##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248478_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15905437
Sequences flagged as poor quality	0
Sequence length	100
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.967913299081314	33.0	31.0	34.0	22.0	34.0
2	30.410583248985866	33.0	31.0	34.0	25.0	34.0
3	30.837959497749104	34.0	31.0	34.0	26.0	34.0
4	34.341326428189305	37.0	35.0	37.0	30.0	37.0
5	34.25505435657002	37.0	35.0	37.0	30.0	37.0
6	34.12244976356198	37.0	35.0	37.0	29.0	37.0
7	34.31075832748261	37.0	35.0	37.0	30.0	37.0
8	34.251589000666876	37.0	35.0	37.0	30.0	37.0
9	35.71872008295025	39.0	35.0	39.0	29.0	39.0
10-11	36.23564501874422	39.0	37.0	39.0	31.0	39.0
12-13	36.29339237897079	39.0	37.0	39.0	31.0	39.0
14-15	37.56059506569986	40.0	38.0	41.0	31.0	41.0
16-17	37.375735668249796	40.0	38.0	41.0	31.0	41.0
18-19	36.716550573241086	39.0	36.0	41.0	30.0	41.0
20-21	36.300759953970456	39.0	35.0	41.0	30.0	41.0
22-23	36.0239822709681	38.0	35.0	41.0	29.0	41.0
24-25	35.81448394030293	38.0	35.0	41.0	27.0	41.0
26-27	35.658792776331765	38.0	35.0	41.0	26.0	41.0
28-29	35.517017168406	38.0	35.0	40.0	25.0	41.0
30-31	35.45827948644228	38.0	35.0	40.0	25.0	41.0
32-33	35.38172764445265	38.0	35.0	40.0	25.0	41.0
34-35	35.277499668823936	38.0	35.0	40.0	25.0	41.0
36-37	35.18040469431931	38.0	34.5	40.0	25.0	41.0
38-39	35.036373725538	38.0	34.0	40.0	25.0	41.0
40-41	34.87667207760466	37.0	34.0	40.0	25.0	41.0
42-43	34.67475986356112	37.0	33.0	40.0	25.0	41.0
44-45	34.517113739157246	36.0	33.0	40.0	25.0	41.0
46-47	34.40231277518499	36.0	33.0	40.0	25.0	41.0
48-49	34.356982804056244	36.0	33.0	39.5	25.0	41.0
50-51	34.16277751438078	35.0	33.0	39.0	25.0	41.0
52-53	33.88251350780239	35.0	33.0	39.0	25.0	41.0
54-55	33.76536202683397	35.0	33.0	39.0	25.0	41.0
56-57	33.67275793805602	35.0	33.0	38.0	25.0	40.0
58-59	33.43270989662214	35.0	33.0	37.0	25.0	40.0
60-61	33.204330129376515	35.0	33.0	36.5	25.0	40.0
62-63	32.95428387160944	35.0	32.0	36.0	25.0	39.0
64-65	32.76927351948896	35.0	32.0	35.5	25.0	39.0
66-67	32.6002868704582	35.0	32.0	35.0	25.0	39.0
68-69	32.42341612493891	35.0	32.0	35.0	25.0	38.0
70-71	32.26859799576711	35.0	31.0	35.0	25.0	37.0
72-73	32.13313504055249	35.0	31.0	35.0	25.0	37.0
74-75	31.991239096417154	35.0	31.0	35.0	24.5	36.0
76-77	31.83051820581855	35.0	31.0	35.0	24.0	36.0
78-79	31.69692046185213	35.0	31.0	35.0	24.0	36.0
80-81	31.560932874714474	35.0	31.0	35.0	24.0	36.0
82-83	31.445151868508862	35.0	31.0	35.0	23.5	35.5
84-85	31.331408530303193	35.0	31.0	35.0	23.0	35.0
86-87	31.16833407343665	35.0	30.5	35.0	22.0	35.0
88-89	31.035654191708154	35.0	30.0	35.0	20.0	35.0
90-91	30.844890146683802	35.0	30.0	35.0	20.0	35.0
92-93	30.607390699167837	35.0	30.0	35.0	18.5	35.0
94-95	30.311564246867285	35.0	29.0	35.0	16.0	35.0
96-97	29.98272122293779	35.0	29.0	35.0	13.5	35.0
98-99	29.568567371019107	34.0	28.0	35.0	10.0	35.0
100	28.413916700308203	33.0	25.0	35.0	8.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5739102389139248
1101	2	0.4773830680826343
1101	3	0.3965812203098835
1101	4	0.3210258782788955
1101	5	0.28534719172600376
1101	6	0.2628323363767251
1101	7	0.30759677803019514
1101	8	0.2580150542697055
1101	9	0.3471220294321
1101	10-11	0.33225127572914204
1101	12-13	0.35297789673484203
1101	14-15	0.4833415928146394
1101	16-17	0.4832577158575049
1101	18-19	0.5016722222120364
1101	20-21	0.5314165775595754
1101	22-23	0.5351070497017716
1101	24-25	0.5211156612097909
1101	26-27	0.5586021670520722
1101	28-29	0.5495993609817518
1101	30-31	0.5339683686467183
1101	32-33	0.5510561842864448
1101	34-35	0.5692333221455428
