##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248477_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19430498
Sequences flagged as poor quality	0
Sequence length	100
%GC	26
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47138874155464	34.0	31.0	34.0	31.0	34.0
2	32.71446094690934	34.0	31.0	34.0	31.0	34.0
3	32.83342907629027	34.0	31.0	34.0	31.0	34.0
4	36.282965830314794	37.0	37.0	37.0	35.0	37.0
5	36.21008030777183	37.0	37.0	37.0	35.0	37.0
6	36.20104322596364	37.0	37.0	37.0	35.0	37.0
7	36.189483100227285	37.0	37.0	37.0	35.0	37.0
8	36.182364960486346	37.0	37.0	37.0	35.0	37.0
9	37.93810863725675	39.0	39.0	39.0	35.0	39.0
10-11	37.978956355107314	39.0	39.0	39.0	35.0	39.0
12-13	37.95710300374185	39.0	38.5	39.0	35.0	39.0
14-15	39.43429378392669	41.0	39.0	41.0	36.0	41.0
16-17	39.35136621305331	41.0	39.0	41.0	36.0	41.0
18-19	39.00620241436941	40.5	38.5	41.0	35.5	41.0
20-21	38.553627755706515	40.0	37.0	41.0	35.0	41.0
22-23	38.007173156344216	39.5	35.5	41.0	34.5	41.0
24-25	37.517967655795545	39.0	35.0	41.0	34.0	41.0
26-27	37.309742421424296	39.0	35.0	41.0	33.0	41.0
28-29	37.158733502352845	39.0	35.0	41.0	33.0	41.0
30-31	37.07989128739778	39.0	35.0	41.0	33.0	41.0
32-33	37.00156967155448	39.0	35.0	41.0	33.0	41.0
34-35	36.951374946746085	39.0	35.0	41.0	33.0	41.0
36-37	36.85934359479618	38.5	35.0	41.0	33.0	41.0
38-39	36.79405270518542	38.0	35.0	41.0	33.0	41.0
40-41	36.67639910721795	38.0	35.0	41.0	33.0	41.0
42-43	36.551416412487214	38.0	35.0	41.0	33.0	41.0
44-45	36.3884203585518	38.0	35.0	41.0	32.0	41.0
46-47	36.201018676927376	37.0	35.0	40.5	32.0	41.0
48-49	35.97720812405323	36.5	35.0	40.0	31.0	41.0
50-51	35.73008823551511	36.0	35.0	40.0	31.0	41.0
52-53	35.47714371499897	35.0	35.0	40.0	31.0	41.0
54-55	35.26069897951149	35.0	35.0	39.0	31.0	41.0
56-57	35.027874144038925	35.0	35.0	39.0	30.0	41.0
58-59	34.797667332046764	35.0	35.0	39.0	30.0	41.0
60-61	34.57530944394735	35.0	35.0	38.0	29.5	41.0
62-63	34.3582343592017	35.0	34.5	37.0	29.0	40.0
64-65	34.15026470242811	35.0	34.0	36.5	29.0	40.0
66-67	33.9402180788161	35.0	34.0	36.0	29.0	40.0
68-69	33.72846545158029	35.0	34.0	36.0	29.0	39.0
70-71	33.567543328019696	35.0	34.0	35.0	28.5	39.0
72-73	33.405634688313185	35.0	34.0	35.0	28.0	38.5
74-75	33.25134577096274	35.0	34.0	35.0	27.0	37.0
76-77	33.09900690656514	35.0	33.0	35.0	27.0	37.0
78-79	32.93029447829901	35.0	33.0	35.0	27.0	36.5
80-81	32.777835081735944	35.0	33.0	35.0	27.0	36.0
82-83	32.6663156034395	35.0	33.0	35.0	27.0	36.0
84-85	32.54525728059055	35.0	33.0	35.0	27.0	36.0
86-87	32.426224047371306	35.0	33.0	35.0	26.0	35.0
88-89	32.289264819666485	35.0	33.0	35.0	25.0	35.0
90-91	32.19129890546294	35.0	33.0	35.0	25.0	35.0
92-93	32.0639801151777	35.0	33.0	35.0	24.5	35.0
94-95	31.885586488828025	35.0	33.0	35.0	24.0	35.0
96-97	31.75414122170209	35.0	33.0	35.0	24.0	35.0
98-99	31.61585467341084	35.0	33.0	35.0	23.0	35.0
100	30.73163374402447	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2914683484077898
1101	2	0.260052676980024
1101	3	0.26127523677875075
1101	4	0.17267782326978676
1101	5	0.17594463554999606
1101	6	0.1821811636902737
1101	7	0.20279764397262312
1101	8	0.20114774996145002
1101	9	0.2840596789860257
1101	10-11	0.26532006878706227
1101	12-13	0.27153581542526695
1101	14-15	0.3714500962370906
1101	16-17	0.3838885509095107
1101	18-19	0.40812616336216934
1101	20-21	0.380707080923294
1101	22-23	0.37959659186480366
1101	24-25	0.397556015421074
1101	26-27	0.43903167046740066
1101	28-29	0.43949735810311097
1101	30-31	0.4529411114138071
1101	32-33	0.4691633214963886
1101	34-35	0.5164045934920978
1101	36-37	0.5386052977753124
1101	38-39	0.5373525282193867
1101	40-41	0.5160981119345749
1101	42-43	0.5525855047847728
1101	44-45	0.5419684909513407
1101	46-47	0.5356694600564822
1101	48-49	0.5425148459656697
1101	50-51	0.5397468936623753
1101	52-53	0.5022814842858025
1101	54-55	0.5376115081332316
1101	56-57	0.5328128623190906
