##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248477_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19430498
Sequences flagged as poor quality	0
Sequence length	100
%GC	25
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.56751715782066	31.0	30.0	34.0	16.0	34.0
2	30.11227478575176	33.0	30.0	34.0	23.0	34.0
3	30.63336945867265	33.0	31.0	34.0	26.0	34.0
4	34.21830593328076	37.0	35.0	37.0	30.0	37.0
5	34.128663866463945	37.0	35.0	37.0	30.0	37.0
6	33.96530063202703	37.0	35.0	37.0	28.0	37.0
7	34.21100025331312	37.0	35.0	37.0	30.0	37.0
8	34.125196482354696	37.0	35.0	37.0	30.0	37.0
9	35.55744021589153	39.0	35.0	39.0	28.0	39.0
10-11	36.245103393644364	39.0	37.0	39.0	31.0	39.0
12-13	36.324622122397486	39.0	37.0	39.0	31.0	39.0
14-15	37.65066165571258	40.0	38.0	41.0	31.0	41.0
16-17	37.46023436970066	40.0	38.0	41.0	31.0	41.0
18-19	36.635334848339966	39.0	36.0	41.0	30.5	41.0
20-21	36.1242983067135	38.5	35.0	41.0	30.0	41.0
22-23	35.79644319975741	38.0	35.0	41.0	29.0	41.0
24-25	35.55090559181757	38.0	35.0	41.0	27.0	41.0
26-27	35.398878042137675	37.5	35.0	40.0	25.0	41.0
28-29	35.26695996160263	37.0	35.0	40.0	25.0	41.0
30-31	35.20404788389881	37.0	35.0	40.0	25.0	41.0
32-33	35.12862107291332	37.0	34.5	40.0	25.0	41.0
34-35	35.026708425074844	37.0	34.0	40.0	25.0	41.0
36-37	34.934218232594965	37.0	34.0	40.0	25.0	41.0
38-39	34.80674224098631	36.5	34.0	40.0	25.0	41.0
40-41	34.67015160908382	36.0	33.0	40.0	25.0	41.0
42-43	34.50104320537744	35.5	33.0	40.0	25.0	41.0
44-45	34.3752400478876	35.0	33.0	40.0	25.0	41.0
46-47	34.29966987979412	35.0	33.0	40.0	25.0	41.0
48-49	34.30904542436329	35.0	33.0	40.0	25.0	41.0
50-51	34.182828098384306	35.0	33.0	39.0	25.0	41.0
52-53	33.9751837549403	35.0	33.0	39.0	25.0	41.0
54-55	33.92171613408982	35.0	33.0	39.0	25.0	41.0
56-57	33.87575472846862	35.0	33.0	39.0	25.0	41.0
58-59	33.672041138626504	35.0	33.0	38.0	25.0	41.0
60-61	33.469637782829864	35.0	33.0	37.0	25.0	40.0
62-63	33.225905095175634	35.0	32.5	37.0	25.0	40.0
64-65	33.039877233203185	35.0	32.0	36.0	25.0	40.0
66-67	32.86780920386086	35.0	32.0	36.0	25.0	39.0
68-69	32.68140178393781	35.0	32.0	35.0	25.0	39.0
70-71	32.51456681655817	35.0	31.5	35.0	25.0	39.0
72-73	32.35800497753583	35.0	31.0	35.0	25.0	37.5
74-75	32.196134731081	35.0	31.0	35.0	25.0	37.0
76-77	32.022586477196825	35.0	31.0	35.0	25.0	37.0
78-79	31.880979761815677	35.0	31.0	35.0	24.5	36.0
80-81	31.742156093992033	35.0	31.0	35.0	24.0	36.0
82-83	31.622900504145598	35.0	31.0	35.0	24.0	36.0
84-85	31.51013913796754	35.0	31.0	35.0	24.0	36.0
86-87	31.352101551900525	35.0	31.0	35.0	23.0	36.0
88-89	31.235024161501162	35.0	30.0	35.0	23.0	35.0
90-91	31.075663526482955	35.0	30.0	35.0	21.0	35.0
92-93	30.88407561658996	35.0	30.0	35.0	20.0	35.0
94-95	30.653195069935933	35.0	30.0	35.0	19.0	35.0
96-97	30.40095099466828	35.0	29.0	35.0	17.5	35.0
98-99	30.08703688397487	35.0	29.0	35.0	15.0	35.0
100	29.098186829797157	34.0	27.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.48302585486121075
1101	2	0.41565449071206473
1101	3	0.34708154157579685
1101	4	0.28287957569656186
1101	5	0.2680178177263741
1101	6	0.2993860695293975
1101	7	0.22518020562849017
1101	8	0.23352282258461088
1101	9	0.2959222338271701
1101	10-11	0.320649772554809
1101	12-13	0.3376318694668896
1101	14-15	0.4084680914016161
1101	16-17	0.4834355850729182
1101	18-19	0.45299652207035024
1101	20-21	0.4846100837474481
1101	22-23	0.5063660391076468
1101	24-25	0.5018485158852428
1101	26-27	0.5066541098580899
1101	28-29	0.4725726318464538
1101	30-31	0.45250687450032245
1101	32-33	0.4774766227197489
1101	34-35	0.47027043015005887
1101	36-37	0.4527288056919829
1101	38-39	0.47797095903050035
1101	40-41	0.460528600023423
1101	42-43	0.48024268211339205
1101	44-45	0.47956339727327446
