##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248479_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22116506
Sequences flagged as poor quality	0
Sequence length	100
%GC	25
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.443077175029366	34.0	31.0	34.0	31.0	34.0
2	32.619118318237064	34.0	31.0	34.0	31.0	34.0
3	32.75714694717149	34.0	31.0	34.0	31.0	34.0
4	36.237274956541505	37.0	37.0	37.0	35.0	37.0
5	36.16462989226237	37.0	37.0	37.0	35.0	37.0
6	36.15188823225513	37.0	37.0	37.0	35.0	37.0
7	36.14792707311001	37.0	37.0	37.0	35.0	37.0
8	36.128445514856644	37.0	36.0	37.0	35.0	37.0
9	37.90546051894454	39.0	38.0	39.0	35.0	39.0
10-11	37.96986556104296	39.0	38.0	39.0	35.0	39.0
12-13	37.947623214082725	39.0	38.0	39.0	35.0	39.0
14-15	39.4206258438833	41.0	39.0	41.0	36.0	41.0
16-17	39.35264923853705	41.0	39.0	41.0	36.0	41.0
18-19	38.970866713756685	40.0	38.5	41.0	35.5	41.0
20-21	38.453700191160394	40.0	37.0	41.0	35.0	41.0
22-23	37.89035982898926	39.5	35.5	41.0	34.5	41.0
24-25	37.39666527796027	39.0	35.0	41.0	34.0	41.0
26-27	37.191413055932074	38.0	35.0	41.0	33.0	41.0
28-29	37.07840754773832	38.0	35.0	41.0	33.0	41.0
30-31	36.98325892887421	38.0	35.0	41.0	33.0	41.0
32-33	36.982624063674436	38.0	35.0	41.0	33.0	41.0
34-35	36.930893243263654	38.0	35.0	41.0	33.0	41.0
36-37	36.91167689417126	38.0	35.0	41.0	33.0	41.0
38-39	36.82093439623782	38.0	35.0	41.0	33.0	41.0
40-41	36.784674984375926	38.0	35.0	41.0	33.0	41.0
42-43	36.718375135747024	38.0	35.0	41.0	33.0	41.0
44-45	36.590241220742556	37.5	35.0	40.5	33.0	41.0
46-47	36.4354973611112	37.0	35.0	40.0	33.0	41.0
48-49	36.24618992710693	36.5	35.0	40.0	33.0	41.0
50-51	36.02316062039817	35.5	35.0	40.0	33.0	41.0
52-53	35.795348935315545	35.0	35.0	39.5	32.0	41.0
54-55	35.551772192226025	35.0	35.0	39.0	32.0	41.0
56-57	35.2845243728824	35.0	35.0	39.0	31.0	41.0
58-59	35.08004550990107	35.0	35.0	38.5	31.0	41.0
60-61	34.87319423330249	35.0	35.0	37.5	31.0	40.0
62-63	34.65931781900812	35.0	35.0	37.0	31.0	40.0
64-65	34.4599943137492	35.0	34.5	36.5	30.5	40.0
66-67	34.271847686971896	35.0	34.0	36.0	30.0	39.5
68-69	34.101347247164625	35.0	34.0	35.5	30.0	39.0
70-71	33.93898505035108	35.0	34.0	35.0	30.0	39.0
72-73	33.77128007923132	35.0	34.0	35.0	29.5	38.5
74-75	33.6062971022638	35.0	34.0	35.0	29.0	37.0
76-77	33.44895063894812	35.0	34.0	35.0	29.0	37.0
78-79	33.30500909592139	35.0	33.0	35.0	29.0	36.0
80-81	33.16808864383913	35.0	33.0	35.0	29.0	36.0
82-83	33.02702929205906	35.0	33.0	35.0	29.0	36.0
84-85	32.87555602137155	35.0	33.0	35.0	29.0	35.5
86-87	32.753787872279645	35.0	33.0	35.0	28.5	35.0
88-89	32.63518256455156	35.0	33.0	35.0	27.0	35.0
90-91	32.52454085649876	35.0	33.0	35.0	27.0	35.0
92-93	32.373895994240684	35.0	33.0	35.0	27.0	35.0
94-95	32.20414662243665	35.0	33.0	35.0	26.5	35.0
96-97	32.03506094045777	35.0	33.0	35.0	25.0	35.0
98-99	31.703762181060608	35.0	33.0	35.0	24.5	35.0
100	30.164402324670995	34.0	30.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.2546583133045175
1101	2	0.2763711964202713
1101	3	0.2581146707231028
1101	4	0.1594677454592741
1101	5	0.16353915128214425
1101	6	0.16737927994643087
1101	7	0.20140255244562866
1101	8	0.1931764768990476
1101	9	0.25000986484315035
1101	10-11	0.2548858346970633
1101	12-13	0.2680734242312468
1101	14-15	0.3654665327229978
1101	16-17	0.38161628284649396
1101	18-19	0.39406807582656
1101	20-21	0.39696035680076136
1101	22-23	0.37598456310763595
1101	24-25	0.3404704819920994
1101	26-27	0.40490961250579005
1101	28-29	0.3916797845956381
1101	30-31	0.39577542156916223
1101	32-33	0.38496271189172404
1101	34-35	0.3784587868797118
1101	36-37	0.3805716899030003
1101	38-39	0.4073086954717837
1101	40-41	0.37794188509978
1101	42-43	0.3855949118367761
1101	44-45	0.40612202290468247
1101	46-47	0.38518505265483327
1101	48-49	0.35303649647948987
1101	50-51	0.3613106459826625
1101	52-53	0.35019066357055095
1101	54-55	0.3722071775280753
1101	56-57	0.3912912855065329
1101	58-59	0.38248263456302567
1101	60-61	0.3520377473176737
1101	62-63	0.37435738553102027
1101	64-65	0.3233382896663741
1101	66-67	0.35479106275395367
