##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248472_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2424673
Sequences flagged as poor quality	0
Sequence length	100
%GC	20
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.472394009419006	34.0	31.0	34.0	31.0	34.0
2	32.69556678364464	34.0	31.0	34.0	31.0	34.0
3	32.83570898013876	34.0	31.0	34.0	31.0	34.0
4	36.304216692312735	37.0	37.0	37.0	35.0	37.0
5	36.23856041618808	37.0	37.0	37.0	35.0	37.0
6	36.204008128106345	37.0	37.0	37.0	35.0	37.0
7	36.17955575865282	37.0	37.0	37.0	35.0	37.0
8	36.17679332429569	37.0	37.0	37.0	35.0	37.0
9	37.9077228145816	39.0	39.0	39.0	35.0	39.0
10-11	37.99101631436486	39.0	39.0	39.0	35.0	39.0
12-13	37.97794919149922	39.0	39.0	39.0	35.0	39.0
14-15	39.54588453783252	41.0	40.0	41.0	36.0	41.0
16-17	39.361493281774486	41.0	39.5	41.0	36.0	41.0
18-19	38.552791242365466	40.0	37.5	41.0	35.0	41.0
20-21	37.644787565168585	39.0	35.0	41.0	34.0	41.0
22-23	36.59539286328507	36.5	35.0	40.5	33.0	41.0
24-25	35.733677695920235	35.0	35.0	39.5	33.0	41.0
26-27	35.48847143511723	35.0	35.0	39.0	33.0	41.0
28-29	35.31581186411528	35.0	35.0	39.0	33.0	41.0
30-31	35.24806747136624	35.0	35.0	39.0	33.0	41.0
32-33	35.192498741067354	35.0	35.0	39.0	33.0	41.0
34-35	35.10926421830902	35.0	35.0	39.0	32.5	41.0
36-37	35.01482632915861	35.0	35.0	39.0	31.5	41.0
38-39	34.92250913834567	35.0	35.0	39.0	31.0	41.0
40-41	34.80463056255421	35.0	35.0	38.5	31.0	41.0
42-43	34.71571465513081	35.0	35.0	38.0	31.0	41.0
44-45	34.60029269926295	35.0	35.0	38.0	31.0	41.0
46-47	34.44534025825338	35.0	35.0	37.0	30.5	41.0
48-49	34.230237850629756	35.0	35.0	36.5	29.5	40.0
50-51	34.00018023048881	35.0	35.0	35.5	28.0	39.5
52-53	33.75179312839298	35.0	35.0	35.0	27.5	39.0
54-55	33.48685472226565	35.0	35.0	35.0	27.0	38.0
56-57	33.22896819488649	35.0	34.5	35.0	27.0	37.0
58-59	33.04178192275825	35.0	34.0	35.0	26.0	36.5
60-61	32.86479372682419	35.0	34.0	35.0	26.0	35.5
62-63	32.69694985674357	35.0	33.5	35.0	26.0	35.0
64-65	32.56557399698846	35.0	33.0	35.0	26.0	35.0
66-67	32.43790482262969	35.0	33.0	35.0	26.0	35.0
68-69	32.315705664227714	35.0	33.0	35.0	25.0	35.0
70-71	32.25537299256436	35.0	33.0	35.0	25.0	35.0
72-73	32.18121288932569	35.0	33.0	35.0	24.0	35.0
74-75	32.12649788239486	35.0	33.0	35.0	24.0	35.0
76-77	32.06494319027762	35.0	33.0	35.0	24.0	35.0
78-79	31.97479103367753	35.0	33.0	35.0	24.0	35.0
80-81	31.89506255070271	35.0	33.0	35.0	24.0	35.0
82-83	31.808453964720194	35.0	33.0	35.0	24.0	35.0
84-85	31.663232320399494	35.0	33.0	35.0	23.0	35.0
86-87	31.533564319807248	35.0	33.0	35.0	20.0	35.0
88-89	31.344119598807758	35.0	33.0	35.0	20.0	35.0
90-91	31.178123400557517	35.0	33.0	35.0	20.0	35.0
92-93	30.916347482732725	35.0	32.0	35.0	18.5	35.0
94-95	30.48297378656833	35.0	31.0	35.0	15.0	35.0
96-97	30.135847390555345	35.0	31.0	35.0	11.5	35.0
98-99	29.784999049356344	35.0	29.5	35.0	10.0	35.0
100	29.303568769891857	35.0	29.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.270325408364279
1101	2	0.21663271938442108
1101	3	0.16932558700462153
1101	4	0.09288129308426818
1101	5	0.0775387540178869
1101	6	0.130570170782093
1101	7	0.1582535127019824
1101	8	0.2295123448430445
1101	9	0.27661555525988746
1101	10-11	0.2421932354867451
1101	12-13	0.22867513554661656
1101	14-15	0.3096259106999
1101	16-17	0.37147991613853293
1101	18-19	0.36127424437657396
1101	20-21	0.2914746892168978
1101	22-23	0.2509259461083957
1101	24-25	0.28914720133393956
1101	26-27	0.3056552989023018
1101	28-29	0.3743288809062548
1101	30-31	0.37808647544365215
1101	32-33	0.3298239219426975
1101	34-35	0.4420469821694937
1101	36-37	0.49496770362733855
1101	38-39	0.509308375017099
1101	40-41	0.45850893496881895
1101	42-43	0.4089219791425336
1101	44-45	0.4560959417414523
1101	46-47	0.3933482504964516
1101	48-49	0.4567774105404112
1101	50-51	0.44037195611203117
1101	52-53	0.49817729930091303
1101	54-55	0.5237121473808131
