##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248475_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2818868
Sequences flagged as poor quality	0
Sequence length	100
%GC	19
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.718496219049634	31.0	28.0	34.0	12.0	34.0
2	29.603070452394366	33.0	30.0	34.0	16.0	34.0
3	30.78312499911312	33.0	31.0	34.0	26.0	34.0
4	34.66149709741641	37.0	35.0	37.0	32.0	37.0
5	34.50201428374795	37.0	35.0	37.0	31.0	37.0
6	34.08925462277765	37.0	35.0	37.0	29.0	37.0
7	34.5225012309906	37.0	35.0	37.0	31.0	37.0
8	34.27243560180895	37.0	35.0	37.0	30.0	37.0
9	35.53428184647171	39.0	35.0	39.0	27.0	39.0
10-11	36.89827015667282	39.0	37.0	39.0	32.5	39.0
12-13	37.0107975967658	39.0	37.0	39.0	32.0	39.0
14-15	38.56345490459291	41.0	39.0	41.0	32.0	41.0
16-17	38.332810191892634	40.5	39.0	41.0	32.0	41.0
18-19	36.841788086565245	38.0	36.0	40.5	31.5	41.0
20-21	35.921377482024695	35.0	35.0	40.0	30.0	41.0
22-23	35.32547320413727	35.0	35.0	39.0	30.0	41.0
24-25	34.95625495766386	35.0	35.0	39.0	27.5	41.0
26-27	34.82429631327185	35.0	35.0	39.0	26.0	41.0
28-29	34.691181176273595	35.0	35.0	39.0	25.0	41.0
30-31	34.617882071810385	35.0	35.0	39.0	25.0	41.0
32-33	34.56625478738273	35.0	35.0	39.0	25.0	41.0
34-35	34.504283989175796	35.0	35.0	39.0	25.0	41.0
36-37	34.41481580549355	35.0	35.0	39.0	25.0	41.0
38-39	34.315863850311544	35.0	34.0	38.5	25.0	41.0
40-41	34.21686205242672	35.0	34.0	38.0	25.0	41.0
42-43	34.13778420983175	35.0	34.0	38.0	25.0	41.0
44-45	34.03795920915772	35.0	33.5	38.0	25.0	41.0
46-47	33.95241228039056	35.0	33.0	38.0	25.0	41.0
48-49	33.874225753032775	35.0	33.0	38.0	25.0	40.0
50-51	33.75868469186922	35.0	33.0	37.5	25.0	40.0
52-53	33.592976506881485	35.0	33.0	37.0	25.0	40.0
54-55	33.53157916582117	35.0	33.0	36.0	26.0	40.0
56-57	33.46490825395159	35.0	33.0	36.0	26.0	40.0
58-59	33.49474168354105	35.0	33.0	36.0	26.5	40.0
60-61	33.3650671120464	35.0	33.0	35.0	26.0	40.0
62-63	33.19192562404483	35.0	33.0	35.0	26.0	39.0
64-65	33.044682298000474	35.0	33.0	35.0	26.0	39.0
66-67	32.90881321863954	35.0	33.0	35.0	26.0	39.0
68-69	32.76871478196212	35.0	32.0	35.0	26.0	38.5
70-71	32.64251837971838	35.0	32.0	35.0	25.5	37.5
72-73	32.603078611698024	35.0	32.0	35.0	26.0	37.0
74-75	32.59306661397412	35.0	33.0	35.0	26.0	37.0
76-77	32.45897732706888	35.0	32.5	35.0	25.0	36.0
78-79	32.26279662616341	35.0	32.0	35.0	25.0	36.0
80-81	32.0647838068331	35.0	32.0	35.0	25.0	36.0
82-83	31.87375038490628	35.0	31.5	35.0	25.0	35.0
84-85	31.689616363731822	35.0	31.0	35.0	25.0	35.0
86-87	31.50214554211123	35.0	31.0	35.0	24.0	35.0
88-89	31.302556735540648	35.0	30.5	35.0	24.0	35.0
90-91	31.08179737398133	35.0	30.0	35.0	22.0	35.0
92-93	30.88136922339038	35.0	30.0	35.0	20.0	35.0
94-95	30.68904361608986	35.0	30.0	35.0	19.5	35.0
96-97	30.502431472491793	35.0	30.0	35.0	18.0	35.0
98-99	30.332470693909755	35.0	29.5	35.0	17.0	35.0
100	29.867927480109035	34.0	28.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.45538867193094745
1101	2	0.2441461094124051
1101	3	0.17134535415801366
1101	4	0.12066347490592477
1101	5	0.018714273664620862
1101	6	0.023365580584155055
1101	7	-0.07501847294382458
1101	8	0.09544723410939326
1101	9	0.18305098821927857
1101	10-11	0.1906998126342856
1101	12-13	0.14417969859128021
1101	14-15	0.2951163462605635
1101	16-17	0.3126402708090339
1101	18-19	0.3700671620225151
1101	20-21	0.3604037026168285
1101	22-23	0.32617804443223974
1101	24-25	0.28735359908590397
1101	26-27	0.36098389204165215
1101	28-29	0.3324315562096771
1101	30-31	0.3308331321803948
1101	32-33	0.34951577275847967
1101	34-35	0.39971228942329873
1101	36-37	0.36385535955666626
1101	38-39	0.37530581867657986
1101	40-41	0.4288637318982609
1101	42-43	0.46232182494490814
1101	44-45	0.4653621151378644
1101	46-47	0.47452324998543816
1101	48-49	0.5182694379847064
