FastQCFastQC Report
Thu 26 May 2016
SRR1248476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231558
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTGA25700.046.4513749
GCTTGCT4950.043.677767
CCTGCTG10900.042.6880848
ACTTGCT4000.041.125937
CTTGCTG16950.040.20658
TGCTTGC2700.038.2963076
GTTGCTG5850.037.7606938
CTGCTGA18300.037.754119
CATGCGC906.1861283E-736.5613061
CGTTGCC1850.035.5699733
GCCTGCT3850.035.4033977
TACCCGA21450.035.27746
ACTGCTG3000.034.4666758
GTAACGC1107.6972356E-834.1841282
TGCCCGA3350.033.673165
TACGATG851.7536295E-533.1816861
TATGCGC1001.4112084E-632.902222
ATTGCGC1151.13746864E-732.697862
ACCCGAC23900.032.6450927
GACACGC2750.032.4749222
Produced by FastQC (version 0.11.5)