##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248473_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2086192
Sequences flagged as poor quality	0
Sequence length	100
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.05262842537983	31.0	30.0	33.0	16.0	34.0
2	29.70931199045917	31.0	30.0	34.0	23.0	34.0
3	30.18064444691572	31.0	30.0	34.0	25.0	34.0
4	33.76939754346676	35.0	35.0	37.0	28.0	37.0
5	33.556649148304665	35.0	35.0	37.0	28.0	37.0
6	33.2841253345809	35.0	33.0	37.0	27.0	37.0
7	33.45136833043171	35.0	33.0	37.0	28.0	37.0
8	33.38063994109842	35.0	33.0	37.0	27.0	37.0
9	34.692142429843464	37.0	34.0	39.0	27.0	39.0
10-11	35.40673701174197	38.5	35.0	39.0	30.0	39.0
12-13	35.61441372606165	39.0	35.0	39.0	30.0	39.0
14-15	36.56912355142767	39.0	36.0	41.0	30.0	41.0
16-17	36.25819603373036	39.0	35.5	40.5	30.0	41.0
18-19	35.60349023483936	37.5	34.0	40.0	29.0	41.0
20-21	35.01519251344076	37.0	33.5	39.5	27.0	41.0
22-23	34.496341180485786	36.5	33.0	39.0	27.0	40.5
24-25	34.03904913833434	35.0	33.0	39.0	25.0	40.0
26-27	33.74711963232531	35.0	32.5	39.0	25.0	40.0
28-29	33.525639778122056	35.0	32.0	38.0	25.0	40.0
30-31	33.39265153926388	35.0	32.0	38.0	25.0	40.0
32-33	33.25456357804076	35.0	31.5	38.0	25.0	40.0
34-35	33.06540097939212	35.0	31.0	38.0	24.0	40.0
36-37	32.84015876774525	35.0	30.5	38.0	23.5	40.0
38-39	32.57317758863997	35.0	30.0	38.0	23.0	40.0
40-41	32.29311396074762	35.0	30.0	38.0	22.5	40.0
42-43	31.992608781933782	35.0	28.5	37.0	22.0	39.0
44-45	31.632360060818947	34.5	28.0	37.0	22.0	39.0
46-47	31.465105560753756	34.0	28.0	36.5	21.5	39.0
48-49	31.46603596409151	34.0	28.0	36.0	22.0	39.0
50-51	31.324421242148375	34.0	28.0	36.0	22.0	39.0
52-53	31.19657442843228	34.0	28.0	35.0	22.0	39.0
54-55	31.003529876444738	34.0	28.0	35.0	21.0	38.0
56-57	31.24370791374907	34.0	29.0	35.0	22.5	37.0
58-59	31.328510511017203	34.0	30.0	35.0	23.0	37.0
60-61	31.14904141133702	34.0	29.5	35.0	23.0	36.0
62-63	30.94950488737374	34.0	29.0	35.0	23.0	35.5
64-65	30.80047929433149	33.5	29.0	35.0	23.0	35.0
66-67	30.68261837836594	33.0	29.0	35.0	23.0	35.0
68-69	30.595453582412357	33.0	29.0	35.0	23.0	35.0
70-71	30.54675816032273	33.0	29.0	35.0	23.0	35.0
72-73	30.50940876966262	33.0	29.0	35.0	23.0	35.0
74-75	30.466042674883234	33.0	29.0	35.0	22.0	35.0
76-77	30.344699577028386	33.0	29.0	35.0	20.0	35.0
78-79	30.193512390038883	33.0	29.0	35.0	20.0	35.0
80-81	30.04891879558545	33.0	29.0	35.0	19.5	35.0
82-83	29.876427481267307	33.0	28.0	35.0	18.5	35.0
84-85	29.688704347442613	33.0	28.0	35.0	17.0	35.0
86-87	29.39257077009211	33.0	28.0	35.0	15.0	35.0
88-89	29.059097868269077	33.0	27.0	35.0	10.0	35.0
90-91	28.616602882189177	33.0	26.5	35.0	10.0	35.0
92-93	28.16967949258745	33.0	25.0	35.0	8.0	35.0
94-95	27.782834465859324	33.0	25.0	35.0	7.5	35.0
96-97	27.33744545085016	33.0	24.0	35.0	7.0	35.0
98-99	26.837871346453248	33.0	22.0	35.0	7.0	35.0
100	25.37043282689225	30.0	17.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6267382590216286
1101	2	0.45639646710106163
1101	3	0.7293519056280324
1101	4	0.6279107911501214
1101	5	0.5348027160030071
1101	6	0.48638067866320256
1101	7	0.44728533567801065
1101	8	0.5362609626740849
1101	9	0.757547896970415
1101	10-11	0.8492457691322812
1101	12-13	0.8425810988336551
1101	14-15	1.015011136038538
1101	16-17	1.0245893525884
1101	18-19	1.0433472860251243
1101	20-21	0.8569557384362483
1101	22-23	0.9659757551223507
1101	24-25	1.0018077859603807
1101	26-27	1.0178592473115486
1101	28-29	1.03356039114513
1101	30-31	1.0639704547123614
1101	32-33	1.0547404728324636
1101	34-35	1.1189539280945837
1101	36-37	1.0746874628580514
1101	38-39	0.9772797752768057
1101	40-41	1.129140405852425
