##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248483_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22524548
Sequences flagged as poor quality	0
Sequence length	100
%GC	27
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35275065231054	34.0	31.0	34.0	30.0	34.0
2	32.55846661162746	34.0	31.0	34.0	31.0	34.0
3	32.71232878901721	34.0	31.0	34.0	31.0	34.0
4	36.222235047735474	37.0	37.0	37.0	35.0	37.0
5	36.15107317580801	37.0	37.0	37.0	35.0	37.0
6	36.140814989938974	37.0	37.0	37.0	35.0	37.0
7	36.13342367624869	37.0	37.0	37.0	35.0	37.0
8	36.11687826099774	37.0	36.0	37.0	35.0	37.0
9	37.90402946154569	39.0	38.0	39.0	35.0	39.0
10-11	37.9624898355341	39.0	38.0	39.0	35.0	39.0
12-13	37.938147793243175	39.0	38.0	39.0	35.0	39.0
14-15	39.42785293627202	41.0	39.0	41.0	36.0	41.0
16-17	39.329598201038266	41.0	39.0	41.0	36.0	41.0
18-19	38.91076122814984	40.0	38.5	41.0	35.5	41.0
20-21	38.335993934262305	40.0	36.5	41.0	35.0	41.0
22-23	37.76563392082274	39.0	35.0	41.0	34.0	41.0
24-25	37.25081539927017	38.0	35.0	41.0	33.5	41.0
26-27	37.031056183680136	38.0	35.0	41.0	33.0	41.0
28-29	36.88836905850452	38.0	35.0	41.0	33.0	41.0
30-31	36.79193979830361	38.0	35.0	41.0	33.0	41.0
32-33	36.792606359958924	38.0	35.0	41.0	33.0	41.0
34-35	36.73977464497845	38.0	35.0	41.0	33.0	41.0
36-37	36.717241784385635	38.0	35.0	41.0	33.0	41.0
38-39	36.61607951023035	38.0	35.0	41.0	33.0	41.0
40-41	36.56406406024219	37.0	35.0	41.0	33.0	41.0
42-43	36.47861300035854	37.0	35.0	40.5	33.0	41.0
44-45	36.32308899161928	36.5	35.0	40.0	33.0	41.0
46-47	36.1225274087631	36.0	35.0	40.0	33.0	41.0
48-49	35.87495860072309	35.0	35.0	40.0	32.0	41.0
50-51	35.59641407676638	35.0	35.0	39.0	32.0	41.0
52-53	35.319416465093994	35.0	35.0	39.0	31.0	41.0
54-55	35.036039724304345	35.0	35.0	38.5	31.0	40.5
56-57	34.739844324512084	35.0	35.0	37.0	31.0	40.0
58-59	34.50929938749492	35.0	35.0	37.0	30.5	40.0
60-61	34.284990646649156	35.0	34.5	36.0	30.0	40.0
62-63	34.073308507677936	35.0	34.0	35.5	30.0	39.0
64-65	33.89064151254001	35.0	34.0	35.0	29.5	39.0
66-67	33.72045225946376	35.0	34.0	35.0	29.0	38.5
68-69	33.56657953802225	35.0	34.0	35.0	29.0	37.5
70-71	33.42369358532744	35.0	33.5	35.0	29.0	37.0
72-73	33.280530801328396	35.0	33.0	35.0	29.0	36.5
74-75	33.148654880888174	35.0	33.0	35.0	29.0	36.0
76-77	33.024567840384634	35.0	33.0	35.0	28.5	36.0
78-79	32.90488910143724	35.0	33.0	35.0	27.5	35.5
80-81	32.78846454543727	35.0	33.0	35.0	27.0	35.0
82-83	32.66764127741875	35.0	33.0	35.0	27.0	35.0
84-85	32.54120249161049	35.0	33.0	35.0	27.0	35.0
86-87	32.4475890925758	35.0	33.0	35.0	27.0	35.0
88-89	32.358329343612134	35.0	33.0	35.0	27.0	35.0
90-91	32.27374092035055	35.0	33.0	35.0	26.5	35.0
92-93	32.13974724376267	35.0	33.0	35.0	25.0	35.0
94-95	31.98976683128114	35.0	33.0	35.0	25.0	35.0
96-97	31.835768846504713	35.0	33.0	35.0	24.0	35.0
98-99	31.51865455857316	35.0	32.5	35.0	23.0	35.0
100	29.979646250837085	34.0	29.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2410438828756014
1101	2	0.25367755518742996
1101	3	0.24945667671220662
1101	4	0.1655802947027567
1101	5	0.14241766962752678
1101	6	0.16948636019574082
1101	7	0.2050091568072716
1101	8	0.20114089289861425
1101	9	0.24679808359904598
1101	10-11	0.2521668139421607
1101	12-13	0.2803207773409895
1101	14-15	0.36839005403718517
1101	16-17	0.39363579830344264
1101	18-19	0.39137762560586253
1101	20-21	0.40617451613808697
1101	22-23	0.411974355297545
1101	24-25	0.4054846494727613
1101	26-27	0.44615933151109033
1101	28-29	0.43558380029722343
1101	30-31	0.46527069177530933
1101	32-33	0.4407850636598809
1101	34-35	0.442365077749173
1101	36-37	0.4474000759660868
1101	38-39	0.4923041638929391
1101	40-41	0.4753061892454298
1101	42-43	0.47057179549162953
1101	44-45	0.4443377316251613
1101	46-47	0.4715042899530246
1101	48-49	0.4162966499060019
1101	50-51	0.4215333026459689
1101	52-53	0.41744950752675436
1101	54-55	0.4175114423572168
1101	56-57	0.4258215245756318
1101	58-59	0.41778759614684446
1101	60-61	0.3977000291889041
1101	62-63	0.38908689175371336
1101	64-65	0.33731569727149235
1101	66-67	0.37628163275048365
1101	68-69	0.3637706004616206
1101	70-71	0.3739004937232622
1101	72-73	0.38879796155563895
1101	74-75	0.387988782455146
