##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248479_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22116506
Sequences flagged as poor quality	0
Sequence length	100
%GC	24
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.200133104207328	31.0	30.0	33.0	16.0	34.0
2	29.925649015264888	31.0	30.0	34.0	23.0	34.0
3	30.5007259736235	31.0	30.0	34.0	26.0	34.0
4	34.14078173107452	35.0	35.0	37.0	30.0	37.0
5	33.912131916316255	35.0	35.0	37.0	28.0	37.0
6	33.58677943975418	35.0	35.0	37.0	28.0	37.0
7	33.781422119750744	35.0	35.0	37.0	28.0	37.0
8	33.7785193556342	35.0	35.0	37.0	28.0	37.0
9	35.033995514481354	37.0	34.0	39.0	28.0	39.0
10-11	35.86585928175092	38.5	35.0	39.0	31.0	39.0
12-13	36.18241672079668	39.0	35.0	39.0	31.0	39.0
14-15	37.21592895369639	39.5	36.5	41.0	31.0	41.0
16-17	36.92310966750354	39.0	36.0	40.5	31.0	41.0
18-19	36.14468447231222	38.0	35.5	40.0	30.0	41.0
20-21	35.486396788896045	37.0	34.0	39.5	29.5	41.0
22-23	34.94783054791747	36.0	34.0	39.0	28.0	40.0
24-25	34.54380052165564	35.0	33.5	39.0	27.0	40.0
26-27	34.298005864940876	35.0	33.0	39.0	26.0	40.0
28-29	34.161882012466165	35.0	33.0	39.0	25.0	40.0
30-31	34.013652857282246	35.0	33.0	38.5	25.0	40.0
32-33	33.87896162259988	35.0	33.0	38.0	25.0	40.0
34-35	33.721456589933325	35.0	33.0	38.0	25.0	40.0
36-37	33.46521392212676	35.0	32.5	38.0	25.0	40.0
38-39	33.24841717765003	35.0	32.0	38.0	25.0	40.0
40-41	33.02465027703743	35.0	31.0	38.0	25.0	40.0
42-43	32.84027707631576	35.0	31.0	37.5	24.5	40.0
44-45	32.56242588680147	35.0	30.0	37.0	24.0	39.5
46-47	32.48307490342281	35.0	30.0	37.0	23.5	39.0
48-49	32.55968492491536	35.0	30.0	37.0	24.0	39.0
50-51	32.469194320296346	35.0	30.0	37.0	24.0	39.0
52-53	32.39823948683395	35.0	30.0	37.0	24.0	39.0
54-55	32.27166438496207	35.0	30.0	36.0	23.5	39.0
56-57	32.562692859351294	35.0	30.5	36.0	24.5	39.0
58-59	32.70478682301807	35.0	31.0	36.0	25.0	39.0
60-61	32.574270660112404	35.0	31.0	36.0	25.0	39.0
62-63	32.37767636081395	35.0	30.5	35.0	25.0	39.0
64-65	32.206734825112065	35.0	30.0	35.0	25.0	38.5
66-67	32.0349121375682	34.0	30.0	35.0	25.0	38.0
68-69	31.9010744509101	34.0	30.0	35.0	25.0	37.0
70-71	31.781570606134622	34.0	30.0	35.0	25.0	37.0
72-73	31.682932398996478	34.0	30.0	35.0	25.0	36.0
74-75	31.58083835665543	34.0	30.0	35.0	24.0	36.0
76-77	31.44176238778404	34.0	30.0	35.0	24.0	36.0
78-79	31.30913361721784	34.0	30.0	35.0	24.0	35.5
80-81	31.221828845840296	34.0	30.0	35.0	24.0	35.0
82-83	31.12757042183788	34.0	30.0	35.0	23.5	35.0
84-85	31.043148791224077	34.0	30.0	35.0	23.0	35.0
86-87	30.92497325300841	34.0	30.0	35.0	23.0	35.0
88-89	30.801493689826053	34.0	30.0	35.0	20.5	35.0
90-91	30.621117345569864	34.0	30.0	35.0	20.0	35.0
92-93	30.42512424883026	34.0	29.0	35.0	19.5	35.0
94-95	30.234414717225224	34.0	29.0	35.0	18.5	35.0
96-97	29.984494906202634	34.0	29.0	35.0	16.5	35.0
98-99	29.67425512420452	34.0	28.0	35.0	14.0	35.0
100	28.224412843511537	33.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32778566016463984
1101	2	0.34712990592989357
1101	3	0.3519521053709802
1101	4	0.21461519745171387
1101	5	0.1977128593934907
1101	6	0.13965992417010398
1101	7	0.21436183375763562
1101	8	0.21439760868603486
1101	9	0.24389096719858827
1101	10-11	0.3928103764139905
1101	12-13	0.48700562862392616
1101	14-15	0.6402211589568978
1101	16-17	0.6678342906975985
1101	18-19	0.6354265339326801
1101	20-21	0.5377788838285582
1101	22-23	0.5463319420751063
1101	24-25	0.5745345885783024
1101	26-27	0.5913619730799198
1101	28-29	0.6416616782573072
1101	30-31	0.6062469765942922
1101	32-33	0.6288987708522953
1101	34-35	0.6606692833835979
1101	36-37	0.6523616392716676
1101	38-39	0.6343655089964457
1101	40-41	0.6565301943635973
1101	42-43	0.6416567369995647
1101	44-45	0.6067056461704396
1101	46-47	0.6686574002951886
1101	48-49	0.6880872725433278
1101	50-51	0.6423976049493874
1101	52-53	0.6200676469806155
1101	54-55	0.5873970998879301
1101	56-57	0.5529713697035419
1101	58-59	0.5456963145375937
1101	60-61	0.558884752065957
1101	62-63	0.506820091032786
