##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248472_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2424673
Sequences flagged as poor quality	0
Sequence length	100
%GC	18
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.191257130342937	31.0	27.0	34.0	10.0	34.0
2	29.207479524043038	31.0	28.0	34.0	15.0	34.0
3	30.440644985942434	33.0	30.0	34.0	25.0	34.0
4	34.48099723137924	37.0	35.0	37.0	31.0	37.0
5	34.323650653098376	37.0	35.0	37.0	31.0	37.0
6	33.948220646660396	37.0	35.0	37.0	28.0	37.0
7	34.42855840766982	37.0	35.0	37.0	31.0	37.0
8	34.22577683671159	37.0	35.0	37.0	30.0	37.0
9	35.59914347213006	39.0	35.0	39.0	29.0	39.0
10-11	36.8215553190059	39.0	37.0	39.0	32.0	39.0
12-13	36.911989781714894	39.0	37.0	39.0	32.0	39.0
14-15	38.45366818535943	41.0	39.0	41.0	32.0	41.0
16-17	38.176744657939444	40.5	39.0	41.0	32.0	41.0
18-19	36.4689290060969	36.5	36.0	40.0	31.5	41.0
20-21	35.40324633466038	35.0	35.0	39.5	30.0	41.0
22-23	34.73852804068837	35.0	35.0	38.0	29.0	41.0
24-25	34.29960143079087	35.0	34.0	38.0	25.0	41.0
26-27	34.139174024703536	35.0	33.5	37.5	25.0	41.0
28-29	33.98622123478094	35.0	33.0	37.0	25.0	40.0
30-31	33.94037195943535	35.0	33.0	37.0	25.0	40.0
32-33	33.896365406799184	35.0	33.0	37.0	25.0	40.0
34-35	33.817441568409436	35.0	33.0	37.0	25.0	40.0
36-37	33.75179663402034	35.0	33.0	37.0	25.0	40.0
38-39	33.62532246616348	35.0	33.0	36.5	25.0	40.0
40-41	33.48332166853015	35.0	33.0	36.0	25.0	40.0
42-43	33.365366587576965	35.0	33.0	35.0	25.0	40.0
44-45	33.26034397215625	35.0	33.0	35.0	25.0	40.0
46-47	33.19740765043369	35.0	33.0	35.0	25.0	40.0
48-49	33.200922557392275	35.0	33.0	35.0	25.0	39.5
50-51	33.11013794437436	35.0	33.0	35.0	25.0	39.0
52-53	32.92553717552841	35.0	33.0	35.0	25.0	39.0
54-55	32.902180005303805	35.0	33.0	35.0	25.5	39.0
56-57	32.86438150628972	35.0	33.0	35.0	26.0	37.5
58-59	32.65224630290353	35.0	33.0	35.0	26.0	37.0
60-61	32.47465513906411	35.0	32.0	35.0	26.0	36.0
62-63	32.28099026136721	35.0	32.0	35.0	26.0	35.5
64-65	32.18089037160887	35.0	31.5	35.0	26.0	35.0
66-67	32.117210856886686	35.0	31.0	35.0	26.0	35.0
68-69	32.02245849234103	35.0	31.0	35.0	25.0	35.0
70-71	31.954895979787793	35.0	31.0	35.0	25.0	35.0
72-73	31.887597832779925	35.0	31.0	35.0	25.0	35.0
74-75	31.787760040219858	35.0	31.0	35.0	25.0	35.0
76-77	31.59749500241888	35.0	30.5	35.0	25.0	35.0
78-79	31.364253653997878	35.0	30.0	35.0	24.0	35.0
80-81	31.118660536905388	35.0	30.0	35.0	24.0	35.0
82-83	30.907419680921922	35.0	30.0	35.0	21.5	35.0
84-85	30.714195481205095	35.0	30.0	35.0	20.0	35.0
86-87	30.495802114346965	35.0	29.5	35.0	18.5	35.0
88-89	30.28711294265247	35.0	29.0	35.0	16.0	35.0
90-91	30.007113742760364	35.0	28.5	35.0	12.5	35.0
92-93	29.714812677833258	35.0	28.0	35.0	10.0	35.0
94-95	29.376648727477892	35.0	28.0	35.0	10.0	35.0
96-97	29.001431739455178	35.0	28.0	35.0	8.0	35.0
98-99	28.528659122281645	34.0	26.0	35.0	8.0	35.0
100	27.87058378593732	33.0	25.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5551313240186921
1101	2	0.27579630469439564
1101	3	0.15617624184436352
1101	4	0.1834074775479877
1101	5	0.14119823073573912
1101	6	0.23968386227083016
1101	7	0.04058478526813758
1101	8	0.2216385427344747
1101	9	0.26607116969808686
1101	10-11	0.24012938243937043
1101	12-13	0.23409032863589374
1101	14-15	0.32350863093849114
1101	16-17	0.395935463081166
1101	18-19	0.3287684940793625
1101	20-21	0.26801865017404225
1101	22-23	0.25484838890039896
1101	24-25	0.26037759171997976
1101	26-27	0.24314096868090473
1101	28-29	0.18060748318433184
1101	30-31	0.20779464173977402
1101	32-33	0.2254348744515866
1101	34-35	0.3074456368393541
1101	36-37	0.18304436458535633
1101	38-39	0.2102271488842078
1101	40-41	0.2296092305430335
1101	42-43	0.18166591193018178
