##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248473_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2086192
Sequences flagged as poor quality	0
Sequence length	100
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.976581733608413	33.0	31.0	34.0	30.0	34.0
2	32.26265703252625	34.0	31.0	34.0	30.0	34.0
3	32.45003671761756	34.0	31.0	34.0	30.0	34.0
4	36.04313696917637	37.0	35.0	37.0	35.0	37.0
5	35.95837439698743	37.0	35.0	37.0	35.0	37.0
6	35.949167190747545	37.0	35.0	37.0	35.0	37.0
7	35.95106107203939	37.0	35.0	37.0	35.0	37.0
8	35.94808962933421	37.0	35.0	37.0	35.0	37.0
9	37.69756283218419	39.0	37.0	39.0	35.0	39.0
10-11	37.77086121507512	39.0	37.5	39.0	35.0	39.0
12-13	37.7653394318452	39.0	38.0	39.0	35.0	39.0
14-15	39.23234462599799	41.0	39.0	41.0	36.0	41.0
16-17	39.093834124567636	41.0	39.0	41.0	36.0	41.0
18-19	38.757850667627906	40.0	38.0	41.0	35.0	41.0
20-21	38.23496782654712	40.0	36.5	41.0	34.0	41.0
22-23	37.78872150789572	39.0	35.5	41.0	34.0	41.0
24-25	37.365972547109756	39.0	35.0	41.0	33.0	41.0
26-27	37.13270542692139	38.0	35.0	41.0	33.0	41.0
28-29	36.94763545253744	38.0	35.0	41.0	33.0	41.0
30-31	36.853908700637334	38.0	35.0	41.0	33.0	41.0
32-33	36.852729518663665	38.0	35.0	41.0	33.0	41.0
34-35	36.791482759017384	38.0	35.0	41.0	33.0	41.0
36-37	36.75782502281669	38.0	35.0	41.0	33.0	41.0
38-39	36.64308702171229	38.0	35.0	41.0	33.0	41.0
40-41	36.58230426537922	38.0	35.0	41.0	32.0	41.0
42-43	36.49173613933905	38.0	35.0	41.0	32.0	41.0
44-45	36.28920085016144	37.0	35.0	40.0	32.0	41.0
46-47	36.00688814835834	36.5	35.0	40.0	31.5	41.0
48-49	35.631924818041675	35.5	35.0	39.5	31.0	41.0
50-51	35.205712849057036	35.0	35.0	39.0	31.0	41.0
52-53	34.76781475530536	35.0	35.0	37.5	30.0	40.0
54-55	34.353359614071955	35.0	34.5	37.0	30.0	39.0
56-57	33.95076675588824	35.0	34.0	36.0	29.5	38.5
58-59	33.64299618635293	35.0	34.0	35.0	29.0	37.0
60-61	33.395271144746026	35.0	33.5	35.0	29.0	36.5
62-63	33.19022913518985	35.0	33.0	35.0	29.0	36.0
64-65	33.04045025577703	35.0	33.0	35.0	28.5	35.0
66-67	32.92191634327042	35.0	33.0	35.0	29.0	35.0
68-69	32.82903802718062	35.0	33.0	35.0	28.5	35.0
70-71	32.75178674829546	35.0	33.0	35.0	28.0	35.0
72-73	32.67177134223504	35.0	33.0	35.0	27.0	35.0
74-75	32.61071488146824	35.0	33.0	35.0	27.0	35.0
76-77	32.55564037250646	35.0	33.0	35.0	27.0	35.0
78-79	32.503169171389786	35.0	33.0	35.0	27.0	35.0
80-81	32.4578511949044	35.0	33.0	35.0	27.0	35.0
82-83	32.377469571352975	35.0	33.0	35.0	27.0	35.0
84-85	32.26304649811715	35.0	33.0	35.0	26.5	35.0
86-87	32.16782323966346	35.0	33.0	35.0	25.0	35.0
88-89	32.04900699456235	35.0	33.0	35.0	25.0	35.0
90-91	31.928745292858952	35.0	33.0	35.0	25.0	35.0
92-93	31.72699444729919	35.0	32.5	35.0	24.0	35.0
94-95	31.44000719972083	35.0	32.0	35.0	23.5	35.0
96-97	31.12699070842952	35.0	31.5	35.0	20.0	35.0
98-99	30.64082236917791	34.5	31.0	35.0	18.0	35.0
100	28.96763337219201	33.0	27.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.40486537278611223
1101	2	0.41526901919223747
1101	3	0.34640487431127553
1101	4	0.27694610657921004
1101	5	0.292568623444339
1101	6	0.30343555742838646
1101	7	0.3510191072151585
1101	8	0.3596858940160672
1101	9	0.4408786598837935
1101	10-11	0.3965824303206844
1101	12-13	0.41355002744735003
1101	14-15	0.5296595792331047
1101	16-17	0.5388066520685797
1101	18-19	0.516097756195883
1101	20-21	0.574361774876003
1101	22-23	0.5810305458628804
1101	24-25	0.581355845976276
1101	26-27	0.5970951779320757
1101	28-29	0.684665281531629
1101	30-31	0.7253681486813974
1101	32-33	0.7181524482240249
1101	34-35	0.8106070561969787
1101	36-37	0.7913740529519089
1101	38-39	0.6789006088504479
1101	40-41	0.7052717160475197
1101	42-43	0.6559028311964497
1101	44-45	0.6479487264446817
1101	46-47	0.7015457304648933
1101	48-49	0.6532081041481135
1101	50-51	0.6684068260195204
1101	52-53	0.6310763719513872
1101	54-55	0.6289854296140547
1101	56-57	0.6672225580844113
1101	58-59	0.631587109651683
1101	60-61	0.629191722543446
1101	62-63	0.6496677492296001
1101	64-65	0.5985216128412176
1101	66-67	0.6542542725471421
1101	68-69	0.5959381540768831