1101	68-69	0.367410126772711
1101	70-71	0.3872362097054136
1101	72-73	0.37037425431400095
1101	74-75	0.4042295629382977
1101	76-77	0.37578804848671155
1101	78-79	0.39506629918949443
1101	80-81	0.3423836240142606
1101	82-83	0.3395098313269287
1101	84-85	0.3471923417148304
1101	86-87	0.38245713150375593
1101	88-89	0.35242630813043974
1101	90-91	0.39142127620174705
1101	92-93	0.37113272776942097
1101	94-95	0.33734270558643686
1101	96-97	0.3425116689412988
1101	98-99	0.4974290316161749
1101	100	0.9368537329663127
1102	1	0.20360232376726373
1102	2	0.22410111056019133
1102	3	0.21625182438857848
1102	4	0.12794961093365487
1102	5	0.11666121966209886
1102	6	0.13010999029247472
1102	7	0.18563716565134314
1102	8	0.1786022949649606
1102	9	0.2149088003589057
1102	10-11	0.22519010117071758
1102	12-13	0.24798219475479755
1102	14-15	0.3172610130999587
1102	16-17	0.3064923594688622
1102	18-19	0.3379395315350351
1102	20-21	0.36088458356549324
1102	22-23	0.3042981441677455
1102	24-25	0.30521421525094894
1102	26-27	0.3122469709884612
1102	28-29	0.30643620150345185
1102	30-31	0.3434955136717548
1102	32-33	0.3322798548834527
1102	34-35	0.32328729456111205
1102	36-37	0.31591216437128367
1102	38-39	0.35349710767368236
1102	40-41	0.30906957343514563
1102	42-43	0.30467129894759637
1102	44-45	0.3188104854959377
1102	46-47	0.3078479485270478
1102	48-49	0.3283690118429945
1102	50-51	0.312455050307328
1102	52-53	0.29196484667579625
1102	54-55	0.30587092686725015
1102	56-57	0.3179002668318205
1102	58-59	0.2979011001801197
1102	60-61	0.3069756492004174
1102	62-63	0.29526254801100293
1102	64-65	0.268350842227683
1102	66-67	0.27127957132554315
1102	68-69	0.2912674558188826
1102	70-71	0.2779789661156187
1102	72-73	0.2798164387957556
1102	74-75	0.29394070143428763
1102	76-77	0.32358590265660325
1102	78-79	0.29583301393181216
1102	80-81	0.29455119636837424
1102	82-83	0.28683028250576115
1102	84-85	0.291947367109735
1102	86-87	0.2743627115091414
1102	88-89	0.31838770578563214
1102	90-91	0.3012011568468438
1102	92-93	0.2778463397927382
1102	94-95	0.2581778942464723
1102	96-97	0.29631307562966924
1102	98-99	0.46387255671124805
1102	100	0.844402494738226
1103	1	0.2006826273624327
1103	2	0.17705088813428205
1103	3	0.1935426909834277
1103	4	0.12018233789235921
1103	5	0.11713931445998327
1103	6	0.1136062408755052
1103	7	0.1561305707925058
1103	8	0.16019530012633254
1103	9	0.18495434941899447
1103	10-11	0.20899899779407605
1103	12-13	0.2347503680064449
1103	14-15	0.3173795349542843
1103	16-17	0.3413662069059882
1103	18-19	0.31685070996157094
1103	20-21	0.3125859601531289
1103	22-23	0.24792533742422052
1103	24-25	0.22883095288913324
1103	26-27	0.2456877301524898
1103	28-29	0.24258120358368274
1103	30-31	0.27455862534489484
1103	32-33	0.24697460888078382
1103	34-35	0.23858820725251206
1103	36-37	0.23468436276142768
1103	38-39	0.25650921547171635
1103	40-41	0.22863877578834035
1103	42-43	0.23466126689071132
1103	44-45	0.25333854661963784
1103	46-47	0.22451790629796164
1103	48-49	0.22008472920273903
1103	50-51	0.2331048117619261
1103	52-53	0.23302169582238008
1103	54-55	0.2572629152809398
1103	56-57	0.26638209755873987
1103	58-59	0.24566822781676478
1103	60-61	0.2625964473200426
1103	62-63	0.2532408575588434
1103	64-65	0.22814972018238677
1103	66-67	0.24561296001546395
1103	68-69	0.2617024536924788
1103	70-71	0.216886480996358
1103	72-73	0.22531051079961628
1103	74-75	0.26364466367650863
1103	76-77	0.22202761171497798
1103	78-79	0.23585746289769816
1103	80-81	0.23927471123766253
1103	82-83	0.2459138435772985
1103	84-85	0.25299911322218094
1103	86-87	0.26942428854052736
1103	88-89	0.28777929675401737
1103	90-91	0.28296955110824484
1103	92-93	0.2957574747396947
1103	94-95	0.2794566714216131
1103	96-97	0.31955176604489566
1103	98-99	0.5104474724435768
1103	100	0.8870135676176503
1104	1	0.17302683776716066
1104	2	0.16898085690035458
1104	3	0.1546446501162606
1104	4	0.09959234688403029
1104	5	0.12152746406484738
1104	6	0.10902217865563557
1104	7	0.13790643362946042
1104	8	0.13476804917787888
1104	9	0.16823357038954612
