##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248476_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2231558
Sequences flagged as poor quality	0
Sequence length	100
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.077375537628868	33.0	31.0	34.0	27.0	34.0
2	31.297655718560755	34.0	31.0	34.0	27.0	34.0
3	31.37481884853542	34.0	31.0	34.0	28.0	34.0
4	34.583117714170996	37.0	35.0	37.0	31.0	37.0
5	34.4405836639693	37.0	35.0	37.0	30.0	37.0
6	34.25726555169079	37.0	35.0	37.0	29.0	37.0
7	34.323650113508144	37.0	35.0	37.0	30.0	37.0
8	34.439811109547676	37.0	35.0	37.0	30.0	37.0
9	35.955613970150004	39.0	37.0	39.0	30.0	39.0
10-11	35.962292263969836	39.0	37.0	39.0	30.0	39.0
12-13	35.95319548046701	39.0	37.0	39.0	30.0	39.0
14-15	37.042017729317365	40.0	37.5	41.0	30.0	41.0
16-17	36.9014854196037	40.0	37.0	41.0	30.0	41.0
18-19	36.827525881021245	40.0	37.0	41.0	30.0	41.0
20-21	36.70407244624607	40.0	37.0	41.0	29.5	41.0
22-23	36.64901629265293	40.0	37.0	41.0	29.0	41.0
24-25	36.582289369131345	40.0	37.0	41.0	28.5	41.0
26-27	36.44535096107742	39.5	37.0	41.0	27.5	41.0
28-29	36.278733736698754	39.0	36.5	41.0	27.0	41.0
30-31	36.23682400367815	39.0	36.0	41.0	27.0	41.0
32-33	36.14882382622365	39.0	36.0	41.0	27.0	41.0
34-35	36.00407607599713	39.0	36.0	40.5	26.0	41.0
36-37	35.858394225021264	39.0	35.5	40.0	26.0	41.0
38-39	35.68751428374257	39.0	35.0	40.0	25.5	41.0
40-41	35.43760973275174	39.0	35.0	40.0	25.0	41.0
42-43	35.121092304121156	38.5	34.0	40.0	24.0	41.0
44-45	34.79727056164348	38.0	33.5	40.0	23.0	41.0
46-47	34.42250503908032	37.5	33.0	39.5	23.0	41.0
48-49	33.91205852592673	37.0	32.5	39.0	22.5	40.5
50-51	33.335958106399204	36.0	32.0	39.0	22.0	40.0
52-53	32.76146105097873	35.0	31.0	37.5	22.0	39.5
54-55	32.2278197564213	35.0	31.0	37.0	21.5	39.0
56-57	31.727333100909767	34.0	30.0	36.0	21.0	39.0
58-59	31.766655852099746	34.0	31.0	36.0	22.0	37.5
60-61	31.514078056676098	34.0	31.0	35.0	22.0	37.0
62-63	31.238062152092844	34.0	30.0	35.0	21.0	36.0
64-65	31.018491116968505	34.0	30.0	35.0	20.5	36.0
66-67	30.851013955272506	34.0	30.0	35.0	20.0	35.0
68-69	30.698013674751003	34.0	30.0	35.0	20.0	35.0
70-71	30.56229100924108	34.0	30.0	35.0	20.0	35.0
72-73	30.693187002085537	34.0	30.0	35.0	20.0	35.0
74-75	30.956767200314758	34.0	31.0	35.0	20.0	35.0
76-77	30.937127558414346	34.0	31.0	35.0	20.0	35.0
78-79	30.78215824997603	34.0	31.0	35.0	20.0	35.0
80-81	30.597080380613008	34.0	30.0	35.0	19.5	35.0
82-83	30.39330570838849	34.0	30.0	35.0	18.0	35.0
84-85	30.180487130515992	34.0	29.5	35.0	17.0	35.0
86-87	29.941211700524924	34.0	29.0	35.0	15.0	35.0
88-89	29.649868163856823	34.0	29.0	35.0	12.5	35.0
90-91	29.30618540947625	34.0	28.0	35.0	10.0	35.0
92-93	28.94058007902999	34.0	27.0	35.0	9.0	35.0
94-95	28.530605075019338	33.5	26.0	35.0	8.0	35.0
96-97	28.10016096377508	33.0	25.0	35.0	7.5	35.0
98-99	27.639497382546185	33.0	24.0	35.0	7.0	35.0
100	26.25115367828217	31.0	20.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2509503161472182
1101	2	0.3073935456005543
1101	3	0.32340511539975125
1101	4	0.2842745818797141
1101	5	0.2652526043175456
1101	6	0.1586671998943885
