##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248483_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22524548
Sequences flagged as poor quality	0
Sequence length	100
%GC	26
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.069037656160738	31.0	29.0	33.0	16.0	34.0
2	29.693748971122528	31.0	30.0	34.0	23.0	34.0
3	30.27678637546911	31.0	30.0	34.0	25.0	34.0
4	33.878151428388264	35.0	35.0	37.0	28.0	37.0
5	33.664517796317156	35.0	35.0	37.0	28.0	37.0
6	33.41268970191988	35.0	33.0	37.0	27.0	37.0
7	33.6191224791725	35.0	35.0	37.0	28.0	37.0
8	33.59995081810299	35.0	35.0	37.0	28.0	37.0
9	34.864406113720904	37.0	34.0	39.0	27.0	39.0
10-11	35.694699800413304	38.5	35.0	39.0	30.5	39.0
12-13	35.979064907318005	39.0	35.0	39.0	31.0	39.0
14-15	37.021172966489715	39.5	36.5	41.0	31.0	41.0
16-17	36.72637228946836	39.0	36.0	40.5	30.5	41.0
18-19	35.920179841122675	37.5	35.5	40.0	30.0	41.0
20-21	35.25271534860544	37.0	34.0	39.5	29.0	41.0
22-23	34.70408271899618	35.5	34.0	39.0	28.0	40.0
24-25	34.288346518651565	35.0	33.0	39.0	26.5	40.0
26-27	34.01680730729869	35.0	33.0	38.5	25.0	40.0
28-29	33.83932216975009	35.0	33.0	38.0	25.0	40.0
30-31	33.71230758992367	35.0	33.0	38.0	25.0	40.0
32-33	33.59117290611115	35.0	33.0	38.0	25.0	40.0
34-35	33.44852500480809	35.0	33.0	38.0	25.0	40.0
36-37	33.19292564716504	35.0	32.0	38.0	25.0	40.0
38-39	32.97165883195525	35.0	31.0	38.0	24.5	40.0
40-41	32.74624780483941	35.0	31.0	37.5	24.0	40.0
42-43	32.5439176404339	35.0	30.5	37.0	23.5	39.5
44-45	32.252732085900234	35.0	30.0	37.0	23.0	39.0
46-47	32.16966779977116	35.0	30.0	37.0	23.0	39.0
48-49	32.21590009264558	35.0	30.0	37.0	23.0	39.0
50-51	32.099950418538924	35.0	30.0	36.0	23.0	39.0
52-53	32.00358650926092	35.0	30.0	36.0	23.0	39.0
54-55	31.850378595832424	35.0	29.5	35.0	23.0	39.0
56-57	32.10265666596284	35.0	30.0	35.0	24.5	39.0
58-59	32.21181685865572	35.0	30.0	35.0	25.0	39.0
60-61	32.07438302424537	35.0	30.0	35.0	25.0	38.0
62-63	31.88844943747595	34.0	30.0	35.0	25.0	37.5
64-65	31.733833393682303	34.0	30.0	35.0	25.0	37.0
66-67	31.585965587411565	34.0	30.0	35.0	24.0	36.5
68-69	31.47455631518111	34.0	30.0	35.0	24.0	36.0
70-71	31.385422428898462	34.0	30.0	35.0	24.0	35.5
72-73	31.317108139084525	34.0	30.0	35.0	24.0	35.0
74-75	31.24841424120919	34.0	30.0	35.0	24.0	35.0
76-77	31.145539213483886	34.0	30.0	35.0	24.0	35.0
78-79	31.048034748577418	34.0	30.0	35.0	23.0	35.0
80-81	30.98716189554614	34.0	30.0	35.0	23.0	35.0
82-83	30.924270600235793	34.0	30.0	35.0	23.0	35.0
84-85	30.86344363047818	34.0	30.0	35.0	23.0	35.0
86-87	30.762825029829678	34.0	30.0	35.0	21.5	35.0
88-89	30.658646735108736	34.0	30.0	35.0	20.0	35.0
90-91	30.497488073900527	34.0	29.0	35.0	20.0	35.0
92-93	30.317001189102662	34.0	29.0	35.0	19.5	35.0
94-95	30.140932040012522	34.0	29.0	35.0	18.0	35.0
96-97	29.91133160585509	34.0	29.0	35.0	16.0	35.0
98-99	29.62788751632219	34.0	28.0	35.0	15.0	35.0
100	28.22002390458623	33.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.44669375742877904
1101	2	0.35760856068119296
1101	3	0.417251447006727
1101	4	0.29434337014735235
1101	5	0.250249967752012
1101	6	0.2217033836532778
1101	7	0.3122855815246055
1101	8	0.30128845254623826
1101	9	0.3543797576390162
1101	10-11	0.4587894765066025
1101	12-13	0.5338102823402409
1101	14-15	0.6877711648040972
1101	16-17	0.7425217515302478
1101	18-19	0.7040213480787259
1101	20-21	0.6187956838541666
1101	22-23	0.6155919233545077
1101	24-25	0.6466540829714305
1101	26-27	0.6558774919277113
1101	28-29	0.6966461697663462
1101	30-31	0.6796103357763172
1101	32-33	0.6921286357452558
1101	34-35	0.7450852350428647
1101	36-37	0.7096472256040443
1101	38-39	0.6745363554879731
1101	40-41	0.6851603490913618
1101	42-43	0.7043823190726428
1101	44-45	0.6433536850297159
1101	46-47	0.6795092201295176
1101	48-49	0.6824780551825071
1101	50-51	0.638014788403396
1101	52-53	0.6219099309073002
1101	54-55	0.6071553209378635
1101	56-57	0.5520499521871685
1101	58-59	0.583199882248401
1101	60-61	0.6070141847756574
1101	62-63	0.5512568980284662
1101	64-65	0.5504750398402294
1101	66-67	0.5409822982824544