1101	64-65	0.5284473869630588
1101	66-67	0.54145692090664
1101	68-69	0.5239557897090528
1101	70-71	0.4954845280290989
1101	72-73	0.4798532832767535
1101	74-75	0.46574109859638924
1101	76-77	0.47604696777098354
1101	78-79	0.49039804301403933
1101	80-81	0.4839473974569337
1101	82-83	0.49990925981301615
1101	84-85	0.4567460342440981
1101	86-87	0.44710552986458296
1101	88-89	0.43537511558532316
1101	90-91	0.41717729149576854
1101	92-93	0.36578677933597703
1101	94-95	0.35966776792395194
1101	96-97	0.3196673012112612
1101	98-99	0.33401299038416354
1101	100	0.3578171946898472
1102	1	0.19824133765317598
1102	2	0.2588363686449533
1102	3	0.22900822055019532
1102	4	0.18362540961430085
1102	5	0.16962897834120128
1102	6	0.10376567443971396
1102	7	0.11712301576147155
1102	8	0.13625411159861045
1102	9	0.08446810014525141
1102	10-11	0.24912884831699955
1102	12-13	0.33191575589759026
1102	14-15	0.4269309346276202
1102	16-17	0.44315844510995817
1102	18-19	0.40707742997667395
1102	20-21	0.3377221339351948
1102	22-23	0.3450308178999535
1102	24-25	0.3045231191949398
1102	26-27	0.30545548007689405
1102	28-29	0.34649164621421846
1102	30-31	0.3333263744963162
1102	32-33	0.2876638510950684
1102	34-35	0.3554869139842225
1102	36-37	0.31884776833283013
1102	38-39	0.27086820312202775
1102	40-41	0.27154318639521335
1102	42-43	0.3053541930816479
1102	44-45	0.2776111221831101
1102	46-47	0.3004919503404011
1102	48-49	0.3144761949313306
1102	50-51	0.34602774649325596
1102	52-53	0.34008457564760164
1102	54-55	0.32608577395518523
1102	56-57	0.3254921460961242
1102	58-59	0.3900628810387943
1102	60-61	0.36428354366185545
1102	62-63	0.3653491412454173
1102	64-65	0.40027763228791713
1102	66-67	0.3661053618299377
1102	68-69	0.34969485587528126
1102	70-71	0.3750230269391217
1102	72-73	0.37086923752705303
1102	74-75	0.356968762515784
1102	76-77	0.35800520586548146
1102	78-79	0.3743768180765912
1102	80-81	0.3668275492126689
1102	82-83	0.40135179366794205
1102	84-85	0.34440975302636545
1102	86-87	0.36413811778932725
1102	88-89	0.33477906944090563
1102	90-91	0.3512132947380451
1102	92-93	0.34287282699212795
1102	94-95	0.3104806465993164
1102	96-97	0.3403804972827942
1102	98-99	0.2805418549759118
1102	100	0.2211866781751901
1103	1	0.196643813159195
1103	2	0.20502720525435691
1103	3	0.21856955469909067
1103	4	0.1763040426983551
1103	5	0.1596818808172813
1103	6	0.1154083338054619
1103	7	0.14443597690577548
1103	8	0.20839649613360933
1103	9	0.17117554601440332
1103	10-11	0.25654716791791543
1103	12-13	0.3166204178637031
1103	14-15	0.4036218120332009
1103	16-17	0.3919899361361985
1103	18-19	0.33233264228699255
1103	20-21	0.2913436241694498
1103	22-23	0.24962844134532958
1103	24-25	0.2574530709265801
1103	26-27	0.1956464256799606
1103	28-29	0.24828292163279286
1103	30-31	0.2102174182916201
1103	32-33	0.2563927435168054
1103	34-35	0.2853726778445207
1103	36-37	0.2420248651863659
1103	38-39	0.20442850011162506
1103	40-41	0.16662523641061
1103	42-43	0.2024788028838742
1103	44-45	0.15663343822538423
1103	46-47	0.1970327544779451
1103	48-49	0.25033616167953454
1103	50-51	0.2584870080958055
1103	52-53	0.26051571493550796
1103	54-55	0.24849865823180295
1103	56-57	0.27091510343629466
1103	58-59	0.286074073877721
1103	60-61	0.2920261748910704
1103	62-63	0.29554694677056403
1103	64-65	0.2935806496152793
1103	66-67	0.33442544215623826
1103	68-69	0.3349335769225483
1103	70-71	0.34597839487230075
1103	72-73	0.3561751622472613
1103	74-75	0.3659328652002962
1103	76-77	0.36398373687954333
1103	78-79	0.3729493380312725
1103	80-81	0.3794975444138693
1103	82-83	0.4025819969141793
1103	84-85	0.36918856555996626
1103	86-87	0.3943699427295364
1103	88-89	0.35106261844576636
1103	90-91	0.32090583865998923
1103	92-93	0.3179422104053593
1103	94-95	0.3329048632208931
1103	96-97	0.30981034505298766
1103	98-99	0.2922405755687443
1103	100	0.22525585907665757
1104	1	0.06694990128815448
1104	2	0.16522698379639067
1104	3	0.14293933934303737
1104	4	0.08829687920048457
