##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248470_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2507335
Sequences flagged as poor quality	0
Sequence length	100
%GC	22
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.343250503024127	31.0	27.0	34.0	15.0	34.0
2	29.31791204605687	31.0	29.0	34.0	16.0	34.0
3	30.22338458961407	31.0	30.0	34.0	25.0	34.0
4	34.10388759379979	35.0	35.0	37.0	30.0	37.0
5	33.884554317632066	35.0	35.0	37.0	28.0	37.0
6	33.506763555727495	35.0	35.0	37.0	27.0	37.0
7	33.837776364147594	36.0	35.0	37.0	29.0	37.0
8	33.73851838705239	35.0	35.0	37.0	28.0	37.0
9	35.08532046974178	38.0	34.0	39.0	27.0	39.0
10-11	36.25476711328961	39.0	35.0	39.0	31.5	39.0
12-13	36.45418781295679	39.0	35.0	39.0	32.0	39.0
14-15	37.748492522937696	40.0	37.5	41.0	32.0	41.0
16-17	37.462529139504696	39.5	37.0	41.0	31.0	41.0
18-19	36.33134463484137	38.0	35.5	40.0	31.0	41.0
20-21	35.38739757551345	35.5	34.0	39.5	30.0	41.0
22-23	34.525849557398594	35.0	34.0	38.0	29.0	40.0
24-25	33.87094564547617	35.0	33.0	37.0	26.0	40.0
26-27	33.602996807367184	35.0	33.0	37.0	25.0	40.0
28-29	33.374887081303456	35.0	33.0	37.0	25.0	39.0
30-31	33.240500571323736	35.0	33.0	36.0	25.0	39.0
32-33	33.131076820608335	35.0	33.0	36.0	25.0	39.0
34-35	32.987801590134545	35.0	33.0	35.0	25.0	39.0
36-37	32.80492734317512	35.0	32.5	35.0	25.0	39.0
38-39	32.60932763272558	35.0	31.5	35.0	25.0	39.0
40-41	32.405583019421016	35.0	31.0	35.0	25.0	39.0
42-43	32.2183200489763	35.0	31.0	35.0	25.0	39.0
44-45	32.00898962444189	35.0	30.0	35.0	24.0	38.5
46-47	31.903270604047727	35.0	29.5	35.0	23.5	38.0
48-49	31.9108707053505	35.0	30.0	35.0	24.0	38.0
50-51	31.810630609790874	35.0	29.5	35.0	24.0	37.5
52-53	31.703466628910775	35.0	29.0	35.0	24.0	37.0
54-55	31.621263213730913	35.0	29.5	35.0	24.0	36.5
56-57	31.820150478496092	35.0	30.0	35.0	25.0	36.0
58-59	31.8646786328911	35.0	30.0	35.0	25.0	35.5
60-61	31.75029503436916	34.5	30.0	35.0	25.0	35.0
62-63	31.626719205850037	34.0	30.0	35.0	25.0	35.0
64-65	31.553233612580687	34.0	30.0	35.0	25.0	35.0
66-67	31.493462979617803	34.0	30.0	35.0	25.0	35.0
68-69	31.44913962434218	34.0	30.0	35.0	25.0	35.0
70-71	31.434888437324886	34.0	30.0	35.0	25.0	35.0
72-73	31.41562475696307	34.0	30.0	35.0	25.0	35.0
74-75	31.378862018836735	34.0	30.0	35.0	25.0	35.0
76-77	31.279077187531783	34.0	30.0	35.0	24.5	35.0
78-79	31.162054332588188	34.0	30.0	35.0	24.0	35.0
80-81	31.052976766168065	34.0	30.0	35.0	24.0	35.0
82-83	30.938439618160317	34.0	30.0	35.0	24.0	35.0
84-85	30.824995862140483	34.0	30.0	35.0	23.0	35.0
86-87	30.655048088907147	34.0	30.0	35.0	21.0	35.0
88-89	30.459881507656537	34.0	29.0	35.0	20.0	35.0
90-91	30.181384218702327	34.0	29.0	35.0	18.0	35.0
92-93	29.88387750340501	34.0	28.5	35.0	15.0	35.0
94-95	29.599550718192823	34.0	28.0	35.0	11.0	35.0
96-97	29.230340381321206	33.5	28.0	35.0	10.0	35.0
98-99	28.77223446408238	33.0	27.0	35.0	8.0	35.0
100	27.736485551392214	33.0	25.0	35.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5212982581032968
1101	2	0.3636806592589821
1101	3	0.3324345608772603
1101	4	0.280799437545177
1101	5	0.25679005362778184
1101	6	0.202257176731635
1101	7	0.19809746978332043
1101	8	0.22869883837135774
1101	9	0.28247219285748315
1101	10-11	0.38090921453996884
1101	12-13	0.43121243569883205
1101	14-15	0.630715013232475
1101	16-17	0.644119791863325
1101	18-19	0.6516922281746389
1101	20-21	0.6533494895139711
1101	22-23	0.5235720299992934
1101	24-25	0.5155230132411432
1101	26-27	0.5497369123486351
1101	28-29	0.5183601719791753
1101	30-31	0.5543614242857302
1101	32-33	0.50236955882648
1101	34-35	0.4438801373535952
1101	36-37	0.5392514448200103
1101	38-39	0.51031815894045
1101	40-41	0.560586797198404
1101	42-43	0.5932913672803934
1101	44-45	0.569276424663336
1101	46-47	0.5424187796858533
1101	48-49	0.6047569441743299
1101	50-51	0.5684125247659075
1101	52-53	0.5037017178296743