1101	56-57	0.5092363129615478
1101	58-59	0.49823751634935576
1101	60-61	0.5416964192625571
1101	62-63	0.5477180847158252
1101	64-65	0.5255787825165399
1101	66-67	0.5823753367993234
1101	68-69	0.6442447540408409
1101	70-71	0.6761842210032327
1101	72-73	0.7136893762247141
1101	74-75	0.670812624605496
1101	76-77	0.6518924087087541
1101	78-79	0.6218977789440814
1101	80-81	0.6964734467020044
1101	82-83	0.6929464948123147
1101	84-85	0.5497197622328329
1101	86-87	0.5350936600948266
1101	88-89	0.6046358692719238
1101	90-91	0.6402712168517084
1101	92-93	0.6069471293783018
1101	94-95	0.6549600278735497
1101	96-97	0.7121550979610447
1101	98-99	0.683853801131395
1101	100	0.6386628766749283
1102	1	0.2651027580318015
1102	2	0.23191674804432694
1102	3	0.22269973670960752
1102	4	0.10056687556430433
1102	5	0.14009930093234146
1102	6	0.20231269276391117
1102	7	0.1739899063988588
1102	8	0.18641052974466987
1102	9	0.28997384153509387
1102	10-11	0.19172875478542295
1102	12-13	0.234555780524083
1102	14-15	0.3023428436548983
1102	16-17	0.31771877874749777
1102	18-19	0.3466877081309363
1102	20-21	0.26455128101972036
1102	22-23	0.30160394592125783
1102	24-25	0.3781514545301192
1102	26-27	0.34989551408373387
1102	28-29	0.4364722389141278
1102	30-31	0.4469956103731647
1102	32-33	0.4925004596345701
1102	34-35	0.5946651063792245
1102	36-37	0.6175758649519025
1102	38-39	0.5173070250577183
1102	40-41	0.6105849447574272
1102	42-43	0.5823914581709815
1102	44-45	0.6045404683305335
1102	46-47	0.6070670117057091
1102	48-49	0.5730150741458431
1102	50-51	0.5945616493249162
1102	52-53	0.630807793448156
1102	54-55	0.6414417814314675
1102	56-57	0.6610226221904938
1102	58-59	0.7011789644742663
1102	60-61	0.6467929983436775
1102	62-63	0.6297548510083288
1102	64-65	0.6759942276920796
1102	66-67	0.6460170974591151
1102	68-69	0.6237295825255487
1102	70-71	0.6678422519298906
1102	72-73	0.6348171551264485
1102	74-75	0.6508584118668992
1102	76-77	0.6233622553780123
1102	78-79	0.6205801816024277
1102	80-81	0.634824363577895
1102	82-83	0.6959763656787281
1102	84-85	0.26551466891006115
1102	86-87	0.3218839979957302
1102	88-89	0.47566238614455614
1102	90-91	0.5333935195334618
1102	92-93	0.5135406337138626
1102	94-95	0.7162564451645679
1102	96-97	0.6879515584181988
1102	98-99	0.710360774196225
1102	100	0.8062348261833101
1103	1	0.2176924289201665
1103	2	0.18634433079427737
1103	3	0.19400632999882816
1103	4	0.12155110390387591
1103	5	0.12887540381332485
1103	6	0.12562531541857425
1103	7	0.13904609633852516
1103	8	0.13535396761962915
1103	9	0.21143201910621912
1103	10-11	0.1634961646673858
1103	12-13	0.1829974914758239
1103	14-15	0.26080913556227614
1103	16-17	0.2944266629243941
1103	18-19	0.2242021317231675
1103	20-21	0.1555887998790979
1103	22-23	0.1644063852854316
1103	24-25	0.19460582429356066
1103	26-27	0.23357429312031996
1103	28-29	0.2007400102254806
1103	30-31	0.14063749684008542
1103	32-33	0.17028933471133456
1103	34-35	0.26501717852269024
1103	36-37	0.23774331455118158
1103	38-39	0.21382127702383258
1103	40-41	0.25522579010107194
1103	42-43	0.22287255426346775
1103	44-45	0.21972723784511317
1103	46-47	0.17435140087783907
1103	48-49	0.1599461446071473
1103	50-51	0.20051311135491545
1103	52-53	0.21406999165461826
1103	54-55	0.22455006097781904
1103	56-57	0.2668943771816643
1103	58-59	0.2850769871854695
1103	60-61	0.33536815682071364
1103	62-63	0.2858126845945037
1103	64-65	0.24515199060811455
1103	66-67	0.30380518908661003
1103	68-69	0.2771723488792759
1103	70-71	0.2860453950644697
1103	72-73	0.27367536518159596
1103	74-75	0.20741527633384038
1103	76-77	0.17913308726116384
1103	78-79	0.20270144658555012
1103	80-81	0.24209435005916546
1103	82-83	0.2609147282303077
1103	84-85	0.14745529904939048
1103	86-87	0.13205292525170975
1103	88-89	0.16775061944109382
1103	90-91	0.1943315187299106
1103	92-93	0.24660847732048197
1103	94-95	0.2267859939306689
1103	96-97	0.23112806756553184