1101	42-43	1.0388413853199552
1101	44-45	0.9753318258338197
1101	46-47	1.1528813603299355
1101	48-49	1.1203075638901083
1101	50-51	1.0115884018683374
1101	52-53	1.007886742455561
1101	54-55	0.9974920437156314
1101	56-57	0.9110264680806708
1101	58-59	0.838749053793002
1101	60-61	0.8446175708267205
1101	62-63	0.8559417927242485
1101	64-65	0.8143367639131114
1101	66-67	0.8427420135841714
1101	68-69	0.8263794901267651
1101	70-71	0.8182733048312087
1101	72-73	0.8803388935346241
1101	74-75	0.7022094806692571
1101	76-77	0.6902286519714025
1101	78-79	0.7731792402989228
1101	80-81	0.7953207252941681
1101	82-83	0.8200282977973608
1101	84-85	0.8225642074783295
1101	86-87	0.7380623027956297
1101	88-89	0.6815133240807185
1101	90-91	0.6423289073090679
1101	92-93	0.6034367794522382
1101	94-95	0.542672452441483
1101	96-97	0.5230734354018729
1101	98-99	0.6634090376081865
1101	100	0.7721881237256092
1102	1	0.4157987656808757
1102	2	0.2880566712505903
1102	3	0.26434092170652335
1102	4	0.33643764635358764
1102	5	0.17368448835160422
1102	6	0.1773400703745196
1102	7	0.2851599822304536
1102	8	0.14497899521876434
1102	9	0.2808415121501824
1102	10-11	0.28772402054855917
1102	12-13	0.5060666719910714
1102	14-15	0.5709459638977279
1102	16-17	0.6731799632609068
1102	18-19	0.6345416021535399
1102	20-21	0.5038996777373086
1102	22-23	0.4174049747111894
1102	24-25	0.4165520698940952
1102	26-27	0.36317325439176074
1102	28-29	0.4593252707830402
1102	30-31	0.5077901717028368
1102	32-33	0.5054071311164563
1102	34-35	0.528879222758647
1102	36-37	0.47400003126196566
1102	38-39	0.5227074461667698
1102	40-41	0.5662246449074004
1102	42-43	0.606137354983062
1102	44-45	0.5629800092299391
1102	46-47	0.5423355120846125
1102	48-49	0.6168246941448032
1102	50-51	0.5957632770058012
1102	52-53	0.584750354790458
1102	54-55	0.4770691011772854
1102	56-57	0.5040926089441591
1102	58-59	0.5348906402872586
1102	60-61	0.5668648974088626
1102	62-63	0.575509414439356
1102	64-65	0.5272309918662863
1102	66-67	0.6290072916610185
1102	68-69	0.6268709505403756
1102	70-71	0.6537812869698243
1102	72-73	0.629792317044565
1102	74-75	0.4592349467065979
1102	76-77	0.5016746945375701
1102	78-79	0.6539495974898664
1102	80-81	0.5961252261609893
1102	82-83	0.6381621418450578
1102	84-85	0.48851873985876537
1102	86-87	0.5223366959817746
1102	88-89	0.5534641921851176
1102	90-91	0.5918010346586371
1102	92-93	0.6326979411126139
1102	94-95	0.7164074153664899
1102	96-97	0.7315160709352888
1102	98-99	0.8562727369133185
1102	100	0.8225981150338235
1103	1	0.2682172941173775
1103	2	0.3712229206101263
1103	3	0.2639994226877569
1103	4	0.25794531739311566
1103	5	0.19949340357864287
1103	6	0.08017332798030452
1103	7	0.30400574938274616
1103	8	0.1979345447590859
1103	9	0.20544708440876036
1103	10-11	0.3620400557763688
1103	12-13	0.47638465518922146
1103	14-15	0.4903447032757242
1103	16-17	0.5115729844033865
1103	18-19	0.44441993859653905
1103	20-21	0.421222475274071
1103	22-23	0.350034444650575
1103	24-25	0.4380237300675276
1103	26-27	0.369180422729265
1103	28-29	0.43824139205023727
1103	30-31	0.32329635397098855
1103	32-33	0.35785393654176545
1103	34-35	0.46446907591165143
1103	36-37	0.479905934143801
1103	38-39	0.3606376430906195
1103	40-41	0.2412599307225065
1103	42-43	0.3422978232693339
1103	44-45	0.07078989602531038
1103	46-47	0.1586840827674152
1103	48-49	0.3839530571730023
1103	50-51	0.457211348853523
1103	52-53	0.4790655924621774
1103	54-55	0.36831991081756144
1103	56-57	0.33113012308265155
1103	58-59	0.2103404135879785
1103	60-61	0.2249229933282777
1103	62-63	0.3551921562921976
1103	64-65	0.3321189231507873
1103	66-67	0.36127266515895684