1104	5	0.09362805036738564
1104	6	0.10342820415461773
1104	7	0.14570032929724164
1104	8	0.141004237707115
1104	9	0.10768578483859415
1104	10-11	0.11222338440354918
1104	12-13	0.16844591697435618
1104	14-15	0.22396427741077218
1104	16-17	0.20220120774605022
1104	18-19	0.19302998752802125
1104	20-21	0.15873687853157037
1104	22-23	0.15861028820985013
1104	24-25	0.15458199718334953
1104	26-27	0.1312603355052957
1104	28-29	0.07125641864077181
1104	30-31	0.10311637913079608
1104	32-33	0.12598293291399898
1104	34-35	0.12585392168945475
1104	36-37	0.13107018938480763
1104	38-39	0.10406281463923506
1104	40-41	0.06928269512795282
1104	42-43	0.08978294386883334
1104	44-45	0.06826296040105007
1104	46-47	0.13678314739189545
1104	48-49	0.14941759767610563
1104	50-51	0.1754031450379827
1104	52-53	0.16168435542704884
1104	54-55	0.17522571967999312
1104	56-57	0.1683638183312013
1104	58-59	0.16425451362353982
1104	60-61	0.1779912672681334
1104	62-63	0.17082404088569803
1104	64-65	0.19632525687882918
1104	66-67	0.1792403893143515
1104	68-69	0.2248086752487204
1104	70-71	0.21571642721378836
1104	72-73	0.19156466224136182
1104	74-75	0.2170325720475006
1104	76-77	0.21200360267322793
1104	78-79	0.2532927029480945
1104	80-81	0.2448442738419523
1104	82-83	0.2515329486269984
1104	84-85	0.24023268513603568
1104	86-87	0.24257122929279618
1104	88-89	0.2810621104684472
1104	90-91	0.26856514472715176
1104	92-93	0.28024561352865973
1104	94-95	0.24211768001043765
1104	96-97	0.22477669456995386
1104	98-99	0.22246554952667807
1104	100	0.24267920841491986
1105	1	0.1137690893574721
1105	2	0.16450697376535928
1105	3	0.1372809687936467
1105	4	0.1572723229881987
1105	5	0.10705178497015311
1105	6	0.04644405683981745
1105	7	0.08868893594218719
1105	8	0.1003453181439724
1105	9	0.05508546580286833
1105	10-11	0.16315924271057725
1105	12-13	0.22867667810738368
1105	14-15	0.2556729017016295
1105	16-17	0.2562110309393404
1105	18-19	0.20813300930544898
1105	20-21	0.18574812246415462
1105	22-23	0.1693324021746534
1105	24-25	0.16258971502930564
1105	26-27	0.09698861582212714
1105	28-29	0.09521268645911363
1105	30-31	0.08212647515386351
1105	32-33	0.09314762650934227
1105	34-35	0.14430577560735003
1105	36-37	0.1763688032307087
1105	38-39	0.1510424007554576
1105	40-41	0.10509244395022677
1105	42-43	0.1255836407924633
1105	44-45	0.0946088645759886
1105	46-47	0.08543101006007703
1105	48-49	0.14387147466314332
1105	50-51	0.16885399528573686
1105	52-53	0.2201545010583672
1105	54-55	0.2070786826967037
1105	56-57	0.20797598402069895
1105	58-59	0.21457950828095562
1105	60-61	0.2204846989688818
1105	62-63	0.24282701958377828
1105	64-65	0.227562365847092
1105	66-67	0.20285711734445044
1105	68-69	0.21885473862047533
1105	70-71	0.257619187462776
1105	72-73	0.25784576135067994
1105	74-75	0.2556301933488925
1105	76-77	0.21728149699014665
1105	78-79	0.24296250150480958
1105	80-81	0.2319020977518953
1105	82-83	0.27700433357430043
1105	84-85	0.2618956944862454
1105	86-87	0.24824540859510336
1105	88-89	0.2629521422954326
1105	90-91	0.23734413101800556
1105	92-93	0.22313088827069194
1105	94-95	0.19376595533539032
1105	96-97	0.18671860186728395
1105	98-99	0.19603211870776605
1105	100	0.2722278430430016
1106	1	-0.016641301042120915
1106	2	0.017262553599287855
1106	3	0.07682906280608748
1106	4	0.04915131789807958
1106	5	-0.00637121241243932
1106	6	-0.054527066806386415
1106	7	0.005660607858338551
1106	8	0.049901142313807156
1106	9	-0.014777620746677655
1106	10-11	0.04783795218104103
1106	12-13	0.0885381877395588
1106	14-15	0.10344014882335273
1106	16-17	0.07813948308666596
1106	18-19	0.0327706979642457
1106	20-21	0.05075924903715645
1106	22-23	0.04294404476815572
1106	24-25	0.04008953632536105
1106	26-27	0.04197278380257785
1106	28-29	0.07739022803838935
1106	30-31	0.04604106020627796
1106	32-33	0.01317037679683608
1106	34-35	0.10332480679635125
1106	36-37	0.10804061013960364
1106	38-39	0.07643100535012337
1106	40-41	0.08771732681846345
1106	42-43	0.09919811997778538
