##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248470_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2507335 Sequences flagged as poor quality 0 Sequence length 100 %GC 24 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34116462299613 34.0 31.0 34.0 30.0 34.0 2 32.52181499480524 34.0 31.0 34.0 31.0 34.0 3 32.67725852349207 34.0 31.0 34.0 31.0 34.0 4 36.21675204948681 37.0 37.0 37.0 35.0 37.0 5 36.1550199714039 37.0 37.0 37.0 35.0 37.0 6 36.13463617745535 37.0 37.0 37.0 35.0 37.0 7 36.125376545216334 37.0 37.0 37.0 35.0 37.0 8 36.134502170631364 37.0 37.0 37.0 35.0 37.0 9 37.89882205608744 39.0 38.0 39.0 35.0 39.0 10-11 37.99943386105167 39.0 38.5 39.0 35.0 39.0 12-13 37.986127900739234 39.0 38.5 39.0 35.0 39.0 14-15 39.54491322459903 41.0 40.0 41.0 36.0 41.0 16-17 39.3998610078031 41.0 39.5 41.0 36.0 41.0 18-19 38.796964705553904 40.5 38.0 41.0 35.0 41.0 20-21 37.918673212793664 39.5 35.5 41.0 34.5 41.0 22-23 37.03301872306652 38.0 35.0 40.5 34.0 41.0 24-25 36.273811636658046 35.0 35.0 40.0 33.0 41.0 26-27 36.0219571776408 35.0 35.0 40.0 33.0 41.0 28-29 35.85968847401723 35.0 35.0 40.0 33.0 41.0 30-31 35.78202553707422 35.0 35.0 40.0 33.0 41.0 32-33 35.773915531829616 35.0 35.0 40.0 33.0 41.0 34-35 35.73577064891608 35.0 35.0 40.0 33.0 41.0 36-37 35.68502812747399 35.0 35.0 40.0 33.0 41.0 38-39 35.57861893205336 35.0 35.0 40.0 33.0 41.0 40-41 35.51177405492285 35.0 35.0 39.5 33.0 41.0 42-43 35.42461617613921 35.0 35.0 39.0 33.0 41.0 44-45 35.29144789188521 35.0 35.0 39.0 32.5 41.0 46-47 35.11318112657463 35.0 35.0 38.5 32.0 41.0 48-49 34.882283978806186 35.0 35.0 37.5 31.5 40.5 50-51 34.62357802208321 35.0 35.0 36.5 31.0 40.0 52-53 34.35527482366736 35.0 35.0 35.5 31.0 39.0 54-55 34.08782472226488 35.0 35.0 35.0 31.0 38.5 56-57 33.817607938308996 35.0 35.0 35.0 30.0 37.0 58-59 33.617196345921066 35.0 34.0 35.0 30.0 36.5 60-61 33.447001497606024 35.0 34.0 35.0 30.0 35.5 62-63 33.28994131219004 35.0 34.0 35.0 29.0 35.0 64-65 33.16750513991948 35.0 34.0 35.0 29.0 35.0 66-67 33.06380559438607 35.0 33.5 35.0 29.0 35.0 68-69 32.977230007158994 35.0 33.0 35.0 29.0 35.0 70-71 32.91733872817154 35.0 33.0 35.0 29.0 35.0 72-73 32.866191593863604 35.0 33.0 35.0 29.0 35.0 74-75 32.82720617707646 35.0 33.0 35.0 29.0 35.0 76-77 32.78263275549538 35.0 33.0 35.0 29.0 35.0 78-79 32.72835181577253 35.0 33.0 35.0 28.5 35.0 80-81 32.67090695898234 35.0 33.0 35.0 27.0 35.0 82-83 32.59171470904367 35.0 33.0 35.0 27.0 35.0 84-85 32.48463707482247 35.0 33.0 35.0 27.0 35.0 86-87 32.38894064813836 35.0 33.0 35.0 27.0 35.0 88-89 32.27913681259186 35.0 33.0 35.0 26.0 35.0 90-91 32.165571413472875 35.0 33.0 35.0 25.0 35.0 92-93 31.987213714960305 35.0 33.0 35.0 24.5 35.0 94-95 31.762445983484454 35.0 33.0 35.0 24.0 35.0 96-97 31.526257959147863 35.0 33.0 35.0 21.5 35.0 98-99 31.133772910281238 35.0 32.5 35.0 19.5 35.0 100 29.8934546041913 34.0 29.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2986956517232713 1101 2 0.2855905270934187 1101 3 0.26064277432521976 1101 4 0.15285401641488505 1101 5 0.20656596282328366 1101 6 0.11929478160433149 1101 7 0.1720253830712295 1101 8 0.19280369820674537 1101 9 0.229373989515544 1101 10-11 0.27996428683361074 1101 12-13 0.3141055583766743 1101 14-15 0.39617890364427666 1101 16-17 0.4279188374956817 1101 18-19 0.40582994587916943 1101 20-21 0.47127566362426165 1101 22-23 0.4411532116488672 1101 24-25 0.4459894011243648 1101 26-27 0.4752638752323719 1101 28-29 0.5192368785883161 1101 30-31 0.5258536916094485 1101 32-33 0.5192058158468384 1101 34-35 0.4286848905660179 1101 36-37 0.44209311196838996 1101 38-39 0.5896508492013055 1101 40-41 0.5233111732515212 1101 42-43 0.5379217173954416 1101 44-45 0.5229511664540354 1101 46-47 0.4384169853696349 1101 48-49 0.42093433454362383 1101 50-51 