##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248476_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2231558 Sequences flagged as poor quality 0 Sequence length 100 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.834853496973864 33.0 31.0 34.0 30.0 34.0 2 32.37055680381151 34.0 31.0 34.0 30.0 34.0 3 32.585709177175765 34.0 31.0 34.0 30.0 34.0 4 36.13039992686724 37.0 35.0 37.0 35.0 37.0 5 36.03098731917342 37.0 35.0 37.0 35.0 37.0 6 35.901606411305465 37.0 35.0 37.0 35.0 37.0 7 35.939535965455526 37.0 35.0 37.0 35.0 37.0 8 35.23344811114029 37.0 35.0 37.0 33.0 37.0 9 37.043412718826936 39.0 37.0 39.0 33.0 39.0 10-11 37.46162703366885 39.0 37.0 39.0 34.5 39.0 12-13 37.74006590910924 39.0 37.0 39.0 35.0 39.0 14-15 39.217816879507495 41.0 39.0 41.0 36.0 41.0 16-17 39.13125471083431 40.5 39.0 41.0 36.0 41.0 18-19 39.05504674312745 40.5 39.0 41.0 35.5 41.0 20-21 38.94507268016336 40.0 38.5 41.0 35.0 41.0 22-23 38.98754076748173 40.0 38.5 41.0 35.0 41.0 24-25 38.977069159752965 41.0 38.0 41.0 35.0 41.0 26-27 38.841235585183085 40.5 38.0 41.0 35.0 41.0 28-29 38.67093954089475 40.0 38.0 41.0 34.0 41.0 30-31 38.63450333802662 40.0 38.0 41.0 34.0 41.0 32-33 38.56170554383978 40.0 38.0 41.0 34.0 41.0 34-35 38.47031535814888 40.0 38.0 41.0 33.0 41.0 36-37 38.377533095711605 40.0 38.0 41.0 33.0 41.0 38-39 38.22163192711102 40.0 38.0 41.0 33.0 41.0 40-41 38.000662989713916 40.0 38.0 41.0 32.5 41.0 42-43 37.713639304916114 40.0 37.0 41.0 32.0 41.0 44-45 37.39353088738899 39.5 36.5 41.0 32.0 41.0 46-47 36.99610563561422 39.0 36.0 41.0 31.0 41.0 48-49 36.51699350857114 38.5 35.0 40.0 31.0 41.0 50-51 35.897079529189924 37.0 35.0 39.5 30.0 41.0 52-53 35.237655261480995 36.5 35.0 39.0 29.5 41.0 54-55 34.69887159553998 35.5 34.0 37.5 29.0 39.5 56-57 34.20111823219473 35.0 34.0 37.0 29.0 39.0 58-59 33.755449555870825 35.0 34.0 36.0 29.0 38.0 60-61 33.383447349340685 35.0 33.0 35.0 28.0 37.0 62-63 33.085900523311516 35.0 33.0 35.0 27.5 36.5 64-65 32.87587147634075 35.0 33.0 35.0 27.0 36.0 66-67 32.70762870604304 35.0 33.0 35.0 26.5 35.5 68-69 32.591320279374315 35.0 33.0 35.0 26.0 35.0 70-71 32.59527065843684 35.0 33.0 35.0 26.0 35.0 72-73 32.60684665153225 35.0 33.0 35.0 26.0 35.0 74-75 32.556438371756414 35.0 33.0 35.0 26.5 35.0 76-77 32.49459099875513 35.0 33.0 35.0 27.0 35.0 78-79 32.424658467312966 35.0 33.0 35.0 27.0 35.0 80-81 32.36221397785762 35.0 33.0 35.0 26.0 35.0 82-83 32.30387469203131 35.0 33.0 35.0 25.0 35.0 84-85 32.23874754767745 35.0 33.0 35.0 25.0 35.0 86-87 32.17884276366557 35.0 33.0 35.0 25.0 35.0 88-89 32.09913970418873 35.0 33.0 35.0 25.0 35.0 90-91 32.02092730728935 35.0 33.0 35.0 25.0 35.0 92-93 31.879776819603165 35.0 33.0 35.0 24.0 35.0 94-95 31.659706133562292 35.0 32.5 35.0 23.5 35.0 96-97 31.458106623265003 35.0 32.0 35.0 22.5 35.0 98-99 31.218390021679923 35.0 32.0 35.0 20.0 35.0 100 30.29450769372788 34.0 30.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18632988444556986 1101 2 0.2132952092668674 1101 3 0.26397094020926914 1101 4 0.13875515080263057 1101 5 0.17786052661494978 1101 6 -0.004930904366844402 1101 7 0.07522566002156594 1101 8 -0.17920650588109766 1101 9 -0.2646472040728085 1101 10-11 -0.015211338339717884 1101 12-13 0.1409712646217045 1101 14-15 0.25101516539906044 1101 16-17 0.32066937342712976 1101 18-19 0.4119390836109247 1101 20-21 0.4605302578267825 1101 22-23 0.398857489473329 1101 24-25 0.35422508871366887 1101 26-27 0.4298528092611207 1101 28-29 0.4878301800183067 1101 30-31 0.5027267473305983 1101 32-33 0.49682780411264105 1101 34-35 0.46064514133450984 1101 36-37 0.525423775249493 1101 38-39 0.5292856545053439 1101 40-41 0.49494305101548974 1101 42-43 0.6023305315189873 1101 44-45 0.6011449765176948 1101 46-47 