##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248481_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20725654
Sequences flagged as poor quality	0
Sequence length	100
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30356354496703	34.0	31.0	34.0	30.0	34.0
2	32.51599954336785	34.0	31.0	34.0	31.0	34.0
3	32.66283196660525	34.0	31.0	34.0	31.0	34.0
4	36.17622681532752	37.0	35.0	37.0	35.0	37.0
5	36.09279822967227	37.0	35.0	37.0	35.0	37.0
6	36.07873411377031	37.0	35.0	37.0	35.0	37.0
7	36.07116286897388	37.0	36.0	37.0	35.0	37.0
8	36.06545747603429	37.0	35.0	37.0	35.0	37.0
9	37.80889510169378	39.0	38.0	39.0	35.0	39.0
10-11	37.87419340301638	39.0	38.0	39.0	35.0	39.0
12-13	37.85215540122401	39.0	38.0	39.0	35.0	39.0
14-15	39.288631808675376	41.0	39.0	41.0	36.0	41.0
16-17	39.19340086928017	41.0	39.0	41.0	36.0	41.0
18-19	38.87302803568949	40.0	38.0	41.0	35.0	41.0
20-21	38.437608868699634	40.0	37.0	41.0	34.5	41.0
22-23	38.04747927375416	39.5	36.0	41.0	34.0	41.0
24-25	37.675572770827884	39.0	35.0	41.0	34.0	41.0
26-27	37.459064934693984	39.0	35.0	41.0	33.0	41.0
28-29	37.308464596581615	39.0	35.0	41.0	33.0	41.0
30-31	37.22290280924308	39.0	35.0	41.0	33.0	41.0
32-33	37.236333507256276	39.0	35.0	41.0	33.0	41.0
34-35	37.18188608185778	39.0	35.0	41.0	33.0	41.0
36-37	37.148464651585904	39.0	35.0	41.0	33.0	41.0
38-39	37.03304269674675	39.0	35.0	41.0	33.0	41.0
40-41	36.977298062584666	39.0	35.0	41.0	33.0	41.0
42-43	36.88939388836656	39.0	35.0	41.0	33.0	41.0
44-45	36.70225383478852	38.0	35.0	41.0	33.0	41.0
46-47	36.45518640811045	38.0	35.0	40.0	32.0	41.0
48-49	36.15141133785211	37.0	35.0	40.0	31.5	41.0
50-51	35.81964648739191	36.5	35.0	40.0	31.0	41.0
52-53	35.495086668917665	35.5	35.0	39.0	31.0	41.0
54-55	35.16442011914316	35.0	35.0	39.0	30.5	41.0
56-57	34.83228987611199	35.0	35.0	38.0	30.0	40.5
58-59	34.560463447860315	35.0	34.5	37.0	30.0	40.0
60-61	34.301544935566326	35.0	34.0	36.5	29.5	40.0
62-63	34.06027404491071	35.0	34.0	36.0	29.0	39.5
64-65	33.848526058574556	35.0	34.0	35.5	29.0	39.0
66-67	33.650119436520555	35.0	34.0	35.0	29.0	39.0
68-69	33.48053996269551	35.0	33.0	35.0	29.0	38.0
70-71	33.32535115659077	35.0	33.0	35.0	29.0	37.0
72-73	33.177457488193134	35.0	33.0	35.0	28.0	37.0
74-75	33.03854522515912	35.0	33.0	35.0	27.5	36.0
76-77	32.90284347601287	35.0	33.0	35.0	27.0	36.0
78-79	32.78147201048517	35.0	33.0	35.0	27.0	36.0
80-81	32.66580622739336	35.0	33.0	35.0	27.0	36.0
82-83	32.53565752376258	35.0	33.0	35.0	27.0	35.0
84-85	32.39059705908436	35.0	33.0	35.0	26.5	35.0
86-87	32.28221208363316	35.0	33.0	35.0	25.5	35.0
88-89	32.179310626337774	35.0	33.0	35.0	25.0	35.0
90-91	32.078978738137764	35.0	33.0	35.0	25.0	35.0
92-93	31.913407919479887	35.0	33.0	35.0	25.0	35.0
94-95	31.714368265532176	35.0	32.0	35.0	24.0	35.0
96-97	31.510095773093575	35.0	32.0	35.0	23.5	35.0
98-99	31.14366509254666	34.5	31.5	35.0	21.5	35.0
100	29.445367707093826	33.0	29.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2651827298967717
1101	2	0.2552484944894502
1101	3	0.2617846857144812
1101	4	0.1639479061216278
1101	5	0.18246902777952556
1101	6	0.18798925920674492
1101	7	0.23043775305055902
1101	8	0.221310923599205
1101	9	0.26847954258294493
1101	10-11	0.27624228062469314
1101	12-13	0.2904794809916069
1101	14-15	0.3954614922226014
1101	16-17	0.41313555014814085
1101	18-19	0.45227062611083113
1101	20-21	0.4413836907536819
1101	22-23	0.4349013939730071
1101	24-25	0.44688281346615355
1101	26-27	0.4857188638356007
1101	28-29	0.4858498989910558
1101	30-31	0.4924105737700728
1101	32-33	0.4912521209539946
1101	34-35	0.4687793925971988
1101	36-37	0.49480166972753636
1101	38-39	0.5121471108125775
1101	40-41	0.46625878219231254
1101	42-43	0.45381057338382647
1101	44-45	0.44419824879945224
1101	46-47	0.4516317460047361
1101	48-49	0.45988842518168127
1101	50-51	0.4404926483993279
1101	52-53	0.4429173564218445
1101	54-55	0.46241062912118736
1101	56-57	0.4648739324298887
