##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248475_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2818868 Sequences flagged as poor quality 0 Sequence length 100 %GC 20 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78551815835293 34.0 31.0 34.0 31.0 34.0 2 32.95510254470943 34.0 33.0 34.0 31.0 34.0 3 33.05040498526359 34.0 33.0 34.0 31.0 34.0 4 36.43963534298165 37.0 37.0 37.0 35.0 37.0 5 36.371346228344144 37.0 37.0 37.0 35.0 37.0 6 36.302266016003585 37.0 37.0 37.0 35.0 37.0 7 36.344193130008215 37.0 37.0 37.0 35.0 37.0 8 36.16248330890272 37.0 37.0 37.0 35.0 37.0 9 37.93860727072002 39.0 39.0 39.0 35.0 39.0 10-11 38.14130548149115 39.0 39.0 39.0 37.0 39.0 12-13 38.21177171119754 39.0 39.0 39.0 37.0 39.0 14-15 39.88586162956194 41.0 40.0 41.0 38.0 41.0 16-17 39.838624937386214 41.0 40.0 41.0 38.0 41.0 18-19 39.50498019062971 41.0 39.5 41.0 36.5 41.0 20-21 38.74142740986807 40.5 37.5 41.0 35.0 41.0 22-23 37.69816961986159 38.0 35.0 41.0 35.0 41.0 24-25 36.771935046266805 36.0 35.0 41.0 34.5 41.0 26-27 36.500984260348474 35.0 35.0 41.0 34.0 41.0 28-29 36.33906855517888 35.0 35.0 40.5 34.0 41.0 30-31 36.25102115459113 35.0 35.0 40.0 33.0 41.0 32-33 36.19380616616316 35.0 35.0 40.0 33.0 41.0 34-35 36.137980920000516 35.0 35.0 40.0 33.0 41.0 36-37 36.082977457617744 35.0 35.0 40.0 33.0 41.0 38-39 36.01301550835299 35.0 35.0 40.0 33.0 41.0 40-41 35.941228358333916 35.0 35.0 40.0 33.0 41.0 42-43 35.85401019132503 35.0 35.0 40.0 33.0 41.0 44-45 35.77810755948842 35.0 35.0 40.0 33.0 41.0 46-47 35.69515635354334 35.0 35.0 40.0 33.0 41.0 48-49 35.594250245133864 35.0 35.0 40.0 33.0 41.0 50-51 35.44584705633609 35.0 35.0 39.0 33.0 41.0 52-53 35.23438344753993 35.0 35.0 39.0 33.0 41.0 54-55 35.118525592542824 35.0 35.0 38.5 33.0 41.0 56-57 34.975292919001525 35.0 35.0 37.5 32.5 41.0 58-59 34.83435708944158 35.0 35.0 37.0 32.0 41.0 60-61 34.62951546507321 35.0 35.0 36.5 31.0 41.0 62-63 34.40064823893847 35.0 35.0 35.5 31.0 40.0 64-65 34.240356944702626 35.0 35.0 35.0 31.0 40.0 66-67 34.08544990400402 35.0 35.0 35.0 30.0 39.0 68-69 33.93278578493211 35.0 35.0 35.0 30.0 39.0 70-71 33.814839680325576 35.0 35.0 35.0 30.0 39.0 72-73 33.67728854277675 35.0 35.0 35.0 29.0 37.5 74-75 33.53056209088187 35.0 35.0 35.0 29.0 37.0 76-77 33.399098325994686 35.0 35.0 35.0 29.0 36.5 78-79 33.26487299156966 35.0 34.5 35.0 29.0 36.0 80-81 33.138123885190794 35.0 34.0 35.0 29.0 36.0 82-83 33.02321765332751 35.0 34.0 35.0 29.0 35.5 84-85 32.89340455104673 35.0 34.0 35.0 27.5 35.0 86-87 32.77968940014219 35.0 34.0 35.0 27.0 35.0 88-89 32.66268817837515 35.0 34.0 35.0 27.0 35.0 90-91 32.54617332205694 35.0 34.0 35.0 27.0 35.0 92-93 32.384662034547205 35.0 33.5 35.0 25.5 35.0 94-95 32.1771454002103 35.0 33.0 35.0 24.0 35.0 96-97 32.006204440931604 35.0 33.0 35.0 24.0 35.0 98-99 31.814586919288168 35.0 33.0 35.0 23.5 35.0 100 31.474013682087985 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22832235305936166 1101 2 0.18651205965349504 1101 3 0.16043604978870718 1101 4 0.09813115757047797 1101 5 0.11537119035159549 1101 6 0.045585158837255335 1101 7 0.07534031347935866 1101 8 0.022972279952355734 1101 9 -0.04215282829888878 1101 10-11 0.07465734797950319 1101 12-13 0.10828920644644313 1101 14-15 0.208550132504854 1101 16-17 0.1969126060360935 1101 18-19 0.25266396267856805 1101 20-21 0.26175212895645217 1101 22-23 0.2388416310825079 1101 24-25 0.21069513221988245 1101 26-27 0.2248288889422696 1101 28-29 0.21122974698256058 1101 30-31 0.2393473253468983 1101 32-33 0.2214442371410854 1101 34-35 0.2261685469792596 1101 36-37 0.18913726316337431 1101 38-39 0.15830309340112336 1101 40-41 0.09851428066191659 1101 42-43 0.1328408899376754 1101 44-45 0.16931277948215495 1101 46-47 0.21748741879056865 1101 48-49 0.14968087133188135 1101 50-51 0.17721634800003017 1101 52-53 0.08609981668446665 1101 54-55 0.1156373715971597 1101 56-57 0.14898578749261304 1101 58-59 0.15731768283060887 1101 60-61 0.18831448447554777 1101 62-63 0.20001787726421583 1101 64-65 0.1807173362403418 1101 66-67 0.16362860686532343 1101 68-69 0.15347264523613546 1101 70-71 0.14526211383157772 1101 72-73 0.12987298186457963 1101 74-75 0.1421432051288889 1101 76-77 0.19369822239443835 1101 78-79 0.259109618520057 1101 80-81 0.2189962606514939 1101 82-83 0.13989005759043494 1101 84-85 0.17496382050129 1101 86-87 0.127755353502927 1101 88-89 0.24631433066434028 1101 90-91 0.22003182308782243 1101 92-93 0.2303397208218101 1101 94-95 0.244290567927969 1101 96-97 0.22085679897254806 1101 98-99 0.2254156049355842 1101 100 0.181179511020229 1102 1 0.16744088404874446 1102 2 0.1542069544977167 1102 3 0.1551679543278155 1102 4 0.10583288191558893 1102 5 0.08579357117249486 1102 6 -0.05766134683599233 1102 7 0.02799712354956796 1102 8 0.008794913627774292 1102 9 -0.049846163575288926 1102 10-11 0.0336612075902778 1102 12-13 0.10754681381374098 1102 14-15 0.16673222291376533 1102 16-17 0.2027392936290937 1102 18-19 0.22785540921606895 1102 20-21 0.16556035069120156 1102 22-23 0.14741975744072988 1102 24-25 0.1283195908453152 1102 26-27 0.11595691348117754 1102 28-29 0.14336825322339308 1102 30-31 0.09800985372591953 1102 32-33 0.1345552170098756 1102 34-35 0.1185743065782745 1102 36-37 0.11638909693389365 1102 38-39 0.17138033323453072 1102 40-41 0.09427855997540036 1102 42-43 0.03535228149402059 1102 44-45 0.053355756121874265 1102 46-47 0.08096810891210282 1102 48-49 0.09027168387949303 1102 50-51 