##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248481_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20725654 Sequences flagged as poor quality 0 Sequence length 100 %GC 32 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.52437853107072 31.0 30.0 33.0 23.0 34.0 2 30.057910790173377 31.0 30.0 34.0 25.0 34.0 3 30.49892920146211 31.0 31.0 34.0 26.0 34.0 4 33.97957970349211 35.0 35.0 37.0 30.0 37.0 5 33.7583297492084 35.0 35.0 37.0 28.0 37.0 6 33.4840924682039 35.0 33.0 37.0 27.0 37.0 7 33.65476539365175 35.0 35.0 37.0 28.0 37.0 8 33.66717725771163 35.0 35.0 37.0 28.0 37.0 9 34.89392971628302 37.0 34.0 39.0 27.0 39.0 10-11 35.50463083094989 38.0 35.0 39.0 30.0 39.0 12-13 35.776126123691924 39.0 35.0 39.0 30.0 39.0 14-15 36.68542592190336 39.0 36.0 41.0 30.5 41.0 16-17 36.362656927496715 39.0 36.0 40.5 30.0 41.0 18-19 35.73327386918647 38.0 34.5 40.0 29.5 41.0 20-21 35.2029636796986 37.0 34.0 39.5 27.5 41.0 22-23 34.796806532618945 36.5 34.0 39.0 27.0 41.0 24-25 34.48747402615136 36.0 33.5 39.0 26.0 41.0 26-27 34.23913407026866 35.0 33.0 39.0 25.0 40.0 28-29 34.07562275236285 35.0 33.0 39.0 25.0 40.0 30-31 33.941849699893666 35.0 33.0 39.0 25.0 40.0 32-33 33.78739676924067 35.0 33.0 39.0 25.0 40.0 34-35 33.60572402202604 35.0 33.0 39.0 25.0 40.0 36-37 33.326011980128584 35.0 31.5 38.0 24.0 40.0 38-39 33.07718021829371 35.0 31.0 38.0 23.5 40.0 40-41 32.807676563547766 35.0 30.5 38.0 23.0 40.0 42-43 32.565234563888794 35.0 30.0 38.0 23.0 40.0 44-45 32.24527375589692 35.0 30.0 37.5 22.0 40.0 46-47 32.157151494471535 35.0 30.0 37.0 22.0 39.0 48-49 32.1973277417446 35.0 30.0 37.0 22.0 39.0 50-51 32.061604521623295 35.0 30.0 37.0 22.0 39.0 52-53 31.948748348303027 35.0 29.5 37.0 22.0 39.0 54-55 31.73779961298206 34.5 29.0 36.0 22.0 39.0 56-57 31.992504868603906 34.5 30.0 36.0 23.5 39.0 58-59 32.114584755684916 35.0 30.0 36.0 24.0 39.0 60-61 31.95034419661739 34.0 30.0 35.0 24.0 39.0 62-63 31.739509739958024 34.0 30.0 35.0 23.5 38.0 64-65 31.560391990525368 34.0 30.0 35.0 23.0 37.0 66-67 31.39372885410516 34.0 30.0 35.0 23.0 37.0 68-69 31.26249579386011 34.0 30.0 35.0 23.0 36.0 70-71 31.16044953273851 34.0 30.0 35.0 23.0 36.0 72-73 31.0807511068167 34.0 30.0 35.0 23.0 35.5 74-75 30.995720400427412 34.0 30.0 35.0 23.0 35.0 76-77 30.857933505982487 34.0 29.0 35.0 22.0 35.0 78-79 30.720916116808667 34.0 29.0 35.0 20.5 35.0 80-81 30.627561402887455 34.0 29.0 35.0 20.0 35.0 82-83 30.524345311371114 34.0 29.0 35.0 20.0 35.0 84-85 30.429621424732844 34.0 29.0 35.0 20.0 35.0 86-87 30.280608491292966 34.0 29.0 35.0 19.0 35.0 88-89 30.12007958832083 34.0 29.0 35.0 18.0 35.0 90-91 29.87858301118025 34.0 29.0 35.0 16.0 35.0 92-93 29.61283972028096 34.0 28.0 35.0 13.5 35.0 94-95 29.358614280639827 34.0 28.0 35.0 10.0 35.0 96-97 29.035603556828654 33.0 27.0 35.0 10.0 35.0 98-99 28.645085554356932 33.0 26.5 35.0 9.0 35.0 100 27.016628715310986 32.0 23.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33398099424120886 1101 2 0.35006046007365654 1101 3 0.35418600658553956 1101 4 0.23461884440203562 1101 5 0.22946868190463476 1101 6 0.12995881916645402 1101 7 0.1823197888190009 1101 8 0.21494078596460042 1101 9 0.24497919134634571 1101 10-11 0.41321751625727643 1101 12-13 0.49820111894774755 1101 14-15 0.6703283903547259 1101 16-17 0.7138761728456515 1101 18-19 0.7013213315886659 1101 20-21 0.6275158576372917 1101 22-23 0.6218743767951764 1101 24-25 0.6318820637177325 1101 26-27 0.648269213252668 1101 28-29 0.7144633236571281 1101 30-31 0.6762024562488165 1101 32-33 0.6914577937467854 1101 34-35 0.740972680805946 1101 36-37 0.7232111702156487 1101 38-39 0.6391497529369872 1101 40-41 0.6831375802413575 1101 42-43 0.7243122512143358 1101 44-45 0.6448323728127434 1101 46-47 0.7173135318296389 1101 48-49 0.729145083447726 1101 50-51 0.7021918383004433 1101 52-53 0.6473237201527837 1101 54-55 0.657453331955729 1101 56-57 0.589424917186733 1101 58-59 0.5445627546156047 1101 60-61 0.5296729968145542 1101 62-63 0.4858863158671447 1101 64-65 0.5333530462601921 1101 66-67 0.5134997321036607 1101 68-69 0.5312586884992925 1101 70-71 0.5104346370317216 1101 72-73 0.506873825641101 1101 74-75 0.5075442581103253 1101 76-77 0.514818566810586 1101 78-79 0.494738707772175 1101 80-81 0.49251869404676185 1101 82-83 0.4753530819787777 1101 84-85 0.4994118662224807 1101 86-87 0.4661104797118192 1101 88-89 0.45191878158657417 1101 90-91 0.42844157370558733 1101 92-93 0.40922367252145264 1101 94-95 0.3818599924390007 1101 96-97 