1101	46-47	0.45244876638462017
1101	48-49	0.45371099611669763
1101	50-51	0.4383677744635648
1101	52-53	0.44481721561199095
1101	54-55	0.41429404608700793
1101	56-57	0.4297136377055466
1101	58-59	0.44401535040564966
1101	60-61	0.41621981143731546
1101	62-63	0.45256404525460425
1101	64-65	0.43583985255809665
1101	66-67	0.4349189663736084
1101	68-69	0.4521378158801497
1101	70-71	0.44681088645745604
1101	72-73	0.43283639495480486
1101	74-75	0.4447001299914035
1101	76-77	0.428344852973666
1101	78-79	0.47860031792821545
1101	80-81	0.4734878038524819
1101	82-83	0.42926544781203546
1101	84-85	0.4062008585464305
1101	86-87	0.4274287166659576
1101	88-89	0.3650160662516342
1101	90-91	0.3563736560046209
1101	92-93	0.32459939524179404
1101	94-95	0.3353474667296297
1101	96-97	0.32861938524237644
1101	98-99	0.28440838575144767
1101	100	0.294114204195818
1102	1	0.22766927880521592
1102	2	0.19518860860265974
1102	3	0.17223385585163697
1102	4	0.19454621434964992
1102	5	0.24369239365969975
1102	6	0.2458504795987153
1102	7	0.20274958937684318
1102	8	0.19836135229022034
1102	9	0.16967915760545793
1102	10-11	0.29311753555516873
1102	12-13	0.2799846919975977
1102	14-15	0.42848929031220706
1102	16-17	0.42216334965974056
1102	18-19	0.43458674482846504
1102	20-21	0.39832355108597284
1102	22-23	0.35674750007204636
1102	24-25	0.37220988521278286
1102	26-27	0.40648136778239063
1102	28-29	0.39880998297637404
1102	30-31	0.3773595436935224
1102	32-33	0.4018037143318338
1102	34-35	0.41196323496438225
1102	36-37	0.40721327540369145
1102	38-39	0.41916290677661294
1102	40-41	0.40103670302612215
1102	42-43	0.3883406585890654
1102	44-45	0.42499603094524474
1102	46-47	0.4262968360423116
1102	48-49	0.3998661988878638
1102	50-51	0.38717418939722137
1102	52-53	0.381483564545654
1102	54-55	0.3369262886303801
1102	56-57	0.3505876157995118
1102	58-59	0.3594506438936449
1102	60-61	0.3446524676675935
1102	62-63	0.3868986209166323
1102	64-65	0.37055250248975113
1102	66-67	0.3171993170269971
1102	68-69	0.30674417564178924
1102	70-71	0.35130689850271324
1102	72-73	0.29637292260332515
1102	74-75	0.33855617098795676
1102	76-77	0.3178031071317662
1102	78-79	0.3313422034251978
1102	80-81	0.3102915785796405
1102	82-83	0.2967708109388454
1102	84-85	0.2932999215068115
1102	86-87	0.2991228314839063
1102	88-89	0.2843104711723541
1102	90-91	0.268422328426162
1102	92-93	0.22934679749797837
1102	94-95	0.22712727731062898
1102	96-97	0.22313878052052516
1102	98-99	0.2072779593729095
1102	100	0.17380368541963875
1103	1	0.28103935822740667
1103	2	0.2651715181733003
1103	3	0.3125352204208127
1103	4	0.2637446965329602
1103	5	0.2066210869248053
1103	6	0.22573670997637407
1103	7	0.22101486082483035
1103	8	0.21444900986863757
1103	9	0.2855292482213798
1103	10-11	0.35011106917634294
1103	12-13	0.3542487707801456
1103	14-15	0.48291441224392884
1103	16-17	0.4586211333008947
1103	18-19	0.40970869320403835
1103	20-21	0.42451871512744077
1103	22-23	0.40402132672912217
1103	24-25	0.43638767898161035
1103	26-27	0.44634760935377926
1103	28-29	0.43120490848475157
1103	30-31	0.41505650006461536
1103	32-33	0.4385556332572378
1103	34-35	0.43704199086107565
1103	36-37	0.4499905701186506
1103	38-39	0.4716635564128211
1103	40-41	0.4703691768407907
1103	42-43	0.4718370006375139
1103	44-45	0.464301036808628
1103	46-47	0.47852545468253993
1103	48-49	0.42296897799822375
1103	50-51	0.43289258709450706
1103	52-53	0.4344446563342501
1103	54-55	0.4349432158178246
1103	56-57	0.41436226866057524
1103	58-59	0.4156370628323742
1103	60-61	0.3893740569109525
1103	62-63	0.40532247022768786
1103	64-65	0.3466956290055663
1103	66-67	0.3762396710953908
1103	68-69	0.41235738224609264
1103	70-71	0.388675238898756
1103	72-73	0.3831956685322737