1101	58-59	0.4501769111373548
1101	60-61	0.4269909673843557
1101	62-63	0.45111587057478175
1101	64-65	0.3814596909902548
1101	66-67	0.4087961170437282
1101	68-69	0.4103349237963485
1101	70-71	0.4244025740762254
1101	72-73	0.42257138202667477
1101	74-75	0.4272328417795208
1101	76-77	0.41286458220138655
1101	78-79	0.4431270705410455
1101	80-81	0.4137958413637861
1101	82-83	0.3986995834034772
1101	84-85	0.3900414864147521
1101	86-87	0.425835586271468
1101	88-89	0.41320505448405953
1101	90-91	0.44004427520059153
1101	92-93	0.44732741577761814
1101	94-95	0.4368241313598169
1101	96-97	0.4218940891028211
1101	98-99	0.5451776749277357
1101	100	0.9663266835829525
1102	1	0.2155821899571393
1102	2	0.24513597128472497
1102	3	0.23549744829601593
1102	4	0.13783663213111907
1102	5	0.1604338283849458
1102	6	0.14519250877384593
1102	7	0.20425060707565734
1102	8	0.1907563248203772
1102	9	0.2378639121088284
1102	10-11	0.2564451135634229
1102	12-13	0.27522910813940626
1102	14-15	0.35723833841964847
1102	16-17	0.3817752652306865
1102	18-19	0.40699442364152105
1102	20-21	0.4301372809027342
1102	22-23	0.37445272010580055
1102	24-25	0.36452461974133854
1102	26-27	0.39361470942682075
1102	28-29	0.41462289489789583
1102	30-31	0.4465980981320996
1102	32-33	0.43046705154612397
1102	34-35	0.42987807716524173
1102	36-37	0.3937811113650156
1102	38-39	0.42638815950844844
1102	40-41	0.4372285534895042
1102	42-43	0.422089381625419
1102	44-45	0.4378040465756925
1102	46-47	0.4198315659354037
1102	48-49	0.4182788307871803
1102	50-51	0.4080637586770379
1102	52-53	0.37232427486801356
1102	54-55	0.37964987578195775
1102	56-57	0.3839547100288314
1102	58-59	0.3594317468106638
1102	60-61	0.35169132034439343
1102	62-63	0.33316870790790176
1102	64-65	0.32284337686991194
1102	66-67	0.339662361893744
1102	68-69	0.3716859402421022
1102	70-71	0.38781200302700114
1102	72-73	0.3723594751769923
1102	74-75	0.3769412455293022
1102	76-77	0.38546059625538476
1102	78-79	0.3811575387548487
1102	80-81	0.3449196074963936
1102	82-83	0.3501069956314211
1102	84-85	0.3363025554120398
1102	86-87	0.36124574046421287
1102	88-89	0.39218339584694206
1102	90-91	0.3757664914219987
1102	92-93	0.3544224761484749
1102	94-95	0.35838483062457627
1102	96-97	0.3572784036149699
1102	98-99	0.551641580218245
1102	100	0.9516526768323637
1103	1	0.21313287283342675
1103	2	0.1939674777711673
1103	3	0.20424084223953543
1103	4	0.12191894947706317
1103	5	0.13501809873310577
1103	6	0.12192456580525146
1103	7	0.1664322461318335
1103	8	0.15806462761772622
1103	9	0.1669128131458848
1103	10-11	0.20704510505547802
1103	12-13	0.23146544568835736
1103	14-15	0.3135260775662658
1103	16-17	0.33197934759320447
1103	18-19	0.3186188111284025
1103	20-21	0.32650116059524237
1103	22-23	0.3035002903632531
1103	24-25	0.3098228034687267
1103	26-27	0.332160367465022
1103	28-29	0.32733348590834765
1103	30-31	0.3789660099425163
1103	32-33	0.3934951523912318
1103	34-35	0.3480270436683739
1103	36-37	0.3553452498599512
1103	38-39	0.40387472026572624
1103	40-41	0.36080228464039976
1103	42-43	0.351783170555116
1103	44-45	0.38491577233143914
1103	46-47	0.370419336968034
1103	48-49	0.3508560847080915
1103	50-51	0.3376443529112265
1103	52-53	0.38648712037974775
1103	54-55	0.3780812605720172
1103	56-57	0.36308370065790996
1103	58-59	0.3567220668289721
1103	60-61	0.357231453106138
1103	62-63	0.3748967851490903
1103	64-65	0.3689825367633688
1103	66-67	0.34465735688245047
1103	68-69	0.3672957930557459
1103	70-71	0.3526395920897869
1103	72-73	0.3434139012395434
1103	74-75	0.3698952493689305
1103	76-77	0.3479889539077021
1103	78-79	0.3748120126526828
1103	80-81	0.35760884203522636
1103	82-83	0.32212509247729315
1103	84-85	0.2884894805682663
1103	86-87	0.34289128364503796
1103	88-89	0.36234398411552604
1103	90-91	0.36829205700960443
1103	92-93	0.35662668261670305
1103	94-95	0.3475421352995802
1103	96-97	0.34607399599259736
1103	98-99	0.5208834662585886
