##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248474_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4425725
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.45892548678465	31.0	31.0	34.0	26.0	34.0
2	30.761263973699226	33.0	31.0	34.0	26.0	34.0
3	31.006673256924007	33.0	31.0	34.0	27.0	34.0
4	34.45440780889007	37.0	35.0	37.0	30.0	37.0
5	34.389182789260516	37.0	35.0	37.0	30.0	37.0
6	34.31535420750273	37.0	35.0	37.0	30.0	37.0
7	34.36923577492953	37.0	35.0	37.0	30.0	37.0
8	34.30713250371408	37.0	35.0	37.0	30.0	37.0
9	35.78086053697417	39.0	35.0	39.0	30.0	39.0
10-11	35.97296578526682	39.0	35.0	39.0	30.0	39.0
12-13	35.95346016754317	39.0	35.0	39.0	30.0	39.0
14-15	37.03783572183089	40.0	37.0	41.0	30.0	41.0
16-17	36.84046726807472	39.5	37.0	41.0	30.0	41.0
18-19	36.42491580475515	39.0	35.5	41.0	30.0	41.0
20-21	36.16126216608578	39.0	35.0	41.0	28.5	41.0
22-23	35.992012156200396	39.0	35.0	41.0	28.0	41.0
24-25	35.83879262719667	39.0	35.0	41.0	27.0	41.0
26-27	35.68081986567172	38.0	35.0	40.0	25.5	41.0
28-29	35.53101446655633	38.0	35.0	40.0	25.0	41.0
30-31	35.452569578995536	38.0	35.0	40.0	25.0	41.0
32-33	35.35415124075716	38.0	35.0	40.0	25.0	41.0
34-35	35.23013223822086	38.0	34.5	40.0	25.0	41.0
36-37	35.127241525399796	38.0	34.0	40.0	25.0	41.0
38-39	34.91998632992335	38.0	33.5	40.0	25.0	41.0
40-41	34.66041281371979	38.0	33.0	40.0	25.0	41.0
42-43	34.34690598715465	37.0	33.0	40.0	25.0	41.0
44-45	34.083173717300554	37.0	33.0	39.0	24.5	41.0
46-47	33.852839591253414	36.0	32.5	39.0	24.0	40.5
48-49	33.680977353992844	35.5	32.5	39.0	25.0	40.0
50-51	33.332896192149306	35.0	32.0	38.5	24.5	40.0
52-53	32.887495834015894	35.0	31.5	37.0	24.0	39.5
54-55	32.65562975557677	35.0	31.0	36.5	25.0	39.0
56-57	32.5034709567359	35.0	31.0	36.0	25.0	39.0
58-59	32.199933909133534	35.0	31.0	35.5	25.0	37.5
60-61	31.911073101017347	35.0	31.0	35.0	24.5	37.0
62-63	31.682218167644848	34.0	31.0	35.0	24.0	36.0
64-65	31.584836156787873	34.0	31.0	35.0	24.0	36.0
66-67	31.523729445458088	34.5	31.0	35.0	24.0	35.5
68-69	31.441240813652	35.0	31.0	35.0	23.0	35.0
70-71	31.333473272740626	35.0	31.0	35.0	23.0	35.0
72-73	31.208575205192368	34.0	30.5	35.0	23.0	35.0
74-75	31.06602014359229	34.0	30.0	35.0	23.0	35.0
76-77	30.874029113874	34.0	30.0	35.0	20.5	35.0
78-79	30.689714566539948	34.0	30.0	35.0	20.0	35.0
80-81	30.453069158160528	34.0	29.5	35.0	19.5	35.0
82-83	30.236920391574262	34.0	29.0	35.0	17.5	35.0
84-85	30.011500375644665	34.0	29.0	35.0	15.0	35.0
86-87	29.693065881861166	34.0	28.5	35.0	12.5	35.0
88-89	29.30383914499884	34.0	27.5	35.0	10.0	35.0
90-91	28.72180162572234	34.0	26.0	35.0	8.0	35.0
92-93	27.996629591761803	33.5	24.5	35.0	7.5	35.0
94-95	27.037558931022602	33.0	21.5	35.0	7.0	35.0
96-97	25.80531178507476	32.5	16.5	35.0	7.0	35.0
98-99	24.21514294268171	29.5	10.0	35.0	7.0	35.0
100	22.315604110061063	26.0	8.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5678896929322619
1101	2	0.5214690095738561
1101	3	0.41927184422045727
1101	4	0.32392055613301807
1101	5	0.24760542009434516
1101	6	0.24720072979287266
1101	7	0.23035652729886635
1101	8	0.2831274797512222
1101	9	0.2801784922582158
1101	10-11	0.40468988263322814
1101	12-13	0.38848098628673
1101	14-15	0.5276045528749123
1101	16-17	0.5374898992863422
1101	18-19	0.6089605387086507
1101	20-21	0.6200062683615073
1101	22-23	0.6010470416317162
1101	24-25	0.6200260390923802
1101	26-27	0.6541045350762715