1101	7	0.1459013112702081
1101	8	0.1423876263089099
1101	9	0.19016379249915616
1101	10-11	0.35934536489240543
1101	12-13	0.3383837164930483
1101	14-15	0.5655694617201092
1101	16-17	0.5646446067648725
1101	18-19	0.525144379418542
1101	20-21	0.5056940540550912
1101	22-23	0.5446357180246011
1101	24-25	0.5340164355278105
1101	26-27	0.633501979451168
1101	28-29	0.5767890951369381
1101	30-31	0.5641600400843885
1101	32-33	0.5970965024069272
1101	34-35	0.5839740857931446
1101	36-37	0.5855877552430826
1101	38-39	0.5945478858717195
1101	40-41	0.6982992345535379
1101	42-43	0.6452194125322706
1101	44-45	0.658284481505504
1101	46-47	0.6261226271048059
1101	48-49	0.6111848513468701
1101	50-51	0.6019886717772991
1101	52-53	0.6144286479018959
1101	54-55	0.6297357310519018
1101	56-57	0.6005368812120935
1101	58-59	0.5746195501144769
1101	60-61	0.5936233403676283
1101	62-63	0.550016688377589
1101	64-65	0.487949514061949
1101	66-67	0.3676382333496413
1101	68-69	0.47384762328515606
1101	70-71	0.4258626715459748
1101	72-73	0.49246160110115156
1101	74-75	0.49017341546778326
1101	76-77	0.4506239834700665
1101	78-79	0.4619674032279235
1101	80-81	0.48001933080807646
1101	82-83	0.44734914341054477
1101	84-85	0.38981200395947013
1101	86-87	0.44064861182428317
1101	88-89	0.582191911124724
1101	90-91	0.5623219926560665
1101	92-93	0.47879345764933845
1101	94-95	0.4503656516158472
1101	96-97	0.5139105596537732
1101	98-99	0.6295222497400808
1101	100	0.5978198701500581
1102	1	0.2343232764500236
1102	2	0.2850972822201818
1102	3	0.34556576779598913
1102	4	0.26082521204589426
1102	5	0.18325151022370534
1102	6	0.07245661810674164
1102	7	0.10550894821045631
1102	8	0.15287912465685594
1102	9	0.2648056597054307
1102	10-11	0.3275585751018255
1102	12-13	0.45003540280796983
1102	14-15	0.5581279712503502
1102	16-17	0.5989292234159933
1102	18-19	0.6411276505230674
1102	20-21	0.6154481898065214
1102	22-23	0.634271922970683
1102	24-25	0.5640866074256508
1102	26-27	0.5856264630170358
1102	28-29	0.567356106024171
1102	30-31	0.6826652742080768
1102	32-33	0.6170729209668622
1102	34-35	0.6719192253078106
1102	36-37	0.5961341353208951
1102	38-39	0.6370429273369353
1102	40-41	0.6191179991480951
1102	42-43	0.6470640287821041
1102	44-45	0.6089361282819681
1102	46-47	0.6847150700676465
1102	48-49	0.7226243283062814
1102	50-51	0.6504720771355323
1102	52-53	0.6795553214258234
1102	54-55	0.60232620181322
1102	56-57	0.6934171865197456
1102	58-59	0.5563357903264929
1102	60-61	0.5498222317566785
1102	62-63	0.6167917022012936
1102	64-65	0.5637186401972762
1102	66-67	0.600344675697567
1102	68-69	0.6121212565087895
1102	70-71	0.5129965056603432
1102	72-73	0.546107665874576
1102	74-75	0.45775914456566014
1102	76-77	0.5403501236380066
1102	78-79	0.48822748479516065
1102	80-81	0.4252047572892792
1102	82-83	0.4111999642464781
1102	84-85	0.38564506435946555
1102	86-87	0.5126856032904712
1102	88-89	0.4571024037443081
1102	90-91	0.5854307416889988
1102	92-93	0.6077488222794187
1102	94-95	0.5615118358898101
1102	96-97	0.5918825676110302
1102	98-99	0.5438184682708673
1102	100	0.48938206973897636
1103	1	0.07934051162006028