1103	74-75	0.4227451751456286
1103	76-77	0.4056653376160284
1103	78-79	0.4299829312842611
1103	80-81	0.4298461780039595
1103	82-83	0.3940051430462006
1103	84-85	0.35715641187220726
1103	86-87	0.3739023049787491
1103	88-89	0.35913508605468536
1103	90-91	0.31702632685371057
1103	92-93	0.26538340033372165
1103	94-95	0.2660120684942342
1103	96-97	0.25705077313799407
1103	98-99	0.2616751136331956
1103	100	0.2305274201282046
1104	1	0.09887296595205797
1104	2	0.1239886765520204
1104	3	0.1335871806018929
1104	4	0.11743593969118393
1104	5	0.08204009172612103
1104	6	0.04763406088761002
1104	7	0.07139557917251693
1104	8	0.09183582955698455
1104	9	0.09139037878658485
1104	10-11	0.20188122973537048
1104	12-13	0.24557370565899106
1104	14-15	0.31092181778997485
1104	16-17	0.3298555748224601
1104	18-19	0.2848466660310507
1104	20-21	0.2611756658607831
1104	22-23	0.2619848757962444
1104	24-25	0.24848530661802926
1104	26-27	0.2625175657176868
1104	28-29	0.24653892463528848
1104	30-31	0.22410947812834792
1104	32-33	0.24279252652934247
1104	34-35	0.28357983368168505
1104	36-37	0.26340538433724703
1104	38-39	0.2904025802620609
1104	40-41	0.3018123688106442
1104	42-43	0.2656047133777051
1104	44-45	0.28420887635449077
1104	46-47	0.3120819491720326
1104	48-49	0.28329235164279254
1104	50-51	0.2922674111505543
1104	52-53	0.2870493770769471
1104	54-55	0.2733019502425691
1104	56-57	0.2823212483484454
1104	58-59	0.31106591640634207
1104	60-61	0.2665842262447171
1104	62-63	0.30416058954072156
1104	64-65	0.2737207606019467
1104	66-67	0.2602651295582987
1104	68-69	0.27448615582918734
1104	70-71	0.29001460896691356
1104	72-73	0.29787058013855017
1104	74-75	0.2856737919258947
1104	76-77	0.2694970429494248
1104	78-79	0.2685473687686404
1104	80-81	0.27280348461878745
1104	82-83	0.2754493300004306
1104	84-85	0.2605129397696935
1104	86-87	0.29425794044129816
1104	88-89	0.2616525336142388
1104	90-91	0.2671059436897316
1104	92-93	0.29189717837202167
1104	94-95	0.28851120907516403
1104	96-97	0.28689043455099394
1104	98-99	0.27970111401135256
1104	100	0.24511460142469588
1105	1	0.06815494587614879
1105	2	0.06835243323580542
1105	3	0.1530897307280803
1105	4	0.13155291172784445
1105	5	0.11835627596956044
1105	6	0.1308910146905049
1105	7	0.1700817210236849
1105	8	0.13757114950099236
1105	9	0.14599891997737302
1105	10-11	0.19554375036442906
1105	12-13	0.2159289286647521
1105	14-15	0.2727201845024183
1105	16-17	0.27142401879433464
1105	18-19	0.258292723999233
1105	20-21	0.22859233253036138
1105	22-23	0.2417735101554257
1105	24-25	0.2787698764078286
1105	26-27	0.28207556232966624
1105	28-29	0.26988233603831446
1105	30-31	0.23915613113671697
1105	32-33	0.25712199721655793
1105	34-35	0.27345191868642615
1105	36-37	0.24035277660061638
1105	38-39	0.27168343624888536
1105	40-41	0.26278256406016
1105	42-43	0.24492740066936136
1105	44-45	0.3000651649584256
1105	46-47	0.2850118305074645
1105	48-49	0.25001872867339614
1105	50-51	0.2087171844804132
1105	52-53	0.19521626549874327
1105	54-55	0.2534155186938136
1105	56-57	0.23872706275915334
1105	58-59	0.24666606088044318
1105	60-61	0.19058474832144867
1105	62-63	0.19211197450560746
1105	64-65	0.18916521073445125
1105	66-67	0.15237196222945926
1105	68-69	0.18905361626228512
1105	70-71	0.1547348481112465
1105	72-73	0.1499693861209508
1105	74-75	0.1846609672039463
1105	76-77	0.16454710557820107
1105	78-79	0.1861308500013159
1105	80-81	0.18633943358777216
1105	82-83	0.16127339289799636
1105	84-85	0.1668958836603025
1105	86-87	0.19196626326980848
1105	88-89	0.15450403079949382
1105	90-91	0.11342434049700145
1105	92-93	0.0767815421118172
1105	94-95	0.09494262873645809
1105	96-97	0.13784234732286293
1105	98-99	0.12826063968938328
1105	100	0.131151130499763
1106	1	0.025929128616272834