1101	44-45	0.27479961320374713
1101	46-47	0.30445449563534055
1101	48-49	0.2525186834389288
1101	50-51	0.32743407327059515
1101	52-53	0.2804687243362949
1101	54-55	0.25180881230215846
1101	56-57	0.2582751282891209
1101	58-59	0.23337719597063966
1101	60-61	0.24630873797764252
1101	62-63	0.29211622170077334
1101	64-65	0.31597630813736544
1101	66-67	0.36410445838271244
1101	68-69	0.3484580383316285
1101	70-71	0.3302354633581217
1101	72-73	0.3338706382746892
1101	74-75	0.3489896926344329
1101	76-77	0.2659599176435101
1101	78-79	0.27268188330356224
1101	80-81	0.19845053178100258
1101	82-83	0.15737377184452583
1101	84-85	0.09716209898676453
1101	86-87	0.07671957986756794
1101	88-89	0.09343895199329566
1101	90-91	0.12481409332759341
1101	92-93	0.061094211199929305
1101	94-95	0.029161810972997415
1101	96-97	0.06095769769008896
1101	98-99	0.04165674904724881
1101	100	0.1947174458169023
1102	1	0.3883916417556321
1102	2	0.34836174712117085
1102	3	0.18646972434056863
1102	4	0.2247216416993041
1102	5	0.17680280640657742
1102	6	0.13031845875890724
1102	7	0.07980526613790317
1102	8	0.011711416621309922
1102	9	0.12088350051973862
1102	10-11	0.19403995808267638
1102	12-13	0.18041085011901004
1102	14-15	0.33175376514999755
1102	16-17	0.35599864544156645
1102	18-19	0.2566432055471566
1102	20-21	0.20511076409766815
1102	22-23	0.2984182977912013
1102	24-25	0.3618164060069944
1102	26-27	0.27770729573711606
1102	28-29	0.37845341756235484
1102	30-31	0.41544813353856114
1102	32-33	0.41474109965178485
1102	34-35	0.4438568112306456
1102	36-37	0.4125272583828945
1102	38-39	0.42686472489102556
1102	40-41	0.4398809319792889
1102	42-43	0.4720797430238477
1102	44-45	0.47426570989650685
1102	46-47	0.46035590960523365
1102	48-49	0.4276811896236481
1102	50-51	0.41436564836022427
1102	52-53	0.4379849739497317
1102	54-55	0.4555414721030502
1102	56-57	0.41521529338979946
1102	58-59	0.45697286182916486
1102	60-61	0.4453170424590098
1102	62-63	0.44052858725634536
1102	64-65	0.4314281872412309
1102	66-67	0.3993262172515415
1102	68-69	0.4831712106567778
1102	70-71	0.4951972434874108
1102	72-73	0.46336286882227284
1102	74-75	0.45718840440413544
1102	76-77	0.4414185063429805
1102	78-79	0.5395602700739595
1102	80-81	0.5595336108662892
1102	82-83	0.533365098013789
1102	84-85	0.5700499457241648
1102	86-87	0.5234486469794994
1102	88-89	0.45706044115165767
1102	90-91	0.4551857974874949
1102	92-93	0.4284801995823564
1102	94-95	0.42831857882531565
1102	96-97	0.575080411530557
1102	98-99	0.4805917906942767
1102	100	0.33612528902864725
1103	1	0.30709976209456613
1103	2	0.2353873293700488
1103	3	0.12440457273703132
1103	4	0.1609879498205089
1103	5	0.10505332004024126
1103	6	0.19312967200337283
1103	7	0.12358617727436894
1103	8	0.1551621987695313
1103	9	0.10482805109932514
1103	10-11	0.28020970185436767
1103	12-13	0.26995280753789075
1103	14-15	0.31387205227438386
1103	16-17	0.27704884697308785
1103	18-19	0.2829731130278148
1103	20-21	0.28127626465552424
1103	22-23	0.29614887823035474
1103	24-25	0.2569634638496936
1103	26-27	0.23822267384109352
1103	28-29	0.28406952760898463
1103	30-31	0.2971937937791509
1103	32-33	0.29153844171090526
1103	34-35	0.31474841125480424
1103	36-37	0.3651305973610093
1103	38-39	0.3626334393945072
1103	40-41	0.33353356504049714
1103	42-43	0.31992825888066534
1103	44-45	0.3507501889116682
1103	46-47	0.32754679213452675
1103	48-49	0.29147768895813897
1103	50-51	0.3158012486895032
1103	52-53	0.2994192422273656
1103	54-55	0.3318193328130974
1103	56-57	0.31702627300855823
1103	58-59	0.34269776799138896
1103	60-61	0.2884524627922218
1103	62-63	0.3201337588857527
1103	64-65	0.28377698035829724
1103	66-67	0.2789674143958436
1103	68-69	0.2832521482933714
