FastQCFastQC Report
Thu 26 May 2016
SRR1248474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4425725
Sequences flagged as poor quality0
Sequence length100
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2168874.9005982070734175No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA512091.1570759593061024No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA427150.9651526021160375No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380620.8600172853035378No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA184330.41649673217382466No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146680.33142592456603154No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130740.2954092267368623No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124710.2817843404188014No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA108510.24518016822102592No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103170.23311434849657403No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90370.20419253342672672No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73920.16702348202836823No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69180.1563133723853154No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60290.1362262680125855No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60100.13579695982014248No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52050.1176078495613713No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48370.1092928277287902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTGA26950.052.1368379
TACCCGA42600.048.5356256
GCTTGCT5650.048.2394147
GTTGCTG5950.046.5980038
CTACCCG45100.044.699055
ACCCGAC47100.044.6971247
GTACTAT1007.075869E-1042.2921831
CTTGCTG18400.041.1187938
TGCTTGC3250.040.484826
TAACGAC7900.040.4482156
CTGCTGA26950.040.2796339
CCCGACG57000.039.078318
TCGACAC7250.038.889365
CCTGCTG19350.038.857188
CCTACCC37800.038.537854
GTCACGC1600.038.1804432
CCCTACC31200.036.9002273
TTTGCTG7150.036.805638
ACTTGCT4550.036.147577
TGAAAAA17550.036.1471631
Produced by FastQC (version 0.11.5)