1101	36-37	0.5143087819828978
1101	38-39	0.5084509095318737
1101	40-41	0.5225288407862578
1101	42-43	0.5276742421951113
1101	44-45	0.5137053819626658
1101	46-47	0.554749597917656
1101	48-49	0.5228088047042831
1101	50-51	0.5006802382006015
1101	52-53	0.4726141730978384
1101	54-55	0.4405637698805691
1101	56-57	0.46227933984525293
1101	58-59	0.5277847701877008
1101	60-61	0.4610771723023106
1101	62-63	0.4775417718598902
1101	64-65	0.46303665294455243
1101	66-67	0.4544701575658294
1101	68-69	0.5125942795472724
1101	70-71	0.5003690981025102
1101	72-73	0.4757722728876743
1101	74-75	0.46095992672506014
1101	76-77	0.4552299056480109
1101	78-79	0.4919533631022297
1101	80-81	0.45089441888269377
1101	82-83	0.46197335508782444
1101	84-85	0.42137136235190553
1101	86-87	0.4251551789774233
1101	88-89	0.39730237619662745
1101	90-91	0.38480019872330473
1101	92-93	0.37042486136310515
1101	94-95	0.3759916073857532
1101	96-97	0.3688210623483137
1101	98-99	0.30798065745846515
1101	100	0.34963330713823737
1102	1	0.2904496642425691
1102	2	0.2114489985532515
1102	3	0.2642445858341951
1102	4	0.259090784067773
1102	5	0.24335227840099094
1102	6	0.2602092439132946
1102	7	0.24564321921917553
1102	8	0.23035136045481153
1102	9	0.2012421217052065
1102	10-11	0.3204299701093589
1102	12-13	0.37175244943153984
1102	14-15	0.49003696377808126
1102	16-17	0.4988648543458254
1102	18-19	0.4860525855049289
1102	20-21	0.470295840849829
1102	22-23	0.46031387014939895
1102	24-25	0.4590740773345203
1102	26-27	0.44969555076661294
1102	28-29	0.5057996604191572
1102	30-31	0.4887663300068894
1102	32-33	0.5029680076798329
1102	34-35	0.532840820906479
1102	36-37	0.5155883962080452
1102	38-39	0.5255513210523048
1102	40-41	0.5544781100624547
1102	42-43	0.536594356603409
1102	44-45	0.5463139933629151
1102	46-47	0.5784081536359196
1102	48-49	0.5448511552734914
1102	50-51	0.4966040782950927
1102	52-53	0.45646406833141384
1102	54-55	0.49854065772575495
1102	56-57	0.4983153858810212
1102	58-59	0.5236930951316268
1102	60-61	0.4623812093640325
1102	62-63	0.4983939504000716
1102	64-65	0.47622230168414603
1102	66-67	0.41417023982719314
1102	68-69	0.4326767837543315
1102	70-71	0.4385238544727912
1102	72-73	0.44828956356602134
1102	74-75	0.4631036574650622
1102	76-77	0.4399599805512011
1102	78-79	0.4789248368218928
1102	80-81	0.44985112593941423
1102	82-83	0.4739390999530322
1102	84-85	0.4743595062381303
1102	86-87	0.4421607520311426
1102	88-89	0.42696388736317203
1102	90-91	0.3735079800991947
1102	92-93	0.3554560558444493
1102	94-95	0.370174198478356
1102	96-97	0.37621344345229346
1102	98-99	0.40680095405476635
1102	100	0.3579323966668504
1103	1	0.3592100292754381
1103	2	0.29171675992992974
1103	3	0.32508463251763686
1103	4	0.2811421459489196
1103	5	0.22618695495143015
1103	6	0.24351201936293876
1103	7	0.2926829076366815
1103	8	0.24490815881205918
1103	9	0.2808127577836572
1103	10-11	0.38009242468449855
1103	12-13	0.3802042934478891
1103	14-15	0.5283118072722175
1103	16-17	0.5419207231894276
1103	18-19	0.5376769425555565
1103	20-21	0.49277219538522843
1103	22-23	0.46523837892748077
1103	24-25	0.49813865489923614
1103	26-27	0.5096683811147287
1103	28-29	0.5001368349015465
1103	30-31	0.5063102110193327
1103	32-33	0.5118209666607569
1103	34-35	0.5068949712182231
1103	36-37	0.506211783653292
1103	38-39	0.5228420172852353
1103	40-41	0.5100056549231979
1103	42-43	0.5066202608083188
1103	44-45	0.572891690751888
1103	46-47	0.5521423111395691
1103	48-49	0.5181168377557981
1103	50-51	0.5066737493963274
1103	52-53	0.5128170120247972
1103	54-55	0.49635595721335335
1103	56-57	0.469128562266107