1101	54-55	0.499912703880355
1101	56-57	0.4717583991957781
1101	58-59	0.4647711933434664
1101	60-61	0.4969917877959631
1101	62-63	0.4280066849429005
1101	64-65	0.4808653940610341
1101	66-67	0.5069706135150476
1101	68-69	0.4332956183602228
1101	70-71	0.4636642880407784
1101	72-73	0.4839613530511322
1101	74-75	0.4823920096717238
1101	76-77	0.5211951966109218
1101	78-79	0.5515421566073577
1101	80-81	0.6019542303961884
1101	82-83	0.5934741563257795
1101	84-85	0.5434313252190677
1101	86-87	0.4274116237189105
1101	88-89	0.4351647997335881
1101	90-91	0.5074610752081306
1101	92-93	0.36362289214087085
1101	94-95	0.4556222672736929
1101	96-97	0.4835493635430055
1101	98-99	0.49077479789803036
1101	100	0.4918994379835411
1102	1	0.11373045356582523
1102	2	0.1692319401191007
1102	3	0.21051177781299302
1102	4	0.095977352574252
1102	5	0.18889116215202506
1102	6	0.24944761937459248
1102	7	0.0771109201901865
1102	8	0.14540797636725245
1102	9	0.13436436950333075
1102	10-11	0.16343112749073896
1102	12-13	0.27298180781171766
1102	14-15	0.42769446733232286
1102	16-17	0.4544968380417629
1102	18-19	0.3698837884524977
1102	20-21	0.37123692500641425
1102	22-23	0.3803093659509713
1102	24-25	0.2637017374054196
1102	26-27	0.2791788796747312
1102	28-29	0.36422641119634136
1102	30-31	0.27686160695003537
1102	32-33	0.3062791160933145
1102	34-35	0.3040793902791492
1102	36-37	0.22355778503024482
1102	38-39	0.261036864035475
1102	40-41	0.2761740155683938
1102	42-43	0.4331909334865074
1102	44-45	0.3272880809008427
1102	46-47	0.3052446550537766
1102	48-49	0.2579041373231057
1102	50-51	0.2708498528697767
1102	52-53	0.24615001481206278
1102	54-55	0.3636717908655456
1102	56-57	0.41752933726040453
1102	58-59	0.4457815479287035
1102	60-61	0.4313135980939009
1102	62-63	0.4323828705695263
1102	64-65	0.48695077631652595
1102	66-67	0.44573306062133256
1102	68-69	0.42556864781833426
1102	70-71	0.46601402748481036
1102	72-73	0.4510232812103929
1102	74-75	0.4656194578469304
1102	76-77	0.46544415767952074
1102	78-79	0.5322994422068277
1102	80-81	0.5496642063927482
1102	82-83	0.4348841082377142
1102	84-85	0.48561266170835893
1102	86-87	0.46042578313388205
1102	88-89	0.2883122183938518
1102	90-91	0.31582313118729743
1102	92-93	0.30357865580844035
1102	94-95	0.2982539050914248
1102	96-97	0.34937278263986116
1102	98-99	0.38460435271129967
1102	100	0.4070591316848251
1103	1	0.35549859976262255
1103	2	0.17275348691565995
1103	3	0.15406927735736886
1103	4	0.28995568151704276
1103	5	0.28558997090660654
1103	6	0.22791490568769746
1103	7	0.21385976456615197
1103	8	0.24473779726623235
1103	9	0.3052882060094575
1103	10-11	0.2947812608638287
1103	12-13	0.40909112404678893
1103	14-15	0.5746012055731242
1103	16-17	0.5309424035934001
1103	18-19	0.4877528840006562
1103	20-21	0.46007831871385463
1103	22-23	0.3512833209189026
1103	24-25	0.30754172282549774
1103	26-27	0.2558907772176795
1103	28-29	0.33163739915134727
1103	30-31	0.3179939528254039
1103	32-33	0.2656798484027476
1103	34-35	0.3294107520101832
1103	36-37	0.30458484071558445
1103	38-39	0.24455451733170008
1103	40-41	0.23988990602020266
1103	42-43	0.3003794948795502
1103	44-45	0.3179668754068672
1103	46-47	0.43144584132877384
1103	48-49	0.39279487079230435
1103	50-51	0.3854939703706499
1103	52-53	0.3529499717350326
1103	54-55	0.4049085156861949
1103	56-57	0.32952463192383163
1103	58-59	0.33863573219896637
1103	60-61	0.35562146627820823
1103	62-63	0.34966959787538343
1103	64-65	0.28653036112027763
1103	66-67	0.32474502277982253
1103	68-69	0.37094200964877544
1103	70-71	0.37433949097703234
1103	72-73	0.4704808420566913
1103	74-75	0.4242004840742162
1103	76-77	0.3936819759938821
1103	78-79	0.37004320018021986
1103	80-81	0.35524226693253524
1103	82-83	0.42438698280072984
1103	84-85	0.42502316918885086
1103	86-87	0.5099154860680883
1103	88-89	0.517563175200852
1103	90-91	0.45539434910734045