1101	76-77	0.36009796618454715
1101	78-79	0.39532161502012286
1101	80-81	0.37949318628204054
1101	82-83	0.38957905968749174
1101	84-85	0.39471201262861655
1101	86-87	0.41679623870700766
1101	88-89	0.3813999725434485
1101	90-91	0.4001039556067667
1101	92-93	0.39005655677399176
1101	94-95	0.36752687724452926
1101	96-97	0.424905280057537
1101	98-99	0.5429717936366245
1101	100	0.9592678571619686
1102	1	0.24251183389717568
1102	2	0.2522393870347912
1102	3	0.23559245143781737
1102	4	0.1490418922291994
1102	5	0.14052219942335853
1102	6	0.14121265464422805
1102	7	0.20128692107187618
1102	8	0.19370394153560966
1102	9	0.20415721156161482
1102	10-11	0.23362083192387217
1102	12-13	0.27613315874135935
1102	14-15	0.3208578598483953
1102	16-17	0.3454064957851841
1102	18-19	0.3829106570814247
1102	20-21	0.38557430337044707
1102	22-23	0.32705847062882043
1102	24-25	0.33258306854425257
1102	26-27	0.3625307026532596
1102	28-29	0.3619497161247054
1102	30-31	0.3778646482662751
1102	32-33	0.3650383604804617
1102	34-35	0.37906112459872077
1102	36-37	0.3742608588542282
1102	38-39	0.410966244473002
1102	40-41	0.3848886612249771
1102	42-43	0.38937913750709896
1102	44-45	0.42138099931645456
1102	46-47	0.4274684967670055
1102	48-49	0.4338435245931791
1102	50-51	0.39794622475418606
1102	52-53	0.3690340515297095
1102	54-55	0.38209656006457493
1102	56-57	0.39524112283412904
1102	58-59	0.3670025595149795
1102	60-61	0.36624220252186035
1102	62-63	0.3491447441328006
1102	64-65	0.3356393189068996
1102	66-67	0.3463123494096365
1102	68-69	0.3579442356800797
1102	70-71	0.37391111713848346
1102	72-73	0.3816303756127013
1102	74-75	0.375320724534113
1102	76-77	0.3826470249339664
1102	78-79	0.3792342280411347
1102	80-81	0.36223091430996845
1102	82-83	0.38401078182978665
1102	84-85	0.3685629784640412
1102	86-87	0.37188831453815396
1102	88-89	0.3956598478650619
1102	90-91	0.411722786393689
1102	92-93	0.37150409778112703
1102	94-95	0.3794783426176451
1102	96-97	0.39711237786592335
1102	98-99	0.5870052623954081
1102	100	0.8908204200884278
1103	1	0.20202989278222816
1103	2	0.1884695882022953
1103	3	0.21192738331156136
1103	4	0.1296942414034845
1103	5	0.12195751717869996
1103	6	0.13274127372103806
1103	7	0.18055367421542456
1103	8	0.16989374129062185
1103	9	0.19698429303313247
1103	10-11	0.21021171104242597
1103	12-13	0.24676729874163073
1103	14-15	0.3204918488989037
1103	16-17	0.335369480767973
1103	18-19	0.32882000297530567
1103	20-21	0.34228812483753757
1103	22-23	0.3030912358129143
1103	24-25	0.27488350314044396
1103	26-27	0.3050224216381565
1103	28-29	0.31568447050741355
1103	30-31	0.3557692306672564
1103	32-33	0.35103013814339334
1103	34-35	0.3350213250981895
1103	36-37	0.3169177686907574
1103	38-39	0.35624182970160945
1103	40-41	0.3340834182333765
1103	42-43	0.33326563361098493
1103	44-45	0.3531297108717055
1103	46-47	0.3382337553303074
1103	48-49	0.32264800433436136
1103	50-51	0.3317876995319722
1103	52-53	0.34222207596501164
1103	54-55	0.35442948259068174
1103	56-57	0.31930280616230533
1103	58-59	0.2999236352715755
1103	60-61	0.3352414276892475
1103	62-63	0.30970809577110003
1103	64-65	0.29073991677992694
1103	66-67	0.28261684287480904
1103	68-69	0.33369660664808976
1103	70-71	0.3226716806897727
1103	72-73	0.31432809331663947
1103	74-75	0.343241994332395
1103	76-77	0.34907143834655585
1103	78-79	0.33820463040279236
1103	80-81	0.33143821602492807
1103	82-83	0.3203399675326821
1103	84-85	0.3250256080432621
1103	86-87	0.3505535540853373
1103	88-89	0.36980878402563633
1103	90-91	0.37857991229151366
1103	92-93	0.3605027685526352
1103	94-95	0.33858559740298233
1103	96-97	0.34673300489460246
1103	98-99	0.488292657181141
1103	100	0.876640251918321
1104	1	0.165924601955183
1104	2	0.16407385304404443
1104	3	0.16463921532749737
1104	4	0.10060131096887659
1104	5	0.11878350526666992
1104	6	0.11246069164963046
1104	7	0.13252061185952613
1104	8	0.14428168666777452
1104	9	0.1560437629674709
1104	10-11	0.17040106791358056
1104	12-13	0.17498045871401757
1104	14-15	0.2235161445811258
1104	16-17	0.2354234262424839
1104	18-19	0.22125707720077514
1104	20-21	0.2400124854180774
1104	22-23	0.23549101032824638
1104	24-25	0.2369586120664522