0.028696275595173404 1102 52-53 0.07096645605869867 1102 54-55 0.13942936967198705 1102 56-57 0.20501218285839684 1102 58-59 0.17809456971592397 1102 60-61 0.15872360257829143 1102 62-63 0.14663527692776768 1102 64-65 0.14276347164406644 1102 66-67 0.13055466353644363 1102 68-69 0.1008805348596411 1102 70-71 0.08830477152689298 1102 72-73 0.07593342726180907 1102 74-75 0.12627332421851634 1102 76-77 0.16647489547834482 1102 78-79 0.1765349765814932 1102 80-81 0.1320148808686099 1102 82-83 0.17917087311656843 1102 84-85 0.1311497120371854 1102 86-87 0.08572008713641566 1102 88-89 0.06560759876426658 1102 90-91 0.07256785453739667 1102 92-93 0.13086374200125306 1102 94-95 0.10184553949781616 1102 96-97 0.17166605647764754 1102 98-99 0.18490734881363835 1102 100 0.11814596625040608 1103 1 0.14292830411324786 1103 2 0.18293129590383472 1103 3 0.13677434310795178 1103 4 0.0860520519847583 1103 5 0.049861635195462384 1103 6 0.005447199969388805 1103 7 0.04964117289982539 1103 8 -0.04343349213690573 1103 9 -0.14334155854518116 1103 10-11 -0.0204658117713592 1103 12-13 0.06515886333179566 1103 14-15 0.12201699601814653 1103 16-17 0.10869346821939985 1103 18-19 0.09774612131778326 1103 20-21 0.06321378954870482 1103 22-23 0.00540244080296759 1103 24-25 -0.024234849186747454 1103 26-27 -0.027124413308165174 1103 28-29 0.05504594306436417 1103 30-31 -0.016698305986594164 1103 32-33 -0.004381058322451281 1103 34-35 0.028255793937745466 1103 36-37 0.02460398357931126 1103 38-39 0.04350466555177235 1103 40-41 -0.007138576283153952 1103 42-43 -0.005242800958384919 1103 44-45 0.026439430870652814 1103 46-47 0.0607258437779592 1103 48-49 0.07512807382487097 1103 50-51 0.10328797535466805 1103 52-53 0.04141960057647509 1103 54-55 0.03944518781058548 1103 56-57 0.05504705267773602 1103 58-59 0.03658063693631419 1103 60-61 0.07566526598229473 1103 62-63 0.046235832514391006 1103 64-65 0.016896678432289036 1103 66-67 -0.02621649023800643 1103 68-69 0.03547500789131419 1103 70-71 0.03492030161805815 1103 72-73 0.005818872476865522 1103 74-75 0.04361583032437011 1103 76-77 0.05876851974890229 1103 78-79 -0.003909492721419383 1103 80-81 -0.05674124829596394 1103 82-83 -0.009171013081392232 1103 84-85 -0.03723160589632357 1103 86-87 0.017417335537707856 1103 88-89 0.006245736849926686 1103 90-91 -0.013868368359112537 1103 92-93 -0.013541589206688798 1103 94-95 -0.08119712461464701 1103 96-97 -0.14859662949472252 1103 98-99 -0.05817607540844705 1103 100 -0.1114792225185397 1104 1 -0.2181811445761639 1104 2 -0.22518042670393612 1104 3 -0.17741580785794042 1104 4 -0.27518216984128685 1104 5 -0.06488757903575504 1104 6 -0.03511182486197839 1104 7 0.03711979743590632 1104 8 0.01198383076691556 1104 9 0.03985395697925753 1104 10-11 0.10514611192842693 1104 12-13 0.12199608119557581 1104 14-15 0.16636207568764405 1104 16-17 0.16388732504352532 1104 18-19 0.1309409230685077 1104 20-21 0.11179998911818956 1104 22-23 0.03960688920550126 1104 24-25 -0.04131363552984624 1104 26-27 -0.0034877622905327144 1104 28-29 -0.03833048389970628 1104 30-31 -0.024611311245735124 1104 32-33 -0.08340775098878339 1104 34-35 -0.06441732570088732 1104 36-37 -0.04427809715893005 1104 38-39 -0.07528419908405937 1104 40-41 -0.0715460164407773 1104 42-43 -0.11097423920419658 1104 44-45 -0.10951160809563021 1104 46-47 -0.07046360384627803 1104 48-49 -0.05622797819385994 1104 50-51 -0.028661744228337227 1104 52-53 -0.038029934386223374 1104 54-55 -0.004356257087039239 1104 56-57 0.024557762166068642 1104 58-59 1.1589365189479395E-4 1104 60-61 0.026090935920692004 1104 62-63 -0.016143392168125104 1104 64-65 0.01973533592275345 1104 66-67 -0.021332527882428565 1104 68-69 -0.050841304775616436 1104 70-71 -0.03117435548367098 1104 72-73 0.03711428118232618 1104 74-75 0.06683959069845713 1104 76-77 -0.038373303794706715 1104 78-79 -0.014150258205290811 1104 80-81 -0.018913128576343752 1104 82-83 0.01250293178947004 1104 84-85 0.01783905632920124 1104 86-87 0.07523002127329192 1104 88-89 0.018835881666369403 1104 90-91 -0.020649939325650735 1104 92-93 0.07464710977874489 1104 94-95 0.1186072533439102 1104 96-97 0.160547724651245 1104 98-99 0.16032375205048766 1104 100 0.1535352135549246 1105 1 0.16923865752846723 1105 2 0.13777098242201902 1105 3 0.1465370542770188 1105 4 0.07406617975744467 1105 5 0.1274199436180865 1105 6 0.0050907973049874045 1105 7 0.015009563474542631 1105 8 -0.04301450040470911 1105 9 0.026604591188380766 1105 10-11 0.0853480091571086 1105 12-13 0.12207471206716747 1105 14-15 0.16070864387522477 1105 16-17 0.18977951214587563 1105 18-19 0.16407718698081908 1105 20-21 0.16593398801390435 1105 22-23 0.16549120682793728 1105 24-25 0.10913058734175252 1105 26-27 0.11387728396007901 1105 28-29 0.14881041514266968 1105 30-31 0.12917366886417625 1105 32-33 0.13647143107882442 1105 34-35 0.11148140438496768 1105 36-37 0.12566511153424642 1105 38-39 0.1217589157570913 1105 40-41 0.16443232516510164 1105 42-43 0.14095763500865388 1105 44-45 0.1672454386848159 1105 46-47 0.22906666939088893 1105 48-49 0.19581333996384842 1105 50-51 0.20119949913286206 1105 52-53 -0.4460247825938026 1105 54-55 -0.3217077698778894 1105 56-57 -0.14957787838881842 1105 58-59 -0.09826833143543467 1105 60-61 -0.08097084512225194 1105 62-63 -0.05917132383547141 1105 64-65 0.024672811274697892 1105 66-67 -0.03446624875729043 1105 68-69 -0.030855926282079338 1105 70-71 -0.02173986551579077 1105 72-73 -0.0799687806986995 