1103	70-71	0.29491877142390877
1103	72-73	0.26545625653862714
1103	74-75	0.31751053168871124
1103	76-77	0.21404602891576374
1103	78-79	0.21005339126726952
1103	80-81	0.16064840093241628
1103	82-83	0.18306802137864153
1103	84-85	0.12119448149900336
1103	86-87	0.14317073291223892
1103	88-89	0.1988863833362977
1103	90-91	0.3409200035870654
1103	92-93	0.3818932437120637
1103	94-95	0.46101870854534965
1103	96-97	0.5168740763568671
1103	98-99	0.5414424550245016
1103	100	0.4924982871584227
1104	1	0.29317676842201834
1104	2	0.19618965852448866
1104	3	0.22665001761694015
1104	4	0.15173402905303846
1104	5	0.23083118942100356
1104	6	0.16158713141087588
1104	7	0.21666009165157618
1104	8	0.14254853755497976
1104	9	0.12506949563918823
1104	10-11	0.24977506131750005
1104	12-13	0.284735307286482
1104	14-15	0.3322245055952351
1104	16-17	0.3368598980500721
1104	18-19	0.3709023101718145
1104	20-21	0.3061778266368975
1104	22-23	0.3426212250257521
1104	24-25	0.270496305107244
1104	26-27	0.2130343622849722
1104	28-29	0.30586581536401525
1104	30-31	0.2795959147125089
1104	32-33	0.3288740750985184
1104	34-35	0.3444941800603871
1104	36-37	0.28050989793842973
1104	38-39	0.3009226942274168
1104	40-41	0.28110469916127556
1104	42-43	0.29172619356729257
1104	44-45	0.36001953055053804
1104	46-47	0.3633321255665294
1104	48-49	0.3341623552037376
1104	50-51	0.26827512778654494
1104	52-53	0.29813336871539065
1104	54-55	0.3412941258916504
1104	56-57	0.41313338105172903
1104	58-59	0.4062660510420457
1104	60-61	0.3612183127176962
1104	62-63	0.3374801423244449
1104	64-65	0.3585007130444353
1104	66-67	0.3005980916027511
1104	68-69	0.3778941424977518
1104	70-71	0.40197779981114934
1104	72-73	0.3583896793508785
1104	74-75	0.33615676789958826
1104	76-77	0.26718211430527816
1104	78-79	0.278727293307778
1104	80-81	0.3156555928249354
1104	82-83	0.2868498743243393
1104	84-85	0.30849149281180743
1104	86-87	0.27262737566883644
1104	88-89	0.23078207498946668
1104	90-91	0.18960888440487622
1104	92-93	0.2197476299714296
1104	94-95	0.23336283040186245
1104	96-97	0.3269316211077573
1104	98-99	0.3182156433626169
1104	100	0.29172920068472763
1105	1	0.08570880063299668
1105	2	0.11399255599674873
1105	3	0.055525891870452426
1105	4	0.03943856325625461
1105	5	-0.00490264126834461
1105	6	-0.064431972848503
1105	7	0.10470353919897946
1105	8	0.06175241295964895
1105	9	0.15629313287679736
1105	10-11	0.16029691600159168
1105	12-13	0.14389100636343954
1105	14-15	0.2194759984064234
1105	16-17	0.23620912726313037
1105	18-19	0.17627001155045008
1105	20-21	0.21514468156516386
1105	22-23	0.18987730909193345
1105	24-25	0.21909058238801293
1105	26-27	0.20288770508936693
1105	28-29	0.21175619461866546
1105	30-31	0.18714309022504239
1105	32-33	0.17009354084368766
1105	34-35	0.13058367376304858
1105	36-37	0.13122489892903388
1105	38-39	0.2781587832229562
1105	40-41	0.19908346407611077
1105	42-43	0.22844690724856065
1105	44-45	0.11753136107622453
1105	46-47	0.09599776966766882
1105	48-49	0.15194898483547803
1105	50-51	0.09180976856861633
1105	52-53	0.11996033540501827
1105	54-55	0.11253962344828494
1105	56-57	0.05577416769654775
1105	58-59	0.17685965082278443
1105	60-61	0.12706577819304243
1105	62-63	0.1485124331712271
1105	64-65	0.16737739252339168
1105	66-67	0.11052356496669802
1105	68-69	0.09658847189767528
1105	70-71	0.1965007233380902
1105	72-73	0.19308877901737276
1105	74-75	0.21612611543341842
1105	76-77	0.17111386026106246
1105	78-79	0.17006485606187027
1105	80-81	0.1821915995502792
1105	82-83	0.1069751693626948
1105	84-85	0.10292213391009142
1105	86-87	0.22749073315838686
1105	88-89	0.2434275035221134
1105	90-91	0.23188052500189116
1105	92-93	0.17081948629699895
1105	94-95	0.09651178616492828