1101	28-29	0.6096609181349422
1101	30-31	0.5318609952227717
1101	32-33	0.5654233212068007
1101	34-35	0.5742382220820375
1101	36-37	0.5656377247962112
1101	38-39	0.5527943424391282
1101	40-41	0.5610619220727102
1101	42-43	0.5917394833319491
1101	44-45	0.6051509050619046
1101	46-47	0.6439560696287785
1101	48-49	0.6073540417704848
1101	50-51	0.5725916372049937
1101	52-53	0.559322430537371
1101	54-55	0.5135843672084519
1101	56-57	0.4995017514237503
1101	58-59	0.47355268011541085
1101	60-61	0.427162288056671
1101	62-63	0.5333216501934359
1101	64-65	0.4732944215604782
1101	66-67	0.4287249809747564
1101	68-69	0.4355331974154524
1101	70-71	0.44388247978085005
1101	72-73	0.3965558314994162
1101	74-75	0.4772342712863349
1101	76-77	0.44598359069966875
1101	78-79	0.45154829197737456
1101	80-81	0.4685519729110368
1101	82-83	0.41544138043605017
1101	84-85	0.42119355475175624
1101	86-87	0.4562120011597308
1101	88-89	0.4803867206860666
1101	90-91	0.4867934916303298
1101	92-93	0.43042130553564917
1101	94-95	0.3143038027318461
1101	96-97	0.2977893447508464
1101	98-99	0.29325742148065714
1101	100	0.3065316902264783
1102	1	0.38166632183871485
1102	2	0.39723695111359447
1102	3	0.37086243822414744
1102	4	0.3858914001921434
1102	5	0.4402172851032802
1102	6	0.3100497701536895
1102	7	0.3711723190116629
1102	8	0.3095624543346034
1102	9	0.24895635904104552
1102	10-11	0.4185835886348457
1102	12-13	0.564358705513186
1102	14-15	0.7161002191109418
1102	16-17	0.7168203048368156
1102	18-19	0.7220416013790683
1102	20-21	0.6975820384569715
1102	22-23	0.6985347351376774
1102	24-25	0.7144397936215299
1102	26-27	0.7372861280465131
1102	28-29	0.7329992084320978
1102	30-31	0.7839696519873911
1102	32-33	0.7626721370053176
1102	34-35	0.7762986800780425
1102	36-37	0.7365528697083619
1102	38-39	0.7905321707385227
1102	40-41	0.7829683789253608
1102	42-43	0.7822424868905244
1102	44-45	0.7107410049227241
1102	46-47	0.7376139512044162
1102	48-49	0.7655649410154055
1102	50-51	0.7650826431560631
1102	52-53	0.7031103694331975
1102	54-55	0.7023055656415735
1102	56-57	0.6945370956129011
1102	58-59	0.6655746250720895
1102	60-61	0.614759273671794
1102	62-63	0.6048928538444827
1102	64-65	0.5380733807983553
1102	66-67	0.5677183453149368
1102	68-69	0.6015086928163527
1102	70-71	0.6557404254829464
1102	72-73	0.591583932962326
1102	74-75	0.6335844826700239
1102	76-77	0.6021376816528168
1102	78-79	0.6399212884739711
1102	80-81	0.6278605702978979
1102	82-83	0.5678843771889781
1102	84-85	0.5397553930539232
1102	86-87	0.5008846642578959
1102	88-89	0.4571131961065511
1102	90-91	0.46681646240895347
1102	92-93	0.32930861180138393
1102	94-95	0.3646438676228918
1102	96-97	0.4660573676925317
1102	98-99	0.4888751280946977
1102	100	0.42051525956667035
1103	1	0.4650918785106981
1103	2	0.40106740195450286
1103	3	0.41575320598851917
1103	4	0.4145920856011571
1103	5	0.42947471466493425
1103	6	0.3160427534446626
1103	7	0.3778522020599908
1103	8	0.367554167141094
1103	9	0.40757922712745653
1103	10-11	0.5487175582417905
1103	12-13	0.5474309549971252
1103	14-15	0.6954487706955064
1103	16-17	0.7141476729272114
1103	18-19	0.6558416335590707
1103	20-21	0.6891379904387662
1103	22-23	0.6357451562993717
1103	24-25	0.6781947100879435
1103	26-27	0.7463078778173227
1103	28-29	0.725357259694789
1103	30-31	0.6611805781657765
1103	32-33	0.7405838212629519
1103	34-35	0.7817470959275212
1103	36-37	0.7460510363406954
