##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248482_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21358923
Sequences flagged as poor quality	0
Sequence length	100
%GC	26
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.308607414334514	31.0	30.0	33.0	22.0	34.0
2	29.86661733833677	31.0	30.0	34.0	23.0	34.0
3	30.3374577454116	31.0	30.0	34.0	26.0	34.0
4	33.88204971758174	35.0	35.0	37.0	28.0	37.0
5	33.682235991018835	35.0	35.0	37.0	28.0	37.0
6	33.39533678734644	35.0	33.0	37.0	27.0	37.0
7	33.53956704652196	35.0	33.0	37.0	28.0	37.0
8	33.56567487040428	35.0	33.0	37.0	28.0	37.0
9	34.79117219533963	37.0	34.0	39.0	27.0	39.0
10-11	35.53285266302987	38.0	35.0	39.0	30.0	39.0
12-13	35.86158311914885	39.0	35.0	39.0	31.0	39.0
14-15	36.792145582434095	39.0	36.0	41.0	30.5	41.0
16-17	36.49600204560876	39.0	36.0	40.5	30.0	41.0
18-19	35.8167803451513	38.0	34.5	40.0	30.0	41.0
20-21	35.242241240347184	37.0	34.0	39.5	28.0	41.0
22-23	34.78684646224906	36.5	34.0	39.0	27.5	40.0
24-25	34.448072873337296	35.0	33.0	39.0	26.5	40.0
26-27	34.17876531040446	35.0	33.0	39.0	25.0	40.0
28-29	34.03768048136135	35.0	33.0	39.0	25.0	40.0
30-31	33.891127914080684	35.0	33.0	39.0	25.0	40.0
32-33	33.754182362097566	35.0	33.0	39.0	25.0	40.0
34-35	33.61498538573316	35.0	33.0	38.0	25.0	40.0
36-37	33.310498474103774	35.0	31.5	38.0	25.0	40.0
38-39	33.076908746756565	35.0	31.0	38.0	24.5	40.0
40-41	32.82555536625138	35.0	30.5	38.0	24.0	40.0
42-43	32.62752948732481	35.0	30.0	38.0	23.0	40.0
44-45	32.31728804865301	35.0	30.0	37.5	23.0	40.0
46-47	32.27542622350387	35.0	30.0	37.0	23.0	39.0
48-49	32.346682344423456	35.0	30.0	37.0	23.0	39.0
50-51	32.24294167360405	35.0	30.0	37.0	23.0	39.0
52-53	32.165834110643125	35.0	30.0	37.0	23.0	39.0
54-55	32.01808775657837	35.0	29.5	37.0	22.0	39.0
56-57	32.3307105185032	35.0	30.0	37.0	23.5	39.0
58-59	32.49067261022478	35.0	30.0	36.5	24.5	39.0
60-61	32.354848463099	35.0	30.0	36.0	25.0	39.0
62-63	32.15287613050527	34.5	30.0	35.5	24.5	39.0
64-65	31.970130656868797	34.0	30.0	35.0	24.0	39.0
66-67	31.790683172555095	34.0	30.0	35.0	24.0	38.0
68-69	31.651288456819664	34.0	30.0	35.0	23.5	37.5
70-71	31.52429083151805	34.0	30.0	35.0	24.0	37.0
72-73	31.418049285537478	34.0	30.0	35.0	24.0	36.5
74-75	31.313224618113935	34.0	30.0	35.0	24.0	36.0
76-77	31.170583366960965	34.0	30.0	35.0	23.0	36.0
78-79	31.041076368878713	34.0	30.0	35.0	23.0	36.0
80-81	30.957209874299373	34.0	30.0	35.0	23.0	35.0
82-83	30.86250416278012	34.0	30.0	35.0	22.5	35.0
84-85	30.77622848773789	34.0	29.5	35.0	21.0	35.0
86-87	30.65270091099631	34.0	29.0	35.0	20.0	35.0
88-89	30.52308416955293	34.0	29.0	35.0	20.0	35.0
90-91	30.327698428427315	34.0	29.0	35.0	19.0	35.0
92-93	30.10908078089892	34.0	29.0	35.0	18.0	35.0
94-95	29.893256602872718	34.0	29.0	35.0	16.0	35.0
96-97	29.60633312363175	34.0	28.0	35.0	14.0	35.0
98-99	29.24918051813755	33.5	27.5	35.0	10.0	35.0
100	27.666228255048253	32.0	25.0	35.0	8.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3736376078159189
1101	2	0.3326737301888407
1101	3	0.3815968699395924
1101	4	0.3192531572602988
1101	5	0.2629909061955402
1101	6	0.22696782156678097
1101	7	0.2509541312946624
1101	8	0.28735431907289666
1101	9	0.3999635071018659
1101	10-11	0.4730856493571167
1101	12-13	0.5069487168602791
1101	14-15	0.6946914334479999
1101	16-17	0.7250958875197782
1101	18-19	0.7029222517569664
1101	20-21	0.586770701961747
1101	22-23	0.615966130700123
1101	24-25	0.6797340209586054
1101	26-27	0.6663448336580302
1101	28-29	0.6996825939508398
1101	30-31	0.6885480543713811
1101	32-33	0.7089264453219073
1101	34-35	0.7647639225920528
1101	36-37	0.7258964937088237
1101	38-39	0.710719105658633
1101	40-41	0.7086576988930844
1101	42-43	0.6878892343844569
1101	44-45	0.6415654049907289
1101	46-47	0.7077617381114578
1101	48-49	0.7235042386241801
1101	50-51	0.6997106609188322
1101	52-53	0.5980926229183581