1106	2	0.012873056573255326
1106	3	0.08455990441262529
1106	4	0.08818702077660134
1106	5	0.12186247101652725
1106	6	0.06255961216628236
1106	7	0.03609499508670666
1106	8	0.041185640897637654
1106	9	0.0754535644792611
1106	10-11	0.1376886736428773
1106	12-13	0.15562696583263858
1106	14-15	0.19238034013699234
1106	16-17	0.18892732865263184
1106	18-19	0.15826319694618007
1106	20-21	0.12342553243794896
1106	22-23	0.10735746518502509
1106	24-25	0.14885311703673665
1106	26-27	0.16390898423109945
1106	28-29	0.1619373798989514
1106	30-31	0.14520413885739458
1106	32-33	0.15522093800507974
1106	34-35	0.1822060243035537
1106	36-37	0.14550097359349934
1106	38-39	0.14354959299311787
1106	40-41	0.15453034737819848
1106	42-43	0.1735476442629178
1106	44-45	0.15354125886548076
1106	46-47	0.17053684190882024
1106	48-49	0.16829037929247193
1106	50-51	0.18724806688074125
1106	52-53	0.1554107320345679
1106	54-55	0.13576368756567092
1106	56-57	0.13812191383079409
1106	58-59	0.154456071587326
1106	60-61	0.16505430422907352
1106	62-63	0.18008525634724748
1106	64-65	0.16516775058391175
1106	66-67	0.16000608216057088
1106	68-69	0.15213452296737984
1106	70-71	0.17993584318587352
1106	72-73	0.18465243147018384
1106	74-75	0.20349652096595605
1106	76-77	0.1783871644176287
1106	78-79	0.2101255458079372
1106	80-81	0.175898877628196
1106	82-83	0.16438263547085086
1106	84-85	0.15422751884254282
1106	86-87	0.16825835375948728
1106	88-89	0.1709822027763117
1106	90-91	0.16745049041366045
1106	92-93	0.13593453344565276
1106	94-95	0.15998435051408677
1106	96-97	0.17705814289621102
1106	98-99	0.18247609262074604
1106	100	0.15008095845896108
1107	1	0.010381474689474146
1107	2	0.04617058182538614
1107	3	0.0768691248098925
1107	4	0.09628761858609636
1107	5	0.09875929835342134
1107	6	0.056241211170096506
1107	7	0.04993939593752117
1107	8	0.045565227235172756
1107	9	0.04352388344324254
1107	10-11	0.03722403919024231
1107	12-13	0.0642621274639339
1107	14-15	0.08361439308261964
1107	16-17	0.09374770761794338
1107	18-19	0.04846508659898063
1107	20-21	0.04445320577737988
1107	22-23	0.04955205845949706
1107	24-25	0.06986170059247598
1107	26-27	0.08797719307115415
1107	28-29	0.07337171142845733
1107	30-31	0.07804208550221858
1107	32-33	0.07788724935691249
1107	34-35	0.0963534176570704
1107	36-37	0.08077474943164731
1107	38-39	0.08674272705540886
1107	40-41	0.06272680673571074
1107	42-43	0.057091597764376445
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2216	50-51	-0.20645994779840038
2216	52-53	-0.26148608262208484
2216	54-55	-0.23657553646512497
2216	56-57	-0.2562118463392835
2216	58-59	-0.24329378121592526
2216	60-61	-0.25560149305330526
2216	62-63	-0.22109474077451807
2216	64-65	-0.28259213999074007
2216	66-67	-0.3392616821761649
2216	68-69	-0.31909295142938277
2216	70-71	-0.4200481250506911
2216	72-73	-0.5323537583594344
2216	74-75	-0.5009051328860501
2216	76-77	-0.4359429673016635
2216	78-79	-0.43370879964495046
2216	80-81	-0.441571937673384
2216	82-83	-0.43316334793527744
2216	84-85	-0.4261522129467714
2216	86-87	-0.405031399339407
2216	88-89	-0.39197824253080427
2216	90-91	-0.3468048124723815
2216	92-93	-0.3857364157247396
2216	94-95	-0.3384419005468331
2216	96-97	-0.2995950689955187
2216	98-99	-0.3626692944882066
2216	100	-0.4147036325395348
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	1.0
2	4.0
3	11.0
4	33.0
5	457.0
6	2936.0
7	9002.0
8	17494.0
9	28213.0
10	40650.0
11	51171.0
12	56501.0
13	62347.0
14	70765.0
15	80973.0
16	92592.0
17	102345.0
18	112977.0
19	120573.0
20	128128.0
21	136126.0
22	145468.0
23	169595.0
24	270918.0
25	368913.0
26	242175.0
27	561063.0
28	293177.0
29	529492.0
30	362037.0
31	447476.0
32	583351.0
33	1176326.0
34	1245468.0
35	4486017.0
36	2175411.0
