##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248480_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17572229
Sequences flagged as poor quality	0
Sequence length	100
%GC	28
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.484663556342227	31.0	30.0	33.0	23.0	34.0
2	30.02847026407407	31.0	30.0	34.0	25.0	34.0
3	30.475979057636913	31.0	30.0	34.0	26.0	34.0
4	33.93885488289505	35.0	35.0	37.0	29.0	37.0
5	33.752319640268745	35.0	35.0	37.0	28.0	37.0
6	33.480906320990925	35.0	33.0	37.0	27.0	37.0
7	33.632918510224286	35.0	35.0	37.0	28.0	37.0
8	33.64611888451943	35.0	35.0	37.0	28.0	37.0
9	34.886614441457596	37.0	34.0	39.0	27.0	39.0
10-11	35.544397640162785	38.0	35.0	39.0	30.0	39.0
12-13	35.859638068682116	39.0	35.0	39.0	31.0	39.0
14-15	36.77233491550787	39.0	36.0	41.0	30.5	41.0
16-17	36.469917618305566	39.0	36.0	40.5	30.0	41.0
18-19	35.8279056402008	38.0	34.5	40.0	30.0	41.0
20-21	35.28921942685814	37.0	34.0	39.5	28.0	41.0
22-23	34.88509983565545	37.0	34.0	39.0	27.5	40.5
24-25	34.58303175425269	36.0	33.5	39.0	27.0	40.5
26-27	34.33127427943262	35.0	33.0	39.0	25.0	40.0
28-29	34.191482253048264	35.0	33.0	39.0	25.0	40.0
30-31	34.04854554877471	35.0	33.0	39.0	25.0	40.0
32-33	33.909316712182616	35.0	33.0	39.0	25.0	40.0
34-35	33.75621422302202	35.0	33.0	39.0	25.0	40.0
36-37	33.46465934970458	35.0	32.0	38.0	25.0	40.0
38-39	33.22915422397466	35.0	31.0	38.0	24.0	40.0
40-41	32.97129405154008	35.0	31.0	38.0	24.0	40.0
42-43	32.75697573711337	35.0	30.0	38.0	23.0	40.0
44-45	32.4418052484975	35.0	30.0	38.0	23.0	40.0
46-47	32.3740804880246	35.0	30.0	37.5	22.5	40.0
48-49	32.4331315053998	35.0	30.0	37.5	23.0	40.0
50-51	32.321641067846315	35.0	30.0	37.0	23.0	39.0
52-53	32.23877613932757	35.0	30.0	37.0	22.5	39.0
54-55	32.063804995939904	35.0	30.0	37.0	22.0	39.0
56-57	32.3591395263515	35.0	30.0	37.0	23.5	39.0
58-59	32.51000675554592	35.0	31.0	36.5	24.5	39.0
60-61	32.357944971010795	35.0	30.0	36.0	25.0	39.0
62-63	32.14108065061069	34.5	30.0	35.5	24.5	39.0
64-65	31.940272375234812	34.0	30.0	35.0	24.0	39.0
66-67	31.743935331140975	34.0	30.0	35.0	23.5	38.0
68-69	31.58971590912001	34.0	30.0	35.0	23.0	37.5
70-71	31.454616656771318	34.0	30.0	35.0	24.0	37.0
72-73	31.346601503998155	34.0	30.0	35.0	23.5	36.0
74-75	31.243138135748175	34.0	30.0	35.0	23.0	36.0
76-77	31.105175302461628	34.0	30.0	35.0	23.0	36.0
78-79	30.981167500150377	34.0	30.0	35.0	23.0	35.0
80-81	30.904231699916956	34.0	30.0	35.0	23.0	35.0
82-83	30.821531690714934	34.0	30.0	35.0	21.5	35.0
84-85	30.74971752303023	34.0	30.0	35.0	20.5	35.0
86-87	30.641961472275373	34.0	29.5	35.0	20.0	35.0
88-89	30.53018723464166	34.0	29.0	35.0	20.0	35.0
90-91	30.35952766720716	34.0	29.0	35.0	19.5	35.0
92-93	30.16917483832017	34.0	29.0	35.0	18.5	35.0
94-95	29.985547507945633	34.0	29.0	35.0	17.5	35.0
96-97	29.744402289544485	34.0	28.5	35.0	15.0	35.0
98-99	29.445468187331272	34.0	28.0	35.0	12.5	35.0
100	27.824597437240318	32.0	25.0	35.0	8.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31799141575266177
1101	2	0.2819193599955696
1101	3	0.3507472043912756
1101	4	0.2068193747415421
1101	5	0.15663177366266723
1101	6	0.15191562952036008
1101	7	0.19874742175804982
1101	8	0.2500331786451113
1101	9	0.32951606921034937
1101	10-11	0.4337175636601316
1101	12-13	0.5009178559343042
1101	14-15	0.6485109396235487
1101	16-17	0.6875188013479061
1101	18-19	0.6880625397149629
1101	20-21	0.5961290068404352
1101	22-23	0.5482587489042388
1101	24-25	0.5970698689013076
1101	26-27	0.6010385622162744
1101	28-29	0.6129843173279781
1101	30-31	0.6188351547845912
1101	32-33	0.6156108368810536
1101	34-35	0.6301998895808865
1101	36-37	0.6166929773409962
1101	38-39	0.5615867593144444
1101	40-41	0.5723851580496131
1101	42-43	0.6298818787184928
1101	44-45	0.5044582102875239
1101	46-47	0.557503537994755
1101	48-49	0.6044251525000774
1101	50-51	0.5423085260949136
1101	52-53	0.5436328150081806
1101	54-55	0.5273892726017664