1105 74-75 -0.08227816772589591 1105 76-77 -0.01935818550734325 1105 78-79 -0.07038312602469432 1105 80-81 -0.024011669359971677 1105 82-83 -0.0021177116527013595 1105 84-85 0.0035558224319558462 1105 86-87 -0.03978226783694794 1105 88-89 -0.06639637123403475 1105 90-91 -0.019307141392474136 1105 92-93 -0.027257700710947574 1105 94-95 -0.0060650301240912086 1105 96-97 -0.07379260165152601 1105 98-99 -0.14823855533983732 1105 100 -0.16049264261399543 1106 1 0.1352841239628404 1106 2 0.11182646110067651 1106 3 0.1127596417021266 1106 4 0.07902508570018085 1106 5 0.10731017678362775 1106 6 0.052072968189222024 1106 7 0.09467968120007697 1106 8 -0.01818106550233267 1106 9 0.04555924731138106 1106 10-11 0.06581639992588606 1106 12-13 0.13208243950488452 1106 14-15 0.14458458113043093 1106 16-17 0.14609283960007247 1106 18-19 0.12572434956096856 1106 20-21 0.13279236812832096 1106 22-23 0.13850636316927734 1106 24-25 0.1475213964666935 1106 26-27 0.1576173758594379 1106 28-29 0.22835993280565248 1106 30-31 0.23041987553299492 1106 32-33 0.22794403402686925 1106 34-35 0.19682129089382983 1106 36-37 0.18106546790818356 1106 38-39 0.24093671060256838 1106 40-41 0.24875670193378596 1106 42-43 0.18916676473150318 1106 44-45 0.2522614063740036 1106 46-47 0.3038032659059553 1106 48-49 0.3233287395830615 1106 50-51 0.2771832297779113 1106 52-53 0.28163663795508853 1106 54-55 0.2867889245963582 1106 56-57 0.26530855971407874 1106 58-59 0.29624660306804174 1106 60-61 0.26615145671107143 1106 62-63 0.2385350647277562 1106 64-65 0.21959741259738763 1106 66-67 0.19392599913694397 1106 68-69 0.2449399754116186 1106 70-71 0.2311704202632967 1106 72-73 0.23125258386285452 1106 74-75 0.18481992496228372 1106 76-77 0.20362624115514194 1106 78-79 0.25347402351739845 1106 80-81 0.22911693113908882 1106 82-83 0.27081034765470235 1106 84-85 0.2735202518876605 1106 86-87 0.23887798320750875 1106 88-89 0.21929999761702135 1106 90-91 0.2519460073833102 1106 92-93 0.30754653181223546 1106 94-95 0.3252704374539519 1106 96-97 0.31020531481300395 1106 98-99 0.24543925513395237 1106 100 0.2942552930170699 1107 1 0.07252180189264124 1107 2 0.09092107879862965 1107 3 0.06813873016702843 1107 4 0.023920660654233927 1107 5 0.028310367029973804 1107 6 0.031131626467384876 1107 7 0.03482902137099586 1107 8 -0.07179416701338681 1107 9 -0.1169435890170405 1107 10-11 -0.06687035657589746 1107 12-13 0.016306592548176013 1107 14-15 0.051222368958292464 1107 16-17 0.11803465585782646 1107 18-19 0.12590414665994132 1107 20-21 0.06808677799717344 1107 22-23 0.024278289031457234 1107 24-25 0.12297127011544973 1107 26-27 0.17445461529447215 1107 28-29 0.13653221298486784 1107 30-31 0.11556709403976129 1107 32-33 0.08815330734244498 1107 34-35 0.11245033468674848 1107 36-37 0.13793328219015422 1107 38-39 0.12874253277120573 1107 40-41 0.10723344339857732 1107 42-43 0.17077042759535743 1107 44-45 0.11564722158543361 1107 46-47 0.14553649009232572 1107 48-49 0.09421957174649975 1107 50-51 0.08588688043541737 1107 52-53 0.14031620572519898 1107 54-55 0.12425914789092474 1107 56-57 0.11016966966577968 1107 58-59 0.12954027985509242 1107 60-61 0.1612358799135194 1107 62-63 0.11341651101561467 1107 64-65 0.0886710331464684 1107 66-67 0.08536556153882913 1107 68-69 0.15358457322972896 1107 70-71 0.08019835717156099 1107 72-73 0.08209399050429056 1107 74-75 0.12396672117937158 1107 76-77 0.12258269879269079 1107 78-79 0.09134741845614514 1107 80-81 0.1630535518444418 1107 82-83 0.17140061919560878 1107 84-85 0.1412257799654668 1107 86-87 0.12457480875546167 1107 88-89 0.16456103352501117 1107 90-91 0.14778800099222167 1107 92-93 0.20039230600540492 1107 94-95 0.17720840208423994 1107 96-97 0.13391157398784514 1107 98-99 0.17325775416558997 1107 100 0.12923541405437433 1108 1 0.03812969751349726 1108 2 0.010605348893598432 1108 3 0.039499559621972935 1108 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2214 36-37 -0.24101036251699526 2214 38-39 -0.2121121811322766 2214 40-41 -0.21470792817407158 2214 42-43 -0.18522639451524014 2214 44-45 -0.17179264146162154 2214 46-47 -0.1528535353283189 2214 48-49 -0.18713738994520668 2214 50-51 -0.19679630071910736 2214 52-53 -0.2094206332941866 2214 54-55 -0.21142761737785065 2214 56-57 -0.204137225073886 2214 58-59 -0.1969512390887047 2214 60-61 -0.1986295005523715 2214 62-63 -0.20632226673164666 2214 64-65 -0.188009026970974 2214 66-67 -0.16410315903262784 2214 68-69 -0.15918801617840472 2214 70-71 -0.1785185836736929 2214 72-73 -0.15385558212918227 2214 74-75 -0.16323081228786407 2214 76-77 -0.13631848736291374 2214 78-79 -0.22267167853833314 2214 80-81 -0.21136917013773626 2214 82-83 -0.1643242453386904 2214 84-85 -0.18191355114740304 2214 86-87 -0.24180924197030862 2214 88-89 -0.21625684409220725 2214 90-91 -0.07934391869562063 2214 92-93 -0.1368368635405659 2214 94-95 -0.20715197718003253 2214 96-97 -0.24366558466748955 2214 98-99 -0.2541407645623437 2214 100 -0.30718761153560337 2215 1 -0.2472573336993591 2215 2 -0.19512113436625356 2215 3 -0.24085210789498745 2215 4 -0.1642899847550794 2215 5 -0.196202718653268 2215 6 -0.15995389369915358 2215 7 -0.18178337171322312 2215 8 0.02330981272638155 2215 9 -0.0067409387956303135 2215 10-11 -0.09311584552019525 2215 12-13 -0.1758136762930178 2215 14-15 -0.2631088682462277 2215 16-17 -0.31150276019888423 2215 18-19 -0.3580100012308236 2215 20-21 -0.35666210791870157 2215 22-23 -0.3074607079120639 2215 24-25 -0.2923023284669526 2215 26-27 -0.38874864410453114 2215 28-29 -0.4342758195083718 2215 30-31 -0.39705710899546176 2215 32-33 -0.3440618482898614 2215 34-35 -0.42596866807707556 2215 36-37 -0.4072648803027974 2215 38-39 -0.40347607689032117 2215 40-41 -0.38400600692620657 2215 42-43 -0.4634268890938955 2215 44-45 -0.4820456374669533 2215 46-47 -0.43400247959311145 2215 48-49 -0.4123609014536811 2215 50-51 -0.4681487831235245 2215 52-53 -0.36454713129684535 2215 54-55 -0.36741011690229897 2215 56-57 -0.40215891044577035 2215 58-59 -0.3964947049437626 2215 60-61 -0.32636394985804884 2215 62-63 -0.3194095788868765 2215 64-65 -0.2727531776272514 2215 66-67 -0.28384826080169745 2215 68-69 -0.3460816817671457 2215 70-71 -0.32717751462290323 2215 72-73 -0.3421759169208798 2215 74-75 -0.3734267415250656 2215 76-77 -0.3454681925179486 2215 78-79 -0.33756610501731643 2215 80-81 -0.33567961335630514 2215 82-83 -0.3710010572085807 2215 84-85 -0.37422855970741864 2215 86-87 -0.454097619371872 2215 88-89 -0.47324752318674257 2215 90-91 -0.4197471905236583 2215 92-93 -0.38060707468761024 2215 94-95 -0.40764655583706144 2215 96-97 -0.339632866387106 2215 98-99 -0.38463344099376684 2215 100 -0.4692572329927067 2216 1 -0.2808861628731947 2216 2 -0.2253597188080647 2216 3 -0.28801268974926586 2216 4 -0.15217923239336528 2216 5 -0.17277762917937878 2216 6 -0.10319047387544344 2216 7 -0.13705940228489055 2216 8 -0.015126230291627962 2216 9 -0.04615253380550399 2216 10-11 -0.1571075966637352 2216 12-13 -0.26606747853966795 2216 14-15 -0.3850749128893227 2216 16-17 -0.3814003549542804 2216 18-19 -0.3228675799621783 2216 20-21 -0.2790617592193385 2216 22-23 -0.220311060705626 2216 24-25 -0.12938489387642704 2216 26-27 -0.20563499053383083 2216 28-29 -0.24911658054978147 2216 30-31 -0.2571480378988369 2216 32-33 -0.22882716278066084 2216 34-35 -0.2582692146834873 2216 36-37 -0.2553459629841086 2216 38-39 -0.251259228210472 2216 40-41 -0.28312695194181714 2216 42-43 -0.23995506281875123 2216 44-45 -0.2966833264770017 2216 46-47 -0.30513866256957556 2216 48-49 -0.2841376683504109 2216 50-51 -0.2992564705084959 2216 52-53 -0.26798146735082184 2216 54-55 -0.2772220737129629 2216 56-57 -0.2939101799609034 2216 58-59 -0.25752326730211905 2216 60-61 -0.25195707261963207 2216 62-63 -0.296060452461937 2216 64-65 -0.2915111376855464 2216 66-67 -0.3280860919117785 2216 68-69 -0.35151587420939023 2216 70-71 -0.2496790798648334 2216 72-73 -0.290281312816866 2216 74-75 -0.278200194014147 2216 76-77 -0.3534716492775871 2216 78-79 -0.31009343218877916 2216 80-81 -0.3810623071345134 2216 82-83 -0.38449854463310373 2216 84-85 -0.39452114971985 2216 86-87 -0.4210816595730762 2216 88-89 -0.37847713189515986 2216 90-91 -0.42805412155348677 2216 92-93 -0.4864040757218646 2216 94-95 -0.5351844838220714 2216 96-97 -0.4887511029357867 2216 98-99 -0.5094942698854261 2216 100 -0.4579056072196934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 43.0 8 207.0 9 416.0 10 803.0 11 1322.0 12 1993.0 13 2749.0 14 3318.0 15 4083.0 16 5119.0 17 6134.0 18 7214.0 19 8726.0 20 10009.0 21 11941.0 22 13300.0 23 14925.0 24 16773.0 25 18947.0 26 21219.0 27 24560.0 28 28435.0 29 34138.0 30 38605.0 31 46474.0 32 60204.0 33 89254.0 34 138348.0 35 1243289.0 36 208452.0 37 336918.0 38 306387.0 39 112987.0 40 1572.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.93965892819384 21.227547714432898 33.2110050080819 25.621788349291364 2 13.144526108837137 22.882526019439105 40.15277130351554 23.820176568208215 3 9.190149514024434 16.95302295320175 50.9377884581956 22.91903907457822 4 9.412897043678042 13.152760862215338 59.13420859469429 18.300133499412325 5 7.843947036681842 12.233832503275703 62.120242868582665 17.801977591459792 6 6.916036660454414 9.676639976328866 57.16823427609865 26.239089087118074 7 8.709879284876058 9.461138300906605 57.7814924288757 24.047489985341635 8 4.7622662714252675 9.647808978639652 59.95768514169517 25.632239608239903 9 5.901269587650078 8.53778892803778 62.7045679329433 22.856373551368847 10-11 9.298518585666104 13.778081085122828 66.81349123212537 10.10990909708569 12-13 8.64420510468114 15.355941770157552 65.64366922438201 10.356183900779307 14-15 9.418995893871777 14.260676084148932 65.54738650056025 10.772941521419039 16-17 10.430441815209306 14.799389611294922 66.10322366191006 8.666944911585697 18-19 10.555501652880688 13.502767867864618 65.48367934416191 10.458051135092775 20-21 9.366616693497978 13.148128702312933 66.4876158541474 10.997638750041684 22-23 9.549371981498943 13.396291274576372 67.3250874642498 9.729249279674876 24-25 9.381715380586199 13.51429684849046 66.97955961204822 10.124428158875117 26-27 10.485989530950391 13.122834838414423 66.18204297241542 10.209132658219765 28-29 10.024187451686291 13.983135478464902 66.65631362376486 9.336363446083942 30-31 10.060456502200314 13.576522804799978 66.65202097272832 9.710999720271385 32-33 10.204948623600647 13.779407862341616 66.6179344147376 9.397709099320137 34-35 10.153696553960353 14.064348636175453 66.70710348123191 9.07485132863228 36-37 10.237826932433624 13.511128975067896 66.6305997636258 9.620444328872685 38-39 10.211929713528868 13.48168419192961 66.48117635810958 9.825209736431942 40-41 10.311898583295049 13.738951670264452 66.46464091043735 9.484508836003142 42-43 10.393924085838712 14.013639517707107 66.19265251157557 9.39978388487861 44-45 10.461647726012945 13.705649665102612 66.06468918070624 9.76801342817821 