1103	38-39	0.745457297123842
1103	40-41	0.6765191438759786
1103	42-43	0.7611247723305183
1103	44-45	0.8216563331543796
1103	46-47	0.8055158312031452
1103	48-49	0.8399487724910983
1103	50-51	0.8255747516923293
1103	52-53	0.7101454386636092
1103	54-55	0.7635691286665249
1103	56-57	0.7510181298207002
1103	58-59	0.7230158726482472
1103	60-61	0.7640229436275021
1103	62-63	0.7698908463734924
1103	64-65	0.7320333100355576
1103	66-67	0.6949531219735263
1103	68-69	0.6564449805447552
1103	70-71	0.657156294903416
1103	72-73	0.6522008730893099
1103	74-75	0.614508795990325
1103	76-77	0.6225268562880935
1103	78-79	0.6590186843512527
1103	80-81	0.6521272743547684
1103	82-83	0.6253461336589652
1103	84-85	0.510370816056227
1103	86-87	0.4628889895598931
1103	88-89	0.47657548432592023
1103	90-91	0.42941461206217113
1103	92-93	0.3814471841138598
1103	94-95	0.33576121023691385
1103	96-97	0.30193845688905796
1103	98-99	0.26603206727743967
1103	100	0.30205013925281676
1104	1	0.15974628520195466
1104	2	0.16762917793966636
1104	3	0.206196796661402
1104	4	0.18074048727896752
1104	5	0.11099043434458622
1104	6	0.11038551378414496
1104	7	0.05913129283494101
1104	8	0.14238529292462232
1104	9	0.24730118460156802
1104	10-11	0.3168057468801422
1104	12-13	0.41475305413065655
1104	14-15	0.44231438323713235
1104	16-17	0.47565389730337415
1104	18-19	0.47682260370579144
1104	20-21	0.40216020198336366
1104	22-23	0.41826136348828413
1104	24-25	0.3683433012004329
1104	26-27	0.42736487890854846
1104	28-29	0.39675587410291513
1104	30-31	0.37242922400383804
1104	32-33	0.3444028073313916
1104	34-35	0.3914840519578391
1104	36-37	0.4182806885022359
1104	38-39	0.40397576503143995
1104	40-41	0.4748506524754177
1104	42-43	0.5247888445752977
1104	44-45	0.5083116194890636
1104	46-47	0.5039874891227072
1104	48-49	0.5425338925727274
1104	50-51	0.46179924954243745
1104	52-53	0.4353060223723517
1104	54-55	0.4213977852216786
1104	56-57	0.42627347471788113
1104	58-59	0.493602875127344
1104	60-61	0.42418760489571383
1104	62-63	0.4604308179670582
1104	64-65	0.35323343461516643
1104	66-67	0.3798653324854442
1104	68-69	0.4429621593537654
1104	70-71	0.4522713879796427
1104	72-73	0.43185849951229116
1104	74-75	0.4341490060435582
1104	76-77	0.4509695163412104
1104	78-79	0.4495897569965983
1104	80-81	0.44947112997309446
1104	82-83	0.4309837446859923
1104	84-85	0.3119015836240493
1104	86-87	0.3072449982677483
1104	88-89	0.2831729031907635
1104	90-91	0.24631002000338853
1104	92-93	0.19224761632748866
1104	94-95	0.2124362216764446
1104	96-97	0.2827912072766452
1104	98-99	0.3385263497048072
1104	100	0.4554004416795756
1105	1	0.29916267015884657
1105	2	0.263483917798478
1105	3	0.2782803264822533
1105	4	0.23622553341392205
1105	5	0.26655736839995825
1105	6	0.16807789913950444
1105	7	0.2996699399363152
1105	8	0.21227095841723553
1105	9	0.2848476193394376
1105	10-11	0.2627693755132441
1105	12-13	0.3234796187874238
1105	14-15	0.4085721694757041
1105	16-17	0.4517209021874464
1105	18-19	0.48715600911042856
1105	20-21	0.4628759192865459
1105	22-23	0.4466805647144909
1105	24-25	0.4704814737080909
1105	26-27	0.4910374615321942
1105	28-29	0.5582323449945008
1105	30-31	0.48615838674852085
1105	32-33	0.4656842616434602
1105	34-35	0.5242046522994386
1105	36-37	0.5203776348739311
1105	38-39	0.5038846585069408
1105	40-41	0.5140543261033841
1105	42-43	0.4943919926004483
1105	44-45	0.46889319458270506
1105	46-47	0.5341430937751142