1103	2	0.01594394922983966
1103	3	0.13356803151981467
1103	4	0.014943645960599383
1103	5	-0.08819984543817583
1103	6	-0.228347689828297
1103	7	-0.12950674351152003
1103	8	-0.08930159596189924
1103	9	0.0032735302615876094
1103	10-11	0.02791185321401457
1103	12-13	0.18386092457915737
1103	14-15	0.2615630006616101
1103	16-17	0.38054504204369266
1103	18-19	0.3382928509140797
1103	20-21	0.32854005926511576
1103	22-23	0.36586458987744663
1103	24-25	0.415281064732163
1103	26-27	0.3388153982093698
1103	28-29	0.3567337534663366
1103	30-31	0.37052348277935465
1103	32-33	0.425261770412078
1103	34-35	0.40156028788606335
1103	36-37	0.42337427869006916
1103	38-39	0.41454288446337273
1103	40-41	0.3907922973079607
1103	42-43	0.354441710523119
1103	44-45	0.4077356686774323
1103	46-47	0.32618065975054833
1103	48-49	0.41052712537716474
1103	50-51	0.371229738640217
1103	52-53	0.43327760367102996
1103	54-55	0.47066665215707104
1103	56-57	0.451495422187115
1103	58-59	0.3647870259424124
1103	60-61	0.3309544366579864
1103	62-63	0.34242494464268347
1103	64-65	0.37876897375355156
1103	66-67	0.3951981338786972
1103	68-69	0.3458764656460005
1103	70-71	0.4659933673159493
1103	72-73	0.4273016086983965
1103	74-75	0.4147209528008169
1103	76-77	0.3871560436187984
1103	78-79	0.3194823080703557
1103	80-81	0.3015728525028756
1103	82-83	0.39375728619259576
1103	84-85	0.3335656968301848
1103	86-87	0.380026242277971
1103	88-89	0.2845637746691807
1103	90-91	0.2604063037613322
1103	92-93	0.2913522590237747
1103	94-95	0.2649177112821022
1103	96-97	0.3299096979980938
1103	98-99	0.2888271287294444
1103	100	0.23920971803861235
1104	1	0.12065194536220147
1104	2	0.10505912608002888
1104	3	0.19362225302722536
1104	4	0.1445150961684547
1104	5	0.13886266476036013
1104	6	-0.13283284513033777
1104	7	-0.0782137774087559
1104	8	0.017269566656253232
1104	9	0.09423228360493141
1104	10-11	0.1937116893382722
1104	12-13	0.1214000286051018
1104	14-15	0.25961269620415095
1104	16-17	0.43549230989520993
1104	18-19	0.34534491657830557
1104	20-21	0.3771601479151343
1104	22-23	0.38971469117132784
1104	24-25	0.37182268180065137
1104	26-27	0.3538810580138758
1104	28-29	0.41634785064697866
1104	30-31	0.37527781258788906
1104	32-33	0.42977020433754376
1104	34-35	0.4205784296863939
1104	36-37	0.5463809822961352
1104	38-39	0.4933245734406029
1104	40-41	0.5374806817786961
1104	42-43	0.5208540500031802
1104	44-45	0.5816290935048016
1104	46-47	0.5374603352399561
1104	48-49	0.5766001048898133
1104	50-51	0.608053024995769
1104	52-53	0.5803811270202388
1104	54-55	0.6481375716143631
1104	56-57	0.5639763116608449
1104	58-59	0.46607289227067383
1104	60-61	0.420204720006339
1104	62-63	0.4121299240780161
1104	64-65	0.39840049288628876
1104	66-67	0.40079759904615386
1104	68-69	0.442454477155966
1104	70-71	0.46574289590840223
1104	72-73	0.42009880777823483
1104	74-75	0.32118186070208665
1104	76-77	0.3826729550143284
1104	78-79	0.49352353776158253
1104	80-81	0.44132998571983606
1104	82-83	0.44981733187998074
1104	84-85	0.35034270561138925
1104	86-87	0.449407593324441
1104	88-89	0.5992437229009759
1104	90-91	0.6392535615466919