46-47 10.523171560184684 13.715928219336506 66.22356870584437 9.537331514634436 48-49 10.142313152791878 13.377280270634802 66.57496273308232 9.905443843491007 50-51 10.01583599410564 13.4069566590577 66.54634268381508 10.030864663021578 52-53 9.892298653737608 13.592546201636468 66.44585912216047 10.069296022465446 54-55 9.990079275334788 13.469215155915727 66.67308773287229 9.867617835877192 56-57 9.904193590213637 13.654058562295452 66.61352464461274 9.828223202878183 58-59 9.96099126737939 13.748148184937437 66.73581410330999 9.555046444373179 60-61 9.75362544554213 13.48716828020294 66.98725714916185 9.771949125093082 62-63 9.500878654808405 13.314733691134299 67.15672413077392 10.027663523283376 64-65 9.734269143457691 13.27981910162512 67.09318267457381 9.892729080343376 66-67 9.628799753047652 13.360139726773484 67.27035161437114 9.740708905807727 68-69 9.775687965402188 13.165871189252778 67.3467447105616 9.711696134783436 70-71 9.829589090380743 13.112168332775392 67.22863574586019 9.82960683098368 72-73 9.702641806129177 12.942130471874897 67.48134049348414 9.873887228511787 74-75 9.512084006874735 12.764632583461182 67.69816664277634 10.025116766887734 76-77 9.520263205419369 12.652497815314126 67.838301667954 9.988937311312505 78-79 9.79166337732156 12.592322677392682 67.75448931298428 9.861524632301482 80-81 9.767482951996291 12.480279417131152 67.84669951109453 9.905538119778026 82-83 9.92025681877732 12.617769365852691 67.61349313310286 9.848480682267127 84-85 9.734053131186299 12.895743498304846 67.49651806569968 9.873685304809177 86-87 9.580781385959108 13.202312918050001 67.43514986158503 9.781755834405853 88-89 9.506396948382168 13.621390701421163 67.2236511380503 9.64856121214637 90-91 9.495077323652067 14.004593350799208 67.03288944896997 9.467439876578753 92-93 9.373728321371793 14.381006713576197 66.78848172703138 9.456783238020632 94-95 9.454221238895666 14.787405795993367 66.47679365688144 9.281579308229531 96-97 9.20840024500626 15.268704210996297 66.45926043658015 9.063635107417294 98-99 9.118499893742591 15.677083203985486 66.24856712150986 8.955849780762051 100 9.173238780515582 16.04728728897776 65.94835523013013 8.831118700376535 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 109318.0 1 294859.5 2 525390.0 3 409961.0 4 150906.0 5 35024.5 6 13526.5 7 7740.0 8 5670.0 9 4895.5 10 4524.5 11 4383.0 12 4498.5 13 4981.5 14 5645.0 15 6447.0 16 7479.5 17 8307.0 18 8891.0 19 9352.0 20 9377.0 21 9018.5 22 9073.0 23 9647.5 24 10386.0 25 11417.0 26 12711.0 27 14089.0 28 15641.5 29 17362.5 30 19060.5 31 20916.0 32 22862.5 33 25070.0 34 27367.5 35 29170.0 36 30270.5 37 31034.0 38 31601.0 39 31615.0 40 31219.0 41 31008.5 42 31426.5 43 32668.5 44 35603.5 45 40358.0 46 45866.0 47 51456.0 48 56577.5 49 60701.0 50 64211.5 51 65903.5 52 63907.0 53 58269.0 54 49639.5 55 39453.5 56 29622.0 57 21000.0 58 14313.5 59 9890.5 60 7147.5 61 5344.5 62 4004.5 63 3201.0 64 2553.5 65 1964.0 66 1590.5 67 1262.0 68 979.5 69 745.0 70 562.0 71 411.5 72 293.0 73 222.0 74 155.5 75 109.5 76 82.0 77 64.5 78 50.0 79 37.0 80 30.5 81 27.5 82 21.0 83 16.0 84 14.0 85 15.0 86 10.0 87 4.0 88 8.0 89 7.5 90 2.5 91 2.0 92 1.5 93 0.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00794645226381654 2 4.966532664885337E-4 3 7.09504666412191E-5 4 0.004576305098358632 5 0.013799865761717116 6 0.00869143216354934 7 0.0 8 0.0 9 0.0 10-11 0.011050535179369875 12-13 0.005019745514866251 14-15 0.0011529450829198104 16-17 3.015394832251812E-4 18-19 5.3212849980914325E-5 20-21 7.804551330534102E-4 22-23 7.09504666412191E-5 24-25 4.434404165076194E-4 26-27 0.0029444443656105926 28-29 0.001809236899351087 30-31 0.002288152549179316 32-33 0.0022704149325190113 34-35 0.0023768406324808397 36-37 0.0011529450829198104 38-39 0.0011529450829198104 40-41 1.0642569996182865E-4 42-43 0.0 44-45 6.917670497518861E-4 46-47 1.0642569996182865E-4 48-49 4.966532664885337E-4 50-51 0.03265495227162109 52-53 0.036521752703567535 54-55 7.627175163931052E-4 56-57 2.3058901658396205E-4 58-59 4.257027998473146E-4 60-61 0.003937750898587659 62-63 0.012593707828816391 64-65 0.01738286432709868 66-67 0.018110106610171175 68-69 0.019138888376468852 70-71 0.01683299821062923 72-73 0.014136880478262906 74-75 0.015325300794503326 76-77 0.013657964828434677 78-79 0.015059236544598753 80-81 0.0152366127112018 82-83 0.013569276745133151 84-85 0.01388855384501864 86-87 0.012434069278873647 88-89 0.010553881912881341 90-91 0.008106090813759282 92-93 0.008904283563472996 94-95 0.011263386579293532 96-97 0.005835675881240271 98-99 0.008549531230266902 100 0.00947188729660275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2818868.