1101	56-57	0.47425574095237266
1101	58-59	0.4473110754112426
1101	60-61	0.45816271208900616
1101	62-63	0.45008974758943765
1101	64-65	0.44461984306025215
1101	66-67	0.47106968643570823
1101	68-69	0.4597829330494392
1101	70-71	0.45448972653539244
1101	72-73	0.44829771742347546
1101	74-75	0.38701666002137003
1101	76-77	0.39480269301987647
1101	78-79	0.39625743821203585
1101	80-81	0.3591279773708109
1101	82-83	0.4216395575150571
1101	84-85	0.38901824875488344
1101	86-87	0.35353060022976734
1101	88-89	0.35599568779998947
1101	90-91	0.3702514371808583
1101	92-93	0.3397006044773505
1101	94-95	0.3059666191015893
1101	96-97	0.2653066162929463
1101	98-99	0.2490654274109687
1101	100	0.2883469182598972
1102	1	0.270302682723635
1102	2	0.22883270841704118
1102	3	0.2590338605791551
1102	4	0.17289614570072587
1102	5	0.18017570375560155
1102	6	0.09749513623317796
1102	7	0.20909242370429837
1102	8	0.2266284456663712
1102	9	0.2137162202563374
1102	10-11	0.28493705927009927
1102	12-13	0.3606684136206866
1102	14-15	0.3901579660469423
1102	16-17	0.4114460781607363
1102	18-19	0.443851485986265
1102	20-21	0.3691188936958696
1102	22-23	0.33012504735422965
1102	24-25	0.34249334707895684
1102	26-27	0.31850020883751284
1102	28-29	0.3187990705309929
1102	30-31	0.3055917278964273
1102	32-33	0.2944069466500139
1102	34-35	0.35789121181466754
1102	36-37	0.2819969162320959
1102	38-39	0.26812833807797176
1102	40-41	0.275949384245358
1102	42-43	0.30443227073020296
1102	44-45	0.2208690786395664
1102	46-47	0.2837042211362544
1102	48-49	0.29217020283701345
1102	50-51	0.31630217080476797
1102	52-53	0.34446120058655083
1102	54-55	0.3193285783574211
1102	56-57	0.31944021949951207
1102	58-59	0.3674106186516468
1102	60-61	0.359705065125965
1102	62-63	0.3516017189528071
1102	64-65	0.3773000471092658
1102	66-67	0.3780245352767331
1102	68-69	0.4111452455345983
1102	70-71	0.35374286976000135
1102	72-73	0.36688395457210987
1102	74-75	0.3068857161493277
1102	76-77	0.32262167870560177
1102	78-79	0.33731797169035005
1102	80-81	0.3301694619270208
1102	82-83	0.36601746071303154
1102	84-85	0.3205748462528746
1102	86-87	0.3132773828889128
1102	88-89	0.32974139852350604
1102	90-91	0.32781450782168164
1102	92-93	0.3319131395207542
1102	94-95	0.2796978273976407
1102	96-97	0.2641990302241979
1102	98-99	0.24273218226137416
1102	100	0.2076891551202955
1103	1	0.2445224004318014
1103	2	0.2183392373945523
1103	3	0.20144162162723234
1103	4	0.16634004300641436
1103	5	0.17239353169152594
1103	6	0.11192855377036182
1103	7	0.15743088481274725
1103	8	0.23157719249018527
1103	9	0.13918107524497714
1103	10-11	0.18573011574153497
1103	12-13	0.2719770795859944
1103	14-15	0.3014771633969673
1103	16-17	0.31430151243833393
1103	18-19	0.3231053236492656
1103	20-21	0.25844574895950956
1103	22-23	0.17555227761106806
1103	24-25	0.18412044234307245
1103	26-27	0.20192558290500529
1103	28-29	0.23655683482978418
1103	30-31	0.20100567891847732
1103	32-33	0.18681802138490866
1103	34-35	0.2685109300195805
1103	36-37	0.20458884235588926
1103	38-39	0.17617555611239055
1103	40-41	0.17396910786202113
1103	42-43	0.19647823647260054
1103	44-45	0.1554590234077864
1103	46-47	0.1531501492623235
1103	48-49	0.20539702558296824
1103	50-51	0.21006815776946297
1103	52-53	0.19228673813275066
1103	54-55	0.19162487709080978
1103	56-57	0.23449419201531185
1103	58-59	0.2247048216902101
1103	60-61	0.2660822173629285
1103	62-63	0.30867604151112005
1103	64-65	0.2360559830820108
1103	66-67	0.26666768048658795
1103	68-69	0.2815169446447001
1103	70-71	0.291240533169141
1103	72-73	0.25274825461047357
1103	74-75	0.25346055337419315
1103	76-77	0.26072606076737515
1103	78-79	0.2616409786520322
1103	80-81	0.28815971060990364
1103	82-83	0.3024579731956436
1103	84-85	0.24943959201666033
1103	86-87	0.32513687123363155
1103	88-89	0.3130158306145212
1103	90-91	0.25751854775075955
1103	92-93	0.2248230058925067
1103	94-95	0.21999565674125066
1103	96-97	0.15438598093075484