1105	96-97	0.1742126132630979
1105	98-99	0.13623273406840752
1105	100	0.17896162451619801
1106	1	0.06406969692011444
1106	2	0.010484383330378932
1106	3	0.11835130511429881
1106	4	0.12252048235948365
1106	5	0.22914865188521816
1106	6	0.2864026133463682
1106	7	0.16484634536826803
1106	8	0.11735252361238224
1106	9	-0.05710226220556791
1106	10-11	0.24493867778550538
1106	12-13	0.17008611599384693
1106	14-15	0.19661109496697549
1106	16-17	0.19834997696383994
1106	18-19	0.16933288715833328
1106	20-21	0.2211586975777351
1106	22-23	0.19829734044354552
1106	24-25	0.22627451047977587
1106	26-27	0.2586545966697429
1106	28-29	0.2513740417815882
1106	30-31	0.23672301076354785
1106	32-33	0.24697854619591197
1106	34-35	0.2657516774835429
1106	36-37	0.24203186203276061
1106	38-39	0.2198230243496937
1106	40-41	0.26249096681236495
1106	42-43	0.2348119249489926
1106	44-45	0.2552923271226817
1106	46-47	0.27302153194069234
1106	48-49	0.22251664136298643
1106	50-51	0.2040772675362561
1106	52-53	0.2222870897118625
1106	54-55	0.17432409919088343
1106	56-57	0.22077430801704878
1106	58-59	0.263092246070336
1106	60-61	0.19248062570476066
1106	62-63	0.19626493989658655
1106	64-65	0.2203725534709946
1106	66-67	0.07116655355262935
1106	68-69	0.10589043758730554
1106	70-71	0.0789748141615263
1106	72-73	0.0928857152196585
1106	74-75	0.12515453634678408
1106	76-77	0.0819544200997342
1106	78-79	0.08401916770967333
1106	80-81	0.04520512243655617
1106	82-83	0.1180191137316342
1106	84-85	0.03199160859026051
1106	86-87	0.0733996691682357
1106	88-89	0.004954791848650331
1106	90-91	-0.07206873510819989
1106	92-93	0.009365765999838516
1106	94-95	0.004319980079831254
1106	96-97	0.0401821677254901
1106	98-99	0.05126987890721324
1106	100	0.10290286631246204
1107	1	-0.316461456840468
1107	2	-0.11620164343808526
1107	3	0.0027736832061577843
1107	4	-0.0036781850722178433
1107	5	-0.033181664238703945
1107	6	0.07829221392717045
1107	7	-0.1544371747353992
1107	8	-0.1015572996876557
1107	9	-0.1164725905076196
1107	10-11	-0.07552655014456633
1107	12-13	-0.031036496683121584
1107	14-15	-0.04613546334557839
1107	16-17	-0.017219252964686405
1107	18-19	-0.10361816222944498
1107	20-21	-0.1262283131991211
1107	22-23	-0.1824256969066198
1107	24-25	-0.1530579563328729
1107	26-27	-0.119294169318934
1107	28-29	-0.0853243582777452
1107	30-31	-0.095208315520523
1107	32-33	-0.12256735012606157
1107	34-35	-0.03825660520517005
1107	36-37	-0.0604705482218435
1107	38-39	-0.08607599086125362
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2216	20-21	-0.02724056427133803
2216	22-23	-0.12436860049457721
2216	24-25	-0.106197069238398
2216	26-27	-0.10745875101471114
2216	28-29	-0.07920028083090358
2216	30-31	-0.09287792026118069
2216	32-33	-0.06747865298225264
2216	34-35	-0.04968185542514192
2216	36-37	-0.1434702718379981
2216	38-39	-0.1951428713121146
2216	40-41	-0.1787095255061928
2216	42-43	-0.23998407831351898
2216	44-45	-0.33538284560561493
2216	46-47	-0.36367068233229105
2216	48-49	-0.28484152692431053
2216	50-51	-0.2877723758923807
2216	52-53	-0.34944326617299737
2216	54-55	-0.40702376178960264
2216	56-57	-0.42800481715314476
2216	58-59	-0.43674129403024864
2216	60-61	-0.4169085037315412
2216	62-63	-0.40543293800121916
2216	64-65	-0.4872073543091737
2216	66-67	-0.5028133319980164
2216	68-69	-0.5559250933305258
2216	70-71	-0.6416781780793812
2216	72-73	-0.6503374675576943
2216	74-75	-0.5339398721108957
2216	76-77	-0.4915448827758837
2216	78-79	-0.5598152615718384
2216	80-81	-0.551622593855889
2216	82-83	-0.4765668205376272
2216	84-85	-0.4542141877578878
2216	86-87	-0.3596532125788734
2216	88-89	-0.35416387294158014
2216	90-91	-0.374137617208369
2216	92-93	-0.43743630461485594