1103	100	0.9267557855862343
1104	1	0.15939852456760661
1104	2	0.16152875722296045
1104	3	0.17779990166026494
1104	4	0.10787200537958341
1104	5	0.11918106389585148
1104	6	0.1295180638026565
1104	7	0.1408926104085495
1104	8	0.15885581443249208
1104	9	0.18773949147613678
1104	10-11	0.1903455374904226
1104	12-13	0.19503961592124597
1104	14-15	0.27063691186388894
1104	16-17	0.26738435529826177
1104	18-19	0.26517320936433464
1104	20-21	0.2742344057852293
1104	22-23	0.2742498264504931
1104	24-25	0.2539288102122228
1104	26-27	0.261797262548356
1104	28-29	0.2947530441748114
1104	30-31	0.30400284766786
1104	32-33	0.31181207639385633
1104	34-35	0.30415471829175544
1104	36-37	0.3021068378501539
1104	38-39	0.32206312226798417
1104	40-41	0.2789380121881919
1104	42-43	0.2939882653763206
1104	44-45	0.29024141189271546
1104	46-47	0.3084782979948315
1104	48-49	0.2722973788451455
1104	50-51	0.28681351962734425
1104	52-53	0.2567845916421376
1104	54-55	0.27344319689841967
1104	56-57	0.28054140892538015
1104	58-59	0.26065378227087166
1104	60-61	0.25076065928742963
1104	62-63	0.26700028063872594
1104	64-65	0.22600222407765358
1104	66-67	0.2435820490128009
1104	68-69	0.22685776415400483
1104	70-71	0.2066712957525354
1104	72-73	0.2036616261723836
1104	74-75	0.24242963473614765
1104	76-77	0.25718079295633345
1104	78-79	0.24451339588141252
1104	80-81	0.2538907852475134
1104	82-83	0.2610710662489595
1104	84-85	0.27068173939225204
1104	86-87	0.25518055945933327
1104	88-89	0.2628838682311141
1104	90-91	0.271579993095159
1104	92-93	0.24864091737595473
1104	94-95	0.23809034338525592
1104	96-97	0.2509411797860537
1104	98-99	0.42054411804060976
1104	100	0.7783175678757743
1105	1	0.15897239501191507
1105	2	0.16539315472851257
1105	3	0.16179653524626048
1105	4	0.12086275351750686
1105	5	0.08095018904418794
1105	6	0.1007561429026893
1105	7	0.15465188161265075
1105	8	0.1433548631737409
1105	9	0.16915759122140628
1105	10-11	0.15915794243600345
1105	12-13	0.1718413035904831
1105	14-15	0.2220177515242483
1105	16-17	0.24467321294407895
1105	18-19	0.2494107492489661
1105	20-21	0.2712450283016281
1105	22-23	0.2605410812314446
1105	24-25	0.2846268743826599
1105	26-27	0.2947401244534973
1105	28-29	0.300737589531046
1105	30-31	0.30069452950711195
1105	32-33	0.2484154682424915
1105	34-35	0.24362978375160793
1105	36-37	0.2710300185125476
1105	38-39	0.3049328765078059
1105	40-41	0.24298592410248432
1105	42-43	0.252467519359314
1105	44-45	0.273370580248816
1105	46-47	0.2783471797044328
1105	48-49	0.2643677499068744
1105	50-51	0.2509755681058152
1105	52-53	0.1902240924867371
1105	54-55	0.1972238790718066
1105	56-57	0.1853626588177235
1105	58-59	0.20023705607931674
1105	60-61	0.18507482470658232
1105	62-63	0.1797911777145913
1105	64-65	0.17155389075205107
1105	66-67	0.20467865091297455
1105	68-69	0.17874509963985474
1105	70-71	0.1596702017936451
1105	72-73	0.17103785169898345
1105	74-75	0.1925682152572037
1105	76-77	0.22917115837392998
1105	78-79	0.20801109193823208
1105	80-81	0.18454459560106073
1105	82-83	0.15921183264772765
1105	84-85	0.1748997375480812
1105	86-87	0.1498475008907576
1105	88-89	0.16575792473501139
1105	90-91	0.2065648403551421
1105	92-93	0.2099079923024938
1105	94-95	0.1866643950485951
1105	96-97	0.2038521558372679
1105	98-99	0.3211680796947327
1105	100	0.7607800837250736
1106	1	0.13847987274694873
1106	2	0.14428127404729452
1106	3	0.12108743038989189
1106	4	0.0765373232305464
1106	5	0.06431040542744881
1106	6	0.05020289064981398
1106	7	0.0851017464295154
1106	8	0.10786417808797921
1106	9	0.10402790767102488
1106	10-11	0.1119772075476746
1106	12-13	0.1212940494172301
1106	14-15	0.13698536028365282
1106	16-17	0.1599194617248827
1106	18-19	0.14263316630574252
1106	20-21	0.1655112577312181
1106	22-23	0.12280160572078813
1106	24-25	0.13763214869688767
1106	26-27	0.1709471773778688
1106	28-29	0.1466437474200788
1106	30-31	0.18831044807883757
1106	32-33	0.16307754015859643