1103	92-93	0.4608433475591944
1103	94-95	0.49477419271341105
1103	96-97	0.48132558016127547
1103	98-99	0.3565293158473075
1103	100	0.32130768611927607
1104	1	0.0666121949484868
1104	2	0.14987492556755555
1104	3	0.09478962798452173
1104	4	0.10734241935244881
1104	5	0.14104593059007442
1104	6	0.1363529194836275
1104	7	0.10197789163589022
1104	8	0.2763457078022782
1104	9	0.1739993438243701
1104	10-11	0.12520380197915415
1104	12-13	0.2288548885378674
1104	14-15	0.28002269871940655
1104	16-17	0.3210131298592245
1104	18-19	0.24689408501974697
1104	20-21	0.2108286645436408
1104	22-23	0.31157798652363766
1104	24-25	0.27691610859920246
1104	26-27	0.27329870731581707
1104	28-29	0.18053877827859566
1104	30-31	0.2165699957874807
1104	32-33	0.2574402500776287
1104	34-35	0.284067809965201
1104	36-37	0.3392423411464094
1104	38-39	0.2590221956378045
1104	40-41	0.23062562685988297
1104	42-43	0.18750018830901638
1104	44-45	0.1097698944892187
1104	46-47	0.19225028564901692
1104	48-49	0.23305690962770242
1104	50-51	0.3145852414872259
1104	52-53	0.2968527714425342
1104	54-55	0.1927223824124802
1104	56-57	0.23650666740573456
1104	58-59	0.2522027393391255
1104	60-61	0.25643655330621584
1104	62-63	0.27919868716700336
1104	64-65	0.19824653850184148
1104	66-67	0.21974818997513168
1104	68-69	0.2716334363305357
1104	70-71	0.26133209930209844
1104	72-73	0.13666381279273665
1104	74-75	0.16936899749520506
1104	76-77	0.19455458487786714
1104	78-79	0.2337080177637425
1104	80-81	0.28284938101211665
1104	82-83	0.3520187823731611
1104	84-85	0.39238126253727756
1104	86-87	0.36014743654806125
1104	88-89	0.2346290016292336
1104	90-91	0.22481927027677884
1104	92-93	0.1493598193036192
1104	94-95	0.18435772981850107
1104	96-97	0.18044610125805605
1104	98-99	0.2137885973597342
1104	100	0.25550594323032527
1105	1	0.2523006754197077
1105	2	0.3221915856628996
1105	3	0.15880245102477275
1105	4	0.21421415609331262
1105	5	0.1939386567331809
1105	6	0.19451248613475514
1105	7	0.14035202690955373
1105	8	0.18403557422521288
1105	9	0.21956295422856442
1105	10-11	0.163513605496604
1105	12-13	0.19621191746364275
1105	14-15	0.3356657474547262
1105	16-17	0.3087227401200465
1105	18-19	0.30560553077049235
1105	20-21	0.285188856665215
1105	22-23	0.2837100895261315
1105	24-25	0.28875871496502015
1105	26-27	0.2633996816637634
1105	28-29	0.21380087249941937
1105	30-31	0.21425562072027304
1105	32-33	0.24033057918142475
1105	34-35	0.3149525414968011
1105	36-37	0.3134839134249887
1105	38-39	0.23150336282420625
1105	40-41	0.17136558767089838
1105	42-43	0.2949255216542994
1105	44-45	0.3205798697571467
1105	46-47	0.2932841488699225
1105	48-49	0.194565013263432
1105	50-51	0.2709582297663822
1105	52-53	0.21576623309587717
1105	54-55	0.17064098313890952
1105	56-57	0.24241458525224857
1105	58-59	0.2824141343114661
1105	60-61	0.3626544434336978
1105	62-63	0.31196617733988674
1105	64-65	0.24503641000261212
1105	66-67	0.23857301344550663
1105	68-69	0.35329201578977276
1105	70-71	0.3471333376977377
1105	72-73	0.37904906853666986
1105	74-75	0.35565236936038147
1105	76-77	0.2999335112432071
1105	78-79	0.3847670088267421
1105	80-81	0.3032940355397571
1105	82-83	0.34083487643865595
1105	84-85	0.3645289724312981
1105	86-87	0.29675993460653416
1105	88-89	0.3331973866967992
1105	90-91	0.2966073495986201
1105	92-93	0.3469808137837056
1105	94-95	0.4428067707040775
1105	96-97	0.2352642623032537
1105	98-99	0.1477123789767596
1105	100	0.07555572100013919
1106	1	0.11210676774391715
1106	2	0.04945272361244335
1106	3	0.1633280817279399
1106	4	0.1960143439623394
1106	5	0.013564260597917155
1106	6	0.03940311952177211
1106	7	0.06294998718867362
1106	8	0.10387924035511276
1106	9	0.08700122583350378
1106	10-11	0.15548388193245444
1106	12-13	0.10892327012064129
1106	14-15	0.09890044954812538
1106	16-17	0.08694670684039352
1106	18-19	0.08598495911187598
1106	20-21	0.14199239933832786