1101	70-71	0.6612849206215898
1101	72-73	0.6811040926581171
1101	74-75	0.6375633523382547
1101	76-77	0.5448432090150916
1101	78-79	0.5924388166713044
1101	80-81	0.5360522576197084
1101	82-83	0.5893363420080036
1101	84-85	0.555527596489469
1101	86-87	0.7233356885383486
1101	88-89	0.6903279945440701
1101	90-91	0.6079106182848477
1101	92-93	0.6430602629722735
1101	94-95	0.5808103295397231
1101	96-97	0.6889000674934813
1101	98-99	0.7849619828294188
1101	100	1.266140652240317
1102	1	0.2654835215879885
1102	2	0.3463113155417403
1102	3	0.3133632580061345
1102	4	0.1841703467553657
1102	5	0.2098022993670483
1102	6	0.20614703786610988
1102	7	0.21756406794495575
1102	8	0.26937123465909707
1102	9	0.3441284651176204
1102	10-11	0.3212552048126298
1102	12-13	0.33886184979336065
1102	14-15	0.42579013727289805
1102	16-17	0.4362937623987051
1102	18-19	0.4422712911534674
1102	20-21	0.4580337158158798
1102	22-23	0.45249115856294253
1102	24-25	0.42334677801951415
1102	26-27	0.44703157439899144
1102	28-29	0.4205914280848546
1102	30-31	0.5099689422061999
1102	32-33	0.5167783709074953
1102	34-35	0.5327972758177779
1102	36-37	0.5021249361451225
1102	38-39	0.5199748169890768
1102	40-41	0.5653688293168244
1102	42-43	0.5805205943953666
1102	44-45	0.6473899688825924
1102	46-47	0.5953859850047607
1102	48-49	0.5955647827024606
1102	50-51	0.5089806933500967
1102	52-53	0.4910821926701132
1102	54-55	0.4118765507389668
1102	56-57	0.4187319631818127
1102	58-59	0.45187595561429816
1102	60-61	0.5324755823872493
1102	62-63	0.493892663687042
1102	64-65	0.43041155462879743
1102	66-67	0.5073236931315321
1102	68-69	0.42255526342728444
1102	70-71	0.4308673691899614
1102	72-73	0.48601479361200717
1102	74-75	0.5277758740611631
1102	76-77	0.508475898087589
1102	78-79	0.4798863660229813
1102	80-81	0.4438826698533873
1102	82-83	0.45026464971917335
1102	84-85	0.5104952210337288
1102	86-87	0.5408798639164516
1102	88-89	0.5258249744240899
1102	90-91	0.5105346045707932
1102	92-93	0.47758799626076254
1102	94-95	0.49134860718329065
1102	96-97	0.6403049249382669
1102	98-99	0.77753689904198
1102	100	1.0710048555536709
1103	1	0.1754039109887131
1103	2	0.20982795787891462
1103	3	0.21076733375946333
1103	4	0.1129896737147007
1103	5	0.11988147493572399
1103	6	0.11799098755724202
1103	7	0.17705184358517556
1103	8	0.20230063402684806
1103	9	0.2344330832090975
1103	10-11	0.2456433826144675
1103	12-13	0.27965672319945867
1103	14-15	0.33966761332204953
1103	16-17	0.3774493486749364
1103	18-19	0.3996969009213487
1103	20-21	0.40971583412487433
1103	22-23	0.32063568056099
1103	24-25	0.33969909479912985
1103	26-27	0.34042116363342956
1103	28-29	0.3385178305751637
1103	30-31	0.4082968537693148
1103	32-33	0.4397843598287139
1103	34-35	0.3258132221059995
1103	36-37	0.3300545046914465
1103	38-39	0.45202823391273483
1103	40-41	0.429059483892857
1103	42-43	0.4355282799359088
1103	44-45	0.4422641000298171
1103	46-47	0.43043120401849677
1103	48-49	0.40610774922443227
1103	50-51	0.4201781392371231
1103	52-53	0.4155405795766498
1103	54-55	0.47309804415066736
1103	56-57	0.4048711633055504
1103	58-59	0.38731088953797865
1103	60-61	0.3336605573971525
1103	62-63	0.3564621477407499
1103	64-65	0.38458373973914917
1103	66-67	0.3835554339306384
1103	68-69	0.3377630872108881
1103	70-71	0.2896123300855393
1103	72-73	0.33981599429646536
1103	74-75	0.3730522876689264
1103	76-77	0.3084203108446246
1103	78-79	0.31398225160026527
1103	80-81	0.32079015818006695
1103	82-83	0.27804183671465665
1103	84-85	0.2794377824938792
1103	86-87	0.31410614749839283
1103	88-89	0.36494014635784566
1103	90-91	0.2062868933151094
1103	92-93	0.1316626620122534
1103	94-95	0.15206570678596165
1103	96-97	0.1661544430210391
1103	98-99	0.25261206266182157
1103	100	0.7029230504667154
1104	1	0.1513308516279821
1104	2	0.14589188481955517
1104	3	0.10888148901060646
1104	4	0.07975679700236782
1104	5	0.07680841557499463
1104	6	0.03389509714627792
1104	7	0.12007924084544896
1104	8	0.09893533722319603
1104	9	0.13371162202188458
1104	10-11	0.15429178444095015
1104	12-13	0.1320288693181766
1104	14-15	0.18885482793392327
1104	16-17	0.2215338235607831