1103	98-99	0.1800229225024239
1103	100	0.22853248749330746
1104	1	0.2701124703913891
1104	2	0.23446024542188582
1104	3	0.18197595341590755
1104	4	0.152094127392445
1104	5	0.15633075820635156
1104	6	0.1388442785995636
1104	7	0.20359471869554113
1104	8	0.16905394491206494
1104	9	0.22459419154266413
1104	10-11	0.20830511055515188
1104	12-13	0.236756309221839
1104	14-15	0.26211807485357497
1104	16-17	0.28396427879825836
1104	18-19	0.3348300215529676
1104	20-21	0.3315313318394004
1104	22-23	0.27597293875213325
1104	24-25	0.25789434739957784
1104	26-27	0.2791849495802623
1104	28-29	0.32749136033805826
1104	30-31	0.3231232399605233
1104	32-33	0.31635381911646476
1104	34-35	0.34714974437133606
1104	36-37	0.36944175010595615
1104	38-39	0.4488729701437748
1104	40-41	0.422640711231395
1104	42-43	0.44545710628977986
1104	44-45	0.42526779530481207
1104	46-47	0.45592602824100226
1104	48-49	0.5056062002778106
1104	50-51	0.44765297656164904
1104	52-53	0.43413984951262563
1104	54-55	0.44816165177655876
1104	56-57	0.4526866521900601
1104	58-59	0.4778301037523178
1104	60-61	0.45228985907173325
1104	62-63	0.4947444358367079
1104	64-65	0.44826033153466227
1104	66-67	0.456105035131273
1104	68-69	0.5129081083598095
1104	70-71	0.42426205385179117
1104	72-73	0.49983052605798406
1104	74-75	0.49514973364628645
1104	76-77	0.41007675634691765
1104	78-79	0.5077027802943448
1104	80-81	0.49109135998277864
1104	82-83	0.4779947718141493
1104	84-85	0.478665080827021
1104	86-87	0.47837887424470793
1104	88-89	0.510305095089091
1104	90-91	0.6668767322443259
1104	92-93	0.6115476365221504
1104	94-95	0.5552865950825421
1104	96-97	0.6265987610512909
1104	98-99	0.7129539531137681
1104	100	0.7054635191530991
1105	1	0.22437378711628497
1105	2	0.19646128127173057
1105	3	0.17267911122262092
1105	4	0.13654943811554432
1105	5	0.1500835644496803
1105	6	0.12467173920435926
1105	7	0.12875897733525932
1105	8	0.1545607036608132
1105	9	0.18269584561944896
1105	10-11	0.1830342207941129
1105	12-13	0.20111193532218152
1105	14-15	0.2749542340164268
1105	16-17	0.23491126801460638
1105	18-19	0.2923408230887432
1105	20-21	0.2715881369825226
1105	22-23	0.22268726779599035
1105	24-25	0.2300248089472845
1105	26-27	0.2993707100258618
1105	28-29	0.29967433303765034
1105	30-31	0.35392882962972294
1105	32-33	0.385182995194441
1105	34-35	0.390127753876925
1105	36-37	0.4468197498769655
1105	38-39	0.4258113462245632
1105	40-41	0.40845771860532665
1105	42-43	0.3361562461358787
1105	44-45	0.3908871598496404
1105	46-47	0.37836346361267914
1105	48-49	0.3818919272567314
1105	50-51	0.39399152012278904
1105	52-53	0.3605489817817684
1105	54-55	0.368170630121881
1105	56-57	0.34216023238406024
1105	58-59	0.42489547352147383
1105	60-61	0.35734357152850293
1105	62-63	0.42958577294213285
1105	64-65	0.3938057362045484
1105	66-67	0.43330339449669
1105	68-69	0.42113000725779415
1105	70-71	0.3839260576123351
1105	72-73	0.3917037760605595
1105	74-75	0.44498549542303323
1105	76-77	0.5102365519470524
1105	78-79	0.4767021317040374
1105	80-81	0.4626053667404655
1105	82-83	0.4814407446987907
1105	84-85	0.5473212069348037
1105	86-87	0.5151651819496088
1105	88-89	0.40209814921118436
1105	90-91	0.5252299008554289
1105	92-93	0.683460270705293
1105	94-95	0.6482559970058368
1105	96-97	0.591454835578908
1105	98-99	0.5680843824851252
1105	100	0.5164034459053504
1106	1	0.19127779587228133
1106	2	0.19501590002095526
1106	3	0.15434363299033294
1106	4	0.09140769437284035
1106	5	0.07694026515408581
1106	6	0.07145138391287276
1106	7	0.05336092819101168
1106	8	0.07699634666943922
1106	9	0.14709902247538764
1106	10-11	0.10190688596134834
1106	12-13	0.09305352992425497
1106	14-15	0.09100272877847004
1106	16-17	0.10391391004902317
1106	18-19	0.17404006415533502
1106	20-21	0.16758896286884806
1106	22-23	0.19404761482861232
1106	24-25	0.1783915185119298
1106	26-27	0.16738317048554308
1106	28-29	0.16834734219055747