0.3136857298306488 1101 98-99 0.3591714484984472 1101 100 0.38215961223676587 1102 1 0.23005522118089416 1102 2 0.2522071092343303 1102 3 0.24339356064156092 1102 4 0.19646164333960314 1102 5 0.15954778762291255 1102 6 0.05082789521086539 1102 7 0.087876349977833 1102 8 0.1946388343860761 1102 9 0.14410201084026397 1102 10-11 0.2696956848636205 1102 12-13 0.3811820949315674 1102 14-15 0.4619951218749776 1102 16-17 0.464437987144791 1102 18-19 0.4669520975475123 1102 20-21 0.40560727368399085 1102 22-23 0.3896225440664409 1102 24-25 0.3585622165753932 1102 26-27 0.4156859664094199 1102 28-29 0.3862099055937094 1102 30-31 0.37942290968271664 1102 32-33 0.3783389211525474 1102 34-35 0.42204095687020526 1102 36-37 0.3551202405708267 1102 38-39 0.3428015911671025 1102 40-41 0.33112388987338903 1102 42-43 0.3942235382202952 1102 44-45 0.35678856712392104 1102 46-47 0.3785643461334729 1102 48-49 0.3536891001343321 1102 50-51 0.4534716455108665 1102 52-53 0.4228971611341166 1102 54-55 0.376645270834139 1102 56-57 0.3920379184070981 1102 58-59 0.47202345166639503 1102 60-61 0.39408448408391195 1102 62-63 0.3994082177052114 1102 64-65 0.4348189642481337 1102 66-67 0.43114000872879643 1102 68-69 0.44741909578874584 1102 70-71 0.4594094293381161 1102 72-73 0.42671084411162497 1102 74-75 0.39474618600610967 1102 76-77 0.3852366476767415 1102 78-79 0.424407028652503 1102 80-81 0.41174030008538764 1102 82-83 0.48273371728001635 1102 84-85 0.4472525404224541 1102 86-87 0.442617865975965 1102 88-89 0.40543655739488926 1102 90-91 0.38382242302365555 1102 92-93 0.38652091841336755 1102 94-95 0.40954167025041954 1102 96-97 0.38862738173194344 1102 98-99 0.3315474814315458 1102 100 0.2855476730497486 1103 1 0.2925173968606174 1103 2 0.2515625576443128 1103 3 0.262964511272461 1103 4 0.24552378942971131 1103 5 0.16768281262537954 1103 6 0.10525367290755128 1103 7 0.1616326809061448 1103 8 0.23190805329687691 1103 9 0.17318894168100485 1103 10-11 0.2509361596877966 1103 12-13 0.34642334438115086 1103 14-15 0.414883993432845 1103 16-17 0.4107507369169454 1103 18-19 0.40768685792498616 1103 20-21 0.3676982371445874 1103 22-23 0.29807721091162875 1103 24-25 0.31226814038301853 1103 26-27 0.26511050806846725 1103 28-29 0.3148078994732444 1103 30-31 0.27374827406598 1103 32-33 0.32707703778003605 1103 34-35 0.3655364455838068 1103 36-37 0.3018946215387146 1103 38-39 0.27843162464803584 1103 40-41 0.22872663816856686 1103 42-43 0.2678649615684563 1103 44-45 0.23157597806176256 1103 46-47 0.28987497581873356 1103 48-49 0.31128915863592965 1103 50-51 0.3095430874824032 1103 52-53 0.3233544084745361 1103 54-55 0.3425994040498779 1103 56-57 0.3656210616395157 1103 58-59 0.3549718964738062 1103 60-61 0.36559067374629706 1103 62-63 0.3984866035730654 1103 64-65 0.3673148272866307 1103 66-67 0.3823635084269412 1103 68-69 0.4035871350427911 1103 70-71 0.4096666744950781 1103 72-73 0.4127201039785575 1103 74-75 0.42076401280599285 1103 76-77 0.43105154407608026 1103 78-79 0.43417150387672265 1103 80-81 0.40241200121073817 1103 82-83 0.4546186684025706 1103 84-85 0.43076818345127066 1103 86-87 0.43508138561133336 1103 88-89 0.4117599722268146 1103 90-91 0.36210065584872453 1103 92-93 0.3468724142636077 1103 94-95 0.3166841877742357 1103 96-97 0.37000844548476053 1103 98-99 0.34806586394642025 1103 100 0.23792915743181808 1104 1 0.12415495210192162 1104 2 0.13108382114153017 1104 3 0.16314450932995683 1104 4 0.10158404044565117 1104 5 0.09367494422971134 1104 6 0.09736870755727978 1104 7 0.14515933376739554 1104 8 0.13248862789355087 1104 9 0.09281284485319219 1104 10-11 0.15331069994420687 1104 12-13 0.2126375747809277 1104 14-15 0.28400652323250597 1104 16-17 0.25366213869850895 1104 18-19 0.2658916975364747 1104 20-21 0.23649505966741202 1104 22-23 0.24264360706150256 1104 24-25 0.2266098015690048 1104 26-27 0.21339192832706289 1104 28-29 0.1790564678087847 1104 30-31 0.224060037745204 1104 32-33 0.24844740636028462 1104 34-35 0.2571387236148581 1104 36-37 0.250819385511754 1104 38-39 0.1928879055081012 1104 40-41 0.1757214474266604 1104 42-43 0.2346666038497247 1104 44-45 0.2791943192640147 1104 46-47 0.31480164047441406 1104 48-49 0.31993593936617515 1104 50-51 0.27846859861950435 1104 52-53 0.32983282373346157 1104 54-55 0.3580619702899881 1104 56-57 0.3064586842111794 1104 58-59 0.3205984138641824 1104 60-61 0.34946536626337377 1104 62-63 0.32905130888211787 1104 64-65 0.29577016220765984 1104 66-67 0.2939551763615533 1104 68-69 0.3236200448068658 