1104	92-93	0.5581740205472343
1104	94-95	0.5543929459216841
1104	96-97	0.7449968576266279
1104	98-99	0.6045648970929527
1104	100	0.6540354044366872
1105	1	0.1711156089446142
1105	2	0.13937219554736302
1105	3	0.1448647839507906
1105	4	0.10943456818098696
1105	5	0.09214891324099739
1105	6	-0.06442307705066952
1105	7	-0.013085085617326797
1105	8	0.11015565872282451
1105	9	0.0772743629946504
1105	10-11	0.1074781785440635
1105	12-13	0.12051775612277282
1105	14-15	0.18992037923711536
1105	16-17	0.2656556069922331
1105	18-19	0.31174521291735147
1105	20-21	0.27190956462826676
1105	22-23	0.1757926684792892
1105	24-25	0.24812324143283604
1105	26-27	0.36064511478935657
1105	28-29	0.31929149097436493
1105	30-31	0.226214260035853
1105	32-33	0.2949739679131014
1105	34-35	0.2984310341054126
1105	36-37	0.21969805297924694
1105	38-39	0.2565010601604598
1105	40-41	0.25427053758532026
1105	42-43	0.2527650933258272
1105	44-45	0.3090454097058455
1105	46-47	0.4148243150470208
1105	48-49	0.34312685625270234
1105	50-51	0.37724217328131715
1105	52-53	0.38906676213401425
1105	54-55	0.4743030397742203
1105	56-57	0.37918868265480654
1105	58-59	0.3455634221267161
1105	60-61	0.28428211651918645
1105	62-63	0.25986087020989856
1105	64-65	0.23828678593777397
1105	66-67	0.3085038173568009
1105	68-69	0.29675870406125426
1105	70-71	0.3831831899869691
1105	72-73	0.4205658533648453
1105	74-75	0.39369159521330843
1105	76-77	0.3181289773961353
1105	78-79	0.3190439091840247
1105	80-81	0.39528934496406265
1105	82-83	0.3546024952646505
1105	84-85	0.35907301801664815
1105	86-87	0.2963370189769101
1105	88-89	0.23081457240527214
1105	90-91	0.3684678073031442
1105	92-93	0.3542874542259362
1105	94-95	0.30569240483004023
1105	96-97	0.35908158423440995
1105	98-99	0.438265993142938
1105	100	0.5643488141365474
1106	1	0.13629369862633212
1106	2	0.060956284564753105
1106	3	0.12507088010718803
1106	4	0.13313041359612043
1106	5	0.11697710637005088
1106	6	0.12344239205570062
1106	7	0.048179528294802765
1106	8	0.17205548532168535
1106	9	0.10532242732885777
1106	10-11	0.09107189085343492
1106	12-13	0.06166922398811181
1106	14-15	0.16553844312215205
1106	16-17	0.18747495483314935
1106	18-19	0.1951603946946463
1106	20-21	0.2272823382646436
1106	22-23	0.2721384349017981
1106	24-25	0.1988835835327194
1106	26-27	0.20317503022926786
1106	28-29	0.28432221463993557
1106	30-31	0.3040151986312196
1106	32-33	0.28273621149207173
1106	34-35	0.17661409846457587
1106	36-37	0.19329818673157462
1106	38-39	0.16659572930919353
1106	40-41	0.08421459117942476
1106	42-43	0.174040309827717
1106	44-45	0.22936766854544288
1106	46-47	0.2581947574445209
1106	48-49	0.2519542885683066
1106	50-51	0.1745743909027908
1106	52-53	0.23290869260387126
1106	54-55	0.19558205479807356
1106	56-57	0.20235597898887292
1106	58-59	0.09703763681694966
1106	60-61	0.12359368570801621
1106	62-63	0.11664397682415029
1106	64-65	0.12024383435866781
1106	66-67	0.08579467267858476
1106	68-69	0.12291948523699858
1106	70-71	0.17924941846501596
1106	72-73	0.05632031468729792
1106	74-75	0.09226640086484039
1106	76-77	0.10365236658714849
1106	78-79	0.09930939536987893