1103	58-59	0.4891926891976581
1103	60-61	0.4627044449847588
1103	62-63	0.43036990788726115
1103	64-65	0.42552528536207035
1103	66-67	0.4322344334340684
1103	68-69	0.421066119270435
1103	70-71	0.4263160845031493
1103	72-73	0.44533929676107675
1103	74-75	0.4554011612661455
1103	76-77	0.43644164025747045
1103	78-79	0.4194432164794044
1103	80-81	0.40960561308100907
1103	82-83	0.3775185337335891
1103	84-85	0.3696819802170275
1103	86-87	0.3940534690331283
1103	88-89	0.3632622471520719
1103	90-91	0.34636454757612256
1103	92-93	0.31702757031479933
1103	94-95	0.3263650485282561
1103	96-97	0.3378590407491302
1103	98-99	0.31547986510540227
1103	100	0.24205063536834004
1104	1	0.15988894617754212
1104	2	0.17756418119446948
1104	3	0.16858993517233856
1104	4	0.167386580747646
1104	5	0.09402208591546213
1104	6	0.07670333838630938
1104	7	0.08554910205986488
1104	8	0.14538109996207993
1104	9	0.16662689211806025
1104	10-11	0.22862245230351874
1104	12-13	0.24186665911565086
1104	14-15	0.315788328076124
1104	16-17	0.34361005495122754
1104	18-19	0.3279603685451633
1104	20-21	0.28673708699130174
1104	22-23	0.28451724994663863
1104	24-25	0.2908197489295077
1104	26-27	0.28761871620439905
1104	28-29	0.24753832558365474
1104	30-31	0.2906915549575686
1104	32-33	0.28318086901876427
1104	34-35	0.2895962660190108
1104	36-37	0.29027329763551535
1104	38-39	0.3324142081505812
1104	40-41	0.31130230719265484
1104	42-43	0.2936563149662774
1104	44-45	0.34198344695849414
1104	46-47	0.3331582136902611
1104	48-49	0.295637574388401
1104	50-51	0.2671464890216839
1104	52-53	0.2719808110486923
1104	54-55	0.3148427924247841
1104	56-57	0.29697474532632384
1104	58-59	0.29802574293153583
1104	60-61	0.24851498057564214
1104	62-63	0.2818680511266649
1104	64-65	0.28264370798522265
1104	66-67	0.28949254826365944
1104	68-69	0.30896396725241004
1104	70-71	0.2725961964478145
1104	72-73	0.2934826424489927
1104	74-75	0.2937692864925552
1104	76-77	0.25725212051487745
1104	78-79	0.29394130300406474
1104	80-81	0.2905218934617224
1104	82-83	0.2677711425352278
1104	84-85	0.24350014221621663
1104	86-87	0.29409596187342046
1104	88-89	0.2723737701046858
1104	90-91	0.2341388381457854
1104	92-93	0.18040536256364703
1104	94-95	0.2215466518704794
1104	96-97	0.221602211293785
1104	98-99	0.1900598486855003
1104	100	0.13900157200482255
1105	1	0.16599218606302557
1105	2	0.13772510693981488
1105	3	0.13528550381998627
1105	4	0.10771123017527628
1105	5	0.12634265560154034
1105	6	0.07150386888015703
1105	7	0.12030010824892656
1105	8	0.10095511752293618
1105	9	0.09185028971226927
1105	10-11	0.17197635225210917
1105	12-13	0.18425510174004955
1105	14-15	0.24310773697217058
1105	16-17	0.2297473378362369
1105	18-19	0.2521890970165259
1105	20-21	0.244064246411142
1105	22-23	0.2778356254395007
1105	24-25	0.2813330323569758
1105	26-27	0.30258288691546653
1105	28-29	0.27047915863898453
1105	30-31	0.23880994725292481
1105	32-33	0.2668370010834167
1105	34-35	0.30390233905314545
1105	36-37	0.23837724675911431
1105	38-39	0.2885766838052177
1105	40-41	0.30297586754669226
1105	42-43	0.28990303522377303
1105	44-45	0.2875432692017341
1105	46-47	0.32208875790619373
1105	48-49	0.28096628399118373
1105	50-51	0.22438164823267925
1105	52-53	0.2320873496224678
1105	54-55	0.22598201973327292
1105	56-57	0.22401124475966583
1105	58-59	0.22314585884908666
1105	60-61	0.21742239519258533
1105	62-63	0.2112124888724054
1105	64-65	0.18802038928603793
1105	66-67	0.15764373412351773
1105	68-69	0.17938995754938958
1105	70-71	0.19283835833944352
1105	72-73	0.16479494485104595
1105	74-75	0.1961922287310287