1101	68-69	0.5264155281662148
1101	70-71	0.5134433527595661
1101	72-73	0.5239926750552151
1101	74-75	0.5150591011291361
1101	76-77	0.5375687130271274
1101	78-79	0.5100324777003884
1101	80-81	0.4851277084855923
1101	82-83	0.5156278652946469
1101	84-85	0.4716863365442947
1101	86-87	0.46410804186322707
1101	88-89	0.4778919740739731
1101	90-91	0.4487096440113021
1101	92-93	0.397945070153515
1101	94-95	0.38724310009961016
1101	96-97	0.36402902055792197
1101	98-99	0.39442279151398907
1101	100	0.4065293251663178
1102	1	0.3295070805824629
1102	2	0.3358446481509141
1102	3	0.2821480217529597
1102	4	0.21905895485203786
1102	5	0.19327986619056503
1102	6	0.09897805582009767
1102	7	0.13827243240129405
1102	8	0.24665200819905664
1102	9	0.2059081388833306
1102	10-11	0.2966818232801387
1102	12-13	0.38479590344114456
1102	14-15	0.48502105627827774
1102	16-17	0.4912614212138138
1102	18-19	0.4673237170434774
1102	20-21	0.4105612327767716
1102	22-23	0.367989012949991
1102	24-25	0.373357243156768
1102	26-27	0.37678068265448417
1102	28-29	0.39005637603310106
1102	30-31	0.3639968906834099
1102	32-33	0.34343556941210807
1102	34-35	0.3994493095908922
1102	36-37	0.3521191878930878
1102	38-39	0.3308104413728472
1102	40-41	0.30276469891978763
1102	42-43	0.36906963407888327
1102	44-45	0.3200463713581527
1102	46-47	0.3592308233409085
1102	48-49	0.3491755439652522
1102	50-51	0.4453744660788459
1102	52-53	0.44950904770444566
1102	54-55	0.4498407055070004
1102	56-57	0.41402217097040506
1102	58-59	0.45756049099603047
1102	60-61	0.4235112014524063
1102	62-63	0.4238722144457405
1102	64-65	0.43780205260124205
1102	66-67	0.4014294457569001
1102	68-69	0.4334001398827887
1102	70-71	0.45437221879757317
1102	72-73	0.44139270933971275
1102	74-75	0.40080950214085576
1102	76-77	0.421189258629564
1102	78-79	0.4639032699286112
1102	80-81	0.45966579266528385
1102	82-83	0.45189818999311626
1102	84-85	0.4370852447201301
1102	86-87	0.45758357780342607
1102	88-89	0.4467134578447798
1102	90-91	0.4523706080224592
1102	92-93	0.4266212091714898
1102	94-95	0.3990810837929857
1102	96-97	0.4012259402285032
1102	98-99	0.4014068903798602
1102	100	0.3624011339159239
1103	1	0.23626415751979835
1103	2	0.2532293104445067
1103	3	0.24247643470098268
1103	4	0.22107595105384803
1103	5	0.17656996061305108
1103	6	0.14933534231433043
1103	7	0.1931629862837454
1103	8	0.25541715582694025
1103	9	0.18751069710538815
1103	10-11	0.21686628163828203
1103	12-13	0.33034141141492057
1103	14-15	0.37960218395942746
1103	16-17	0.4123062289331543
1103	18-19	0.35415072753456656
1103	20-21	0.31851454287028247
1103	22-23	0.2863973251864209
1103	24-25	0.3071593956319063
1103	26-27	0.2792082414388517
1103	28-29	0.3182029562673776
1103	30-31	0.28698810786097795
1103	32-33	0.2816683741718933
1103	34-35	0.3261279951878606
1103	36-37	0.29379560843106844
1103	38-39	0.24187053519015933
1103	40-41	0.2397166848463499
1103	42-43	0.2875537336447209
1103	44-45	0.23568476941812833
1103	46-47	0.2653121287527398
1103	48-49	0.2928380149542633
1103	50-51	0.2955671081813094
1103	52-53	0.28565673686795634
1103	54-55	0.29051915315491783
1103	56-57	0.2864719253051291
1103	58-59	0.29559897506235444
1103	60-61	0.3288070090706938
1103	62-63	0.3263642314371644
1103	64-65	0.32707439291425544
1103	66-67	0.3139722095616655
1103	68-69	0.361214243494711
1103	70-71	0.3565038784652259
1103	72-73	0.37776598207542733
1103	74-75	0.3765600703224301
1103	76-77	0.346850636842543
1103	78-79	0.34793754709125224
1103	80-81	0.3653021175833544
1103	82-83	0.3506303256020118
1103	84-85	0.3148062356626511
1103	86-87	0.36703973690719494
1103	88-89	0.3521713600211953
1103	90-91	0.32021101550693487
1103	92-93	0.31543309689643806
1103	94-95	0.30631021582689755
1103	96-97	0.29502887186980686
1103	98-99	0.2878890765675379
1103	100	0.29816763779222555
1104	1	0.10699752957600595
1104	2	0.12538227087280518
1104	3	0.13587534084591724
1104	4	0.11571869311413252
1104	5	0.046178445277902824
1104	6	0.04521969444132168
1104	7	0.07435474588613289
1104	8	0.1173918755340182
1104	9	0.023984034415761357
1104	10-11	0.07511537176924321