1106	34-35	0.179582777544411
1106	36-37	0.1377886227669194
1106	38-39	0.15140406211627777
1106	40-41	0.106412836179274
1106	42-43	0.12795186482774312
1106	44-45	0.12583393574490742
1106	46-47	0.1153163352347164
1106	48-49	0.09107578350351986
1106	50-51	0.11665491341067025
1106	52-53	0.12417595992906882
1106	54-55	0.10360525381533847
1106	56-57	0.13673271624887917
1106	58-59	0.12486909770345989
1106	60-61	0.11593828391240635
1106	62-63	0.08679158120893504
1106	64-65	0.09884717984427027
1106	66-67	0.10400421768250112
1106	68-69	0.11619991130110918
1106	70-71	0.11656472434226828
1106	72-73	0.10257147281308931
1106	74-75	0.10891849098943851
1106	76-77	0.07962002798898737
1106	78-79	0.10995463812484019
1106	80-81	0.09804047438922936
1106	82-83	0.05229710403133936
1106	84-85	0.03848960400851098
1106	86-87	0.03976459577353353
1106	88-89	0.03863991003349554
1106	90-91	0.04054018385557612
1106	92-93	0.026682507924217447
1106	94-95	0.033619454655433145
1106	96-97	0.04732359274454012
1106	98-99	0.19297257880346308
1106	100	0.5928481881932477
1107	1	0.0770940031729026
1107	2	0.10146932727958813
1107	3	0.091455327403267
1107	4	0.047060794151882135
1107	5	0.020963140878450304
1107	6	0.06040525146235609
1107	7	0.09839326435405837
1107	8	0.09222744885946099
1107	9	0.1154039886740108
1107	10-11	0.12208700633269842
1107	12-13	0.1343562882485685
1107	14-15	0.14595887953998954
1107	16-17	0.10774587269082048
1107	18-19	0.12912892657899278
1107	20-21	0.16408129392529958
1107	22-23	0.1664421677212573
1107	24-25	0.14245153723840787
1107	26-27	0.19552692874947297
1107	28-29	0.16968230926784855
1107	30-31	0.21503710747784766
1107	32-33	0.1739269999124673
1107	34-35	0.18460807275952362
1107	36-37	0.17130052130962525
1107	38-39	0.1934755914906816
1107	40-41	0.17596493478674802
1107	42-43	0.18065331754724667
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2216	46-47	-0.5225392251734036
2216	48-49	-0.4768739956675958
2216	50-51	-0.467117857249022
2216	52-53	-0.41418222945319627
2216	54-55	-0.4014067209621004
2216	56-57	-0.3795935638700527
2216	58-59	-0.3429994289519769
2216	60-61	-0.3506192786789484
2216	62-63	-0.3564260823292571
2216	64-65	-0.3294094067409077
2216	66-67	-0.3309007838713427
2216	68-69	-0.36846063721917943
2216	70-71	-0.37066741121392255
2216	72-73	-0.3817573563568075
2216	74-75	-0.3962255314567571
2216	76-77	-0.38005900710564333
2216	78-79	-0.3946969350276106
2216	80-81	-0.4014955126533053
2216	82-83	-0.39428449528084997
2216	84-85	-0.44756385341920435
2216	86-87	-0.4685523590754741
2216	88-89	-0.4756824177185379
2216	90-91	-0.4909351305624874
2216	92-93	-0.4883693103412874
2216	94-95	-0.46282638056665704
2216	96-97	-0.506888598466567
2216	98-99	-0.36196862187478374
2216	100	0.18081336606352139
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	8.0
7	50.0
8	177.0
9	434.0
10	959.0
11	1863.0
12	2975.0
13	4520.0
14	6717.0
15	10848.0
16	17348.0
17	27192.0
18	39066.0
19	52289.0
20	66202.0
21	82830.0
22	102972.0
23	124378.0
24	146757.0
25	171944.0
26	199930.0
27	234192.0
28	280055.0
29	346721.0
30	404931.0
31	502163.0
32	647835.0
33	918583.0
34	1320251.0
35	4997276.0
36	3141564.0
37	4747943.0
38	1813326.0
39	310756.0
40	597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.957317575173448	22.57506776171542	32.762807502292986	24.704807160818145
2	16.87042950128913	22.821608150886934	39.900917461681615	20.407044886142316
3	13.656529246314737	18.74612979643489	44.44058556608154	23.156755391168836
4	13.730577198098587	16.738749683966333	47.50708301408304	22.02359010385204
5	12.46470191873284	17.945002953975898	49.1477876072023	20.442507520088967
6	11.826120110678156	13.246831725332273	45.5217044196861	29.405343744303465
7	15.799882599603372	14.458863397024768	42.88503513568257	26.85621886768929
8	12.665554486242026	13.073522312010033	43.060469889152834	31.200453312595105
9	12.650592352839626	13.82935370821109	46.971318733777956	26.54873520517133