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.32456351434265 #Duplication Level Percentage of deduplicated Percentage of total 1 89.7751232582895 38.89468029611703 2 8.088087453776623 7.008257172014066 3 1.192421570998827 1.5498343226583278 4 0.26011881825234917 0.45078137050598616 5 0.12050915576580246 0.261050328651766 6 0.07227703834097683 0.18788226829393376 7 0.053308710246409007 0.16167036220567652 8 0.03921423847242602 0.135915181229216 9 0.03264633001258002 0.12729491983261926 >10 0.2776881342392446 2.4981970052814644 >50 0.03506608068816107 1.045006073497108 >100 0.028576992444845913 2.783042322490568 >500 0.006733229982663649 2.0263112156172958 >1k 0.0098535072917029 10.821033380529968 >5k 0.0056657666927291675 18.555374246545107 >10k+ 0.0027097145052182975 13.493669534529873 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18600 0.6598393397633376 No Hit TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13705 0.48618807265895386 No Hit CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13695 0.4858333203257478 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13347 0.4734879391301757 No Hit TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13289 0.47143037559758033 No Hit CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12947 0.4592978458019319 No Hit CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12904 0.45777241076914565 No Hit CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12594 0.44677508843975666 No Hit TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12180 0.4320883418450243 No Hit CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12147 0.4309176591454442 No Hit CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11803 0.41871417888315454 No Hit CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11800 0.41860775318319265 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11403 0.40452408555491065 No Hit CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11003 0.39033399222666687 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10949 0.38841832962735395 No Hit TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10947 0.3883473791607127 No Hit TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10928 0.3876733497276212 No Hit CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10750 0.3813587581965527 No Hit CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10654 0.3779531357977741 No Hit ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10636 0.3773145815980032 No Hit ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10494 0.3722770984664766 No Hit GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10483 0.37188687089994993 No Hit TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10384 0.36837482280120953 No Hit ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10382 0.36830387233456835 No Hit GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10342 0.36688486300174394 No Hit TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10221 0.3625923597699502 No Hit ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10189 0.36145715230369074 No Hit TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10143 0.3598252915709427 No Hit ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10135 0.35954148970437777 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10116 0.3588674602712862 No Hit GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10087 0.3578386785049885 No Hit TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10028 0.35574563973907253 No Hit TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10018 0.35539088740586644 No Hit TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9974 0.3538299771397596 No Hit ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9952 0.35304952200670625 No Hit CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9917 0.35180788884048486 No Hit TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9915 0.3517369383738437 No Hit ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9915 0.3517369383738437 No Hit CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9820 0.34836679120838576 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9816 0.34822489027510334 No Hit ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9804 0.347799187475256 No Hit TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9410 0.3338219455469359 No Hit TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9174 0.325449790483272 No Hit GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9140 0.3242436325503713 No Hit GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9102 0.32289557368418814 No Hit GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8979 0.31853211998575315 No Hit AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8925 0.31661645738644023 No Hit CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8919 0.31640360598651657 No Hit GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8884 0.31516197282029523 No Hit TTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8623 0.30590293692361614 No Hit CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8575 0.3042001257242269 No Hit TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8451 0.2998011967924713 No Hit CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8438 0.2993400187593034 No Hit TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8435 0.29923359305934155 No Hit TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8423 0.2988078902594942 No Hit ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8348 0.29614724776044854 No Hit GATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8186 0.29040025996250973 No Hit TTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8185 0.29036478472918914 No Hit TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8153 0.28922957726292964 No Hit GCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8097 0.28724296419697554 No Hit ACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7811 0.2770970474672812 No Hit GTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7742 0.2746492563681592 No Hit AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7716 0.2737269003018233 No Hit TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7711 