1106	44-45	0.06118460022148042
1106	46-47	0.03401543060691381
1106	48-49	0.05501225966098389
1106	50-51	0.1184117827543858
1106	52-53	0.11068997366785283
1106	54-55	0.14014711856212614
1106	56-57	0.14901140716457917
1106	58-59	0.16805791118682123
1106	60-61	0.1455382347000196
1106	62-63	0.15587778906726157
1106	64-65	0.15622199500665346
1106	66-67	0.13023551519468413
1106	68-69	0.15600202873108415
1106	70-71	0.13605045848175124
1106	72-73	0.11988279788294776
1106	74-75	0.11306445319418401
1106	76-77	0.10318988386973871
1106	78-79	0.11459349287349951
1106	80-81	0.12033929234722152
1106	82-83	0.07601799215090921
1106	84-85	0.06494576659483897
1106	86-87	0.12472094988141791
1106	88-89	0.1273131415021176
1106	90-91	0.10077062402433512
1106	92-93	0.05960036936697577
1106	94-95	0.10198821251433188
1106	96-97	0.09993337554627502
1106	98-99	0.11562301728787716
1106	100	0.13154831530574285
1107	1	-0.018909834223325817
1107	2	0.020752393252745804
1107	3	0.03425945344648085
1107	4	0.06272846545474664
1107	5	0.036131604775242465
1107	6	0.02601129420910553
1107	7	0.024814198945229293
1107	8	-0.0018008632660624357
1107	9	-0.05275256358994085
1107	10-11	0.06137621529048687
1107	12-13	0.07799596970909306
1107	14-15	0.12414664606228598
1107	16-17	0.11144970581257496
1107	18-19	0.06448596342491442
1107	20-21	0.07990454788143353
1107	22-23	0.08190954600329547
1107	24-25	0.1380049256318827
1107	26-27	0.12062116641884302
1107	28-29	0.16815494597145175
1107	30-31	0.1335716961203488
1107	32-33	0.14033712061906556
1107	34-35	0.2011692185619296
1107	36-37	0.17983061807806422
1107	38-39	0.1811255710970059
1107	40-41	0.17608250952017102
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2216	2	0.4988036828040947
2216	3	0.21983597196658522
2216	4	0.2072126819197706
2216	5	0.28762591403302906
2216	6	0.36289111502089355
2216	7	0.24067845916776065
2216	8	0.1386933092130036
2216	9	0.27554201335714623
2216	10-11	0.13976605353774119
2216	12-13	0.009353638856651969
2216	14-15	0.04171019246865626
2216	16-17	0.08710369102891491
2216	18-19	0.20354454507275932
2216	20-21	0.21596400110517067
2216	22-23	0.27353456848194924
2216	24-25	0.3295932316034751
2216	26-27	0.40446240503024455
2216	28-29	0.40773774621838044
2216	30-31	0.4313079983948711
2216	32-33	0.43830458664429983
2216	34-35	0.40865329866254285
2216	36-37	0.48001888043037866
2216	38-39	0.47157126613334555
2216	40-41	0.499945758525584
2216	42-43	0.5574811854816488
2216	44-45	0.6102067900589603
2216	46-47	0.5408826000471407
2216	48-49	0.5160851974469836
2216	50-51	0.465467067605573
2216	52-53	0.5167555360639327
2216	54-55	0.49251035685726663
2216	56-57	0.43984545645505335
2216	58-59	0.41121114602007935
2216	60-61	0.3962994968475826
2216	62-63	0.36612392544402184
2216	64-65	0.361746605786216
2216	66-67	0.34272391445628614
2216	68-69	0.31193766720681637
2216	70-71	0.30272396641335675
2216	72-73	0.247913173545804
2216	74-75	0.2483942913594177
2216	76-77	0.19270662102287162
2216	78-79	0.18457421260092843
2216	80-81	0.09550228296953378
2216	82-83	0.03693820708220841
2216	84-85	-0.015339158399179098
2216	86-87	0.0028073267728210283
2216	88-89	-0.05846581567254461
2216	90-91	-0.06708063439381107
2216	92-93	-0.009554753595356402
2216	94-95	-0.0209796299156757
2216	96-97	0.038470228909176285
2216	98-99	0.09750841450085446
2216	100	0.41957743493606614
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	530.0
6	3784.0
7	9600.0
8	13528.0
9	18973.0
10	28325.0
11	38887.0
12	46328.0
13	53219.0
14	60887.0
15	70103.0
16	81372.0
17	96128.0
18	114787.0
19	131114.0
20	149462.0
21	169316.0
22	189351.0
23	220599.0
24	306235.0
25	435572.0
26	337337.0
27	767510.0
28	462298.0
29	839038.0
30	664786.0
31	809975.0
32	1089765.0
33	2035875.0
34	2416433.0
35	6800325.0
36	2328004.0
37	1115270.0
38	204843.0
39	6933.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.190618496290611	36.4013634845425	25.876138486891758	23.53187953227513
2	11.49164026283633	41.463360375829275	26.014434973102567	21.030564388231824