1101	54-55	0.6232955814915329
1101	56-57	0.5623164950610899
1101	58-59	0.5435022123819948
1101	60-61	0.5562877738905243
1101	62-63	0.5085605241118003
1101	64-65	0.5562537499782003
1101	66-67	0.5305149987671882
1101	68-69	0.54071040162464
1101	70-71	0.5008080975877469
1101	72-73	0.5134463500896658
1101	74-75	0.5228007230329474
1101	76-77	0.49990052096865156
1101	78-79	0.49583023165385853
1101	80-81	0.49340406022513505
1101	82-83	0.510565769095443
1101	84-85	0.4667470907719107
1101	86-87	0.4534881758255018
1101	88-89	0.42731602744282426
1101	90-91	0.4196518426497562
1101	92-93	0.39229372902747883
1101	94-95	0.3964176840665168
1101	96-97	0.3696886118730909
1101	98-99	0.33109031965742375
1101	100	0.29908342267856014
1102	1	0.2519696991027125
1102	2	0.2868970048650681
1102	3	0.2759757787138426
1102	4	0.24246201309448878
1102	5	0.2050931678073482
1102	6	0.18071700389091205
1102	7	0.1754068745991546
1102	8	0.23951973643379176
1102	9	0.21845145722947024
1102	10-11	0.3049516685420315
1102	12-13	0.4296267161887073
1102	14-15	0.5042417147957678
1102	16-17	0.4891872426119406
1102	18-19	0.43868409110187656
1102	20-21	0.3683553452861901
1102	22-23	0.3646266110245122
1102	24-25	0.36265005816616736
1102	26-27	0.3289419541617775
1102	28-29	0.363483385735762
1102	30-31	0.3514116923643442
1102	32-33	0.3570495181437394
1102	34-35	0.43485755897926737
1102	36-37	0.35856421586891685
1102	38-39	0.37737206024866765
1102	40-41	0.38158229181586734
1102	42-43	0.4430496329720768
1102	44-45	0.34259173301681756
1102	46-47	0.4138580290582894
1102	48-49	0.4063707264322787
1102	50-51	0.44435972258839485
1102	52-53	0.4043657049681215
1102	54-55	0.41828783861095076
1102	56-57	0.4525017890136027
1102	58-59	0.4945285924437073
1102	60-61	0.5087810628825977
1102	62-63	0.5145049471644683
1102	64-65	0.493472068655894
1102	66-67	0.5113440107490064
1102	68-69	0.5132443553209249
1102	70-71	0.5419207927084209
1102	72-73	0.4384794833375203
1102	74-75	0.40430915263107536
1102	76-77	0.43226292124371923
1102	78-79	0.4527165506351025
1102	80-81	0.4571181799548363
1102	82-83	0.4803716997288632
1102	84-85	0.4578932057194187
1102	86-87	0.483834674432444
1102	88-89	0.4479189507107968
1102	90-91	0.4544064768980256
1102	92-93	0.42895802443216624
1102	94-95	0.40440685720541225
1102	96-97	0.39628781503758503
1102	98-99	0.3797661024116117
1102	100	0.3445917744697127
1103	1	0.22758430158432574
1103	2	0.21799955856701914
1103	3	0.22414184515912794
1103	4	0.20699381812372053
1103	5	0.1491175732465706
1103	6	0.1312369850143824
1103	7	0.14747482780417442
1103	8	0.1739816813906856
1103	9	0.1503234944914027
1103	10-11	0.21783363365243957
1103	12-13	0.28941488028222295
1103	14-15	0.33592600886597523
1103	16-17	0.3263833523026207
1103	18-19	0.3217744732837602
1103	20-21	0.2891997215247528
1103	22-23	0.20184750631906923
1103	24-25	0.2573579915964572
1103	26-27	0.22933232751208266
1103	28-29	0.2414828084560341
1103	30-31	0.24320985307706877
1103	32-33	0.26480805920774486
1103	34-35	0.2673406945810157
1103	36-37	0.22143511706455143
1103	38-39	0.19789519647855514
1103	40-41	0.20724315466313925
1103	42-43	0.22893455693980513
1103	44-45	0.15555059597693344
1103	46-47	0.19784824533738288
1103	48-49	0.22983812378961943
1103	50-51	0.26958671914270127
1103	52-53	0.29249226753844226
1103	54-55	0.2666924593612663
1103	56-57	0.2769617373878148
1103	58-59	0.27129986803351613
1103	60-61	0.27924672539776196
1103	62-63	0.2704308693858053
1103	64-65	0.225962269420382
1103	66-67	0.24125017219664358
1103	68-69	0.2569293839908191
1103	70-71	0.26646267109784105
1103	72-73	0.2771724112207572
1103	74-75	0.3033027435938678
1103	76-77	0.2932454036174974
1103	78-79	0.28250330854537964
1103	80-81	0.2744888714701581
1103	82-83	0.3069285953603966
1103	84-85	0.2559452864393279
1103	86-87	0.3088199489606325
1103	88-89	0.2865169380873631