1104	12-13	0.13318707550864417
1104	14-15	0.1948502421786955
1104	16-17	0.18072473701752045
1104	18-19	0.18466402090902534
1104	20-21	0.1810696129841034
1104	22-23	0.14304762201126664
1104	24-25	0.15186952715460933
1104	26-27	0.15297759178158543
1104	28-29	0.061447578024441896
1104	30-31	0.06828524520187784
1104	32-33	0.12679972515203275
1104	34-35	0.16439825734293834
1104	36-37	0.11925446471479262
1104	38-39	0.061359021116373924
1104	40-41	0.05114496973997973
1104	42-43	0.08344578458933682
1104	44-45	0.061441358798369095
1104	46-47	0.09251146428938029
1104	48-49	0.1369450620930195
1104	50-51	0.14484222439541838
1104	52-53	0.151056062519892
1104	54-55	0.15923077587216028
1104	56-57	0.17428272819891077
1104	58-59	0.156616690116131
1104	60-61	0.1729050253282267
1104	62-63	0.1860222561879361
1104	64-65	0.1774738176612516
1104	66-67	0.17233066604162417
1104	68-69	0.20362576249706166
1104	70-71	0.19502567874372545
1104	72-73	0.19941885082356592
1104	74-75	0.23423196964913018
1104	76-77	0.17517300259961388
1104	78-79	0.1934773033940438
1104	80-81	0.17412304821245783
1104	82-83	0.19549434733753657
1104	84-85	0.1859126647735181
1104	86-87	0.2066354195657354
1104	88-89	0.2522894209622031
1104	90-91	0.2185616940586108
1104	92-93	0.23114551882428103
1104	94-95	0.18666163143079473
1104	96-97	0.20145038797841863
1104	98-99	0.16667333968263875
1104	100	0.24160464109375823
1105	1	0.051900649502997
1105	2	0.09499200217059922
1105	3	0.12912541852635329
1105	4	0.08167928957109893
1105	5	0.044515657504064166
1105	6	0.009739000379177298
1105	7	0.09587693480187198
1105	8	0.06270502567105751
1105	9	0.04751411980306841
1105	10-11	0.17409604562227088
1105	12-13	0.21345148498075162
1105	14-15	0.2405681126716317
1105	16-17	0.22437748587618955
1105	18-19	0.2086785092820591
1105	20-21	0.19761926275236874
1105	22-23	0.13875491210595214
1105	24-25	0.1560304144925979
1105	26-27	0.1373852975237071
1105	28-29	0.10436414853118947
1105	30-31	0.09123688762666404
1105	32-33	0.10204723144489947
1105	34-35	0.15106314341331029
1105	36-37	0.16700716188334752
1105	38-39	0.1613053168253913
1105	40-41	0.14658585344856334
1105	42-43	0.1273071229613052
1105	44-45	0.12524549649220518
1105	46-47	0.10796657296359768
1105	48-49	0.15967355156048058
1105	50-51	0.19916920743818878
1105	52-53	0.1813549343793639
1105	54-55	0.18825465751684334
1105	56-57	0.21869791827836593
1105	58-59	0.1903416449421229
1105	60-61	0.2135292535873745
1105	62-63	0.2266975210820128
1105	64-65	0.22705184130889933
1105	66-67	0.1773940788335544
1105	68-69	0.20106929122659167
1105	70-71	0.20627503512560708
1105	72-73	0.22416702135923217
1105	74-75	0.270005570511362
1105	76-77	0.2276274889542833
1105	78-79	0.23400621690705847
1105	80-81	0.2350944322504489
1105	82-83	0.25863983642793187
1105	84-85	0.24198498766769916
1105	86-87	0.25314971857655877
1105	88-89	0.3041243366642732
1105	90-91	0.2772144495812725
1105	92-93	0.29247057647492625
1105	94-95	0.2779913363176121
1105	96-97	0.24629023535681682
1105	98-99	0.23133183302254423
1105	100	0.30318061022506626
1106	1	-0.04454773761932174
1106	2	0.0415331444209599
1106	3	0.06524271714427954
1106	4	0.054166084470395504
1106	5	-0.024571998142256746
1106	6	-0.012331016702788133
1106	7	0.05527981929338921
1106	8	0.05439530144192872
1106	9	0.044080931938850654
1106	10-11	0.09390660000369877
1106	12-13	0.11835114117228329
1106	14-15	0.12350809264084006
1106	16-17	0.1139846657852388
1106	18-19	0.042628750862903075
1106	20-21	0.053522689916725597
1106	22-23	0.05279449645353651
1106	24-25	0.08206781083321601
1106	26-27	0.04002733643767442
1106	28-29	0.07924140752146513
1106	30-31	0.043669966015528416
1106	32-33	0.08229305580920965
1106	34-35	0.17383794169303002
1106	36-37	0.13217168467176776
1106	38-39	0.1440277757460109
1106	40-41	0.17944636392218172
1106	42-43	0.16081910905563745
1106	44-45	0.1704994829740727
1106	46-47	0.15836054753769702
1106	48-49	0.14633692065110182
1106	50-51	0.20258151438611804
1106	52-53	0.24813492539839643
1106	54-55	0.18894897515809106
1106	56-57	0.18831184394078804
1106	58-59	0.21999165475185833