1104	26-27	0.2668587927931583
1104	28-29	0.2883657998520235
1104	30-31	0.30593809953204953
1104	32-33	0.2930124253223596
1104	34-35	0.28741662145415603
1104	36-37	0.2778216677656218
1104	38-39	0.2787637979317594
1104	40-41	0.25158546380894364
1104	42-43	0.2566090605821145
1104	44-45	0.2515488084269464
1104	46-47	0.28268767513657167
1104	48-49	0.2728815778608862
1104	50-51	0.26388543396812736
1104	52-53	0.25543136916283515
1104	54-55	0.2361106497997909
1104	56-57	0.2856230391291348
1104	58-59	0.2541226890695256
1104	60-61	0.27179516637346524
1104	62-63	0.2700999083914155
1104	64-65	0.24948761974549427
1104	66-67	0.24767901999238262
1104	68-69	0.23330059303135187
1104	70-71	0.25661885070309864
1104	72-73	0.2763457953323183
1104	74-75	0.30401710428931494
1104	76-77	0.3108079633070133
1104	78-79	0.31118551900456737
1104	80-81	0.2837418770180662
1104	82-83	0.28230876979016273
1104	84-85	0.24546624241371262
1104	86-87	0.25399750492889694
1104	88-89	0.2637425760684664
1104	90-91	0.25375494117517405
1104	92-93	0.254878577610711
1104	94-95	0.2086738695981154
1104	96-97	0.21880205840973588
1104	98-99	0.41574857380104646
1104	100	0.8014829085390929
1105	1	0.1684469792297918
1105	2	0.17194046580874556
1105	3	0.1765913458635211
1105	4	0.10095602255450586
1105	5	0.07770268215070075
1105	6	0.09720632638644133
1105	7	0.14705906404650904
1105	8	0.11977823346928318
1105	9	0.14954421582693556
1105	10-11	0.15980802025017482
1105	12-13	0.1911160068723916
1105	14-15	0.22896758236893078
1105	16-17	0.24973879192486947
1105	18-19	0.24879241013765352
1105	20-21	0.2461808443045186
1105	22-23	0.19456398785111162
1105	24-25	0.2051079556399884
1105	26-27	0.2192008619016974
1105	28-29	0.2194773410901476
1105	30-31	0.23247639550456967
1105	32-33	0.2007079178068949
1105	34-35	0.2036695356217635
1105	36-37	0.20431934427108445
1105	38-39	0.23641836339475475
1105	40-41	0.18834608636629468
1105	42-43	0.21756389807676868
1105	44-45	0.2267988548989308
1105	46-47	0.21603654792675542
1105	48-49	0.20553649692729437
1105	50-51	0.1900648252811621
1105	52-53	0.20423325128243874
1105	54-55	0.21235560693587274
1105	56-57	0.20820128383414271
1105	58-59	0.19396087808721063
1105	60-61	0.19718601832475713
1105	62-63	0.2220609010264738
1105	64-65	0.21519598689858555
1105	66-67	0.24325988891019534
1105	68-69	0.22798645220574087
1105	70-71	0.216764183466708
1105	72-73	0.23398175028624735
1105	74-75	0.2422268803152221
1105	76-77	0.26594528333111356
1105	78-79	0.2437740405592166
1105	80-81	0.1899557496621611
1105	82-83	0.23866684430339546
1105	84-85	0.2421329517973163
1105	86-87	0.25874324745017674
1105	88-89	0.265427046152368
1105	90-91	0.2715275879783263
1105	92-93	0.26408548677961363
1105	94-95	0.25547177577021074
1105	96-97	0.30798537371359913
1105	98-99	0.4127426536824288
1105	100	0.8383909474059514
1106	1	0.1211297440829
1106	2	0.12660081159052794
1106	3	0.1225931495898962
1106	4	0.07043254423320633
1106	5	0.06389250771281496
1106	6	0.06337819305617387
1106	7	0.08571226353306116
1106	8	0.10999875795670278
1106	9	0.11488570737013504
1106	10-11	0.10658267060257032
1106	12-13	0.11650320343693465
1106	14-15	0.1270424235730161
1106	16-17	0.1388305034255879
1106	18-19	0.1221688135946124
1106	20-21	0.15372416200178662
1106	22-23	0.12578160554868134
1106	24-25	0.1476569365385032
1106	26-27	0.15107912662711698
1106	28-29	0.13091732065446848
1106	30-31	0.18993676916959856
1106	32-33	0.15866901361629715
1106	34-35	0.1422680735217483
1106	36-37	0.10501723373383243
1106	38-39	0.11847848068322264
1106	40-41	0.086223417581067
1106	42-43	0.11233462912139913
1106	44-45	0.11470628229683655
1106	46-47	0.11918546401834362
1106	48-49	0.10369356421227849
1106	50-51	0.10122274535211062
1106	52-53	0.12078880397379521
1106	54-55	0.09817472666698279
1106	56-57	0.14132606643443069
1106	58-59	0.12355535373590953
1106	60-61	0.11804834016623289
1106	62-63	0.13679268340121808
1106	64-65	0.1254171013996057
1106	66-67	0.11735743671397358
1106	68-69	0.09244257931088384
1106	70-71	0.11224818367656297
1106	72-73	0.06397346172555274
1106	74-75	0.10871923100568637
1106	76-77	0.07379807392562299
1106	78-79	0.11954061733957388
1106	80-81	0.09491818447526867
1106	82-83	0.06873784969076269