1103	68-69	0.4091607340884842
1103	70-71	0.4354026943421445
1103	72-73	0.4290838213803667
1103	74-75	0.48446901733623093
1103	76-77	0.4363715265480863
1103	78-79	0.33206212057065443
1103	80-81	0.19302490810528639
1103	82-83	0.21686449391048868
1103	84-85	0.11872424347918198
1103	86-87	0.2664385269772218
1103	88-89	0.28610622967508803
1103	90-91	0.22463738581533477
1103	92-93	0.23764496405322788
1103	94-95	0.14547446808253284
1103	96-97	0.15854597203833265
1103	98-99	0.2174968888453357
1103	100	0.20481237243659578
1104	1	0.24214880096669233
1104	2	0.32733707586126926
1104	3	0.3459628930073926
1104	4	0.264899655292659
1104	5	0.31178564102156514
1104	6	0.20451579373373363
1104	7	0.21902401422292428
1104	8	0.19850075480474771
1104	9	0.34274388806173306
1104	10-11	0.21851265851609725
1104	12-13	0.2912819154632018
1104	14-15	0.30557757998805357
1104	16-17	0.4267259524399236
1104	18-19	0.3869998472723353
1104	20-21	0.42359233828776865
1104	22-23	0.41616914784692227
1104	24-25	0.44601231454241486
1104	26-27	0.37752517158771326
1104	28-29	0.23868659752968568
1104	30-31	0.4185543448385687
1104	32-33	0.4222831602860566
1104	34-35	0.4082750119847134
1104	36-37	0.32303378802508576
1104	38-39	0.2818180083874253
1104	40-41	0.27475993072250304
1104	42-43	0.24381837121453032
1104	44-45	0.2958195763906062
1104	46-47	0.2177365941829379
1104	48-49	0.3815900434743682
1104	50-51	0.38064742191288303
1104	52-53	0.26715919976811264
1104	54-55	0.3612400021417628
1104	56-57	0.2948082052744354
1104	58-59	0.2922627880172044
1104	60-61	0.24889559606800304
1104	62-63	0.23409398277621918
1104	64-65	0.29249563547955404
1104	66-67	0.27752152360644544
1104	68-69	0.33508995783277484
1104	70-71	0.3369848861229663
1104	72-73	0.3319650999191772
1104	74-75	0.24766308126317327
1104	76-77	0.18719116125128465
1104	78-79	0.22813061372134058
1104	80-81	0.3707965976030039
1104	82-83	0.41482339802007573
1104	84-85	0.3686009558079526
1104	86-87	0.405778709625622
1104	88-89	0.36331399223216465
1104	90-91	0.4273337328473019
1104	92-93	0.4768139138249161
1104	94-95	0.3507073447948592
1104	96-97	0.4140003099378795
1104	98-99	0.43109277925629286
1104	100	0.5967256144457274
1105	1	0.009150752820954011
1105	2	0.12442208995799575
1105	3	0.0930947516063938
1105	4	0.07741060736379524
1105	5	0.014225892593891842
1105	6	0.02599399802781477
1105	7	0.0758845650795763
1105	8	0.08278160494570841
1105	9	-0.06412381959387403
1105	10-11	0.062476549212256316
1105	12-13	0.19489284584706468
1105	14-15	0.24360425532962893
1105	16-17	0.17906514133603935
1105	18-19	0.19930737275488042
1105	20-21	0.16345928688893707
1105	22-23	0.18710029076602552
1105	24-25	0.29213777539688834
1105	26-27	0.23761842684688617
1105	28-29	0.2302903711145987
1105	30-31	0.19729680851239095
1105	32-33	0.15796088746020587
1105	34-35	0.2698665322623057
1105	36-37	0.3523566030326748
1105	38-39	0.24025023567001114
1105	40-41	0.27149740632432895
1105	42-43	0.27785610706359165
1105	44-45	0.24210866769401207
1105	46-47	0.23439675466873666
1105	48-49	0.20818568433318774
1105	50-51	0.1675499376361529
1105	52-53	0.3511713446390736
1105	54-55	0.31317222942434597
1105	56-57	0.29706324756460845
1105	58-59	0.18271410009701228
1105	60-61	0.25593040413566825
1105	62-63	0.3911373573153618
1105	64-65	0.3203551019727229
1105	66-67	0.2088652668291715
1105	68-69	0.2599589667245574
1105	70-71	0.340453005836693
1105	72-73	0.2521935753041298
1105	74-75	0.2630094269582486
1105	76-77	0.2546688233636232
1105	78-79	0.3255056950486015
1105	80-81	0.32162982249998606
1105	82-83	0.40069262378455406
1105	84-85	0.3673865771482241
1105	86-87	0.3291506462987961
1105	88-89	0.31784413753687346
