##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248471_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3190123
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.16125428392573	31.0	31.0	34.0	23.0	34.0
2	30.52858338064081	33.0	31.0	34.0	26.0	34.0
3	30.845549528968004	34.0	31.0	34.0	26.0	34.0
4	34.318390231348445	37.0	35.0	37.0	30.0	37.0
5	34.24620962890773	37.0	35.0	37.0	30.0	37.0
6	34.148186135769684	37.0	35.0	37.0	29.0	37.0
7	34.25560926647656	37.0	35.0	37.0	30.0	37.0
8	34.17467727733382	37.0	35.0	37.0	30.0	37.0
9	35.640617618819086	39.0	35.0	39.0	28.0	39.0
10-11	35.99074048242027	39.0	37.0	39.0	30.0	39.0
12-13	35.99749461070937	39.0	37.0	39.0	30.0	39.0
14-15	37.14012578825331	40.0	37.0	41.0	30.5	41.0
16-17	36.92137732620341	40.0	37.0	41.0	30.0	41.0
18-19	36.375811998471534	39.0	35.5	41.0	30.0	41.0
20-21	36.0388704448073	39.0	35.0	41.0	28.5	41.0
22-23	35.827851622022095	38.0	35.0	41.0	27.5	41.0
24-25	35.634268960789285	38.0	35.0	40.5	26.5	41.0
26-27	35.46153518218577	38.0	35.0	40.0	25.0	41.0
28-29	35.299009160461836	38.0	35.0	40.0	25.0	41.0
30-31	35.23466477624844	38.0	35.0	40.0	25.0	41.0
32-33	35.15373090630048	38.0	34.5	40.0	25.0	41.0
34-35	35.037145432950396	38.0	34.0	40.0	25.0	41.0
36-37	34.94020261914666	38.0	34.0	40.0	25.0	41.0
38-39	34.75709259486233	38.0	33.0	40.0	25.0	41.0
40-41	34.53395198241573	37.0	33.0	40.0	25.0	41.0
42-43	34.2548121185296	37.0	33.0	40.0	25.0	41.0
44-45	34.01622993846946	36.0	33.0	39.0	25.0	41.0
46-47	33.80453371233648	35.5	33.0	39.0	25.0	41.0
48-49	33.64202508806087	35.0	33.0	39.0	25.0	40.0
50-51	33.321021948056554	35.0	32.0	38.5	25.0	40.0
52-53	32.9156057619095	35.0	32.0	37.0	25.0	39.5
54-55	32.713334877683394	35.0	32.0	36.5	25.0	39.0
56-57	32.571765414687775	35.0	32.0	36.0	25.0	39.0
58-59	32.28196357946073	35.0	31.5	35.5	25.0	37.0
60-61	32.02069810474392	35.0	31.0	35.0	25.0	37.0
62-63	31.804485281601995	35.0	31.0	35.0	25.0	36.0
64-65	31.69412809474744	35.0	31.0	35.0	25.0	36.0
66-67	31.612934046743653	35.0	31.0	35.0	24.0	35.5
68-69	31.506434391401207	35.0	31.0	35.0	24.0	35.0
70-71	31.396634549827702	35.0	31.0	35.0	24.0	35.0
72-73	31.286684557303904	35.0	30.5	35.0	23.5	35.0
74-75	31.16869772732901	35.0	30.0	35.0	23.0	35.0
76-77	31.008512994640018	34.0	30.0	35.0	22.5	35.0
78-79	30.865020565037774	34.5	30.0	35.0	20.0	35.0
80-81	30.675941805378663	34.0	30.0	35.0	20.0	35.0
82-83	30.506803969627505	34.0	29.5	35.0	19.5	35.0
84-85	30.338090568921636	34.0	29.0	35.0	17.5	35.0
86-87	30.092431389009136	34.0	29.0	35.0	15.5	35.0
88-89	29.802268282445535	34.0	28.5	35.0	12.5	35.0
90-91	29.36629794525164	34.0	27.5	35.0	10.0	35.0
92-93	28.818059366362988	34.0	26.0	35.0	8.0	35.0
94-95	28.09397067135029	33.5	25.0	35.0	7.5	35.0
96-97	27.15379218920399	33.0	22.0	35.0	7.0	35.0
98-99	25.934244541668143	32.5	17.0	35.0	7.0	35.0
100	24.256201720121762	29.0	10.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.486960372532824
1101	2	0.504529442580175
1101	3	0.555005752280465
1101	4	0.46890834375271595
1101	5	0.5599829268568683
1101	6	0.6410708974000201
1101	7	0.4795481444666905
1101	8	0.45900875532568364
1101	9	0.5134301240729116
1101	10-11	0.5475633002578064
1101	12-13	0.5459226375712163
1101	14-15	0.7208553387617798
1101	16-17	0.714511267011126
1101	18-19	0.7255461620735133
1101	20-21	0.7089007045108247
1101	22-23	0.6802047841787626
1101	24-25	0.6217180838872807
1101	26-27	0.6874465356531374
1101	28-29	0.7358600794233325