37	2860858.0
38	1917104.0
39	478885.0
40	3465.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.084772126166603	36.564015677029275	25.04501307148202	24.306199125322102
2	11.095396332787985	41.43297917526252	25.427591535552974	22.044032956396524
3	9.605825486555144	44.979676261214344	22.574959548091453	22.839538704139066
4	8.30358048564532	44.863307424214064	22.57806638922103	24.255045700919588
5	8.073867215868512	44.27322802092537	23.260205063421562	24.392699699784547
6	8.096450510817709	41.93725712246486	24.16947186193617	25.796820504781266
7	10.893419243061283	46.87724102096528	20.061565641900454	22.167774094072985
8	9.824007302432424	43.85023487617599	17.485107490705982	28.8406503306856
9	7.935137313944267	42.69002991755034	18.77848017650279	30.596352592002606
10-11	10.185394383733215	56.63047821518592	18.581357187617602	14.602770213463263
12-13	9.845240349324095	57.57789770144326	18.233067973188096	14.343793976044555
14-15	10.835310903707757	56.29016632898416	17.937513288094326	14.937009479213753
16-17	10.824703694918233	56.09226027055679	18.531837018682282	14.551199015842686
18-19	11.058836838270402	54.92829230259545	19.37906100135035	14.633809857783794
20-21	10.37503038832907	54.525809409083614	20.12129546421491	14.977864738372409
22-23	10.573516497585208	55.07031168074196	20.573677333520333	13.782494488152503
24-25	10.654727937298382	55.078986277683924	20.123106727109175	14.143179057908517
26-27	11.823046929545386	55.24445056814634	19.361689687422363	13.570812814885908
28-29	11.482117838329367	55.92504933514616	19.872429262571167	12.720403563953303
30-31	11.715751478118605	55.88934612472768	19.754348528660067	12.640553868493651
32-33	12.060934321423614	55.99047726228522	19.299651522481753	12.64893689380941
34-35	12.120604990136881	56.09410905069385	19.15927605072929	12.626009908439977
36-37	12.484439629261573	55.98988509632902	18.869430107871683	12.656245166537722
38-39	12.80780875143081	56.05898100142043	18.762490244439363	12.370720002709398
40-41	12.922631149393466	56.1943122149199	18.814981896363108	12.068074739323533
42-43	12.991244375309465	56.10393808691465	19.074462813215973	11.83035472455991
44-45	13.133138671517386	55.54745386796751	19.178583408745165	12.140824051769938
46-47	13.12647482401705	55.08165288684504	19.569568459293325	12.222303829844574
48-49	13.036864742330142	55.12604403198025	19.90593611423441	11.931155111455197
50-51	13.277762022965883	55.11014843777744	19.98583610958281	11.626253429673865
52-53	13.398312008243687	55.239975549747875	19.98919795622738	11.372514485781053
54-55	13.306235894452879	55.49130890753503	19.860484251517956	11.341970946494131
56-57	13.215377115158567	55.508764198633834	19.61283396840975	11.663024717797859
58-59	12.947011535245274	55.38335722644769	19.862152179674297	11.80747905863274
60-61	12.866699356697458	55.201394969087644	20.218906713539646	11.712998960675252
62-63	12.884791195519771	54.72993651541687	20.428458399640036	11.956813889423323
64-65	12.728429136368685	54.59913838351186	20.481592964432142	12.190839515687317
66-67	12.451365472622028	54.38126690072747	20.745687600246285	12.421680026404221
68-69	12.419661604963565	54.310954166365455	20.566778396806026	12.702605831864958
70-71	12.316919587542372	54.27941967279226	20.64244832022973	12.761212419435635
72-73	12.452988006076874	54.41180671673078	20.62089006166693	12.514315215525418
74-75	12.461136041747885	54.594977721742076	20.46578917722876	12.478097059281279
76-77	12.11403946991498	54.99479453401621	20.41971884563998	12.47144715042883
78-79	11.755810923020356	55.478491931710806	20.22493694642326	12.540760198845579
80-81	11.38174372531754	56.15019378675239	20.05234156000324	12.415720927926834