1103	98-99	0.13177349986930764
1103	100	0.07596554944912981
1104	1	0.09795821726697795
1104	2	0.12435042620438352
1104	3	0.16932368491934469
1104	4	0.14763582625654692
1104	5	0.09015492119488755
1104	6	0.10641567696251997
1104	7	0.11858754572156016
1104	8	0.12847979930942444
1104	9	0.07183241676810326
1104	10-11	0.1403805086190104
1104	12-13	0.16574175364188903
1104	14-15	0.1878393895522379
1104	16-17	0.16054983848091098
1104	18-19	0.18372669131143482
1104	20-21	0.15488969637634398
1104	22-23	0.18456412394783683
1104	24-25	0.2287875631252163
1104	26-27	0.22962704298109315
1104	28-29	0.14413554309930277
1104	30-31	0.1416752748687813
1104	32-33	0.1768392842998452
1104	34-35	0.17761347755267565
1104	36-37	0.18552863820173826
1104	38-39	0.10705860536290857
1104	40-41	0.09024171870617437
1104	42-43	0.11848036600480327
1104	44-45	0.09818678659296154
1104	46-47	0.14956191406206898
1104	48-49	0.186087747684347
1104	50-51	0.15590480874129042
1104	52-53	0.2226138239641955
1104	54-55	0.20558477788785723
1104	56-57	0.19743102036292726
1104	58-59	0.20134309687357899
1104	60-61	0.22550887740190007
1104	62-63	0.23446693129589846
1104	64-65	0.217886852450917
1104	66-67	0.22872765429274367
1104	68-69	0.2549264679343537
1104	70-71	0.23479996431513683
1104	72-73	0.2522508504033709
1104	74-75	0.23478320130761787
1104	76-77	0.1985863734525708
1104	78-79	0.26766083313707156
1104	80-81	0.24967682852446416
1104	82-83	0.241623414463092
1104	84-85	0.23395336196187344
1104	86-87	0.24770587209366823
1104	88-89	0.2486114180416017
1104	90-91	0.2505561400424554
1104	92-93	0.23115146604215653
1104	94-95	0.20792650637750043
1104	96-97	0.16277002912729444
1104	98-99	0.14325254391491526
1104	100	0.1724775299482708
1105	1	0.12837711801035212
1105	2	0.1103963460216768
1105	3	0.12245978878859987
1105	4	0.09176722959135475
1105	5	0.07638749926027799
1105	6	0.06261455488818513
1105	7	0.07613730027326682
1105	8	0.0812254949324398
1105	9	0.0011875764735975736
1105	10-11	0.0952684799500716
1105	12-13	0.13953338117052994
1105	14-15	0.20894193945078143
1105	16-17	0.20055078116632785
1105	18-19	0.20312175181734915
1105	20-21	0.14491626356952736
1105	22-23	0.12791471471749816
1105	24-25	0.14690388471549198
1105	26-27	0.11943368318033265
1105	28-29	0.1146884984283858
1105	30-31	0.15388318614880347
1105	32-33	0.08096591972759626
1105	34-35	0.12985729231579768
1105	36-37	0.1857731656908257
1105	38-39	0.16688274353973043
1105	40-41	0.148302309535012
1105	42-43	0.15428421268003945
1105	44-45	0.13116219213176095
1105	46-47	0.11073829683331127
1105	48-49	0.12442562350371134
1105	50-51	0.18429352062504734
1105	52-53	0.22253492071703818
1105	54-55	0.18779439000122267
1105	56-57	0.20479056690798814
1105	58-59	0.16422198839467228
1105	60-61	0.19627727125541128
1105	62-63	0.18447785579897413
1105	64-65	0.2163425515687436
1105	66-67	0.188432806555074
1105	68-69	0.20943819140840958
1105	70-71	0.24867024700921192
1105	72-73	0.23123010369412356
1105	74-75	0.2272329372238211
1105	76-77	0.18817432832324243
1105	78-79	0.22352275695055468
1105	80-81	0.20663103377070868
1105	82-83	0.259794662465751
1105	84-85	0.22308564182225865
1105	86-87	0.1809642714560198
1105	88-89	0.22089784049534344
1105	90-91	0.21053015747195403
1105	92-93	0.16528360336394599
1105	94-95	0.16265948396962315
1105	96-97	0.1263466245789644
1105	98-99	0.13733479723619268
1105	100	0.17426141223727498
1106	1	0.04284125982412945
1106	2	0.014350461310087326
1106	3	0.09823371997130081
1106	4	0.04980552485980638
1106	5	0.07132556774529775
1106	6	0.03141677285069733
1106	7	0.11161334751172092
1106	8	0.07486797710073034
1106	9	-0.00534381755382185
1106	10-11	0.1194153001717595
1106	12-13	0.14361252709786498
1106	14-15	0.12218611344306396
1106	16-17	0.0814263094991503
1106	18-19	0.01865709332484755
1106	20-21	0.07380927924728553
1106	22-23	0.04897901767557755
1106	24-25	0.07908787765055791
1106	26-27	0.07535052381523144