1101	30-31	0.6463161371939066
1101	32-33	0.6924883789213894
1101	34-35	0.7034080021075653
1101	36-37	0.7193193609830146
1101	38-39	0.6735831114544908
1101	40-41	0.6768729689762694
1101	42-43	0.7070070856317869
1101	44-45	0.6670639460566647
1101	46-47	0.7611024814350245
1101	48-49	0.7557434266721188
1101	50-51	0.7418922207487171
1101	52-53	0.7358734028422234
1101	54-55	0.681322307373982
1101	56-57	0.7053169520799685
1101	58-59	0.7810406258081386
1101	60-61	0.6209363037446494
1101	62-63	0.6131268523478575
1101	64-65	0.6036879415179683
1101	66-67	0.5439161285833265
1101	68-69	0.6189492840095134
1101	70-71	0.65021735500871
1101	72-73	0.6850373661534626
1101	74-75	0.6647847114716257
1101	76-77	0.638524130959329
1101	78-79	0.6781856971587565
1101	80-81	0.5790664374771453
1101	82-83	0.5963173947359195
1101	84-85	0.5335809470711617
1101	86-87	0.5841352630865941
1101	88-89	0.5838708118831555
1101	90-91	0.574309433388887
1101	92-93	0.4937325008013964
1101	94-95	0.5998866243442471
1101	96-97	0.7102867667416888
1101	98-99	0.5877643002730615
1101	100	0.48988614490792415
1102	1	0.3686929146287419
1102	2	0.28472516869987885
1102	3	0.2954222973109246
1102	4	0.199362428115478
1102	5	0.25697496377240725
1102	6	0.17578558731432992
1102	7	0.22843579725107332
1102	8	0.14872035856824084
1102	9	0.16700370286762478
1102	10-11	0.39097142052464307
1102	12-13	0.481575781424219
1102	14-15	0.6005357287338313
1102	16-17	0.607021668008251
1102	18-19	0.5996282223138394
1102	20-21	0.5694445226209837
1102	22-23	0.6070222640801504
1102	24-25	0.5440265266834103
1102	26-27	0.5717225422576249
1102	28-29	0.5629753012672154
1102	30-31	0.5479174179130482
1102	32-33	0.5847358107576355
1102	34-35	0.6361784999868405
1102	36-37	0.6196991596415558
1102	38-39	0.570871891769805
1102	40-41	0.6591889243378901
1102	42-43	0.6510231715675516
1102	44-45	0.7478667567781869
1102	46-47	0.6338561861753362
1102	48-49	0.5944050328809425
1102	50-51	0.4928099108727224
1102	52-53	0.5022980501657486
1102	54-55	0.48283269133715123
1102	56-57	0.5142268018815273
1102	58-59	0.47499480209556566
1102	60-61	0.4010304428275546
1102	62-63	0.42856285427680874
1102	64-65	0.49542987578919195
1102	66-67	0.5057182573589145
1102	68-69	0.5320419117686477
1102	70-71	0.5862655987189136
1102	72-73	0.5762472240020955
1102	74-75	0.5210164990804174
1102	76-77	0.4953842368870909
1102	78-79	0.5574732780480574
1102	80-81	0.5391435543436494
1102	82-83	0.6438197163477355
1102	84-85	0.6414301976648566
1102	86-87	0.7164957710746407
1102	88-89	0.6241915555507767
1102	90-91	0.6601292163996781
1102	92-93	0.6498646984588135
1102	94-95	0.6843981108160513
1102	96-97	0.7912411503735761
1102	98-99	0.7081694325476668
1102	100	0.47264389668312745
1103	1	0.348595945266716
1103	2	0.24651386100944706
1103	3	0.3234671301592975
1103	4	0.2676458416382701
1103	5	0.3924879435433226
1103	6	0.25480646328847456
1103	7	0.2962850664304142
1103	8	0.34037898537194167
1103	9	0.31721819615479063
1103	10-11	0.36672401582653436
1103	12-13	0.42802878030365576
1103	14-15	0.6105301861962431
1103	16-17	0.5803574053647438
1103	18-19	0.5259703528269739
1103	20-21	0.4810297087374664
1103	22-23	0.5009794669267293
1103	24-25	0.45566086848629084
1103	26-27	0.5073875743830385
1103	28-29	0.5432883112471387
1103	30-31	0.4983090643307406
1103	32-33	0.5850211394255993
1103	34-35	0.633129443767757
1103	36-37	0.5500444510910185
1103	38-39	0.5915041241717276
1103	40-41	0.5553949035078816
1103	42-43	0.5463511740318268