1103	90-91	0.270466128193771
1103	92-93	0.29274381846089526
1103	94-95	0.276347733133381
1103	96-97	0.2771698829997611
1103	98-99	0.2566013766164552
1103	100	0.20657221465386755
1104	1	0.15044067516997828
1104	2	0.14382978256375978
1104	3	0.12397115587279472
1104	4	0.11876109313833894
1104	5	0.08095178213050502
1104	6	0.06886222901562178
1104	7	0.0960441015830753
1104	8	0.1389361135218934
1104	9	0.07084669836130786
1104	10-11	0.11556832074831647
1104	12-13	0.20431014416766402
1104	14-15	0.24780393392217803
1104	16-17	0.20338156512637795
1104	18-19	0.20257178050552938
1104	20-21	0.16282521999127653
1104	22-23	0.1869949699932434
1104	24-25	0.17702185940122206
1104	26-27	0.18674906419066417
1104	28-29	0.12714518730730617
1104	30-31	0.13951629314687608
1104	32-33	0.18679255391778327
1104	34-35	0.23201495770354086
1104	36-37	0.2104354996406883
1104	38-39	0.14011866455766864
1104	40-41	0.08009045908585932
1104	42-43	0.11209272225868716
1104	44-45	0.1615889901065728
1104	46-47	0.20626078266197823
1104	48-49	0.24894292010832686
1104	50-51	0.2598098436641507
1104	52-53	0.28055228502136487
1104	54-55	0.3046660437739348
1104	56-57	0.2572178098357014
1104	58-59	0.24089161375511026
1104	60-61	0.25796709367646997
1104	62-63	0.2769658364194427
1104	64-65	0.28043968586665713
1104	66-67	0.2677926738889056
1104	68-69	0.287260844810028
1104	70-71	0.30668870385306946
1104	72-73	0.26476001733718135
1104	74-75	0.2607811276013692
1104	76-77	0.2508857623346472
1104	78-79	0.25357339590087946
1104	80-81	0.2484627377573574
1104	82-83	0.2989640857938909
1104	84-85	0.29189045904306
1104	86-87	0.29872853662452314
1104	88-89	0.31951799484613375
1104	90-91	0.3076584761353871
1104	92-93	0.3012636881885342
1104	94-95	0.28924732148094634
1104	96-97	0.31296890386489196
1104	98-99	0.2521487846200863
1104	100	0.24820298232916826
1105	1	0.08115501634425826
1105	2	0.08890272157304224
1105	3	0.05420260240954278
1105	4	0.07879449461960064
1105	5	0.02720494896654202
1105	6	-0.020000318046058396
1105	7	0.024144160789091984
1105	8	0.050101442307287414
1105	9	0.06728171099750568
1105	10-11	0.15061327707217487
1105	12-13	0.21087511984966056
1105	14-15	0.21543464327383077
1105	16-17	0.2216559020788864
1105	18-19	0.18446808788708324
1105	20-21	0.1609685976481785
1105	22-23	0.1196880251048924
1105	24-25	0.1177985239025503
1105	26-27	0.06667115394947132
1105	28-29	0.07629709711574151
1105	30-31	0.07221919279518119
1105	32-33	0.08263819855378074
1105	34-35	0.14740199692583644
1105	36-37	0.18561360820854844
1105	38-39	0.1218009041537158
1105	40-41	0.09460420440382933
1105	42-43	0.11405338405008081
1105	44-45	0.11918570020998231
1105	46-47	0.07256514128097535
1105	48-49	0.17894011400668575
1105	50-51	0.1754903256212188
1105	52-53	0.20046695257544656
1105	54-55	0.22545405000208518
1105	56-57	0.21984842308197727
1105	58-59	0.19815869883201742
1105	60-61	0.25007735493177563
1105	62-63	0.2570956657222965
1105	64-65	0.23814726520924623
1105	66-67	0.20438817182303382
1105	68-69	0.2255804583824279
1105	70-71	0.24634246824516381
1105	72-73	0.22059617200438097
1105	74-75	0.2123508533698022
1105	76-77	0.1962310381870438
1105	78-79	0.21387710462767018
1105	80-81	0.1964777561085036
1105	82-83	0.17868274993196565
1105	84-85	0.166664052730642
1105	86-87	0.15943664024011284
1105	88-89	0.1882874458531063
1105	90-91	0.16889390275521166
1105	92-93	0.16050120330328
1105	94-95	0.161638498296778
1105	96-97	0.13802923983711324
1105	98-99	0.1056437534314476
1105	100	0.12304369217519096
1106	1	-0.036029866619109896
1106	2	-0.024600285692674362
1106	3	-0.015311359344273967
1106	4	-0.016350503200918354
1106	5	-0.07343438397753488
1106	6	-0.046980912041775014
1106	7	-0.03165515466353952
1106	8	-0.06040743928475223
1106	9	-0.0809007171440399
1106	10-11	-0.00971546300036863
1106	12-13	0.07073865169212468
1106	14-15	0.05982345233562825