1105	76-77	0.17512635283899414
1105	78-79	0.1902358182560171
1105	80-81	0.1808346124659188
1105	82-83	0.15942097887037576
1105	84-85	0.1783904969629191
1105	86-87	0.19708707452403829
1105	88-89	0.20390973193108408
1105	90-91	0.19107070736488652
1105	92-93	0.15720216476840676
1105	94-95	0.193779056049884
1105	96-97	0.19332350624248562
1105	98-99	0.20152225588900308
1105	100	0.12206189342589013
1106	1	0.16749158898032945
1106	2	0.15182563904418345
1106	3	0.19145669721208947
1106	4	0.19824488694411713
1106	5	0.17978050144043323
1106	6	0.1099348748998139
1106	7	0.0784553999643407
1106	8	0.17295495171823205
1106	9	0.15045730541659452
1106	10-11	0.19825240623456608
1106	12-13	0.20609366477997781
1106	14-15	0.24222363537817415
1106	16-17	0.2633552106172701
1106	18-19	0.3174495311490091
1106	20-21	0.28779459099650495
1106	22-23	0.28369587309236266
1106	24-25	0.30353380717683365
1106	26-27	0.29574932850918856
1106	28-29	0.2770161647528724
1106	30-31	0.3066267733264709
1106	32-33	0.28691560988814757
1106	34-35	0.33498577332637325
1106	36-37	0.2952866792058302
1106	38-39	0.3234902038744991
1106	40-41	0.2780106323979581
1106	42-43	0.3080981403334633
1106	44-45	0.2983185623046083
1106	46-47	0.3013655092028742
1106	48-49	0.2801840755412357
1106	50-51	0.30942780268102865
1106	52-53	0.31667867171286446
1106	54-55	0.27813849508709154
1106	56-57	0.28369715068261314
1106	58-59	0.31261672361723924
1106	60-61	0.2590604649578907
1106	62-63	0.25566002886336037
1106	64-65	0.2396722692919937
1106	66-67	0.225141807413209
1106	68-69	0.24200898917825242
1106	70-71	0.25855282733103024
1106	72-73	0.241036486836272
1106	74-75	0.2757885565809701
1106	76-77	0.23576422000206776
1106	78-79	0.22931461570206224
1106	80-81	0.2598317916280486
1106	82-83	0.2519124906210166
1106	84-85	0.19534811560358278
1106	86-87	0.26839359699690846
1106	88-89	0.2100567526639665
1106	90-91	0.20050328543303664
1106	92-93	0.21055548980287142
1106	94-95	0.20619040584333348
1106	96-97	0.1990137215114416
1106	98-99	0.21975235337169607
1106	100	0.22993962082738406
1107	1	0.005930929657516515
1107	2	0.021455602482483016
1107	3	0.08887099597910009
1107	4	0.09166856291533776
1107	5	0.11142870762206769
1107	6	0.04617116367494134
1107	7	0.04093473392498481
1107	8	0.07105137787642235
1107	9	0.06528078517987268
1107	10-11	0.10599758559200012
1107	12-13	0.14200450425154543
1107	14-15	0.17153692865041137
1107	16-17	0.16417538769413653
1107	18-19	0.11487733475301098
1107	20-21	0.07104649277825104
1107	22-23	0.05326560177137907
1107	24-25	0.08749486191901923
1107	26-27	0.10956356998025996
1107	28-29	0.11802951298354003
1107	30-31	0.09491594354343391
1107	32-33	0.06141967159079087
1107	34-35	0.07770724807870977
1107	36-37	0.11349827178745642
1107	38-39	0.12447970728754854
1107	40-41	0.09098346685460257
1107	42-43	0.07355347445606952
1107	44-45	0.11921188268843252
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2216	54-55	-0.4345010888813263
2216	56-57	-0.42240602322766563
2216	58-59	-0.36359482023511447
2216	60-61	-0.3987706076241224
2216	62-63	-0.3331671122911857
2216	64-65	-0.348622118310459
2216	66-67	-0.4136302349085881
2216	68-69	-0.4009664156640298
2216	70-71	-0.4692586846061175
2216	72-73	-0.564137961939359
2216	74-75	-0.5230148441233169
2216	76-77	-0.4481537338969872
2216	78-79	-0.4786365088106841
2216	80-81	-0.47362548221611434
2216	82-83	-0.43988076704274093
2216	84-85	-0.4438681491907026
2216	86-87	-0.3938124391341198
2216	88-89	-0.4184577371687759
2216	90-91	-0.3364281636595834
2216	92-93	-0.33285216413290897
2216	94-95	-0.3302463788332233