1106	60-61	0.21654185702474393
1106	62-63	0.22258523060130742
1106	64-65	0.21564717750461426
1106	66-67	0.2090063920457581
1106	68-69	0.21481558375658594
1106	70-71	0.23317459292356446
1106	72-73	0.19959338676178007
1106	74-75	0.1678799288314785
1106	76-77	0.16380306744873607
1106	78-79	0.2052988701392735
1106	80-81	0.20451199740658055
1106	82-83	0.21032867618742657
1106	84-85	0.16616189001877046
1106	86-87	0.2119791482682416
1106	88-89	0.20906814468914803
1106	90-91	0.19889096003471707
1106	92-93	0.15388003957466623
1106	94-95	0.17742717357988624
1106	96-97	0.17853423813564362
1106	98-99	0.19337890756459686
1106	100	0.22062526086448386
1107	1	-0.04193472298748091
1107	2	-0.047107597824073366
1107	3	0.02831079406455217
1107	4	-3.218904454911353E-4
1107	5	-0.051553996680205216
1107	6	-0.0802882460713974
1107	7	-7.025013351125153E-4
1107	8	-0.02977016561523982
1107	9	-0.08126297902810364
1107	10-11	0.03487935804982101
1107	12-13	0.08480109097406796
1107	14-15	0.08519898730070707
1107	16-17	0.09814868081107875
1107	18-19	0.055024027691793265
1107	20-21	0.09981701285953903
1107	22-23	0.06801679219024237
1107	24-25	0.10828639689924557
1107	26-27	0.05729354850549839
1107	28-29	0.10355447844949595
1107	30-31	0.10831610836984851
1107	32-33	0.10753392141035789
1107	34-35	0.17489636636575057
1107	36-37	0.13914706043617997
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2216	12-13	-0.014176413932922571
2216	14-15	-0.013491974544457719
2216	16-17	-0.01269787414530299
2216	18-19	0.07393614689942041
2216	20-21	0.0769004647598095
2216	22-23	0.14815481763462657
2216	24-25	0.22670131595683785
2216	26-27	0.26281337161341156
2216	28-29	0.29477310330085515
2216	30-31	0.2769580936092524
2216	32-33	0.32221579671876555
2216	34-35	0.29137689438046266
2216	36-37	0.3728304946407519
2216	38-39	0.35845765379184513
2216	40-41	0.3896144056487998
2216	42-43	0.44321832515328197
2216	44-45	0.4582579699375273
2216	46-47	0.36814601480793385
2216	48-49	0.3145435823553413
2216	50-51	0.317007084654648
2216	52-53	0.3605981240074101
2216	54-55	0.38460017263888346
2216	56-57	0.3090695640586105
2216	58-59	0.21848217706443762
2216	60-61	0.22363887300054586
2216	62-63	0.20035362380296462
2216	64-65	0.20431464789695042
2216	66-67	0.19215051662988358
2216	68-69	0.18863675213183484
2216	70-71	0.1805282402547448
2216	72-73	0.13789970022181564
2216	74-75	0.10679515179772281
2216	76-77	0.034967027697753394
2216	78-79	0.034281807924152474
2216	80-81	-0.005994669079100845
2216	82-83	-0.1037696773909289
2216	84-85	-0.11855395822087189
2216	86-87	-0.14350298262139916
2216	88-89	-0.19551300915748726
2216	90-91	-0.23297679346436695
2216	92-93	-0.17713410475760227
2216	94-95	-0.17752338984463023
2216	96-97	-0.1962362601976011
2216	98-99	-0.13196309825764274
2216	100	0.16939311048026084
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	548.0
6	4080.0
7	12459.0
8	24171.0
9	39488.0
10	55438.0
11	65517.0
12	68256.0
13	70463.0
14	75191.0
15	82237.0
16	91769.0
17	105083.0
18	122289.0
19	137750.0
20	156729.0
21	179037.0
22	200621.0
23	233631.0
24	336600.0
25	435373.0
26	348786.0
27	772693.0
28	465153.0
29	868211.0
30	675687.0
31	826254.0
32	1122490.0
33	2141379.0
34	2555359.0
35	7286878.0
36	2113095.0
37	730710.0
38	117358.0
39	3754.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.77438806738109	36.44607595986625	24.523003528245344	24.256532444507318
2	11.837541508784522	41.64430836909707	24.949538166283226	21.56861195583519
3	10.219077426104176	45.523834706916205	22.005640246365875	22.25144762061374
4	8.92338074205802	45.625305833023226	21.550219036601227	23.901094388317524
5	8.889483598072644	44.7373461167789	22.576288767259616	23.79688151788884
6	8.765788432671318	41.663516638762616	23.97334175116689	25.597353177399185
7	11.879892321479032	46.41239488369419	19.71812858513114	21.98958420969564
8	10.424036033930625	44.0299978494574	17.573595705449893	27.97237041116208
9	8.47209453437201	42.604117960546866	18.305455008464545	30.618332496616578
10-11	11.520469824997452	56.11782376075231	17.24006671499336	15.121639699256878