1101	50-51	0.4663089499459545
1101	52-53	0.41897046068745425
1101	54-55	0.44438163876231584
1101	56-57	0.4169281275204142
1101	58-59	0.35780967031536903
1101	60-61	0.38898706947234984
1101	62-63	0.4166583401045756
1101	64-65	0.40143258396003745
1101	66-67	0.4181233134372704
1101	68-69	0.42634461262600354
1101	70-71	0.41898748804421615
1101	72-73	0.43429371747194523
1101	74-75	0.4101996200344331
1101	76-77	0.4162715901813243
1101	78-79	0.34995795244766015
1101	80-81	0.3664877935314834
1101	82-83	0.28043701293857026
1101	84-85	0.27896243835707324
1101	86-87	0.19831977121226885
1101	88-89	0.23928477754567368
1101	90-91	0.20779927087964722
1101	92-93	0.1858602671809102
1101	94-95	0.21878594783123617
1101	96-97	0.22239113328328486
1101	98-99	0.29835304410767804
1101	100	0.335428241073906
1102	1	0.1021668540884697
1102	2	-0.014885082583177223
1102	3	-0.0025685091215805755
1102	4	0.03788286135660712
1102	5	-0.123124824317145
1102	6	-0.31242023706401767
1102	7	-0.07102383811280077
1102	8	-0.072336217342027
1102	9	0.05924655035399695
1102	10-11	0.09317449202804795
1102	12-13	0.1324679994037936
1102	14-15	0.21863524419580216
1102	16-17	0.281738337264521
1102	18-19	0.21145288277293872
1102	20-21	0.10626050137377518
1102	22-23	0.07781003787949459
1102	24-25	0.03659330977329489
1102	26-27	-0.008057871778412107
1102	28-29	0.031011758263197464
1102	30-31	0.02219985368760291
1102	32-33	0.03756230061082988
1102	34-35	0.044394922165530204
1102	36-37	0.041883621610082855
1102	38-39	0.057437174235033694
1102	40-41	0.03392131766170792
1102	42-43	0.03565938317304074
1102	44-45	-0.012599801366455665
1102	46-47	0.03878858967719623
1102	48-49	0.10057718769451895
1102	50-51	0.058481205809677306
1102	52-53	0.02928687398826213
1102	54-55	0.026276646291705674
1102	56-57	0.08456936428326856
1102	58-59	0.04943896967314032
1102	60-61	0.07277191047312925
1102	62-63	0.10812317376771574
1102	64-65	0.09586223434839525
1102	66-67	0.13997075734395992
1102	68-69	0.10391547069509954
1102	70-71	0.13767149403705048
1102	72-73	0.14280821474142158
1102	74-75	0.167551520214424
1102	76-77	0.13214553779263127
1102	78-79	0.13056095551055336
1102	80-81	0.09535568950831674
1102	82-83	0.09802905780043503
1102	84-85	0.09520923612970122
1102	86-87	0.034587600779630634
1102	88-89	0.09618640045495752
1102	90-91	0.1761386059924206
1102	92-93	0.11926911811179153
1102	94-95	0.1738702747498877
1102	96-97	0.19505171763545448
1102	98-99	0.16843912713804698
1102	100	0.07306011723399664
1103	1	-0.1925300202706488
1103	2	-0.12603066538982333
1103	3	0.0948863727696505
1103	4	0.12550369678736928
1103	5	0.07438371689507761
1103	6	0.001931329951290195
1103	7	0.1269196290833534
1103	8	0.059260791252782496
1103	9	0.11362948564120501
1103	10-11	0.15369853559871416
1103	12-13	0.1759410800197898
1103	14-15	0.21470561656003184
1103	16-17	0.21773415754716297
1103	18-19	0.0991895431006995
1103	20-21	0.05591760025141923
1103	22-23	0.03148838142033128
1103	24-25	0.030786916798291486
1103	26-27	0.05925194933387701
1103	28-29	0.025242361671288904
1103	30-31	-5.138863612401678E-4
1103	32-33	0.054248106351266756
1103	34-35	0.06606591770032821
1103	36-37	0.06293747669145944
1103	38-39	0.05295871836501931
1103	40-41	0.06589289278708321
1103	42-43	0.07855246537376104
1103	44-45	0.04452465330103195
1103	46-47	0.08361582530745437
1103	48-49	0.106222049217358
1103	50-51	0.1184376316444542
1103	52-53	0.1170201551188299
1103	54-55	0.11013302691515747
1103	56-57	0.167470976043802
1103	58-59	0.20126440617219288
1103	60-61	0.14824453198440324
1103	62-63	0.12300125466336453
1103	64-65	0.1267083328904519
1103	66-67	0.15991399265726614
1103	68-69	0.16229168487561196
1103	70-71	0.1853997413290216
1103	72-73	0.17268308539973987
1103	74-75	0.13783453963199577
1103	76-77	0.16019010707316284