2216	94-95	-0.37190757177951994
2216	96-97	-0.3303202685321658
2216	98-99	-0.4319636667507538
2216	100	-0.5889607032585893
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	2.0
5	65.0
6	366.0
7	920.0
8	1222.0
9	1706.0
10	2666.0
11	4276.0
12	5463.0
13	6284.0
14	6803.0
15	7150.0
16	7507.0
17	7959.0
18	10048.0
19	10903.0
20	11529.0
21	12402.0
22	13480.0
23	17120.0
24	30250.0
25	67315.0
26	33363.0
27	114912.0
28	46378.0
29	103396.0
30	60670.0
31	73001.0
32	88839.0
33	214193.0
34	195216.0
35	976114.0
36	137058.0
37	133837.0
38	19297.0
39	2929.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.500811863392487	47.24323196279575	19.59273616316289	19.663220010648878
2	9.05167578411123	60.24454603044721	15.637764697342632	15.066013488098932
3	6.94622360981082	66.66362845253168	11.397592249666964	14.99255568799053
4	6.118963532603966	66.89274656766167	10.892088330771461	16.096201568962893
5	6.0450537394933725	66.35128224163388	11.387947176098917	16.215716842773833
6	5.975630277054648	64.32591452450005	12.030076118265415	17.668379080179886
7	8.359529597310287	67.9508678792698	9.688589592047665	14.00101293137225
8	6.860650183388126	66.42724958230896	8.64083271777778	18.07126751652514
9	5.8083243080055	64.9081231249933	8.973595451399744	20.309957115601456
10-11	9.198989715271772	73.02274584138084	8.29302876317245	9.485235680174936
12-13	9.073795072024918	73.50672077213713	7.726217465882662	9.69326668995528
14-15	10.554699172338559	72.434223754203	7.2350209982873475	9.776056075171084
16-17	9.841980599998598	73.05939152178944	7.976713234423764	9.121914643788209
18-19	10.421077660366667	72.16122073277539	8.634518234421558	8.783183372436381
20-21	10.275724782111164	72.0021480066494	8.395249721239633	9.326877489999788
22-23	10.721004880251838	72.44397196613417	8.883481280682018	7.951541872931976
24-25	10.570517410341608	71.96170731224449	8.800178673362655	8.66759660405125
26-27	12.123593767722909	71.7543591911651	8.355045937780345	7.767001103331649
28-29	11.574454078766426	72.70798050184084	8.92653708040722	6.791028338985512
30-31	12.288613512525266	72.78290507971018	8.348218217035319	6.580263190729238
32-33	13.033557570174292	72.9237675171498	7.556470128942887	6.486204783733022
34-35	12.726624764579178	73.68393264757782	7.264944220836485	6.3244983670065205
36-37	13.214373585606864	73.56413768241327	6.918830203940719	6.302658528039141
38-39	13.624569980587387	73.52035272264338	6.953894164646108	5.901183132123123
40-41	13.736789506094677	73.45500688185118	7.321798825568893	5.486404786485257
42-43	13.752648404481944	73.48988873504737	7.665329111559024	5.092133748911664
44-45	13.837941395431328	72.86733029360028	7.738244750067539	5.556483560900866
46-47	13.206198129886692	72.30521852806686	8.431698752590993	6.056884589455458
48-49	13.172180715964387	72.20581357077278	8.68249321312879	5.939512500134046
50-51	13.981304780174025	71.80963937214615	8.762270697115413	5.446785150564408
52-53	14.425397116071295	71.592190712226	8.905975048405402	5.076437123297304
54-55	14.462308082067285	71.66266286640752	8.782520952671431	5.092508098853756
56-57	14.304127352191909	71.59790977226218	8.368128873317424	5.729834002228482
58-59	13.575844816848079	71.42840349929264	8.524555679751206	6.47119600410808
60-61	13.610380718458895	71.12994664868354	9.070259992287054	6.18941264057051
62-63	13.426082222178684	70.48615830074027	9.565461281704128	6.522298195376923
64-65	12.897836470791313	70.5794303554035	9.790461019579148	6.732272154226035
66-67	12.09299917961553	70.64359966062727	10.533237532445195	6.730163627311997
68-69	11.730160039597425	71.22407606005609	9.801600395974262	7.2441635043722155
70-71	11.336587474498824	71.30044241910775	9.939683995779571	7.4232861106138515