1104	10-11	0.18069238119651487
1104	12-13	0.18424244689956026
1104	14-15	0.24215347961420974
1104	16-17	0.24901909198854355
1104	18-19	0.23043907513739015
1104	20-21	0.23727660073299006
1104	22-23	0.22721247291083557
1104	24-25	0.1986114168734261
1104	26-27	0.1996223286692711
1104	28-29	0.206630919009811
1104	30-31	0.24114426431098934
1104	32-33	0.24171970952323107
1104	34-35	0.21335406980919913
1104	36-37	0.22497745773371491
1104	38-39	0.22085548190884907
1104	40-41	0.1736431752493175
1104	42-43	0.17279126721996363
1104	44-45	0.17951438925535257
1104	46-47	0.21040749574237338
1104	48-49	0.1977636329257919
1104	50-51	0.18743779863253707
1104	52-53	0.1896344829779366
1104	54-55	0.19473801062139984
1104	56-57	0.22702430315634814
1104	58-59	0.2064488932283055
1104	60-61	0.2211288580701094
1104	62-63	0.21215617901656003
1104	64-65	0.18328469643670076
1104	66-67	0.17973638876193831
1104	68-69	0.20669108870039565
1104	70-71	0.2032872355477764
1104	72-73	0.16794401247992852
1104	74-75	0.22172519946095548
1104	76-77	0.24030412535385892
1104	78-79	0.22593718817109476
1104	80-81	0.22304008242571172
1104	82-83	0.2246297239952142
1104	84-85	0.2119627415279126
1104	86-87	0.18428203332585724
1104	88-89	0.15294082102433748
1104	90-91	0.17977809526125554
1104	92-93	0.18079173665949355
1104	94-95	0.16252667914677943
1104	96-97	0.1819718124335168
1104	98-99	0.33360232962077063
1104	100	0.7122886178288113
1105	1	0.1738666732737144
1105	2	0.15767670210852458
1105	3	0.1446857437296103
1105	4	0.08419105369050328
1105	5	0.10159504446704659
1105	6	0.09672839153865453
1105	7	0.1354078111153072
1105	8	0.12102187231852923
1105	9	0.1455747858017702
1105	10-11	0.1543235759925139
1105	12-13	0.1682469488442564
1105	14-15	0.21635378003163197
1105	16-17	0.2277046343940441
1105	18-19	0.23526470944432987
1105	20-21	0.2349084513426618
1105	22-23	0.190223631805992
1105	24-25	0.18452044628124753
1105	26-27	0.20577622016650565
1105	28-29	0.20145736485802246
1105	30-31	0.20794386466059933
1105	32-33	0.18989049295846883
1105	34-35	0.17763960126717393
1105	36-37	0.1617915848657816
1105	38-39	0.21023065326850343
1105	40-41	0.1821992624565496
1105	42-43	0.17244529801311614
1105	44-45	0.17315731095951037
1105	46-47	0.1973075805226543
1105	48-49	0.1924353759112023
1105	50-51	0.18107927233238996
1105	52-53	0.18219847975666426
1105	54-55	0.17370724357376588
1105	56-57	0.1797001249597372
1105	58-59	0.17041238373801804
1105	60-61	0.15463273306738046
1105	62-63	0.17424094558963787
1105	64-65	0.17503518133894858
1105	66-67	0.17598736836355044
1105	68-69	0.1638346955796237
1105	70-71	0.14559341688277527
1105	72-73	0.16997042237331073
1105	74-75	0.1531775832935196
1105	76-77	0.1621570353020303
1105	78-79	0.1418495544077416
1105	80-81	0.17471668043526734
1105	82-83	0.15137952942855293
1105	84-85	0.15437087164968943
1105	86-87	0.16535538922972393
1105	88-89	0.1609558143307126
1105	90-91	0.18899097246203667
1105	92-93	0.2097388665075215
1105	94-95	0.1972867381556398
1105	96-97	0.25448613131429454
1105	98-99	0.36543137835479556
1105	100	0.7874591241536599
1106	1	0.13902738581415264
1106	2	0.1469346039706707
1106	3	0.14545471557281786
1106	4	0.08604937563110582
1106	5	0.06222752558438316
1106	6	0.08521616203831428
1106	7	0.11928410901410302
1106	8	0.11930221388525553
1106	9	0.11944115084112639
1106	10-11	0.11402169705122844
1106	12-13	0.11956119842659518
1106	14-15	0.13781184260795953
1106	16-17	0.14233087714573145
1106	18-19	0.13313017356989576
1106	20-21	0.13464431044665304
1106	22-23	0.12237664354334754
1106	24-25	0.13584241974264444
1106	26-27	0.1777513208032886
1106	28-29	0.12607413582743732
1106	30-31	0.1807591629680303
1106	32-33	0.15439358740728437
1106	34-35	0.16438414365244114
1106	36-37	0.12807378474387576
1106	38-39	0.13707174661299604
1106	40-41	0.11392380009119307
1106	42-43	0.11580060291963434
1106	44-45	0.11846160481122325
1106	46-47	0.09397594963475342
1106	48-49	0.11214216536684063
1106	50-51	0.0761090267749438
1106	52-53	0.1056728127210107
1106	54-55	0.08535487598629032
1106	56-57	0.11505312051535554
1106	58-59	0.13271146470544437