12-13	11.106644871773868	57.31281207347914	16.482889353182436	15.097653701564557
14-15	12.289442431039886	56.1748107384546	16.017630884374697	15.51811594613081
16-17	12.327338511015542	55.85984998641257	16.531877099298082	15.280934403273804
18-19	12.742710138497179	54.29960671272555	17.545182574957707	15.412500573819568
20-21	11.73325807315474	53.83764534377813	18.569934073646294	15.859162509420841
22-23	11.830309645043473	54.53915959101128	19.253202719008648	14.377328044936602
24-25	11.792736085092583	54.555230142686995	18.746251867074093	14.90578190514633
26-27	13.260261648757613	54.851602349578776	17.628597919034824	14.259538082628783
28-29	12.762471415630628	55.71127553813733	18.28802069635315	13.238232349878897
30-31	12.985178851671	55.455936142076034	18.207595967822243	13.351289038430725
32-33	13.425879361484839	55.71794264650782	17.604730609475947	13.251447382531396
34-35	13.469322202941994	55.83850529079094	17.415944361066156	13.276228145200907
36-37	13.850696419145189	55.68023233357795	17.12633704351393	13.342734203762923
38-39	14.225313466028332	55.77551963013639	16.994347088462096	13.004819815373192
40-41	14.427272957530853	55.91957975199795	16.99574081190311	12.657406478568085
42-43	14.55031883029902	56.02757050706134	17.201694712949063	12.22041594969057
44-45	14.778594387453728	55.57930014563299	17.14326920340578	12.498836263507501
46-47	14.860597270137857	55.16599056645133	17.419944307056383	12.553467856354425
48-49	14.748045412431374	55.27442630113934	17.730084779041245	12.247443507388038
50-51	14.959636481939615	55.18810410757188	17.88498708164976	11.96727232883874
52-53	15.059823360057765	55.25209737539141	18.009484375039193	11.678594889511633
54-55	14.990003074440303	55.45035888818472	17.97816148469939	11.581476552675596
56-57	14.891136119729293	55.65949265803286	17.60982628048953	11.839544941748317
58-59	14.683826619032148	55.67291040082873	17.746542347380597	11.896720632758537
60-61	14.617071357207982	55.564307739790955	17.99865044944766	11.819970453553404
62-63	14.652312040941387	55.18918672220502	18.17724820709616	11.98125302975743
64-65	14.54770449899555	55.10122963631765	18.177031677787557	12.174034186899242
66-67	14.33707772285906	54.85929665163037	18.46172431045582	12.341901315054747
68-69	14.257964842667672	54.89465945976472	18.274758719597965	12.572616977969652
70-71	14.094343699981321	54.9253248616306	18.343651589348838	12.636679849039249
72-73	14.147790048012766	55.11575744482901	18.32502191176814	12.411430595390089
74-75	14.122776251783062	55.30455544569972	18.203211884674754	12.36945641784246
76-77	13.81595446976107	55.690074509669685	18.20662374177333	12.287347278795908
78-79	13.510495820981806	56.1413850008099	18.053171255248674	12.294947922959619
80-81	13.197425900199391	56.79071113481587	17.817791153257296	12.194071811727436
82-83	12.833658713260682	57.357448829577905	17.84263829357252	11.966254163588898
84-85	12.485507644174536	58.11678144491871	17.716085745244555	11.681625165662203
86-87	12.198188946100851	58.718865144042866	17.48942572206565	11.593520187790634
88-89	11.976614997204127	59.268895122659934	17.354686157350752	11.399803722785183
90-91	11.866833541549566	59.76061456046471	17.14520882938643	11.2273430685993
92-93	11.87343218405578	60.07199758217406	16.823935233697462	11.230635000072699
94-95	11.927239760987977	60.22792182163423	16.537568830942813	11.307269586434971
96-97	12.051451434648397	60.070774846631224	16.44035232657724	11.437421392143134
98-99	12.247117722628948	59.801052748738115	16.307918195430453	11.643911333202484
100	12.637302067945807	59.04015004573054	16.19908123361522	12.123466652708428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3501700.0
1	3271647.0
2	1842988.5
3	388683.5
4	126982.5
5	116878.5
6	99980.5
7	73533.5
8	50902.5
9	38992.0
10	37411.0
11	42381.5
12	52540.0
13	68378.5
14	90280.5
15	118080.5
16	149369.0
17	182127.5
18	214694.0
19	245811.5
20	272625.0
21	293600.5
22	309306.0
23	321083.5
24	329510.0
25	332829.0
26	330429.5
27	324207.5
28	312798.0
29	296137.0
30	276667.0
31	255278.5
32	233793.5