1104	18-19	0.18087726621815392
1104	20-21	0.25793044599701886
1104	22-23	0.2183594248532259
1104	24-25	0.1884913322420516
1104	26-27	0.17823623212657935
1104	28-29	0.22579408628293152
1104	30-31	0.26369183093827075
1104	32-33	0.2585514831163849
1104	34-35	0.31577669242562934
1104	36-37	0.29709788368687384
1104	38-39	0.3276218412186722
1104	40-41	0.2610708994179731
1104	42-43	0.22716526623727873
1104	44-45	0.19204263884259376
1104	46-47	0.19154535926963945
1104	48-49	0.1757949638363101
1104	50-51	0.17895211183986248
1104	52-53	0.17078943802413704
1104	54-55	0.14065283867122247
1104	56-57	0.14006294412747167
1104	58-59	0.13426751054254993
1104	60-61	0.07185690442911863
1104	62-63	0.05426351760376491
1104	64-65	0.03251752969348587
1104	66-67	0.0564595435196793
1104	68-69	0.051696877165227306
1104	70-71	-0.04602465621582752
1104	72-73	0.026605948634362164
1104	74-75	0.05524635159586211
1104	76-77	0.06704588162087788
1104	78-79	0.06076992283314553
1104	80-81	0.12610979288326263
1104	82-83	0.15103270429456472
1104	84-85	0.11113412952584412
1104	86-87	-0.02344864702214977
1104	88-89	0.030650192019940903
1104	90-91	0.09933712162560937
1104	92-93	-0.049380716983179695
1104	94-95	-0.013721964446915536
1104	96-97	0.1488622055781157
1104	98-99	0.3198951676846491
1104	100	0.605653644074021
1105	1	0.24327251287283502
1105	2	0.24378019538125528
1105	3	0.1765396976421414
1105	4	0.14962927585727925
1105	5	0.11245729868061005
1105	6	0.12090941074419703
1105	7	0.1477929688870887
1105	8	0.13391755366214397
1105	9	0.1626645606469097
1105	10-11	0.19965103406142504
1105	12-13	0.2216335103938647
1105	14-15	0.24167026926986068
1105	16-17	0.3153235654822808
1105	18-19	0.26998938940185013
1105	20-21	0.28914471622039883
1105	22-23	0.281173131207602
1105	24-25	0.29340360723662684
1105	26-27	0.292753473505897
1105	28-29	0.3235805751139793
1105	30-31	0.32686988342146606
1105	32-33	0.336532940500895
1105	34-35	0.3711170741215781
1105	36-37	0.31770512723491606
1105	38-39	0.3638379548599602
1105	40-41	0.2390475856031813
1105	42-43	0.27373293051834935
1105	44-45	0.26751899971876014
1105	46-47	0.27884019769948054
1105	48-49	0.2991156101169423
1105	50-51	0.26878978262710973
1105	52-53	0.2842213399976785
1105	54-55	0.3198746994389481
1105	56-57	0.28873828570196736
1105	58-59	0.31479527024760046
1105	60-61	0.2663895437698258
1105	62-63	0.3296860940513895
1105	64-65	0.3501921122135485
1105	66-67	0.30024256238760216
1105	68-69	0.3070881155083214
1105	70-71	0.3021640230294551
1105	72-73	0.25391390786229096
1105	74-75	0.23129341297083528
1105	76-77	0.28746639777789795
1105	78-79	0.2989403847719956
1105	80-81	0.28540333007692453
1105	82-83	0.26795636510567533
1105	84-85	0.2562054806427412
1105	86-87	0.21266944016066702
1105	88-89	0.2580234299292883
1105	90-91	0.39990760525171964
1105	92-93	0.3214681227478984
1105	94-95	0.3016879249480091
1105	96-97	0.3996002233616771
1105	98-99	0.5158472388188962
1105	100	0.912981338717266
1106	1	0.15332000191265394
1106	2	0.16469057425987899
1106	3	0.14372604803950395
1106	4	0.058740361294987053
1106	5	0.1760202535812283
1106	6	0.1168080845777979
1106	7	0.12347079740361977
1106	8	0.14527296747349538
1106	9	0.16056069281358987
1106	10-11	0.1637910324804821
1106	12-13	0.18368028192963237
1106	14-15	0.24804571846996737
1106	16-17	0.22709032801883922
1106	18-19	0.30527021927434106
1106	20-21	0.34080196817985353
1106	22-23	0.28634159264783676
1106	24-25	0.17848590738438475
1106	26-27	0.27100033138536617
1106	28-29	0.2783428562904504
1106	30-31	0.30787122722144034
1106	32-33	0.23012496039643082
1106	34-35	0.21189501877647388
1106	36-37	0.21863940264702109
1106	38-39	0.11713816950312861
1106	40-41	-0.004300300496083764
1106	42-43	0.18335782182864335
1106	44-45	0.1339492346101352
1106	46-47	0.16064660537555397
1106	48-49	0.09053322788186335
1106	50-51	0.058223623280397874
1106	52-53	0.06197597793175902
1106	54-55	0.062056641442730154
1106	56-57	0.1356530182492932
1106	58-59	0.09885087073161714
1106	60-61	0.11300632971647673
1106	62-63	0.04477769742974402
1106	64-65	0.13396800772549255
1106	66-67	0.1157072070710754