1106	16-17	0.03397375656753354
1106	18-19	-0.021133668752426615
1106	20-21	-0.019706356091013788
1106	22-23	-0.03152433114252773
1106	24-25	0.014699435119453597
1106	26-27	-0.007852766566188052
1106	28-29	-0.014196975531518774
1106	30-31	-0.0214646450836824
1106	32-33	0.021229572984609035
1106	34-35	0.11557445850034753
1106	36-37	0.08920950572513675
1106	38-39	0.08554985629773171
1106	40-41	0.08040599162716688
1106	42-43	0.05852487610700052
1106	44-45	0.060220656969491415
1106	46-47	0.03815588958835292
1106	48-49	0.0777909891110582
1106	50-51	0.09818704250556465
1106	52-53	0.08900864321692126
1106	54-55	0.12257064546098206
1106	56-57	0.12059319806055413
1106	58-59	0.11153937845063666
1106	60-61	0.10502830240545791
1106	62-63	0.11734000019438184
1106	64-65	0.13062626564510893
1106	66-67	0.1583084446921248
1106	68-69	0.11152543998173314
1106	70-71	0.12535507053952344
1106	72-73	0.09567185811317458
1106	74-75	0.08819612201412141
1106	76-77	0.06438421766883096
1106	78-79	0.10232694891627148
1106	80-81	0.11746561718034698
1106	82-83	0.08943440852099727
1106	84-85	0.10364796323106162
1106	86-87	0.13011924988736467
1106	88-89	0.09716918173389288
1106	90-91	0.08552892366719433
1106	92-93	0.0379315001748779
1106	94-95	0.02361534323539516
1106	96-97	0.05132958255468978
1106	98-99	0.0328450853179838
1106	100	0.05146796823203914
1107	1	0.003838741209115426
1107	2	0.013176603796161146
1107	3	0.05670084258474617
1107	4	0.025795925755488724
1107	5	0.03989861745500889
1107	6	-0.016488341273614537
1107	7	0.0020889590538217817
1107	8	0.0037197205124996913
1107	9	-0.035675363938871385
1107	10-11	0.0744821803032707
1107	12-13	0.12295441938818641
1107	14-15	0.204719216531835
1107	16-17	0.16256354173129495
1107	18-19	0.13107611617746073
1107	20-21	0.1474945627662123
1107	22-23	0.11348881628406815
1107	24-25	0.14457642315566943
1107	26-27	0.11150268076706737
1107	28-29	0.17354897607609132
1107	30-31	0.14014148766697332
1107	32-33	0.17198337727273127
1107	34-35	0.21746279961040216
1107	36-37	0.21421302274318776
1107	38-39	0.1945903244530811
1107	40-41	0.18231377840995577
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2216	6	0.40145162505916687
2216	7	0.25747201485287974
2216	8	0.19001773270030498
2216	9	0.30401876053043253
2216	10-11	0.18198999224450318
2216	12-13	-0.01089955979304591
2216	14-15	0.04985153051432434
2216	16-17	0.06046318356528246
2216	18-19	0.18892060976057934
2216	20-21	0.23258043645418525
2216	22-23	0.29453893065772974
2216	24-25	0.4175379143245408
2216	26-27	0.46636012897275947
2216	28-29	0.47965877772539045
2216	30-31	0.47866627262205697
2216	32-33	0.47658659506895873
2216	34-35	0.4455348914935797
2216	36-37	0.5495530005667391
2216	38-39	0.5399424472573529
2216	40-41	0.5819872755344093
2216	42-43	0.5796368025558678
2216	44-45	0.6148771313020731
2216	46-47	0.5562961900057246
2216	48-49	0.5182090064009373
2216	50-51	0.4955265697424309
2216	52-53	0.5290989176577909
2216	54-55	0.5203728705144783
2216	56-57	0.47979501562895166
2216	58-59	0.3771446378503498
2216	60-61	0.4122160220394093
2216	62-63	0.3821061906267076
2216	64-65	0.37933150655853254
2216	66-67	0.35493296709899624
2216	68-69	0.3812173573328401
2216	70-71	0.3097780961275305
2216	72-73	0.2630183327761024
2216	74-75	0.24244152084682113
2216	76-77	0.1645595141993894
2216	78-79	0.14557411830110212
2216	80-81	0.09882648200186495
2216	82-83	0.02624602949061483
2216	84-85	0.011190214844447155
2216	86-87	0.003745608914830001
2216	88-89	-0.020709191829734408
2216	90-91	-0.04085004281033022
2216	92-93	-0.009188004341062594
2216	94-95	-0.006603840862272392
2216	96-97	0.012171558465649213
2216	98-99	0.09643031223832921
2216	100	0.49433087252643304
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	368.0
6	3529.0
7	11374.0
8	20181.0
9	32531.0
10	45904.0
11	55195.0
12	57865.0
13	61432.0
14	68125.0
15	77221.0
16	90508.0