1105	90-91	0.4192726831834541
1105	92-93	0.3621084269457171
1105	94-95	0.2860469674363699
1105	96-97	0.18209389050608493
1105	98-99	0.19721509545668425
1105	100	0.0320810687940245
1106	1	0.14310207199472913
1106	2	0.033870645525034604
1106	3	0.16919578590673723
1106	4	0.16723846719511926
1106	5	0.11009781203885893
1106	6	0.1587626516132019
1106	7	0.1954979641638417
1106	8	0.28027763739148526
1106	9	0.30324737930676093
1106	10-11	0.2521180404045964
1106	12-13	0.301159453329781
1106	14-15	0.2644377153409394
1106	16-17	0.31867288551543993
1106	18-19	0.24747895351360683
1106	20-21	0.3003839915265658
1106	22-23	0.23526427084617296
1106	24-25	0.120908168017543
1106	26-27	0.055998691836940395
1106	28-29	0.30928118341432054
1106	30-31	0.29213351553359246
1106	32-33	0.22569813504166802
1106	34-35	0.24744909083313615
1106	36-37	0.2540083825035424
1106	38-39	0.22695056827570426
1106	40-41	0.4303591895043297
1106	42-43	0.3090629732126047
1106	44-45	0.3117172023439849
1106	46-47	0.37871843970877705
1106	48-49	0.3809688704160408
1106	50-51	0.43129652493146864
1106	52-53	0.3224787829671669
1106	54-55	0.39204143086665155
1106	56-57	0.32302412535177893
1106	58-59	0.31006803025589846
1106	60-61	0.3610249869800235
1106	62-63	0.27878868682605784
1106	64-65	0.34166740473511226
1106	66-67	0.26203049342060325
1106	68-69	0.2852509847959297
1106	70-71	0.36595024222995676
1106	72-73	0.38701897251559103
1106	74-75	0.4032252254247375
1106	76-77	0.46558309378968943
1106	78-79	0.36936313064045123
1106	80-81	0.45168047342210116
1106	82-83	0.43666967581575733
1106	84-85	0.37721729483470057
1106	86-87	0.32470576946019136
1106	88-89	0.22048248508853874
1106	90-91	0.244943519075683
1106	92-93	0.21834007130692257
1106	94-95	0.18687331321251932
1106	96-97	0.18919748471497755
1106	98-99	0.260474023213753
1106	100	0.28792295035720983
1107	1	0.06782427026354299
1107	2	-0.07870799784750204
1107	3	0.09816089915862847
1107	4	0.057648833360325114
1107	5	-0.008892267558344713
1107	6	0.010773509292114625
1107	7	-0.020102510328989354
1107	8	0.05932809598620281
1107	9	-0.07822471942581188
1107	10-11	0.06164899322815387
1107	12-13	0.11092874969800448
1107	14-15	0.22017605278475116
1107	16-17	0.22598928895492776
1107	18-19	0.04265792342039276
1107	20-21	0.07954031792505845
1107	22-23	0.008583666883936303
1107	24-25	0.19469674555312366
1107	26-27	0.210244441923912
1107	28-29	0.26937542985549356
1107	30-31	0.2855740181867503
1107	32-33	0.24062975059256075
1107	34-35	0.28726445943406986
1107	36-37	0.17533440314863924
1107	38-39	0.23804443748912973
1107	40-41	0.3700461222592537
1107	42-43	0.291757919416888
1107	44-45	0.18768202203701634
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2216	32-33	-0.15505017304727176
2216	34-35	-0.22041546420577163
2216	36-37	-0.23451383102253942
2216	38-39	-0.20484865827924637
2216	40-41	-0.29859721213463786
2216	42-43	-0.1854673430711813
2216	44-45	-0.12541851884748922
2216	46-47	-0.12445388200753627
2216	48-49	-0.1203385279542033
2216	50-51	-0.13618591142044778
2216	52-53	-0.2431741335652191
2216	54-55	-0.35621237881061774
2216	56-57	-0.2004775090112787
2216	58-59	-0.29849911674470064
2216	60-61	-0.35860440393199866
2216	62-63	-0.5053822076999737
2216	64-65	-0.5530757930918782
2216	66-67	-0.4727165716316506
2216	68-69	-0.45938623264341416
2216	70-71	-0.5118008281627482
2216	72-73	-0.4620110905570094
2216	74-75	-0.583860728260639
2216	76-77	-0.502642172082048
2216	78-79	-0.4930836719929417
2216	80-81	-0.5939653071589035
2216	82-83	-0.8076051734084935
2216	84-85	-0.8490419013349069
2216	86-87	-0.8252689094219967
2216	88-89	-0.9141383887202146
2216	90-91	-0.9760710290574615