1106	30-31	0.20253512641157556
1106	32-33	0.17938643585290492
1106	34-35	0.21706812568613287
1106	36-37	0.21529182008531933
1106	38-39	0.29589189018697226
1106	40-41	0.28272919465663193
1106	42-43	0.26414104341285594
1106	44-45	0.27679814052951457
1106	46-47	0.2759899625362152
1106	48-49	0.26892803773306184
1106	50-51	0.29728484207011974
1106	52-53	0.24210216926197603
1106	54-55	0.2661960000668273
1106	56-57	0.311812678571151
1106	58-59	0.21497629812486707
1106	60-61	0.21572956513790587
1106	62-63	0.19954684402849665
1106	64-65	0.2103999173141986
1106	66-67	0.17837814522736295
1106	68-69	0.1363510521242759
1106	70-71	0.06701286788700855
1106	72-73	0.17408361160493513
1106	74-75	0.22312877467283698
1106	76-77	0.18697233647892375
1106	78-79	0.2287489666751057
1106	80-81	0.22284178672370913
1106	82-83	0.2123588817237554
1106	84-85	0.21423086860205132
1106	86-87	0.2576608019456792
1106	88-89	0.28575313720375917
1106	90-91	0.2752533544233806
1106	92-93	0.28640375362027015
1106	94-95	0.4222470726225467
1106	96-97	0.3405760684748458
1106	98-99	0.36147893564454137
1106	100	0.17012502599427393
1107	1	0.13330404910709603
1107	2	0.07072818392747848
1107	3	0.11386970082094905
1107	4	0.06572191350889511
1107	5	0.03886245784174491
1107	6	0.05712100533700948
1107	7	0.11330027174304291
1107	8	0.14001386834221563
1107	9	0.12401850240684809
1107	10-11	0.13868787871302146
1107	12-13	0.12209251164534862
1107	14-15	0.13523088133294436
1107	16-17	0.16424334403927077
1107	18-19	0.13635625052754108
1107	20-21	0.10673102932551615
1107	22-23	0.16391876866725852
1107	24-25	0.13276401311568975
1107	26-27	0.2212640121815852
1107	28-29	0.17864968765127998
1107	30-31	0.1834370885735055
1107	32-33	0.19859173490011983
1107	34-35	0.23040501097362664
1107	36-37	0.327118031945723
1107	38-39	0.34486100379875495
1107	40-41	0.2772192550398529
1107	42-43	0.29268739641464947
1107	44-45	0.30765029726827464
1107	46-47	0.21746199382463516
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2216	62-63	-0.8065699034653662
2216	64-65	-0.808927704993124
2216	66-67	-0.7999333590411979
2216	68-69	-0.8478614425022357
2216	70-71	-0.8401363101927224
2216	72-73	-0.8371689729857508
2216	74-75	-0.8259649636300992
2216	76-77	-0.9285814319973227
2216	78-79	-0.8782988382412142
2216	80-81	-0.8985650151834541
2216	82-83	-0.8334781987552908
2216	84-85	-0.7991447008251669
2216	86-87	-0.8847930758802072
2216	88-89	-1.0159253225518157
2216	90-91	-1.14343660747587
2216	92-93	-1.1816184889790158
2216	94-95	-1.2909829588093444
2216	96-97	-1.1874206428633585
2216	98-99	-1.2283833557289299
2216	100	-1.0757788084350075
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	65.0
7	208.0
8	498.0
9	836.0
10	1223.0
11	1862.0
12	2591.0
13	3627.0
14	4800.0
15	6210.0
16	7724.0
17	9560.0
18	11394.0
19	13254.0
20	15094.0
21	17099.0
22	19136.0
23	21274.0
24	23280.0
25	25791.0
26	28515.0
27	31823.0
28	36813.0
29	42994.0
30	48033.0
31	57694.0
32	73731.0
33	110681.0
34	177495.0
35	1082003.0
36	195607.0
37	308452.0
38	41214.0
39	4027.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.109952691125542	20.671345282153034	40.67147733762953	23.547224689091887
2	10.610537656228992	18.557046730729727	52.480649506740804	18.351766106300467
3	7.46244957567474	11.815820112650242	63.72892344658434	16.992806865090675
4	7.644082315429751	10.136212182013821	67.94371859628082	14.275986906275609
5	7.546828195776646	10.36029366579493	68.89161000238796	13.201268136040461
6	7.339381434115033	7.327297330402905	64.34999688617805	20.98332434930401
7	9.614451103303415	7.349485889437463	62.395753984145486	20.64030902311363
8	9.159544400420181	6.332936441326314	63.04384137572365	21.46367778252985
9	8.169761448244774	7.732424124820131	68.37020909623689	15.727605330698202
10-11	9.390214680495061	11.058336526203739	70.33822705164778	9.21322174165341
12-13	8.968054661391452	13.903091262203194	67.49108024051078	9.637773835894572