82-83	10.98849093125638	56.82363549952699	20.10077368440898	12.087099884807646
84-85	10.650546229473749	57.650927953983654	20.002220184493574	11.696305632049024
86-87	10.375720056561354	58.35850899025567	19.78803931579798	11.477731637384986
88-89	10.188193427467326	59.082718261024404	19.537054870525118	11.19203344098316
90-91	10.150333374797828	59.77245695562014	19.214966175807863	10.86224349377416
92-93	10.207043509997627	60.2184637068358	18.790393444698914	10.78409933846766
94-95	10.375420723420364	60.36760671856168	18.48948491294797	10.767487645069986
96-97	10.573336423320564	60.247078350840454	18.369416415553726	10.810168810285251
98-99	10.83366155302827	59.9635827347458	18.27856188890235	10.924193823323579
100	11.254823500197976	59.74751121699939	18.054365616424846	10.943299666377786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3027482.0
1	2504224.0
2	1207078.5
3	274376.0
4	108342.0
5	93550.0
6	75336.0
7	56648.0
8	44609.5
9	40680.0
10	43078.0
11	50119.0
12	62328.5
13	81003.5
14	106882.5
15	139869.0
16	178020.5
17	218565.5
18	259552.0
19	300770.5
20	338497.5
21	370441.5
22	396748.5
23	415603.0
24	428531.5
25	435818.5
26	434470.5
27	424274.0
28	407811.0
29	384550.0
30	356898.0
31	327125.5
32	296804.0
33	268162.5
34	240873.0
35	215854.0
36	194312.0
37	175438.5
38	159956.0
39	149962.0
40	144206.5
41	144533.5
42	154584.5
43	175194.0
44	207089.5
45	246633.0
46	287751.5
47	323203.5
48	344446.5
49	351307.5
50	351509.5
51	351181.5
52	354496.0
53	360273.0
54	359794.5
55	344205.0
56	307847.5
57	252998.0
58	189644.5
59	130138.0
60	82986.0
61	49656.0
62	28899.0
63	17525.5
64	11424.0
65	8226.0
66	6587.0
67	5639.5
68	4888.0
69	4269.0
70	3765.0
71	3385.0
72	3052.5
73	2770.0
74	2570.5
75	2391.5
76	2200.0
77	2050.5
78	1950.5
79	1846.0
80	1754.0
81	1680.5
82	1571.5
83	1482.5
84	1446.5
85	1426.0
86	1363.0
87	1265.0
88	1210.5
89	1113.5
90	1031.5
91	999.5
92	945.5
93	895.0
94	815.0
95	715.0
96	663.0
97	600.5
98	524.5
99	429.5
100	360.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.595430029636914E-4
2	4.992152028218731E-4
3	9.263787268859501E-5
4	7.462495299914598E-4
5	6.12439269441267E-4
6	9.315252753686499E-4
7	9.263787268859501E-4
8	0.001338102605501928
9	4.992152028218731E-4
10-11	4.220169755813773E-4
12-13	9.006459844724515E-4
14-15	0.0026298862746595587
16-17	0.001394714638811625
18-19	0.003679782165130302
20-21	0.0038727777332315414
22-23	0.003913950121093139
24-25	0.005226319984181569
26-27	0.007395590169639502
28-29	0.009801601585301623
30-31	0.009855640344369969
32-33	0.00947736903089154
34-35	0.01190653991472581
36-37	0.010998174107529308
38-39	0.009443916465753991
40-41	0.007869072630047876
42-43	0.004495510099638208
44-45	0.005535112893143552
46-47	0.007642624496809088
48-49	0.006464064894270852
50-51	0.004158411174021376
52-53	0.004330820548191817
54-55	0.004444044614811211
56-57	0.006896374966817629
58-59	0.008280796508663854
60-61	0.007910245017909474
62-63	0.009490235402098288
64-65	0.010378015015363992
66-67	0.009189162315860356
68-69	0.011772729654175616
70-71	0.011942565754104708
72-73	0.01411698248804534
74-75	0.018514708166512253
76-77	0.019572323879707046
78-79	0.019376755037364458
80-81	0.023851678943071865
82-83	0.02210699900743666
84-85	0.02153573212585699
86-87	0.021407068413789497
88-89	0.02261650730722393
90-91	0.021818792292405475
92-93	0.022583054742086384
94-95	0.019762746173566936
96-97	0.022191917057401207
98-99	0.01916317327533242
100	0.019217212034400764
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9430498E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.96731879371827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.6764580971731	53.45398571514435
2	10.040579983296988	12.242944814309881
3	1.7748642802562657	3.246261491699013