1106	80-81	0.09026844754800223
1106	82-83	0.10124648725060226
1106	84-85	0.053640798897937714
1106	86-87	0.04572246248623202
1106	88-89	0.025604968854512578
1106	90-91	0.13518379930240698
1106	92-93	0.09112918007468451
1106	94-95	0.2315283430621946
1106	96-97	0.1968487908457206
1106	98-99	0.15987647772897517
1106	100	0.10839919196471115
1107	1	-0.014918778500675245
1107	2	-0.012052032121040668
1107	3	0.058932666341121376
1107	4	-0.019050158541219275
1107	5	-0.020458578888948864
1107	6	-0.06582323129938317
1107	7	-0.038998431707703674
1107	8	-0.017550890260082497
1107	9	0.007607063402794267
1107	10-11	-0.03073573155866427
1107	12-13	0.08270036174724282
1107	14-15	0.17712173220252936
1107	16-17	0.011599324732955552
1107	18-19	0.09380494510705972
1107	20-21	0.07561087685545687
1107	22-23	0.05368263073461321
1107	24-25	0.002808206270685787
1107	26-27	0.17530149808673912
1107	28-29	0.11329961362079644
1107	30-31	0.09098587367393662
1107	32-33	0.1815601051816813
1107	34-35	0.194914645156679
1107	36-37	0.18806338064919004
1107	38-39	0.10623725617329427
1107	40-41	0.10506127437795243
1107	42-43	0.13703416965194037
1107	44-45	0.0840936956847429
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2216	74-75	-0.9391077323545396
2216	76-77	-0.8982724500105128
2216	78-79	-1.0846242581216288
2216	80-81	-1.271931003259045
2216	82-83	-1.2308631419262426
2216	84-85	-1.2260724076738896
2216	86-87	-1.2682714399815467
2216	88-89	-1.2076600690994184
2216	90-91	-1.2442005706774353
2216	92-93	-1.3770828110469182
2216	94-95	-1.5267086079453023
2216	96-97	-1.3792854642865748
2216	98-99	-1.4748248384663647
2216	100	-1.4322412753115188
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	2234.0
1	109.0
2	75.0
3	73.0
4	49.0
5	277.0
6	1383.0
7	2651.0
8	5172.0
9	8440.0
10	11206.0
11	14170.0
12	14847.0
13	14276.0
14	13855.0
15	13558.0
16	12753.0
17	12409.0
18	12148.0
19	12339.0
20	13275.0
21	13961.0
22	15383.0
23	16765.0
24	19354.0
25	21784.0
26	25635.0
27	29952.0
28	37123.0
29	49121.0
30	66752.0
31	84234.0
32	106793.0
33	138300.0
34	189807.0
35	320348.0
36	593034.0
37	307495.0
38	26620.0
39	3754.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.190478733261926	22.259456582756233	26.906247938660826	31.64381674532101
2	18.171759321577603	20.076681473504735	30.646969085116883	31.10459011980078
3	16.864242511262272	21.323339375774	27.388184934580114	34.424233178383616
4	16.25108468288673	18.544644964334463	27.974139903677468	37.23013044910134
5	16.2101324264117	19.67085205616513	28.736060230125382	35.38295528729779
6	15.878801527429856	16.013295027957422	29.432004967018138	38.675898477594586
7	22.83226867804941	18.409975204650237	24.910197048335007	33.84755906896535
8	18.767748656149145	18.54922428558995	22.570682831458537	40.112344226802364
9	15.006399590139113	16.339658751332966	24.431001741101248	44.222939917426665
10-11	24.68132278298725	25.231891678009276	22.99953612691998	27.08724941208349
12-13	24.16727998203683	27.636988782665632	20.455355538602305	27.74037569669523
14-15	26.715041649505256	26.497618938657723	19.201171193240604	27.58616821859642