1103	78-79	0.1438598443372925
1103	80-81	0.144049484137021
1103	82-83	0.1826346880090597
1103	84-85	0.19796596189736348
1103	86-87	0.1866622530718942
1103	88-89	0.10652251987811923
1103	90-91	0.17743843167245643
1103	92-93	0.18114746235177748
1103	94-95	0.2354557066812042
1103	96-97	0.31823976879416804
1103	98-99	0.2580251405243281
1103	100	0.3844965440475754
1104	1	-0.24604644022326028
1104	2	-0.09582623236087784
1104	3	-0.07757830984400016
1104	4	-0.027260076231485186
1104	5	0.003753954342883503
1104	6	-0.12659570738538406
1104	7	-0.07668885395257519
1104	8	-0.12600041286700758
1104	9	0.009309603556623358
1104	10-11	0.012312796083847388
1104	12-13	0.02280549266225762
1104	14-15	0.06012920145558809
1104	16-17	0.10665590214190246
1104	18-19	-0.0019469425136051655
1104	20-21	-0.0016371317800789598
1104	22-23	-0.030579047684774707
1104	24-25	-0.05919572202863321
1104	26-27	-0.09163473606843553
1104	28-29	-0.0584936933178426
1104	30-31	-0.03663457698673511
1104	32-33	-0.05748887983803286
1104	34-35	-0.046641148741848326
1104	36-37	0.06218492584262947
1104	38-39	0.0020639069234391627
1104	40-41	-0.0690380331339071
1104	42-43	0.013322092160692023
1104	44-45	0.029477376773286323
1104	46-47	-0.024765943978366067
1104	48-49	-0.02589231874562614
1104	50-51	0.015543100770855744
1104	52-53	0.0014335607775777248
1104	54-55	0.09796287616340038
1104	56-57	0.08876455501778935
1104	58-59	0.10538441788906283
1104	60-61	0.11495807207968056
1104	62-63	0.12386855836392385
1104	64-65	0.08401430105449492
1104	66-67	0.09224361143895976
1104	68-69	0.073026766112676
1104	70-71	0.08936412978555097
1104	72-73	0.12648353238679988
1104	74-75	0.09000071933111542
1104	76-77	0.10335347258213545
1104	78-79	0.14872033451865008
1104	80-81	0.20867891478443568
1104	82-83	0.21189096873735735
1104	84-85	0.1796180996084722
1104	86-87	0.24134764906943218
1104	88-89	0.34367847065079005
1104	90-91	0.3544484459177326
1104	92-93	0.2973738793367353
1104	94-95	0.35640260438727367
1104	96-97	0.4021979172212191
1104	98-99	0.29079009950567425
1104	100	0.36706330728416603
1105	1	0.3007713715997653
1105	2	0.1783938820425668
1105	3	0.13167474098587206
1105	4	0.27997746079135055
1105	5	0.23860474203563342
1105	6	0.1828079503309752
1105	7	0.2114349885194855
1105	8	0.1600259031596707
1105	9	0.20589228030619466
1105	10-11	0.18798781175088664
1105	12-13	0.1291872764847639
1105	14-15	0.1730029546049039
1105	16-17	0.13805311327748626
1105	18-19	0.18301533800158865
1105	20-21	0.15797538679166934
1105	22-23	0.12575843115760676
1105	24-25	0.17217318838593343
1105	26-27	0.21533593544751994
1105	28-29	0.22009116962566821
1105	30-31	0.19092335718465137
1105	32-33	0.2569845832336739
1105	34-35	0.23912600504625914
1105	36-37	0.25897728491645466
1105	38-39	0.2232648867280318
1105	40-41	0.17205825126334418
1105	42-43	0.21882755090119588
1105	44-45	0.20524479969633092
1105	46-47	0.2644961602694167
1105	48-49	0.22097601501058506
1105	50-51	0.2727486772211307
1105	52-53	0.2656307961544826
1105	54-55	0.29929655163692104
1105	56-57	0.28683210865347064
1105	58-59	0.2644347896564696
1105	60-61	0.25218620344386267
1105	62-63	0.29131108159407404
1105	64-65	0.28929901800669455
1105	66-67	0.2800651150133717
1105	68-69	0.2720213329751573
1105	70-71	0.2563123333290349
1105	72-73	0.2642636460768131
1105	74-75	0.2992972408867445
1105	76-77	0.26467394318342485
1105	78-79	0.24903817584423393
1105	80-81	0.2601202857846445
1105	82-83	0.2453008744755678
1105	84-85	0.19527321846862478
1105	86-87	0.16289152340170432
1105	88-89	0.17786370235391402
1105	90-91	0.21026781124385607
1105	92-93	0.19588715494478137
1105	94-95	0.16250801667801795
1105	96-97	0.18256050910187227
1105	98-99	0.19096318970724013
1105	100	0.13685014282692975
1106	1	0.07820936133614609
1106	2	0.05602893318452118
1106	3	0.2020099621844551