4	0.3075278864891666	0.7499660277417373
5	0.07882215474381567	0.24027877181370016
6	0.026607307531025075	0.0973305720291904
7	0.015031324862782249	0.06414937023608323
8	0.008549896059910434	0.04170113909901722
9	0.008831588392387589	0.04845944384762364
>10	0.04847982364782965	0.5769355919380124
>50	0.007328307730558844	0.3150480005514319
>100	0.005202795977200054	0.6167551525845397
>500	5.306957226045349E-4	0.21834487647942502
>1k	7.2848258075411E-4	0.9027449619551429
>5k	1.439885945749437E-4	0.6538764947967262
>10k+	3.1338694113370094E-4	26.531217575774065
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2252583	11.59302762080519	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	464669	2.3914415369075974	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	373867	1.9241246415814972	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	333093	1.714279273747899	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	157033	0.8081779478837856	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	153909	0.7921001304238318	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	126329	0.6501583232709732	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	117338	0.60388570586302	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115316	0.5934793848310013	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	103535	0.5328478971563159	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93839	0.4829469630680593	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64992	0.3344844789876204	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64528	0.33209648049164775	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58166	0.2993541390447121	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58116	0.2990968116205771	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52166	0.26847484814851375	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48230	0.24821803332060766	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45249	0.23287617229367977	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43586	0.22431746216695012	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42491	0.2186819915783939	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37356	0.19225446511973085	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36134	0.1859653828738718	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30284	0.15585807425007842	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28989	0.14919329396498227	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27643	0.14226603970726845	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23534	0.12111887199185527	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22074	0.11360491120711368	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22060	0.11353285952835589	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21334	0.10979646532991588	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19704	0.10140759130311534	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0018424643568065008	0.0	0.0	1.0293096965399446E-5	0.0
2	0.004302514531536968	0.0	0.0	2.0586193930798892E-5	0.0
3	0.007004452484954323	0.0	0.0	7.205167875779612E-5	0.0
4	0.011116544722631402	0.0	0.0	8.234477572319557E-5	0.0
5	0.017400480420007764	0.0	0.0	9.778442117129474E-5	0.0
6	0.02932503325442302	0.0	0.0	9.778442117129474E-5	0.0
7	0.04664831544719029	0.0	0.0	9.778442117129474E-5	0.0
8	0.10736729444608162	0.0	0.0	9.778442117129474E-5	0.0
9	0.3325184974672291	0.0	0.0	1.0807751813669418E-4	0.0
10-11	0.7867914656639269	0.0	0.0	1.132240666193939E-4	0.0
12-13	1.3338000909703909	0.0	0.0	1.2866371206749307E-4	0.0
14-15	1.9155273323411475	0.0	0.0	1.2866371206749307E-4	0.0
16-17	2.49915365010202	0.0	0.0	1.3895680903289252E-4	0.0