1105	48-49	0.47443222501242843
1105	50-51	0.4404595919515799
1105	52-53	0.39879725659562126
1105	54-55	0.3264593916953302
1105	56-57	0.37432865990697906
1105	58-59	0.35519829733578234
1105	60-61	0.35139014453960016
1105	62-63	0.33065380256430643
1105	64-65	0.24224933710848262
1105	66-67	0.2055215050100756
1105	68-69	0.2798369536761207
1105	70-71	0.3532928978487533
1105	72-73	0.3819222194564311
1105	74-75	0.39393259329364483
1105	76-77	0.3516264240760556
1105	78-79	0.3708361749178337
1105	80-81	0.39410370452807086
1105	82-83	0.2619264022244536
1105	84-85	0.28347862868373497
1105	86-87	0.3283083048373605
1105	88-89	0.3491758044752302
1105	90-91	0.4391162832074009
1105	92-93	0.40789273170475226
1105	94-95	0.4623236620286342
1105	96-97	0.39403869901899213
1105	98-99	0.4292809052640223
1105	100	0.4937399197134624
1106	1	0.17727433909721313
1106	2	0.13223663609132075
1106	3	0.05873183506213664
1106	4	0.1426637213237072
1106	5	0.21019230762065888
1106	6	0.0611627833019881
1106	7	0.023895129870638243
1106	8	0.1347459775225346
1106	9	-0.026386667395136953
1106	10-11	0.18050563566177402
1106	12-13	0.2007315214480414
1106	14-15	0.2884948459240064
1106	16-17	0.29665998060498566
1106	18-19	0.3488703409246554
1106	20-21	0.2724661141936906
1106	22-23	0.22659466254449967
1106	24-25	0.2541726455136768
1106	26-27	0.36675251123743635
1106	28-29	0.332188750918597
1106	30-31	0.31400244874861016
1106	32-33	0.23080684090838588
1106	34-35	0.3511724223248365
1106	36-37	0.34267440744340405
1106	38-39	0.34977237336280353
1106	40-41	0.3400497093765651
1106	42-43	0.38071957457349015
1106	44-45	0.40446387629092584
1106	46-47	0.39829535292143703
1106	48-49	0.4097996713651568
1106	50-51	0.37300696805302636
1106	52-53	0.3096820242255305
1106	54-55	0.29355482463406446
1106	56-57	0.27519390362390084
1106	58-59	0.3349666299180285
1106	60-61	0.32360305134726985
1106	62-63	0.33805287927300043
1106	64-65	0.2990326638917402
1106	66-67	0.20508015217745168
1106	68-69	0.2344816685161888
1106	70-71	0.19758036004944657
1106	72-73	0.23832316495728634
1106	74-75	0.2948676135406352
1106	76-77	0.2908770037471271
1106	78-79	0.28465343668529286
1106	80-81	0.3277992663942726
1106	82-83	0.33140297553162057
1106	84-85	0.3215009522095045
1106	86-87	0.4045164764246678
1106	88-89	0.27044180604955415
1106	90-91	0.262058590045779
1106	92-93	0.2409863748552148
1106	94-95	0.21175066266820508
1106	96-97	0.4084201714249218
1106	98-99	0.3393603369786007
1106	100	0.13295864472141616
1107	1	-0.08222820803246123
1107	2	-0.11893671693960428
1107	3	0.010177345862079079
1107	4	-0.005805299550466714
1107	5	-0.024096930088802537
1107	6	0.0277449815205415
1107	7	0.00892290348028979
1107	8	-0.005770241921673858
1107	9	-0.013788278184080127
1107	10-11	0.00972141808157545
1107	12-13	0.07125629904421515
1107	14-15	0.06717687637296876
1107	16-17	0.13618774540675815
1107	18-19	0.040419366901026876
1107	20-21	0.016404420037105183
1107	22-23	0.0017432216251265231
1107	24-25	-0.023622983855496216
1107	26-27	0.050520908133634634
1107	28-29	0.056309821213233135
1107	30-31	-0.06193325939348426
1107	32-33	-0.17272343037944182
1107	34-35	-0.014416001146891233
1107	36-37	-0.09216072815782184
1107	38-39	-0.046782182244236026
1107	40-41	-0.02538099752547396
1107	42-43	-0.11512924654820722
1107	44-45	-0.024862911427447898
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2216	86-87	-0.4780413664976564