10-11	15.55975024961818	21.098702120569996	46.992828308337096	16.34871932147473
12-13	13.91466381233558	27.328605397138777	42.10163121566934	16.6550995748563
14-15	16.285143690251797	24.227391938318977	41.79991557729231	17.687548794136916
16-17	21.265469385561232	25.57351207285213	42.8229392139553	10.338079327631334
18-19	21.478992724620063	19.98045369185714	41.89760631426185	16.64294726926095
20-21	16.414605201839557	19.42805235870044	46.554167109859925	17.60317532960008
22-23	16.478270343275877	20.57366305331594	48.115726360116156	14.832340243292021
24-25	16.53546999980434	21.55342736942599	46.15217092411787	15.758931706651802
26-27	20.491770808489363	20.97516340386476	43.170388411045636	15.362677376600246
28-29	17.969052246770254	23.98536279431492	46.05530163241543	11.990283326499394
30-31	18.286857837673523	22.44013722623511	45.58164574979855	13.691359186292818
32-33	19.025329624615615	23.814702659313117	44.524275021745616	12.635692694325652
34-35	19.0547600277243	23.951962914732096	44.29171851985205	12.70155853769156
36-37	19.292832956718502	22.73732427965176	44.118929257143655	13.850913506486087
38-39	19.706419960848727	22.58217524120697	43.55058233070374	14.160822467240566
40-41	19.720766482038133	23.26322418910832	43.54886061834415	13.467148710509399
42-43	19.565827305368565	23.466112413576386	43.79507432898242	13.17298595207263
44-45	20.03137138794893	22.073925419224878	43.72283454019922	14.171868652626971
46-47	20.373496738960885	21.342783508574804	44.208507208571504	14.075212543892803
48-49	19.281912898093207	21.283290489672286	45.02203302118379	14.41276359105072
50-51	19.03518121532528	21.130898915196443	45.412245569952404	14.421674299525874
52-53	18.581594298700608	21.377920975049577	45.43758293084231	14.602901795407499
54-55	18.247320171573094	21.755661195150257	45.21453970911241	14.782478924164241
56-57	17.896420461618785	21.779416689264764	45.18264408336815	15.1415187657483
58-59	17.537111671381357	21.84553115320094	45.5961492136293	15.021207961788397
60-61	16.868157153628292	21.645644903107538	45.83873652892707	15.6474614143371
62-63	16.71188444015421	21.48649865468619	45.892592237646916	15.90902466751268
64-65	16.634843480421605	21.397097510201103	45.78614794716261	16.18191106221468
66-67	16.213902511389485	21.11895335315382	46.291635070590914	16.375509064865778
68-69	16.15866988714105	20.552366365875134	46.86860257034775	16.42036117663607
70-71	15.916667591526101	20.40705235074337	47.29730499178001	16.37897506595052
72-73	15.61456171287248	19.904253350565767	48.189682397415424	16.29150253914633
74-75	15.466615513962005	19.537520940718892	48.64701586718973	16.348847678129374
76-77	15.606814118762363	19.438824705112857	48.69377448788346	16.260586688241318
78-79	15.816494041852664	19.624797983512096	48.749150483633215	15.809557491002026
80-81	15.958476306511976	19.498294575513665	49.03964848049845	15.503580637475912
82-83	16.226694660333983	19.58219502196456	48.948535301463245	15.242575016238211
84-85	16.264414529190624	19.898582012207825	48.44619639358799	15.39080706501356
86-87	16.31564316558621	20.302147098131254	48.13979957934022	15.242410156942318
88-89	16.334561126420258	20.80996367770072	47.86846736766666	14.98700782821236
90-91	16.388602974979563	21.323105910722916	47.591759667197415	14.696531447100107
92-93	16.233245889901767	21.569430453809044	47.30791463785866	14.889409018430532
94-95	16.26301523076179	21.984931481897647	46.98973546317677	14.76231782416379
96-97	15.904487547842574	22.625784935217435	47.15488411989666	14.314843397043333
98-99	15.720147246281279	23.23115678966271	47.02700403739804	14.021691926657976
100	15.537400630655787	23.94732316055536	46.535435074013115	13.979841134775736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	676401.0
1	1392170.5
2	1765078.5
3	908208.5
4	240560.5
5	64642.0
6	35327.0
7	26371.5
8	23963.5
9	25038.5
10	29211.5
11	36656.5
12	49026.5
13	66005.5