1101	58-59	0.5609400889305292
1101	60-61	0.566564181231783
1101	62-63	0.5694731797342172
1101	64-65	0.5532878270331594
1101	66-67	0.57388082908831
1101	68-69	0.6322524529189621
1101	70-71	0.5951321826171068
1101	72-73	0.5932515519206802
1101	74-75	0.5681416237802139
1101	76-77	0.5953709664943858
1101	78-79	0.6297302950777706
1101	80-81	0.638054514360249
1101	82-83	0.6350252186846674
1101	84-85	0.5803100947443625
1101	86-87	0.5438182356017691
1101	88-89	0.5749928398768489
1101	90-91	0.6248033164492952
1101	92-93	0.652506684373364
1101	94-95	0.6750550427953463
1101	96-97	0.7209148573842228
1101	98-99	0.684907037374348
1101	100	0.6434963878625553
1102	1	0.2485036767800679
1102	2	0.23698830500917722
1102	3	0.23192509883396895
1102	4	0.13456680573386848
1102	5	0.15880978376249288
1102	6	0.15376316741048157
1102	7	0.1738876554493487
1102	8	0.1623496742292332
1102	9	0.25977007249725403
1102	10-11	0.23677774232679383
1102	12-13	0.23931678429420344
1102	14-15	0.32577759257173255
1102	16-17	0.3089435710615973
1102	18-19	0.33845184117292604
1102	20-21	0.29018746876102597
1102	22-23	0.2701819740231599
1102	24-25	0.2917795496619249
1102	26-27	0.29773646244147756
1102	28-29	0.32772070568503153
1102	30-31	0.33303664879100836
1102	32-33	0.34400900719813876
1102	34-35	0.37979794150454893
1102	36-37	0.4304503991726847
1102	38-39	0.4023818945639235
1102	40-41	0.42708035663056876
1102	42-43	0.4327378736133838
1102	44-45	0.4255465221979833
1102	46-47	0.4166923590967002
1102	48-49	0.43580864844690836
1102	50-51	0.4520739414848052
1102	52-53	0.4513279358199469
1102	54-55	0.45725328738501503
1102	56-57	0.4519629117704369
1102	58-59	0.47652841914992905
1102	60-61	0.48321559818831616
1102	62-63	0.43741713974025487
1102	64-65	0.4617377285727855
1102	66-67	0.4958205869094101
1102	68-69	0.49121224148423437
1102	70-71	0.48532634623766313
1102	72-73	0.4592695493210144
1102	74-75	0.4577359974141828
1102	76-77	0.4237817278976763
1102	78-79	0.4812337796726567
1102	80-81	0.47119293950146357
1102	82-83	0.4486593677735513
1102	84-85	0.3058844845200497
1102	86-87	0.3276255137961144
1102	88-89	0.39077631345502795
1102	90-91	0.3869870047589643
1102	92-93	0.4356207524410607
1102	94-95	0.458912269208799
1102	96-97	0.46175286456277576
1102	98-99	0.45922275842039184
1102	100	0.41883398987623366
1103	1	0.24129218188387114
1103	2	0.23420802863061851
1103	3	0.19829579790915375
1103	4	0.12503401076571663
1103	5	0.12172658368787381
1103	6	0.13675036124859474
1103	7	0.16020731643919106
1103	8	0.16352901379907792
1103	9	0.22137780550010433
1103	10-11	0.20791430585121873
1103	12-13	0.2057702703420219
1103	14-15	0.26676062540668255
1103	16-17	0.2783137570907641
1103	18-19	0.27892872411847947
1103	20-21	0.26693792184064336
1103	22-23	0.25746179732210805
1103	24-25	0.23637684184497232
1103	26-27	0.25574021367412314
1103	28-29	0.2606734188323401
1103	30-31	0.23511926585878484
1103	32-33	0.24113619930388808
1103	34-35	0.28779494651507775
1103	36-37	0.3177840256751807
1103	38-39	0.29804850459563426
1103	40-41	0.3145154596395514
1103	42-43	0.2803303884717252
1103	44-45	0.3068416136134573
1103	46-47	0.27840151863402696
1103	48-49	0.2882803476286995
1103	50-51	0.295526424760844
1103	52-53	0.27382397137336767
1103	54-55	0.281552263291843
1103	56-57	0.3153677883728747
1103	58-59	0.335557846004221
1103	60-61	0.3294700623550497
1103	62-63	0.3428197309558385
1103	64-65	0.3296420188369069
1103	66-67	0.335292549277149
1103	68-69	0.3358962351144612
1103	70-71	0.32854807540266506
1103	72-73	0.33590113563268176
1103	74-75	0.3108462223548827
1103	76-77	0.3051091512072901
1103	78-79	0.35467901700642557
1103	80-81	0.3892143475129828
1103	82-83	0.36459657627012376
1103	84-85	0.3584764163715448
1103	86-87	0.37334636012217004
1103	88-89	0.359151123561368
1103	90-91	0.4064974788934492
1103	92-93	0.41740078895394106
1103	94-95	0.4429280370454727
1103	96-97	0.3968263567404904
1103	98-99	0.39019071636394287