0.2735495241352202 No Hit TTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7605 0.2697891494032356 No Hit TTTTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7594 0.2693989218367089 No Hit AATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7571 0.2685829914703349 No Hit TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7570 0.2685475162370143 No Hit GCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7555 0.2680153877372052 No Hit CATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7519 0.2667382793376632 No Hit ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7330 0.26003346024006796 No Hit TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7252 0.2572663920410605 No Hit ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7183 0.2548186009419384 No Hit CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7168 0.25428647244212926 No Hit ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7057 0.2503487215435416 No Hit CCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7028 0.24931993977724393 No Hit GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7005 0.24850400941086992 No Hit AACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7002 0.24839758371090806 No Hit GCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6997 0.248220207544305 No Hit TTTTTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6965 0.2470850000780455 No Hit CATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6805 0.24140896274674797 No Hit TGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6794 0.24101873518022127 No Hit GTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6647 0.2358038758820917 No Hit TCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6610 0.2344912922492291 No Hit TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6443 0.22856692828468733 No Hit GCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6424 0.22789289885159575 No Hit TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6416 0.22760909698503087 No Hit CATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6365 0.22579986008567976 No Hit ATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5967 0.21168071722407716 No Hit GTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5952 0.21114858872426806 No Hit CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5934 0.21051003452449707 No Hit TTATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5903 0.20941030229155816 No Hit GTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5901 0.20933935182491695 No Hit AATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5779 0.20501137335980257 No Hit GATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5605 0.1988386827620165 No Hit ACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5521 0.19585876316308534 No Hit GGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5441 0.19302074449743656 No Hit GGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5341 0.1894732211653756 No Hit CCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5329 0.1890475183655283 No Hit GCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5314 0.18851538986571914 No Hit TATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5136 0.18220079833465064 No Hit AATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5018 0.17801472080281872 No Hit TCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4945 0.17542502877041422 No Hit TAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4939 0.17521217737049055 No Hit GATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4841 0.17173560450507083 No Hit CAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4651 0.164995310174155 No Hit TTTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4649 0.1649243597075138 No Hit CCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4518 0.16027710414251395 No Hit TCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4497 0.15953212424278113 No Hit GAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4406 0.15630387801060566 No Hit AGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4337 0.1538560869114836 No Hit TTATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4336 0.153820611678163 No Hit AATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4270 0.15147924627900278 No Hit GGTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4198 0.1489250294799189 No Hit TGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4192 0.14871217807999523 No Hit TTTTTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4188 0.1485702771467128 No Hit ACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4181 0.14832195051346853 No Hit TATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4115 0.14598058511430828 No Hit TCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4110 0.14580320894770524 No Hit GTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4109 0.14576773371438465 No Hit TTTTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4103 0.14555488231446098 No Hit GGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3935 0.13959504311659857 No Hit CTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3914 0.13885006321686577 No Hit TTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3905 0.1385307861169803 No Hit TTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3889 0.13796318238385052 No Hit TTATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3820 0.13551539128472848 No Hit GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3790 0.13445113428511019 No Hit CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 0.13246452121915606 No Hit TTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3681 0.13058433385316376 No Hit TTTATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3678 0.13047790815320193 No Hit GTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3671 0.13022958151995767 No Hit TTTTATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3628 0.12870414648717146 No Hit TCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3621 0.12845581985392718 No Hit TTTATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3592 0.1274270380876295 No Hit TTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3563 0.12639825632133184 No Hit TTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3448 0.12231860448946173 No Hit TTTTATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3432 0.12175100075633198 No Hit CTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3380 0.11990628862366029 No Hit ACTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3347 0.11873560592408015 No Hit TTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3307 0.11731659659125578 No Hit CATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3248 0.11522355782533983 No Hit GGTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3179 0.11277576672621777 No Hit TTTTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3166 0.11231458869304983 No Hit TTTATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3102 0.11004417376053081 No Hit ATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3093 0.10972489666064535 No Hit TTATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3087 0.10951204526072168 No Hit TCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3035 0.10766733312804998 No Hit CTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2903 0.10298460232972952 No Hit GGTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2891 0.10255889952988222 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0020220882992747443 0.0 0.0 0.0 0.0 2 0.004647255564999851 0.0 0.0 0.0 0.0 3 0.007307898064045568 0.0 0.0 0.0 0.0 4 0.012416331662213342 0.0 0.0 7.09504666412191E-5 0.0 5 0.02203011989209853 0.0 0.0 7.09504666412191E-5 0.0 6 0.04257027998473146 0.0 0.0 7.09504666412191E-5 0.0 7 0.06552275594316584 0.0 0.0 7.09504666412191E-5 0.0 8 0.1296265025535073 0.0 0.0 7.09504666412191E-5 0.0 9 0.36429517096933944 0.0 0.0 7.09504666412191E-5 0.0 10-11 0.8483724672457171 0.0 0.0 7.09504666412191E-5 0.0 12-13 1.3577790801130099 0.0 0.0 7.09504666412191E-5 0.0 14-15 1.8578734442336426 0.0 0.0 1.0642569996182865E-4 0.0 16-17 2.3459239666419287 0.0 0.0 1.419009332824382E-4 0.0 18-19 2.8743630421857285 0.0 0.0 1.419009332824382E-4 0.0 20-21 3.5518513105260694 0.0 0.0 1.419009332824382E-4 0.0 22-23 4.324697006032209 0.0 0.0 1.7737616660304774E-4 0.0 24-25 5.087857962841822 0.0 0.0 1.7737616660304774E-4 0.0 26-27 5.751918855370311 0.0 0.0 1.7737616660304774E-4 0.0 28-29 6.342652440625102 0.0 0.0 1.7737616660304774E-4 0.0 30-31 6.936951286828613 0.0 0.0 2.128513999236573E-4 0.0 32-33 7.6257384169815685 0.0 0.0 2.128513999236573E-4 0.0 34-35 8.326374275063607 0.0 0.0 2.128513999236573E-4 0.0 36-37 8.844383632011148 0.0 0.0 2.128513999236573E-4 0.0 38-39 9.21937458582665 0.0 0.0 2.128513999236573E-4 0.0 40-41 9.558996732021505 0.0 0.0 2.128513999236573E-4 0.0 42-43 9.853884608999074 0.0 0.0 2.128513999236573E-4 0.0 44-45 10.099515124510974 0.0 0.0 2.128513999236573E-4 0.0 46-47 10.316871879066348 0.0 0.0 2.3058901658396208E-4 0.0 48-49 10.52754155214079 0.0 0.0 2.4832663324426686E-4 0.0 50-51 10.738548239931774 0.0 0.0 2.4832663324426686E-4 0.0 52-53 10.966671727799953 0.0 0.0 2.4832663324426686E-4 0.0 54-55 11.17246355629281 0.0 0.0 2.4832663324426686E-4 0.0 56-57 11.36686783488975 0.0 0.0 2.4832663324426686E-4 0.0 58-59 11.554815621022339 0.0 0.0 2.4832663324426686E-4 0.0 60-61 11.739215883822867 0.0 0.0 2.4832663324426686E-4 0.0 62-63 11.923562933773415 0.0 0.0 2.4832663324426686E-4 0.0 64-65 12.097604428444326 0.0 0.0 2.4832663324426686E-4 0.0 66-67 12.267991974083213 0.0 0.0 2.4832663324426686E-4 0.0 68-69 12.42727577169275 0.0 0.0 2.4832663324426686E-4 0.0 70-71 12.586027440802479 0.0 0.0 2.4832663324426686E-4 0.0 72-73 12.746020743078427 0.0 0.0 2.4832663324426686E-4 0.0 74-75 12.908887539253346 0.0 0.0 2.4832663324426686E-4 0.0 76-77 13.072392889628034 0.0 0.0 2.4832663324426686E-4 0.0 78-79 13.226993956439252 0.0 0.0 2.4832663324426686E-4 0.0 80-81 13.379874474434418 0.0 0.0 2.4832663324426686E-4 0.0 82-83 13.538697094010786 0.0 0.0 2.4832663324426686E-4 0.0 84-85 13.697501975970496 0.0 0.0 2.4832663324426686E-4 0.0 86-87 13.861628142928296 0.0 0.0 2.4832663324426686E-4 0.0 88 13.988274725882873 0.0 0.0 2.4832663324426686E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTTCT 1680 0.0 57.357025 1 GTTCGCT 35 2.937284E-4 53.717846 3 CCCCCTA 2375 0.0 53.64159 6 GCTTTTC 1630 0.0 53.348984 1 GCCTTTT 2030 0.0 53.256653 1 CCCCTAC 3760 0.0 52.508137 7 GCCCTAC 1365 0.0 51.31198 7 TATCTCG 550 0.0 49.573074 9 ACTTTTC 2155 0.0 49.513157 1 CATTTCT 2590 0.0 48.81964 1 CACTTTT 2240 0.0 48.473682 1 CGCCCTA 490 0.0 47.970024 6 ACCTTTT 2275 0.0 47.934547 1 CCTTTTC 2500 0.0 47.192802 1 ATTTCTT 8500 0.0 47.05841 2 GCTTTCT 1605 0.0 46.85835 1 ACTTTCT 2475 0.0 46.340073 1 CATTTTC 1830 0.0 45.977356 1 CGCTTTT 805 0.0 45.54501 1 GCATTTT 1405 0.0 45.49929 1 >>END_MODULE