33	212717.0
34	193034.5
35	175381.5
36	159649.0
37	145565.0
38	134086.0
39	126222.0
40	122556.5
41	124705.5
42	135455.5
43	158208.5
44	194804.5
45	243672.0
46	301665.5
47	362161.0
48	420175.5
49	473865.0
50	527663.0
51	586976.0
52	647832.0
53	694754.0
54	707997.5
55	671933.0
56	584153.5
57	463422.0
58	336116.5
59	224810.5
60	141446.5
61	86518.0
62	53786.0
63	35647.0
64	25800.0
65	20206.0
66	16688.5
67	14251.0
68	12384.5
69	10746.0
70	9378.0
71	8340.5
72	7382.5
73	6597.5
74	6031.5
75	5595.5
76	5290.5
77	4939.0
78	4684.0
79	4372.0
80	4047.0
81	3909.5
82	3846.5
83	3804.5
84	3735.0
85	3566.5
86	3389.0
87	3334.0
88	3253.5
89	3168.0
90	3136.0
91	3073.5
92	2997.5
93	2910.0
94	2753.5
95	2574.5
96	2455.0
97	2322.5
98	2093.5
99	1787.5
100	1378.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01400694033904698
2	4.3952047339640285E-4
3	0.0
4	8.879201482755614E-6
5	0.0
6	0.004164345495412383
7	4.128828689481361E-4
8	0.0
9	0.0
10-11	3.3740965634471333E-4
12-13	2.2198003706889037E-5
14-15	4.2842147154295834E-4
16-17	2.441780407757794E-4
18-19	2.042216341033791E-4
20-21	8.879201482755615E-5
22-23	6.65940111206671E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	1.997820333620013E-4
32-33	3.7736606301711364E-5
34-35	1.4650682446546763E-4
36-37	1.6870482817235666E-4
38-39	6.104451019394485E-4
40-41	0.00186241251100799
42-43	0.0011609555938702967
44-45	5.283124882239591E-4
46-47	6.681599115773599E-4
48-49	0.0010255477712582733
50-51	0.0
52-53	1.220890203878897E-4
54-55	5.460708911894703E-4
56-57	5.993461000860039E-4
58-59	5.616094937842926E-4
60-61	5.3275208896533684E-5
62-63	2.441780407757794E-4
64-65	1.886830315085568E-4
66-67	2.1532063595682365E-4
68-69	1.287484214999564E-4
70-71	6.681599115773599E-4
72-73	5.416312904480924E-4
74-75	3.4628885782746893E-4
76-77	3.9512446598262484E-4
78-79	7.924687323359386E-4
80-81	0.0010943615827496294
82-83	4.883560815515588E-4
84-85	7.702707286290494E-4
86-87	0.0013984742335340092
88-89	0.0012564070098099193
90-91	0.0012763852131461196
92-93	8.013479338186941E-4
94-95	7.014569171376935E-4
96-97	7.169955197325158E-4
98-99	3.773660630171136E-4
100	9.545141593962285E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.2524548E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.77098871482605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.54317014178362	54.11854800749124
2	7.868428534355915	9.406075062608055
3	1.1343896578786246	2.03410774317836
4	0.217126555663102	0.5191147563291327
5	0.06039448817977482	0.18049191357155084
6	0.037773233802829244	0.13546461188108358
7	0.022041681514828996	0.0922217167955044
8	0.01542116645081805	0.073739069272104
9	0.01388197595914743	0.07467654855543188
>10	0.07480007161170471	0.8131894341421624
>50	0.006330906104160024	0.26234662723384417
>100	0.004124744154707233	0.49680523724022774
>500	5.797574935903622E-4	0.2492812636410224
>1k	8.540076682556138E-4	1.1202956256077143
>5k	1.4849508235157904E-4	0.648325352480483
>10k+	5.345822964656845E-4	29.775317029971998
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1658482	7.362997916761749	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	474248	2.1054717723969425	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	389877	1.7308982182461554	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	364432	1.617932577381797	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	248432	1.1029388913819713	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	211090	0.9371553204974413	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	204638	0.9085110165140717	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	193918	0.8609184965665015	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	159421	0.7077655897911914	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152960	0.6790813294011494	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	148902	0.6610654295926383	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	144303	0.6406477057830416	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	141278	0.6272179135403738	