1106	4	-0.009502558111996962
1106	5	0.011649998707191855
1106	6	0.02395680007739287
1106	7	-0.026352561326284274
1106	8	0.060021801854176715
1106	9	0.0167924060494542
1106	10-11	0.0545283798352969
1106	12-13	0.0983821059008747
1106	14-15	0.16355387195130788
1106	16-17	0.10096755516659783
1106	18-19	0.15729024555810156
1106	20-21	0.17151737359052532
1106	22-23	0.18155618054664302
1106	24-25	0.17532520125668327
1106	26-27	0.1828251999340793
1106	28-29	0.20922740758667402
1106	30-31	0.2014248673503829
1106	32-33	0.19855934843695167
1106	34-35	0.19356306051609096
1106	36-37	0.15550035349515667
1106	38-39	0.21871574998658616
1106	40-41	0.2249941205163708
1106	42-43	0.18054686273224263
1106	44-45	0.19138699430671124
1106	46-47	0.22105333773772884
1106	48-49	0.2433052746815818
1106	50-51	0.2527333729075778
1106	52-53	0.24644924328458728
1106	54-55	0.24987943387181843
1106	56-57	0.216933787906207
1106	58-59	0.2294320141988493
1106	60-61	0.20533393734610428
1106	62-63	0.1842942899098574
1106	64-65	0.22311816031109544
1106	66-67	0.17062798095025045
1106	68-69	0.19957625809801982
1106	70-71	0.2686030606025227
1106	72-73	0.16802094177835158
1106	74-75	0.15597154947198533
1106	76-77	0.18983544639078787
1106	78-79	0.19784132636955576
1106	80-81	0.15319690268820096
1106	82-83	0.16100588738504484
1106	84-85	0.1289165346144152
1106	86-87	0.07238739466383137
1106	88-89	0.1207336008131783
1106	90-91	0.0919058509788222
1106	92-93	0.1314816927767346
1106	94-95	0.09000220514017343
1106	96-97	0.1482975344773898
1106	98-99	0.15830999254688294
1106	100	0.16842423291075193
1107	1	0.035067456067150005
1107	2	0.054559383778816084
1107	3	0.07250181991521387
1107	4	0.0421556002018022
1107	5	0.06487390954978167
1107	6	-0.030997307588734202
1107	7	0.10748553874142885
1107	8	0.015255126242408323
1107	9	0.053544217344693834
1107	10-11	0.030843147580938535
1107	12-13	0.053008615080067045
1107	14-15	0.1061306480854114
1107	16-17	0.06514374207993257
1107	18-19	0.04337766279086708
1107	20-21	0.08589023109355765
1107	22-23	0.12063990183988693
1107	24-25	0.09815278383624104
1107	26-27	0.12000666208262345
1107	28-29	0.12485808589865854
1107	30-31	0.14768859315140048
1107	32-33	0.12384901294664985
1107	34-35	0.12555693854162087
1107	36-37	0.11745689268354198
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2216	24-25	0.14721858986092684
2216	26-27	0.11132974253883532
2216	28-29	0.08543937324435547
2216	30-31	0.02509186226865978
2216	32-33	0.03290924101647619
2216	34-35	-0.032083759015904434
2216	36-37	0.011333373888120946
2216	38-39	-0.032412527627244
2216	40-41	-0.09086462276884077
2216	42-43	-0.13105116388997828
2216	44-45	-0.11766549747758859
2216	46-47	-0.11406179476901457
2216	48-49	-0.055894833625330875
2216	50-51	-0.10732297247543698
2216	52-53	-0.17829582447976122
2216	54-55	-0.17602300287258998
2216	56-57	-0.2681959305763968
2216	58-59	-0.2597959605746638
2216	60-61	-0.24366736652731902
2216	62-63	-0.3326040063514597
2216	64-65	-0.30727788519715205
2216	66-67	-0.3167861753437222
2216	68-69	-0.2993625920113345
2216	70-71	-0.3187746710445367
2216	72-73	-0.3271432834162198
2216	74-75	-0.3328443110441839
2216	76-77	-0.3541366774723542
2216	78-79	-0.36518523944704384
2216	80-81	-0.3930744867186746
2216	82-83	-0.40384465068435205
2216	84-85	-0.4282577508742307
2216	86-87	-0.3627764114861307
2216	88-89	-0.38966370182889776
2216	90-91	-0.3976639674942746
2216	92-93	-0.48705764664778783
2216	94-95	-0.46287574489359073
2216	96-97	-0.33576684366846976
2216	98-99	-0.30932606398545914
2216	100	-0.3346897520584413
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	3241.0
1	154.0
2	77.0
3	73.0
4	67.0
5	121.0
6	529.0
7	1284.0
8	2006.0
9	3096.0
10	4206.0
11	5313.0
12	5929.0
13	6008.0
14	6377.0
15	6959.0
16	7484.0
17	7763.0
18	8846.0
19	10180.0
20	11762.0
21	13148.0