1103	44-45	0.6320202625556917
1103	46-47	0.6784263571286857
1103	48-49	0.5905822562496112
1103	50-51	0.5190368365302263
1103	52-53	0.4860624443736512
1103	54-55	0.40399371342093815
1103	56-57	0.4219535968883861
1103	58-59	0.4999193772910786
1103	60-61	0.4646702403061269
1103	62-63	0.47850472891615325
1103	64-65	0.4493947497852204
1103	66-67	0.3916022579756486
1103	68-69	0.4679145482292135
1103	70-71	0.44709874962208573
1103	72-73	0.4659779460694047
1103	74-75	0.4499615415367799
1103	76-77	0.47441529078556144
1103	78-79	0.4960308443384669
1103	80-81	0.49319782233153475
1103	82-83	0.4684872452847735
1103	84-85	0.4686252811714482
1103	86-87	0.4571101312836561
1103	88-89	0.43548257268211543
1103	90-91	0.519076974253597
1103	92-93	0.4165440402834406
1103	94-95	0.4751464821967808
1103	96-97	0.39816021782029765
1103	98-99	0.35283681946715717
1103	100	0.30013936381875084
1104	1	0.21881122112848317
1104	2	0.23587259843773012
1104	3	0.1944014470679889
1104	4	0.17571351097315357
1104	5	0.12780233854768142
1104	6	0.1299265069522093
1104	7	0.08247427585899914
1104	8	0.12634724403054776
1104	9	0.1711353967815299
1104	10-11	0.3028564427579141
1104	12-13	0.3595561212649869
1104	14-15	0.42296282589940404
1104	16-17	0.4672783432879086
1104	18-19	0.3510051289256495
1104	20-21	0.34228483454599257
1104	22-23	0.3672910392824278
1104	24-25	0.3171001370724156
1104	26-27	0.44371907806097965
1104	28-29	0.42173258957581794
1104	30-31	0.3693606424554261
1104	32-33	0.3356774243877325
1104	34-35	0.3967114772931666
1104	36-37	0.32651000166327293
1104	38-39	0.4185657303152084
1104	40-41	0.4177202371175781
1104	42-43	0.365112834929775
1104	44-45	0.3907335908582539
1104	46-47	0.40822574791528865
1104	48-49	0.33606850910704367
1104	50-51	0.2945840893816509
1104	52-53	0.2120835410081554
1104	54-55	0.2281082640058898
1104	56-57	0.3198366078253443
1104	58-59	0.3262356472925063
1104	60-61	0.31201328159323083
1104	62-63	0.337222262377626
1104	64-65	0.33873272873957916
1104	66-67	0.3241329251765457
1104	68-69	0.3136707332185722
1104	70-71	0.35022116750414867
1104	72-73	0.38451044637001175
1104	74-75	0.4223684506025833
1104	76-77	0.37797498350462533
1104	78-79	0.41699832699616834
1104	80-81	0.2964714665700541
1104	82-83	0.32143916145179574
1104	84-85	0.25389732385103514
1104	86-87	0.2954203318733377
1104	88-89	0.3240538555491206
1104	90-91	0.17956072072322726
1104	92-93	0.1633160281880066
1104	94-95	0.12008851654882235
1104	96-97	0.09861926900586937
1104	98-99	0.1621295472103128
1104	100	0.15326539824028984
1105	1	0.12357045986312087
1105	2	0.04901105367025238
1105	3	0.11987898232174388
1105	4	0.0023802029775197298
1105	5	0.07263354024951241
1105	6	0.12762140965983093
1105	7	0.18264122206871747
1105	8	0.16274670223254617
1105	9	0.028369149444060326
1105	10-11	0.22677417455816595
1105	12-13	0.243495663952622
1105	14-15	0.3114261226324331
1105	16-17	0.33886658538651915
1105	18-19	0.3668736190491586
1105	20-21	0.23649667077985725
1105	22-23	0.3487534904184315
1105	24-25	0.3287349654567535
1105	26-27	0.23896283112404149
1105	28-29	0.18762373745062888
1105	30-31	0.2818645442176475
1105	32-33	0.2358965126612631
1105	34-35	0.2688388033306026
1105	36-37	0.18884384360221418
1105	38-39	0.24282151576491628
1105	40-41	0.2517874135982652
1105	42-43	0.26199326700383097
1105	44-45	0.2373351815395921
1105	46-47	0.24012219000515245
1105	48-49	0.18574502463530962
1105	50-51	0.22193612720031552