72-73	11.292327770614358	72.16427551421945	9.640774408357855	6.902622306808329
74-75	11.042787377337998	73.11764950955825	9.175276570871814	6.664286542231947
76-77	10.251971092635115	73.98180409297564	9.142668805905718	6.62355600848353
78-79	9.564852595128876	74.74010760967063	8.888574469897486	6.806465325302999
80-81	9.196554040661033	75.8130236834935	8.219475606453408	6.770946669392061
82-83	8.691738696311385	76.79811135786167	8.14957497920427	6.360574966622673
84-85	8.050535715795794	77.90233960959686	8.249633433731274	5.797491240876063
86-87	7.527615591950084	78.74740014536911	8.055589244006411	5.669395018674402
88-89	7.222559453203169	79.42253680613179	7.786384902447431	5.568518838217614
90-91	7.170837149056589	80.14345056885215	7.492100421585855	5.193611860505408
92-93	7.22192280585382	80.81645171596517	6.900986960228087	5.060638517952916
94-95	7.38359751474496	80.9449892707669	6.484214703463137	5.187198511024999
96-97	7.592408375397533	80.64293305981033	6.446326095327827	5.31833246946431
98-99	8.030229842337372	80.16103689959728	6.219198944653286	5.589534313412069
100	8.852319442828822	79.67961997256054	5.809063931630701	5.658996652979937
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	787121.0
1	669893.0
2	331718.5
3	63060.5
4	12646.5
5	8890.5
6	6544.5
7	4314.5
8	2889.5
9	2274.5
10	2026.0
11	1924.0
12	1953.0
13	1936.5
14	1978.0
15	2060.5
16	2082.5
17	2032.5
18	1955.5
19	1928.0
20	1895.0
21	1867.0
22	1828.0
23	1778.5
24	1776.5
25	1832.0
26	1879.0
27	1889.5
28	1916.5
29	2023.0
30	2181.5
31	2329.5
32	2634.5
33	3182.5
34	3829.5
35	4603.0
36	5669.5
37	7082.5
38	8997.0
39	11351.0
40	14495.5
41	19039.5
42	25118.0
43	33248.5
44	43025.5
45	53250.5
46	62295.5
47	68267.0
48	69903.0
49	67367.5
50	62449.5
51	56432.0
52	50711.5
53	45447.5
54	39573.5
55	33299.5
56	26841.5
57	20277.5
58	14290.0
59	9456.5
60	5981.5
61	3856.0
62	2615.5
63	1841.5
64	1363.0
65	1055.5
66	867.0
67	719.5
68	609.0
69	520.0
70	444.0
71	374.5
72	297.5
73	270.0
74	225.0
75	198.5
76	203.0
77	175.0
78	170.0
79	157.5
80	153.0
81	155.5
82	133.5
83	118.5
84	104.5
85	105.0
86	111.5
87	101.0
88	88.5
89	75.0
90	74.0
91	76.0
92	67.0
93	61.0
94	50.5
95	48.5
96	42.5
97	38.5
98	32.5
99	27.0
100	38.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.649706991416987E-4
2	7.011254713522194E-4
3	1.23728024356274E-4
4	5.773974469959455E-4
5	5.361547722105207E-4
6	0.001031066869635617
7	0.0011135522192064662
8	0.0014022509427044389
9	6.186401217813701E-4
10-11	4.1242674785424676E-4
12-13	0.0010723095444210415
14-15	0.0029076085723724393
16-17	0.0014022509427044389
18-19	0.003979918116793481
20-21	0.004103646141149755
22-23	0.004351102189862303
24-25	0.0056502464456031805
26-27	0.008166049607514086
28-29	0.009856999273716497
30-31	0.00963016456239666
32-33	0.009382708513684113
34-35	0.01200161836255858
36-37	0.0110324155051011
38-39	0.00901152444061529
40-41	0.007423681461376441
42-43	0.004124267478542468
44-45	0.004866635624680112
46-47	0.006908148026558633
48-49	0.0057327317951740295
50-51	0.003959296779400768
52-53	0.00406240346636433
54-55	0.004186131490720604
56-57	0.006289507904777262
58-59	0.007547409485732716
60-61	0.007341196111805592
62-63	0.008475369668404769
64-65	0.009547679212825812
66-67	0.008248534957084936
68-69	0.011259250216420935
70-71	0.011341735565991785
72-73	0.013156413256550472
74-75	0.017734350157732608
76-77	0.018414854291692116
78-79	0.018497339641262968
80-81	0.02303403386765968
82-83	0.020518230705748774
84-85	0.020064561283109104
86-87	0.020580094717926913
88-89	0.021384326876242695
90-91	0.020332638669214365
92-93	0.021136870827530144
94-95	0.018765417027368225
96-97	0.020683201404890474
98-99	0.018394232954299407