1106	68-69	0.08280881185208955
1106	70-71	0.023296645750015443
1106	72-73	0.02543750948944279
1106	74-75	0.10811529562852229
1106	76-77	0.12949845439990781
1106	78-79	0.11189528538551485
1106	80-81	0.0574985027339423
1106	82-83	0.09883214569536136
1106	84-85	0.10335735436202498
1106	86-87	0.02023697979061012
1106	88-89	0.12319955285354922
1106	90-91	0.10651077078233584
1106	92-93	0.11352571764997066
1106	94-95	0.09284103909804031
1106	96-97	0.057096924667167315
1106	98-99	0.2550831578017423
1106	100	0.6628855701670489
1107	1	0.07243351362819794
1107	2	0.11438875617388788
1107	3	0.12347561352009961
1107	4	0.058842384021140504
1107	5	0.006324058803336641
1107	6	0.07207486170243982
1107	7	0.12862012535070022
1107	8	0.13627938219084967
1107	9	0.172610550858451
1107	10-11	0.11773525670736973
1107	12-13	0.14204721696903988
1107	14-15	0.15448723858192182
1107	16-17	0.13081004585371403
1107	18-19	0.11923700744204524
1107	20-21	0.20115146625366975
1107	22-23	0.25268183444067205
1107	24-25	0.21503916340123652
1107	26-27	0.29478316181159414
1107	28-29	0.30143270119437204
1107	30-31	0.3800505116042956
1107	32-33	0.28390703513674254
1107	34-35	0.3035278325253259
1107	36-37	0.26597369236223756
1107	38-39	0.32324253270239467
1107	40-41	0.3057860866064317
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2216	52-53	-0.7680915143568114
2216	54-55	-0.6873471613287805
2216	56-57	-0.7417941987296715
2216	58-59	-0.7395658227907873
2216	60-61	-0.8074764289042164
2216	62-63	-0.7890936252533791
2216	64-65	-0.791934851258894
2216	66-67	-0.7674690279088949
2216	68-69	-0.7351126466442963
2216	70-71	-0.7671675133586859
2216	72-73	-0.7679178608894475
2216	74-75	-0.777848886499374
2216	76-77	-0.7951922136172165
2216	78-79	-0.7820872200239961
2216	80-81	-0.8030092547357874
2216	82-83	-0.760157771895912
2216	84-85	-0.7964849180931992
2216	86-87	-0.9209486470221506
2216	88-89	-0.9128259984562455
2216	90-91	-0.9594009736124889
2216	92-93	-0.9688569074593723
2216	94-95	-1.0101505358754892
2216	96-97	-1.0343758896599802
2216	98-99	-1.005200070410588
2216	100	-0.5848225464021688
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	7.0
8	16.0
9	46.0
10	70.0
11	161.0
12	269.0
13	446.0
14	653.0
15	1119.0
16	2030.0
17	3092.0
18	4507.0
19	5894.0
20	7370.0
21	9320.0
22	11128.0
23	13178.0
24	15835.0
25	18705.0
26	22090.0
27	25997.0
28	31699.0
29	40709.0
30	45101.0
31	55400.0
32	72529.0
33	106087.0
34	145229.0
35	581106.0
36	344114.0
37	486119.0
38	34097.0
39	2061.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.052535164473227	22.805189849082456	28.831871652729042	28.31040333371527
2	17.35818400282538	23.00707112585222	36.36409004360912	23.270654827713287
3	13.958398843442982	20.427602061555216	40.13379401320684	25.480205081794967
4	14.698318610328585	16.169612465972673	43.84866965680516	25.283399266893586
5	13.338358714384771	18.89653565191372	45.8869078274146	21.878197806286906
6	11.611576824110042	13.325580681900979	41.484434618880165	33.57840787510881
7	16.667401658140765	13.476851603304011	40.42921265156802	29.4265340869872
8	12.64715807557502	13.08872817075322	40.686092171765594	33.57802158190617
9	11.109955363648217	10.960496445197759	46.551324135074815	31.378224056079212
10-11	15.075026651429974	14.726928298066525	48.35834860837353	21.839696442129966
12-13	13.495585932313315	25.17571665985195	40.31658957966533	21.01210782816941
14-15	16.28595731463113	22.988282602844492	39.019778575251365	21.705981507273012
16-17	24.54301905097901	23.72566858659222	38.91626465828649	12.815047704142286
18-19	24.524156932823058	16.363187089203677	38.25136900151089	20.861286976462377
20-21	17.041501862132456	14.977948967576438	45.73974437052158	22.24080479976953
22-23	16.575762681519365	16.763977910775004	47.93292798232939	18.727331425376246
24-25	16.729226671102314	19.114336327473282	45.150411251836836	19.006025749587575
26-27	21.859558802381432	19.14775614292425	39.832513637627386	19.160171417066927
28-29	19.83984583783732	22.517238982510552	43.06926991081508	14.57364526883705
30-31	20.119883246976553	19.414983752078626	43.10166803044705	17.363464970497763