3	9.840898919567133	45.02114845807923	23.40100647000932	21.736946152344316
4	8.526790671798762	45.31184494619945	22.97392933349683	23.187435048504952
5	8.408263421367888	44.161349182431856	24.1994112541742	23.23097614202606
6	8.493986407038951	41.169906406185255	25.45134729550646	24.884759891269333
7	11.064908642776457	46.53095575175064	21.010300806780556	21.393834798692357
8	10.240903332560759	43.43097865458495	18.662314924427935	27.665803088426355
9	8.393274235993696	42.47393779107785	19.34206515260593	29.790722820322525
10-11	10.192660036291096	57.428595722065545	18.526386553679547	13.852357687963812
12-13	9.820526328235527	58.118946290921315	18.31502023624621	13.745507144596955
14-15	10.645605479404734	56.93449802470264	18.09928118103398	14.320615314858642
16-17	10.53165499040703	56.69834556460952	18.610195597554448	14.159803847429
18-19	10.773969911197842	55.574601947046965	19.436680370332205	14.214747771422987
20-21	10.164432066952399	55.11854300201085	20.095841161768487	14.621183769268258
22-23	10.319203001923	55.619976316314215	20.57954268511866	13.481277996644133
24-25	10.343652383428015	55.56501555896759	20.200769958871444	13.890562098732955
26-27	11.417149254950127	55.69091700108507	19.511678291317804	13.380255452646997
28-29	11.07136452747102	56.346752963601034	19.979197889576227	12.602684619351717
30-31	11.342291398662995	56.26412042399015	19.825817941815906	12.567770235530945
32-33	11.688656174693508	56.36850134635939	19.38169269998914	12.561149778957963
34-35	11.701783928125252	56.52786244317245	19.28053721402426	12.489816414678035
36-37	12.046323123755212	56.42881173105808	19.023849216808802	12.501015928377903
38-39	12.346052569734983	56.491680716034764	18.950994482296394	12.211272231933863
40-41	12.500687565601332	56.63174628459671	19.004385566396817	11.86318058340515
42-43	12.618789922287455	56.64609536635259	19.18108265104087	11.55403206031909
44-45	12.775721548746457	56.24589713449102	19.18993154515071	11.788449771611816
46-47	12.740298407314054	55.96168161595513	19.47307474326214	11.824945233468686
48-49	12.698842030538563	56.087320211789496	19.667991481396076	11.545846276275869
50-51	12.966139407372937	56.00136838974474	19.74733486383428	11.28515733904804
52-53	13.143998398841445	56.02059377833788	19.859027782691786	10.97638004012889
54-55	13.141928497960157	56.21559454240391	19.745689669984326	10.89678728965161
56-57	13.025489421964604	56.40342975806089	19.451891982004913	11.119188837969594
58-59	12.810382736723383	56.386874647433636	19.60902188258091	11.193720733262062
60-61	12.821350401742048	56.20618866214885	19.867689236889618	11.104771699219485
62-63	12.842740161059792	55.76653833304896	20.08469082813017	11.306030677761065
64-65	12.723091967557659	55.70809850171597	20.11731303835027	11.4514964923761
66-67	12.456529268933911	55.522980021012856	20.418535306834684	11.601955403218549
68-69	12.340022905537007	55.554472904183946	20.21698835391417	11.888515836364878
70-71	12.22470210828095	55.56552905677368	20.25003771525304	11.95973111969233
72-73	12.325904032999231	55.793738346314505	20.15639394016945	11.723963680516816
74-75	12.296132332388183	55.99261725332705	20.01122920833064	11.700021205954126
76-77	11.923947386435765	56.373477794287375	20.027856218721777	11.674718600555082
78-79	11.609150080299262	56.78868381895451	19.825669090991383	11.77649700975485
80-81	11.281278663847266	57.400195439792434	19.601712747816382	11.716813148543919
82-83	10.916236407233374	57.9531948784894	19.651012894071457	11.479555820205768
84-85	10.548902701913754	58.71540858236658	19.54645356072736	11.189235154992303
86-87	10.246721165024654	59.27860396822664	19.36530330490975	11.109371561838953
88-89	10.032993423018919	59.83982755466507	19.214643583403426	10.912535438912588
90-91	9.942353414478125	60.3431552691344	18.96304195640961	10.751449359977872
92-93	9.949866708288981	60.623017147750225	18.608432592705263	10.818683551255527
94-95	10.004406925952226	60.780834572256104	18.341680976044685	10.87307752574699
