FastQCFastQC Report
Thu 26 May 2016
SRR1248476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2231558
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTT903.6379788E-1252.224183
TGCCCTA24750.046.9131136
CCACGTT1103.8198777E-1142.728873
CCGCCTA12050.041.7416156
CCCCCTA77500.041.7310456
CCACCTA13100.040.5489586
CCCCTAC160500.040.268117
GCCCCTA19000.040.0805746
CACCCTA33350.039.32616
TTGCCCT11300.039.101445
GTCCCTA10950.039.066086
GCCCTAC72750.038.9599047
CCCGCTA9250.038.1146626
ACCCGTG1850.038.1095354
CGCCCTA24700.037.6825946
CTCCCTA58150.037.6711356
CATGCTC2500.037.6014064
ATTCGCG1003.328205E-837.6014064
TCCCCCT33400.037.153595
ACCCCTA24000.036.8229946
Produced by FastQC (version 0.11.5)