1106	22-23	0.17912910455564912
1106	24-25	0.15795658130962664
1106	26-27	0.055391764326131465
1106	28-29	0.06306952521009634
1106	30-31	0.1505378268394395
1106	32-33	-0.029746245831994145
1106	34-35	0.14014544273136664
1106	36-37	0.12466044321564596
1106	38-39	0.15058884323214983
1106	40-41	0.2665682178372961
1106	42-43	0.10211989900265905
1106	44-45	0.07093547898957553
1106	46-47	0.0659525600347095
1106	48-49	0.14228039168533613
1106	50-51	0.1648390671254738
1106	52-53	0.14789183696061414
1106	54-55	0.16566413128705904
1106	56-57	0.19476294810785788
1106	58-59	0.13210716973819103
1106	60-61	0.09594416431399466
1106	62-63	0.1290169690736498
1106	64-65	0.09449796394786247
1106	66-67	0.13166547184593824
1106	68-69	0.01590775653051324
1106	70-71	0.1266858068335459
1106	72-73	0.16792118340515927
1106	74-75	0.12657795980590336
1106	76-77	0.13160286175314084
1106	78-79	0.0911864264327562
1106	80-81	0.015133139136107587
1106	82-83	0.006329039079556509
1106	84-85	-0.04933475542699384
1106	86-87	-0.005625331093952468
1106	88-89	-0.033682310028911644
1106	90-91	0.040070513849823186
1106	92-93	0.04016888410039954
1106	94-95	-0.013830709167098831
1106	96-97	-0.05137523045036829
1106	98-99	-0.03069945677203023
1106	100	-0.012497821672969422
1107	1	-0.14974436794368273
1107	2	1.3297329140726788E-4
1107	3	0.042901942725421804
1107	4	0.042194671284860874
1107	5	0.023374789063318246
1107	6	0.007674065021667786
1107	7	-0.0028218286989698527
1107	8	0.024811161497623857
1107	9	0.0017688872594092686
1107	10-11	0.0578621854173349
1107	12-13	0.08486600108892617
1107	14-15	0.14065289638356404
1107	16-17	0.1730904530601407
1107	18-19	0.12565804785739232
1107	20-21	0.12228752991776304
1107	22-23	0.025082102908477566
1107	24-25	0.1313627081302613
1107	26-27	0.12064833194231994
1107	28-29	0.1297900460104131
1107	30-31	0.15988302580900893
1107	32-33	0.21102349601560633
1107	34-35	0.15877379673774072
1107	36-37	0.12993095121719023
1107	38-39	0.12799588192132916
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2216	16-17	-0.14298159593302273
2216	18-19	-0.09127382012681551
2216	20-21	-0.16290161128658553
2216	22-23	-0.04718169725942545
2216	24-25	-0.008466852187233087
2216	26-27	0.0474837793900349
2216	28-29	0.020253125240486725
2216	30-31	0.15917639599898337
2216	32-33	0.06601754107780522
2216	34-35	0.1061846196389169
2216	36-37	0.16581756198510078
2216	38-39	0.19915360954567518
2216	40-41	0.13941758857179565
2216	42-43	0.20599954705330248
2216	44-45	0.2109642587376115
2216	46-47	0.14552121390815387
2216	48-49	0.028336742156465533
2216	50-51	-0.04437586795989645
2216	52-53	0.06147420086852762
2216	54-55	0.04299944461043381
2216	56-57	0.00536567092909479
2216	58-59	0.03424506228108015
2216	60-61	-0.035513252945335694
2216	62-63	-0.06918615141391271
2216	64-65	-0.02560139955093277
2216	66-67	-0.08979362131458046
2216	68-69	-0.17093048525955723
2216	70-71	-0.17405917927075976
2216	72-73	-0.1825373070123142
2216	74-75	-0.25045177960410214
2216	76-77	-0.32896258932906264
2216	78-79	-0.2716562912520857
2216	80-81	-0.38001342701693375
2216	82-83	-0.42086433451332184
2216	84-85	-0.46023681492755486
2216	86-87	-0.4338289849376977
2216	88-89	-0.4107341672901548
2216	90-91	-0.45381747073491496
2216	92-93	-0.42277363564862824
2216	94-95	-0.5345788966723823
2216	96-97	-0.6229253101315066
2216	98-99	-0.442017647845244
2216	100	-0.37059354293041125
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	59.0
6	470.0
7	1145.0
8	1769.0
9	2572.0
10	4020.0
11	5507.0
12	6499.0
13	7218.0
14	7458.0
15	8040.0
16	8984.0
17	9894.0
18	12034.0
19	13510.0
20	15141.0
21	17430.0
22	19062.0
23	23102.0
24	37692.0
25	60925.0
26	40504.0
27	117950.0
28	55372.0
29	123521.0
30	76820.0
31	89238.0
32	115207.0
33	257915.0
34	251293.0
35	993629.0
36	110645.0
37	12028.0
38	632.0
39	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.059674737141483	43.104329272500486	21.265701805017	21.570294185341034