1106	84-85	0.13847666612547016
1106	86-87	0.09281278526022163
1106	88-89	0.09351691482253699
1106	90-91	0.09294832052270863
1106	92-93	0.14037179293611501
1106	94-95	0.1417491859607054
1106	96-97	0.13923139396829498
1106	98-99	0.3152184795244324
1106	100	0.6944001514660236
1107	1	0.07724012684984416
1107	2	0.09411657034951304
1107	3	0.10722368844403007
1107	4	0.07470141599300462
1107	5	0.026214528927120284
1107	6	0.0508330939546795
1107	7	0.1070333047899581
1107	8	0.09285421359614787
1107	9	0.10433900270440688
1107	10-11	0.11084812620644158
1107	12-13	0.1223415697372019
1107	14-15	0.1635167745527113
1107	16-17	0.1264674496492475
1107	18-19	0.12271176714418885
1107	20-21	0.1704405604775019
1107	22-23	0.16808436619326983
1107	24-25	0.1426946022920177
1107	26-27	0.1898384158212849
1107	28-29	0.15259725768056853
1107	30-31	0.18821117994033898
1107	32-33	0.15197456418670896
1107	34-35	0.16635011749654893
1107	36-37	0.1449033908855597
1107	38-39	0.17965668468299612
1107	40-41	0.15845353041480337
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2216	50-51	-0.46105787584539826
2216	52-53	-0.45813830119961807
2216	54-55	-0.41885419037217986
2216	56-57	-0.39753052080158824
2216	58-59	-0.3974984247397515
2216	60-61	-0.409275398747873
2216	62-63	-0.42543611721484353
2216	64-65	-0.46648329458834326
2216	66-67	-0.4433097121503877
2216	68-69	-0.4528799510218846
2216	70-71	-0.4656098951862546
2216	72-73	-0.5036325784663447
2216	74-75	-0.526254531260669
2216	76-77	-0.4971269814244792
2216	78-79	-0.5447395218487614
2216	80-81	-0.5045371998304304
2216	82-83	-0.5024142594575665
2216	84-85	-0.5748453967616207
2216	86-87	-0.5938223144479764
2216	88-89	-0.595208764764088
2216	90-91	-0.5834948090284797
2216	92-93	-0.6080538358220409
2216	94-95	-0.578056765167652
2216	96-97	-0.6140479780916266
2216	98-99	-0.47091594240401946
2216	100	0.08671369581776034
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	11.0
7	68.0
8	245.0
9	631.0
10	1458.0
11	2785.0
12	4468.0
13	6706.0
14	9742.0
15	14537.0
16	21991.0
17	31738.0
18	43607.0
19	56482.0
20	70583.0
21	87178.0
22	105349.0
23	124430.0
24	146794.0
25	173175.0
26	202949.0
27	237983.0
28	286105.0
29	352478.0
30	410537.0
31	509691.0
32	664278.0
33	964066.0
34	1428171.0
35	7026262.0
36	3040439.0
37	4414499.0
38	1777214.0
39	307410.0
40	488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.613716938057205	21.919669217832745	33.88136735823542	25.58524648587463
2	15.270717948168622	21.36431695812396	42.796487693624506	20.568477400082912
3	11.572960309791789	17.638813440340734	49.5954147448375	21.19281150502998
4	11.726769974218767	14.465926759026152	53.9960335675033	19.81126969925179
5	10.455475379454745	16.14426759433917	55.85549151021194	17.544765515994143
6	9.667131337897526	12.098013471662373	51.7395958092838	26.495259381156306
7	13.360747571937958	12.563120023540538	49.68675509049061	24.389377314030895
8	10.100042851026355	12.580922822513463	51.028224850505325	26.29080947595486
9	9.982619851017654	11.45793469418343	53.721366573038445	24.83807888176047
10-11	12.860322435770966	17.66142876651731	55.205309780245095	14.272939017466632
12-13	11.252005857929818	22.82494010534693	51.8915415921519	14.031512444571348
14-15	12.838942903148306	20.840814033683237	51.51640887491962	14.803834188248846
16-17	16.95402391342263	21.543166251982033	51.57952902625587	9.923280808339472
18-19	17.02813563735298	17.64064738703408	51.202748912048065	14.128468063564872
20-21	12.988744353963424	16.702771910444433	55.30857630175664	14.999907433835505
22-23	12.777595321340932	17.408672660684342	56.805031787191695	13.008700230783035
24-25	12.719001552950527	18.53973014485037	55.31898041540189	13.422287886797216
26-27	15.64792392054006	18.373171194789993	52.527437419667734	13.45146746500221
28-29	14.184596637236046	20.64677379515195	54.39958113571917	10.769048431892832
30-31	14.31889360853282	19.106146791673016	54.33488653367689	12.240073066117283
32-33	14.760304550045374	19.790767080004713	54.05093565078438	11.397992719165535
34-35	14.63875024163559	19.86544682538562	54.13131594587114	11.364486987107648
36-37	14.820883215330285	19.15878250023059	53.946878149795666	12.07345613464346