1106	28-29	0.05894344360961412
1106	30-31	0.05015961864208407
1106	32-33	0.05497386623426337
1106	34-35	0.16016058606530947
1106	36-37	0.1436163426843038
1106	38-39	0.11476880471575868
1106	40-41	0.1530227332504026
1106	42-43	0.14565076905212493
1106	44-45	0.15278900667756545
1106	46-47	0.1500578209391321
1106	48-49	0.14478801249628503
1106	50-51	0.18966125510351617
1106	52-53	0.19828039892109928
1106	54-55	0.14797387563496045
1106	56-57	0.1551507735928226
1106	58-59	0.1991417549845238
1106	60-61	0.18502857998018385
1106	62-63	0.22526841171888634
1106	64-65	0.2328405689999613
1106	66-67	0.1610476584258933
1106	68-69	0.15267439131556415
1106	70-71	0.1529072416246784
1106	72-73	0.1584257539085172
1106	74-75	0.13323616757324785
1106	76-77	0.15175141346701082
1106	78-79	0.146626366265469
1106	80-81	0.16245869119760314
1106	82-83	0.1758126450091666
1106	84-85	0.15197863095310282
1106	86-87	0.16203464500988574
1106	88-89	0.19497062896855866
1106	90-91	0.19857472372744311
1106	92-93	0.1653213674818126
1106	94-95	0.1824682363858834
1106	96-97	0.19178626902512264
1106	98-99	0.15192576956942716
1106	100	0.13151409340696674
1107	1	-0.015031010977015313
1107	2	-0.002847385081302889
1107	3	0.05695005573558376
1107	4	0.010427059999983612
1107	5	0.050599336622362046
1107	6	-0.026529766900992513
1107	7	0.09188755419985029
1107	8	0.05652132538597243
1107	9	0.018411510658033592
1107	10-11	0.06280000440486333
1107	12-13	0.15289862316360114
1107	14-15	0.198107433567273
1107	16-17	0.17375308071932238
1107	18-19	0.09945724468884976
1107	20-21	0.111117103086535
1107	22-23	0.12598906564971912
1107	24-25	0.14600720225757158
1107	26-27	0.1388912758355758
1107	28-29	0.14649368223178527
1107	30-31	0.145778050943548
1107	32-33	0.16654395359407204
1107	34-35	0.2224843287715288
1107	36-37	0.20638002338427697
1107	38-39	0.1739759960806211
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2216	6	0.26556126814131176
2216	7	0.1659206252345058
2216	8	0.10477508262457746
2216	9	0.23075324113143836
2216	10-11	0.09198255683053702
2216	12-13	-0.07098993466625103
2216	14-15	-0.04750191255924108
2216	16-17	-0.029854891302868225
2216	18-19	0.07493242411798207
2216	20-21	0.12873055067296946
2216	22-23	0.18033599336246908
2216	24-25	0.26099726508056875
2216	26-27	0.3495210653759244
2216	28-29	0.3684076354598602
2216	30-31	0.3506638742311594
2216	32-33	0.37447647625374714
2216	34-35	0.3720556259777865
2216	36-37	0.4320901579018326
2216	38-39	0.45244912797318904
2216	40-41	0.4619518106692482
2216	42-43	0.5315465775933603
2216	44-45	0.5724443442907798
2216	46-47	0.5192268588144486
2216	48-49	0.44041453755985316
2216	50-51	0.4064997151563503
2216	52-53	0.4888086448225124
2216	54-55	0.452030678302485
2216	56-57	0.4426788531186787
2216	58-59	0.3637220875101832
2216	60-61	0.3508962043717645
2216	62-63	0.3246436263680579
2216	64-65	0.2934710001397747
2216	66-67	0.2882237691853611
2216	68-69	0.27565583033845087
2216	70-71	0.22998139319153665
2216	72-73	0.20130567037114133
2216	74-75	0.19978541377441772
2216	76-77	0.10225279448876279
2216	78-79	0.09356733607911494
2216	80-81	0.07283671731116215
2216	82-83	-0.03420123860930602
2216	84-85	-0.04139971771089179
2216	86-87	-0.06627564110615225
2216	88-89	-0.13133612572052655
2216	90-91	-0.1423045967387182
2216	92-93	-0.09575633306493359
2216	94-95	-0.09270702944677467
2216	96-97	-0.09679042867924181
2216	98-99	-0.031991478484517444
2216	100	0.3508649989425372
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	363.0
6	3190.0
7	9994.0
8	18795.0
9	29668.0
10	40346.0
11	47294.0
12	49532.0
13	51801.0
14	56670.0
15	63953.0
16	74529.0
17	86723.0
18	101691.0
19	116032.0
20	131949.0
21	149501.0
22	167319.0
23	193076.0
24	257411.0
25	317096.0
26	275421.0
27	518356.0
28	377111.0
29	619187.0
30	560915.0
31	696642.0
32	936686.0
33	1611140.0
34	2043484.0
35	4825733.0
36	2091324.0
37	896168.0
38	148515.0