1106	60-61	0.1351168871129289
1106	62-63	0.09995733503615867
1106	64-65	0.09885969345291556
1106	66-67	0.12652756094722406
1106	68-69	0.10897288156402851
1106	70-71	0.11015923401763672
1106	72-73	0.11228592836400964
1106	74-75	0.10253137324675521
1106	76-77	0.10974981015355922
1106	78-79	0.13748618912792665
1106	80-81	0.12615643307723445
1106	82-83	0.08213284563663592
1106	84-85	0.11429199922523736
1106	86-87	0.08948086486085316
1106	88-89	0.0917591586039137
1106	90-91	0.12498934289325092
1106	92-93	0.09090793176721945
1106	94-95	0.07441064108923001
1106	96-97	0.07369079238720389
1106	98-99	0.2601207706225459
1106	100	0.6377548357503393
1107	1	0.0795498806485
1107	2	0.0938107574642899
1107	3	0.07695068033219599
1107	4	0.058179161302462035
1107	5	0.014291862508294173
1107	6	0.047431961465200345
1107	7	0.0804122336941262
1107	8	0.0845828563814095
1107	9	0.09799629922959241
1107	10-11	0.099818347217699
1107	12-13	0.10901038064734792
1107	14-15	0.12296498044307214
1107	16-17	0.09743659538166582
1107	18-19	0.12310670438954219
1107	20-21	0.14607305280975424
1107	22-23	0.13570316646527658
1107	24-25	0.14751336072399823
1107	26-27	0.16299854257508173
1107	28-29	0.1425799124342717
1107	30-31	0.170741813902751
1107	32-33	0.1536581706899156
1107	34-35	0.15770765089753525
1107	36-37	0.14017314957385452
1107	38-39	0.2030767374997069
1107	40-41	0.18297546322811797
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2216	38-39	-0.48845869049460333
2216	40-41	-0.461984434318353
2216	42-43	-0.42550235549504123
2216	44-45	-0.4197698236286129
2216	46-47	-0.37897835609037145
2216	48-49	-0.3563535840139025
2216	50-51	-0.38165705258264637
2216	52-53	-0.34093372592720783
2216	54-55	-0.31910751312423
2216	56-57	-0.32398693574012327
2216	58-59	-0.3431122829434159
2216	60-61	-0.31351116487434894
2216	62-63	-0.3217556581430685
2216	64-65	-0.33094424441114967
2216	66-67	-0.34938280136542943
2216	68-69	-0.36104995393884565
2216	70-71	-0.35341287245996256
2216	72-73	-0.36237954673337214
2216	74-75	-0.39938156737772346
2216	76-77	-0.3716898117602483
2216	78-79	-0.4326611687395072
2216	80-81	-0.4196541300793655
2216	82-83	-0.3929635933533149
2216	84-85	-0.4403016945560765
2216	86-87	-0.45228550180983973
2216	88-89	-0.4492762317774819
2216	90-91	-0.45126641604765894
2216	92-93	-0.46168471857940574
2216	94-95	-0.4600746308707784
2216	96-97	-0.49689095642079906
2216	98-99	-0.3037638485277405
2216	100	0.24026537186355412
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	15.0
7	66.0
8	225.0
9	597.0
10	1314.0
11	2637.0
12	4260.0
13	6399.0
14	9156.0
15	13168.0
16	18973.0
17	26793.0
18	36325.0
19	47064.0
20	58768.0
21	72890.0
22	88637.0
23	103924.0
24	124281.0
25	147242.0
26	174342.0
27	207945.0
28	252882.0
29	317085.0
30	367775.0
31	462349.0
32	609843.0
33	901235.0
34	1327114.0
35	6510892.0
36	2758433.0
37	4434323.0
38	2552185.0
39	476570.0
40	799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.49694992486174	21.952559110044646	35.514112561316935	24.036378403776677
2	15.070404090002471	21.43410288263999	43.88377129060939	19.611721736748144
3	11.63868289141151	17.196708196131887	51.07568076078564	20.088928151670974
4	11.467341747060347	15.126984284160628	55.18850041080232	18.217173557976707
5	10.180699918341698	15.785273724025158	56.91496721613495	17.119059141498187
6	9.908974387553167	12.559339734798428	52.99201490607537	24.53967097157304
7	13.291177186848591	13.528194733833635	50.400546994177105	22.78008108514066
8	10.497489974230106	12.83127633270825	52.10578922366851	24.56544446939313
9	11.146950607840136	12.679321950763834	53.60135999782244	22.57236744357359
10-11	13.154530376543203	20.282760757960595	54.542496450388676	12.02021241510752
12-13	11.773797660923925	23.95479053195005	52.3425746259099	11.928837181216123
14-15	12.740662712242532	22.113440089461513	52.32254896224626	12.823348236049695
16-17	15.570876391675647	22.648599887215447	52.84108193591537	8.939441785193537
18-19	15.545983216877316	19.733898721063692	52.52608640135892	12.194031660700082
20-21	12.757847648191253	19.17952706866483	55.357889797902494	12.704735485241429