2216	96-97	-0.3279401968182363
2216	98-99	-0.37584324038342487
2216	100	-0.45956663908292583
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	3.0
3	12.0
4	25.0
5	634.0
6	4650.0
7	10502.0
8	15716.0
9	22032.0
10	31426.0
11	44521.0
12	52115.0
13	58271.0
14	63482.0
15	69292.0
16	75660.0
17	81740.0
18	90121.0
19	96474.0
20	104425.0
21	111918.0
22	119954.0
23	139856.0
24	223490.0
25	288126.0
26	198600.0
27	407859.0
28	241236.0
29	402158.0
30	325558.0
31	406701.0
32	526722.0
33	946523.0
34	1083985.0
35	3290707.0
36	2263727.0
37	2603668.0
38	1217291.0
39	284194.0
40	2062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.176457311209617	32.85117229017431	25.92184229197559	26.05052810664048
2	12.751253994798864	36.717356258757654	26.76124646966694	23.77014327677654
3	11.138831904502544	39.311633782402225	23.937983690977465	25.61155062211777
4	10.231298681032676	38.49909483127723	23.93830190926031	27.331304578429787
5	10.016303320348394	38.32236563938333	24.7548481960377	26.906482844230577
6	9.863469995369467	35.77053427805017	25.54995050390452	28.816045222675847
7	13.570647654293152	39.98281452379588	21.291474641991776	25.155063179919186
8	11.839603269670345	37.71934633754017	18.64340443874624	31.79764595404324
9	9.520447074557778	36.49585711882211	20.07088052603161	33.9128152805885
10-11	13.398833224250678	49.30304356327454	19.727145611827954	17.570977600646824
12-13	13.163169788648837	50.903347885814085	18.543449634246283	17.390032691290795
14-15	14.74792282954156	49.36235661000751	17.89668375767445	17.993036802776476
16-17	14.967351003153931	49.046464950284296	18.717131892918783	17.26905215364299
18-19	15.310206995380543	47.11314993823286	20.003868620077743	17.57277444630886
20-21	14.002073767929692	46.64656233653783	21.320947354128837	18.030416541403635
22-23	14.248663200706174	47.57195034767486	21.993134871953878	16.18625157966509
24-25	14.373548833283731	47.75527574167809	21.187124439853616	16.684050985184562
26-27	16.238564361406578	48.0993140424527	19.81206761400994	15.850053982130785
28-29	15.637839058507632	49.165138459484794	20.698212451099913	14.498810030907663
30-31	15.918233661576132	48.98565785176914	20.54831609686877	14.547792389785958
32-33	16.445810781483665	49.37543222228045	19.716239912874737	14.462517083361142
34-35	16.604256861685716	49.4652615985011	19.357941675848277	14.572539863964911
36-37	17.11244484166702	49.23254950621117	18.971850811147704	14.683154840974105
38-39	17.533609205647693	49.327617823366936	18.783699120270303	14.355073850715067
40-41	17.720129740151556	49.44556491702863	18.8135480629189	14.020757279900911
42-43	17.822297530378627	49.3797267988058	19.20500608451243	13.592969586303138
44-45	18.054114801484968	48.5810420667284	19.322384975167367	14.042458156619258
46-47	18.176475690468276	47.825777358713715	19.827307518200588	14.170439432617416
48-49	17.94987364714514	47.87254600806056	20.394648837212006	13.782931507582289
50-51	18.1369674839142	47.89029744782224	20.586745269404542	13.385989798859017
52-53	18.246338931650573	48.123753851011614	20.55622127214264	13.073685945195177
54-55	18.13179884926899	48.52307260603344	20.39803242545101	12.947096119246568
56-57	17.959533905458557	48.69885094792128	19.96797475303419	13.373640393585973
58-59	17.580390464986145	48.66572009974384	20.224088815110758	13.52980062015926
60-61	17.35620259781709	48.406810679368384	20.704387564567067	13.532599158247455
62-63	17.373683921621346	47.81200584281676	20.99495830381503	13.819351931746866
64-65	17.200414478424662	47.595199235958276	21.05036747877958	14.15401880683748