22	14661.0
23	18319.0
24	31819.0
25	63592.0
26	28307.0
27	108787.0
28	38543.0
29	103626.0
30	52373.0
31	58067.0
32	75344.0
33	201105.0
34	186641.0
35	1109663.0
36	167152.0
37	208839.0
38	191911.0
39	55135.0
40	361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.897336384693032	44.043293208734966	21.086075133514754	19.973295273057253
2	9.47418981917348	56.55790122149739	18.463361828544144	15.504547130784987
3	7.8800470671161165	61.77836106389872	15.000760293089431	15.340831575895725
4	6.9014433769079595	62.372370494687345	14.758305065958785	15.96788106244591
5	7.033716279634879	61.71767367404636	14.942899104106022	16.30571094221274
6	7.116607738707962	59.7820056249738	16.035615362046414	17.065771274271818
7	9.627826484790317	63.74888730860465	12.892479023147532	13.730807183457502
8	7.87314086267595	60.76480072406496	12.048698199819055	19.313360213440028
9	6.862627738730096	58.463480105810596	12.744195034205156	21.929697121254154
10-11	9.715893590902116	69.03649341017204	12.285525111819634	8.962087887106215
12-13	9.483282879915656	68.96786232149188	12.498179485339618	9.05067531325285
14-15	9.793009073837634	68.59905801472588	12.291582738464445	9.316350172972028
16-17	9.570391315467901	68.79514905226065	12.471244565062946	9.163215067208503
18-19	9.766450618879304	68.58608686353783	12.679036970051902	8.968425547530957
20-21	9.799394503065496	68.46673283886548	12.48487501056884	9.248997647500175
22-23	10.038937723621917	68.52451410442258	12.691982019123731	8.744566152831764
24-25	9.943666865373862	68.27904884857588	12.749310623398232	9.027973662652023
26-27	10.463751624650312	68.13132840930548	12.621816577978864	8.783103388065346
28-29	10.425125971441558	68.3603187339033	12.674520205433767	8.540035089221371
30-31	10.690351597054216	68.51588172810823	12.411940871159237	8.381825803678321
32-33	10.834026533259653	68.49838419138251	12.21214884161452	8.45544043374332
34-35	10.762089685383348	68.70120557132435	12.18099716623505	8.35570757705725
36-37	10.975310595939556	68.55655864974676	12.129304116949378	8.338826637364308
38-39	11.088067972895823	68.4711249839685	12.276211653602836	8.164595389532852
40-41	11.313471930096613	68.4760128979932	12.341392891609217	7.869122280300968
42-43	11.321930382984528	68.67065561517387	12.376693816999557	7.630720184842044
44-45	11.350373559431137	68.6717369811936	12.245612439594778	7.732277019780478
46-47	11.226664402572116	68.56476417596136	12.323465457223355	7.885105964243174
48-49	11.120296424714375	68.4548009151367	12.493800801455123	7.931101858693798
50-51	11.477240230270928	68.26982409958276	12.515590890763406	7.7373447793829015
52-53	11.717056602285563	68.05754174037334	12.654522021058778	7.570879636282317
54-55	11.755069052799858	67.94436697899552	12.706141480527556	7.594422487677073
56-57	11.653854215142614	67.89100771944283	12.518303925086235	7.936834140328316
58-59	11.46536684349652	67.76624924021661	12.519312086515304	8.249071829771573
60-61	11.53921918132569	67.58369600105438	12.74181770642663	8.135267111193292
62-63	11.575961167345488	67.29641195096292	12.883556209500105	8.244070672191487
64-65	11.566128584528022	67.27040843057512	12.886620550414854	8.276842434482013
66-67	11.306106102194914	67.28894248572288	13.180446193643034	8.224505218439166
68-69	11.147643467643467	67.5762814962815	12.845465645465644	8.43060939060939
70-71	10.93590967415386	67.84513539553078	12.782723361556462	8.436231568758908
72-73	10.782364436323608	68.25175130703104	12.720004532449908	8.24587972419545
74-75	10.578686156899881	68.69222036485932	12.642235674486773	8.08685780375402
76-77	10.297741232985365	69.18960947992343	12.608794027770426	7.903855259320775
78-79	10.03126879710765	69.5607219723193	12.534797125764072	7.873212104808987
80-81	9.926461708515012	70.01179937231034	12.24266570623612	7.819073212938534