2216	92-93	-0.8228051337941302
2216	94-95	-0.7867212266337127
2216	96-97	-0.8344520710145069
2216	98-99	-0.8414786493151354
2216	100	-0.559083909363796
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	70.0
6	455.0
7	1360.0
8	2988.0
9	5403.0
10	7588.0
11	8946.0
12	9182.0
13	9299.0
14	9634.0
15	10087.0
16	10600.0
17	11518.0
18	13030.0
19	14410.0
20	16041.0
21	17897.0
22	19927.0
23	23602.0
24	34984.0
25	47906.0
26	38977.0
27	83071.0
28	56327.0
29	98607.0
30	89629.0
31	107740.0
32	133674.0
33	220218.0
34	245670.0
35	574134.0
36	147868.0
37	14456.0
38	832.0
39	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.814962400319857	32.57274241318707	24.03714041081114	26.57515477568193
2	14.305887834384615	35.64155205943103	25.298739372336943	24.753820733847416
3	12.656457315529924	38.77984384946352	21.72877664184313	26.83492219316343
4	11.594522460061201	37.98576545207728	21.556309294638268	28.86340279322325
5	11.704627378496323	38.05081219753503	22.236591838143376	28.00796858582527
6	11.345821612987963	34.504169470921084	23.718623508951563	30.431385407139395
7	16.420428072637748	38.13745453429892	19.556866399384905	25.885250993678422
8	13.443057973570985	36.61518211171359	18.118083091105706	31.823676823609716
9	10.526931365857026	34.894583048923586	19.07307668709304	35.50540889812635
10-11	17.68745765295447	45.59857960789183	17.27054821853005	19.443414520623655
12-13	17.563488299132654	47.49063065399317	15.320693234537671	19.6251878123365
14-15	19.39032106529638	46.51578483160609	14.520031828509525	19.573862274588002
16-17	19.466307861626778	46.41213334969495	15.208557826155314	18.91300096252296
18-19	20.416712703640208	44.2838616220849	16.511930506659166	18.787495167615727
20-21	19.029675643002626	44.0908278204899	17.65345805197899	19.226038484528488
22-23	19.543220265253694	45.29917315836058	18.36446941711727	16.793137159268458
24-25	19.551723906524423	45.34498742205895	17.52372264873032	17.57956602268631
26-27	21.999125679707333	45.63822505311113	15.712647733286294	16.65000153389525
28-29	21.131348408967153	46.9208490877158	16.76660154003083	15.181200963286217
30-31	21.595295348147737	46.37294628654478	16.591509739882333	15.440248625425145
32-33	22.40642280288679	46.613830366524276	15.711545246075145	15.268201584513793
34-35	22.49961772435985	46.57294082852433	15.55979294321565	15.367648503900169
36-37	23.199107654727186	46.18370923425885	15.25162161799416	15.365561493019806
38-39	23.779774780010857	46.413916328029785	15.163534350422362	14.642774541536992
40-41	24.21773194453066	46.67117095200923	15.101395553508201	14.009701549951908
42-43	24.598576866762873	47.16357212242618	15.204718308154435	13.033132702656516
44-45	24.855309982245092	47.0370542579178	14.98321816292331	13.12441759691379
46-47	24.859521713527116	46.49177574380691	15.386497738805897	13.262204803860076
48-49	24.56204432045327	46.558262270093046	15.986544720708284	12.893148688745404
50-51	24.81696315583609	46.164758564887606	16.492178092908034	12.526100186368271
52-53	24.923802427249502	46.09808749305252	16.859885863621624	12.118224216076353
54-55	24.867916605550235	46.1858073744285	17.01033275140903	11.93594326861223
56-57	24.549781502712012	46.34654271452412	16.55548666307318	12.54818911969069
58-59	24.212202257763536	46.128485108783785	16.590782336802754	13.06853029664993
60-61	24.178599093467906	45.57394525527852	17.051115141846964	13.196340509406612
62-63	24.365325656172885	44.71296450751323	17.416335440525973	13.505374395787918
64-65	24.125630934862116	44.49498847180745	17.50375085682512	13.875629736505305
66-67	23.503617480578825	44.204397539149724	18.035106590269002	14.256878390002441