38-39	15.282735121571767	19.202291873731888	53.33890321847667	12.176069786219678
40-41	15.383220105056678	19.70018842114033	53.35907594236154	11.557515531441451
42-43	15.309609587363848	19.795907272328776	53.568434943891894	11.326048196415481
44-45	15.618648577587727	18.97289666107813	53.52933190040019	11.879122860933945
46-47	15.89882148151687	18.59144831709102	53.75167237697672	11.758057824415381
48-49	15.348546741356694	18.60809244181812	54.08146092689072	11.961899889934466
50-51	15.24170634673004	18.528583309463247	54.28164960818539	11.948060735621322
52-53	14.947136578838707	18.92664469286112	54.25610382233535	11.87011490596483
54-55	14.857254825111315	19.145770085897013	54.14087914567286	11.856095943318813
56-57	14.81442821937419	19.159911515654052	53.831502355826444	12.194157909145309
58-59	14.733925165215144	19.284491841666945	54.039069233106574	11.942513760011337
60-61	14.392142948262855	19.138900054850243	54.136038100425985	12.332918896460923
62-63	14.308350551543672	19.072522862174957	54.10297882513121	12.51614776115016
64-65	14.301301670126604	19.09657056199037	53.98516747897515	12.616960288907883
66-67	14.095355136865331	18.882886065370634	54.28235044896187	12.739408348802165
68-69	14.093870052001337	18.633676786198844	54.44259796185589	12.829855199943932
70-71	13.987875934992358	18.545160594821816	54.64068295001244	12.826280520173386
72-73	13.77230551746536	18.354299404239033	55.08671577582233	12.786679302473283
74-75	13.685501025463356	18.16659241531611	55.31415202919073	12.833754530029799
76-77	13.833996543262286	18.051870511273844	55.332627595480666	12.781505349983204
78-79	13.989269195551088	18.010137347210627	55.36305343739215	12.637540019846142
80-81	14.008187356626634	17.773860818933237	55.62017378347627	12.597778040963867
82-83	14.02378826330413	17.794927573457635	55.7392020201935	12.442082143044736
84-85	13.940002552988384	17.98601628362591	55.60995221471743	12.464028948668284
86-87	13.95106174301943	18.139007151397667	55.476020595954246	12.433910509628658
88-89	13.914580344087602	18.489782440631117	55.35645972783367	12.23917748744761
90-91	13.879644571566155	18.76434917064649	55.264439199652685	12.091567058134673
92-93	13.73098718824339	18.864596490328385	55.16023492530962	12.244181396118611
94-95	13.701028293972964	19.13249063143505	55.008174553380854	12.15830652121114
96-97	13.458089722448088	19.586237476403305	55.092830165692554	11.862842635456058
98-99	13.340319022451855	20.033016946754984	54.95193551855453	11.674728512238639
100	13.254210537705596	20.484400514007973	54.57055833784029	11.690830610446138
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1009174.0
1	2149395.5
2	2852310.0
3	1562987.0
4	426633.5
5	102776.5
6	51943.0
7	36866.5
8	32058.0
9	32206.5
10	36134.0
11	43922.0
12	57058.5
13	76320.0
14	101300.0
15	130818.5
16	164241.0
17	199940.5
18	234822.5
19	265518.5
20	286756.5
21	299949.0
22	310536.5
23	317448.5
24	321747.0
25	324181.5
26	322123.0
27	315211.5
28	302542.5
29	285814.0
30	267418.0
31	246821.0
32	224692.5
33	204097.0
34	184356.5
35	164208.5
36	145988.5
37	128735.5
38	112530.0
39	98499.0
40	85696.5
41	75419.5
42	68594.5
43	65416.5
44	69657.0
45	86280.0
46	118988.0
47	168540.5
48	237708.5
49	332757.0
50	456876.5
51	609801.5
52	777028.5
53	925379.5
54	1007988.5
55	986861.0
56	858228.5
57	658913.0
58	446236.0
59	268204.5
60	146062.0
61	73415.0
62	34958.5
63	16388.0
64	8009.5
65	4347.0
66	2781.5
67	2033.5
68	1526.5
69	1178.5
70	954.5
71	746.0
72	566.5
73	421.5
74	327.0
75	252.0
76	171.5
77	105.0
78	60.5
79	42.5
80	33.0
81	19.5
82	15.5
83	11.5
84	8.0
85	9.5
86	6.5
87	4.5
88	4.0
89	4.0
90	3.5
91	2.0
92	0.5
93	2.0
94	2.0
95	0.5
96	0.5
97	0.0
98	1.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017163496466166602
2	0.11617991180111584
3	0.0
4	1.1099001853444517E-4
5	0.037985223943228515
6	0.0016826086809821888
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.256567545772727E-4
14-15	8.768211464221169E-4
16-17	6.6594011120667106E-6
18-19	2.2198003706889035E-6
20-21	5.283124882239591E-4
22-23	6.459619078704709E-4
24-25	0.0028835206815248857
26-27	0.0023019329844043927
28-29	0.0034828667816108896
30-31	0.0037226052216452915