17	105547.0
18	124664.0
19	142230.0
20	161898.0
21	184237.0
22	205446.0
23	236653.0
24	323762.0
25	412568.0
26	341805.0
27	690307.0
28	462589.0
29	795718.0
30	685021.0
31	847113.0
32	1137977.0
33	2000728.0
34	2415454.0
35	5912015.0
36	2365111.0
37	1090919.0
38	186773.0
39	6143.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.589822753547333	34.15290958748008	25.753423618394418	25.503844040578173
2	11.841998257116147	37.90255965523991	26.882525011993856	23.372917075650083
3	10.295467613231247	40.89124718507576	24.389511587264955	24.423773614428033
4	8.906321106123343	40.96668277234991	24.2372086229079	25.889787498618844
5	8.789928630293232	39.94095301251627	25.35004809699666	25.919070260193845
6	8.834660921815614	37.14013056638573	26.57054791211022	27.454660599688435
7	11.683332211237333	42.34445712823891	21.953305699495452	24.018904961028312
8	10.620896006788358	39.13196840496124	19.310144991861247	30.93699059638915
9	8.660371124517843	38.28133562726922	20.30376718901042	32.75452605920252
10-11	10.717707679477133	54.062837989082105	19.53535401205579	15.684100319384978
12-13	10.50859694805687	54.81871576166526	19.21178166477261	15.460905625505264
14-15	11.461692550178885	53.42556136605765	18.95937442280911	16.153371660954356
16-17	11.40999239636759	53.16847864216415	19.610934014511674	15.810594946956583
18-19	11.628044058199762	51.91235291337429	20.59879812543581	15.860804902990136
20-21	11.000925211352728	51.43711614222144	21.27039901650382	16.291559629922013
22-23	11.240780924773423	52.07347098628523	21.74337802624034	14.942370062701007
24-25	11.33352557149066	52.09081703230074	21.278682918609707	15.296974477598893
26-27	12.522103759632449	52.263700281142455	20.459142532608034	14.755053426617062
28-29	12.159569094378027	52.99833704161956	20.991432948187512	13.850660915814903
30-31	12.445062641884833	52.92373820115594	20.80134428447345	13.829854872485775
32-33	12.822321229026933	53.070063699635284	20.263549454423323	13.844065616914456
34-35	12.904583856905305	53.19186749911313	20.11871543330597	13.784833210675593
36-37	13.322168112290697	53.04170028167904	19.814267453901913	13.821864152128358
38-39	13.652410791447425	53.07182391394979	19.757034863102813	13.518730431499975
40-41	13.826845859490538	53.22549517595297	19.793373782142854	13.15428518241363
42-43	13.976575230118463	53.27719329754241	19.98891270070945	12.75731877162968
44-45	14.115819242236121	52.80141451654369	20.01058391951202	13.072182321708173
46-47	14.151144784665673	52.44772546545631	20.33699835690664	13.06413139297138
48-49	14.056540196517432	52.548395231248925	20.637722323757664	12.757342248475986
50-51	14.29351096026705	52.492157493147005	20.764736124569577	12.449595422016364
52-53	14.428069774274697	52.617682363703736	20.84816058090248	12.10608728111909
54-55	14.395578009690011	52.893326373204	20.72673938096284	11.984356236143153
56-57	14.221547683607835	53.1229567845043	20.415150254014776	12.240345277873093
58-59	13.975987612417903	53.136689409976	20.586351514262805	12.300971463343298
60-61	13.930548054133093	52.97446330599611	20.866325026293474	12.22866361357732
62-63	13.977526100344608	52.41215878249713	21.13989621780239	12.470418899355867
64-65	13.80893946477075	52.30493773434334	21.219281811761242	12.666840989124667
66-67	13.530504051057388	52.0826993265748	21.488374290247965	12.89842233211985
68-69	13.425009727796336	51.93903973599385	21.344271663466515	13.291678872743287
70-71	13.29882887054005	51.83906545611705	21.466960453692515	13.39514521965039
72-73	13.437761645680546	51.997537408353345	21.398232644073293	13.166468301892817
74-75	13.416152566662424	52.18388661148236	21.258624119551023	13.141336702304201
76-77	13.011930073441496	52.62891999623107	21.260848114978167	13.098301815349267
78-79	12.639243060200252	53.13647839564689	21.053538763371314	13.17073978078154