32-33	21.069496312143666	20.48982708671933	42.40508680417822	16.035589796958778
34-35	20.930924848910465	20.626888244676962	42.49827728569734	15.943909620715235
36-37	21.17444571634559	19.302114551635672	42.23442032945435	17.28901940256438
38-39	21.802557105861403	19.3315189841562	41.2196884448487	17.646235465133696
40-41	21.787437413565407	19.88464202596693	41.38829834405102	16.93962221641664
42-43	21.79056762643262	20.617554970886804	41.44701441434575	16.144862988334825
44-45	22.238459396066617	19.750720366465316	40.904495537347124	17.106324700120943
46-47	22.886141220908367	18.957127286839135	41.32767127188562	16.82906022036688
48-49	21.840970929262678	18.914578703446267	41.72602829032772	17.518422076963343
50-51	21.652530297099855	18.446398323721468	41.84447684486873	18.056594534309948
52-53	20.731257482446587	18.6824183027684	42.481649655522844	18.104674559262175
54-55	20.561374176030533	18.90541814375794	42.84085186872668	17.692355811484845
56-57	20.080284706789207	19.243306571764403	42.25955813690393	18.41685058454246
58-59	19.748625143956925	19.247145728149405	43.08960022817397	17.914628899719695
60-61	18.860966762316803	18.543118427748592	43.84859201098039	18.747322798954215
62-63	18.4930355954195	18.14108201695312	44.32057474644984	19.04530764117754
64-65	18.38400224737092	17.85900048562004	44.76934965745331	18.987647609555736
66-67	17.872140403123492	17.702504366743664	45.471086967412475	18.954268262720365
68-69	17.66873850813512	17.273122333246164	46.04797298544358	19.01016617317514
70-71	17.332004479369207	16.953626256466897	46.465617581524135	19.24875168263976
72-73	16.981681090529865	16.541834556247796	47.14998572673908	19.326498626483264
74-75	16.70990097135175	15.922431833167275	47.97850164911928	19.389165546361692
76-77	16.934912328930626	15.560317585479863	48.376732863463026	19.128037222126487
78-79	17.11962442493904	15.669798456309032	48.468449638286565	18.742127480465363
80-81	17.061915357233854	15.377487446966143	48.52135579716589	19.039241398634115
82-83	17.086156465120606	15.471868795591876	48.43766666434959	19.004308074937924
84-85	16.94534022558824	15.742519341921646	48.39794862256371	18.9141918099264
86-87	16.901410813842563	16.131335599200394	48.25822255672258	18.709031030234463
88-89	16.75303694186905	16.914136281268277	48.0198036414539	18.313023135408777
90-91	16.73120163023587	17.85898756925974	47.63629460732182	17.773516193182566
92-93	16.436575526669657	18.732397404583445	47.0566902446566	17.774336824090298
94-95	16.305601713803206	19.960951440724543	46.47696525680084	17.256481588671413
96-97	15.576111555595396	21.442569088277775	46.4496518082148	16.531667547912026
98-99	15.121904246035328	22.802467575456653	45.97848996419006	16.097138214317955
100	14.638392891998034	24.001315573738495	45.32219845254175	16.038093081721723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62242.0
1	137867.0
2	204514.5
3	135065.0
4	45131.0
5	10887.5
6	4711.5
7	2815.5
8	2102.5
9	1894.5
10	1897.0
11	2049.0
12	2342.0
13	2685.0
14	2955.5
15	3109.0
16	3356.5
17	3684.5
18	3913.5
19	3739.5
20	3166.0
21	2583.5
22	2233.5
23	2087.0
24	2044.5
25	2015.0
26	2014.5
27	2031.0
28	1988.5
29	1912.5
30	1814.5
31	1798.5
32	1795.0
33	1760.5
34	1664.0
35	1599.0
36	1624.5
37	1568.0
38	1533.0
39	1593.0
40	1831.0
41	2336.0
42	3283.5
43	5409.5
44	9635.0
45	16647.5
46	26757.0
47	39354.0
48	54346.5
49	72634.5
50	95744.5
51	123168.0
52	149719.0
53	168193.5
54	171564.5
55	156324.5
56	126316.0
57	90740.5
58	58340.5
59	33988.0
60	18137.5
61	8897.0
62	4142.5
63	1860.0
64	825.5
65	423.5
66	257.0
67	170.5
68	122.5
69	90.0
70	67.0
71	52.0
72	37.0
73	29.5
74	28.0
75	18.5
76	6.5
77	7.5
78	8.0
79	4.5
80	2.5
81	1.5
82	0.5
83	0.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016824913526655265
2	0.10660571989538833
3	0.0
4	4.793422657166742E-5
5	0.037292828272757254
6	0.001150421437720018
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0010066187580050157
14-15	9.82651644719182E-4
16-17	0.0
18-19	0.0
20-21	4.074409258591731E-4
22-23	7.909147384325125E-4
24-25	0.0029479549341575465