1105	52-53	0.1781853767342909
1105	54-55	0.21656908544049003
1105	56-57	0.198264518510733
1105	58-59	0.17870459667158656
1105	60-61	0.13284129035500314
1105	62-63	0.21975614741014837
1105	64-65	0.22243392650140592
1105	66-67	0.11574157094565152
1105	68-69	0.1676369701480951
1105	70-71	0.17269040144509162
1105	72-73	0.20117030596601637
1105	74-75	0.2686688880969612
1105	76-77	0.25408378807289367
1105	78-79	0.31578780861508093
1105	80-81	0.3775657999005624
1105	82-83	0.3082162683327425
1105	84-85	0.3369299668977028
1105	86-87	0.2354950868602117
1105	88-89	0.2825923877107961
1105	90-91	0.26321211528589217
1105	92-93	0.22929393819609345
1105	94-95	0.18261784371885526
1105	96-97	0.1685510784324542
1105	98-99	0.11222455871411441
1105	100	0.12143851788325577
1106	1	0.14251719785709227
1106	2	0.08652847907035621
1106	3	0.1542799746184791
1106	4	0.17262844572329783
1106	5	0.1573864304050261
1106	6	0.12721318337162302
1106	7	0.17127952128447532
1106	8	0.16836538973031878
1106	9	0.17071649669959044
1106	10-11	0.24389111166989608
1106	12-13	0.2163214457127225
1106	14-15	0.2953039548041687
1106	16-17	0.3268629072057294
1106	18-19	0.3262341961517805
1106	20-21	0.32686303326897814
1106	22-23	0.259899758358479
1106	24-25	0.27723391548589404
1106	26-27	0.3008229665674449
1106	28-29	0.2823316309834212
1106	30-31	0.3266693699580614
1106	32-33	0.2843791699587612
1106	34-35	0.2904056258344383
1106	36-37	0.2731325375826188
1106	38-39	0.24032105408319637
1106	40-41	0.17788981781689017
1106	42-43	0.30114904554076816
1106	44-45	0.3513429309010334
1106	46-47	0.4003251418439433
1106	48-49	0.3405278610085034
1106	50-51	0.3215641556158815
1106	52-53	0.35728303133527106
1106	54-55	0.23777664366617302
1106	56-57	0.23077492066787642
1106	58-59	0.26836570133461635
1106	60-61	0.2090411250156734
1106	62-63	0.24627190460961756
1106	64-65	0.23336325758116772
1106	66-67	0.15107600778289054
1106	68-69	0.1984722103534331
1106	70-71	0.22051669308060795
1106	72-73	0.23120597303254442
1106	74-75	0.19861116647367183
1106	76-77	0.12287205026564507
1106	78-79	0.18611950330037175
1106	80-81	0.24731865792693597
1106	82-83	0.25489244863847915
1106	84-85	0.3135661012615678
1106	86-87	0.4363459362381654
1106	88-89	0.3387213603970274
1106	90-91	0.2701178773624697
1106	92-93	0.220740184241766
1106	94-95	0.2922802437914207
1106	96-97	0.2655319453920306
1106	98-99	0.345392593073111
1106	100	0.5442377442136497
1107	1	0.14802823948304322
1107	2	0.1777790864180524
1107	3	0.2127566659137301
1107	4	0.17840806501714468
1107	5	0.18462263532010326
1107	6	0.13509924584013078
1107	7	0.13997233871441495
1107	8	0.11964664684592208
1107	9	0.09039675839248673
1107	10-11	0.16741727378825288
1107	12-13	0.27044130253106147
1107	14-15	0.29487950616830005
1107	16-17	0.3192567401389468
1107	18-19	0.1441587299507887
1107	20-21	0.16331117877424361
1107	22-23	0.18802434935126655
1107	24-25	0.27572955333992155
1107	26-27	0.26715819918833716
1107	28-29	0.21142219616191937
1107	30-31	0.16541004011133253
1107	32-33	0.18977586885593922
1107	34-35	0.2051751340217578
1107	36-37	0.22307429047323524
1107	38-39	0.26940594918909255
1107	40-41	0.2353173663912571
1107	42-43	0.21928641996994713
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2216	62-63	-0.629970994110888
2216	64-65	-0.6058214751179634
2216	66-67	-0.6236903714268323
2216	68-69	-0.5324681508937559
2216	70-71	-0.646140743047436
2216	72-73	-0.697968871251863
2216	74-75	-0.8158077144929621