0.6962726192568525 1101 48-49 0.6005049765850146 1101 50-51 0.5764920843052224 1101 52-53 0.5208184195295686 1101 54-55 0.5610392063188243 1101 56-57 0.5430659668951847 1101 58-59 0.5043584388453155 1101 60-61 0.5266982296939489 1101 62-63 0.6047841441947597 1101 64-65 0.6569971297499464 1101 66-67 0.6429079149201513 1101 68-69 0.6421166796682911 1101 70-71 0.6441016785905802 1101 72-73 0.5734727060115503 1101 74-75 0.5731630412450883 1101 76-77 0.5306313379215197 1101 78-79 0.6347852223279702 1101 80-81 0.688922139402905 1101 82-83 0.6447046652580823 1101 84-85 0.6333312581288979 1101 86-87 0.6554423494289594 1101 88-89 0.6981450953238308 1101 90-91 0.7129218147781842 1101 92-93 0.8560832768657249 1101 94-95 0.8370680947915403 1101 96-97 0.7407750157689144 1101 98-99 0.9102421405022021 1101 100 0.7402043477082003 1102 1 0.10377395376650611 1102 2 0.18404785022882209 1102 3 0.16862898277270233 1102 4 0.1115412483752678 1102 5 0.11804711269399348 1102 6 -0.05971349304952156 1102 7 0.07937755140531522 1102 8 -0.15720802014474344 1102 9 -0.016210241640827405 1102 10-11 0.14814100295618005 1102 12-13 0.17639765730461932 1102 14-15 0.32728094168342636 1102 16-17 0.3400726614918881 1102 18-19 0.4125086143367298 1102 20-21 0.39236537894721835 1102 22-23 0.4019801418778286 1102 24-25 0.3357437546981501 1102 26-27 0.35538780285530436 1102 28-29 0.36085268211768096 1102 30-31 0.3064286675420078 1102 32-33 0.3612208328346682 1102 34-35 0.338800088391352 1102 36-37 0.40955339527317847 1102 38-39 0.38510957975289273 1102 40-41 0.407643804547547 1102 42-43 0.436966037165611 1102 44-45 0.4661164468700534 1102 46-47 0.48262961133676896 1102 48-49 0.47953425451258624 1102 50-51 0.4353996546202197 1102 52-53 0.36840589540078383 1102 54-55 0.34803028892290655 1102 56-57 0.36022589048565123 1102 58-59 0.3536918618778202 1102 60-61 0.3892939702815923 1102 62-63 0.3843274219795205 1102 64-65 0.36203344316120933 1102 66-67 0.44640128455804273 1102 68-69 0.3869895163396677 1102 70-71 0.3699018158539147 1102 72-73 0.33440446795702883 1102 74-75 0.4281024854524915 1102 76-77 0.4297173505750038 1102 78-79 0.3737341958920908 1102 80-81 0.3589984368099479 1102 82-83 0.4187058915401778 1102 84-85 0.3855843301779913 1102 86-87 0.4290091838684873 1102 88-89 0.46788507985072414 1102 90-91 0.44511461134459296 1102 92-93 0.5024359728274348 1102 94-95 0.5096222137833948 1102 96-97 0.5503355901504783 1102 98-99 0.5345651330553984 1102 100 0.6501639653772848 1103 1 0.07912680314312581 1103 2 0.15225967990075162 1103 3 0.16590222452811787 1103 4 0.15893882344686716 1103 5 0.17532618525320487 1103 6 0.05300966381624761 1103 7 0.11888639193283268 1103 8 -0.0290195136879845 1103 9 -0.16683705304862428 1103 10-11 -0.06728833284666536 1103 12-13 0.10799203915366462 1103 14-15 0.26558074789495123 1103 16-17 0.32739409312571155 1103 18-19 0.4090067021760717 1103 20-21 0.4009057915623302 1103 22-23 0.316284475536996 1103 24-25 0.2906956505668532 1103 26-27 0.33870655917915116 1103 28-29 0.3664639732977335 1103 30-31 0.3555874883476804 1103 32-33 0.3505950503922435 1103 34-35 0.37354996405802865 1103 36-37 0.34888731168689446 1103 38-39 0.4112795991317384 1103 40-41 0.42741537423422926 1103 42-43 0.5136462518763878 1103 44-45 0.5607383878417451 1103 46-47 0.5530074131237299 1103 48-49 0.5419512941792064 1103 50-51 0.5191094177866873 1103 52-53 0.37246516380640315 1103 54-55 0.37017533874198705 1103 56-57 0.3887951141312769 1103 58-59 0.3549952180198872 1103 60-61 0.3792375486801802 1103 62-63 0.3589239818052832 1103 64-65 0.3133410866590083 1103 66-67 0.28627369285150195 1103 68-69 0.4058110079964763 1103 70-71 0.4815771429791553 1103 72-73 0.5068350556677501 1103 74-75 0.5086035915902727 1103 76-77 0.4472989389545532 1103 78-79 