14-15	10.942588959418446	10.944589229145539	67.7677773456462	10.345044465789819
16-17	12.690721908846115	12.858456143910777	69.44541708579604	5.005404861447068
18-19	13.284244927047894	9.768080066879122	67.89548528811926	9.05218971795372
20-21	11.25435759218608	9.587001821914319	69.12976009914843	10.028880486751167
22-23	11.732613016270648	10.346962250167342	70.74345695275198	7.176967780810031
24-25	11.394611024787883	10.57955249626496	69.78994070333209	8.235895775615058
26-27	13.765629427143372	9.509096690564048	68.31572752284535	8.409546359447233
28-29	12.05801772032765	11.755090274028705	69.51714726068217	6.669744744961485
30-31	12.177679766549241	11.161931408682971	69.26439956356867	7.395989261199122
32-33	12.914561641668948	11.757840137636103	68.53302192126755	6.794576299427403
34-35	12.624635825829888	12.376189030727556	68.55865390008677	6.44052124335578
36-37	12.750317259275787	11.048995060364842	68.75716849241115	7.4435191879482305
38-39	13.033485849185	10.997432223654723	67.82315584909426	8.14592607806601
40-41	13.184038820944949	11.841856450185706	67.64943937743293	7.324665351436412
42-43	13.063466666446704	11.80213856214865	67.45645225848585	7.677942512918786
44-45	13.389676477156756	10.514064511552414	67.37279126840743	8.723467742883402
46-47	13.126206737127157	10.462832341823223	68.09972167329266	8.311239247756962
48-49	11.875899224571212	9.95847456456239	68.98489003709182	9.180736173774575
50-51	11.7454042667706	9.862465678283723	69.04146651035657	9.350663544589104
52-53	12.033728266394741	10.163599413941471	68.97943949131275	8.823232828351045
54-55	11.527767782151107	10.590774874039639	68.93669926008533	8.944758083723919
56-57	11.024877189523858	10.233470850272058	68.98818437635457	9.75346758384952
58-59	10.253774426489676	10.565713397229235	69.92526414902133	9.255248027259759
60-61	9.73756651158724	10.046804278435244	70.09825867132929	10.117370538648226
62-63	9.634536285923916	9.52524319774254	70.24703949769722	10.593181018636328
64-65	9.89838629786367	9.66961318082892	70.22266507689903	10.20933544440838
66-67	9.178206710760586	9.754016314777292	70.74855042308798	10.319226551374145
68-69	9.33786095603859	8.774176465930237	71.08557363635164	10.802388941679535
70-71	9.03527387450887	8.573164321405866	71.43944785239384	10.952113951691416
72-73	8.878707323365271	7.9665732302501375	72.32457060182249	10.830148844562098
74-75	8.448648184945885	7.456325943449531	72.86871676346351	11.22630910814108
76-77	8.452918261298489	7.511471141554639	73.06736190861318	10.968248688533684
78-79	8.418309532986108	7.629948163641796	73.11130949029952	10.840432813072571
80-81	8.13308950623776	7.235111266733727	73.52098368890763	11.110815538120873
82-83	8.737651055275801	7.566575058962107	73.29877027637592	10.397003609386179
84-85	8.50549436046502	8.700675721377111	72.67904306898923	10.11478684916863
86-87	8.475670953928246	9.410501704669533	72.06711364277972	10.04671369862251
88-89	8.452885174348168	10.540530564158761	71.42866864455031	9.577915616942766
90-91	8.374919885740775	11.639416133052771	70.84455482867303	9.141109152533424
92-93	8.150830608904233	12.07017293508758	70.36984872561882	9.40914773038937
94-95	8.159843305272197	12.67007535716392	69.93916902136323	9.230912316200655
96-97	7.665092078067206	13.60521674325146	70.12786770238812	8.60182347629322
98-99	7.411925119596406	14.235941983318575	69.94272900458753	8.409403892497483
100	7.386829968828182	14.712330891444699	69.45999094293306	8.440848196794063
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240584.0
1	468955.5
2	533583.0
3	222385.0
4	47052.0
5	15195.5
6	9769.0
7	7665.0
8	6507.5
9	5831.5
10	5460.5
11	5168.0
12	5200.5
13	5556.5
14	5838.5
15	5760.0
16	5469.5
17	5380.0
18	5375.0
19	5037.0
20	4370.0
21	3783.0
22	3486.5
23	3298.0
24	3230.5
25	3207.5
26	3096.0
27	2933.0
28	2794.5
29	2672.0
30	2554.0
31	2454.0
32	2369.5
33	2360.0
34	2480.0
35	2533.5
36	2537.0