18-19	3.1077252883585382	0.0	0.0	1.4924990599829197E-4	0.0
20-21	3.876653084239014	0.0	0.0	1.4924990599829197E-4	0.0
22-23	4.769823707040345	0.0	0.0	1.543964544809917E-4	0.0
24-25	5.720898146820529	0.0	0.0	1.543964544809917E-4	0.0
26-27	6.749474460201689	0.0	0.0	1.6468955144639114E-4	0.0
28-29	7.964553970773164	0.0	0.0	1.878490196185399E-4	0.0
30-31	9.39551575054844	5.146548482699723E-6	0.0	2.0586193930798892E-4	0.0
32-33	10.994563803768695	5.146548482699723E-6	7.719822724049584E-6	2.3159468172148753E-4	0.0
34-35	12.460249346156749	5.146548482699723E-6	1.543964544809917E-5	2.6247397261768587E-4	0.0
36-37	13.540085282425597	5.146548482699723E-6	1.543964544809917E-5	2.7019379534173546E-4	0.0
38-39	14.330456172559241	5.146548482699723E-6	1.543964544809917E-5	2.9849981199658393E-4	0.0
40-41	15.041773504724377	5.146548482699723E-6	1.543964544809917E-5	3.4481874834088144E-4	0.0
42-43	15.603938715312392	5.146548482699723E-6	1.543964544809917E-5	4.5032299223622576E-4	0.0
44-45	16.03802692035994	5.146548482699723E-6	1.543964544809917E-5	5.275212194767216E-4	0.0
46-47	16.392850558951192	5.146548482699723E-6	1.543964544809917E-5	5.686936073383194E-4	0.0
48-49	16.709738988676463	5.146548482699723E-6	1.543964544809917E-5	6.51038383061515E-4	0.0
50-51	17.03110491558168	5.146548482699723E-6	1.543964544809917E-5	7.025038678885122E-4	0.0
52-53	17.375663763224185	5.146548482699723E-6	1.543964544809917E-5	7.79702095129008E-4	0.0
54-55	17.73576004073596	5.146548482699723E-6	1.543964544809917E-5	9.212321784032504E-4	0.0
56-57	18.120214932216353	5.146548482699723E-6	1.543964544809917E-5	9.701243889888978E-4	0.0
58-59	18.537471350451234	1.0293096965399446E-5	1.543964544809917E-5	0.001006150228367796	0.0
60-61	18.978430197723185	1.0293096965399446E-5	1.543964544809917E-5	0.0010730553586428921	0.0
62-63	19.39426616857684	1.2866371206749307E-5	1.543964544809917E-5	0.0011399604889179888	0.0
64-65	19.788036312810924	1.543964544809917E-5	1.543964544809917E-5	0.0012223052646411843	0.0
66-67	20.144712708855945	1.543964544809917E-5	1.543964544809917E-5	0.0013535422509500271	0.0
68-69	20.45812207180691	1.543964544809917E-5	1.543964544809917E-5	0.001410154284259724	0.0
70-71	20.74622585586844	1.543964544809917E-5	1.543964544809917E-5	0.0014616197690867213	0.0
72-73	21.022333035416796	1.543964544809917E-5	1.543964544809917E-5	0.00148477923725887	0.0
74-75	21.28682188176546	1.543964544809917E-5	1.543964544809917E-5	0.0015516843675339666	0.0
76-77	21.545914572030014	1.543964544809917E-5	1.543964544809917E-5	0.00167520153111876	0.0
78-79	21.798411960413986	1.543964544809917E-5	1.543964544809917E-5	0.0018295979855997516	0.0
80-81	22.045852864913705	1.543964544809917E-5	1.543964544809917E-5	0.0020689124900452886	0.0
82-83	22.296541241506006	1.543964544809917E-5	1.543964544809917E-5	0.0021975762021127815	0.0
84-85	22.550495103110585	1.543964544809917E-5	1.543964544809917E-5	0.0022567615096638284	0.0
86-87	22.80498420575736	1.543964544809917E-5	1.543964544809917E-5	0.0022902140748013765	0.0
88	22.997418800073987	1.543964544809917E-5	1.543964544809917E-5	0.002300507171766776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	3670	0.0	39.822964	9
CCCGACG	9805	0.0	33.261955	8
ACTTGCC	1060	0.0	31.919235	3
TGAAAAA	18485	0.0	31.853683	1
GCCTGCT	945	0.0	31.825737	7
TACCCGA	9350	0.0	31.009829	6
CTTGCTG	2570	0.0	30.536499	8
CTACCCG	8890	0.0	30.288486	5
TGCCAAA	2050	0.0	29.570707	6
CTGCTGA	4670	0.0	29.484243	9
ACATGCC	1055	0.0	29.397966	3
ACCCGAC	10125	0.0	29.100658	7
GTTTGCC	570	0.0	28.854862	3
GAAAAAA	148565	0.0	28.515257	1
ACTAGCC	710	0.0	28.460068	3
CACTGCC	1240	0.0	28.422705	3
ACTGCTG	795	0.0	27.7822	8
CCGACGC	11945	0.0	27.499846	9
GCTTGCT	735	0.0	27.492321	7
ATTTGCC	1030	0.0	27.374098	3
>>END_MODULE