1103	100	0.39767743754553564
1104	1	0.21631779355760017
1104	2	0.2024156409174367
1104	3	0.19254868322230578
1104	4	0.11617124509204757
1104	5	0.11364500656487309
1104	6	0.11937393678796582
1104	7	0.12424826389413823
1104	8	0.14864357309197374
1104	9	0.19052195600582422
1104	10-11	0.17349829164371755
1104	12-13	0.17867057992161506
1104	14-15	0.2506923131448886
1104	16-17	0.239796246120612
1104	18-19	0.23468570319997184
1104	20-21	0.19901787550351457
1104	22-23	0.1638344607313087
1104	24-25	0.1588552237911003
1104	26-27	0.18148884662954856
1104	28-29	0.1877499417911821
1104	30-31	0.18121693215175583
1104	32-33	0.19097430832109552
1104	34-35	0.22016622405467956
1104	36-37	0.2567884651193282
1104	38-39	0.25440060149953325
1104	40-41	0.27293949879970825
1104	42-43	0.2656314732605267
1104	44-45	0.2602241054657455
1104	46-47	0.2536950919722756
1104	48-49	0.3008283627019708
1104	50-51	0.31751464374740834
1104	52-53	0.292294157835542
1104	54-55	0.2721986447748108
1104	56-57	0.311995411233994
1104	58-59	0.3163885716187522
1104	60-61	0.31047172555163627
1104	62-63	0.3063701086504409
1104	64-65	0.3034720018722652
1104	66-67	0.33099707643090426
1104	68-69	0.3383596051682787
1104	70-71	0.31536634099244765
1104	72-73	0.3078013365083905
1104	74-75	0.3068001141917165
1104	76-77	0.2817186005581931
1104	78-79	0.3292231044706
1104	80-81	0.34295625519180817
1104	82-83	0.29976628668731564
1104	84-85	0.29403192569021286
1104	86-87	0.27734777078774897
1104	88-89	0.2814457636612602
1104	90-91	0.32149294138724116
1104	92-93	0.3225248544366224
1104	94-95	0.3483283666505663
1104	96-97	0.37338925204102225
1104	98-99	0.3628868680453614
1104	100	0.3650551710030001
1105	1	0.16930717696315156
1105	2	0.16914419988727758
1105	3	0.15075383813731946
1105	4	0.10010522054482607
1105	5	0.09950573803173768
1105	6	0.07982275222276769
1105	7	0.11130539998275424
1105	8	0.10378372910360412
1105	9	0.12648226408555274
1105	10-11	0.13418089306303926
1105	12-13	0.14616737884064435
1105	14-15	0.18709439835041053
1105	16-17	0.19146742223662017
1105	18-19	0.1860273989967567
1105	20-21	0.17585102882207337
1105	22-23	0.14078417981369284
1105	24-25	0.13231270477949408
1105	26-27	0.13274794222499509
1105	28-29	0.17214980935708013
1105	30-31	0.15389560196386043
1105	32-33	0.1502748616813676
1105	34-35	0.19264451640702873
1105	36-37	0.2085709900786057
1105	38-39	0.21824014926642832
1105	40-41	0.1998070790478721
1105	42-43	0.20385447106798438
1105	44-45	0.219826495836017
1105	46-47	0.2019480772935509
1105	48-49	0.20281606413836073
1105	50-51	0.2125226200529866
1105	52-53	0.22098394710977232
1105	54-55	0.23208461605553055
1105	56-57	0.20965934465061054
1105	58-59	0.22856269949264885
1105	60-61	0.2497535796301662
1105	62-63	0.23096115316147348
1105	64-65	0.22438160155912357
1105	66-67	0.23579129877769134
1105	68-69	0.23919112360360373
1105	70-71	0.23836399908096695
1105	72-73	0.25051614163233893
1105	74-75	0.22274834499419427
1105	76-77	0.22041160581352415
1105	78-79	0.23199381859650714
1105	80-81	0.24253376181272301
1105	82-83	0.244234330574713
1105	84-85	0.21827130454329335
1105	86-87	0.2257952397099956
1105	88-89	0.2228882047010785
1105	90-91	0.2730353293825871
1105	92-93	0.31265357896007373
1105	94-95	0.28751196976608995
1105	96-97	0.28856905679874956
1105	98-99	0.30065117526326546
1105	100	0.2534766058805147
1106	1	0.09287768407575925
1106	2	0.10284386009666235
1106	3	0.10419064899108577
1106	4	0.0759152141304753
1106	5	0.09383599193629522
1106	6	0.06877866841995228
1106	7	0.08702419539311279
1106	8	0.0870480793493229
1106	9	0.12025654441444544
1106	10-11	0.10791628250147056
1106	12-13	0.11985514987775758
1106	14-15	0.14930518585082808
1106	16-17	0.12783343900267852
1106	18-19	0.14485839005688916
1106	20-21	0.13540368326940921
1106	22-23	0.09344742510487691
1106	24-25	0.07111352893566902
1106	26-27	0.08768811442627111
1106	28-29	0.10400519484602455
1106	30-31	0.1160498223086961