1104 70-71 0.3153359590790785 1104 72-73 0.3257967986874206 1104 74-75 0.33419550344138926 1104 76-77 0.31065387517901755 1104 78-79 0.3313309750692248 1104 80-81 0.31115236240463773 1104 82-83 0.3369146115249215 1104 84-85 0.3352241595148726 1104 86-87 0.3501041232137041 1104 88-89 0.3591917990860374 1104 90-91 0.33024648264551715 1104 92-93 0.3566205206200266 1104 94-95 0.32715348017227086 1104 96-97 0.34375755176924017 1104 98-99 0.3242574232365314 1104 100 0.34553493594481566 1105 1 0.1394057732853291 1105 2 0.19870669291241327 1105 3 0.1851498257474482 1105 4 0.1721907152579476 1105 5 0.044673462530703034 1105 6 0.06658796587184668 1105 7 0.11163010750494351 1105 8 0.10666894891360812 1105 9 0.07147887429918143 1105 10-11 0.14771656753755025 1105 12-13 0.20920919295640772 1105 14-15 0.2448327490627804 1105 16-17 0.3084278547590671 1105 18-19 0.23627976236805637 1105 20-21 0.2432813883701641 1105 22-23 0.2255978372574674 1105 24-25 0.21665941222954643 1105 26-27 0.20290778196362425 1105 28-29 0.18057108280986967 1105 30-31 0.16932494986852475 1105 32-33 0.16277502202119365 1105 34-35 0.21479017683979862 1105 36-37 0.21326405939629467 1105 38-39 0.2054669423097124 1105 40-41 0.20327214192280962 1105 42-43 0.20628540898078285 1105 44-45 0.17859793396802104 1105 46-47 0.16262819649245586 1105 48-49 0.1831958351879166 1105 50-51 0.22425002159217655 1105 52-53 0.25684982894710373 1105 54-55 0.2688520003018269 1105 56-57 0.26908220729341537 1105 58-59 0.2591464099709597 1105 60-61 0.26653843766523266 1105 62-63 0.2713256287510113 1105 64-65 0.2669908455884702 1105 66-67 0.2373432904245547 1105 68-69 0.24633997759322313 1105 70-71 0.28978673931137067 1105 72-73 0.28570450989665375 1105 74-75 0.26639893339130793 1105 76-77 0.2157645189729429 1105 78-79 0.22737145626435762 1105 80-81 0.23226811302148676 1105 82-83 0.2541297385740684 1105 84-85 0.24774804806423134 1105 86-87 0.195196591670026 1105 88-89 0.26375765694210074 1105 90-91 0.22259533219258643 1105 92-93 0.22444818969625757 1105 94-95 0.23695069310340244 1105 96-97 0.19612594565656494 1105 98-99 0.20227740016688855 1105 100 0.22822092186256882 1106 1 -0.0315961543277794 1106 2 0.08019175209791385 1106 3 0.09447806320569896 1106 4 0.07492596270692786 1106 5 0.0069210710295593 1106 6 -0.010010627685083762 1106 7 0.05431703405816535 1106 8 0.05313994566472502 1106 9 -0.0021950599416982186 1106 10-11 0.07293289914688472 1106 12-13 0.1200693798125343 1106 14-15 0.16615055246317922 1106 16-17 0.125488337916984 1106 18-19 0.04545793557704769 1106 20-21 0.07409633967589713 1106 22-23 0.03621133772345786 1106 24-25 0.0728063572964075 1106 26-27 0.05385803770713693 1106 28-29 0.05292368788918367 1106 30-31 0.08029119419221331 1106 32-33 0.022327022755234793 1106 34-35 0.1515732655299331 1106 36-37 0.12752921303920317 1106 38-39 0.1180955203307974 1106 40-41 0.11650670482418235 1106 42-43 0.06777500274679227 1106 44-45 0.0844864254737061 1106 46-47 0.1078506774827801 1106 48-49 0.1295089081854499 1106 50-51 0.1973441872202102 1106 52-53 0.21604880937850623 1106 54-55 0.18943835149328692 1106 56-57 0.19273614716344412 1106 58-59 0.188818459335657 1106 60-61 0.19618092218033212 1106 62-63 0.17754425865316747 1106 64-65 0.19857542263793349 1106 66-67 0.1666661416665569 1106 68-69 0.18012275898237107 1106 70-71 0.18482761681924842 1106 72-73 0.14803252476716722 1106 74-75 0.15541011678841699 1106 76-77 0.13354283161555003 1106 78-79 0.1661789407296297 1106 80-81 0.15381538027039454 1106 82-83 0.08630618015713054 1106 84-85 0.09735824717103014 1106 86-87 0.12482632965991058 1106 88-89 0.12518068037364216 1106 90-91 0.10503176922305713 1106 92-93 0.07293753490853305 1106 94-95 0.1024146806855839 1106 96-97 0.06674560614763081 1106 98-99 0.08192124606021522 1106 100 0.130269138982662 1107 1 -0.013515835808536991 1107 2 0.017683670193243017 1107 3 0.08639191389106671 1107 4 0.013525524262007593 1107 5 -0.004775339289295744 1107 6 -0.020432402750905965 1107 7 0.04642009944183201 1107 8 0.038691247613684254 1107 9 -0.028450675191351138 1107 10-11 0.10569647620878442 1107 12-13 0.1327664467736014 1107 14-15 0.19288866263553928 1107 16-17 0.1715093838840076 1107 18-19 0.14769818909769583 1107 20-21 0.1700520104711174 1107 22-23 0.13832903638631677 1107 24-25 0.19904930533359533 1107 26-27 0.16221377158429817 1107 28-29 0.20483944779198993 1107 30-31 0.17096544465812258 1107 32-33 0.18217260581650407 1107 34-35 0.2323940271243714 1107 36-37 0.27425120023034566 1107 38-39 