0.4196706401307466 1103 80-81 0.4730918325671851 1103 82-83 0.4230155415204564 1103 84-85 0.37758583932721024 1103 86-87 0.38647004031066956 1103 88-89 0.5053239850315663 1103 90-91 0.49161527159737517 1103 92-93 0.3907452112215637 1103 94-95 0.42192520978758097 1103 96-97 0.4815218157339878 1103 98-99 0.5068207966523204 1103 100 0.4494196804224728 1104 1 -0.3829773150242666 1104 2 -0.28065804157853336 1104 3 -0.23000731827827536 1104 4 -0.23366852735427557 1104 5 -0.13140360925639527 1104 6 -0.05932614554416915 1104 7 -0.00922119890341122 1104 8 -0.3545940941715102 1104 9 -0.09197777099056736 1104 10-11 0.14199733935597436 1104 12-13 0.18478065023110446 1104 14-15 0.20418276168094707 1104 16-17 0.3028574061077336 1104 18-19 0.2917943091058248 1104 20-21 0.2925810720394111 1104 22-23 0.3570336338718363 1104 24-25 0.3017180890417279 1104 26-27 0.3743730809713455 1104 28-29 0.3975018636786558 1104 30-31 0.3939868835147564 1104 32-33 0.42815769626279376 1104 34-35 0.4931965361522046 1104 36-37 0.4462209530712684 1104 38-39 0.4691870256809523 1104 40-41 0.4776518568753545 1104 42-43 0.42602912230544376 1104 44-45 0.48757030188850337 1104 46-47 0.486277964091947 1104 48-49 0.47949091230901786 1104 50-51 0.5333209968248696 1104 52-53 0.4008435434353075 1104 54-55 0.42302531983838776 1104 56-57 0.42644216968873394 1104 58-59 0.38284016346883476 1104 60-61 0.4127384593123651 1104 62-63 0.35708328429382163 1104 64-65 0.30971243869359455 1104 66-67 0.4218421133424073 1104 68-69 0.3925294428668664 1104 70-71 0.34179147699718015 1104 72-73 0.37052159584224853 1104 74-75 0.33172774736744515 1104 76-77 0.2898150928694321 1104 78-79 0.3104937420258196 1104 80-81 0.3515426134446997 1104 82-83 0.383110798799116 1104 84-85 0.4038494526692702 1104 86-87 0.41278398442202047 1104 88-89 0.3607321161601291 1104 90-91 0.354003031154285 1104 92-93 0.3824162143544072 1104 94-95 0.4948474512681358 1104 96-97 0.42090012637014595 1104 98-99 0.45005946187003687 1104 100 0.46677736952966953 1105 1 -0.031091922679362227 1105 2 0.10822434779823453 1105 3 0.08735463329394122 1105 4 0.06972337609865065 1105 5 0.09979987427566783 1105 6 0.08450154197102933 1105 7 0.11539418883828745 1105 8 -0.09277665059703111 1105 9 -0.11427654875650717 1105 10-11 0.07842601453251774 1105 12-13 0.13240748482322573 1105 14-15 0.16849555342719924 1105 16-17 0.18889271887634607 1105 18-19 0.2104674622533267 1105 20-21 0.2489463958423599 1105 22-23 0.17474428507554052 1105 24-25 0.21011478777985815 1105 26-27 0.23238096534724662 1105 28-29 0.1937652377150627 1105 30-31 0.22439471064335237 1105 32-33 0.22618179297011665 1105 34-35 0.2646653197696409 1105 36-37 0.29525486591271033 1105 38-39 0.2662310185399335 1105 40-41 0.2683300856418569 1105 42-43 0.28437772304112485 1105 44-45 0.380195684492854 1105 46-47 0.37831437477814944 1105 48-49 0.2719546844639211 1105 50-51 0.2938441827134639 1105 52-53 0.2762332359574202 1105 54-55 0.18722061717848248 1105 56-57 0.19043962704088813 1105 58-59 0.23456651936463402 1105 60-61 0.27122025972690267 1105 62-63 0.17114732213201478 1105 64-65 0.1670060863607432 1105 66-67 0.11852249478602772 1105 68-69 0.15899977910969199 1105 70-71 0.16585095808064665 1105 72-73 0.182328303125864 1105 74-75 0.17734399739925522 1105 76-77 0.22827185774389136 1105 78-79 0.27051864596287345 1105 80-81 0.2570710436170529 1105 82-83 0.2689831560579776 1105 84-85 0.18492114448131503 1105 86-87 0.15729698501066025 1105 88-89 0.22128647067680873 1105 90-91 0.20099922598830489 1105 92-93 0.25542548878726024 1105 94-95 0.3644685768049385 1105 96-97 0.3506198051542633 1105 98-99 0.27325955585007833 1105 100 0.17478383627005556 1106 1 0.063425839708394 1106 2 0.061614265245538036 1106 3 0.10115629943103244 1106 4 0.04889361254416258 