22-23	12.730643452841168	19.689609418330832	56.49988839709861	11.07985873172939
24-25	12.708051649330136	20.451334217087748	55.36780879886154	11.472805334720572
26-27	14.85585826636539	20.173921654205664	53.48545854317865	11.484761536250295
28-29	13.608763628453197	21.96142517482208	54.83344894464679	9.59636225207794
30-31	13.688500784668491	20.875337978086076	54.87026665073229	10.565894586513142
32-33	14.00598515824003	21.289623311143675	54.75660826744577	9.947783263170527
34-35	13.930530442282912	21.406849395808518	54.8568355243969	9.805784637511675
36-37	14.078848320413764	20.84365551440047	54.73069620258888	10.346799962596883
38-39	14.419139554982177	20.943471766788445	54.20882604414593	10.428562634083443
40-41	14.478408088694941	21.398565415095675	54.202505854975655	9.920520641233724
42-43	14.49573371350937	21.43931444236811	54.237245600559504	9.827706243563018
44-45	14.67247869482448	20.72855699066908	54.26968744541417	10.329276869092267
46-47	14.756266176449673	20.541636446961753	54.51914079951498	10.182956577073593
48-49	14.268143754854517	20.517163242782757	54.76925254317051	10.445440459192215
50-51	14.129514345734389	20.45022577691827	54.86292068843319	10.557339188914153
52-53	14.003317340921958	20.60048546055495	54.76802759063997	10.628169607883123
54-55	14.012366342840519	20.633638914970152	54.77734132875528	10.576653413434052
56-57	13.799564354677079	20.57848529597556	54.757755715764844	10.864194633582509
58-59	13.598318224681186	20.68148887351032	55.064210547020096	10.655982354788396
60-61	13.388075584735043	20.433286216196997	55.14853203345358	11.030106165614379
62-63	13.217281724411874	20.21806305015679	55.247413186832276	11.317242038599066
64-65	13.246056647674134	20.137925648672855	55.13665452580786	11.479363177845146
66-67	13.012890374264263	19.938772242786655	55.5557266400729	11.492610742876181
68-69	12.863964620911005	19.587169047945704	55.874826006296566	11.67404032484673
70-71	12.720366545592668	19.50078172427008	56.1489447570211	11.629906973116146
72-73	12.550604843495828	19.209092378666735	56.4948423540796	11.74546042375784
74-75	12.398390930872282	18.828714641997312	56.80319777258126	11.969696654549152
76-77	12.532946724073874	18.730401127453593	56.90792759233233	11.828724556140205
78-79	12.589470483600667	18.84077097679268	57.05119466981553	11.518563869791123
80-81	12.439412945348284	18.819659201760032	57.3343486586686	11.40657919422309
82-83	12.521961353083208	18.92923084046959	57.27304732722603	11.275760479221171
84-85	12.58637216848974	19.185727782648755	56.958197490425476	11.26970255843603
86-87	12.664227518348214	19.454851616944886	56.74384778028746	11.137073084419441
88-89	12.679556141058445	19.91317120582891	56.4716043986515	10.935668254461147
90-91	12.706162511223319	20.21619988162456	56.262038790960766	10.815598816191349
92-93	12.599680090381046	20.277169538306108	56.10122485509535	11.021925516217486
94-95	12.564053741839999	20.519283404754358	55.93895659411704	10.977706259288606
96-97	12.3483779239953	20.931315890553133	56.04738491350155	10.67292127195001
98-99	12.189027308802329	21.31883283642597	55.94572193135809	10.546417923413621
100	12.088491071451994	21.770919545567534	55.58296295679243	10.557626426188046
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	971565.0
1	2035934.0
2	2635302.5
3	1390799.5
4	372016.0
5	98156.0
6	52513.5
7	37902.0
8	33834.0
9	35836.0
10	42562.0
11	54494.0
12	73307.0
13	98823.5
14	131010.5
15	168372.5
16	210103.0
17	256420.5
18	304197.0
19	346047.5
20	378797.5
21	406643.0
22	430751.5
23	449078.0
24	461614.5
25	468504.0
26	466160.5
27	453440.0
28	433172.5
29	406776.0
30	376501.5
31	344927.0
32	313694.0
33	283671.0
34	255550.0
35	228988.0
36	203882.5
37	181333.5
38	160572.0
39	141644.0
40	125616.5
41	112278.5
42	101373.0
43	93867.5
44	93446.5
45	104525.0
46	128760.0
47	165528.0
48	213359.0
49	275368.5
50	350982.0
51	441270.5
52	544307.5
53	637637.0
54	690454.0
55	673783.5
56	581664.5
57	440626.0
58	291225.0
59	168365.0
60	86884.0
61	41626.5
62	19509.0
63	9297.0
64	4736.0