14	87583.0
15	113842.5
16	144249.0
17	177190.0
18	209275.0
19	236850.0
20	258645.0
21	275566.0
22	289161.5
23	300419.5
24	308548.0
25	312223.5
26	310881.5
27	303757.5
28	291896.0
29	275406.5
30	255526.5
31	236180.0
32	216574.5
33	196808.5
34	177874.0
35	160351.0
36	144446.5
37	129060.0
38	114810.0
39	102557.0
40	92855.0
41	85752.5
42	82145.5
43	86679.5
44	104930.0
45	138956.5
46	187390.5
47	248220.0
48	323222.5
49	424126.5
50	558876.0
51	725859.0
52	902986.0
53	1046567.5
54	1110525.0
55	1067218.0
56	925098.0
57	722729.0
58	512155.0
59	331931.0
60	197374.0
61	107937.5
62	54716.5
63	26153.0
64	12336.5
65	6099.0
66	3406.5
67	2299.5
68	1751.0
69	1337.5
70	987.5
71	746.5
72	566.5
73	445.5
74	345.5
75	255.5
76	173.5
77	100.5
78	62.0
79	40.0
80	33.0
81	23.5
82	14.0
83	14.0
84	17.5
85	13.0
86	4.5
87	5.5
88	7.5
89	3.5
90	2.5
91	4.5
92	3.0
93	1.5
94	1.0
95	0.0
96	0.0
97	1.0
98	1.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01821896669702196
2	0.11250790927996772
3	0.0
4	6.754913499955177E-5
5	0.03770206720617839
6	0.0016211792399892424
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.215631989224561E-4
14-15	9.046759151725682E-4
16-17	2.4124691071268487E-6
18-19	4.8249382142536975E-6
20-21	5.814050548175705E-4
22-23	5.210933271393993E-4
24-25	0.002882900583016584
26-27	0.0022725458989134913
28-29	0.0031844592214074407
30-31	0.003638003413547288
32-33	0.003816526127474675
34-35	0.004219408468364858
36-37	0.003271308109264007
38-39	0.0019444501003442398
40-41	0.003408818848370237
42-43	0.005145796605501568
44-45	0.0050034609281810844
46-47	0.012455578000095922
48-49	0.00520610833317974
50-51	0.006952735966739577
52-53	0.0026730157706965486
54-55	0.0012544839357059613
56-57	0.005760976227818915
58-59	0.0025885793519471087
60-61	0.014298704397940832
62-63	0.012583438862773643
64-65	0.00980668692047064
66-67	0.01198755899331331
68-69	0.009645051490293141
70-71	0.008607689774228596
72-73	0.005640352772462572
74-75	0.0037658642762250106
76-77	4.7284394499686236E-4
78-79	0.010122720373504257
80-81	0.006472654614421335
82-83	0.010409804197252353
84-85	0.008759675327977588
86-87	0.0074448796645934555
88-89	0.009751200131006723
90-91	0.005671714870855221
92-93	0.00950754075118691
94-95	0.0031675719376575524
96-97	0.005659652525319587
98-99	0.4513922696962904
100	1.756238910482632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.0725654E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.35726025643034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.16945927213347	64.8479063370395
2	6.730041217313352	9.470145229260375
3	0.8020143379648936	1.6928259451675407
4	0.14107571762321794	0.3970280392271768
5	0.04307325800890933	0.15152582119126026
6	0.023186364329897343	0.09787974417354
7	0.01102104359124064	0.05427873025724669
8	0.0123757857916299	0.06965811054556302
9	0.005707472279656399	0.036140590132753225
>10	0.047309653900203634	0.6345836088150024
>50	0.00594976013383439	0.29486883619868975
>100	0.006611896459202101	0.9902344658940172
>500	6.574936713336624E-4	0.3183171026763571
>1k	7.207081609097411E-4	1.2233043273874409
>5k	2.1959134852559702E-4	1.1027375619362123
>10k+	5.764272898796916E-4	18.618565550097387
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	159546	0.7697995923313203	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	126415	0.6099445643548812	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123486	0.595812320325332	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122606	0.5915663746967889	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111439	0.5376862896582177	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	109981	0.5306515297418358	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107371	0.5180584410026338	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105741	0.5101937917134003	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99764	0.4813551360068059	