96-97	10.137920223313662	60.61229355531586	18.223866285988784	11.025919935381703
98-99	10.331292240947622	60.280856874472256	18.088660350847842	11.299190533732283
100	10.732693303652614	59.421929542717564	18.059138011814472	11.786239141815349
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3273736.0
1	3069017.0
2	1730496.0
3	351642.0
4	99916.5
5	90073.0
6	78195.0
7	59836.5
8	44524.0
9	38564.0
10	41314.5
11	50400.5
12	65812.0
13	87852.5
14	117266.5
15	153789.0
16	194230.0
17	237084.0
18	282229.5
19	326479.0
20	366691.0
21	401596.0
22	431269.5
23	454654.5
24	470515.5
25	477355.0
26	475058.0
27	463332.0
28	442571.0
29	416297.0
30	386089.0
31	353133.0
32	321090.5
33	291909.0
34	264786.0
35	240118.5
36	217508.0
37	197778.0
38	181792.0
39	169823.0
40	162338.5
41	160505.0
42	167345.0
43	185859.0
44	217988.5
45	262443.0
46	313824.5
47	363844.0
48	401767.5
49	425102.0
50	437591.0
51	441757.0
52	441656.0
53	435398.0
54	415892.5
55	377160.5
56	318174.5
57	245878.5
58	174481.0
59	114948.5
60	71523.5
61	43945.0
62	28136.5
63	19602.5
64	14587.0
65	11553.0
66	9556.0
67	8086.5
68	6987.5
69	5977.5
70	5044.0
71	4307.5
72	3713.5
73	3206.0
74	2857.5
75	2587.0
76	2308.0
77	2039.0
78	1835.5
79	1764.0
80	1701.5
81	1629.5
82	1540.5
83	1442.0
84	1359.0
85	1338.5
86	1345.5
87	1267.5
88	1168.5
89	1127.5
90	1075.5
91	1040.0
92	1064.5
93	1039.5
94	960.0
95	925.5
96	903.0
97	826.0
98	755.5
99	661.0
100	549.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014306057204515034
2	3.662422988513647E-4
3	0.0
4	9.043019724725054E-6
5	4.521509862362527E-6
6	0.0042592622903455
7	3.752853185760897E-4
8	0.0
9	0.0
10-11	2.780728565352954E-4
12-13	1.3564529587087582E-5
14-15	4.024143777502649E-4
16-17	2.1929322832458256E-4
18-19	1.7633888463213854E-4
20-21	7.686566766016295E-5
22-23	7.234415779780043E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	2.215539832557638E-4
32-33	5.1997363417169056E-5
34-35	1.4468831559560086E-4
36-37	1.6051360011386972E-4
38-39	6.759657244231978E-4
40-41	0.001964596035196518
42-43	0.0011620280346271693
44-45	4.6797627075452155E-4
46-47	5.629279778641346E-4
48-49	9.314310316466805E-4
50-51	0.0
52-53	9.26909521784318E-5
54-55	4.6119400596097775E-4
56-57	6.126645863501224E-4
58-59	4.6119400596097775E-4
60-61	6.104038314189412E-5
62-63	2.780728565352954E-4
64-65	1.7407812970095729E-4
66-67	2.0120718887513245E-4
68-69	8.816944231606927E-5
70-71	5.538849581394095E-4
72-73	5.06409104584603E-4
74-75	3.4363474953955206E-4
76-77	3.6172078898900217E-4
78-79	7.935249808446235E-4
80-81	0.0010489902880681063
82-83	4.5893325102979647E-4
84-85	8.2291479494998E-4
86-87	0.0013767997530893894
88-89	0.0012660227614615075
90-91	0.0012592404966679637
92-93	7.437883723586357E-4
94-95	8.251755498811613E-4
96-97	7.686566766016296E-4
98-99	3.662422988513647E-4
100	0.0010851623669670065
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.2116506E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.59498486425092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.2649472618104	52.60148196111497
2	9.625228505904913	11.472306942487196
3	1.5932704812365035	2.8485279064184166
4	0.28804509283314156	0.6866417179045129
5	0.07635632032799748	0.2275226877118453
6	0.03152504233687458	0.11272406525465462
7	0.01711298151592747	0.07138935120967324
8	0.015915122285919462	0.07587691773936582
9	0.010478836238881836	0.05620374783460144
>10	0.06666655326179692	0.7131675809957536
>50	0.004966563366880431	0.20530526984176597
>100	0.003417002436847632	0.4028315561715866
>500	5.785110158363939E-4	0.24812881147217594
>1k	7.992075791473752E-4	1.037977286718828
>5k	1.6742189750617825E-4	0.7515782740061528
>10k+	5.250959512693771E-4	28.48833592311842