66-67	16.85885587756807	47.2700071431767	21.402036979591628	14.469099999663607
68-69	16.79569300059779	47.04187749680088	21.274873533358893	14.887555969242438
70-71	16.65916415596126	46.817402261694156	21.460895717406757	15.06253786493783
72-73	16.892922808898543	46.912821569853186	21.43880451114038	14.755451110107892
74-75	16.954413331968357	47.132309533517436	21.257263283183843	14.656013851330362
76-77	16.515414240105237	47.64419523242089	21.21560096361619	14.624789563857682
78-79	16.052459650999488	48.24190984928473	21.002194030304537	14.703436469411244
80-81	15.555635560864554	49.172940227072885	20.762043231480114	14.509380980582451
82-83	14.994558231326765	50.12917243926377	20.79887880762664	14.077390521782828
84-85	14.496691450563368	51.265369117754844	20.71797681300337	13.519962618678417
86-87	14.052944812706302	52.32766822852176	20.424408078716993	13.194978880054936
88-89	13.761631075018999	53.32743466899091	20.12542522421056	12.785509031779526
90-91	13.653169505373445	54.319498794101825	19.66952997719319	12.357801723331539
92-93	13.701005742857273	55.06590946286229	19.066688613691642	12.1663961805888
94-95	13.89939351548191	55.38553982265992	18.59209541814311	12.122971243715055
96-97	14.148132724973644	55.27856691700841	18.407462811586672	12.165837546431279
98-99	14.486049951048544	54.92934470824032	18.266524190018306	12.318081150692825
100	15.036943494168236	54.591138919083505	17.993793890404174	12.378123696344085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1921564.0
1	1602899.5
2	785187.5
3	185910.0
4	81605.0
5	71794.0
6	57371.0
7	41757.0
8	31225.0
9	27131.0
10	27937.0
11	31851.0
12	38931.0
13	50191.0
14	65705.5
15	85744.5
16	108904.0
17	133107.5
18	158549.0
19	182790.0
20	203428.5
21	220767.0
22	234969.5
23	245921.5
24	253493.5
25	257046.5
26	257299.0
27	253631.5
28	245362.5
29	233783.5
30	219940.0
31	204495.5
32	188952.0
33	174119.0
34	159934.5
35	147204.0
36	135863.5
37	126020.0
38	118744.5
39	114729.5
40	115256.0
41	122627.5
42	138121.0
43	164803.0
44	204230.5
45	253539.5
46	305596.0
47	351278.5
48	384387.0
49	404594.5
50	419414.5
51	439427.5
52	469779.5
53	504259.5
54	528326.0
55	526658.0
56	487128.5
57	412843.0
58	319225.5
59	223700.0
60	143381.0
61	85889.5
62	49297.5
63	28108.5
64	16883.5
65	11230.5
66	8251.0
67	6540.5
68	5401.0
69	4577.0
70	3973.0
71	3434.0
72	2955.5
73	2633.0
74	2341.0
75	2172.0
76	2036.5
77	1925.5
78	1799.5
79	1652.5
80	1604.0
81	1546.0
82	1461.5
83	1402.5
84	1334.5
85	1235.0
86	1168.0
87	1131.0
88	1061.0
89	1005.5
90	966.5
91	896.0
92	864.5
93	791.0
94	731.0
95	661.0
96	568.0
97	552.0
98	517.5
99	472.5
100	473.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.01189065097677E-4
2	4.275267633325636E-4
3	5.029726627441925E-5
4	7.230232026947766E-4
5	5.532699290186117E-4
6	8.927764763709416E-4
7	9.179251095081511E-4
8	0.001414610613968041
9	5.092598210284949E-4
10-11	4.212396050482612E-4
12-13	8.204741561014639E-4
14-15	0.002433130256025031
16-17	0.0012637188151447835
18-19	0.0034013526318076017
20-21	0.0035931109594788246
22-23	0.003536526534920103
24-25	0.00488826556604512
26-27	0.006915874112732647
28-29	0.009132097407949244
30-31	0.009484178271870179
32-33	0.009044077191969011
34-35	0.011439484498288226
36-37	0.01053099012620653
38-39	0.009185538253365814
40-41	0.007453426146040502
42-43	0.004473313119281161
44-45	0.00541009970364222
46-47	0.0076231794197166676
48-49	0.00608911279834688
50-51	0.004070934989085807
52-53	0.004322421320457904
54-55	0.00425954973761488
56-57	0.00697874569557567
58-59	0.008151300715598069
60-61	0.007783501955966377
62-63	0.009204399728218722