16-17	26.96403583899103	26.068150790791616	19.81783954823957	27.149973821977785
18-19	28.208790020562425	22.796022833576288	21.622847574706473	27.372339571154818
20-21	25.557887916984384	22.20422288282116	24.183955791169677	28.05393340902478
22-23	25.686110127139152	23.599249544088835	25.5015695817238	25.213070747048217
24-25	25.675262163243843	24.085075853701873	24.064167403996116	26.175494579058167
26-27	28.61670388117441	24.852725445772073	21.50716625114891	25.023404421904612
28-29	27.46984054197512	26.347298941059442	23.126842691623104	23.056017825342334
30-31	27.824412804713628	25.519198231493295	23.05546594471487	23.60092301907821
32-33	28.773718556878887	26.0883766872842	21.905614607900826	23.232290147936087
34-35	28.86850866408823	26.20974182722076	21.55628292218922	23.36546658650179
36-37	29.700605143110714	25.768431244893392	21.099312657572995	23.431650954422903
38-39	30.33162607152552	25.953116481918954	20.8133161934119	22.90194125314363
40-41	30.942916566742863	26.1271910234359	20.661379323186235	22.268513086635007
42-43	31.21258116387835	26.402286587007318	20.942995050304557	21.442137198809778
44-45	31.53750242840523	25.946270440666375	20.979132988668038	21.53709414226035
46-47	31.85092643233928	25.086245226732185	21.43671154992846	21.626116791000076
48-49	31.719158747781595	25.24680920846744	21.936996042483017	21.09703600126795
50-51	32.025672403176074	25.10924343356054	22.2727389991038	20.592345164159585
52-53	32.08601280222682	25.27304672269903	22.468886156016314	20.17205431905784
54-55	32.02476520702881	25.644974003621723	22.459273537176525	19.870987252172945
56-57	31.95920229750665	25.825055151735132	21.7832865453994	20.432456005358816
58-59	31.582020738744003	25.7058712814182	22.044320692060182	20.66778728777761
60-61	31.440426652165556	25.255244483060235	22.594366595933362	20.709962268840844
62-63	31.503297590739827	24.53696890843017	22.91349993270223	21.046233568127775
64-65	31.25394223410164	24.39833544632595	22.98269768593662	21.365024633635795
66-67	30.765648699827864	24.11371476022756	23.449306453733	21.671330086211576
68-69	30.570665034917667	24.141570974128136	23.085270698815354	22.20249329213885
70-71	30.15075816472144	24.208111404747946	23.211961780656427	22.42916864987419
72-73	30.124357352426586	24.6789453850502	23.17813338357873	22.018563878944487
74-75	29.893197482930283	25.33974003379511	23.00102219786673	21.766040285407872
76-77	29.10792656584566	26.294480415629813	23.068109581494586	21.52948343702994
78-79	28.463612343496962	27.23305326149857	22.779440065674077	21.523894329330385
80-81	27.83412821581985	28.507701683440207	22.356381149722395	21.30178895101755
82-83	27.260231724317368	29.75810367818335	22.236001718137906	20.745662879361372
84-85	26.648053539058637	31.034414698030865	22.11103639742676	20.206495365483743
86-87	26.112060391903185	32.19072723382169	21.819033336578183	19.87817903769694
88-89	25.84604997986944	32.996874435755466	21.56715589354465	19.589919690830445
90-91	25.834189067153684	33.81720987258875	21.080570872032016	19.268030188225552
92-93	25.95825200514818	34.387388482628026	20.50679253899603	19.147566973227757