80-81	12.253109076579804	53.854670652994216	20.771597093134016	13.120623177291959
82-83	11.789136902278125	54.60034522981857	20.82233680046536	12.78818106743794
84-85	11.328811907476416	55.550985219218596	20.71581533169382	12.404387541611168
86-87	10.921617077348401	56.2785271079159	20.526726618071795	12.273129196663907
88-89	10.624418443831775	56.99090560158272	20.346092228148212	12.03858372643729
90-91	10.527205481410132	57.65340571738462	20.01353595867382	11.805852842531424
92-93	10.517263541748186	58.0878604133963	19.583880698661527	11.810995346193993
94-95	10.572955981394513	58.32691305355966	19.230720520779478	11.86941044426635
96-97	10.718016952712786	58.17360596864112	19.065164092239513	12.043212986406587
98-99	10.963698312857971	57.760886568663224	18.893624944648217	12.381790173830586
100	11.394728665968872	56.81343689023654	18.83189757125577	12.959936872538824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2488928.0
1	2348997.0
2	1350884.5
3	297667.0
4	98752.0
5	92634.5
6	81870.5
7	63621.0
8	47844.0
9	41139.5
10	43039.5
11	51575.0
12	66350.0
13	87808.5
14	116083.0
15	150595.0
16	189765.0
17	232119.5
18	276911.5
19	321174.0
20	363946.0
21	405297.5
22	439811.5
23	467902.5
24	489910.0
25	501143.0
26	502120.0
27	492385.5
28	473766.5
29	448077.5
30	416758.5
31	384882.0
32	352939.5
33	320567.5
34	288843.0
35	259310.5
36	233646.5
37	211266.5
38	192545.0
39	178046.5
40	168365.0
41	165416.5
42	172795.0
43	193376.5
44	228746.0
45	278774.0
46	337280.5
47	392844.0
48	435024.5
49	457908.0
50	463820.0
51	457626.5
52	444649.5
53	429196.0
54	410559.5
55	379062.0
56	329326.0
57	267906.5
58	202354.5
59	142025.5
60	93651.0
61	59475.0
62	37765.5
63	25031.0
64	18084.5
65	14082.5
66	11569.5
67	9907.5
68	8602.5
69	7594.0
70	6723.5
71	5993.5
72	5470.0
73	5031.0
74	4587.0
75	4257.0
76	4036.5
77	3755.5
78	3478.0
79	3193.5
80	2987.0
81	2846.0
82	2703.5
83	2595.5
84	2493.5
85	2413.5
86	2339.5
87	2262.5
88	2226.0
89	2179.5
90	2095.0
91	1993.0
92	1904.5
93	1857.0
94	1817.5
95	1721.5
96	1632.0
97	1477.5
98	1296.5
99	1102.0
100	827.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014087788977000386
2	3.2773188048854335E-4
3	0.0
4	1.4045652020937572E-5
5	4.681884006979191E-6
6	0.004209013722274293
7	3.979601405932312E-4
8	0.0
9	0.0
10-11	2.8091304041875145E-4
12-13	2.8091304041875145E-5
14-15	4.120057926141688E-4
16-17	2.457989103664075E-4
18-19	1.8025253426869886E-4
20-21	9.129673813609423E-5
22-23	7.256920210817746E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	2.3877608435593874E-4
32-33	4.4477898066302315E-5
34-35	1.4982028822333411E-4
36-37	1.8025253426869886E-4
38-39	6.484409349666179E-4
40-41	0.0018914811388195932
42-43	0.0011704710017447976
44-45	4.962797047397942E-4
46-47	5.828945588689093E-4
48-49	9.691499894446925E-4
50-51	0.0
52-53	9.129673813609423E-5
54-55	5.828945588689093E-4
56-57	5.899173848793781E-4
58-59	5.337347767956277E-4
60-61	5.15007240767711E-5
62-63	2.692083304013035E-4
64-65	1.7791159226520926E-4
66-67	2.575036203838555E-4
68-69	1.2172898418145896E-4
70-71	6.554637609770867E-4
72-73	5.805536168654197E-4
74-75	3.558231845304185E-4
76-77	4.0030108259672085E-4
78-79	8.591257152806814E-4
80-81	0.0011704710017447976
82-83	5.03302530750263E-4
84-85	8.146478172143792E-4
86-87	0.0013624282460309445
88-89	0.0013156094059611526
90-91	0.0012102670158041207
92-93	7.888974551759936E-4
94-95	7.98261223189952E-4
96-97	7.420786151062017E-4
98-99	3.7923260456531444E-4
100	0.0010815152056121932
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.1358923E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.8314898489157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.74025120123575	62.22877918093598
2	7.137367644225784	9.682765618145611
3	0.8257032743122882	1.680260498091913
4	0.1239044757517817	0.336185007567688
5	0.044643069428563734	0.15141029553840296