1106	32-33	0.10424775745948267
1106	34-35	0.12924324993328895
1106	36-37	0.16533086119170548
1106	38-39	0.15328697760003251
1106	40-41	0.12803934409558337
1106	42-43	0.10509492029272138
1106	44-45	0.13021962603524173
1106	46-47	0.1462746529184571
1106	48-49	0.1670939976915946
1106	50-51	0.15851151579136058
1106	52-53	0.14713758077115813
1106	54-55	0.15584681651318277
1106	56-57	0.18943515700623692
1106	58-59	0.1867950626469863
1106	60-61	0.1855104145945745
1106	62-63	0.19090356211974324
1106	64-65	0.19349626426957656
1106	66-67	0.2000465271895706
1106	68-69	0.20756943989715637
1106	70-71	0.1879162474118985
1106	72-73	0.20733709143976142
1106	74-75	0.190210249511928
1106	76-77	0.18218299809112182
1106	78-79	0.2284725131613925
1106	80-81	0.252761697049138
1106	82-83	0.24471406831620612
1106	84-85	0.19576285776439306
1106	86-87	0.18123646303205732
1106	88-89	0.22932881015511697
1106	90-91	0.21604069426445704
1106	92-93	0.23505210255012088
1106	94-95	0.2580035976148771
1106	96-97	0.25362311167398843
1106	98-99	0.204520136744879
1106	100	0.1395460689618062
1107	1	0.08733334886931488
1107	2	0.09440526882475808
1107	3	0.09152573377806306
1107	4	0.06884419501125905
1107	5	0.056976748347885575
1107	6	0.04978256522974789
1107	7	0.0731302336549362
1107	8	0.0626596790636782
1107	9	0.09273347468291604
1107	10-11	0.10733250240598835
1107	12-13	0.0747038438440839
1107	14-15	0.10224714597077167
1107	16-17	0.1174211409377861
1107	18-19	0.13314909762630123
1107	20-21	0.13707952595526507
1107	22-23	0.12346309459787363
1107	24-25	0.10398253162051674
1107	26-27	0.11684687481642442
1107	28-29	0.12524418891690914
1107	30-31	0.1442878747227212
1107	32-33	0.13043164032239218
1107	34-35	0.1946241267129949
1107	36-37	0.197342229271257
1107	38-39	0.2161039059849159
1107	40-41	0.21343189148937824
1107	42-43	0.18739524013501807
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2216	64-65	-0.6035176236735751
2216	66-67	-0.5876733431100405
2216	68-69	-0.6859865298720251
2216	70-71	-0.6349687743416794
2216	72-73	-0.6576530115545651
2216	74-75	-0.6497858275641377
2216	76-77	-0.7212421477497486
2216	78-79	-0.6560846189305423
2216	80-81	-0.6394161971082539
2216	82-83	-0.6807347473920302
2216	84-85	-0.6963025980223101
2216	86-87	-0.6982225404118623
2216	88-89	-0.7342351995872285
2216	90-91	-0.832033595676954
2216	92-93	-0.8862535884153004
2216	94-95	-0.8855693655846153
2216	96-97	-0.8832078797715184
2216	98-99	-0.9331220645189795
2216	100	-0.9913065102526666
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	11.0
6	292.0
7	932.0
8	2006.0
9	3219.0
10	4836.0
11	7012.0
12	9612.0
13	13144.0
14	18157.0
15	23804.0
16	31284.0
17	40663.0
18	51470.0
19	61898.0
20	74348.0
21	87623.0
22	102387.0
23	119440.0
24	137262.0
25	157617.0
26	181428.0
27	208806.0
28	244356.0
29	292960.0
30	339973.0
31	413316.0
32	524103.0
33	736858.0
34	1110795.0
35	4934564.0
36	2213975.0
37	4109931.0
38	2535634.0
39	632330.0
40	4452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.019086927394625	22.465074906670814	35.92768717835979	23.588150987574767
2	14.352202435494007	22.095309847667234	44.09966915331381	19.452818563524946
3	11.25387522234376	17.41991893362692	51.0659428286398	20.26026301538952
4	11.166229501683384	15.58102113491893	54.92085174553941	18.331897617858274
5	10.346887101839416	15.98239749292012	56.3577955672998	17.31291983794066
6	10.23799287079518	12.494651449489353	52.39199736414373	24.875358315571734
7	13.75727992149249	13.677611350980298	49.58889885375043	22.97620987377678
8	11.208456931983935	12.634339068406792	50.73550353675959	25.42170046284969
9	11.737619900426639	13.081970415786564	52.56306863570867	22.617341048078128
10-11	13.762284939891916	20.924839394234777	53.2204681526948	12.09240751317851
12-13	12.280928157374042	24.604451723265147	50.87246091170695	12.242159207653865
14-15	13.407221986796221	22.38604743944288	51.01749322122367	13.189237352537234