37	2599.5
38	2650.0
39	2763.0
40	3135.0
41	3921.5
42	5378.5
43	8698.5
44	15046.5
45	23448.5
46	32652.0
47	41251.5
48	48412.0
49	55897.0
50	66373.0
51	79007.0
52	89495.5
53	93995.5
54	89091.5
55	75298.0
56	56628.5
57	37575.5
58	22220.0
59	12171.5
60	6409.0
61	3333.5
62	1790.0
63	990.0
64	618.0
65	432.5
66	376.0
67	330.5
68	276.0
69	216.5
70	147.0
71	109.0
72	76.5
73	54.5
74	44.0
75	31.0
76	21.5
77	12.5
78	9.5
79	8.0
80	7.5
81	5.0
82	5.0
83	4.0
84	4.0
85	4.0
86	1.5
87	0.5
88	0.0
89	1.0
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059760635764080354
2	0.007712380184874414
3	0.0
4	0.0
5	4.94912097425096E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.649706991416987E-4
18-19	0.0
20-21	0.0010516882070283292
22-23	0.0
24-25	2.0621337392712338E-5
26-27	0.0
28-29	0.0
30-31	3.0932006089068504E-4
32-33	7.629894835303565E-4
34-35	3.7118407306882203E-4
36-37	0.0
38-39	2.8869872349797273E-4
40-41	1.0310668696356169E-4
42-43	2.6807738610526037E-4
44-45	2.6807738610526037E-4
46-47	1.8559203653441102E-4
48-49	5.980187843886577E-4
50-51	2.268347113198357E-4
52-53	2.0621337392712338E-5
54-55	6.1864012178137E-5
56-57	8.248534957084935E-5
58-59	0.0
60-61	6.1864012178137E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	4.1242674785424676E-5
70-71	0.0012991442557408774
72-73	0.001216658906170028
74-75	2.8869872349797273E-4
76-77	6.1864012178137E-5
78-79	9.692028574574798E-4
80-81	1.649706991416987E-4
82-83	9.279601826720551E-4
84-85	2.0621337392712338E-4
86-87	3.918054104615344E-4
88-89	0.0011341735565991785
90-91	4.536694226396714E-4
92-93	2.47456048712548E-4
94-95	0.003546870031546522
96-97	7.629894835303565E-4
98-99	0.0024745604871254804
100	0.0019384057149149595
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2424673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.235335807976824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.98057804011462	38.050655462556
2	2.240387215416451	1.7580468947352514
3	0.26328673798467983	0.30990430735847163
4	0.12250422890503118	0.1922597823594464
5	0.06258613060957294	0.12277939256942459
6	0.04258527843397006	0.10025086198998054
7	0.034214997086186166	0.0939705830241824
8	0.028386850660633442	0.08910140945606736
9	0.018676986957619022	0.0659518069647047
>10	0.13568073317671817	1.1047053785767778
>50	0.02557541177840579	0.7246105631198927
>100	0.029206770298487873	2.1436396570325758
>500	0.0033498704774710653	0.8527145618696146
>1k	0.008479359646098633	7.601814162390548
>5k	8.374676193677663E-4	2.0251406091972073
>10k+	0.003663920834733978	44.76445456679986
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62200	2.5652943716534145	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55860	2.3038158135138223	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55321	2.281586011804478	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52669	2.1722104382735323	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47011	1.9388593843375994	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45920	1.893863626146701	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44888	1.851301185768143	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43392	1.7896021442891474	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41798	1.7238613206811804	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38705	1.596297727569862	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37602	1.5508070572815387	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36682	1.512863796478948	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36008	1.4850662336735716	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34566	1.4255942966329893	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33933	1.3994876834938155	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32409	1.3366338471208283	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32321	1.3330044917397108	