1106 5 0.08043573021574701 1106 6 0.05933259092316945 1106 7 0.07176081663249079 1106 8 -0.1188830325020902 1106 9 -0.10205695315215024 1106 10-11 0.060716029743240085 1106 12-13 0.06028991141887019 1106 14-15 0.07392316995725423 1106 16-17 0.09090845862047559 1106 18-19 0.13470871442360988 1106 20-21 0.14741731053199914 1106 22-23 0.19254091498249437 1106 24-25 0.14463506218286426 1106 26-27 0.21665494580454947 1106 28-29 0.205063761060444 1106 30-31 0.26954388719587996 1106 32-33 0.2683021334729574 1106 34-35 0.19898346844212966 1106 36-37 0.20313724260076782 1106 38-39 0.20828276383601008 1106 40-41 0.15646195036124055 1106 42-43 0.19941324338041255 1106 44-45 0.2371472292158714 1106 46-47 0.21889969651295615 1106 48-49 0.2015420097111189 1106 50-51 0.15400118089404913 1106 52-53 0.017354676090775456 1106 54-55 0.05034988947593888 1106 56-57 0.12874109437942138 1106 58-59 0.1503581396588345 1106 60-61 0.16735217213575737 1106 62-63 0.11763266344168954 1106 64-65 0.11909064312314399 1106 66-67 0.09785285140901578 1106 68-69 0.11326058396381455 1106 70-71 0.048984577883537384 1106 72-73 0.06273059155512328 1106 74-75 0.072362277439602 1106 76-77 0.09710903312969776 1106 78-79 0.07298011591878861 1106 80-81 0.04742673499518446 1106 82-83 0.08444388904516842 1106 84-85 0.05185684077256525 1106 86-87 0.029427645293587545 1106 88-89 -0.06945960894353931 1106 90-91 -0.03646551291460298 1106 92-93 -0.05686555784694747 1106 94-95 0.04861163302361504 1106 96-97 0.04261457262094481 1106 98-99 0.08271654465913159 1106 100 0.03857466137002419 1107 1 -0.023470999707097207 1107 2 0.07861627693907991 1107 3 0.07165020543614276 1107 4 0.031267217231047084 1107 5 0.0074243646942449 1107 6 0.04131755872835896 1107 7 0.02624699007286324 1107 8 -0.2074360174108918 1107 9 -0.08542411959472673 1107 10-11 -0.005431509873240259 1107 12-13 -0.016089520848467487 1107 14-15 0.022895524011730117 1107 16-17 0.043428088190523795 1107 18-19 0.04501308865994247 1107 20-21 0.0405009639482401 1107 22-23 0.042428933040213224 1107 24-25 0.1384064253298689 1107 26-27 0.19449336253340022 1107 28-29 0.19469619522038784 1107 30-31 0.11529234856506321 1107 32-33 0.2400875800888258 1107 34-35 0.16084159212765314 1107 36-37 0.17782361156118043 1107 38-39 0.1820339024416313 1107 40-41 0.23846038684655468 1107 42-43 0.27047357822507223 1107 44-45 0.21839402009210573 1107 46-47 0.24055884959302176 1107 48-49 0.17973888881527955 1107 50-51 0.2414975648852291 1107 52-53 0.23429758520730815 1107 54-55 0.3195553575089889 1107 56-57 0.2748308998081086 1107 58-59 0.3166205217428697 1107 60-61 0.31926135305886305 1107 62-63 0.2574146519604952 1107 64-65 0.2862070130573926 1107 66-67 0.2679992708473904 1107 68-69 0.30645346640304183 1107 70-71 0.30899045316929374 1107 72-73 0.29642331088163587 1107 74-75 0.2800236787670016 1107 76-77 0.3274496024262348 1107 78-79 0.2507131146692956 1107 80-81 0.269243790863662 1107 82-83 0.21704775256565512 1107 84-85 0.18477114750588441 1107 86-87 0.27015214550422684 1107 88-89 0.27936207882375896 1107 90-91 0.2419002716880172 1107 92-93 0.3009885298974737 1107 94-95 0.2894421699469021 1107 96-97 0.28753740927921356 1107 98-99 0.37679405574747804 1107 100 0.3204161186847969 1108 1 0.1146755296121853 1108 2 0.08461320588511967 1108 3 0.11924998638870932 1108 4 0.06517005256792174 1108 5 -0.0031170120501400334 1108 6 -0.04429232650851844 1108 7 0.0485759495725091 1108 8 -0.3040266186445848 1108 9 -0.1884819259766033 1108 10-11 -0.049752363027899094 1108 12-13 0.02939872686974354 1108 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2214 94-95 -0.33932219740789904 2214 96-97 -0.3951202937956104 2214 98-99 -0.41129426426368454 2214 100 -0.3845136735738244 2215 1 -0.2843879257579083 