0.23568140077333766 1107 40-41 0.2425170682611295 1107 42-43 0.3260045738459496 1107 44-45 0.24227537655666964 1107 46-47 0.19580429154265744 1107 48-49 0.21740331545662173 1107 50-51 0.3026847672509305 1107 52-53 0.27929511979603205 1107 54-55 0.27175758159547314 1107 56-57 0.2615365979018094 1107 58-59 0.25957612730987734 1107 60-61 0.25654376864832784 1107 62-63 0.23100841953515427 1107 64-65 0.19105321568882871 1107 66-67 0.21234257808432488 1107 68-69 0.27331977377625094 1107 70-71 0.2633570966762804 1107 72-73 0.25357190243881433 1107 74-75 0.24457871193114755 1107 76-77 0.2162890922996077 1107 78-79 0.15620208941675529 1107 80-81 0.23634751540802768 1107 82-83 0.2561968796203864 1107 84-85 0.17214273320202977 1107 86-87 0.21080420583031412 1107 88-89 0.2337147012518841 1107 90-91 0.2133774581592185 1107 92-93 0.12331898942874986 1107 94-95 0.14407936123775755 1107 96-97 0.11623387946519514 1107 98-99 0.13756297025858544 1107 100 0.16912369325998355 1108 1 0.06638420685325386 1108 2 0.07216341264608062 1108 3 0.1324228682511155 1108 4 0.12140393987643705 1108 5 0.121787874420896 1108 6 0.2006719936895891 1108 7 0.14434548618135778 1108 8 0.10581279485423778 1108 9 0.15922735825766665 1108 10-11 0.21253274406846856 1108 12-13 0.2049769323506183 1108 14-15 0.20911439391048248 1108 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2214 52-53 -0.4656686284409268 2214 54-55 -0.49179873621348946 2214 56-57 -0.461110244150845 2214 58-59 -0.47064541472565935 2214 60-61 -0.5007595946919849 2214 62-63 -0.48641978256899066 2214 64-65 -0.5228956435412861 2214 66-67 -0.47712565567281473 2214 68-69 -0.44750344333733594 2214 70-71 -0.47357375771576926 2214 72-73 -0.4819410349866544 2214 74-75 -0.48107061282057373 2214 76-77 -0.501241195494412 2214 78-79 -0.4750018849791928 2214 80-81 -0.4633977075985847 2214 82-83 -0.5404119329358572 2214 84-85 -0.491788834579971 2214 86-87 -0.4012540690325288 2214 88-89 -0.4083704589480597 2214 90-91 -0.4568800747211874 2214 92-93 -0.44102957342159144 2214 94-95 -0.39598676894500073 2214 96-97 -0.44403250107483316 2214 98-99 -0.4077908384595652 2214 100 -0.14784085965179727 2215 1 -0.23626166227727552 2215 2 -0.28876379260595186 2215 3 -0.3556949387140165 2215 4 -0.29226944173115754 2215 5 -0.22733625057338003 2215 6 -0.14180253679687382 2215 7 -0.18280387422575473 2215 8 -0.25579062912264305 2215 9 -0.2392358863897286 2215 10-11 -0.3682591148605425 2215 12-13 -0.47767817287881087 2215 14-15 -0.5541266944391836 2215 16-17 -0.5210678195111171 2215 18-19 -0.4567148585870413 2215 20-21 -0.48947338454828326 2215 22-23 -0.49766423508421553 2215 24-25 -0.5014439958581889 2215 26-27 -0.46262745237978464 2215 28-29 -0.4662321223539223 2215 30-31 -0.4019908525337854 2215 32-33 -0.5436439253742265 2215 34-35 -0.49983261104048893 2215 36-37 -0.4843307326655477 2215 38-39 -0.40499028475698395 2215 40-41 -0.3359519983911099 2215 42-43 -0.30535745412807813 2215 44-45 -0.3204542983888423 2215 46-47 -0.42752541196493254 2215 48-49 -0.4780643100670261 2215 50-51 -0.4585251904140364 2215 52-53 -0.4705639084009867 2215 54-55 -0.48158163174950275 2215 56-57 -0.4730302774438968 2215 58-59 -0.458074828099825 2215 60-61 -0.4918080113328749 2215 62-63 -0.48557558870195194 2215 64-65 -0.4152888364080134 2215 66-67 -0.4121845935951356 2215 68-69 -0.5287910912749041 2215 70-71 -0.5286035932539406 2215 72-73 -0.5166473261306983 2215 74-75 -0.5432029096488193 2215 76-77 -0.5205194319905395 2215 78-79 -0.48890552154066214 2215 80-81 -0.48575037741663607 2215 82-83 -0.527138873243711 2215 84-85 -0.5443376073898918 2215 86-87 -0.5226848666771957 2215 88-89 -0.4957401906450194 2215 90-91 -0.41918316504039055 2215 92-93 -0.3879339370529209 2215 94-95 -0.3764440583222637 2215 96-97 -0.41542662964753063 2215 98-99 -0.3571953316095531 2215 100 -0.08044401497671672 2216 1 0.5511862800256004 2216 2 0.3151136784500892 2216 3 0.09813349455368936 2216 4 0.16840938367441538 2216 5 0.2445103884494202 2216 6 0.3015344555265713 2216 7 0.18905431768101977 2216 8 0.14449057868640125 2216 9 0.18952531305068732 2216 10-11 0.108028924739763 2216 12-13 -0.05688815740484188 2216 14-15 -0.012855668563339862 2216 16-17 -0.03453868753657474 2216 18-19 0.055063028706413775 2216 20-21 0.06816789131563183 2216 22-23 0.11476420155534584 2216 24-25 0.20538625417751177 2216 26-27 0.2557639426335072 2216 28-29 0.2603884049053491 2216 30-31 0.22218239756657 2216 32-33 0.3052554833699901 2216 34-35 0.2658514470375479 2216 36-37 