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98912	0.47724428864826174	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92370	0.44567954285061406	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90853	0.4383601115795912	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88864	0.42876330947144053	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87750	0.423388328300762	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86096	0.4154078804943864	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81628	0.39385005655310085	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79404	0.3831193939646006	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77670	0.3747529511010847	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76651	0.36983633906076013	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76593	0.36955649264433343	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74609	0.3599838152272541	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71820	0.34652706254770055	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69986	0.33767812586275925	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66026	0.3185713705343146	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65848	0.31771253153217743	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63985	0.3087236716390228	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62458	0.3013559909858574	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61487	0.2966709759798171	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61451	0.296497278204104	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59266	0.28595478820595965	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56971	0.2748815550042474	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50844	0.24531915856551498	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49278	0.2377633053219937	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46787	0.22574438423028773	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43587	0.2103045819446759	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40460	0.1952170001487046	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37178	0.17938155292952396	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33648	0.16234952103320843	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31568	0.15231364954756071	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30355	0.14646099949367097	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28105	0.13560488851160016	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27850	0.13437452926696547	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27691	0.13360736409089913	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27133	0.13091504856734557	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27110	0.13080407498841776	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26842	0.12951099154699774	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26282	0.12680902614701567	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25945	0.12518302196881217	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23505	0.11341017272603315	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22512	0.10861900907927924	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22287	0.10753339798107217	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21517	0.10381819555609681	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21131	0.10195576940539489	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0037248523014038544	0.0	0.0	1.4474814642761092E-5	0.0
2	0.009384504826723442	0.0	0.0	1.929975285701479E-5	0.0
3	0.015135831178113849	0.0	0.0	2.4124691071268487E-5	0.0
4	0.024361113043766917	0.0	0.0	2.8949629285522183E-5	0.0
5	0.04414818466042133	0.0	0.0	2.8949629285522183E-5	0.0
6	0.08679098859799551	0.0	0.0	3.377456749977588E-5	0.0
7	0.14401958075725863	0.0	0.0	3.377456749977588E-5	0.0