2216	76-77	-0.6971221411025645
2216	78-79	-0.6689220395671391
2216	80-81	-0.7641771732572238
2216	82-83	-0.7423246866300701
2216	84-85	-0.7163887673363512
2216	86-87	-0.5733464671999968
2216	88-89	-0.5289127345445941
2216	90-91	-0.48034987855530886
2216	92-93	-0.6457949747809799
2216	94-95	-0.6555738831134441
2216	96-97	-0.6811279054929749
2216	98-99	-0.696925046504969
2216	100	-0.7079613946957295
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	11.0
5	106.0
6	945.0
7	2255.0
8	4509.0
9	7660.0
10	11133.0
11	13733.0
12	15024.0
13	16568.0
14	16733.0
15	17172.0
16	16955.0
17	17150.0
18	17781.0
19	18974.0
20	20628.0
21	22526.0
22	23676.0
23	28709.0
24	47139.0
25	55155.0
26	45570.0
27	80439.0
28	58181.0
29	93864.0
30	91900.0
31	121157.0
32	156890.0
33	245433.0
34	283520.0
35	656445.0
36	546806.0
37	408406.0
38	24434.0
39	2505.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.256979208919546	28.793480366556974	24.731969162269234	29.21757126225425
2	14.984718395290445	31.203876982298418	25.72854854534797	28.082856077063173
3	13.32588951955114	33.32237241157937	21.987003000826615	31.364735068042876
4	12.393502521395105	31.380781599567914	22.06298299833328	34.1627328807037
5	11.990219742327826	31.994921789285602	22.84994200808752	33.16491646029905
6	11.771744190238252	29.24866109422731	23.52906879240652	35.45052592312793
7	16.62623432534937	31.985957820170697	19.805003161339933	31.582804693140005
8	13.512557004625572	32.464988470551944	17.880487459295843	36.141967065526636
9	10.611288882647381	30.533285351736865	19.239261819020776	39.61616394659498
10-11	18.060094100100198	40.52435751894094	17.80806544970892	23.607482931249937
12-13	19.168599730415973	42.25339331055453	14.596219554245948	23.98178740478355
14-15	22.10366245502416	40.00315355954115	13.930363386609473	23.96282059882522
16-17	21.2268638609943	38.933351305686216	16.06684461385825	23.772940219461237
18-19	21.55202261810028	35.79521578527225	19.120495333637514	23.53226626298996
20-21	19.432844860312308	35.70087106337317	20.979329525402594	23.886954550911927
22-23	20.503921709078636	37.956918175065546	21.07832618782542	20.460833928030397
24-25	21.515819376673313	38.97240862711795	18.864990503812816	20.64678149239592
26-27	24.653697451936775	39.98474253059566	16.734641352287664	18.626918665179897
28-29	24.02167384153919	41.797603387760375	17.868708610724816	16.31201415997562
30-31	24.672501574929907	41.57956025480399	17.476478721635	16.27145944863111
32-33	25.692466489385463	41.77724797188581	16.453999760176625	16.07628577855211
34-35	26.34734584401654	41.55343917865195	15.924685099805458	16.174529877526055
36-37	27.586399320458217	41.447361924185444	14.943476801733278	16.02276195362306
38-39	28.494282098800554	42.126049481002326	14.329997597066068	15.049670823131054
40-41	29.068385718228967	42.01309226610188	14.249262163043747	14.669259852625405
42-43	29.474136116364946	41.135364170233935	15.066402068841098	14.324097644560021
44-45	29.63240859588425	39.63985851270945	15.986208014279764	14.74152487712653
46-47	29.78739412262418	38.5728768958708	17.196047014743126	14.443681966761895
48-49	29.63560500259409	39.09670856394076	18.03962137278229	13.228065060682864
50-51	30.401949835619767	39.67097307612431	17.89721827108828	12.029858817167645
52-53	30.899379028407832	40.720520150307166	17.178521260651642	11.201579560633366
54-55	30.533453061669757	42.249520728765006	16.53798064858631	10.679045560978928