26-27	0.002492579781726706
28-29	0.0036909354460183917
30-31	0.0037868038991617265
32-33	0.004242179051592567
34-35	0.004529784411022571
36-37	0.003643001219446724
38-39	0.0021330730824392
40-41	0.0036909354460183917
42-43	0.00529673203616925
44-45	0.0048413568837384095
46-47	0.012534800248491032
48-49	0.005176896469740081
50-51	0.006830627286462607
52-53	0.0028520864810142117
54-55	0.0012223227775775192
56-57	0.005560370282313421
58-59	0.003091757613872549
60-61	0.013972827045641054
62-63	0.012630668701634366
64-65	0.009538911087761818
66-67	0.012247194889061026
68-69	0.009850483560477656
70-71	0.008819897689186805
72-73	0.0059198769816009265
74-75	0.0037628367858758924
76-77	6.471120587175102E-4
78-79	0.010425694279337663
80-81	0.006830627286462607
82-83	0.010809168091911002
84-85	0.008580226556328467
86-87	0.007765344704610122
88-89	0.010162056033193494
90-91	0.005440534715884252
92-93	0.009922384900335156
94-95	0.003427297199874221
96-97	0.006183515227745098
98-99	0.44360729980749614
100	1.7685812235882412
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2086192.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.08625281299085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.23593589115615	70.81460509970643
2	1.360711665815058	1.9617721029506034
3	0.11996887722874974	0.25944320440806945
4	0.05300296490877216	0.1528314051300733
5	0.03341036167360537	0.12042138890884883
6	0.026499610277330193	0.1146154563538413
7	0.018239777712516007	0.09203860592060294
8	0.01451809771876907	0.08372442100151144
9	0.01344739597895273	0.0872435147593665
>10	0.09360270205840329	1.3440875609683887
>50	0.009370990213102974	0.47937752453414756
>100	0.010247409660660374	1.6272694914772652
>500	0.002860861389830529	1.4401003486738402
>1k	0.006253976061489994	11.685785811318919
>5k	0.0018628864864012745	9.175022386620341
>10k+	6.653166022861695E-5	0.5616616772677705
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11711	0.5613577273807971	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9321	0.44679492587451203	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9147	0.4384543704510419	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9065	0.4345237638721652	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8410	0.403126845467723	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8152	0.3907598150122328	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7642	0.3663133594606824	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7568	0.362766226694379	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7548	0.3618075421629457	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7510	0.35998604155322234	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7261	0.3480504191368771	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7256	0.3478107480040188	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6940	0.3326635324073719	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6755	0.32379570049161344	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6685	0.3204403046315967	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6655	0.3190022778344467	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6606	0.316653500732435	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6561	0.31449646053670993	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6310	0.3024649696672214	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6110	0.29287812435288796	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5929	0.28420202934341615	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5844	0.2801276200848244	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5591	0.2680002607621925	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5568	0.2668977735510442	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5492	0.26325477233159744	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5406	0.25913242884643406	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5379	0.25783820472899904	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5339	0.2559208356661324	