39	4603.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.127567449120574	32.91630916249401	25.807848443843028	26.148274944542386
2	12.554172369345812	36.10449489675424	27.430542079369914	23.910790654530036
3	11.004414977746988	39.08057424018319	24.976808576760522	24.9382022053093
4	9.847140052636465	38.73073245289485	24.9286018296256	26.493525664843087
5	9.578676672151268	38.00027873527029	25.931747190410505	26.48929740216793
6	9.56757333243491	35.042860342846325	27.208350254685314	28.181216070033454
7	12.727740305926085	39.99813796294626	22.6063476683819	24.667774062745753
8	11.631876638985299	37.12226832463884	19.957798182575473	31.288056853800388
9	9.471883162915757	36.12942899844977	21.018187277208828	33.38050056142565
10-11	11.94655467134452	51.528387584624255	20.23864165836163	16.286416085669593
12-13	11.470045324656837	52.41368856934924	19.866253692937608	16.25001241305632
14-15	12.519988288295721	51.136072305262324	19.498505164275432	16.84543424216652
16-17	12.531141879096225	50.887884113526496	20.012107193764468	16.56886681361281
18-19	12.878526597400617	49.385425436142825	21.001002919583787	16.735045046872767
20-21	12.060346161735573	48.81219472588537	21.84780072887207	17.27965838350699
22-23	12.165859914810396	49.34385475433389	22.47865799726039	16.011627333595317
24-25	12.11535827355767	49.347219979889864	22.042328836028712	16.49509291052376
26-27	13.380559176641734	49.44695690000398	21.181259930086274	15.991223993268013
28-29	12.903624804798527	50.27654146778988	21.871573606285235	14.948260121126353
30-31	13.168452010931247	50.09638380681479	21.6756791532213	15.059485029032658
32-33	13.571305365588717	50.24976071604844	21.136954871778006	15.041979046584839
34-35	13.63660872904236	50.43015524092669	20.99333876900178	14.939897261029172
36-37	13.946751979440256	50.28570266021006	20.729467464309042	15.038077896040637
38-39	14.306495578251985	50.32278114602317	20.64242607452725	14.728297201197602
40-41	14.408873887930651	50.56349437621041	20.669332872066956	14.35829886379199
42-43	14.54902954341437	50.621231299408755	20.842474276461274	13.9872648807156
44-45	14.714171214778313	50.10337962482404	20.8813753234668	14.30107383693085
46-47	14.812606557454483	49.811934753589114	21.122344390050745	14.253114298905656
48-49	14.64511200380468	50.02503693794863	21.34449466501806	13.98535639322863
50-51	14.921376792892922	49.85870602983834	21.484872522432983	13.735044654835765
52-53	15.014604029488615	49.884627455689255	21.61149667898821	13.489271835833922
54-55	14.972531232538053	50.07513598657511	21.57249325195982	13.379839528927013
56-57	14.864119750557231	50.23188712442154	21.233533652471326	13.67045947254991
58-59	14.62559225901294	50.22885961550697	21.471459970182323	13.67408815529777
60-61	14.555435125541367	50.179775277062376	21.69141046297177	13.57337913442448
62-63	14.673428071880984	49.7067055204943	21.829948244058773	13.789918163565936
64-65	14.486867346251813	49.700117077033816	21.86496550145625	13.948050075258116
66-67	14.210360653334503	49.57077355114413	22.115825270073007	14.10304052544836
68-69	14.187732862377544	49.52392296050892	21.92357323782039	14.364770939293145
70-71	14.052771786120022	49.5170729855714	22.03497449965761	14.395180728650963
72-73	14.130140900888616	49.778157343280746	21.94033691629687	14.151364839533764
74-75	14.118472160283217	50.012962257565164	21.74454487566007	14.124020706491553
76-77	13.74131915348156	50.409847097366445	21.790715235227445	14.058118513924553
78-79	13.443268450904991	50.790116509747385	21.61505016021997	14.151564879127655
80-81	13.19562670051741	51.35824144280561	21.364381594477795	14.081750262199188
82-83	12.85733354036548	51.90435285652715	21.45832406796197	13.779989535145399
84-85	12.581084833573303	52.652483790289104	21.305861724993626	13.460569651143969
86-87	12.251426147441117	53.194350600338915	21.110197472784957	13.444025779435012
88-89	12.108842159218568	53.739069017954655	20.91226079969374	13.239828023133033