2	10.598550486494766	52.08959831278287	19.429120448733393	17.882730751988973
3	8.229694077576392	58.45848281143125	14.954603194228136	18.357219916764215
4	7.243348016918362	59.15930659445188	13.8283875110426	19.76895787758716
5	7.139412962368411	58.157366287313025	15.045137566380241	19.658083183938324
6	7.1230459604617336	54.67445902709033	16.61786137915686	21.584633633291084
7	9.930005224681235	59.45886660311965	12.97866654967635	17.63246162252276
8	7.8764504942498705	57.69049608448811	11.885687393188386	22.547366028073633
9	6.66269166266175	55.82118065595543	11.719654533598423	25.7964731477844
10-11	10.203290480865352	66.53563052394315	10.274880764289092	12.986198230902394
12-13	10.318824568715389	67.74577389937923	8.947228830610987	12.9881727012944
14-15	11.745051696549789	66.50054001796337	8.543889820382208	13.210518465104629
16-17	11.497185652791755	66.43598312308984	9.417922882938072	12.648908341180324
18-19	11.853186457307176	65.01264293092652	10.691771725301416	12.442398886464884
20-21	11.049270280413491	64.7448304632851	11.207984739163086	12.997914517138328
22-23	11.546806289070382	65.78944009852697	11.700734724611879	10.963018887790776
24-25	11.579146783337688	65.80243565379178	11.142228700991291	11.476188861879248
26-27	13.413265479084366	66.15534023176002	9.896603365724962	10.534790923430656
28-29	12.939256222243936	67.23850223444414	10.4905606949211	9.331680848390821
30-31	13.369190837112916	67.05553951371418	10.168677303205161	9.406592345967745
32-33	13.982535241601143	67.27244265325535	9.421038672534783	9.323983432608726
34-35	14.07268200913082	67.35445590992501	9.216526085685468	9.356335995258707
36-37	14.61760655995964	67.24656582244278	8.863603052247758	9.272224565349829
38-39	15.11252146962406	67.33755523573663	8.696930170303249	8.85299312433607
40-41	15.457479994791177	67.38936088026708	8.747391849628553	8.405767275313192
42-43	15.66713436652726	67.50005783099469	9.003288788843482	7.829519013634554
44-45	15.873485608834454	67.01471431941562	8.954842013552721	8.15695805819721
46-47	15.824865937187353	66.48241235090865	9.38340977304992	8.309311938854076
48-49	15.66536267718473	66.5702284783406	9.891854024170568	7.872554820304107
50-51	16.049171730143758	66.44925787738775	10.021915699338143	7.479654693130355
52-53	16.32977131264853	66.50755992975002	10.119284187192639	7.0433845704088185
54-55	16.217196587755147	66.85504819283355	10.060750050950755	6.867005168460545
56-57	15.990939340483854	67.15768171870955	9.649055985208129	7.202322955598461
58-59	15.616054330444396	67.08968746952677	9.768643735042566	7.525614464986269
60-61	15.584694506318462	66.6732008287684	10.2449812250856	7.497123439827547
62-63	15.654082230899006	65.997614992841	10.631707832634715	7.7165949436252905
64-65	15.395391474960531	65.79758201099456	10.74201795813597	8.06500855590894
66-67	14.872383096191824	65.48216557981932	11.244118989593904	8.401332334394954
68-69	14.633870564730097	65.38637407394039	10.982955777070474	8.996799584259035
70-71	14.400332945070247	65.16649945629209	11.123528085061324	9.309639513576345
72-73	14.437592292176216	65.49225319040531	11.134766269596351	8.935388247822129
74-75	14.289090108165054	65.98941780793918	10.924828068792824	8.796664015102944
76-77	13.591741633709884	66.71385509012796	10.949641929004787	8.744761347157368
78-79	12.983225887762053	67.46740339071741	10.673844062734812	8.875526658785729
80-81	12.379639458296051	68.72411693725209	10.187210558883713	8.70903304556815
82-83	11.683248083116467	69.9286291166881	10.061918578564805	8.326204221630622
84-85	10.873785478447159	71.26826331519241	10.039945128542909	7.818006077817522
86-87	10.202043910554833	72.3618200683085	9.835494454895032	7.600641566241635
88-89	9.700576854546386	73.26264121489831	9.599372553273843	7.437409377281459