6	0.02393648539571747	0.0974188479682997
7	0.018474060546991713	0.08771861353230655
8	0.012232359651073426	0.06637913436000582
9	0.010780105392073594	0.0658107548525413
>10	0.054311708128586	0.6754755627613304
>50	0.00381722445905849	0.1841341394792483
>100	0.00285777772398852	0.40176450335047764
>500	4.998789758327276E-4	0.24584689819853747
>1k	6.381706232658101E-4	0.9437479934819473
>5k	1.8031747476275853E-4	0.9455331447417968
>10k+	4.022466744707684E-4	22.20676980699381
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1173207	5.492819090176035	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	332922	1.558702187371526	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	272365	1.2751813375608874	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	257020	1.2033378274737916	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	178734	0.8368118561034187	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	149394	0.6994453793386493	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	144744	0.6776746187061959	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	142919	0.6691301803934591	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	116604	0.5459264027498015	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	110801	0.5187574298573013	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	109687	0.5135418110735265	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	106608	0.49912629021603755	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105178	0.49243119608605734	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	97985	0.45875440442385607	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	84873	0.3973655413243449	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	80235	0.3756509632999754	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78313	0.3666523822385614	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	75848	0.35511153816135765	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68468	0.3205592341898512	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60041	0.2811049976630376	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56294	0.26356197828888656	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53998	0.25281237260886236	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51809	0.2425637285175849	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47324	0.22156547874628324	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42793	0.20035186231066052	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42776	0.20027227028254188	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42519	0.1990690260927482	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40417	0.18922770591007795	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31877	0.14924441649047565	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31413	0.1470720223112373	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31021	0.14523672378050148	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29714	0.1391175013833797	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29324	0.13729156662065778	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26293	0.12310077619550387	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24845	0.11632140815339799	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23900	0.11189702776680266	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23149	0.10838093287756129	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22159	0.10374586771065189	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21723	0.10170456628360897	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0013202912899681319	0.0	0.0	4.681884006979191E-6	0.0
2	0.003768916625618249	0.0	0.0	2.3409420034895953E-5	0.0
3	0.0063018158733939905	0.0	0.0	4.213695606281272E-5	0.0
4	0.01071215060796839	0.0	0.0	4.6818840069791906E-5	0.0
