754381719754	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43640	0.22410314578371807	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41473	0.21297501753180886	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39590	0.20330530571900546	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34162	0.1754310647631388	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32262	0.1656740533747551	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30925	0.15880819851882405	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30609	0.1571854534668613	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29752	0.15278452780378507	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28508	0.14639625297896963	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26653	0.13687032870241608	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25950	0.13326023448871413	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22836	0.11726900635006841	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21718	0.11152777543837739	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20269	0.10408677043744687	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0029681855697293548	0.0	0.0	0.0	0.0
2	0.006937748624055984	0.0	0.0	2.0541076607123564E-5	0.0
3	0.011631384628783718	0.0	0.0	4.108215321424713E-5	0.0
4	0.01855886271453614	0.0	0.0	5.64879606695898E-5	0.0
5	0.030637015759524794	0.0	0.0	5.64879606695898E-5	0.0
6	0.05241055696307577	0.0	0.0	6.162322982137069E-5	0.0
7	0.07850285952327447	0.0	0.0	6.162322982137069E-5	0.0
8	0.17900521209277828	0.0	0.0	6.162322982137069E-5	0.0
9	0.5539928360941225	0.0	0.0	6.675849897315157E-5	0.0
10-11	1.3878372998836963	0.0	0.0	6.675849897315157E-5	0.0
12-13	2.4520910199753754	0.0	0.0	7.702903727671336E-5	0.0
14-15	3.618485243495976	0.0	0.0	1.0013774845972736E-4	0.0
16-17	4.839636841982017	0.0	0.0	1.2067882506685093E-4	0.0
18-19	6.134577122909997	0.0	0.0	1.412199016739745E-4	0.0
20-21	7.752274205296557	0.0	0.0	1.643286128569885E-4	0.0
22-23	9.56327565672903	0.0	0.0	1.694638820087694E-4	0.0
24-25	11.42180659179581	0.0	0.0	1.7459915116055028E-4	0.0
26-27	13.295599546781686	0.0	0.0	2.0027549691945472E-4	0.0
28-29	15.269181051924967	5.135269151780891E-6	0.0	2.156813043747974E-4	0.0
30-31	17.345057118571724	5.135269151780891E-6	0.0	2.2081657352657832E-4	0.0
32-33	19.643654943600367	5.135269151780891E-6	0.0	2.3879001555781142E-4	0.0
34-35	21.81964308236109	5.135269151780891E-6	0.0	2.4649291928548276E-4	0.0
36-37	23.616509705453286	5.135269151780891E-6	0.0	2.618987267408254E-4	0.0
38-39	25.07621252948158	5.135269151780891E-6	0.0	3.183866874104152E-4	0.0
40-41	26.468799953330674	5.135269151780891E-6	0.0	3.4919830232110055E-4	0.0
42-43	27.681219026624113	5.135269151780891E-6	2.5676345758904454E-6	3.954157246871286E-4	0.0
44-45	28.715423719274142	5.135269151780891E-6	5.135269151780891E-6	4.3393024332548526E-4	0.0
46-47	29.637212234922544	5.135269151780891E-6	5.135269151780891E-6	4.5703895450849926E-4	0.0
48-49	30.501778446412644	5.135269151780891E-6	5.135269151780891E-6	4.981211077227463E-4	0.0
50-51	31.356567105437755	5.135269151780891E-6	5.135269151780891E-6	5.469061646646649E-4	0.0
52-53	32.23526814526814	5.135269151780891E-6	5.135269151780891E-6	6.136646636378165E-4	0.0
54-55	33.13672869797921	5.135269151780891E-6	5.135269151780891E-6	7.343434887046674E-4	0.0
56-57	34.07363544601045	1.0270538303561782E-5	5.135269151780891E-6	7.77993276494805E-4	0.0
58-59	35.02737047105208	1.0270538303561782E-5	5.135269151780891E-6	8.139401605572712E-4	0.0
60-61	35.97994749289998	1.0270538303561782E-5	5.135269151780891E-6	8.652928520750801E-4	0.0
62-63	36.89711940158092	1.0270538303561782E-5	5.135269151780891E-6	9.063750052893272E-4	0.0
64-65	37.77660613759153	1.0270538303561782E-5	5.135269151780891E-6	9.782687734142597E-4	0.0
66-67	38.598963004288564	1.2838172879452227E-5	5.135269151780891E-6	0.0010886770601775488	0.0
68-69	39.34903839003971	1.5405807455342673E-5	1.0270538303561782E-5	0.0011657060974542622	2.5676345758904454E-6
70-71	40.06074047705418	1.5405807455342673E-5	1.2838172879452227E-5	0.0012119235198202903	5.135269151780891E-6
72-73	40.74383911489323	1.5405807455342673E-5	1.7973442031233118E-5	0.0012632762113380992	5.135269151780891E-6
74-75	41.39896850929709	1.5405807455342673E-5	2.0541076607123564E-5	0.0013480081523424838	5.135269151780891E-6
76-77	42.033194790618644	1.5405807455342673E-5	2.0541076607123564E-5	0.001463551708257554	5.135269151780891E-6
78-79	42.64854895780739	1.5405807455342673E-5	2.0541076607123564E-5	0.0016176097828109804	5.135269151780891E-6
80-81	43.24645091278382	1.5405807455342673E-5	2.0541076607123564E-5	0.0017434238770296124	5.135269151780891E-6
82-83	43.83961044669858	1.5405807455342673E-5	2.0541076607123564E-5	0.001810182376002764	5.135269151780891E-6
84-85	44.426178862657025	1.5405807455342673E-5	2.0541076607123564E-5	0.001833291087185778	5.135269151780891E-6
86-87	45.01214645212471	1.5405807455342673E-5	2.0541076607123564E-5	0.0018538321637929017	5.135269151780891E-6
88	45.4474709210249	1.5405807455342673E-5	2.0541076607123564E-5	0.0018589674329446825	5.135269151780891E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	14685	0.0	50.85658	9
CCTGCTG	8590	0.0	48.31283	8
GCCTGCT	2920	0.0	47.80423	7
GCTTGCT	3335	0.0	46.788055	7
CTGCTGA	11700	0.0	44.469166	9
GTTGCTG	3645	0.0	43.840584	8
CTTGCTG	10050	0.0	43.11812	8
ATTGCTG	2110	0.0	40.76276	8
TTTGCTG	4295	0.0	37.205803	8
TGCTTGC	1525	0.0	35.75159	6
ACTTGCT	2455	0.0	34.459877	7
ACTGCTG	1645	0.0	33.142635	8
GCTGCTG	2215	0.0	32.464794	8
CGCTTGC	2465	0.0	31.461126	6
GCTGAAC	27680	0.0	30.308844	10-11
TGAACCG	25665	0.0	29.73072	12-13
CCGCTTG	1505	0.0	29.668415	5
GTGCTGA	4155	0.0	29.636534	9
CCCTGCT	5230	0.0	29.565619	7
AGTTGCC	735	0.0	28.775908	3
>>END_MODULE