8	0.4017291806569771	0.0	0.0	3.377456749977588E-5	0.0
9	1.31238801921522	0.0	0.0	3.377456749977588E-5	0.0
10-11	2.9386117321074643	0.0	0.0	3.377456749977588E-5	0.0
12-13	4.388266831049095	0.0	0.0	4.101197482115643E-5	0.0
14-15	5.6802718987782	0.0	0.0	4.824938214253697E-5	0.0
16-17	6.6130820286780825	0.0	0.0	5.7899258571044366E-5	0.0
18-19	7.4472173471582614	0.0	0.0	7.237407321380546E-5	0.0
20-21	8.487736985284034	0.0	0.0	7.237407321380546E-5	0.0
22-23	9.80896187883866	0.0	0.0	7.237407321380546E-5	0.0
24-25	11.564385374763084	0.0	0.0	7.237407321380546E-5	0.0
26-27	13.567769682925325	0.0	7.237407321380547E-6	7.9611480535186E-5	0.0
28-29	15.855781921284606	0.0	9.649876428507395E-6	8.202394964231285E-5	0.0
30-31	18.32724796042624	0.0	9.649876428507395E-6	8.443641874943971E-5	0.0
32-33	21.24139966825655	0.0	9.649876428507395E-6	8.92613569636934E-5	0.0
34-35	24.19897099507692	0.0	9.649876428507395E-6	9.167382607082026E-5	0.0
36-37	26.225478819630975	0.0	9.649876428507395E-6	9.649876428507395E-5	0.0
38-39	27.610909166002674	0.0	9.649876428507395E-6	1.0132370249932765E-4	0.0
40-41	29.084672068731823	0.0	9.649876428507395E-6	1.1821098624921559E-4	0.0
42-43	30.181503560756152	0.0	9.649876428507395E-6	1.3509826999910353E-4	0.0
44-45	30.96700832697487	0.0	9.649876428507395E-6	1.5439802285611832E-4	0.0
46-47	31.577461922311354	0.0	9.649876428507395E-6	1.6404789928462573E-4	0.0
48-49	32.091264767809015	0.0	9.649876428507395E-6	1.736977757131331E-4	0.0
50-51	32.59367352171372	2.4124691071268487E-6	9.649876428507395E-6	2.07472343212909E-4	0.0
52-53	33.1247158714509	9.649876428507395E-6	9.649876428507395E-6	2.605466635696997E-4	0.0
54-55	33.61870028323352	9.649876428507395E-6	9.649876428507395E-6	3.1120851481936346E-4	0.0
56-57	34.11160149638704	9.649876428507395E-6	9.649876428507395E-6	3.3050826767637827E-4	0.0
58-59	34.596712846793636	9.649876428507395E-6	9.649876428507395E-6	3.401581441048857E-4	0.0
60-61	35.08274093546095	9.649876428507395E-6	9.649876428507395E-6	3.4498308231913934E-4	0.0
62-63	35.568604976228976	9.649876428507395E-6	9.649876428507395E-6	3.6669530428328097E-4	0.0
64-65	36.05347266725576	9.649876428507395E-6	9.649876428507395E-6	3.884075262474226E-4	0.0
66-67	36.50409294683777	9.649876428507395E-6	9.649876428507395E-6	4.366569083899596E-4	0.0
68-69	36.909713440164545	9.649876428507395E-6	9.649876428507395E-6	4.5113172303272073E-4	0.0
70-71	37.29436233954306	9.649876428507395E-6	9.649876428507395E-6	4.607815994612281E-4	0.0
72-73	37.673595245776085	9.649876428507395E-6	9.649876428507395E-6	4.7284394499686236E-4	0.0
74-75	38.0348214825935	9.649876428507395E-6	9.649876428507395E-6	4.897312287467503E-4	0.0
76-77	38.389811486769005	9.649876428507395E-6	9.649876428507395E-6	5.25918265353653E-4	0.0
78-79	38.72718564152427	9.649876428507395E-6	9.649876428507395E-6	6.320669060672344E-4	0.0
80-81	39.062586396549904	9.649876428507395E-6	9.649876428507395E-6	7.044409792810399E-4	0.0
82-83	39.40886497477956	9.649876428507395E-6	9.649876428507395E-6	7.478654232093232E-4	0.0
84-85	39.76295995291632	9.649876428507395E-6	9.649876428507395E-6	7.575152996378304E-4	0.0
86-87	40.119807558304316	1.4474814642761092E-5	9.649876428507395E-6	7.671651760663379E-4	0.0
88	40.385673716255226	1.929975285701479E-5	9.649876428507395E-6	7.671651760663379E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	3630	0.0	59.886906	1
TGCCCTA	15400	0.0	54.20373	6
CTCCCTA	37510	0.0	50.31823	6
TGCTCTA	14315	0.0	50.173985	6
CGCCCTA	15805	0.0	49.664314	6
CCCCTAC	102520	0.0	49.21923	7
GTTACAC	2955	0.0	48.484245	7
CCCCCTA	55040	0.0	48.476627	6
GACTTTT	3670	0.0	48.0797	1
CCGCCTA	6960	0.0	46.974533	6
TCCCCTA	31940	0.0	46.092102	6
CATTTTT	25765	0.0	44.30545	1
TTGCCCT	6895	0.0	43.823265	5
ATGCCCT	5220	0.0	43.661564	5
CCGCCCT	9620	0.0	43.377644	5
CGCCTAC	9585	0.0	42.58787	7
CACCCTA	18280	0.0	42.066364	6
CCTTTTC	7570	0.0	41.770523	1
ACTTTTT	31530	0.0	41.50243	1
TCCCCCT	23035	0.0	41.33132	5
>>END_MODULE