5	0.0173463802458579	0.0	0.0	7.022826010468786E-5	0.0
6	0.0294069134478363	0.0	0.0	8.427391212562543E-5	0.0
7	0.045681142256095965	0.0	0.0	8.427391212562543E-5	0.0
8	0.10235534816057908	0.0	0.0	8.427391212562543E-5	0.0
9	0.30957085242547105	0.0	0.0	8.895579613260463E-5	0.0
10-11	0.7358048905368497	0.0	0.0	9.129673813609423E-5	0.0
12-13	1.2644387547068736	0.0	0.0	9.363768013958381E-5	0.0
14-15	1.8328030865601228	0.0	0.0	9.8319564146563E-5	0.0
16-17	2.423277615636332	0.0	0.0	1.1704710017447977E-4	0.0
18-19	3.0585132967612645	0.0	0.0	1.3109275219541736E-4	0.0
20-21	3.8849009381231436	0.0	0.0	1.3577463620239653E-4	0.0
22-23	4.842290034942305	2.3409420034895955E-6	0.0	1.3577463620239653E-4	0.0
24-25	5.863294230706295	4.681884006979191E-6	0.0	1.3577463620239653E-4	0.0
26-27	6.950174875390487	4.681884006979191E-6	0.0	1.3577463620239653E-4	0.0
28-29	8.192962257507086	4.681884006979191E-6	0.0	1.4045652020937572E-4	0.0
30-31	9.6382856008236	4.681884006979191E-6	0.0	1.4513840421635492E-4	0.0
32-33	11.277422555435027	4.681884006979191E-6	0.0	1.545021722303133E-4	0.0
34-35	12.79604547476481	4.681884006979191E-6	0.0	1.6386594024427167E-4	0.0
36-37	13.971825732973521	4.681884006979191E-6	0.0	1.6620688224776127E-4	0.0
38-39	14.850072262538706	4.681884006979191E-6	0.0	2.060028963070844E-4	0.0
40-41	15.62370911679395	7.022826010468786E-6	0.0	2.2473043233500116E-4	0.0
42-43	16.224701498291836	9.363768013958382E-6	0.0	2.3877608435593874E-4	0.0
44-45	16.69047170590015	9.363768013958382E-6	0.0	2.551626783803659E-4	0.0
46-47	17.07735450893287	9.363768013958382E-6	0.0	2.785720984152619E-4	0.0
48-49	17.43339306012761	9.363768013958382E-6	0.0	3.019815184501578E-4	0.0
50-51	17.802161653937326	9.363768013958382E-6	0.0	3.4177753250948093E-4	0.0
52-53	18.203991371662326	9.363768013958382E-6	0.0	4.16687676621148E-4	0.0
54-55	18.63096514744681	9.363768013958382E-6	0.0	4.8223405271885663E-4	0.0
56-57	19.089075792819706	9.363768013958382E-6	0.0	5.03302530750263E-4	0.0
58-59	19.58735700297248	9.363768013958382E-6	0.0	5.196891247746902E-4	0.0
60-61	20.110744816112685	9.363768013958382E-6	0.0	5.360757187991173E-4	0.0
62-63	20.59826237493342	9.363768013958382E-6	0.0	5.524623128235445E-4	0.0
64-65	21.052051641367875	9.363768013958382E-6	0.0	5.875764428758884E-4	0.0
66-67	21.458928430052396	1.1704710017447978E-5	0.0	6.578047029805763E-4	0.0
68-69	21.812867156270002	1.4045652020937572E-5	0.0	6.99941659043389E-4	0.0
70-71	22.130184185785023	1.4045652020937572E-5	0.0	7.23351079078285E-4	0.0
72-73	22.4291599347027	1.4045652020937572E-5	0.0	7.397376731027121E-4	0.0
74-75	22.715810623972004	1.4045652020937572E-5	0.0	7.748518031550561E-4	0.0
76-77	22.997468083947865	1.4045652020937572E-5	0.0	8.286934692353168E-4	0.0
78-79	23.27143086755826	1.4045652020937572E-5	0.0	9.129673813609422E-4	0.0
80-81	23.538970106311073	1.4045652020937572E-5	0.0	0.0010042641194970363	0.0
82-83	23.8068511225964	1.4045652020937572E-5	0.0	0.0010253325975284429	0.0
84-85	24.082583658361425	1.4045652020937572E-5	0.0	0.001053423901570318	0.0
86-87	24.362829530309185	1.4045652020937572E-5	0.0	0.0010815152056121932	0.0
88	24.574422596120602	1.4045652020937572E-5	0.0	0.0010861970896191722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTGCT	1200	0.0	43.082867	7
TTGCTGA	4875	0.0	42.420055	9
CTTGCTG	3510	0.0	35.751747	8
GCCTGCT	1410	0.0	34.99962	7
TACCCGA	10445	0.0	34.334328	6
TGAAAAA	21275	0.0	32.657307	1
ACCCGAC	11665	0.0	32.47457	7
CTGCTGA	6010	0.0	31.984678	9
CCTGCTG	4820	0.0	31.885548	8
CTACCCG	10765	0.0	31.392603	5
CCCGACG	12860	0.0	31.357357	8
TCGCGAC	660	0.0	29.910097	6
GTCACGC	425	0.0	29.859827	2
GACACGC	1000	0.0	29.14098	2
GCTGCTG	1125	0.0	28.40858	8
ACTTGCC	1305	0.0	28.092701	3
TGCCAAA	2765	0.0	27.537996	6
TTGCGCC	845	0.0	27.255163	3
TGACGCC	770	0.0	26.857857	3
TGCCCAA	2460	0.0	26.748867	5
>>END_MODULE