2215 2 -0.2897094532875073 2215 3 -0.25813777110368363 2215 4 -0.23716513607451617 2215 5 -0.32633807979332374 2215 6 -0.20886903350237418 2215 7 -0.16734056072640158 2215 8 0.40517920195575385 2215 9 0.22480459859126967 2215 10-11 -0.07579699332860912 2215 12-13 -0.2445813345588519 2215 14-15 -0.3647189085979008 2215 16-17 -0.44771748676744494 2215 18-19 -0.5025954758847462 2215 20-21 -0.6283784368517189 2215 22-23 -0.5484621380010353 2215 24-25 -0.561852419569334 2215 26-27 -0.6222438076339216 2215 28-29 -0.6378620504094954 2215 30-31 -0.6623474201998647 2215 32-33 -0.6395596388043714 2215 34-35 -0.7146272284524642 2215 36-37 -0.7543281957652255 2215 38-39 -0.7820561491394074 2215 40-41 -0.7398399335238537 2215 42-43 -0.8720104380784548 2215 44-45 -0.9175222887137409 2215 46-47 -0.9299322054403873 2215 48-49 -0.77194756076738 2215 50-51 -0.8576026798975462 2215 52-53 -0.7303059773316178 2215 54-55 -0.7114442321981471 2215 56-57 -0.7051464106355994 2215 58-59 -0.7975662585725303 2215 60-61 -0.6574601836754681 2215 62-63 -0.6522291243640908 2215 64-65 -0.6389234946378295 2215 66-67 -0.6765486946874191 2215 68-69 -0.749399353507286 2215 70-71 -0.6869375726042009 2215 72-73 -0.7202694250185147 2215 74-75 -0.6647898310780036 2215 76-77 -0.656397336447796 2215 78-79 -0.6234311166477298 2215 80-81 -0.6797826093113883 2215 82-83 -0.8127076924008954 2215 84-85 -0.7497759387772973 2215 86-87 -0.7917701270303183 2215 88-89 -0.8154361344013168 2215 90-91 -0.7096436035210374 2215 92-93 -0.7076941352181372 2215 94-95 -0.7267701466954186 2215 96-97 -0.8191237362080379 2215 98-99 -0.8354695587785805 2215 100 -0.9834108664100079 2216 1 -0.21427977513396712 2216 2 -0.25509801028292145 2216 3 -0.2997408792340224 2216 4 -0.17643444582008527 2216 5 -0.24029833191606542 2216 6 -0.1661713940744889 2216 7 -0.18193340684244674 2216 8 0.2706529645396998 2216 9 0.17959026725421268 2216 10-11 -0.2534108789192331 2216 12-13 -0.3782951856615 2216 14-15 -0.5143780862438021 2216 16-17 -0.5893154068413935 2216 18-19 -0.7423397932185907 2216 20-21 -0.733685999435636 2216 22-23 -0.702299057812553 2216 24-25 -0.7410491792243192 2216 26-27 -0.7706483866137717 2216 28-29 -0.7473735538746382 2216 30-31 -0.8081225332678343 2216 32-33 -0.8159308782872472 2216 34-35 -0.8003222580877036 2216 36-37 -0.8808349449549411 2216 38-39 -0.9116100576970396 2216 40-41 -0.9242211637948685 2216 42-43 -0.9749463223590809 2216 44-45 -0.975908177768936 2216 46-47 -1.0393014088534187 2216 48-49 -1.0014740236668302 2216 50-51 -1.0479017509963953 2216 52-53 -0.8829475120969619 2216 54-55 -0.8775074561920633 2216 56-57 -0.8375880572768821 2216 58-59 -0.8643277251785761 2216 60-61 -0.8560434760147473 2216 62-63 -0.8328923337048053 2216 64-65 -0.78697679762071 2216 66-67 -0.8320257639304636 2216 68-69 -0.8733447017589597 2216 70-71 -0.753295818041007 2216 72-73 -0.7945216891237941 2216 74-75 -0.7600843479291086 2216 76-77 -0.6914679515161097 2216 78-79 -0.7541384793848707 2216 80-81 -0.6907512782318577 2216 82-83 -0.7505381079532292 2216 84-85 -0.7154358511501826 2216 86-87 -0.7867172314681525 2216 88-89 -0.8460176916167903 2216 90-91 -0.8250547605428942 2216 92-93 -0.862950308262235 2216 94-95 -0.9757292080849211 2216 96-97 -1.0136640272483142 2216 98-99 -1.0385056313410779 2216 100 -1.0375261063870127 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 2.0 11 8.0 12 13.0 13 39.0 14 136.0 15 449.0 16 1278.0 17 2621.0 18 4182.0 19 6132.0 20 8085.0 21 10324.0 22 12522.0 23 15174.0 24 18347.0 25 21319.0 26 24706.0 27 28709.0 28 33754.0 29 39219.0 30 46434.0 31 55927.0 32 69791.0 33 90562.0 34 126135.0 35 200939.0 36 466312.0 37 865909.0 38 74916.0 39 7533.0 40 79.