2216	88-89	-0.47160144790925784
2216	90-91	-0.24072501415343694
2216	92-93	-0.30409794208977914
2216	94-95	-0.24003603046906719
2216	96-97	-0.22680033208506245
2216	98-99	-0.21783349108706318
2216	100	-0.36729604806408034
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	2.0
4	12.0
5	117.0
6	969.0
7	2588.0
8	5501.0
9	9804.0
10	15131.0
11	18808.0
12	20232.0
13	22197.0
14	23488.0
15	24086.0
16	24244.0
17	23903.0
18	25312.0
19	26743.0
20	29214.0
21	31857.0
22	34791.0
23	40499.0
24	60159.0
25	71211.0
26	65886.0
27	105362.0
28	90591.0
29	136319.0
30	152628.0
31	200815.0
32	256156.0
33	371806.0
34	432929.0
35	834537.0
36	749628.0
37	489573.0
38	26273.0
39	2324.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.78345670309012	27.063732002928337	25.199854486953537	29.95295680702801
2	15.863068923996448	28.077111399979348	26.6167777714762	29.443041904548
3	14.181195376119593	29.468448071726183	22.763075838507632	33.58728071364659
4	12.9436983725505	27.340749413990746	22.923638259998764	36.791913953459996
5	12.739094999456583	27.85528342528491	23.7739846293207	35.63163694593781
6	12.614925686097026	25.22149108635362	24.445136866917355	37.718446360632
7	17.668899239575513	27.759380127736126	20.59466035321982	33.97706027946854
8	14.419592229249654	28.575134222523896	18.648169442252062	38.35710410597439
9	11.25364461789582	26.857760934757497	20.01604716447372	41.872547282872965
10-11	19.217375025942175	37.25308614428627	18.41079230590989	25.118746523861656
12-13	20.9742838475837	38.961513610954604	14.84787145211421	25.21633108934748
14-15	24.213712201221707	36.062699661922004	14.329642087433903	25.393946049422382
16-17	23.172382003608497	34.86975403696393	16.93821154560953	25.01965241381804
18-19	23.206953704111687	31.828267866968098	20.39153281339855	24.573245615521667
20-21	21.18837580324862	32.036882693191764	22.148438457685828	24.62630304587379
22-23	22.637100647410545	34.641034166686715	21.728931759609278	20.992933426293455
24-25	24.081834683355467	35.83781702471798	19.147864764139868	20.932483527786687
26-27	27.44459500240713	36.91255042916552	17.091316574209667	18.551537994217686
28-29	27.001696946524433	38.81131037060413	17.96278739426003	16.224205288611408
30-31	27.88658058831817	38.948846402720534	17.42350450290539	15.741068506055905
32-33	28.963988347539615	39.01513366851726	16.421199180219606	15.599678803723519
34-35	29.759883070193816	38.581740248335734	15.892942247604017	15.765434433866437
36-37	31.316006916224893	38.477788751428875	14.722874759632035	15.483329572714197
38-39	32.30801892425767	39.267529586241736	14.087438313859623	14.337013175640978
40-41	32.94129011920943	39.02735455850934	14.077649390281794	13.953705931999444
42-43	33.3814707265372	37.67718511264308	15.116493470721116	13.824850690098604
44-45	33.454984604534886	35.89835162941789	16.40687819646699	14.239785569580238
46-47	33.3669159280812	34.77877461002179	17.95622211061919	13.898087351277821
48-49	33.29543894614286	35.32874284749091	18.987912549154522	12.387905657211713
50-51	34.155154504413815	36.22076823671342	18.723456611028222	10.900620647844544
52-53	34.84904769748846	37.59791184663014	17.702122928810674	9.850917527070727
54-55	34.27619805130245	39.571022162367356	16.799562537283755	9.35321724904644
56-57	33.26785938840444	40.679442164248876	16.21381180494662	9.83888664240007
58-59	32.4323435714134	39.6605913986027	17.262439287617273	10.644625742366634