16-17	16.28324829165241	22.976366708950767	51.939677533309535	8.800707466087287
18-19	16.320304811539053	19.76143380370385	51.39763015852707	12.520631226230023
20-21	13.369172537435722	19.370076794985977	54.23962722149756	13.021123446080749
22-23	13.394005650292648	20.157882726423175	55.30519083967894	11.142920783605238
24-25	13.459116069058172	20.799688077837057	54.021403013104894	11.71979283999988
26-27	15.89135286187724	20.39464196954705	52.055168632322236	11.658836536253471
28-29	14.342080759490628	22.409108279863048	53.70959254695319	9.539218413693135
30-31	14.441120966433713	21.333319231754203	53.62398231888426	10.601577482927825
32-33	14.808710965277486	21.976109965224012	53.310960100020786	9.904218969477716
34-35	14.79362757041086	22.07402873859767	53.32260893131987	9.809734759671596
36-37	14.991900876652775	21.420490612232378	53.109873972350066	10.47773453876478
38-39	15.363410574767114	21.47175766956842	52.61240842407188	10.552423331592596
40-41	15.42132238956357	21.983559641896594	52.56992661580053	10.025191352739308
42-43	15.380544651920378	21.974487307844417	52.70506264485482	9.93990539538039
44-45	15.621504926989054	21.080207627424493	52.77521872532469	10.523068720261763
46-47	15.696488106998594	20.756409227758414	53.09287823896716	10.454224426275836
48-49	15.105457103958575	20.753704950366792	53.45202181623904	10.688816129435601
50-51	14.969596089442474	20.76031327588321	53.550647430049004	10.71944320462531
52-53	14.838261064328547	21.053542924499425	53.53771619142271	10.570479819749313
54-55	14.911195998329841	21.264696041843933	53.37759418999637	10.446513769829856
56-57	14.736092929478414	21.226919505214386	53.22073684659089	10.81625071871631
58-59	14.418337174245652	21.382657411765056	53.49810026727829	10.700905146710998
60-61	14.141213867842001	21.183356520685205	53.564707636720996	11.1107219747518
62-63	14.017329869774825	21.0975652811369	53.561846433374996	11.323258415713278
64-65	14.066584109141447	21.122807904195117	53.49582501565065	11.314782971012777
66-67	13.89484201588657	20.92320279181728	53.817501229253104	11.364453963043047
68-69	13.782174749760594	20.639646147938706	54.118346353847016	11.45983274845368
70-71	13.630781915157792	20.560878998039403	54.45252660727662	11.355812479526184
72-73	13.417903715310823	20.353298462855836	54.94380152056952	11.284996301263819
74-75	13.442477868363579	20.081474724997264	55.24872232712348	11.227325079515687
76-77	13.719589722929632	20.11858791540857	55.10249387058342	11.059328491078373
78-79	13.858621320202099	20.273909123337333	54.92647236305457	10.940997193405993
80-81	13.558487636867952	20.17500107112791	55.22543028784327	11.041081004160866
82-83	13.482426181346268	20.120012745007774	55.366382175352136	11.031178898293819
84-85	13.541223177484785	20.177986755777386	55.19147809072411	11.089311976013711
86-87	13.677721923220929	20.310062698062445	54.94387281801607	11.06834256070055
88-89	13.707386164424836	20.694686331182474	54.66848173608485	10.929445768307836
90-91	13.705565299275268	20.92482657838674	54.53659123795007	10.833016884387915
92-93	13.612982828100984	20.984607368654977	54.37644418821452	11.025965615029511
94-95	13.580175018494058	21.24902460201845	54.21763721301658	10.953163166470915
96-97	13.423922455553553	21.70717491477918	54.293649566233945	10.575253063433316
98-99	13.281579264157486	22.197068032831222	54.16799642905723	10.353356273954057
100	13.211655439388784	22.639552939399977	53.93392360668108	10.21486801453016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	782372.0
1	1634578.0
2	2109906.5
3	1116792.0
4	307337.5
5	85267.5
6	48238.5
7	37140.5
8	33694.0
9	34795.5
10	39574.0
11	48815.0
12	64226.0
13	86287.0
14	115578.5
15	151303.0
16	191850.0
17	235893.5
18	280594.5
19	321279.5
20	354215.5
21	379556.5
22	399064.5
23	414620.5
24	425590.5
25	429463.0
26	426850.0
27	417166.5
28	398837.0
29	375822.0
30	349174.5
31	319938.0