56-57	29.799178884417	43.14421153953764	15.918115406034964	11.138494170010393
58-59	29.2528861838891	42.52195722399056	16.72252272840913	11.502633863711209
60-61	28.986909771580503	40.96174219963425	18.23974963466706	11.811598394118182
62-63	28.826760894757225	39.422969460775334	19.302154945603217	12.448114698864226
64-65	27.865974192575532	38.92191671720507	19.735919143988916	13.476189946230482
66-67	26.558225694908156	37.97293035463154	20.591887508048977	14.876956442411327
68-69	26.42875594714098	36.80795907413956	20.288622871632043	16.47466210708741
70-71	26.841491125328865	35.83331870314683	20.43807794461764	16.88711222690667
72-73	27.697551504556785	35.97353476125528	20.24997072602744	16.0789430081605
74-75	27.473089919212935	36.785870767339986	19.73063661652126	16.010402696925826
76-77	25.756886472359113	38.126059330669186	19.439497678956734	16.67755651801497
78-79	24.09112549460572	39.799948926732434	18.77585309835868	17.333072480303166
80-81	22.36718236210301	42.48780132204274	18.317981615563514	16.82703470029073
82-83	20.365706832547406	45.36172494230962	18.835689023778468	15.436879201364503
84-85	18.48103631205745	48.326928913447524	19.20628356363326	13.985751210861764
86-87	16.96412558865764	51.24196572460542	18.938832905883753	12.855075780853184
88-89	16.060898550856415	54.26475939080756	17.892288462958415	11.782053595377615
90-91	15.731758897041786	57.27858549051735	16.090989098778195	10.898666513662663
92-93	15.702585966340235	59.45556062087272	14.367150282525554	10.474703130261494
94-95	15.994832462619065	60.22725313189071	13.212789858809787	10.565124546680432
96-97	16.492151726648355	59.89253038014748	12.781961959385283	10.833355933818881
98-99	17.40146998285971	58.459565438629326	12.587562990643539	11.55140158786742
100	18.82281236067714	56.990206092802275	12.158798119098275	12.028183427422313
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	292712.0
1	248952.0
2	126818.5
3	32283.5
4	15275.5
5	13078.0
6	9735.0
7	6047.5
8	3477.5
9	2239.5
10	1802.5
11	1685.5
12	1650.5
13	1704.0
14	1779.0
15	1817.5
16	1859.0
17	1930.0
18	1986.5
19	1997.5
20	2046.0
21	2185.0
22	2248.0
23	2292.0
24	2369.0
25	2435.0
26	2485.5
27	2506.5
28	2595.5
29	2680.0
30	2724.0
31	2863.5
32	3141.5
33	3496.5
34	4023.0
35	4826.0
36	5954.5
37	7645.0
38	10072.5
39	13557.5
40	18914.0
41	26906.0
42	38847.5
43	56377.5
44	80418.0
45	110687.5
46	144059.0
47	173090.5
48	191327.5
49	196907.5
50	192004.5
51	182560.5
52	174422.0
53	169325.0
54	162623.0
55	148109.0
56	124885.0
57	96159.0
58	67185.0
59	43795.5
60	26348.0
61	14498.0
62	8373.0
63	5336.5
64	3726.5
65	2858.0
66	2379.0
67	1988.0
68	1712.5
69	1506.0
70	1341.5
71	1218.0
72	1079.5
73	1000.0
74	939.0
75	864.5
76	789.0
77	729.0
78	724.5
79	710.0
80	658.0
81	624.5
82	628.5
83	589.5
84	544.0
85	542.5
86	535.0
87	501.0
88	495.0
89	451.5
90	409.5
91	404.5
92	374.5
93	341.0
94	311.5
95	293.0
96	268.0
97	229.5
98	183.0
99	162.5
100	157.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5673376857255975E-4
2	4.075077982886553E-4
3	6.269350742902389E-5
4	7.523220891482868E-4
5	7.209753354337748E-4
6	5.64241566861215E-4
7	6.26935074290239E-4
8	9.717493651498704E-4
9	3.4481429085963145E-4
10-11	2.9779416028786354E-4
12-13	7.209753354337748E-4
14-15	0.00241370003601742
16-17	0.0011911766411514541
18-19	0.0034951630391680823
20-21	0.0035735299234543622
22-23	0.0038713240837422256
24-25	0.004968460463750143
26-27	0.006582818280047509