64-65	0.010018586726035884
66-67	0.009106948774812034
68-69	0.011543222609979217
70-71	0.011769560308214104
72-73	0.013740584430342907
74-75	0.017912113951977553
76-77	0.01916011487141158
78-79	0.01865714220866739
80-81	0.023353649447041287
82-83	0.021615250181431667
84-85	0.021234877105231375
86-87	0.021420348274618296
88-89	0.022206243060156096
90-91	0.021310323004643004
92-93	0.022064781998759293
94-95	0.019191550662833092
96-97	0.021634111656284576
98-99	0.018534542622123493
100	0.01913182265913222
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.5905437E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.33665505682025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.47639525723868	66.68293364420624
2	5.742621841906172	8.193188669156449
3	0.5651826809130189	1.209547258571428
4	0.09068794974613299	0.25877499955400557
5	0.03166126101276754	0.11293042277658748
6	0.019265948134797872	0.08246209778607991
7	0.010043719038828303	0.050153972439235896
8	0.008263336599596882	0.047158303409907266
9	0.005749733819394171	0.036915010032839
>10	0.03974268208955361	0.5573876660516196
>50	0.004827905178818417	0.24130890825018766
>100	0.004069438477030043	0.5606434296517188
>500	4.686444167178727E-4	0.23222584236210117
>1k	6.328699694236903E-4	0.9401814218605521
>5k	7.031481077891078E-5	0.3221917231638743
>10k+	3.1641664850509855E-4	20.471996630727176
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1386437	8.716748870213374	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	309551	1.9461961340640939	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	239316	1.5046175719661146	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	223849	1.407374094782809	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	104328	0.6559266494847014	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	100284	0.6305013813829825	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77002	0.4841237622078538	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72585	0.4563533840660901	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70431	0.4428108451217027	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68588	0.4312236124037334	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52930	0.33277928798812634	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42779	0.26895834424417264	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40729	0.25606966976135265	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38267	0.24059068606540013	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37307	0.23455501411246985	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34569	0.21734077473004984	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29349	0.18452180848599128	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28223	0.1774424682578668	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27980	0.1759146887947813	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27588	0.17345012274733476	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24379	0.15327463181300835	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23784	0.14953377263384843	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22648	0.1423915608228809	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19042	0.11972006804968639	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18873	0.11865753829963929	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18466	0.11609866487792822	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0023702586731820068	0.0	0.0	0.0	0.0
2	0.005413243282784371	0.0	0.0	1.2574316568604811E-5	0.0