32-33	0.003966783262421071
34-35	0.004270895913205451
36-37	0.0033985143675247117
38-39	0.0019756223299131244
40-41	0.0035583399942143126
42-43	0.004925737022558677
44-45	0.004661580778446697
46-47	0.012040197210616613
48-49	0.005098881451472411
50-51	0.0070478661769372685
52-53	0.0027014970511283955
54-55	0.0011054605846030738
56-57	0.005513984120791237
58-59	0.002592726832964639
60-61	0.014317712390943428
62-63	0.01265286211292675
64-65	0.009827056241039776
66-67	0.011749403362056366
68-69	0.009529602991367462
70-71	0.008452999811583345
72-73	0.005562819728946392
74-75	0.0036737696134901353
76-77	5.838074974911816E-4
78-79	0.010144487694048288
80-81	0.006239858842006508
82-83	0.01042862214149647
84-85	0.008537352225669523
86-87	0.00744965004403196
88-89	0.009518503989514019
90-91	0.005556160327834326
92-93	0.00919219333502275
94-95	0.0030522255096972427
96-97	0.005620534538584304
98-99	0.44666379098927983
100	1.7326030249308444
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.2524548E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.942918721148914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.24190700826298	51.709365643001114
2	8.752740081539127	10.14318614263928
3	1.4436895042297277	2.509547508064767
4	0.2891358358078027	0.670134969343319
5	0.08122373413302315	0.23531701125489848
6	0.032865262270811615	0.11425855323041276
7	0.027070682693791145	0.10979880569366471
8	0.01659019175292221	0.07690273058462774
9	0.009996636221081297	0.052131085203870615
>10	0.08019162232608364	0.8770363113024155
>50	0.009735041996701761	0.39880523871158
>100	0.011122617538239416	1.3914116486385688
>500	0.0016451656304579377	0.6454529864956112
>1k	8.899960865843893E-4	1.077819293656015
>5k	3.22166791358388E-4	1.317003568305618
>10k+	8.744527194013386E-4	28.67182850387421
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	226307	1.0047127249789873	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181268	0.8047575471880724	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181074	0.803896264644245	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	176409	0.7831855271857177	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	161544	0.7171908621651364	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	159656	0.7088088959654152	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153222	0.6802445047953903	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	152316	0.6762222265237021	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	147251	0.6537356487686234	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146597	0.6508321498837624	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	135177	0.6001319094172278	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	135012	0.5993993752949005	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131839	0.5853125221425087	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	129639	0.5755454005114775	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	126172	0.5601533047411207	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123187	0.5469010965281079	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	119902	0.5323170080926819	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118923	0.5279706389668729	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114487	0.508276570078121	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	113022	0.5017725549920025	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	109329	0.48537710945409424	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	109172	0.4846800921376979	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106107	0.47107271586537497	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	103740	0.4605641809105337	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100404	0.4457536728372974	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100128	0.444528343032677	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97308	0.4320086689419916	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94475	0.4194312800416683	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94429	0.41922705840756497	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93012	0.4129361441570326	