68-69	23.032146903311947	44.400230372279395	17.674888918235336	14.892733806173316
70-71	22.287421306319295	44.60906793750954	17.919737778407317	15.183772977763846
72-73	22.038065765209986	45.440851432211716	17.88017710848398	14.640905694094322
74-75	21.47302887160747	46.52711684896518	17.68448572022273	14.315368559204614
76-77	20.52980465769079	47.82449678455975	17.707522442890944	13.938176114858516
78-79	19.780818962934557	49.09722338729972	17.30808776828406	13.813869881481658
80-81	19.007534150541602	50.900059558975975	16.565496298835065	13.526909991647361
82-83	18.27203723543214	52.41759479624291	16.179988831287734	13.130379137037224
84-85	17.479517547896247	54.00699653337014	15.915891084932433	12.597594833801177
86-87	16.823857427967518	55.12608216198234	15.521823714821423	12.52823669522872
88-89	16.39351020316207	55.81928484152995	15.309848156930869	12.47735679837712
90-91	16.204296673524524	56.3943672909041	14.889544102787305	12.511791932784067
92-93	16.226475509987885	56.641238974449436	14.427538986385144	12.704746529177536
94-95	16.33097335800362	56.6788580084173	13.968785052104804	13.021383581474275
96-97	16.504843897810424	56.28589842254856	13.754316199237698	13.454941480403312
98-99	16.82057189038468	55.53930580990721	13.550202726362192	14.08991957334591
100	17.531908815111265	54.184851827192205	13.118864680355808	15.164374677340728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245494.0
1	240439.5
2	148902.0
3	37979.5
4	12864.5
5	11473.0
6	9397.0
7	6314.0
8	3542.5
9	2051.5
10	1491.0
11	1357.5
12	1413.0
13	1526.0
14	1585.5
15	1660.0
16	1655.0
17	1618.5
18	1665.5
19	1736.5
20	1807.5
21	1840.0
22	1844.5
23	1830.0
24	1823.0
25	1842.0
26	1882.5
27	1888.0
28	1919.5
29	1946.5
30	1919.0
31	1963.5
32	2094.0
33	2349.0
34	2733.5
35	3251.5
36	3967.5
37	4937.0
38	6256.5
39	8006.5
40	10626.0
41	14313.5
42	19627.0
43	27218.5
44	37164.0
45	49146.0
46	61814.0
47	73367.0
48	82650.5
49	89889.0
50	95886.0
51	102457.0
52	109100.0
53	113690.0
54	113340.5
55	105003.5
56	89178.5
57	68890.0
58	48223.0
59	30818.0
60	18714.0
61	11325.5
62	7076.5
63	4792.0
64	3623.5
65	2890.5
66	2392.0
67	2068.0
68	1828.0
69	1640.0
70	1420.5
71	1233.5
72	1105.5
73	1026.5
74	955.5
75	871.0
76	837.0
77	811.0
78	742.5
79	711.0
80	669.5
81	614.5
82	615.5
83	614.0
84	583.0
85	551.5
86	527.0
87	522.0
88	544.0
89	518.0
90	476.5
91	493.5
92	486.0
93	463.5
94	442.5
95	407.5
96	397.0
97	381.0
98	332.0
99	276.5
100	208.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012942241174350203
2	4.314080391450068E-4
3	0.0
4	0.0
5	0.0
6	0.003643001219446724
7	1.9173690628666968E-4
8	0.0
9	0.0
10-11	3.5950669928750566E-4
12-13	2.396711328583371E-5
14-15	5.75210718860009E-4
16-17	3.8347381257333935E-4
18-19	3.1157247271583824E-4
20-21	1.4380267971500225E-4
22-23	2.396711328583371E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	7.190133985750112E-5
32-33	0.0
34-35	4.793422657166742E-5
36-37	1.9173690628666968E-4
38-39	4.553751524308405E-4
40-41	0.0014619939104358562
42-43	9.82651644719182E-4
44-45	3.8347381257333935E-4
46-47	7.42980511860845E-4
48-49	0.0010545529845766833
50-51	0.0
52-53	1.6776979300083596E-4
54-55	4.793422657166742E-4
56-57	5.033093790025078E-4
58-59	5.991778321458427E-4
60-61	0.0
62-63	1.9173690628666968E-4
64-65	9.586845314333484E-5
66-67	1.1983556642916855E-4
68-69	1.6776979300083596E-4
70-71	8.388489650041799E-4
72-73	6.231449454316765E-4
74-75	3.5950669928750566E-4
76-77	3.8347381257333935E-4
78-79	8.148818517183462E-4
80-81	0.0011743885510058518
82-83	3.355395860016719E-4
84-85	7.669476251466787E-4
86-87	0.0015578623635791912
88-89	0.0011983556642916854
90-91	9.586845314333484E-4