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5255	0.2518943606341123	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4988	0.23909592213947709	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4914	0.2355487893731737	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4874	0.23363142031030698	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4872	0.23353555185716368	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4716	0.22605781251198356	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4713	0.22591400983226856	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4664	0.22356523273025686	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4646	0.22270241665196686	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4545	0.21786105976822845	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4490	0.2152246773067867	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4352	0.20860975403989662	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4324	0.20726759569588993	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4305	0.20635684539102828	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4289	0.20558989776588155	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4282	0.2052543581798799	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4192	0.20094027778842982	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4158	0.19931051408499315	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4130	0.19796835574098648	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3986	0.19106582711466635	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3966	0.190107142583233	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3921	0.18795010238750798	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3786	0.18147898180033284	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3711	0.1778839148074578	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3702	0.17745250676831278	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3572	0.17122105731399603	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3513	0.16839293794626767	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3479	0.16676317424283096	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3465	0.16609209507082762	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3419	0.16388712064853092	No Hit
CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3381	0.16206562003880756	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3239	0.15525895986563076	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3224	0.15453994646705577	No Hit
CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3115	0.149315115770744	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3077	0.14749361516102066	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2979	0.14279606095699723	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2968	0.1422687844647089	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2905	0.13924892819069384	No Hit
GCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2690	0.12894306947778536	No Hit
ACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2688	0.128847201024642	No Hit
CATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2512	0.12041077714802856	No Hit
CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2501	0.11988350065574023	No Hit
GCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2485	0.11911655303059353	No Hit
AACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2399	0.11499420954543015	No Hit
GCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2371	0.11365205120142347	No Hit
GATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2316	0.11101566873998175	No Hit
GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2313	0.11087186606026675	No Hit
GTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2256	0.1081396151456817	No Hit
CATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2234	0.10708506216110501	No Hit
TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2206	0.10574290381709832	No Hit
AATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2196	0.10526356155138164	No Hit
TTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2130	0.10209990259765162	No Hit
ATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2125	0.10186023146479327	No Hit
CCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2106	0.10094948115993159	No Hit
GTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2092	0.10027840198792826	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002492579781726706	0.0	0.0	0.0	0.0
2	0.006998397079463444	0.0	0.0	0.0	0.0
3	0.01246289890863353	0.0	0.0	0.0	0.0
4	0.019796835574098646	0.0	0.0	0.0	0.0
5	0.03484818271760221	0.0	0.0	0.0	0.0
6	0.06811453595833941	0.0	0.0	0.0	0.0
7	0.10555116691081166	0.0	0.0	0.0	0.0
8	0.2746631182556543	0.0	0.0	0.0	0.0
9	0.9518778712601716	0.0	0.0	0.0	0.0
10-11	2.2669294101405812	0.0	0.0	0.0	0.0
12-13	3.7164604216678043	0.0	0.0	0.0	0.0
14-15	5.2657425586906665	0.0	0.0	0.0	0.0
16-17	6.81531230107296	0.0	0.0	0.0	0.0
18-19	8.483471319993559	0.0	4.793422657166742E-5	0.0	0.0
20-21	10.64350740487932	0.0	4.793422657166742E-5	0.0	0.0
22-23	13.185603242654558	0.0	4.793422657166742E-5	0.0	0.0
24-25	15.98119923765406	0.0	4.793422657166742E-5	0.0	0.0
26-27	18.813992192473176	0.0	4.793422657166742E-5	0.0	0.0
28-29	21.76721989155361	0.0	4.793422657166742E-5	0.0	0.0
30-31	24.7560387538635	0.0	4.793422657166742E-5	0.0	0.0
32-33	28.09446589767385	0.0	4.793422657166742E-5	0.0	0.0
34-35	31.259083535935332	0.0	4.793422657166742E-5	0.0	0.0
36-37	33.73977562947226	0.0	4.793422657166742E-5	0.0	0.0
38-39	35.62131385797664	0.0	4.793422657166742E-5	2.396711328583371E-5	0.0
40-41	37.39686951153106	0.0	4.793422657166742E-5	4.793422657166742E-5	0.0
42-43	38.872069301387405	0.0	4.793422657166742E-5	4.793422657166742E-5	0.0
44-45	40.05657197419988	0.0	4.793422657166742E-5	7.190133985750112E-5	0.0
46-47	41.05271710369899	0.0	4.793422657166742E-5	9.586845314333484E-5	0.0
48-49	41.93885318321612	0.0	4.793422657166742E-5	9.586845314333484E-5	0.0
50-51	42.79639649658324	0.0	4.793422657166742E-5	9.586845314333484E-5	0.0
52-53	43.71841134468927	0.0	4.793422657166742E-5	9.586845314333484E-5	0.0
54-55	44.606656530175556	0.0	4.793422657166742E-5	9.586845314333484E-5	0.0
56-57	45.48411651468321	0.0	4.793422657166742E-5	9.586845314333484E-5	0.0
58-59	46.31723733961208	0.0	4.793422657166742E-5	9.586845314333484E-5	0.0
60-61	47.11706784418692	0.0	4.793422657166742E-5	9.586845314333484E-5	0.0
62-63	47.90390817336085	0.0	4.793422657166742E-5	9.586845314333484E-5	0.0
64-65	48.668291317385936	0.0	4.793422657166742E-5	9.586845314333484E-5	0.0
66-67	49.37771787064662	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
68-69	50.007669476251465	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
70-71	50.599753042864705	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
72-73	51.18852914784449	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
74-75	51.75247532346016	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
76-77	52.29070478652012	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
78-79	52.79017942739691	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
80-81	53.27124732527015	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
82-83	53.74490938513809	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
84-85	54.209200303711256	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
86-87	54.668290358701405	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
88	55.00232001656607	0.0	4.793422657166742E-5	1.4380267971500225E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCATTTT	350	0.0	67.205574	1
GCCTTTT	480	0.0	64.68536	1
ACTTTTC	745	0.0	63.77764	1
CATTTTC	400	0.0	63.50927	1
AACTTTT	460	0.0	63.407	1
CACTTTT	695	0.0	60.24327	1
CCTTTTC	945	0.0	60.23611	1
ACCTTTT	750	0.0	60.216198	1
CATTTCT	605	0.0	59.096478	1
GCATTTT	320	0.0	58.80488	1
CGTTTTT	795	0.0	58.582973	1
GACTTTT	470	0.0	58.054176	1
AATTTTC	440	0.0	56.66652	1
GTTTTTC	880	0.0	56.66652	1
CCGTAAT	25	0.005217321	56.452686	1
TGAACGC	25	0.005221783	56.44049	2
AGCTTTT	245	0.0	55.684624	1
ACATTTT	315	0.0	55.257915	1
ATTTCTT	2410	0.0	55.2305	2
GTCCCTA	485	0.0	54.235413	6
>>END_MODULE