28-29	0.00899651831606493
30-31	0.009357005983781818
32-33	0.008636030648348041
34-35	0.010736263147220341
36-37	0.01023471508778815
38-39	0.008541990387204506
40-41	0.007037346208907932
42-43	0.003902670837456737
44-45	0.004843073448892096
46-47	0.0072097533543377485
48-49	0.005579722161183127
50-51	0.00346381628545357
52-53	0.0038243039531704577
54-55	0.0038713240837422256
56-57	0.006096943597472574
58-59	0.007131386470051468
60-61	0.007037346208907932
62-63	0.00860468389463353
64-65	0.009545086506068888
66-67	0.00868305077891981
68-69	0.01112809756865174
70-71	0.011629645628083933
72-73	0.013463430720382882
74-75	0.01772658922555651
76-77	0.019090173012137775
78-79	0.01841621780727577
80-81	0.023212271125596096
82-83	0.02140983278701166
84-85	0.020657510697863375
86-87	0.02078289771272142
88-89	0.021550893178726965
90-91	0.020814244466435934
92-93	0.02122175226472459
94-95	0.01868266521384912
96-97	0.02079857108957868
98-99	0.017616875587555713
100	0.01846323793784754
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3190123.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.59938381263045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.81325192714479	79.64287217850088
2	1.0048377153136299	1.6197860137193987
3	0.06262498068633371	0.15142604563789136
4	0.02461211993201406	0.07934886803371573
5	0.013450689860925964	0.05420586573227143
6	0.008987459323964789	0.04346302101315852
7	0.008175725453083682	0.04612709036178485
8	0.005684359731722742	0.036652471339694584
9	0.005410609098947057	0.039248258348353
>10	0.0418354166504006	0.6590877244588411
>50	0.004395748584707294	0.24486780404860692
>100	0.004351331978800145	0.7156294199057549
>500	7.809561446148718E-4	0.44366490059101726
>1k	0.00101524298781437	2.0460137038684483
>5k	2.3428684334177768E-4	1.3478323778168
>10k+	3.514302650126665E-4	12.829774256623358
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	212015	6.6459819887822515	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49703	1.5580276998723874	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41561	1.302802431128831	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37913	1.1884494735782913	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17590	0.5513893978382651	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13926	0.4365348922282934	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12178	0.38174076673532653	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11743	0.3681049288695138	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11024	0.3455666129487797	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9662	0.3028723343896144	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8527	0.26729376892364337	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6916	0.21679414868956462	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6563	0.20572874462834193	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5610	0.17585528833841202	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5548	0.1739117896081123	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4797	0.15037037756851382	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4691	0.14704762167477556	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4257	0.13344313056267737	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4110	0.12883515776664412	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3712	0.11635914978826835	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3593	0.11262888609624143	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3454	0.10827168732992426	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3228	0.10118732099044457	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0027585143268770516	0.0	0.0	0.0	0.0