60-61	32.176924427523865	37.496827956734116	19.247721269648874	11.078526346093138
62-63	31.776565964435598	35.52891268526327	20.73992774314378	11.954593607157351
64-65	30.502210658820506	34.75858900637465	21.385264089864005	13.35393624494084
66-67	28.80349742058114	33.45277859661401	22.540806949827452	15.2029170329774
68-69	28.535212470696386	32.010303663861386	22.14669682108604	17.307787044356193
70-71	29.042374212326404	30.82925914334106	22.223417393279195	17.90494925105334
72-73	30.140524462858437	31.092992149169618	21.983477573688678	16.783005814283264
74-75	29.720327565690376	32.17844879184361	21.297984641389288	16.803239001076737
76-77	27.453617663486657	33.98984085329329	20.878500759173217	17.678040724046838
78-79	25.245900564949537	36.24551784517126	19.99219870874166	18.51638288113754
80-81	22.790542888379754	39.87955149628365	19.498102984092185	17.831802631244404
82-83	20.166864680143636	43.67271796153619	20.107494537020234	16.052922821299937
84-85	17.6825584116519	47.50632426355537	20.59165749775104	14.219459827041689
86-87	15.793942492162962	51.3506754959464	20.203000210403896	12.652381801486747
88-89	14.645950669330746	55.298609449074796	18.785217749169284	11.270222132425179
90-91	14.262118395354188	59.226096444541476	16.35716129218889	10.154623867915445
92-93	14.301495468890737	61.92843314143387	14.07685252179223	9.693218867883166
94-95	14.75258327696675	62.91893856204306	12.551190387676295	9.7772877733139
96-97	15.42466860822971	62.364356419085176	11.999542124697344	10.211432847987773
98-99	16.57362541551608	60.413015469887696	11.84918865181487	11.164170462781353
100	18.429621942507744	58.37903107159944	11.301814342396646	11.889532643496171
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	298504.0
1	254347.0
2	129278.0
3	31735.0
4	14268.5
5	12165.5
6	9107.5
7	5773.5
8	3425.5
9	2336.5
10	1981.5
11	1854.5
12	1838.5
13	1840.0
14	1810.5
15	1795.5
16	1805.5
17	1868.5
18	1893.0
19	1870.5
20	1886.0
21	1892.5
22	1914.0
23	2062.5
24	2186.0
25	2191.0
26	2262.0
27	2392.0
28	2510.5
29	2631.5
30	2782.0
31	3115.5
32	3649.0
33	4354.0
34	5288.5
35	6654.0
36	8759.0
37	11932.0
38	16608.5
39	23262.5
40	32916.0
41	47523.0
42	69227.5
43	100576.0
44	143247.5
45	196736.5
46	254382.0
47	303886.5
48	332110.0
49	330954.0
50	306083.0
51	272198.0
52	240177.0
53	215470.0
54	194761.5
55	170714.5
56	141248.0
57	107742.0
58	75077.5
59	48038.0
60	28649.5
61	16560.5
62	9872.0
63	6413.0
64	4536.5
65	3501.5
66	2977.5
67	2591.5
68	2244.0
69	2029.5
70	1881.5
71	1678.0
72	1542.0
73	1438.5
74	1313.5
75	1226.0
76	1135.5
77	1043.0
78	1018.5
79	967.5
80	919.0
81	891.0
82	827.5
83	747.0
84	694.5
85	694.5
86	666.0
87	649.0
88	645.5
89	601.5
90	529.5
91	477.5
92	465.0
93	438.0
94	399.0
95	375.5
96	345.5
97	319.0
98	280.5
99	212.5
100	184.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1297584011659106E-4
2	5.422840325596371E-4
3	1.1297584011659106E-4
4	6.100695366295918E-4
5	5.874743686062736E-4
6	8.812115529094103E-4
7	8.812115529094103E-4
8	0.0011749487372125472
9	4.744985284896825E-4
10-11	3.728202723847505E-4
12-13	9.828898090143423E-4
14-15	0.0026888249947748673
16-17	0.0013557100813990927
18-19	0.0038411785639640966
20-21	0.004078427828208938
22-23	0.0038750713159990736
24-25	0.005468030661643008
26-27	0.007558083703799942
28-29	0.009569053657875264
30-31	0.009388292313688717
32-33	0.008891198617175718
34-35	0.011433155019799015
36-37	0.010811787899157765