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28622	1.1804478377084249	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28364	1.1698072276137854	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25709	1.060307926058483	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24275	1.001165930416184	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23799	0.9815344172183218	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22886	0.9438798551392291	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22167	0.9142263719685086	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21513	0.887253662658841	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21415	0.8832118805298694	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20162	0.8315348090237323	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19496	0.8040671876166394	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18863	0.7779605744774656	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18782	0.7746199178198463	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13388	0.5521569300272655	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13224	0.5453931313624559	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12763	0.5263802582863751	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12248	0.5051402807718813	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10917	0.4502462806324811	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8858	0.36532761324929175	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7085	0.2922043508547338	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6265	0.2583853575306856	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5689	0.23462957685428096	No Hit
TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5572	0.22980418390438628	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5438	0.22427766548313938	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5234	0.21586415982691276	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5165	0.2130184152667184	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4853	0.20015070073366595	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4779	0.1970987427995445	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4746	0.1957377345316255	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4670	0.1926032912479332	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4669	0.19256204857314782	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4208	0.17354917549706703	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4021	0.16583679531219261	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3999	0.1649294564669133	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3947	0.16278483737807117	No Hit
TTTTTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3935	0.1622899252806461	No Hit
TTTTTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3871	0.1596503940943789	No Hit
TTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3830	0.1579594444281765	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3627	0.1495871814467353	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3617	0.14917475469888106	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3529	0.14554539931776367	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3489	0.1438956923263467	No Hit
CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3341	0.13779177645810384	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3321	0.13696692296239532	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3106	0.12809974788352904	No Hit
TCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2895	0.11939754350380444	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2890	0.1191913301298773	No Hit
TTTTTTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2698	0.11127273657107577	No Hit
ATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2673	0.11024166970144014	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2626	0.10830326398652519	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2620	0.10805580793781264	No Hit
GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2577	0.10628237292203938	No Hit
TTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2554	0.1053337914019746	No Hit
TTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2551	0.10521006337761835	No Hit
AACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2536	0.10459142325583697	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.004949120974250961	0.0	0.0	0.0	0.0
2	0.012084103712129429	0.0	0.0	0.0	0.0
3	0.019920211921360117	0.0	0.0	0.0	0.0
4	0.03221052900741667	0.0	0.0	0.0	0.0
5	0.05464654409068769	0.0	0.0	0.0	0.0
6	0.09856999273716498	0.0	0.0	0.0	0.0
7	0.17136331373343952	0.0	0.0	0.0	0.0
8	0.4513185901769022	0.0	0.0	4.1242674785424676E-5	0.0
9	1.3714014219649413	0.0	0.0	4.1242674785424676E-5	0.0
10-11	3.0563502789860735	0.0	0.0	4.1242674785424676E-5	0.0
12-13	4.640130854758559	0.0	0.0	4.1242674785424676E-5	0.0
14-15	6.289446040765085	0.0	0.0	4.1242674785424676E-5	0.0
16-17	7.60294274733129	0.0	0.0	4.1242674785424676E-5	0.0
18-19	8.75757267062404	0.0	0.0	4.1242674785424676E-5	0.0
20-21	10.15732843150396	0.0	0.0	4.1242674785424676E-5	0.0
22-23	11.758678386735035	0.0	0.0	4.1242674785424676E-5	0.0
24-25	13.338520287065514	0.0	0.0	4.1242674785424676E-5	0.0
26-27	15.007219530221189	0.0	0.0	4.1242674785424676E-5	0.0
28-29	16.865573213377637	0.0	0.0	4.1242674785424676E-5	0.0
30-31	18.885082648258134	0.0	0.0	4.1242674785424676E-5	0.0
32-33	21.299181374148183	0.0	0.0	4.1242674785424676E-5	0.0
34-35	23.776752576533	0.0	0.0	4.1242674785424676E-5	0.0
36-37	25.272170721577716	0.0	0.0	4.1242674785424676E-5	0.0
38-39	26.285853803791277	0.0	0.0	4.1242674785424676E-5	0.0
40-41	27.322344085161173	0.0	0.0	4.1242674785424676E-5	0.0
42-43	28.085766616776777	0.0	0.0	4.1242674785424676E-5	0.0
44-45	28.622560650446474	0.0	0.0	4.1242674785424676E-5	0.0
46-47	29.040761372770678	0.0	0.0	4.1242674785424676E-5	0.0
48-49	29.374249641085623	0.0	0.0	4.1242674785424676E-5	0.0
50-51	29.691632644896856	0.0	0.0	4.1242674785424676E-5	0.0
52-53	30.023863011630848	0.0	0.0	4.1242674785424676E-5	0.0
54-55	30.29697200405993	0.0	0.0	4.1242674785424676E-5	0.0
56-57	30.561667490832782	0.0	0.0	4.1242674785424676E-5	0.0
58-59	30.830940914506822	0.0	0.0	4.1242674785424676E-5	0.0
60-61	31.093636131552586	0.0	0.0	4.1242674785424676E-5	0.0
62-63	31.32129569636813	0.0	0.0	4.1242674785424676E-5	0.0
64-65	31.529798038745845	0.0	0.0	4.1242674785424676E-5	0.0
66-67	31.71359601892709	0.0	0.0	4.1242674785424676E-5	0.0
68-69	31.867204361165403	0.0	0.0	4.1242674785424676E-5	0.0
70-71	32.00903791975247	0.0	0.0	4.1242674785424676E-5	0.0
72-73	32.14089075104148	0.0	0.0	4.1242674785424676E-5	0.0
74-75	32.26428883399947	0.0	0.0	4.1242674785424676E-5	0.0
76-77	32.384201910938096	0.0	0.0	4.1242674785424676E-5	0.0
78-79	32.4846278240406	0.0	0.0	4.1242674785424676E-5	0.0
80-81	32.577155764921706	0.0	0.0	6.186401217813701E-5	0.0
82-83	32.66892071631928	0.0	0.0	8.248534957084935E-5	0.0
84-85	32.758458563278424	0.0	0.0	8.248534957084935E-5	0.0
86-87	32.845789927136565	0.0	0.0	8.248534957084935E-5	0.0
88	32.91429400995516	0.0	0.0	1.2372802435627403E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCTTCC	25	5.5404475E-5	75.25517	1
AACCGGC	25	5.561446E-5	75.197754	3
GCATTTT	225	0.0	73.16476	1
CTTAGGC	85	0.0	71.9351	1
ACTTTTC	755	0.0	71.01896	1
CATTTTT	5245	0.0	70.753494	1
CCGTACG	20	0.0021628186	70.503716	2
CTCCCTA	2140	0.0	68.96056	6
TGTTCGC	75	0.0	68.936966	2
GATTTTT	4265	0.0	68.373695	1
CATTTTC	395	0.0	67.87254	1
CCCCTAC	4345	0.0	66.95542	7
AACTTTT	380	0.0	66.83848	1
CACTTTT	595	0.0	65.61114	1
CCCCCTA	2505	0.0	65.47908	6
ATTTTTC	4530	0.0	65.20454	1
TCCCCTA	1545	0.0	65.09838	6
AATTTTT	3925	0.0	64.94953	1
GCCTTTT	470	0.0	64.04696	1
GTTACAC	200	0.0	63.448105	7
>>END_MODULE