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.111501505808114 23.37135200057364 24.006390721353792 32.510755772264446 2 20.04657742836491 23.050628866981874 31.964510813769603 24.938282890883613 3 17.559003672773617 26.877031093998987 26.11128737078523 29.452677862442172 4 18.99862736653795 20.058732400109882 26.783740096805836 34.158900136546336 5 18.555391623447747 26.266808767718565 28.29067313949507 26.887126469338618 6 15.353121434480485 18.119583659355698 25.299380400655718 41.227914505508096 7 23.560848519285628 17.58807075594719 23.85512722501499 34.99595349975219 8 16.863599332842792 20.998199464230822 24.1265519426338 38.011649260292586 9 15.20547527781039 14.9517960097833 27.95912093703144 41.88360777537487 10-11 22.136644921651616 19.407896505918483 28.081410252962826 30.37404831946708 12-13 18.787217985810113 31.58258673291961 20.289426417286 29.340768863984284 14-15 22.21199261496545 29.39906970074478 18.569799331403424 29.81913835288635 16-17 32.406960533762806 30.0713449333267 16.09697372188785 21.424720811022638 18-19 33.00321994984218 21.62035780855201 16.164098914815735 29.21232332679007 20-21 23.037540176805955 18.679030311992715 26.97876020734537 31.304669303855963 22-23 21.775366605184903 20.429534369740217 29.744290530912064 28.050808494162816 24-25 21.84687076668132 23.78157722447103 26.35189484632451 28.01965716252314 26-27 27.41956480871736 24.624675358721472 19.073838847968506 28.881920984592664 28-29 25.521992739927775 28.871256168159782 22.75842861403245 22.848322477879993 30-31 25.62994942658744 25.05869908180946 22.565497733932002 26.7458537576711 32-33 26.91756497267551 26.249812347217027 21.689188735007384 25.143433945100078 34-35 26.39153253263324 26.539347539138937 21.662863927514405 25.406256000713416 36-37 26.847806311162977 25.025027581874028 21.48370316794964 26.64346293901336 38-39 27.770633960906256 25.050621019133256 20.26350937595156 26.915235644008927 40-41 28.129159089748057 25.774100426697398 20.218273511152297 25.878466972402244 42-43 27.962382318712898 26.115712034243355 20.647825710927393 25.27407993611635 44-45 28.426864986753564 25.03103232560057 20.53443714307224 26.007665544573623 46-47 29.35382697438646 24.15180183853942 20.789884537860164 25.704486649213955 48-49 28.453525229890708 24.47469796211108 21.08931484602963 25.98246196196858 50-51 28.27337547406077 24.26767542629889 21.511046083828266 25.947903015812074 52-53 27.392208299862293 24.703489894135874 21.79796430313289 26.106337502868946 54-55 27.258218358727937 25.353236448225186 21.718599704643893 25.669945488402984 56-57 27.44886413285803 25.77680137841102 20.760366650160986 26.013967838569968 58-59 27.239167224388254 26.063459493056286 21.21891089576996 25.478462386785505 60-61 26.687448058656944 25.683183406912935 21.516851905918195 26.112516628511926 62-63 26.520768063977094 25.59489463198223 21.650352922300627 26.23398438174005 64-65 26.32165357453863 25.632821046960903 21.665854711216504 26.37967066728396 66-67 25.949931293009097 25.430796866638524 22.2553954932158 26.363876347136582 68-69 25.917325672883273 25.072713097724748 22.544298461747385 26.465662767644595 70-71 25.61657149118905 24.886704841433836 23.127069626858194 26.369654040518913 72-73 25.386389793196418 24.521645716211566 23.893961177719135 26.198003312872874 74-75 25.072078205244846 24.24543654092738 24.38098793639639 26.301497317431384 76-77 25.236595463381565 24.199100295233134 24.50374503509745 26.06055920628785 78-79 25.71243601689275 23.96810222776171 24.623384259875504 25.696077495470032 80-81 25.51808348270598 23.56238682207248 25.177471281573577 25.74205841364797 82-83 25.38844173788491 23.373886909214278 25.57479158889018 25.662879764010636 84-85 25.039921085548766 23.453699383805777 25.62507927074944 25.88130025989602 86-87 25.113599644333064 