0.31024300839901997 2216 38-39 0.3309219382212163 2216 40-41 0.33103111382163064 2216 42-43 0.37213196227861545 2216 44-45 0.4016372931893173 2216 46-47 0.34822887904940103 2216 48-49 0.3064511363064497 2216 50-51 0.2721539609689998 2216 52-53 0.3199489871712373 2216 54-55 0.3449006741730898 2216 56-57 0.30719663665601615 2216 58-59 0.21708512343529662 2216 60-61 0.23952386525495228 2216 62-63 0.20240296627575916 2216 64-65 0.17986273214676984 2216 66-67 0.16827014823410735 2216 68-69 0.18742876112486329 2216 70-71 0.13647251214431577 2216 72-73 0.1030755981519178 2216 74-75 0.08707841255352733 2216 76-77 0.038895376003068804 2216 78-79 0.0047757342358067945 2216 80-81 -0.04040302885711711 2216 82-83 -0.14737473962467718 2216 84-85 -0.16077999445387547 2216 86-87 -0.16878360676303572 2216 88-89 -0.2061440707851041 2216 90-91 -0.2512174994940146 2216 92-93 -0.18373328481551 2216 94-95 -0.1535000610131263 2216 96-97 -0.1547407639064211 2216 98-99 -0.08307508454906554 2216 100 0.3242511770159595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 612.0 6 4617.0 7 12847.0 8 25674.0 9 44238.0 10 62530.0 11 73711.0 12 75377.0 13 75362.0 14 78646.0 15 86097.0 16 95008.0 17 106770.0 18 123246.0 19 138234.0 20 155883.0 21 174886.0 22 195721.0 23 226867.0 24 299613.0 25 371667.0 26 340071.0 27 617254.0 28 481013.0 29 781479.0 30 730087.0 31 898602.0 32 1179932.0 33 1954228.0 34 2465710.0 35 5679605.0 36 2234067.0 37 793639.0 38 136465.0 39 5879.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.8735938714239 32.051146811038016 25.65154629265387 26.423713024884215 2 13.389843225097495 34.91211703414742 27.22550761559088 24.472532125164197 3 11.789268507522127 37.71845752129221 24.314147095189373 26.178126875996288 4 10.549903648373087 36.99862908997446 24.379000146147416 28.072467115505045 5 10.636857166182274 36.404495260269734 25.216716945744338 27.74193062780365 6 10.594331591075207 33.39680792525757 26.417155307633916 29.591705176033305 7 14.320116887372865 37.89280904497203 21.75365605961943 26.033418008035667 8 12.583583610920071 35.407215617900405 19.390027451003476 32.619173320176046 9 10.257611171160148 34.60540738545573 20.519318714864195 34.61766272851993 10-11 14.249092884514821 48.16222807885867 19.77511757689264 17.813561459733876 12-13 14.044735819281726 49.7620714118444 18.554458951507506 17.63873381736637 14-15 15.698417503838675 48.126092204080464 17.860279853694802 18.315210438386053 16-17 16.01524991699887 47.921041786878185 18.58582910495179 17.47787919117116 18-19 16.46986994258699 45.975019512085915 19.790861704665378 17.76424884066172 20-21 15.277112907560083 45.51884657074892 21.03174085259271 18.172299669098287 22-23 15.523142406803553 46.322908927913815 21.732918533164895 16.421030132117735 24-25 15.603073369843962 46.29300238245799 21.02099649062944 17.082927757068607 26-27 17.497995479418886 46.44340053153449 19.749856868207875 16.308747120838742 28-29 16.717839157210673 47.53945762097543 20.779764054731398 14.962939167082496 30-31 17.046412461794265 47.3899669775846 20.54786638700634 15.01575417361479 32-33 17.618733733772736 47.82474730164669 19.63258377017328 14.923935194407298 34-35 17.685148053233494 47.960884096370215 19.29628554471656 15.057682305679732 36-37 18.190385748040335 47.5532030948931 18.958864308423575 15.297546848642988 38-39 18.558931358880177 47.54523357079884 18.92481091924474 14.971024151076238 40-41 18.683829654350895 47.77481585787725 18.95938904805027 14.581965439721584 42-43 18.738298499896818 47.85920357496856 19.313893007150003 14.088604917984615 44-45 18.97960891611411 47.14045152039004 19.323833739832327 14.556105823663525 46-47 19.020093395985896 46.502620248393335 19.76398078581011 14.713305569810661 48-49 18.70520271096712 46.59479205392213 20.285137424069784 14.414867811040969 50-51 18.873445441094404 46.468589121482005 20.599308953049203 14.05865648437439 52-53 18.879439075166307 46.58719646096145 20.733573393598814 13.799791070273429 54-55 18.786903999963332 46.831140670953964 20.61402186140643 13.767933467676274 56-57 18.50717807143674 47.055805941583316 20.171522081943966 14.265493905035973 58-59 18.030103850779962 47.059843441100696 20.41581565405162 14.494237054067721 60-61 17.809062418918394 46.88682223317752 20.803777059317447 14.500338288586642 62-63 17.853066378913184 46.303258473375244 21.07712822259204 14.766546925119528 64-65 17.64122333514695 46.137207327520066 21.113186936912214 15.108382400420775 66-67 17.30320659195784 45.863069077119924 