FastQCFastQC Report
Thu 26 May 2016
SRR1248479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22116506
Sequences flagged as poor quality0
Sequence length100
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15134556.8431017087418775No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4419711.9983762353782284No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3434171.5527633524029518No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3273311.4800303447569882No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2392501.0817712345702346No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1995880.902439110409212No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1953090.8830915697081628No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1904840.8612752846222635No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1526780.6903350827657859No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1497160.6769423705534681No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1473880.6664162955938882No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1418480.6413671309563997No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1319620.5966674844570837No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1247660.564130699487523No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1113480.5034610801543427No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1044390.47222196851528No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1013210.45812390076443354No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA984760.44526020520601217No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA912110.41241143605594843No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA727290.3288448907797642No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA712690.3222434863807149No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA701050.3169804489009249No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670390.30311749966292145No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650680.2942056037242049No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540350.24431978541275914No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA537000.24280507960886769No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA513650.23224735408025118No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA485350.21945148116976526No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA448570.20282136789599586No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA431800.1952387958568139No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA415110.18769239589653086No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA381200.17235995595325954No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360220.16287382826202296No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA359710.16264323125904245No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA339540.15352334586665725No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311450.1408224246632809No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA308480.13947953623415923No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA297430.13448326783624864No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280510.12683287314913125No Hit
TAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274190.12397527891611813No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA265260.11993757060902838No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252560.11419525308382797No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250940.11346276848612526No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250190.11312365524644805No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA243250.10998572740196846No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239070.10809573627950093No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA235090.10629617535428064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTGA53300.040.4743659
TGAAAAA243900.037.157861
CTGCTGA57000.034.0541279
CCTGCTG45000.033.8395928
GCCTGCT13900.033.4745677
GCTTGCT13400.032.6191567
CTTGCTG38550.032.0644658
TACCCGA102950.030.770746
CCCGACG117150.030.1695048
TGCCAAA23450.029.663566
GTACGAC9300.029.3122716
ACCCGAC111550.029.2404797
TCGCGAC7500.028.82716
GAAAACA92850.028.5530571
CTACCCG104300.028.3896885
ATGCCAA14800.028.2639395
AGTTGCC5250.027.7527353
ACTTGCC11100.027.5228753
GACACGC9400.027.5004752
TTGCCAA12750.026.9102085
Produced by FastQC (version 0.11.5)