23.586867034075638 25.508145247996573 25.791388073594735 88-89 24.934915878016064 24.141201061423544 25.436967781286874 25.486915279273514 90-91 24.832061371460146 24.6439727963958 25.31386748785681 25.21009834428724 92-93 24.626789753260645 25.11953349111291 24.956921335333288 25.296755420293156 94-95 24.464594704454303 25.89012376828884 24.664429154228365 24.98085237302849 96-97 23.87084399807928 26.895684500235607 24.89138820317582 24.342083298509294 98-99 23.649772654143 27.926500917360475 24.51781436065756 23.905912067838962 100 23.3884430485886 28.914786317087238 24.004433156894407 23.692337477429756 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 924.0 1 979.5 2 899.5 3 569.5 4 274.5 5 135.0 6 83.5 7 65.5 8 49.5 9 48.5 10 66.5 11 103.5 12 148.5 13 203.0 14 264.0 15 298.5 16 319.5 17 358.5 18 428.5 19 507.0 20 639.0 21 780.0 22 907.0 23 1026.5 24 1174.0 25 1375.5 26 1562.5 27 1757.0 28 1969.5 29 2262.5 30 2601.5 31 2942.5 32 3345.0 33 3793.0 34 4165.0 35 4513.0 36 4902.0 37 5233.5 38 5538.0 39 5813.5 40 6025.0 41 6502.0 42 7537.0 43 9760.5 44 14192.0 45 21040.5 46 30457.5 47 42887.0 48 59968.0 49 85044.5 50 121928.5 51 169301.5 52 219347.0 53 259175.0 54 273867.5 55 257198.5 56 213632.5 57 156185.5 58 100838.0 59 57899.5 60 29833.5 61 14098.0 62 6373.5 63 2817.0 64 1291.5 65 630.0 66 356.5 67 236.0 68 162.5 69 115.5 70 80.0 71 59.5 72 45.5 73 26.0 74 17.0 75 16.5 76 13.0 77 10.0 78 5.0 79 2.5 80 3.0 81 4.0 82 2.0 83 1.0 84 1.5 85 1.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008872724795860112 2 2.2405870696616444E-4 3 8.962348278646579E-5 4 0.003629751052851864 5 0.012636911072891674 6 0.0051981620016150155 7 0.0 8 0.0 9 0.0 10-11 0.010844441417162358 12-13 0.004929291553255617 14-15 8.06611345078192E-4 16-17 2.2405870696616444E-4 18-19 6.721761208984934E-5 20-21 6.945819915951097E-4 22-23 8.962348278646579E-5 24-25 2.2405870696616444E-4 26-27 0.002531863388717658 28-29 0.0016804403022462333 30-31 0.002218181198965028 32-33 0.0019269048799090143 34-35 0.002352616423144727 36-37 8.962348278646578E-4 38-39 0.0010978876641342058 40-41 0.0 42-43 4.481174139323289E-5 44-45 6.273643795052604E-4 46-47 1.7924696557293157E-4 48-49 3.8089980184247954E-4 50-51 0.03134581310456641 52-53 0.034684287838362256 54-55 6.497702502018769E-4 56-57 1.3443522417969867E-4 58-59 3.8089980184247954E-4 60-61 0.00333847473379585 62-63 0.011628646891543935 64-65 0.014294945504441292 66-67 0.015415239039272114 68-69 0.016020197548080758 70-71 0.014720657047677003 72-73 0.011740676245027016 74-75 0.013488334159363098 76-77 0.012121576046869496 78-79 0.01366758112493603 80-81 0.012614505202195057 82-83 0.012143981917566113 84-85 0.012592099331498442 86-87 0.011516617538060853 88-89 0.00963452439954507 90-91 0.007281907976400345 92-93 0.007842054743815757 94-95 0.00945527743397214 96-97 0.004548391751413138 98-99 0.0069906316573443315 100 0.008559042606107481 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2231558.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.87484905789941 #Duplication Level Percentage of deduplicated Percentage of total 1 98.3457839965658 96.25578764144656 2 1.5602489424705603 3.0541825947410652 3 0.05044181424180127 0.148109548653687 4 0.01226016129400209 0.04799845744303821 5 0.008245538040295836 0.04035153955475612 6 0.003796936047461488 0.02229747255166742 7 0.0029937807140227443 0.020511108484819848 8 0.0025282456657331606 0.01979613303319372 9 0.001215623548751436 0.010708107421074734 >10 0.01107763881774609 0.19037063521526787 >50 6.729228164237952E-4 0.046322464581225214 >100 6.428285568668253E-4 0.09503225435545531 >500 9.157122059684867E-5 0.048532042518142554 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0038089980184247956 0.0 0.0 0.0 0.0 2 