2	0.006833592309763605	0.0	0.0	0.0	0.0
3	0.011504258613225885	0.0	0.0	0.0	0.0
4	0.019246906780710338	0.0	0.0	0.0	0.0
5	0.031816955020229624	0.0	0.0	0.0	0.0
6	0.05633011642497797	0.0	0.0	0.0	0.0
7	0.08636030648348042	0.0	0.0	0.0	0.0
8	0.19604259773055774	0.0	0.0	0.0	0.0
9	0.591419202331697	0.0	0.0	0.0	0.0
10-11	1.4682662706108824	0.0	0.0	0.0	0.0
12-13	2.6684551034552584	0.0	0.0	0.0	0.0
14-15	4.122427254372324	0.0	0.0	0.0	0.0
16-17	5.682163352322151	0.0	0.0	0.0	0.0
18-19	7.370311426863479	0.0	0.0	0.0	0.0
20-21	9.565649976505608	0.0	0.0	0.0	0.0
22-23	12.155816562558872	0.0	0.0	0.0	0.0
24-25	14.998826064073391	0.0	0.0	0.0	0.0
26-27	18.293636326875173	0.0	0.0	3.1346753714511947E-5	0.0
28-29	22.593689961170774	0.0	0.0	3.1346753714511947E-5	0.0
30-31	27.978639068148784	0.0	0.0	3.1346753714511947E-5	0.0
32-33	33.7513475185753	0.0	0.0	3.1346753714511947E-5	0.0
34-35	38.53511290943954	0.0	0.0	3.1346753714511947E-5	0.0
36-37	41.92408882039972	0.0	0.0	3.1346753714511947E-5	0.0
38-39	44.265456222220905	0.0	0.0	3.1346753714511947E-5	0.0
40-41	46.16458362263775	0.0	0.0	3.1346753714511947E-5	0.0
42-43	47.64259873365384	0.0	0.0	3.1346753714511947E-5	0.0
44-45	48.780501566867486	0.0	0.0	3.1346753714511947E-5	0.0
46-47	49.726327166695455	0.0	0.0	3.1346753714511947E-5	0.0
48-49	50.5808239995762	0.0	0.0	3.1346753714511947E-5	0.0
50-51	51.44676239756272	0.0	0.0	3.1346753714511947E-5	0.0
52-53	52.39371648052442	0.0	0.0	3.1346753714511947E-5	0.0
54-55	53.39200087269363	0.0	0.0	3.1346753714511947E-5	0.0
56-57	54.46188125034678	0.0	0.0	3.1346753714511947E-5	0.0
58-59	55.578499637788255	0.0	0.0	3.1346753714511947E-5	0.0
60-61	56.69185796284344	0.0	0.0	3.1346753714511947E-5	0.0
62-63	57.71779019178884	0.0	0.0	3.1346753714511947E-5	0.0
64-65	58.65450955966274	0.0	0.0	3.1346753714511947E-5	0.0
66-67	59.45883591322341	0.0	0.0	3.1346753714511947E-5	0.0
68-69	60.153197854753564	0.0	0.0	3.1346753714511947E-5	0.0
70-71	60.77488234779662	0.0	0.0	3.1346753714511947E-5	0.0
72-73	61.3459575069676	0.0	0.0	3.1346753714511947E-5	0.0
74-75	61.871297752469104	0.0	0.0	3.1346753714511947E-5	0.0
76-77	62.37309658593101	0.0	0.0	3.1346753714511947E-5	0.0
78-79	62.848140965097585	0.0	0.0	3.1346753714511947E-5	0.0
80-81	63.304910186848595	0.0	0.0	3.1346753714511947E-5	0.0
82-83	63.740379289450594	0.0	0.0	3.1346753714511947E-5	0.0
84-85	64.17493933619488	0.0	0.0	3.1346753714511947E-5	0.0
86-87	64.60783800499229	0.0	0.0	3.1346753714511947E-5	0.0
88	64.93182237800862	0.0	0.0	3.1346753714511947E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCTCA	355	0.0	56.921124	2
AGCTCAC	370	0.0	54.613506	3
TTGCTGA	2355	0.0	54.079433	9
GCTTGCT	515	0.0	52.923344	7
TGCTTGC	250	0.0	48.87197	6
CCAGCTC	440	0.0	45.92499	1
ACTGCTG	270	0.0	45.253246	8
CTTGCTG	1705	0.0	44.92658	8
TGAAAAA	1465	0.0	42.983376	1
GTTGCTG	505	0.0	41.875626	8
ACTTGCT	455	0.0	40.2791	7
GTGCTGA	650	0.0	39.76519	9
TTTGCTG	585	0.0	37.75567	8
TACCCGA	2805	0.0	37.359283	6
CTGCTGA	2270	0.0	37.26494	9
CCTACCC	2300	0.0	36.57283	4
TATTGAC	90	6.202081E-7	36.550125	3
TCGACAC	515	0.0	36.498856	5
CTACCCG	2845	0.0	36.17332	5
TGGGTCC	65	1.4152881E-4	36.148476	1
>>END_MODULE