82-83	9.855199817290421	70.43134591675704	12.024165686047118	7.689288579905427
84-85	9.682976718547271	70.82217635711984	11.927655555685787	7.567191368647094
86-87	9.55571981041469	71.0420479021158	11.872488818672107	7.529743468797418
88-89	9.577263283368765	71.13867391766475	11.77243535042712	7.511627448539361
90-91	9.655127339428425	71.16826408541131	11.699264475782261	7.477344099378
92-93	9.802607696783145	71.19764164983636	11.526953232945864	7.472797420434631
94-95	9.967903736785066	71.10554861131091	11.409997794181113	7.5165498577229055
96-97	10.116243961352657	70.91364734299516	11.368375248650185	7.601733447001989
98-99	10.29573941781392	70.66168069194076	11.30909721922499	7.733482671020337
100	10.731246729653874	70.44896590456575	11.095784863532652	7.724002502247725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	819225.0
1	702646.5
2	343338.5
3	57777.0
4	13728.0
5	11512.0
6	8855.0
7	5790.5
8	3600.0
9	2662.0
10	2423.5
11	2330.5
12	2490.5
13	2844.5
14	3173.5
15	3554.0
16	3999.0
17	4439.0
18	4954.0
19	5546.5
20	6108.0
21	6776.5
22	7631.5
23	8651.0
24	9698.5
25	10806.5
26	12153.5
27	13495.5
28	14853.5
29	16634.5
30	18751.0
31	20790.5
32	22878.5
33	25213.5
34	27759.0
35	29809.5
36	31449.0
37	32866.0
38	34195.0
39	34959.5
40	35750.5
41	37478.0
42	39474.5
43	42575.5
44	46729.5
45	51486.0
46	56198.5
47	59496.0
48	61190.0
49	60667.0
50	57680.5
51	52445.5
52	45201.0
53	37482.0
54	30358.0
55	24075.5
56	18929.0
57	14865.0
58	11659.0
59	9155.5
60	7223.5
61	5791.5
62	4701.0
63	3901.0
64	3249.0
65	2756.0
66	2368.5
67	1988.0
68	1706.5
69	1438.5
70	1162.5
71	977.0
72	818.5
73	703.5
74	613.0
75	525.0
76	489.5
77	436.0
78	385.0
79	344.0
80	294.5
81	282.0
82	263.5
83	252.5
84	239.5
85	211.5
86	188.5
87	170.5
88	164.0
89	155.5
90	150.5
91	142.5
92	149.5
93	149.5
94	131.5
95	131.0
96	118.5
97	100.0
98	100.5
99	104.5
100	94.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15555889811087287
2	0.14637081268083502
3	0.14771887154701818
4	0.14473895194808697
5	0.14282328934877403
6	0.12650468202129367
7	0.12849129508724777
8	0.12952007685354547
9	0.12944912638690426
10-11	0.12842034462060656
12-13	0.1323226202858736
14-15	0.14055287441625505
16-17	0.1329611744856446
18-19	0.14124464146600693
20-21	0.1415461809492321
22-23	0.1414929680992512
24-25	0.14211378468236185
26-27	0.1430184031320374
28-29	0.14344410593188472
30-31	0.14182998281579698
32-33	0.14273460126547252
34-35	0.1440826601316557
36-37	0.14465026386478544
38-39	0.14541298138117856
40-41	0.1423088984656252
42-43	0.1392048155500719
44-45	0.13909838985011005
46-47	0.13867268705026273
48-49	0.1414752304825909
50-51	0.13916934031675127
52-53	0.1396837311999001
54-55	0.14165260664919394
56-57	0.13828245948373602
58-59	0.13909838985011005
60-61	0.14060608726623594
62-63	0.13532027750146514
64-65	0.1274093004709692
66-67	0.12808332990406077
68-69	0.12613219207142726
70-71	0.12435843040539678
72-73	0.12818975560402263
74-75	0.12934270068694242
76-77	0.12668205818789668
78-79	0.12208801547287777
80-81	0.12288620822259147
82-83	0.12313453485583575
84-85	0.11937416012385113
86-87	0.12274430728930903
88-89	0.12201706500623655
90-91	0.12760441425423255
92-93	0.12451806895533951
94-95	0.12724966192102646
96-97	0.1301231558199958
98-99	0.13379484246867893
100	0.13477041138499568
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2818868.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.48003507532852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.191667352943	38.24113595453095
2	6.714149446653818	5.570063090963958
3	0.7563893257922103	0.9412516729339491
4	0.1211574359154002	0.20102458765630668
5	0.04166649758803659	0.08641638907089243
6	0.026768222813177783	0.06662080927168941
7	0.01672952727290498	0.04857589646516346
8	0.013935005048081508	0.04624195985354407