90-91	9.466723912761259	74.1035139942173	9.193541986440811	7.236220106580634
92-93	9.409269658440088	74.7394030279342	8.63453807252365	7.216789241102053
94-95	9.424601730416455	75.0361891034455	8.13449683536037	7.404712330777678
96-97	9.540761822754257	74.77780518646338	7.946068215188714	7.735364775593657
98-99	9.81523155630846	74.20608197373498	7.726373997812412	8.252312472144146
100	10.44215132638219	73.08819934040838	7.439848857879104	9.029800475330324
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	616438.0
1	589442.5
2	342871.0
3	71553.0
4	18357.0
5	16101.5
6	13213.0
7	8793.0
8	5050.5
9	2985.5
10	2187.5
11	2015.5
12	1963.5
13	1956.5
14	1998.5
15	1980.5
16	1894.5
17	1800.5
18	1737.0
19	1663.5
20	1563.5
21	1475.5
22	1425.0
23	1371.0
24	1324.0
25	1349.5
26	1337.0
27	1300.5
28	1324.5
29	1389.5
30	1452.0
31	1497.5
32	1619.5
33	1882.0
34	2236.0
35	2710.0
36	3407.0
37	4171.0
38	5053.5
39	6392.5
40	8293.5
41	11189.0
42	15487.0
43	21831.0
44	30759.5
45	41886.0
46	53983.5
47	65371.0
48	74151.5
49	79158.5
50	80258.5
51	79261.5
52	77821.0
53	75766.5
54	72033.0
55	65920.5
56	57054.0
57	45600.0
58	33679.5
59	23122.5
60	14965.5
61	9349.0
62	5824.5
63	3844.0
64	2784.5
65	2214.5
66	1813.0
67	1470.5
68	1245.0
69	1041.5
70	890.0
71	795.5
72	682.5
73	586.0
74	535.0
75	486.0
76	425.5
77	400.0
78	385.5
79	341.0
80	341.0
81	329.5
82	295.5
83	286.0
84	253.0
85	233.0
86	225.5
87	214.5
88	214.5
89	217.5
90	220.5
91	208.0
92	195.0
93	185.5
94	183.0
95	168.5
96	157.5
97	158.5
98	153.5
99	147.0
100	123.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015075767697575314
2	4.38712816596107E-4
3	0.0
4	0.0
5	0.0
6	0.0037091174494034503
7	1.994149166345941E-4
8	0.0
9	0.0
10-11	4.5865430825956644E-4
12-13	0.0
14-15	4.38712816596107E-4
16-17	1.5953193330767529E-4
18-19	1.994149166345941E-4
20-21	7.976596665383764E-5
22-23	3.988298332691882E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	1.1964894998075646E-4
32-33	0.0
34-35	7.976596665383764E-5
36-37	9.970745831729705E-5
38-39	5.384202749134041E-4
40-41	0.0020140906580094005
42-43	0.0011566065164806457
44-45	6.181862415672416E-4
46-47	8.574841415287547E-4
48-49	0.001176548008144105
50-51	0.0
52-53	1.395904416442159E-4
54-55	4.7859579992302583E-4
56-57	4.7859579992302583E-4
58-59	4.5865430825956644E-4
60-61	0.0
62-63	1.994149166345941E-4
64-65	1.395904416442159E-4
66-67	2.5923939162497234E-4
68-69	1.794734249711347E-4
70-71	5.783032582403229E-4
72-73	4.985372915864853E-4
74-75	3.788883416057288E-4
76-77	4.38712816596107E-4
78-79	7.577766832114576E-4
80-81	9.77133091509511E-4
82-83	3.988298332691882E-4
84-85	8.176011582018359E-4
86-87	0.0013360799414517805
88-89	0.001216430991471024
90-91	0.0012563139747979429
92-93	5.982447499037822E-4
94-95	7.77718174874917E-4
96-97	7.77718174874917E-4
98-99	2.991223749518911E-4
100	0.0010369575664998893
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2507335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.704782659950496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.62221105929417	44.618019392488556
2	1.7859734433755936	1.6325505613184979
3	0.22771048927298118	0.3122237526483778
4	0.1010908892360737	0.18481348485743507
5	0.0611565643498308	0.1397573740919147
6	0.03430445011605592	0.09407264620940696
7	0.022610712477381764	0.0723392389695755
8	0.01624880432082303	0.059411845597382495
9	0.012772605285941919	0.052539223373577915
>10	0.08389318864110949	0.7375704965479565
>50	0.008639589892273945	0.2813730399805912
>100	0.012694042933340739	1.355264140602559
>500	0.0026083319035473227	0.8202268479133055
>1k	0.0045211086328153585	5.060840581505108
>5k	0.0011302771582038396	3.508008619104631
>10k+	0.002434443109977501	41.07098875479117