32	290585.0
33	261597.5
34	234213.0
35	208370.5
36	184706.0
37	163070.0
38	142900.5
39	124789.0
40	109764.5
41	98224.0
42	90193.5
43	87716.0
44	94679.0
45	111327.5
46	135558.0
47	165964.5
48	204058.0
49	258974.5
50	332936.0
51	423217.5
52	517801.5
53	588643.0
54	611016.0
55	571047.5
56	475847.0
57	354602.0
58	238589.5
59	146821.5
60	84227.0
61	45068.0
62	22693.5
63	11049.0
64	5372.5
65	2928.5
66	1791.5
67	1290.5
68	995.5
69	759.0
70	610.0
71	477.0
72	353.5
73	279.0
74	213.0
75	151.0
76	113.0
77	79.5
78	51.5
79	33.0
80	24.5
81	22.0
82	16.0
83	11.0
84	5.0
85	3.5
86	4.0
87	4.5
88	4.5
89	4.0
90	3.0
91	2.0
92	2.5
93	2.0
94	2.0
95	2.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.061074090844197605
2	0.006469211442753552
3	0.0
4	0.0
5	4.3231007254677673E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.338102605501928E-4
18-19	0.0
20-21	9.932838571610465E-4
22-23	0.0
24-25	4.889221058564737E-5
26-27	0.0
28-29	7.719822724049584E-6
30-31	2.5732742413498615E-4
32-33	7.05077142129862E-4
34-35	2.264481332387878E-4
36-37	0.0
38-39	1.9042229385988975E-4
40-41	1.0807751813669418E-4
42-43	2.6504724685903573E-4
44-45	1.0550424389534432E-4
46-47	1.9042229385988975E-4
48-49	5.635470588556197E-4
50-51	1.0550424389534432E-4
52-53	1.801291968944903E-5
54-55	5.661203330969695E-5
56-57	1.132240666193939E-4
58-59	7.719822724049584E-6
60-61	2.8306016654848476E-5
62-63	0.0
64-65	3.087929089619834E-5
66-67	0.0
68-69	2.5732742413498615E-5
70-71	9.932838571610465E-4
72-73	0.001093641552573691
74-75	2.8306016654848476E-4
76-77	9.778442117129474E-5
78-79	9.521114692994488E-4
80-81	2.341679559628374E-4
82-83	7.462495299914598E-4
84-85	3.268058286514324E-4
86-87	3.756980392370798E-4
88-89	0.0010473226162293937
90-91	1.7240937417044072E-4
92-93	1.6468955144639114E-4
94-95	0.0031316747517227813
96-97	4.7862900889107424E-4
98-99	0.0017240937417044072
100	0.0014976456084656194
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9430498E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.19281063696834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.6701680427874	50.71058034193972
2	11.30860422152861	13.387761365067274
3	2.253759182708969	4.002190215702616
4	0.4765171427556925	1.1282555598562767
5	0.10836339856730282	0.3207167065686341
6	0.043567322328038983	0.15473233563140246
7	0.022274557816628314	0.0922945578003318
8	0.01474784738123078	0.06983732298720824
9	0.01163290884723942	0.061972611349658105
>10	0.06888597812433446	0.7782981939295629
>50	0.008416671027724157	0.357099582642715
>100	0.009754967957243725	1.252621604011259
>500	0.0012715140996397121	0.5149225085202458
>1k	8.47745410463943E-4	1.1456924203385308
>5k	3.7577531140775637E-4	1.6092590822571486
>10k+	8.127233479284033E-4	24.413765591397496
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	186737	0.9610510240138981	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	147712	0.7602069694765414	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	145369	0.748148606381576	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	144846	0.7454569615251241	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131342	0.6759579708147471	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	129393	0.6659273478219653	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	128844	0.6631018927049631	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123977	0.6380536412396635	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	118571	0.6102314001421889	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111027	0.5714058383887022	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	109920	0.5657086092183535	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106366	0.5474177759108387	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105291	0.5418852362919365	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104444	0.5375261097270899	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102293	