9	0.010208336214007581	0.03810979297960027
>10	0.08328565748978631	0.6667849254929527
>50	0.008270388878241382	0.2430002629436056
>100	0.009000857834307782	0.9268374155907916
>500	0.001542974937063964	0.47319295900887026
>1k	0.0025716248951066067	2.424740566102486
>5k	8.572082983688689E-4	2.375174065887701
>10k+	0.0018001374265746248	47.65082965124761
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	567277	20.124283932415423	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	143859	5.103431590269569	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114391	4.058047414777847	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107853	3.826110339327702	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61090	2.1671820035560376	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40242	1.4275943392879695	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37460	1.3289022401900337	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36392	1.2910146910036229	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30404	1.0785889938798128	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28491	1.0107248725374867	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24211	0.8588908739252779	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19429	0.6892483081861229	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19161	0.6797409456561996	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16587	0.5884276950889505	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15819	0.5611827158987225	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15778	0.5597282313325774	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15293	0.5425227431720818	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14943	0.5301064115098685	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10895	0.38650266702804104	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10493	0.372241623233156	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10056	0.3567389462720496	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9950	0.352978571540065	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8666	0.3074283719564024	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6939	0.24616264401170965	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6834	0.24243774451304564	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6630	0.23520079691564133	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6436	0.22831860165144305	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5965	0.21160976675743595	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5304	0.18816063753251305	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5041	0.17883065116919275	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5034	0.17858232453594847	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4530	0.16070280694236128	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4115	0.14598058511430828	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4114	0.1459451098809877	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4003	0.14200735898240002	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3544	0.12572422688824025	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3257	0.1155428349252253	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	3110	0.1103279756270957	No Hit
TAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2819	0.10000468273079832	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0024832663324426685	0.0	0.0	0.0	0.0
2	0.0054986611646944804	0.0	0.0	0.0	0.0
3	0.008549531230266902	0.0	0.0	0.0	0.0
4	0.013835340995037724	0.0	0.0	0.0	0.0
5	0.02355555492488474	0.0	0.0	0.0	0.0
6	0.043670012217670355	0.0	0.0	0.0	0.0
7	0.06701271574263144	0.0	0.0	0.0	0.0
8	0.12948460162022485	0.0	0.0	0.0	0.0