100	0.017610622133376337
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2424673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.64284878157651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.86495469880407	36.839156907429164
2	1.698383383512837	1.2786397775743197
3	0.15092052596174008	0.1704323561044132
4	0.05812287168631469	0.08751641878555676
5	0.041043548717039635	0.07724980489073958
6	0.025913865944073747	0.058528304224728815
7	0.019511554255166753	0.05141293404245375
8	0.016438822451956242	0.04950432861649389
9	0.010671764296192777	0.03615440486707908
>10	0.08484804664465678	0.6201130253073837
>50	0.008861264744450583	0.23547356495659677
>100	0.010929925173880146	0.9457206748584147
>500	0.0026231798533040726	0.6886157087968218
>1k	0.0032789748166300913	2.6792117070646015
>5k	0.0010929916055433637	2.8576502760466065
>10k+	0.0024045815321954	53.32461980643465
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	568421	23.44320244420588	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	129590	5.344638225443184	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	103647	4.2746795134849105	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	98230	4.051267944172265	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44539	1.8369074922680295	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43493	1.7937676544424754	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32891	1.356512816367403	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31701	1.3074340333727477	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31029	1.2797189559169422	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30866	1.272996399926918	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23897	0.9855761993472933	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19273	0.7948700711394897	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17766	0.7327173602378547	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16645	0.6864843218033937	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16034	0.6612850475094992	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15831	0.652912784528058	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12853	0.5300920990170633	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12828	0.5290610321474277	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11985	0.49429345730331475	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11740	0.48418900198088566	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11740	0.48418900198088566	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11071	0.4565976525494365	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8584	0.35402712035808537	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8568	0.3533672375615186	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8488	0.35006782357868466	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8290	0.34190177397117055	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6654	0.2744287580222158	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6385	0.2633344785049366	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6276	0.2588390269533252	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5668	0.23376348068378708	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5309	0.21895736043581962	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5234	0.21586415982691276	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4826	0.1990371485144595	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4510	0.18600446328226528	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3775	0.15569109731497813	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3421	0.14109119044093782	No Hit
TAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3228	0.13313135420735084	No Hit
CAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3041	0.12541897402247643	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2818	0.11622185754532673	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2707	0.11164392064414458	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2683	0.11065409644929441	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2667	0.1099942136527276	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2436	0.1004671557772945	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.004454208876825865	0.0	0.0	0.0	0.0
2	0.010269426021570743	0.0	0.0	0.0	0.0
3	0.017074467361165815	0.0	0.0	0.0	0.0
4	0.027591349431449107	0.0	0.0	0.0	0.0
5	0.045201971564825444	0.0	0.0	0.0	0.0
6	0.07881475151494655	0.0	0.0	0.0	0.0
7	0.13259519943514034	0.0	0.0	0.0	0.0
8	0.31723865444948657	0.0	0.0	0.0	0.0
9	0.9446634659601522	0.0	0.0	0.0	0.0
10-11	2.1507023833729333	0.0	0.0	0.0	0.0
12-13	3.441206298746264	0.0	0.0	0.0	0.0
14-15	4.8407764675896505	0.0	0.0	0.0	0.0
16-17	6.102884801373216	0.0	0.0	0.0	0.0
18-19	7.267784150687536	0.0	0.0	0.0	0.0
20-21	8.67896413248302	0.0	0.0	0.0	0.0
22-23	10.25608401627766	0.0	0.0	0.0	0.0
24-25	11.743562946426177	0.0	0.0	0.0	0.0
26-27	13.25636487889295	0.0	0.0	0.0	0.0
28-29	14.90603062763515	0.0	0.0	0.0	0.0
30-31	16.74106157820044	0.0	0.0	0.0	0.0
32-33	18.814970101122913	0.0	0.0	0.0	0.0
34-35	20.731620305088562	0.0	0.0	0.0	0.0
36-37	22.077389404674364	0.0	0.0	0.0	0.0
38-39	23.047149038241447	0.0	0.0	0.0	0.0
40-41	23.95267320583023	0.0	0.0	0.0	0.0
42-43	24.632414350306206	4.1242674785424676E-5	0.0	0.0	0.0
44-45	25.124315732471967	4.1242674785424676E-5	0.0	0.0	0.0
46-47	25.502469817579524	4.1242674785424676E-5	0.0	0.0	0.0
48-49	25.816450300720962	4.1242674785424676E-5	0.0	0.0	0.0
50-51	26.133503363133915	4.1242674785424676E-5	0.0	0.0	0.0
52-53	26.48167402367247	4.1242674785424676E-5	0.0	0.0	0.0
54-55	26.839825411509096	4.1242674785424676E-5	0.0	0.0	0.0
56-57	27.229981114979218	4.1242674785424676E-5	0.0	0.0	0.0
58-59	27.71584044528891	4.1242674785424676E-5	0.0	0.0	0.0
60-61	28.231951277553716	4.1242674785424676E-5	0.0	0.0	0.0
62-63	28.645141014891493	4.1242674785424676E-5	0.0	0.0	0.0
64-65	29.000137338107038	4.1242674785424676E-5	0.0	0.0	0.0
66-67	29.29539364689589	4.1242674785424676E-5	0.0	0.0	0.0
68-69	29.520393059187775	4.1242674785424676E-5	0.0	0.0	0.0
70-71	29.709346373717196	4.1242674785424676E-5	0.0	0.0	0.0
72-73	29.88056533808889	4.1242674785424676E-5	0.0	0.0	0.0
74-75	30.036318299416045	4.1242674785424676E-5	0.0	0.0	0.0
76-77	30.187905750589874	4.1242674785424676E-5	0.0	0.0	0.0
78-79	30.323841606682635	4.1242674785424676E-5	0.0	0.0	0.0
80-81	30.446868505567558	4.1242674785424676E-5	0.0	0.0	0.0
82-83	30.56362651788509	4.1242674785424676E-5	0.0	0.0	0.0
84-85	30.67605404935016	4.1242674785424676E-5	0.0	0.0	0.0
86-87	30.78012993917118	4.1242674785424676E-5	0.0	0.0	0.0
88	30.86110993111236	4.1242674785424676E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAAAAA	2465	0.0	71.87105	1
CTAAAAA	3535	0.0	64.73946	1
AGTTGCC	30	1.3723943E-4	62.658104	3
ACTAGCC	45	2.2062704E-7	62.658096	3
CGAAAAA	1895	0.0	59.763683	1
GCAAAAA	1215	0.0	56.468483	1
GTCGCTT	25	0.005239145	56.393456	7
GTTACGG	25	0.00524	56.39113	1
ATGCGAC	25	0.00524	56.39113	1
TTGCAAC	25	0.00524	56.39113	1
TCAAAAA	1625	0.0	55.81276	1
TAAAACA	1040	0.0	54.67409	1
TCGCTTT	35	2.9404464E-4	53.705833	1
GAAAACA	760	0.0	51.939198	1
GTTTGCC	55	8.8234447E-7	51.26572	3
CAGGCCT	85	1.4370016E-10	49.7579	4
TACTTTC	85	1.4370016E-10	49.756878	1
ATACGCC	95	7.2759576E-12	49.466923	3
CTAACGA	210	0.0	49.231365	5
TACCCGA	1130	0.0	47.82532	6
>>END_MODULE