65	2768.5
66	1933.0
67	1453.0
68	1148.5
69	918.5
70	692.0
71	519.0
72	370.5
73	284.0
74	218.5
75	165.0
76	117.0
77	82.0
78	51.5
79	37.5
80	27.5
81	17.5
82	15.5
83	15.0
84	14.5
85	12.0
86	7.5
87	6.0
88	5.5
89	5.5
90	4.5
91	1.5
92	1.5
93	1.0
94	0.5
95	1.0
96	1.0
97	1.0
98	2.0
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017796662818258906
2	0.1173648314973441
3	0.0
4	1.311237860085133E-4
5	0.038554914596365265
6	0.0019125986717793489
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.472563161649494E-4
14-15	8.138717752252547E-4
16-17	9.043019724725054E-6
18-19	9.043019724725054E-6
20-21	4.6571551582334027E-4
22-23	6.578796849737476E-4
24-25	0.0028643764978066606
26-27	0.0023217953143231575
28-29	0.0033233097488364572
30-31	0.0036036433603029343
32-33	0.0036172078898900214
34-35	0.003963103394360755
36-37	0.003174099923378494
38-39	0.0017204345026289416
40-41	0.003169578413516131
42-43	0.005140956713506193
44-45	0.004996268397910592
46-47	0.01248614948491412
48-49	0.005192954076923362
50-51	0.006940517638726478
52-53	0.002712905917417516
54-55	0.0012049823783196133
56-57	0.005733274505475684
58-59	0.0026722123286562535
60-61	0.014371619097519292
62-63	0.0127054427132387
64-65	0.00994732169719756
66-67	0.01211538567620039
68-69	0.00994732169719756
70-71	0.008731035544222039
72-73	0.005792054133686397
74-75	0.0038975415013564984
76-77	5.380596736211408E-4
78-79	0.01039721192850263
80-81	0.006452194573591326
82-83	0.01073632516817982
84-85	0.008864420085161733
86-87	0.007609701098356132
88-89	0.009870456029537397
90-91	0.005771707339305765
92-93	0.009653423556143995
94-95	0.003174099923378494
96-97	0.005794314888617579
98-99	0.45743437051042324
100	1.7561227799725692
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.2116506E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.536114690566585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.23424115876365	49.615831895646025
2	10.911890691521323	12.556555886365937
3	2.0922676593112444	3.611428560284851
4	0.4371281738102164	1.0060262697129014
5	0.11996790696469835	0.34512436271540525
6	0.04564649869977351	0.15757933106477803
7	0.024819225652544765	0.09996012695731074
8	0.016688321794157208	0.07681449573933687
9	0.013642116983599134	0.07064229666514374
>10	0.08077909181713869	0.8792545153180479
>50	0.008886048862440338	0.36084025700806693
>100	0.010426071401939269	1.2805162805604478
>500	0.001516956574936403	0.593182938443872
>1k	9.474974156381847E-4	1.2080889542327506
>5k	2.920922999506955E-4	1.2162170931836713
>10k+	8.604881268817784E-4	26.921936736101422
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	214890	0.9716272543230833	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	174249	0.7878685720068079	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	173832	0.7859831023942029	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	172537	0.7801277471224434	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	156936	0.7095876717597255	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153178	0.6925958376969671	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	152326	0.6887435112942343	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	147793	0.668247507088145	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	139481	0.6306647171121876	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	139103	0.6289555863842146	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127510	0.5765377225498458	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127400	0.5760403564649859	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125319	0.5666310944414096	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122722	0.554888733328854	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120370	0.5442541421325773	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118568	0.5361063813606002	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116377	0.5261997532521638	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	113771	0.514416698550847	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	112462	