21.498524193584963 15.33520013733728 68-69 17.14755105429108 45.8545391593106 21.26903192775825 15.728877858640072 70-71 16.858360686285298 45.822376330669236 21.475007105370405 15.844255877675062 72-73 16.933566549719924 46.14850510099126 21.410945307286585 15.506983042002231 74-75 16.903508122465226 46.55858618339319 21.198754765459206 15.339150928682383 76-77 16.4443848508463 47.18364279249875 21.195663790525007 15.17630856612994 78-79 15.992861828085045 47.838644176759374 20.98588864636127 15.182605348794306 80-81 15.53615609114774 48.80224674722053 20.640491929021106 15.021105232610626 82-83 15.079609256650969 49.672133318771614 20.59454953080068 14.653707893776739 84-85 14.631020642243334 50.79330982896487 20.405226235939487 14.1704432928523 86-87 14.250832270807349 51.68876433626015 20.040507830606014 14.019895562326482 88-89 13.986273321104703 52.450310202600846 19.781833953413653 13.781582522880795 90-91 13.934717350653775 53.14544244349268 19.40908541517745 13.510754790676085 92-93 13.993483147991984 53.62919514959773 18.89013761913315 13.487184083277132 94-95 14.129492680358766 53.825092837913445 18.444350459326596 13.601064022401193 96-97 14.344387645432441 53.5634165362437 18.294718534645 13.797477283678857 98-99 14.639892390033104 53.10063195066177 18.133332226810726 14.126143432494406 100 15.180626612810851 52.04649720233016 17.983772810468594 14.7891033743904 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2162326.0 1 2002593.0 2 1118493.0 3 240070.0 4 81529.0 5 74323.5 6 64177.5 7 49187.0 8 36621.5 9 30676.0 10 31478.5 11 37054.0 12 46733.0 13 61358.0 14 81221.5 15 106196.0 16 134575.0 17 164294.0 18 193874.5 19 222671.5 20 249300.0 21 271541.5 22 290069.5 23 304672.5 24 314745.5 25 319159.0 26 318660.5 27 312586.5 28 300767.0 29 285333.5 30 266589.0 31 246096.0 32 226330.0 33 207455.0 34 189848.0 35 174534.5 36 161774.0 37 152098.5 38 144377.0 39 139509.0 40 141182.0 41 150226.5 42 168942.5 43 202751.0 44 252724.5 45 317400.0 46 389118.0 47 456713.5 48 512408.0 49 553240.5 50 585029.0 51 618934.0 52 663207.0 53 711919.0 54 746144.0 55 742712.5 56 685499.0 57 581264.0 58 451521.5 59 320927.0 60 211054.0 61 130975.0 62 79560.0 63 49735.0 64 33435.5 65 24808.5 66 19764.5 67 16488.0 68 14258.5 69 12489.5 70 10913.5 71 9544.5 72 8596.0 73 7825.5 74 7132.5 75 6620.0 76 6191.0 77 5861.0 78 5610.5 79 5326.5 80 4935.5 81 4705.5 82 4550.5 83 4392.0 84 4325.0 85 4262.0 86 4093.5 87 3935.0 88 3777.0 89 3698.0 90 3601.0 91 3463.0 92 3398.5 93 3265.5 94 3101.5 95 2927.0 96 2757.0 97 2526.5 98 2213.0 99 1871.5 100 1456.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013500177123481845 2 3.811701189260421E-4 3 0.0 4 2.4124691071268487E-5 5 9.649876428507395E-6 6 0.0039853989649735536 7 2.9914616928372926E-4 8 0.0 9 0.0 10-11 3.280957985692514E-4 12-13 1.2062345535634243E-5 14-15 3.5945789696190046E-4 16-17 1.59222961070372E-4 18-19 1.8334765214164049E-4 20-21 6.513666589242491E-5 22-23 5.5486789463917515E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.6887283749887942E-4 32-33 2.1712221964141638E-5 34-35 8.443641874943971E-5 36-37 1.3027333178484982E-4 38-39 5.693427092819363E-4 40-41 0.0017273278807028237 42-43 0.0010277118396360375 44-45 4.849062905324966E-4 46-47 6.658414735670103E-4 48-49 0.0010084120867790227 50-51 0.0 52-53 1.2062345535634244E-4 54-55 4.2941950106857905E-4 56-57 4.873187596396234E-4 58-59 4.8008135231824287E-4 60-61 3.136209839264903E-5 62-63 2.0988481232003583E-4 64-65 1.6163543017749885E-4 66-67 1.736977757131331E-4 68-69 1.0132370249932765E-4 70-71 6.441292516028685E-4 72-73 5.958798694603316E-4 74-75 3.8840752624742266E-4 76-77 4.1976962464007167E-4 78-79 8.660764094585387E-4 80-81 0.001085611098207082 82-83 3.8840752624742266E-4 84-85 6.996160410667862E-4 86-87 0.0013437452926696547 88-89 0.0011941722080277902 90-91 0.0011531602332066336 92-93 7.551028305307037E-4 94-95 7.261532012451814E-4 96-97 6.610165353527565E-4 98-99 3.112085148193635E-4 100 9.263881371367099E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.0725654E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.08341422867484 #Duplication Level Percentage of deduplicated Percentage of total 1 93.27135375681229 67.23317628521568 2 5.861110731016552 8.449777453279946 3 0.6128497200252893 1.3252890068553098 4 0.105058504578537 0.30291902815119265 5 0.03650951512170782 0.1315865250903066 6 0.025893660161219034 0.11199020587786122 7 0.01558360264106885 0.07863234990458659 8 0.010885948007669781 