0.008424607381927783 0.0 0.0 0.0 0.0 3 0.013533145900756332 0.0 0.0 0.0 0.0 4 0.02307804681751494 0.0 0.0 0.0 0.0 5 0.040599437702268995 0.0 0.0 0.0 0.0 6 0.07631439559267561 0.0 0.0 0.0 0.0 7 0.11588316324290025 0.0 0.0 0.0 0.0 8 0.32582617167019634 0.0 0.0 0.0 0.0 9 1.2203581533619112 0.0 0.0 0.0 0.0 10-11 2.951480535123891 0.0 0.0 0.0 0.0 12-13 4.566943812349937 0.0 0.0 0.0 0.0 14-15 6.119737869237547 0.0 0.0 0.0 0.0 16-17 7.449167801150587 0.0 0.0 0.0 0.0 18-19 8.792175690705776 0.0 0.0 0.0 0.0 20-21 10.464617097113317 0.0 0.0 0.0 0.0 22-23 12.515067948043475 0.0 0.0 0.0 0.0 24-25 15.086701757247628 0.0 0.0 0.0 0.0 26-27 17.95064255555984 0.0 0.0 0.0 0.0 28-29 21.195460749843832 0.0 0.0 4.4811741393232886E-5 0.0 30-31 24.69942524460489 0.0 0.0 4.4811741393232886E-5 0.0 32-33 28.64532313298601 0.0 0.0 4.4811741393232886E-5 0.0 34-35 32.31625617617826 0.0 0.0 4.4811741393232886E-5 0.0 36-37 35.190481269140214 0.0 0.0 4.4811741393232886E-5 0.0 38-39 37.35842850600343 0.0 4.4811741393232886E-5 4.4811741393232886E-5 0.0 40-41 39.60360877915788 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 42-43 41.5092504877758 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 44-45 43.06542783113861 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 46-47 44.42376133625028 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 48-49 45.66397109104939 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 50-51 46.87899664718551 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 52-53 48.143315118854176 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 54-55 49.426969856934036 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 56-57 50.70659153828849 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 58-59 51.92851810259917 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 60-61 53.106551566215174 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 62-63 54.27270991836197 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 64-65 55.40875478029251 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 66-67 56.4862306962221 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 68-69 57.494293224733575 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 70-71 58.46137541574093 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 72-73 59.42175825141 0.0 4.4811741393232886E-5 8.962348278646577E-5 0.0 74-75 60.355724565527765 2.2405870696616443E-5 4.4811741393232886E-5 8.962348278646577E-5 0.0 76-77 61.277860579917714 4.4811741393232886E-5 4.4811741393232886E-5 8.962348278646577E-5 0.0 78-79 62.1573134106306 4.4811741393232886E-5 4.4811741393232886E-5 8.962348278646577E-5 0.0 80-81 63.02504797096916 4.4811741393232886E-5 4.4811741393232886E-5 8.962348278646577E-5 0.0 82-83 63.877098421820094 4.4811741393232886E-5 4.4811741393232886E-5 8.962348278646577E-5 0.0 84-85 64.71505558000285 4.4811741393232886E-5 4.4811741393232886E-5 8.962348278646577E-5 0.0 86-87 65.5405102623369 4.4811741393232886E-5 4.4811741393232886E-5 8.962348278646577E-5 0.0 88 66.15382616091537 4.4811741393232886E-5 4.4811741393232886E-5 8.962348278646577E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCGTT 90 3.6379788E-12 52.22418 3 TGCCCTA 2475 0.0 46.913113 6 CCACGTT 110 3.8198777E-11 42.72887 3 CCGCCTA 1205 0.0 41.741615 6 CCCCCTA 7750 0.0 41.731045 6 CCACCTA 1310 0.0 40.548958 6 CCCCTAC 16050 0.0 40.26811 7 GCCCCTA 1900 0.0 40.080574 6 CACCCTA 3335 0.0 39.3261 6 TTGCCCT 1130 0.0 39.10144 5 GTCCCTA 1095 0.0 39.06608 6 GCCCTAC 7275 0.0 38.959904 7 CCCGCTA 925 0.0 38.114662 6 ACCCGTG 185 0.0 38.109535 4 CGCCCTA 2470 0.0 37.682594 6 CTCCCTA 5815 0.0 37.671135 6 CATGCTC 250 0.0 37.601406 4 ATTCGCG 100 3.328205E-8 37.601406 4 TCCCCCT 3340 0.0 37.15359 5 ACCCCTA 2400 0.0 36.822994 6 >>END_MODULE