94-95	26.223314831990685	34.46538820856236	20.02972028168098	19.281576677765976
96-97	26.51581136410181	34.23382340109253	19.928036462243593	19.322328772562066
98-99	26.90780520161338	33.74664617001083	19.753371663246195	19.59217696512959
100	27.677942932104177	33.15108084087684	19.328260412147554	19.842715814871426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3734.0
1	2582.0
2	1091.0
3	667.5
4	548.5
5	505.0
6	467.5
7	448.0
8	451.5
9	422.0
10	410.0
11	436.5
12	481.5
13	535.5
14	603.0
15	650.0
16	688.5
17	774.0
18	877.5
19	981.0
20	1070.5
21	1174.5
22	1328.0
23	1496.5
24	1714.5
25	1981.0
26	2157.5
27	2409.0
28	2690.0
29	2943.5
30	3358.0
31	3778.5
32	4210.0
33	4768.0
34	5445.5
35	6302.5
36	7356.5
37	8627.5
38	10104.5
39	11929.0
40	14477.5
41	17909.0
42	22735.0
43	29773.5
44	39118.5
45	50193.5
46	62663.5
47	75518.5
48	88846.5
49	104396.5
50	124243.5
51	150166.0
52	179154.0
53	202538.5
54	211866.5
55	201797.0
56	172570.5
57	131845.0
58	90139.5
59	55473.5
60	31270.5
61	17020.5
62	9486.0
63	5714.5
64	4041.0
65	3212.0
66	2684.5
67	2319.5
68	1972.5
69	1736.0
70	1541.0
71	1401.5
72	1289.0
73	1162.5
74	1076.0
75	1020.0
76	1021.0
77	968.0
78	887.5
79	883.0
80	881.5
81	838.0
82	815.5
83	796.0
84	815.5
85	827.0
86	818.5
87	808.5
88	769.5
89	733.0
90	731.5
91	747.5
92	759.0
93	738.5
94	702.0
95	673.5
96	618.5
97	577.0
98	515.0
99	408.0
100	347.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1358243881628889
2	0.1280719569018596
3	0.12896819172972424
4	0.12529362893547916
5	0.12426295888343482
6	0.1099680133789935
7	0.11252228263840779
8	0.11274634134537395
9	0.11243265915562133
10-11	0.11202935348308223
12-13	0.11438196990622695
14-15	0.12188793658959346
16-17	0.11601759846707996
18-19	0.12256011271049194
20-21	0.12424055301273819
22-23	0.12455423520249083
24-25	0.1252040054526927
26-27	0.1260330226684675
28-29	0.12641392247030997
30-31	0.1248006997801536
32-33	0.1250919760992096
34-35	0.12627948724613028
36-37	0.12677241640145584
38-39	0.12739978078096112
40-41	0.12441979997831112
42-43	0.12251530096909873
44-45	0.12229124226213256
46-47	0.12273935967606489
48-49	0.12374762385741263
50-51	0.12123816633939158
52-53	0.1222016187793461
54-55	0.12484551152154684
56-57	0.12130538395148144
58-59	0.12253770683979533
60-61	0.1238372473401991
62-63	0.11910960862321301
64-65	0.11167085955193636
66-67	0.11234303567283487
68-69	0.11010244860317321
70-71	0.10866847287858976
72-73	0.11193973000029576
74-75	0.11370979378532844
76-77	0.11019207208595967
78-79	0.10611420361917548
80-81	0.10766020869724202
82-83	0.10757058521445556
84-85	0.10338068739418828
86-87	0.10687600322286044
88-89	0.10577811555872622
90-91	0.11099868343103787
92-93	0.10848922591301684
94-95	0.10936305487018487
96-97	0.11258950025049763
98-99	0.11545745169966454
100	0.11646571588101227
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2231558.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.14944807583366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.48832711963644	96.66574948704478
2	1.4739293134901512	2.8933069724370153
3	0.027976943493946745	0.08237764688338976