90-91	12.064361410924867	54.231858374848194	20.647702996012978	13.056077218213957
92-93	12.04530398392406	54.54779529159212	20.348914531942317	13.057986192541504
94-95	12.126160650032594	54.64943624990599	20.083224526875796	13.14117857318562
96-97	12.264004592276931	54.524493386138985	19.96837773219165	13.243124289392435
98-99	12.473246047585471	54.19136338881311	19.850101040452035	13.485289523149389
100	12.859022383205065	53.410900794266624	19.76562336832582	13.964453454202493
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1848669.0
1	1724435.5
2	972610.0
3	210001.0
4	72251.0
5	67459.5
6	59469.0
7	47047.5
8	36463.5
9	31990.0
10	33544.5
11	39185.0
12	49119.0
13	64158.5
14	85045.0
15	111901.5
16	143058.0
17	177254.5
18	214146.0
19	251448.0
20	284926.0
21	314723.5
22	341326.5
23	362556.0
24	378487.0
25	388485.5
26	391610.0
27	388451.0
28	379205.0
29	364130.0
30	343709.5
31	321574.0
32	298615.0
33	274973.5
34	252914.5
35	231836.0
36	212825.5
37	195214.5
38	178811.5
39	165451.0
40	154994.5
41	149949.0
42	151579.5
43	161594.0
44	183428.5
45	215136.0
46	252330.0
47	290430.5
48	325217.0
49	355125.0
50	384548.5
51	415748.0
52	445165.5
53	464643.0
54	462844.5
55	430819.5
56	367745.5
57	286965.5
58	205602.5
59	141274.0
60	90415.0
61	51924.0
62	32306.0
63	21772.5
64	16046.5
65	12741.0
66	10643.0
67	9157.5
68	7916.5
69	6886.5
70	6007.5
71	5394.0
72	4884.0
73	4405.0
74	4045.0
75	3760.5
76	3515.5
77	3311.0
78	3122.0
79	2926.5
80	2750.5
81	2651.0
82	2567.0
83	2483.0
84	2390.0
85	2289.5
86	2235.5
87	2164.0
88	2065.5
89	2020.5
90	2019.0
91	1976.5
92	1917.0
93	1789.0
94	1730.0
95	1711.5
96	1560.0
97	1409.5
98	1228.5
99	1014.5
100	823.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014249757387067968
2	4.495730166047802E-4
3	0.0
4	0.0
5	0.0
6	0.0042168810797992675
7	4.3250062356915567E-4
8	0.0
9	0.0
10-11	3.7843804562301116E-4
12-13	3.129938723197837E-5
14-15	3.6421105142665734E-4
16-17	1.7926012687405793E-4
18-19	1.8495092455259946E-4
20-21	5.406257794614446E-5
22-23	8.251656633885206E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	1.9633251990968246E-4
32-33	5.690797678541521E-5
34-35	1.5080613848135032E-4
36-37	1.5080613848135032E-4
38-39	6.032245539254013E-4
40-41	0.0017897558699013084
42-43	0.0010869423566014307
44-45	5.747705655326936E-4
46-47	7.739484842816469E-4
48-49	0.001092633154279972
50-51	0.0
52-53	1.365791442849965E-4
54-55	5.12171791068737E-4
56-57	5.804613632112352E-4
58-59	5.861521608897767E-4
60-61	7.398036982103978E-5
62-63	2.475496990165562E-4
64-65	2.1625031178457784E-4
66-67	2.0486871642749478E-4
68-69	1.166613524101012E-4
70-71	6.373693399966505E-4
72-73	5.12171791068737E-4
74-75	3.32911664194679E-4
76-77	3.755926467837404E-4
78-79	7.796392819601884E-4
80-81	0.0010670245647265353
82-83	4.4672761776550944E-4
84-85	7.028135132998778E-4
86-87	0.001354409847492882
88-89	0.0012206761020471563
90-91	0.001189376714815178
92-93	7.511852935674808E-4
94-95	7.14195108656961E-4
96-97	6.715141260678996E-4
98-99	3.357570630339498E-4
100	9.674356053520586E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.7572229E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.04499191488107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.34880922448924	65.60920404702733
2	6.699924044324041	9.519920991186375
3	0.6987881307162759	1.4893619129085804
4	0.09834637136356333	0.27948068633528994
5	0.041830411930336285	0.14859206386934432
6	0.02301173602161527	0.09809211597618207
7	0.01625518474889365	0.08083946283420333
8	0.011024129752694508	0.06265673673190252
9	0.007817323341459211	0.04998435062309827
>10	0.0461168472122393	0.5825003893163339
>50	0.0039034267521500313	0.1909374421662778
>100	0.002508573701114321	0.376706025946995
>500	4.615792867381098E-4	0.22845896446819242
>1k	6.015387460210482E-4	0.8705036621877869
>5k	1.9247283638388423E-4	0.9274433294855406
>10k+	4.090047773157539E-4	19.485317818936593