92-93	7.190133985750113E-4
94-95	5.75210718860009E-4
96-97	5.033093790025078E-4
98-99	2.636382461441708E-4
100	6.231449454316765E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2086192.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.93541725727238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.4286963613086	70.805093428401
2	1.2415317235482746	1.7862020514317134
3	0.12602047500095423	0.2719600635645991
4	0.05403946779921683	0.15549406657990386
5	0.03131155948584943	0.11262050482952417
6	0.02230550078455483	0.09627333036416208
7	0.012971014404056905	0.06531527333841465
8	0.007856029060709487	0.04521013827739155
9	0.007051526070504407	0.04565290231640419
>10	0.047308704002503664	0.671850276520326
>50	0.005328366318952475	0.26932715711346117
>100	0.008788742293322173	1.4359724255664787
>500	0.0017974932147887656	0.9180064232457213
>1k	0.003395264961267669	5.329216692513113
>5k	9.320335187793599E-4	4.984742791818947
>10k+	6.657382276995428E-4	13.007062474118857
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	113217	5.4269693297644706	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29350	1.4068695498784387	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26083	1.2502684316688013	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23482	1.1255915083558945	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17573	0.8423481635439116	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13431	0.6438045970840651	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12944	0.620460628743663	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12418	0.5952472255669661	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12284	0.5888240392063626	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10820	0.5186483315054415	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9866	0.4729190793560708	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9846	0.4719603948246374	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9148	0.4385023046776136	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9129	0.4375915543727519	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8616	0.4130012961414865	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7251	0.34757107687116046	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7124	0.3414834300965587	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6978	0.33448503301709526	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6773	0.32465851656990347	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6603	0.31650969805272	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5953	0.28535245078113614	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5788	0.277443303396811	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5696	0.2730333545522176	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5316	0.254818348454984	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4855	0.23272067000544533	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4781	0.22917353723914194	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4373	0.20961637279790163	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4294	0.2058295688987399	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4213	0.20194689654643486	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4189	0.20079647510871484	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3492	0.16738631918826263	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3088	0.14802089165330898	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3053	0.14634319372330062	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3025	0.14500103537929396	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2999	0.1437547454884306	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2895	0.13876958592497718	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2778	0.1331612814160921	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2674	0.12817612185263869	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2547	0.12208847507803693	No Hit
GAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2493	0.11950002684316686	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2421	0.11604876253000682	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2420	0.11600082830343515	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2357	0.1129809720294201	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2180	0.10449661392623498	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2141	0.10262717908993994	No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2091	0.10023046776135658	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003547132766303389	0.0	0.0	0.0	0.0
2	0.009011634595473475	0.0	0.0	0.0	0.0
3	0.01505134714350357	0.0	0.0	0.0	0.0
4	0.022960494527828695	0.0	0.0	0.0	0.0
5	0.037963907444760595	0.0	0.0	0.0	0.0
6	0.06888148358348609	0.0	0.0	0.0	0.0
7	0.10579083804367	0.0	0.0	0.0	0.0
8	0.22404457499597352	0.0	0.0	0.0	0.0
9	0.6543980611564036	0.0	0.0	0.0	0.0
10-11	1.623915727794949	0.0	0.0	0.0	0.0
12-13	2.9207043263515535	0.0	0.0	0.0	0.0
14-15	4.421692730103461	0.0	0.0	0.0	0.0
16-17	6.0706061570555345	0.0	0.0	0.0	0.0
18-19	7.8904530359621745	0.0	0.0	0.0	0.0
20-21	10.230386273171405	0.0	0.0	0.0	0.0
22-23	12.867655517804689	0.0	0.0	0.0	0.0
24-25	15.508927270356708	0.0	0.0	0.0	0.0
26-27	18.044599921771344	0.0	0.0	0.0	0.0
28-29	20.577420486704963	0.0	0.0	0.0	0.0
30-31	23.179218403675215	0.0	0.0	0.0	0.0
32-33	26.083025915160253	0.0	0.0	0.0	0.0
34-35	28.77309471036223	0.0	0.0	0.0	0.0
36-37	31.011311518786382	0.0	0.0	0.0	0.0
38-39	32.79108538427911	0.0	0.0	0.0	0.0
40-41	34.346383266736716	0.0	0.0	0.0	0.0
42-43	35.64341153642618	0.0	0.0	0.0	0.0
44-45	36.72928953806745	0.0	0.0	0.0	0.0
46-47	37.66798549702041	0.0	0.0	0.0	0.0
48-49	38.53075364108385	0.0	0.0	0.0	0.0
50-51	39.39843504337089	0.0	0.0	0.0	0.0
52-53	40.320306088797196	0.0	0.0	0.0	0.0
54-55	41.272231894283934	0.0	0.0	0.0	0.0
56-57	42.26396707493845	0.0	0.0	0.0	0.0
58-59	43.292851281186	0.0	0.0	0.0	0.0
60-61	44.329548766364745	0.0	0.0	0.0	0.0
62-63	45.32722779111414	0.0	0.0	0.0	0.0
64-65	46.250321159318034	0.0	0.0	0.0	0.0
66-67	47.08363372115318	0.0	0.0	0.0	0.0
68-69	47.8163323414144	0.0	0.0	0.0	0.0
70-71	48.46718326980451	0.0	0.0	0.0	0.0
72-73	49.082011626926	0.0	0.0	0.0	0.0
74-75	49.65321983786727	0.0	0.0	0.0	0.0
76-77	50.19763281615498	0.0	0.0	0.0	0.0
78-79	50.715250561789134	0.0	0.0	0.0	0.0
80-81	51.210051615575175	0.0	0.0	0.0	0.0
82-83	51.70049065474319	0.0	0.0	0.0	0.0
84-85	52.1899230751532	0.0	0.0	0.0	0.0
86-87	52.67868441639121	0.0	0.0	0.0	0.0
88	53.036105976822846	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTTGT	35	2.9373055E-4	53.71711	2
TTGCTGA	1400	0.0	49.685944	9
GATAAAA	375	0.0	48.89194	1
TGAAAAA	1175	0.0	48.81192	1
GCCTGCT	370	0.0	48.270493	7
CGTATGC	40	5.6763197E-4	47.00247	2
CCTGCTG	940	0.0	44.000206	8
TGCCAGT	65	2.7845563E-6	43.387936	6
ATTAGCC	65	2.7850147E-6	43.386898	3
CGCCTGC	225	0.0	41.780975	6
ATGTGAC	45	0.0010138841	41.779972	3
ACTGCCG	125	3.6379788E-12	41.362175	4
TTTGCTG	410	0.0	41.268482	8
TACCCGA	2015	0.0	40.355442	6
ATGTGCC	105	1.1405064E-9	40.28783	3
GCATCCG	70	4.6353944E-6	40.28783	2
GCTTGCT	315	0.0	40.2859	7
CTTGCTG	1010	0.0	39.554638	8
ATACGCC	155	0.0	39.421425	3
GTACATT	60	8.813862E-5	39.17624	1
>>END_MODULE