38-39	0.009026769625315626
40-41	0.007230453767461828
42-43	0.004473843268617006
44-45	0.005275971733444803
46-47	0.007173965847403534
48-49	0.006044207446237623
50-51	0.0042478915883838245
52-53	0.0042478915883838245
54-55	0.004632009444780234
56-57	0.006789847991007123
58-59	0.007930903976184693
60-61	0.007772737800021465
62-63	0.009060662377350604
64-65	0.010405074874738037
66-67	0.009184935801478854
68-69	0.01136536951572906
70-71	0.011523535691892288
72-73	0.01328595879771111
74-75	0.017635528642199867
76-77	0.019104214563715548
78-79	0.018448954691039323
80-81	0.022877607623609693
82-83	0.021284648277965758
84-85	0.02036954397302137
86-87	0.020380841557033028
88-89	0.021058696597732574
90-91	0.02085534008552271
92-93	0.020911828005581005
94-95	0.01857322811516757
96-97	0.02122816035790746
98-99	0.01821170542679448
100	0.01843765710702766
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4425725.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.39002400839483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.73013129427622	84.30568281570221
2	1.1212237888131453	1.9148265249107579
3	0.06075018397938976	0.15562379005543484
4	0.01936485410379676	0.06614261427369075
5	0.01354342690408444	0.05782367742478555
6	0.006169745726797081	0.03161008414421365
7	0.0067743325810373884	0.04049222952149437
8	0.0033508021647657062	0.022890006183738
9	0.0036449317657753825	0.02801167298896688
>10	0.02850855488573622	0.45941516975294544
>50	0.002325594410289509	0.15080177025974786
>100	0.0025412799870623286	0.43701778237124833
>500	5.570702543608535E-4	0.31417689604891613
>1k	6.89705805605111E-4	1.3856171486370201
>5k	1.591628782165426E-4	0.9194485243059933
>10k+	2.652714636942377E-4	9.71041929341882
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	216887	4.9005982070734175	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51209	1.1570759593061024	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42715	0.9651526021160375	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38062	0.8600172853035378	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18433	0.41649673217382466	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14668	0.33142592456603154	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13074	0.2954092267368623	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12471	0.2817843404188014	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10851	0.24518016822102592	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10317	0.23311434849657403	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9037	0.20419253342672672	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7392	0.16702348202836823	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6918	0.1563133723853154	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6029	0.1362262680125855	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6010	0.13579695982014248	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5205	0.1176078495613713	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4837	0.1092928277287902	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003457060707567687	0.0	0.0	0.0	0.0
2	0.008834710697117422	0.0	0.0	0.0	0.0
3	0.014460907534923656	0.0	0.0	0.0	0.0
4	0.024764304153556762	0.0	0.0	2.2595168023318213E-5	0.0
5	0.04082946861813601	0.0	0.0	2.2595168023318213E-5	0.0
6	0.07081325658507928	0.0	0.0	2.2595168023318213E-5	0.0