4	0.002172941553264527	0.00853092056615832
5	8.886999690283471E-4	0.004361270573257137
6	0.0013248987105120278	0.007802284631588355
7	9.949092704946974E-4	0.00683548570492094
8	2.965435140786341E-4	0.0023284465789828925
9	1.3466289914412262E-4	0.0011895380304558561
>10	0.003012411194869843	0.05593351300095011
>50	5.762523439396389E-4	0.03898341997547503
>100	2.7398039979647723E-4	0.07775908270055304
>500	0.0	0.0
>1k	9.132352432931058E-5	0.15484193187249762
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.005556655932760879	0.0	0.0	0.0	0.0
2	0.012278417141745811	0.0	0.0	0.0	0.0
3	0.01908980183351721	0.0	0.0	0.0	0.0
4	0.031099348526903624	0.0	0.0	0.0	0.0
5	0.04965140946370204	0.0	0.0	0.0	0.0
6	0.0840668268537049	0.0	0.0	0.0	0.0
7	0.12668279291866938	0.0	0.0	0.0	0.0
8	0.2798045132593462	0.0	0.0	0.0	0.0
9	0.857024554145579	0.0	0.0	0.0	0.0
10-11	2.102634123782577	0.0	0.0	0.0	0.0
12-13	3.6995677459425207	0.0	0.0	0.0	0.0
14-15	5.401786554505866	0.0	0.0	0.0	0.0
16-17	7.128226109292252	0.0	0.0	0.0	0.0
18-19	8.925042503936712	0.0	0.0	0.0	0.0
20-21	11.137891105675944	0.0	0.0	0.0	0.0
22-23	13.646609229963998	0.0	0.0	0.0	0.0
24-25	16.367130049947168	0.0	0.0	0.0	0.0
26-27	19.194280408575533	0.0	0.0	0.0	0.0
28-29	22.257543832604846	0.0	0.0	0.0	0.0
30-31	25.574957047945873	0.0	0.0	0.0	0.0
32-33	29.25382176936472	0.0	0.0	0.0	0.0
34-35	32.677349188324925	0.0	0.0	0.0	0.0
36-37	35.500444084357206	0.0	0.0	0.0	0.0
38-39	37.769083304131016	0.0	0.0	0.0	0.0
40-41	39.87743092494123	0.0	0.0	0.0	0.0
42-43	41.70451765089682	0.0	0.0	0.0	0.0
44-45	43.27989682544661	0.0	0.0	0.0	0.0
46-47	44.68909165703961	0.0	0.0	0.0	0.0
48-49	46.0122255392869	0.0	0.0	0.0	0.0
50-51	47.318129306968494	0.0	0.0	0.0	0.0
52-53	48.639605154784235	0.0	0.0	0.0	0.0
54-55	49.984988066633264	0.0	0.0	0.0	0.0
56-57	51.37083598096039	0.0	0.0	0.0	0.0
58-59	52.77191988736121	0.0	0.0	0.0	0.0
60-61	54.166909396932546	0.0	0.0	0.0	0.0
62-63	55.529522423347274	0.0	0.0	0.0	0.0
64-65	56.839974582780286	0.0	0.0	0.0	0.0
66-67	58.05253549313977	0.0	0.0	0.0	0.0
68-69	59.173613233444975	0.0	0.0	0.0	0.0
70-71	60.237063074318485	0.0	0.0	0.0	0.0
72-73	61.26690410914706	0.0	0.0	0.0	0.0
74-75	62.254510077712524	0.0	0.0	0.0	0.0
76-77	63.206692364706626	0.0	0.0	0.0	0.0
78-79	64.13187557751132	0.0	0.0	0.0	0.0
80-81	65.02557854198726	0.0	0.0	0.0	0.0
82-83	65.89467986043832	0.0	0.0	0.0	0.0
84-85	66.74617016452183	0.0	0.0	0.0	0.0
86-87	67.58148342996239	0.0	0.0	0.0	0.0
88	68.20109537820662	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	2570	0.0	46.451374	9
GCTTGCT	495	0.0	43.67776	7
CCTGCTG	1090	0.0	42.688084	8
ACTTGCT	400	0.0	41.12593	7
CTTGCTG	1695	0.0	40.2065	8
TGCTTGC	270	0.0	38.296307	6
GTTGCTG	585	0.0	37.760693	8
CTGCTGA	1830	0.0	37.75411	9
CATGCGC	90	6.1861283E-7	36.561306	1
CGTTGCC	185	0.0	35.569973	3
GCCTGCT	385	0.0	35.403397	7
TACCCGA	2145	0.0	35.2774	6
ACTGCTG	300	0.0	34.466675	8
GTAACGC	110	7.6972356E-8	34.184128	2
TGCCCGA	335	0.0	33.67316	5
TACGATG	85	1.7536295E-5	33.181686	1
TATGCGC	100	1.4112084E-6	32.90222	2
ATTGCGC	115	1.13746864E-7	32.69786	2
ACCCGAC	2390	0.0	32.645092	7
GACACGC	275	0.0	32.474922	2
>>END_MODULE