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	133557	0.5929397562161958	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	121771	0.5406146218783169	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	113824	0.5053331147865875	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107408	0.4768486364299075	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	106531	0.4729551065797192	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	94283	0.4185788766993238	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78641	0.3491346419026921	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73982	0.3284505420486129	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72260	0.32080554957196034	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69361	0.3079351470227061	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66790	0.29652093351662373	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57434	0.254984028980293	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56337	0.25011378696700154	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55976	0.24851109109936412	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54235	0.24078174620862539	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43612	0.19361986753296892	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43142	0.19153325518452136	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42544	0.18887837394117743	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41864	0.1858594454370405	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40224	0.1785785002211809	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34636	0.15377001127836173	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33688	0.14956126977553555	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30961	0.13745447855379828	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30574	0.13573635306688508	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28892	0.1282689446198876	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28767	0.12771399452721538	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28294	0.12561406337654366	No Hit
TAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26794	0.11895466226447696	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24267	0.10773579119101523	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24165	0.1072829519153947	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24070	0.10686118984496382	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24021	0.1066436494086363	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23019	0.10219516946577574	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0019401055239821018	0.0	0.0	4.439600741377807E-6	0.0
2	0.0047015371851190976	0.0	0.0	2.6637604448266842E-5	0.0
3	0.007809257704083562	0.0	0.0	3.9956406672400265E-5	0.0
4	0.01240868407215097	0.0	0.0	5.3275208896533684E-5	0.0
5	0.0202046229740104	0.0	0.0	7.103361186204491E-5	0.0
6	0.03433587213381596	0.0	0.0	7.547321260342271E-5	0.0
7	0.05371028976918871	0.0	0.0	7.991281334480053E-5	0.0
8	0.1197271527934767	0.0	0.0	7.991281334480053E-5	0.0
9	0.3633058474691701	0.0	0.0	8.435241408617833E-5	0.0
10-11	0.8701018107000416	0.0	0.0	8.435241408617833E-5	0.0
12-13	1.495510586938304	4.439600741377807E-6	0.0	8.657221445686723E-5	0.0
14-15	2.1345023216448116	4.439600741377807E-6	4.439600741377807E-6	1.0655041779306737E-4	0.0
16-17	2.776128959391327	4.439600741377807E-6	4.439600741377807E-6	1.1986922001720079E-4	2.2198003706889035E-6
18-19	3.4492590039986597	4.439600741377807E-6	4.439600741377807E-6	1.4872662483615654E-4	4.439600741377807E-6
20-21	4.303520319253465	4.439600741377807E-6	4.439600741377807E-6	1.7314442891373447E-4	4.439600741377807E-6
22-23	5.289753206146467	4.439600741377807E-6	4.439600741377807E-6	1.7758402965511227E-4	4.439600741377807E-6
24-25	6.333518879046984	4.439600741377807E-6	4.439600741377807E-6	1.8424343076717898E-4	4.439600741377807E-6
26-27	7.432224611122052	4.439600741377807E-6	4.439600741377807E-6	2.1310083558613474E-4	4.439600741377807E-6