9	0.34804751410850027	0.0	0.0	3.547523332060955E-5	0.0
10-11	0.8017934858957567	0.0	0.0	3.547523332060955E-5	0.0
12-13	1.2961940750684318	0.0	0.0	3.547523332060955E-5	0.0
14-15	1.7911800055908969	0.0	0.0	3.547523332060955E-5	0.0
16-17	2.286467475596587	0.0	0.0	3.547523332060955E-5	0.0
18-19	2.830639817118077	0.0	0.0	3.547523332060955E-5	0.0
20-21	3.5343620204990085	0.0	0.0	3.547523332060955E-5	0.0
22-23	4.330443993830148	0.0	0.0	7.09504666412191E-5	0.0
24-25	5.094474093856116	0.0	0.0	7.09504666412191E-5	0.0
26-27	5.751493152570465	0.0	0.0	7.09504666412191E-5	0.0
28-29	6.325446952464606	0.0	0.0	7.09504666412191E-5	0.0
30-31	6.9021500829410956	0.0	0.0	8.868808330152387E-5	0.0
32-33	7.551932194058041	0.0	1.7737616660304776E-5	1.0642569996182865E-4	0.0
34-35	8.164358884488383	0.0	3.547523332060955E-5	1.0642569996182865E-4	0.0
36-37	8.648578081698044	0.0	3.547523332060955E-5	1.0642569996182865E-4	0.0
38-39	9.019861873631545	0.0	3.547523332060955E-5	1.0642569996182865E-4	0.0
40-41	9.342207581199261	0.0	3.547523332060955E-5	1.2416331662213343E-4	0.0
42-43	9.621734682149004	0.0	3.547523332060955E-5	1.419009332824382E-4	0.0
44-45	9.856421088181497	0.0	5.3212849980914325E-5	1.419009332824382E-4	0.0
46-47	10.062886946107444	0.0	7.09504666412191E-5	1.419009332824382E-4	0.0
48-49	10.264794236551694	0.0	7.09504666412191E-5	1.419009332824382E-4	0.0
50-51	10.470763441211153	0.0	7.09504666412191E-5	1.419009332824382E-4	0.0
52-53	10.68705593876691	0.0	7.09504666412191E-5	1.419009332824382E-4	0.0
54-55	10.893450846226216	0.0	7.09504666412191E-5	1.419009332824382E-4	0.0
56-57	11.10392540551739	0.0	7.09504666412191E-5	1.419009332824382E-4	0.0
58-59	11.330966898769294	3.547523332060955E-5	7.09504666412191E-5	1.419009332824382E-4	0.0
60-61	11.571684094466288	3.547523332060955E-5	7.09504666412191E-5	1.419009332824382E-4	0.0
62-63	11.79464593588632	3.547523332060955E-5	7.09504666412191E-5	1.419009332824382E-4	0.0
64-65	11.995488969330951	3.547523332060955E-5	7.09504666412191E-5	1.419009332824382E-4	0.0
66-67	12.185920021795983	3.547523332060955E-5	7.09504666412191E-5	1.419009332824382E-4	0.0
68-69	12.363491302182293	3.547523332060955E-5	7.09504666412191E-5	2.128513999236573E-4	0.0
70-71	12.535280119537347	3.547523332060955E-5	7.09504666412191E-5	2.128513999236573E-4	0.0
72-73	12.705525764242951	3.547523332060955E-5	7.09504666412191E-5	2.128513999236573E-4	0.0
74-75	12.876924354031477	3.547523332060955E-5	7.09504666412191E-5	2.128513999236573E-4	0.0
76-77	13.048039141953437	3.547523332060955E-5	7.09504666412191E-5	2.128513999236573E-4	0.0
78-79	13.215588668926674	3.547523332060955E-5	7.09504666412191E-5	2.128513999236573E-4	0.0
80-81	13.380246964384284	3.547523332060955E-5	7.09504666412191E-5	2.128513999236573E-4	0.0
82-83	13.545171324091799	3.547523332060955E-5	7.09504666412191E-5	2.3058901658396208E-4	0.0
84-85	13.711284104115553	3.547523332060955E-5	7.09504666412191E-5	2.4832663324426686E-4	0.0
86-87	13.881813550687722	3.547523332060955E-5	7.09504666412191E-5	2.4832663324426686E-4	0.0
88	14.011511003707872	3.547523332060955E-5	7.09504666412191E-5	2.4832663324426686E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAAA	2150	0.0	50.504967	1
TGAAAAA	3145	0.0	47.679382	1
GAAAAAA	29550	0.0	43.045807	1
TAACGAC	235	0.0	37.997387	6
AGGCGAC	50	0.0017021304	37.60142	2
GCTACGT	50	0.0017021304	37.60142	2
CGTAACG	50	0.0017027246	37.598747	4
CTACCCG	685	0.0	37.049866	5
TACCCGA	710	0.0	36.405945	6
TGCCGAT	65	1.4145822E-4	36.151356	6
AGTTGCC	120	4.2109605E-9	35.25008	3
GTACGAC	80	1.1597911E-5	35.247574	6
CTAACGA	190	0.0	34.63043	5
CCCGACG	765	0.0	34.40592	8
CTAAAAA	6945	0.0	34.31597	1
CTCACGA	345	0.0	34.05684	5
TTACGTC	85	1.7556536E-5	33.176544	3
ACCCGAC	880	0.0	33.112537	7
TTACGAC	130	9.204086E-9	32.53622	6
TACGACG	420	0.0	32.4513	7
>>END_MODULE