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1513455	6.8431017087418775	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	441971	1.9983762353782284	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	343417	1.5527633524029518	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	327331	1.4800303447569882	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	239250	1.0817712345702346	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	199588	0.902439110409212	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	195309	0.8830915697081628	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	190484	0.8612752846222635	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	152678	0.6903350827657859	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	149716	0.6769423705534681	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	147388	0.6664162955938882	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	141848	0.6413671309563997	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	131962	0.5966674844570837	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124766	0.564130699487523	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	111348	0.5034610801543427	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	104439	0.47222196851528	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	101321	0.45812390076443354	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	98476	0.44526020520601217	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91211	0.41241143605594843	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72729	0.3288448907797642	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	71269	0.3222434863807149	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70105	0.3169804489009249	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67039	0.30311749966292145	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65068	0.2942056037242049	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54035	0.24431978541275914	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53700	0.24280507960886769	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51365	0.23224735408025118	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48535	0.21945148116976526	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44857	0.20282136789599586	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43180	0.1952387958568139	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41511	0.18769239589653086	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38120	0.17235995595325954	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36022	0.16287382826202296	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35971	0.16264323125904245	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33954	0.15352334586665725	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31145	0.1408224246632809	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30848	0.13947953623415923	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29743	0.13448326783624864	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28051	0.12683287314913125	No Hit
TAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27419	0.12397527891611813	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26526	0.11993757060902838	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25256	0.11419525308382797	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25094	0.11346276848612526	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25019	0.11312365524644805	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24325	0.10998572740196846	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23907	0.10809573627950093	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23509	0.10629617535428064	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0017814748857708355	0.0	0.0	4.521509862362527E-6	0.0
2	0.004575767980710877	0.0	0.0	9.043019724725054E-6	0.0
3	0.008315056636884687	0.0	0.0	3.6172078898900216E-5	0.0
4	0.013166636719199678	0.0	0.0	4.97366084859878E-5	0.0
5	0.020351315890493733	0.0	0.0	6.330113807307538E-5	0.0
6	0.033802807731022254	0.0	0.0	6.78226479354379E-5	0.0
7	0.052937837468540465	0.0	0.0	7.234415779780043E-5	0.0
8	0.11544318980584004	0.0	0.0	7.234415779780043E-5	0.0
9	0.33156231820704407	0.0	0.0	7.686566766016296E-5	0.0