3	0.009990294513756522	0.0	0.0	1.8861474852907217E-5	0.0
4	0.016139135315804274	0.0	0.0	2.5148633137209622E-5	0.0
5	0.025739626015934047	0.0	0.0	3.7722949705814434E-5	0.0
6	0.04377748313359765	0.0	0.0	4.4010107990116836E-5	0.0
7	0.06740462396600609	0.0	0.0	5.0297266274419245E-5	0.0
8	0.15732356174809908	0.0	0.0	5.0297266274419245E-5	0.0
9	0.4719203879780229	0.0	0.0	5.0297266274419245E-5	0.0
10-11	1.1525304208868956	0.0	0.0	5.0297266274419245E-5	0.0
12-13	2.0096241304152787	0.0	0.0	5.0297266274419245E-5	0.0
14-15	2.9074806306799363	0.0	0.0	5.3440845416570446E-5	0.0
16-17	3.777673634493664	0.0	0.0	5.972800370087285E-5	0.0
18-19	4.661201701028396	0.0	0.0	8.173305769593127E-5	0.0
20-21	5.766160338757118	0.0	0.0	9.430737426453609E-5	0.0
22-23	7.061490985755373	0.0	0.0	1.0059453254883849E-4	0.0
24-25	8.519750196112184	0.0	0.0	1.0059453254883849E-4	0.0
26-27	10.140023188297185	0.0	0.0	1.0059453254883849E-4	0.0
28-29	12.043076842214395	0.0	0.0	1.0688169083314089E-4	0.0
30-31	14.262500300997702	0.0	0.0	1.1945600740174571E-4	0.0
32-33	16.779262336520524	0.0	0.0	1.1945600740174571E-4	0.0
34-35	19.11100273447375	0.0	0.0	1.1945600740174571E-4	0.0
36-37	20.892871412461034	0.0	0.0	1.257431656860481E-4	0.0
38-39	22.20373134042152	0.0	0.0	1.6032253624971134E-4	0.0
40-41	23.363190209737713	0.0	0.0	1.8861474852907215E-4	0.0
42-43	24.28021311203207	0.0	0.0	2.294812773770378E-4	0.0
44-45	24.992331238682723	0.0	0.0	2.420555939456426E-4	0.0
46-47	25.5789576859787	0.0	0.0	2.4834275222994503E-4	0.0
48-49	26.108282972671546	0.0	0.0	2.609170687985498E-4	0.0
50-51	26.642820313582078	0.0	0.0	2.8292212279360825E-4	0.0
52-53	27.2208585026617	0.0	0.0	3.269322307837251E-4	0.0
54-55	27.83104293204896	0.0	0.0	3.898038136267492E-4	0.0
56-57	28.49122284411299	0.0	0.0	4.212396050482612E-4	0.0
58-59	29.198817360378087	0.0	0.0	4.4324465904331956E-4	0.0
60-61	29.93800484702181	0.0	0.0	4.715368713226804E-4	0.0
62-63	30.640950009735665	0.0	0.0	5.155469793127973E-4	0.0
64-65	31.300777840935773	3.143579142151203E-6	0.0	5.564135081607629E-4	0.0
66-67	31.896479801215143	6.287158284302406E-6	0.0	6.444337241409965E-4	0.0
68-69	32.416085141200455	1.5717895710756012E-5	0.0	7.104488861261718E-4	0.0
70-71	32.88684869205417	1.8861474852907217E-5	0.0	7.230232026947767E-4	0.0
72-73	33.32737729871867	2.200505399505842E-5	0.0	7.324539401212303E-4	0.0
74-75	33.7414621176394	2.5148633137209622E-5	0.0	7.701768898270448E-4	0.0
76-77	34.14064637142633	2.5148633137209622E-5	0.0	8.173305769593128E-4	0.0
78-79	34.52501179313715	2.5148633137209622E-5	0.0	8.959200555130927E-4	0.0
80-81	34.90123848844895	2.5148633137209622E-5	0.0	0.0010153760629148384	0.0
82-83	35.27575507670741	2.5148633137209622E-5	0.0	0.0010782476457578626	0.0
84-85	35.65190003896152	2.5148633137209622E-5	0.0	0.0011002526997529209	0.0
86-87	36.029821123430935	2.5148633137209622E-5	0.0	0.0011128270163215258	0.0
88	36.31615402959378	2.5148633137209622E-5	0.0	0.0011128270163215258	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	6010	0.0	43.63215	9
GCTTGCT	1230	0.0	37.823635	7
TACCCGA	9775	0.0	35.526966	6
ACCCGAC	10260	0.0	35.084442	7
CTTGCTG	3990	0.0	34.509083	8
CTACCCG	9635	0.0	33.214672	5
CCCGACG	12260	0.0	32.887848	8
GCCTGCT	1275	0.0	32.065815	7
CTGCTGA	6595	0.0	31.282146	9
TCGCGAC	810	0.0	30.168156	6
CCTGCTG	4855	0.0	29.522238	8
AGTTGCC	575	0.0	29.422075	3
TGAAAAA	13120	0.0	28.188992	1
ACTTGCC	1220	0.0	28.119116	3
GAAAAAA	98920	0.0	27.36384	1
GTACGAC	910	0.0	26.852976	6
GTTGCTG	1960	0.0	26.373957	8
ACTTTGC	775	0.0	26.073864	2
GCTGAAC	12600	0.0	26.033329	10-11
TGACGAC	1030	0.0	26.005672	6
>>END_MODULE