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	895679	5.097127973918392	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	254599	1.4488713981589927	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	211903	1.205897100475984	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	195681	1.1135809805346835	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	131026	0.7456424566285814	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	110044	0.6262381397374231	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	106839	0.6079991331776976	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	104316	0.5936412506347373	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	83001	0.4723418981166248	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79778	0.4540004571986855	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79349	0.45155910499459123	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	75843	0.43160716833362467	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	75449	0.4293649940482792	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70601	0.40177600690271	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63754	0.3628111151977362	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58621	0.3336002507137825	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56240	0.32005046144117516	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54151	0.30816238509070193	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50276	0.28611054408635356	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41363	0.23538846437751296	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38586	0.21958511922420312	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37512	0.21347320251744956	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36316	0.20666700849391392	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35034	0.19937140587002367	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30132	0.17147511564981313	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29789	0.16952317204607337	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29694	0.16898254626661194	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27038	0.15386778763240566	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24156	0.13746690872284897	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22242	0.12657472196612052	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22216	0.12642676122647845	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20989	0.119444152474908	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20343	0.11576789717457017	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18259	0.10390827481248964	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0017186208989195394	0.0	0.0	0.0	0.0
2	0.004159973103013852	0.0	0.0	1.7072393035624563E-5	0.0
3	0.0067492860467502445	0.0	0.0	5.1217179106873695E-5	5.690797678541522E-6
4	0.010493830919230566	0.0	0.0	5.6907976785415216E-5	5.690797678541522E-6
5	0.01769838078026413	0.0	0.0	6.259877446395674E-5	5.690797678541522E-6
6	0.030548201938410888	0.0	0.0	7.398036982103978E-5	5.690797678541522E-6
7	0.048940860035457084	0.0	0.0	7.398036982103978E-5	5.690797678541522E-6
8	0.11696865548474243	0.0	0.0	7.398036982103978E-5	5.690797678541522E-6
9	0.3675401680686042	0.0	0.0	7.398036982103978E-5	5.690797678541522E-6
10-11	0.8907179618476404	0.0	0.0	7.398036982103978E-5	5.690797678541522E-6
12-13	1.5128388094646388	0.0	0.0	7.96711674995813E-5	5.690797678541522E-6
14-15	2.149593543312007	0.0	0.0	9.105276285666433E-5	5.690797678541522E-6
16-17	2.775328047454879	0.0	0.0	1.1381595357083043E-4	5.690797678541522E-6
18-19	3.4138611555767913	0.0	0.0	1.4226994196353803E-4	5.690797678541522E-6
20-21	4.21874481603899	0.0	0.0	1.5934233499916259E-4	5.690797678541522E-6
22-23	5.146760265871791	0.0	5.690797678541522E-6	1.5934233499916259E-4	5.690797678541522E-6