28-29	8.661940741274808	1.3318802224133421E-5	4.439600741377807E-6	2.2198003706889034E-4	4.439600741377807E-6
30-31	10.052987522768493	1.3318802224133421E-5	8.879201482755614E-6	2.3529883929302377E-4	4.439600741377807E-6
32-33	11.629496405432864	1.3318802224133421E-5	1.3318802224133421E-5	2.6637604448266844E-4	4.439600741377807E-6
34-35	13.12130436535286	1.3318802224133421E-5	1.3318802224133421E-5	2.7525524596542405E-4	4.439600741377807E-6
36-37	14.327399599761115	1.3318802224133421E-5	1.3318802224133421E-5	2.885740481895575E-4	4.439600741377807E-6
38-39	15.279429802542541	1.3318802224133421E-5	1.3318802224133421E-5	3.4628885782746893E-4	4.439600741377807E-6
40-41	16.164202273892464	1.5538602594822324E-5	1.3318802224133421E-5	3.818056637584914E-4	4.439600741377807E-6
42-43	16.88928008677466	1.7758402965511228E-5	1.3318802224133421E-5	4.395204733964029E-4	4.439600741377807E-6
44-45	17.476528274840412	1.7758402965511228E-5	1.3318802224133421E-5	4.5949867673260303E-4	4.439600741377807E-6
46-47	17.979392971614793	1.7758402965511228E-5	1.3318802224133421E-5	4.661580778446697E-4	4.439600741377807E-6
48-49	18.44567979788096	1.7758402965511228E-5	1.7758402965511228E-5	5.216530871118923E-4	4.439600741377807E-6
50-51	18.91946732959969	1.7758402965511228E-5	1.7758402965511228E-5	5.904668986032483E-4	1.3318802224133421E-5
52-53	19.424895895802216	1.7758402965511228E-5	1.7758402965511228E-5	6.592807100946044E-4	1.3318802224133421E-5
54-55	19.955212863760906	1.7758402965511228E-5	1.7758402965511228E-5	7.192153201032047E-4	1.3318802224133421E-5
56-57	20.52258939890825	1.7758402965511228E-5	1.7758402965511228E-5	7.480727249221605E-4	1.3318802224133421E-5
58-59	21.124741326662804	1.997820333620013E-5	1.7758402965511228E-5	7.791499301118051E-4	1.3318802224133421E-5
60-61	21.745575094337077	2.2198003706889034E-5	1.7758402965511228E-5	8.5018354197385E-4	1.3318802224133421E-5
62-63	22.3403151086539	2.2198003706889034E-5	1.7758402965511228E-5	9.21217153835895E-4	1.3318802224133421E-5
64-65	22.90287467699685	2.2198003706889034E-5	1.7758402965511228E-5	9.878111649565622E-4	1.5538602594822324E-5
66-67	23.41843219229083	2.2198003706889034E-5	1.7758402965511228E-5	0.0010877021816375627	1.7758402965511228E-5
68-69	23.877462491145216	2.2198003706889034E-5	1.7758402965511228E-5	0.00115429619275823	1.7758402965511228E-5
70-71	24.30515364836622	2.2198003706889034E-5	1.7758402965511228E-5	0.001169834795353052	1.7758402965511228E-5
72-73	24.712136287929063	3.107720518964465E-5	1.7758402965511228E-5	0.0011942525994306302	1.7758402965511228E-5
74-75	25.098650148273784	3.107720518964465E-5	1.7758402965511228E-5	0.0012786050135168083	1.7758402965511228E-5
76-77	25.473223702424573	3.107720518964465E-5	1.7758402965511228E-5	0.0013851554313098757	1.7758402965511228E-5
78-79	25.83880928487444	3.107720518964465E-5	1.7758402965511228E-5	0.0015383216568874102	1.7758402965511228E-5
80-81	26.19589525170494	3.107720518964465E-5	1.7758402965511228E-5	0.0017669610950683673	1.7758402965511228E-5
82-83	26.551564985898942	3.107720518964465E-5	1.7758402965511228E-5	0.001866852111749368	1.7758402965511228E-5
84-85	26.906770781815467	3.107720518964465E-5	1.7758402965511228E-5	0.0019112481191631459	1.7758402965511228E-5
86-87	27.26557265433251	3.107720518964465E-5	1.7758402965511228E-5	0.001933446122870035	1.997820333620013E-5
88	27.532659034933797	3.107720518964465E-5	1.7758402965511228E-5	0.0019489847254648572	2.2198003706889034E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	8130	0.0	47.751133	9
GCTTGCT	1965	0.0	46.40174	7
GTTGCTG	2255	0.0	39.18353	8
CTTGCTG	5770	0.0	38.44664	8
TGAAAAA	25955	0.0	37.36184	1
GCCTGCT	1950	0.0	36.39464	7
TGCTTGC	1300	0.0	35.792877	6
CCTGCTG	6535	0.0	35.38439	8
CTGCTGA	8235	0.0	34.186714	9
GTACGAC	1320	0.0	29.553497	6
TACCCGA	14160	0.0	28.910769	6
ACCCGAC	15085	0.0	28.321333	7
TTTGCTG	2795	0.0	28.081924	8
CGTTGCT	1260	0.0	27.976011	7
ACTGCTG	1210	0.0	27.96658	8
CCCGACG	17325	0.0	27.724892	8
ATGCTGA	2145	0.0	27.389046	9
ATTGCTG	1640	0.0	27.225256	8
GTGCTGA	2665	0.0	27.159243	9
GCTGAAC	16840	0.0	27.156004	10-11
>>END_MODULE