0.5084980421410145	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	108455	0.49038035212252784	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	108172	0.48910076483147924	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100934	0.45637407644769934	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100448	0.45417662265459113	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	98342	0.4446543228844556	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96781	0.43759624598930774	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94620	0.4278252631767423	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93028	0.4206270194758612	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92811	0.4196458518357285	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88852	0.4017451942906352	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88761	0.40133373689316026	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84444	0.38181437881734126	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77126	0.34872596964457225	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72914	0.32968137010430126	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69623	0.31480108114726624	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67484	0.30512957155167275	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61052	0.276047220116957	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57971	0.26211644823101804	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51590	0.23326469379928277	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48498	0.21928418530485785	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47119	0.2130490232046599	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44054	0.19919059547651874	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42431	0.1918521849699044	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42207	0.19083936676073518	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42152	0.19059068371830523	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41461	0.1874663204034127	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41180	0.18619577613208885	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40344	0.1824157938871538	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40204	0.18178278250642302	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37098	0.16773897287392503	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34958	0.15806294176846922	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33882	0.15319779715656714	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33832	0.15297172166344902	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33711	0.15242461897010315	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33571	0.15179160758937238	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32016	0.14476065975339866	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31132	0.1407636450350702	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30969	0.1400266389275051	No Hit
TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30618	0.13843958896581585	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29750	0.13451491840528518	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29460	0.13320368054520004	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29088	0.1315216788764012	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28636	0.12947795641861332	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22580	0.10209569269214586	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22534	0.10188770323847718	No Hit
CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22213	0.1004362985726588	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0018583405534309985	0.0	0.0	0.0	0.0
2	0.004883230651351529	0.0	0.0	0.0	0.0
3	0.008550175149727538	0.0	0.0	1.356452958708758E-5	0.0
4	0.013596180156124118	0.0	0.0	1.356452958708758E-5	0.0
5	0.021807242066174466	0.0	0.0	1.356452958708758E-5	0.0
6	0.03886237726700592	0.0	0.0	1.356452958708758E-5	0.0
7	0.0645762038542616	0.0	0.0	1.356452958708758E-5	0.0