24-25	6.118967605077307	0.0	5.690797678541522E-6	1.650331326777041E-4	5.690797678541522E-6
26-27	7.145379792170931	0.0	5.690797678541522E-6	1.650331326777041E-4	5.690797678541522E-6
28-29	8.303044536922435	0.0	5.690797678541522E-6	1.735693291955164E-4	5.690797678541522E-6
30-31	9.609008623777894	0.0	5.690797678541522E-6	1.7641472803478717E-4	5.690797678541522E-6
32-33	11.071068445556907	0.0	5.690797678541522E-6	1.8210552571332867E-4	5.690797678541522E-6
34-35	12.436677782881159	0.0	5.690797678541522E-6	1.963325199096825E-4	5.690797678541522E-6
36-37	13.497058341317997	0.0	5.690797678541522E-6	2.0486871642749478E-4	5.690797678541522E-6
38-39	14.328148125089879	0.0	5.690797678541522E-6	2.390135024987439E-4	5.690797678541522E-6
40-41	15.1705654416409	0.0	5.690797678541522E-6	2.674674908914515E-4	5.690797678541522E-6
42-43	15.872220877613193	0.0	5.690797678541522E-6	3.21530068837596E-4	5.690797678541522E-6
44-45	16.396272208835885	0.0	5.690797678541522E-6	3.755926467837404E-4	5.690797678541522E-6
46-47	16.84286893825479	0.0	5.690797678541522E-6	4.0973743285498956E-4	5.690797678541522E-6
48-49	17.253420155177807	0.0	5.690797678541522E-6	4.609546119618632E-4	5.690797678541522E-6
50-51	17.668748796751967	0.0	5.690797678541522E-6	5.207079875865492E-4	5.690797678541522E-6
52-53	18.109933577578573	0.0	5.690797678541522E-6	5.747705655326936E-4	5.690797678541522E-6
54-55	18.56554453052029	0.0	5.690797678541522E-6	6.629779295500872E-4	5.690797678541522E-6
56-57	19.044072325713486	0.0	5.690797678541522E-6	6.971227156213364E-4	5.690797678541522E-6
58-59	19.552621924059835	0.0	5.690797678541522E-6	7.085043109784194E-4	5.690797678541522E-6
60-61	20.0744253901995	0.0	5.690797678541522E-6	7.198859063355025E-4	5.690797678541522E-6
62-63	20.572225071731083	0.0	5.690797678541522E-6	7.597214900852931E-4	5.690797678541522E-6
64-65	21.051179676750174	2.845398839270761E-6	5.690797678541522E-6	7.910208773172715E-4	5.690797678541522E-6
66-67	21.496956931303366	1.1381595357083044E-5	5.690797678541522E-6	8.849190390132066E-4	5.690797678541522E-6
68-69	21.901737679380346	1.1381595357083044E-5	5.690797678541522E-6	9.475178134771633E-4	5.690797678541522E-6
70-71	22.285297442914043	1.1381595357083044E-5	5.690797678541522E-6	9.98734992584037E-4	5.690797678541522E-6
72-73	22.657185380409054	1.1381595357083044E-5	5.690797678541522E-6	0.001021498183298203	5.690797678541522E-6
74-75	23.01691492866386	1.1381595357083044E-5	5.690797678541522E-6	0.0010727153624050768	5.690797678541522E-6
76-77	23.368836702503707	1.1381595357083044E-5	5.690797678541522E-6	0.0011495411310653873	5.690797678541522E-6
78-79	23.714444536319213	1.1381595357083044E-5	5.690797678541522E-6	0.0012519754892791348	5.690797678541522E-6
80-81	24.053678676734748	1.1381595357083044E-5	5.690797678541522E-6	0.0014539988068673587	5.690797678541522E-6
82-83	24.392471780330204	1.1381595357083044E-5	5.690797678541522E-6	0.0015308245755276692	5.690797678541522E-6
84-85	24.73556428157179	1.1381595357083044E-5	5.690797678541522E-6	0.0015621239627596475	5.690797678541522E-6
86-87	25.083468352250588	1.1381595357083044E-5	5.690797678541522E-6	0.001582041754634543	5.690797678541522E-6
88	25.34289190062342	1.1381595357083044E-5	5.690797678541522E-6	0.001593423349991626	5.690797678541522E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCTCA	2100	0.0	68.26399	2
AGCTCAC	2445	0.0	59.977295	3
CCAGCTC	2710	0.0	54.81406	1
GCTCACC	3315	0.0	44.803783	4
TTGCTGA	5065	0.0	41.106987	9
GCTTGCT	1280	0.0	37.45299	7
CTTGCTG	3415	0.0	36.470905	8
CCTGCTG	4175	0.0	32.87137	8
CTGCTGA	5290	0.0	32.250935	9
ACTTGCC	1040	0.0	32.08752	3
GCCTGCT	1290	0.0	31.697563	7
TGAAAAA	16585	0.0	31.433317	1
TGCCAAA	2115	0.0	30.000916	6
ATTGCGC	325	0.0	28.923964	2
CATTCCG	2500	0.0	28.575653	16-17
ATTCCGC	2545	0.0	28.070385	16-17
GCCGCCA	2465	0.0	27.74269	34-35
TCGCGAC	850	0.0	27.094944	6
ATGTGCC	545	0.0	26.73476	3
GACACGC	900	0.0	26.63415	2
>>END_MODULE