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89586	0.3977260720170722	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78574	0.34883718865301977	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74379	0.3302130635429399	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73711	0.32724741024769954	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71348	0.3167566336958238	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70306	0.3121305697233081	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67943	0.30163979317143236	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59055	0.26218062178206636	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54254	0.24086609862271155	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52138	0.2314719034539561	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49135	0.21813978242759854	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49133	0.21813090322611578	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48772	0.21652820735847841	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47974	0.21298540596685892	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47505	0.21090323321915272	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46594	0.20685875694375755	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46454	0.20623721283996466	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45460	0.20182424970303509	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43496	0.1931048738469691	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41477	0.1841413199501273	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40148	0.1782410905648362	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39590	0.1757637933511474	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39152	0.1738192482264239	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37600	0.16692898787580554	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37037	0.16442949265840984	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36556	0.1622940447018071	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36028	0.15994993551035963	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34786	0.1544359513895684	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34474	0.15305079595825852	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33136	0.147110610166295	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29250	0.12985832168530084	No Hit
TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29171	0.129507593226732	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28120	0.12484157284754394	No Hit
TCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27262	0.12103239541144178	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26859	0.11924323631266652	No Hit
CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26589	0.11804454411249451	No Hit
GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25906	0.11501229680613347	No Hit
GCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25826	0.11465712874682325	No Hit
CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25759	0.11435967549715094	No Hit
CATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25323	0.1124240095739102	No Hit
AACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25166	0.11172699225751388	No Hit
GTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24740	0.10983572234168695	No Hit
TGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24077	0.10689226705015346	No Hit
ACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23273	0.1033228280540857	No Hit
GCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22632	0.10047704397886253	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0020555351432579244	0.0	0.0	4.439600741377807E-6	0.0
2	0.005012309237015544	0.0	0.0	1.7758402965511228E-5	0.0
3	0.008417483005652322	0.0	0.0	2.2198003706889034E-5	0.0
4	0.01372724549234018	0.0	0.0	2.2198003706889034E-5	0.0
5	0.024107032025681492	0.0	0.0	2.2198003706889034E-5	0.0
6	0.04559469961395008	0.0	0.0	2.2198003706889034E-5	0.0
7	0.0757173906441985	0.0	0.0	2.2198003706889034E-5	0.0
8	0.2120442106096868	0.0	0.0	2.2198003706889034E-5	0.0