7	0.10870535336018393	0.0	0.0	2.2595168023318213E-5	0.0
8	0.2362098865157686	0.0	0.0	2.2595168023318213E-5	0.0
9	0.6982358822565794	0.0	0.0	2.2595168023318213E-5	0.0
10-11	1.7246552824678443	0.0	0.0	2.2595168023318213E-5	0.0
12-13	3.1569968762180207	0.0	0.0	2.2595168023318213E-5	0.0
14-15	4.897626942478351	0.0	0.0	2.2595168023318213E-5	0.0
16-17	6.820114218574358	0.0	0.0	2.2595168023318213E-5	0.0
18-19	8.904789610741744	0.0	0.0	2.2595168023318213E-5	0.0
20-21	11.661377514418543	0.0	0.0	2.2595168023318213E-5	0.0
22-23	14.92301261375255	0.0	0.0	2.2595168023318213E-5	0.0
24-25	18.473594721768748	0.0	0.0	2.2595168023318213E-5	0.0
26-27	22.60133650418858	0.0	0.0	2.2595168023318213E-5	0.0
28-29	27.996011952843883	0.0	0.0	2.2595168023318213E-5	0.0
30-31	34.81088409243684	0.0	0.0	2.2595168023318213E-5	0.0
32-33	42.00223692163431	0.0	0.0	2.2595168023318213E-5	0.0
34-35	47.846488428449575	0.0	0.0	2.2595168023318213E-5	0.0
36-37	51.89164487174418	0.0	0.0	2.2595168023318213E-5	0.0
38-39	54.58512447113185	0.0	0.0	2.2595168023318213E-5	0.0
40-41	56.61374125143338	0.0	0.0	2.2595168023318213E-5	0.0
42-43	58.11620694914393	0.0	0.0	2.2595168023318213E-5	0.0
44-45	59.268447542493035	0.0	0.0	2.2595168023318213E-5	0.0
46-47	60.20961537375232	0.0	0.0	2.2595168023318213E-5	0.0
48-49	61.03719729535838	0.0	0.0	3.389275203497732E-5	0.0
50-51	61.87031503313017	0.0	0.0	4.5190336046636426E-5	0.0
52-53	62.77685576939372	0.0	0.0	4.5190336046636426E-5	0.0
54-55	63.73095933434635	0.0	0.0	7.908308808161375E-5	0.0
56-57	64.75019347112621	0.0	0.0	9.038067209327285E-5	0.0
58-59	65.79325421258663	0.0	0.0	9.038067209327285E-5	0.0
60-61	66.81736890565952	0.0	0.0	9.038067209327285E-5	0.0
62-63	67.75001835857401	0.0	0.0	9.038067209327285E-5	0.0
64-65	68.5701438747324	0.0	0.0	9.038067209327285E-5	2.2595168023318213E-5
66-67	69.27651401747735	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
68-69	69.85585412559524	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
70-71	70.35356918922889	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
72-73	70.80435408887809	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
74-75	71.21592507442284	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
76-77	71.60447158375182	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
78-79	71.96859271644759	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
80-81	72.30967807534358	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
82-83	72.63496941179129	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
84-85	72.95597896389856	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
86-87	73.2676341164442	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
88	73.50201379435008	2.2595168023318213E-5	0.0	9.038067209327285E-5	2.2595168023318213E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTGA	2695	0.0	52.136837	9
TACCCGA	4260	0.0	48.535625	6
GCTTGCT	565	0.0	48.239414	7
GTTGCTG	595	0.0	46.598003	8
CTACCCG	4510	0.0	44.69905	5
ACCCGAC	4710	0.0	44.697124	7
GTACTAT	100	7.075869E-10	42.292183	1
CTTGCTG	1840	0.0	41.118793	8
TGCTTGC	325	0.0	40.48482	6
TAACGAC	790	0.0	40.448215	6
CTGCTGA	2695	0.0	40.279633	9
CCCGACG	5700	0.0	39.07831	8
TCGACAC	725	0.0	38.88936	5
CCTGCTG	1935	0.0	38.85718	8
CCTACCC	3780	0.0	38.53785	4
GTCACGC	160	0.0	38.180443	2
CCCTACC	3120	0.0	36.900227	3
TTTGCTG	715	0.0	36.80563	8
ACTTGCT	455	0.0	36.14757	7
TGAAAAA	1755	0.0	36.147163	1
>>END_MODULE