0.06277570396069428 9 0.00746604888698287 0.04843604651147307 >10 0.04571711873754117 0.5918205157494142 >50 0.0035472592810626984 0.1804066187127336 >100 0.002458378259569687 0.36586066929821687 >500 4.56110127066567E-4 0.23047413448332915 >1k 5.749591195597107E-4 0.8387441531748171 >5k 1.6040616721404806E-4 0.7485257483764335 >10k+ 3.7428105683277787E-4 19.299585555357982 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1044074 5.037592541108714 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 295348 1.425035851703401 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 245924 1.1865681054021262 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 224586 1.0836135737863808 No Hit AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 149114 0.7194658368802258 No Hit ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 128271 0.618899649680536 No Hit AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 123981 0.5982006647413877 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 118172 0.570172598654788 No Hit AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94572 0.4563040567984007 No Hit AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 91702 0.44245648412349253 No Hit AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89368 0.43119507833142445 No Hit AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 88998 0.4294098511921506 No Hit CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86300 0.4163921678900941 No Hit ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80313 0.3875052628013572 No Hit AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 74422 0.35908155178118867 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64511 0.31126158913972024 No Hit AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 63678 0.30724241560724697 No Hit AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 62653 0.3022968539376369 No Hit TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57618 0.27800329002886953 No Hit CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46374 0.223751684747801 No Hit AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44048 0.21252887846144689 No Hit TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43277 0.20880885109825728 No Hit GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40588 0.19583459224012906 No Hit AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40226 0.1940879646065692 No Hit AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33692 0.16256181831463556 No Hit CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33498 0.16162578030107036 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33228 0.16032304698322183 No Hit AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32297 0.15583102950575167 No Hit AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27192 0.13119971992198654 No Hit AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26522 0.12796701131843657 No Hit AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26376 0.12726257033915553 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25466 0.12287187656418465 No Hit TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24351 0.11749207045529178 No Hit AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23808 0.11487212900495203 No Hit AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21282 0.10268433507574719 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002393169354269834 0.0 0.0 4.8249382142536975E-6 0.0 2 0.005968448571031824 0.0 0.0 2.8949629285522183E-5 0.0 3 0.010199919384932317 0.0 0.0 7.719901142805916E-5 4.8249382142536975E-6 4 0.016486813878104883 0.0 0.0 8.684888785656655E-5 4.8249382142536975E-6 5 0.027357399674818463 0.0 0.0 1.1579851714208873E-4 4.8249382142536975E-6 6 0.048852499419318685 0.0 0.0 1.1579851714208873E-4 4.8249382142536975E-6 7 0.07823154820590945 0.0 0.0 1.1579851714208873E-4 4.8249382142536975E-6 8 0.18649833679554817 0.0 0.0 1.1579851714208873E-4 4.8249382142536975E-6 9 0.5816077022225692 0.0 0.0 1.2062345535634244E-4 4.8249382142536975E-6 10-11 1.4361500968799343 0.0 0.0 1.2062345535634244E-4 4.8249382142536975E-6 12-13 2.5100028206588805 0.0 0.0 1.2303592446346928E-4 4.8249382142536975E-6 14-15 3.6207446095549023 2.4124691071268487E-6 0.0 1.3027333178484982E-4 4.8249382142536975E-6 16-17 4.6861078545458685 4.8249382142536975E-6 0.0 1.5922296107037204E-4 4.8249382142536975E-6 18-19 5.7549522924584195 4.8249382142536975E-6 0.0 1.736977757131331E-4 4.8249382142536975E-6 20-21 7.098757896855751 