0.5264558839408028	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97656	0.502591338626524	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97397	0.501258382569505	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	95165	0.48977128635611916	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93678	0.48211836876234465	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91428	0.4705386346762703	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87687	0.4512853968024906	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82369	0.42391605197149346	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82243	0.4232675868626733	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81573	0.4198193993792645	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77864	0.4007308510569312	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76377	0.39307793346315667	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73911	0.3803865449048192	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73569	0.3786264253237359	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71897	0.370021396260662	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68303	0.3515247010138392	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67076	0.34520988602556657	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63064	0.32456193351297535	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60725	0.31252415661194066	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58540	0.30127894817724177	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49749	0.2560356404658285	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49360	0.2540336331060583	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42976	0.2211780675925033	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41283	0.21246496101129264	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39373	0.2026350534093362	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38644	0.19888321956544808	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37012	0.19048405244168215	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35491	0.18265615219949588	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34708	0.178626404737542	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34437	0.17723169009873035	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34133	0.17566713935998965	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33962	0.17478707956944797	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33815	0.17403053694249113	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32841	0.1690177987203416	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28742	0.14792209648975543	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28324	0.14577083922398695	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28093	0.1445819865244833	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27951	0.14385117663993996	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27361	0.14081471303514712	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26260	0.13514836315569473	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25884	0.13321326092619962	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24328	0.12520523148711887	No Hit
TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24181	0.124448688860162	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24117	0.12411930975726922	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23073	0.1187463131413307	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22797	0.11732586576010558	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22723	0.11694502117238581	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22620	0.11641492667866774	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0021255245233549854	0.0	0.0	0.0	0.0
2	0.00520830706449212	0.0	0.0	3.087929089619834E-5	0.0