8	0.16930793679616482	0.0	0.0	1.8086039449450108E-5	0.0
9	0.5549158623880281	0.0	0.0	1.8086039449450108E-5	0.0
10-11	1.281371026689297	4.521509862362527E-6	0.0	2.2607549311812632E-5	0.0
12-13	1.9562041128919732	4.521509862362527E-6	0.0	2.712905917417516E-5	0.0
14-15	2.5844611260024526	4.521509862362527E-6	0.0	3.6172078898900216E-5	0.0
16-17	3.1286474454870943	4.521509862362527E-6	0.0	4.973660848598779E-5	0.0
18-19	3.6609037611998927	4.521509862362527E-6	0.0	7.234415779780043E-5	0.0
20-21	4.343222206979711	4.521509862362527E-6	0.0	8.364793245370676E-5	0.0
22-23	5.174994639750058	4.521509862362527E-6	0.0	8.590868738488801E-5	0.0
24-25	6.136932298438099	4.521509862362527E-6	0.0	8.590868738488801E-5	0.0
26-27	7.199082440960611	4.521509862362527E-6	0.0	8.816944231606927E-5	0.0
28-29	8.413342957517791	4.521509862362527E-6	0.0	9.721246204079433E-5	0.0
30-31	9.767394994489635	4.521509862362527E-6	0.0	9.94732169719756E-5	0.0
32-33	11.311000028666372	4.521509862362527E-6	0.0	9.94732169719756E-5	0.0
34-35	12.815256623265899	4.521509862362527E-6	0.0	9.94732169719756E-5	0.0
36-37	13.8671248523614	4.521509862362527E-6	0.0	1.0173397190315686E-4	0.0
38-39	14.631911116520847	4.521509862362527E-6	0.0	1.28863031077332E-4	0.0
40-41	15.39875918917753	4.521509862362527E-6	0.0	1.4468831559560084E-4	0.0
42-43	16.00450812619317	4.521509862362527E-6	0.0	1.7633888463213854E-4	0.0
44-45	16.464840784525368	4.521509862362527E-6	0.0	1.8086039449450108E-4	0.0
46-47	16.84250215653413	4.521509862362527E-6	0.0	2.0120718887513245E-4	0.0
48-49	17.183686247728282	4.521509862362527E-6	0.0	2.3737926777403268E-4	0.0
50-51	17.519955457702043	4.521509862362527E-6	0.0	2.8033361146647664E-4	0.0
52-53	17.879591830644497	4.521509862362527E-6	0.0	3.278094650212832E-4	0.0
54-55	18.215797739480188	4.521509862362527E-6	0.0	3.707638087137272E-4	0.0
56-57	18.548922239344677	4.521509862362527E-6	0.0	3.798068284384523E-4	0.0
58-59	18.868764803988476	4.521509862362527E-6	0.0	3.888498481631773E-4	4.521509862362527E-6
60-61	19.180586210136447	4.521509862362527E-6	0.0	4.069358876126274E-4	4.521509862362527E-6
62-63	19.485826558679747	4.521509862362527E-6	0.0	4.3406494678680256E-4	4.521509862362527E-6
64-65	19.7858287380475	9.043019724725054E-6	0.0	4.6119400596097775E-4	4.521509862362527E-6
66-67	20.06937940378105	9.043019724725054E-6	0.0	5.357989186899595E-4	4.521509862362527E-6
68-69	20.330634504383287	9.043019724725054E-6	0.0	5.787532623824034E-4	4.521509862362527E-6
70-71	20.588200052937836	9.043019724725054E-6	0.0	6.149253412813037E-4	4.521509862362527E-6
72-73	20.84209413548415	9.043019724725054E-6	0.0	6.465759103178413E-4	4.521509862362527E-6
74-75	21.09294072038323	9.043019724725054E-6	0.0	6.646619497672915E-4	4.521509862362527E-6
76-77	21.343913907558452	9.043019724725054E-6	0.0	7.234415779780042E-4	4.521509862362527E-6
78-79	21.583594623852427	9.043019724725054E-6	0.0	8.74912158367149E-4	4.521509862362527E-6
80-81	21.821272311277376	9.043019724725054E-6	0.0	0.0010444687782057438	4.521509862362527E-6
82-83	22.059479015356224	9.043019724725054E-6	0.0	0.0010851623669670065	4.521509862362527E-6
84-85	22.299293116191137	9.043019724725054E-6	0.0	0.0010942053866917316	4.521509862362527E-6
86-87	22.542136628633834	9.043019724725054E-6	4.521509862362527E-6	0.0010964661416229128	4.521509862362527E-6
88	22.7259088754797	9.043019724725054E-6	9.043019724725054E-6	0.0011122914261411816	4.521509862362527E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	5025	0.0	64.0433	1
TGCCCTA	7860	0.0	54.623516	6
GACTTTT	4785	0.0	49.261703	1
CCCCTAC	50080	0.0	48.80322	7
CCCCCTA	27720	0.0	47.533043	6
GCTTTTC	5020	0.0	46.95563	1
CACTTTT	7550	0.0	46.800083	1
GCCCTAC	21540	0.0	46.537994	7
CGCCCTA	8580	0.0	46.15261	6
TGCTCTA	7905	0.0	45.874508	6
CATTTTT	37095	0.0	45.571728	1
GCTTTCT	3635	0.0	44.65482	1
CCTTTTC	10225	0.0	44.40354	1
GCCCCTA	5785	0.0	44.091255	6
ATGCCCT	2800	0.0	42.963234	5
CCGCCTA	4540	0.0	42.110905	6
TTGCCCT	3610	0.0	42.044613	5
ACTTTTC	10515	0.0	41.74707	1
GCATTTT	4095	0.0	41.476963	1
CTGCCCT	4050	0.0	40.72557	5
>>END_MODULE