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	285672	11.393451612967553	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82495	3.2901467095541683	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66448	2.650144476107102	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64254	2.5626412106878416	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45702	1.8227321040068438	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38918	1.5521659451170267	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36170	1.4425675069346537	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35971	1.4346307932525968	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30572	1.219302566270562	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27779	1.1079093938384779	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27507	1.097061222373556	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26802	1.0689437191280782	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25661	1.023437235152064	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25050	0.9990687323393165	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23019	0.9180663932023443	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20744	0.8273326061336039	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19931	0.7949077406888191	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19464	0.7762823874751479	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18122	0.7227594238504229	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15603	0.6222941888499143	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14913	0.5947749303543404	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13441	0.5360671788971159	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13152	0.5245409967156364	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11984	0.4779576721897952	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11461	0.45709887190981663	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11418	0.4553839036267591	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10727	0.4278247621478582	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10571	0.4216030167488588	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8270	0.32983227211361865	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8230	0.3282369527805419	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8166	0.3256844418476191	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8110	0.3234509947813116	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7500	0.29912237495189115	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6747	0.2690904885067213	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6735	0.26861189270679825	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6297	0.2511431460096078	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6129	0.24444280481068548	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5914	0.2358679633953979	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5800	0.23132130329612915	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5206	0.2076308111999394	No Hit
TAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5175	0.20639443871680488	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4885	0.19482837355199845	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4763	0.18996264958611433	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4679	0.18661247898665315	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4589	0.18302301048723046	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4518	0.18019131867101923	No Hit
TATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4177	0.16659122135653992	No Hit
GAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4022	0.1604093589408675	No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3909	0.15590258182492567	No Hit
TAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3692	0.1472479744429843	No Hit
TAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3680	0.14676937864306128	No Hit
AAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3525	0.14058751622738883	No Hit
TAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3441	0.13723734562792766	No Hit
TAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3380	0.1348044836449856	No Hit
AAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3341	0.1332490472952358	No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3250	0.12961969581248617	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3178	0.12674812101294802	No Hit
CAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2760	0.11007703398229596	No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2687	0.10716557619943087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0030311067328458305	0.0	0.0	0.0	0.0
2	0.007697415782095332	0.0	0.0	0.0	0.0
3	0.013360799414517805	0.0	0.0	0.0	0.0
4	0.021137981163266973	0.0	0.0	0.0	0.0
5	0.032305216494804244	0.0	0.0	0.0	0.0
6	0.053682495558032735	0.0	0.0	0.0	0.0
7	0.08279707338668348	0.0	0.0	0.0	0.0
8	0.17197542410567396	0.0	0.0	0.0	0.0
9	0.4916375354709283	0.0	0.0	0.0	0.0
10-11	1.158919729513607	0.0	0.0	0.0	0.0
12-13	1.9754639886572796	0.0	0.0	0.0	0.0
14-15	2.8534479836160704	0.0	0.0	0.0	0.0
16-17	3.776120861392674	0.0	0.0	0.0	0.0
18-19	4.791661265846008	0.0	0.0	0.0	0.0
20-21	6.1207616852155775	0.0	0.0	0.0	0.0
22-23	7.659247767051471	0.0	0.0	0.0	0.0
24-25	9.231574560240254	0.0	0.0	0.0	0.0
26-27	10.871542893151494	0.0	0.0	0.0	0.0
28-29	12.697425752841164	0.0	0.0	0.0	0.0
30-31	14.793894712912316	0.0	0.0	0.0	0.0
32-33	17.150281075324997	0.0	0.0	0.0	0.0
34-35	19.33640698191506	0.0	0.0	0.0	0.0
36-37	21.053369414138913	0.0	0.0	0.0	0.0
38-39	22.344700648297895	0.0	0.0	0.0	0.0
40-41	23.467905166242247	0.0	0.0	0.0	0.0
42-43	24.33352543636969	0.0	0.0	0.0	0.0
44-45	25.01141650397733	0.0	0.0	0.0	0.0
46-47	25.563616349630188	0.0	0.0	0.0	0.0
48-49	26.067438136507487	0.0	0.0	0.0	0.0
50-51	26.59570819216419	0.0	0.0	0.0	0.0
52-53	27.168128710363796	0.0	0.0	0.0	0.0
54-55	27.77821870631567	0.0	0.0	0.0	0.0
56-57	28.44757481549135	0.0	0.0	0.0	0.0
58-59	29.17880937329874	1.994149166345941E-5	0.0	0.0	0.0
60-61	29.95365597337412	3.988298332691882E-5	0.0	0.0	0.0
62-63	30.665706816201265	3.988298332691882E-5	0.0	0.0	0.0
64-65	31.3040339643486	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
66-67	31.857171060109636	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
68-69	32.31010216026179	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
70-71	32.70972965319751	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
72-73	33.06231516729915	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
74-75	33.38664358771365	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
76-77	33.6897742024899	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
78-79	33.976592677085435	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
80-81	34.24239281946768	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
82-83	34.50625863715858	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
84-85	34.76055253885101	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
86-87	35.0132910041937	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
88	35.205985638137705	3.988298332691882E-5	3.988298332691882E-5	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAAATA	795	0.0	65.03915	1
AGCTTTT	30	1.3706731E-4	62.674084	1
CGAAAAA	2020	0.0	61.433018	1
GTAAAAA	1075	0.0	60.77929	1
TGAAAAA	2825	0.0	60.40007	1
GAAAACA	910	0.0	59.91918	1
ATACAGC	25	0.005234306	56.406677	1
GCATGCC	50	4.5659E-7	56.39993	3
CACTTTG	25	0.0052367854	56.39993	2
TAAAATA	1010	0.0	53.98659	1
GCGATTG	35	2.9364647E-4	53.720646	1
TAAAACA	1020	0.0	53.457314	1
GATAAAA	715	0.0	51.936214	1
ATTGGGC	55	8.815332E-7	51.272663	2
TAACGAC	255	0.0	49.764645	6
GCCTGCT	260	0.0	48.80763	7
TCAAAAA	1880	0.0	48.75577	1
GAAAAAT	515	0.0	48.37466	1
TTGCTGA	940	0.0	47.49994	9
CCAGGCT	40	5.678045E-4	46.99994	3
>>END_MODULE