3	0.008486658447971843	0.0	0.0	5.146548482699723E-5	0.0
4	0.013885387806323852	0.0	0.0	6.175858179239668E-5	0.0
5	0.023663829923453325	0.0	0.0	6.175858179239668E-5	0.0
6	0.043678756972672546	0.0	0.0	6.175858179239668E-5	0.0
7	0.07346697959053855	0.0	0.0	6.175858179239668E-5	0.0
8	0.20300560490009056	0.0	0.0	6.175858179239668E-5	0.0
9	0.6636988923289563	0.0	0.0	6.175858179239668E-5	0.0
10-11	1.4961145102920161	0.0	0.0	7.205167875779612E-5	0.0
12-13	2.2449630472672393	0.0	0.0	7.462495299914598E-5	0.0
14-15	2.9318574336077234	0.0	0.0	7.719822724049584E-5	0.0
16-17	3.4729552479818064	0.0	0.0	7.97715014818457E-5	0.0
18-19	3.9841464691229223	0.0	0.0	1.0807751813669418E-4	0.0
20-21	4.625949370932233	0.0	0.0	1.466766317569421E-4	0.0
22-23	5.427511430741507	0.0	0.0	1.4924990599829197E-4	0.0
24-25	6.426224896551802	0.0	0.0	1.5182318023964183E-4	0.0
26-27	7.581331677654376	0.0	5.146548482699723E-6	1.595430029636914E-4	0.0
28-29	8.96262411802312	0.0	5.146548482699723E-6	1.595430029636914E-4	0.0
30-31	10.542575903098314	0.0	5.146548482699723E-6	1.595430029636914E-4	0.0
32-33	12.369194037126583	0.0	5.146548482699723E-6	1.6468955144639114E-4	0.0
34-35	14.180753370294472	0.0	5.146548482699723E-6	1.7240937417044072E-4	0.0
36-37	15.382300031630688	0.0	5.146548482699723E-6	1.878490196185399E-4	0.0
38-39	16.19863783213379	0.0	5.146548482699723E-6	2.213015847560881E-4	0.0
40-41	17.031573251493604	0.0	5.146548482699723E-6	2.341679559628374E-4	0.0
42-43	17.668098367833906	0.0	5.146548482699723E-6	2.521808756522864E-4	0.0
44-45	18.129980507962276	0.0	5.146548482699723E-6	2.6504724685903573E-4	0.0
46-47	18.495758574998952	0.0	5.146548482699723E-6	2.753403438244352E-4	0.0
48-49	18.808002244718587	0.0	5.146548482699723E-6	3.062196347206335E-4	0.0
50-51	19.119131686691716	0.0	5.146548482699723E-6	3.551118453062809E-4	0.0
52-53	19.449576125120416	0.0	5.146548482699723E-6	4.4517644375352604E-4	0.0
54-55	19.749684233517844	0.0	5.146548482699723E-6	5.120815740286224E-4	0.0
56-57	20.045466667915562	0.0	5.146548482699723E-6	5.455341391661706E-4	0.0
58-59	20.33410054647081	0.0	1.0293096965399446E-5	5.76413430062369E-4	0.0
60-61	20.622294395130787	0.0	1.0293096965399446E-5	6.072927209585673E-4	0.0
62-63	20.90732568974815	0.0	1.0293096965399446E-5	6.536116573028648E-4	0.0
64-65	21.19245476878668	0.0	1.0293096965399446E-5	6.767711254750136E-4	0.0
66-67	21.459179790451074	0.0	1.0293096965399446E-5	7.539693527155094E-4	0.0
68-69	21.70344784781121	0.0	1.0293096965399446E-5	8.002882890598069E-4	0.0
70-71	21.938861268506862	0.0	1.0293096965399446E-5	8.285943057146554E-4	0.0
72-73	22.170430217485936	0.0	1.0293096965399446E-5	8.72339967817603E-4	0.0
74-75	22.398486647125566	0.0	1.0293096965399446E-5	9.006459844724515E-4	0.0
76-77	22.62776538202984	0.0	1.0293096965399446E-5	0.001006150228367796	0.0
78-79	22.847646519404698	0.0	1.0293096965399446E-5	0.001191425973744986	0.0
80-81	23.067398993067496	0.0	1.0293096965399446E-5	0.0014230206554664733	0.0
82-83	23.29191459735103	0.0	1.0293096965399446E-5	0.0014616197690867213	0.0
84-85	23.522783615736458	0.0	1.0293096965399446E-5	0.0015156585281550683	0.0
86-87	23.754589305945736	0.0	1.0293096965399446E-5	0.0015362447220858672	0.0
88	23.932428288765426	0.0	1.0293096965399446E-5	0.001543964544809917	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	4190	0.0	62.07087	1
TGCCCTA	8000	0.0	56.457363	6
GTTACAC	1895	0.0	49.851017	7
CCCCTAC	50145	0.0	49.084244	7
TGCTCTA	7270	0.0	48.615036	6
CCCCCTA	28455	0.0	47.486095	6
GCTTTTC	3840	0.0	46.662766	1
CGCCCTA	8535	0.0	46.03519	6
GACTTTT	3835	0.0	45.987797	1
ATGCCCT	2885	0.0	43.333645	5
CCTTTTC	8365	0.0	43.178944	1
GCTTTCT	2695	0.0	43.103733	1
CATTTTT	32035	0.0	42.941566	1
GCCCCTA	6185	0.0	42.249565	6
CACTTTT	6790	0.0	41.766132	1
TTGCCCT	3875	0.0	41.723057	5
ACTTTTC	9130	0.0	41.106346	1
CTGCCCT	4250	0.0	40.916855	5
ACTTTTT	38340	0.0	40.884613	1
CTCCCTA	23880	0.0	40.228493	6
>>END_MODULE