9	0.7360680445174749	0.0	0.0	2.2198003706889034E-5	0.0
10-11	1.689430127521316	0.0	0.0	2.2198003706889034E-5	0.0
12-13	2.5252049452890244	0.0	0.0	2.2198003706889034E-5	0.0
14-15	3.24932158461071	0.0	0.0	3.107720518964465E-5	0.0
16-17	3.818822468712802	0.0	2.2198003706889035E-6	5.327520889653368E-5	0.0
18-19	4.3632529274283325	0.0	4.439600741377807E-6	7.103361186204491E-5	0.0
20-21	5.050500902393247	0.0	4.439600741377807E-6	7.325341223273381E-5	0.0
22-23	5.92157498565565	0.0	4.439600741377807E-6	7.547321260342271E-5	0.0
24-25	7.032332013943187	0.0	4.439600741377807E-6	8.213261371548943E-5	0.0
26-27	8.303520674421524	2.2198003706889035E-6	4.439600741377807E-6	9.323161556893395E-5	0.0
28-29	9.771152788504345	4.439600741377807E-6	4.439600741377807E-6	9.767121631031175E-5	0.0
30-31	11.378365949896086	4.439600741377807E-6	4.439600741377807E-6	9.989101668100067E-5	0.0
32-33	13.233337690061528	4.439600741377807E-6	4.439600741377807E-6	1.0211081705168957E-4	0.0
34-35	15.094760170104191	4.439600741377807E-6	4.439600741377807E-6	1.0433061742237847E-4	0.0
36-37	16.455841866393946	4.439600741377807E-6	4.439600741377807E-6	1.220890203878897E-4	0.0
38-39	17.454188203909794	4.439600741377807E-6	4.439600741377807E-6	1.4206722372408983E-4	0.0
40-41	18.496058167293747	4.439600741377807E-6	4.439600741377807E-6	1.6870482817235666E-4	0.0
42-43	19.321244537293268	4.439600741377807E-6	4.439600741377807E-6	1.953424326206235E-4	0.0
44-45	19.944065026299306	4.439600741377807E-6	4.439600741377807E-6	2.1310083558613474E-4	0.0
46-47	20.459127526110624	4.439600741377807E-6	4.439600741377807E-6	2.2641963781026814E-4	0.0
48-49	20.916710515123324	4.439600741377807E-6	4.439600741377807E-6	2.6637604448266844E-4	0.0
50-51	21.369418822521986	4.439600741377807E-6	4.439600741377807E-6	3.240908541205799E-4	0.0
52-53	21.852931299664704	4.439600741377807E-6	4.439600741377807E-6	3.9734426635331375E-4	0.0
54-55	22.315033802232126	4.439600741377807E-6	4.439600741377807E-6	4.506194752498474E-4	0.0
56-57	22.77856585623827	4.439600741377807E-6	4.439600741377807E-6	4.8391648081018093E-4	0.0
58-59	23.22281672422461	4.439600741377807E-6	4.439600741377807E-6	5.016748837756922E-4	0.0
60-61	23.65791979488334	4.439600741377807E-6	4.439600741377807E-6	5.283124882239591E-4	0.0
62-63	24.08965542837974	4.439600741377807E-6	4.439600741377807E-6	5.505104919308481E-4	0.0
64-65	24.514911464594093	4.439600741377807E-6	4.439600741377807E-6	5.904668986032483E-4	0.0
66-67	24.91167636305066	8.879201482755614E-6	4.439600741377807E-6	6.459619078704709E-4	0.0
68-69	25.276285233337425	8.879201482755614E-6	4.439600741377807E-6	7.169955197325158E-4	0.0
70-71	25.62781282003972	8.879201482755614E-6	4.439600741377807E-6	7.414133238100938E-4	0.0
72-73	25.975189380048825	8.879201482755614E-6	4.439600741377807E-6	7.791499301118051E-4	0.0
74-75	26.31482993576608	8.879201482755614E-6	4.439600741377807E-6	7.991281334480052E-4	0.0
76-77	26.649151849795167	8.879201482755614E-6	4.439600741377807E-6	8.635023441979834E-4	0.0
78-79	26.96848789152173	8.879201482755614E-6	4.439600741377807E-6	0.0010699437786720516	0.0
80-81	27.281057981718433	8.879201482755614E-6	4.439600741377807E-6	0.001314121819447831	0.0
82-83	27.596746891435956	8.879201482755614E-6	4.439600741377807E-6	0.0013673970283443646	0.0
84-85	27.912415822950145	8.879201482755614E-6	4.439600741377807E-6	0.0013873752316805646	0.0
86-87	28.22502142995278	8.879201482755614E-6	4.439600741377807E-6	0.001402913834275387	0.0
88	28.458342427115518	8.879201482755614E-6	4.439600741377807E-6	0.0014073534350167649	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	5510	0.0	67.723335	1
GACTTTT	5065	0.0	57.786674	1
CACTTTT	8125	0.0	54.32456	1
TGCCCTA	11370	0.0	53.791264	6
CCTTTTC	10410	0.0	52.299725	1
ACTTTTC	10040	0.0	49.493362	1
CCCCTAC	72530	0.0	46.64389	7
AGCTTTT	3215	0.0	44.933903	1
CCCCCTA	38040	0.0	44.92452	6
CCCTTTT	11595	0.0	44.68207	1
GCATTTT	4335	0.0	44.396725	1
CCGCCTA	6520	0.0	44.380722	6
GCTTTTC	6480	0.0	44.224102	1
CATTTTT	39025	0.0	44.035667	1
CGCCCTA	12085	0.0	43.223442	6
ATGCCCT	3980	0.0	43.097233	5
ACCTTTT	9805	0.0	41.32114	1
CTCCCTA	29305	0.0	40.795033	6
GCTTTCT	5010	0.0	40.66932	1
ACTTTTT	47195	0.0	40.40082	1
>>END_MODULE