4.8249382142536975E-6 0.0 1.785227139273868E-4 4.8249382142536975E-6 22-23 8.641326830989264 4.8249382142536975E-6 2.4124691071268487E-6 1.785227139273868E-4 4.8249382142536975E-6 24-25 10.310193347819084 4.8249382142536975E-6 4.8249382142536975E-6 1.785227139273868E-4 4.8249382142536975E-6 26-27 12.069660141967052 4.8249382142536975E-6 4.8249382142536975E-6 1.785227139273868E-4 4.8249382142536975E-6 28-29 14.013335839727905 7.237407321380547E-6 4.8249382142536975E-6 1.785227139273868E-4 4.8249382142536975E-6 30-31 16.151056077651397 9.649876428507395E-6 4.8249382142536975E-6 1.833476521416405E-4 4.8249382142536975E-6 32-33 18.570323522722127 9.649876428507395E-6 4.8249382142536975E-6 1.929975285701479E-4 4.8249382142536975E-6 34-35 20.827526118114292 9.649876428507395E-6 4.8249382142536975E-6 1.929975285701479E-4 4.8249382142536975E-6 36-37 22.555611514116755 9.649876428507395E-6 4.8249382142536975E-6 1.978224667844016E-4 4.8249382142536975E-6 38-39 23.841602296361792 9.649876428507395E-6 4.8249382142536975E-6 2.2677209606992378E-4 4.8249382142536975E-6 40-41 25.019121230143085 9.649876428507395E-6 4.8249382142536975E-6 2.605466635696997E-4 4.8249382142536975E-6 42-43 25.944681890376053 9.649876428507395E-6 4.8249382142536975E-6 2.967337001766024E-4 9.649876428507395E-6 44-45 26.659315551634705 9.649876428507395E-6 4.8249382142536975E-6 2.9914616928372926E-4 9.649876428507395E-6 46-47 27.241222400026558 9.649876428507395E-6 4.8249382142536975E-6 2.9914616928372926E-4 9.649876428507395E-6 48-49 27.762045530625954 9.649876428507395E-6 4.8249382142536975E-6 3.2809579856925145E-4 1.2062345535634243E-5 50-51 28.28903010732496 9.649876428507395E-6 4.8249382142536975E-6 3.787576498189152E-4 1.4474814642761092E-5 52-53 28.855436841703522 9.649876428507395E-6 4.8249382142536975E-6 4.221820937471985E-4 1.4474814642761092E-5 54-55 29.45028658685511 9.649876428507395E-6 4.8249382142536975E-6 5.042060433895114E-4 1.4474814642761092E-5 56-57 30.084044633766442 9.649876428507395E-6 4.8249382142536975E-6 5.186808580322725E-4 1.4474814642761092E-5 58-59 30.765494782456564 9.649876428507395E-6 4.8249382142536975E-6 5.379806108892873E-4 1.4474814642761092E-5 60-61 31.474570114892394 9.649876428507395E-6 4.8249382142536975E-6 5.645177710676826E-4 1.4474814642761092E-5 62-63 32.15092030389005 1.2062345535634243E-5 4.8249382142536975E-6 6.007048076745853E-4 1.4474814642761092E-5 64-65 32.793536937362745 1.4474814642761092E-5 4.8249382142536975E-6 6.754913499955177E-4 1.4474814642761092E-5 66-67 33.37747025980458 1.4474814642761092E-5 4.8249382142536975E-6 7.140908557095472E-4 1.4474814642761092E-5 68-69 33.88753619065531 1.929975285701479E-5 4.8249382142536975E-6 7.358030776736888E-4 1.4474814642761092E-5 70-71 34.355157140035246 2.6537160178395335E-5 4.8249382142536975E-6 7.526903614235767E-4 1.4474814642761092E-5 72-73 34.799811383515326 2.8949629285522183E-5 4.8249382142536975E-6 7.623402378520842E-4 1.4474814642761092E-5 74-75 35.21475365747204 2.8949629285522183E-5 4.8249382142536975E-6 7.840524598162258E-4 1.4474814642761092E-5 76-77 35.61712455491151 2.8949629285522183E-5 4.8249382142536975E-6 8.250644346373822E-4 1.4474814642761092E-5 78-79 36.00688306385892 3.377456749977588E-5 4.8249382142536975E-6 8.757262858870461E-4 1.4474814642761092E-5 80-81 36.38755380167979 3.377456749977588E-5 4.8249382142536975E-6 9.818749266006276E-4 1.4474814642761092E-5 82-83 36.7722316506876 3.377456749977588E-5 4.8249382142536975E-6 0.0010228869014217838 1.4474814642761092E-5 84-85 37.165027458240885 3.377456749977588E-5 4.8249382142536975E-6 0.0010590739380286866 1.929975285701479E-5 86-87 37.5568848153115 3.377456749977588E-5 4.8249382142536975E-6 0.001085611098207082 1.929975285701479E-5 88 37.85089725033526 3.377456749977588E-5 4.8249382142536975E-6 0.0010904360364213355 1.929975285701479E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCTGA 7370 0.0 40.813946 9 GCTTGCT 1985 0.0 35.75266 7 TGAAAAA 16845 0.0 34.40825 1 CTTGCTG 5490 0.0 33.30191 8 GCCTGCT 2010 0.0 33.203526 7 TACCCGA 14380 0.0 29.808378 6 CTGCTGA 8775 0.0 29.619253 9 CCTGCTG 7045 0.0 29.15361 8 ACCCGAC 15245 0.0 28.702131 7 CCCGACG 17500 0.0 27.39391 8 GTTGCTG 2570 0.0 26.151392 8 CATTGCC 1245 0.0 25.670887 3 ACTGCTG 1465 0.0 25.665176 8 TCGCGAC 1145 0.0 25.450047 6 CTACCCG 15665 0.0 25.352962 5 GTTTGCC 985 0.0 24.812382 3 ATGCCGC 1215 0.0 24.370861 2 GAAAAAA 134270 0.0 24.328415 1 GCTGAAC 15690 0.0 24.173899 10-11 GTACGAC 1050 0.0 24.17168 6 >>END_MODULE