10-11	0.780310868271869	0.0	0.0	7.686566766016296E-5	0.0
12-13	1.297711763331875	0.0	0.0	8.364793245370676E-5	0.0
14-15	1.8395853305219187	0.0	0.0	1.0173397190315686E-4	0.0
16-17	2.379308919772409	0.0	0.0	1.175592564214257E-4	0.0
18-19	2.9383235308506688	0.0	0.0	1.6955661983859474E-4	0.0
20-21	3.655068752722514	0.0	0.0	1.8312114942568234E-4	0.0
22-23	4.48499867022395	0.0	0.0	1.853819043568636E-4	0.0
24-25	5.350194103896881	0.0	0.0	1.8990341421922614E-4	0.0
26-27	6.2552082141727094	0.0	0.0	1.9894643394395117E-4	0.0
28-29	7.27515458363993	0.0	0.0	2.1025020859985748E-4	0.0
30-31	8.455585163406914	0.0	0.0	2.1703247339340128E-4	0.0
32-33	9.781836696990023	0.0	0.0	2.1703247339340128E-4	0.0
34-35	11.003333890081915	0.0	0.0	2.1703247339340128E-4	0.0
36-37	11.940186212053568	0.0	0.0	2.3059700298048888E-4	4.521509862362527E-6
38-39	12.657293154714402	0.0	0.0	2.599868170858453E-4	4.521509862362527E-6
40-41	13.31106278722326	0.0	0.0	2.8711587626002044E-4	4.521509862362527E-6
42-43	13.838727509670832	0.0	0.0	3.368524847460082E-4	4.521509862362527E-6
44-45	14.25862430530392	0.0	0.0	3.4363474953955206E-4	4.521509862362527E-6
46-47	14.616230520318172	0.0	0.0	3.504170143330958E-4	4.521509862362527E-6
48-49	14.950679822572337	0.0	0.0	4.069358876126274E-4	4.521509862362527E-6
50-51	15.290437377404912	0.0	4.521509862362527E-6	4.996268397910592E-4	4.521509862362527E-6
52-53	15.655553820300549	0.0	4.521509862362527E-6	5.49363448277047E-4	4.521509862362527E-6
54-55	16.033032071159884	0.0	4.521509862362527E-6	6.239683610060287E-4	4.521509862362527E-6
56-57	16.436050070476774	0.0	4.521509862362527E-6	6.78226479354379E-4	4.521509862362527E-6
58-59	16.868136856698793	0.0	4.521509862362527E-6	7.121378033220979E-4	4.521509862362527E-6
60-61	17.317082092442632	0.0	4.521509862362527E-6	7.754389413951734E-4	4.521509862362527E-6
62-63	17.737076100537763	0.0	4.521509862362527E-6	8.364793245370676E-4	4.521509862362527E-6
64-65	18.13467280952968	0.0	4.521509862362527E-6	9.223880119219555E-4	4.521509862362527E-6
66-67	18.497166776705143	2.2607549311812635E-6	4.521509862362527E-6	0.0010173397190315686	4.521509862362527E-6
68-69	18.817644613484607	6.78226479354379E-6	4.521509862362527E-6	0.0010761193472422813	4.521509862362527E-6
70-71	19.115329519047897	9.043019724725054E-6	4.521509862362527E-6	0.0010942053866917316	4.521509862362527E-6
72-73	19.400308981897954	9.043019724725054E-6	4.521509862362527E-6	0.0011213344458659066	4.521509862362527E-6
74-75	19.677495622500224	9.043019724725054E-6	4.521509862362527E-6	0.0011642887895583506	4.521509862362527E-6
76-77	19.94992111321743	9.043019724725054E-6	4.521509862362527E-6	0.0012230684177690636	4.521509862362527E-6
78-79	20.216328474307833	9.043019724725054E-6	4.521509862362527E-6	0.0013270631446034016	4.521509862362527E-6
80-81	20.478576046324857	9.043019724725054E-6	4.521509862362527E-6	0.0014898374996484528	4.521509862362527E-6
82-83	20.738829632492582	9.043019724725054E-6	4.521509862362527E-6	0.0015667031673086155	4.521509862362527E-6
84-85	21.002998846201113	9.043019724725054E-6	4.521509862362527E-6	0.0015893107166204282	4.521509862362527E-6
86-87	21.269451422390137	9.043019724725054E-6	4.521509862362527E-6	0.0016006144912763345	4.521509862362527E-6
88	21.471868115153452	9.043019724725054E-6	4.521509862362527E-6	0.0016006144912763345	4.521509862362527E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	5330	0.0	40.474365	9
TGAAAAA	24390	0.0	37.15786	1
CTGCTGA	5700	0.0	34.054127	9
CCTGCTG	4500	0.0	33.839592	8
GCCTGCT	1390	0.0	33.474567	7
GCTTGCT	1340	0.0	32.619156	7
CTTGCTG	3855	0.0	32.064465	8
TACCCGA	10295	0.0	30.77074	6
CCCGACG	11715	0.0	30.169504	8
TGCCAAA	2345	0.0	29.66356	6
GTACGAC	930	0.0	29.312271	6
ACCCGAC	11155	0.0	29.240479	7
TCGCGAC	750	0.0	28.8271	6
GAAAACA	9285	0.0	28.553057	1
CTACCCG	10430	0.0	28.389688	5
ATGCCAA	1480	0.0	28.263939	5
AGTTGCC	525	0.0	27.752735	3
ACTTGCC	1110	0.0	27.522875	3
GACACGC	940	0.0	27.500475	2
TTGCCAA	1275	0.0	26.910208	5
>>END_MODULE