0.42491944753248134 1101 52-53 0.4370707753326428 1101 54-55 0.4834162408422529 1101 56-57 0.4847764893125657 1101 58-59 0.4828739901209502 1101 60-61 0.4763535387212059 1101 62-63 0.514051588433496 1101 64-65 0.4314993847595048 1101 66-67 0.486146341335953 1101 68-69 0.4853639693804297 1101 70-71 0.511297610514319 1101 72-73 0.47523053861258546 1101 74-75 0.44577317195278 1101 76-77 0.4334989921586043 1101 78-79 0.42237999032665385 1101 80-81 0.4123712005593987 1101 82-83 0.43757624375844983 1101 84-85 0.42395970504631464 1101 86-87 0.46545606010107576 1101 88-89 0.5272626733447083 1101 90-91 0.5250636331229686 1101 92-93 0.5321563066249482 1101 94-95 0.5238078068030241 1101 96-97 0.5279257104051744 1101 98-99 0.7307721014420352 1101 100 1.0658476163069857 1102 1 0.27485430716969006 1102 2 0.2496771018536279 1102 3 0.2603620576316885 1102 4 0.1654530723336194 1102 5 0.09738602179904632 1102 6 0.12110663106008701 1102 7 0.2250088548836473 1102 8 0.19797557854854375 1102 9 0.16958097297472818 1102 10-11 0.23696880878986093 1102 12-13 0.29208589557948983 1102 14-15 0.37962342917262504 1102 16-17 0.4070317384663724 1102 18-19 0.4671198174368456 1102 20-21 0.4567687354604928 1102 22-23 0.38730106960549904 1102 24-25 0.2919184678405884 1102 26-27 0.32640591048252077 1102 28-29 0.39018103202271703 1102 30-31 0.3533152621977038 1102 32-33 0.35792979069418607 1102 34-35 0.3594066806581253 1102 36-37 0.3611881614501229 1102 38-39 0.3374244645382447 1102 40-41 0.3443246489156806 1102 42-43 0.3434058409360503 1102 44-45 0.39697025915819495 1102 46-47 0.40764711622944105 1102 48-49 0.4059193741118676 1102 50-51 0.41886112665489605 1102 52-53 0.42281520226737257 1102 54-55 0.39391095891230776 1102 56-57 0.3996129799569985 1102 58-59 0.3702726339851026 1102 60-61 0.487648461736093 1102 62-63 0.4382682742987143 1102 64-65 0.42304586132299704 1102 66-67 0.45176495090431956 1102 68-69 0.5190396441784415 1102 70-71 0.5199604086066714 1102 72-73 0.47797045802380467 1102 74-75 0.4640536739593841 1102 76-77 0.4773131670887665 1102 78-79 0.473498121359647 1102 80-81 0.40716089306095427 1102 82-83 0.4240748712955664 1102 84-85 0.4328822021620198 1102 86-87 0.47749575733837446 1102 88-89 0.4732464491466004 1102 90-91 0.45727821902525534 1102 92-93 0.4422834860806688 1102 94-95 0.43846898482089003 1102 96-97 0.5302307985756549 1102 98-99 0.6891573851039077 1102 100 1.1192490059989453 1103 1 0.20734183968455966 1103 2 0.26159286970160167 1103 3 0.2114340128315817 1103 4 0.1466557497364036 1103 5 0.17051838424250576 1103 6 0.2117663722665739 1103 7 0.2283887445401902 1103 8 0.18019636486993562 1103 9 0.22591412405910916 1103 10-11 0.2315568433177546 1103 12-13 0.2284632006145273 1103 14-15 0.312406287830278 1103 16-17 0.32203455504108547 1103 18-19 0.3220525510054486 1103 20-21 0.25344186368091925 1103 22-23 0.18150472452538935 1103 24-25 0.23068654776503195 1103 26-27 0.1592624971253258 1103 28-29 0.23419042612780316 1103 30-31 0.24303932071618561 1103 32-33 0.2384096853211588 1103 34-35 0.2806987428878642 1103 36-37 0.26032496414077855 1103 38-39 0.23142930241952087 1103 40-41 0.2629152846331593 1103 42-43 0.27083140880721146 1103 44-45 0.32361754116359975 1103 46-47 0.29070459092168477 1103 48-49 0.3427966211586124 1103 50-51 0.34985297515631686 1103 52-53 0.3426587995303265 1103 54-55 0.3174645649563459 1103 56-57 0.31088549184460135 1103 58-59 0.32933579153103665 1103 60-61 0.38212590428625504 1103 62-63 0.36842799065306764 1103 64-65 0.40760647566764874 1103 66-67 0.4000849469279757 1103 68-69 0.3960514240879789 1103 70-71 0.46454015909129254 1103 72-73 0.42654643098468625 1103 74-75 0.4947982437690115 1103 76-77 0.41237372344511414 1103 78-79 0.40374730538139403 1103 80-81 0.3916925132541351 1103 82-83 0.3568821678808902 1103 84-85 0.3809437360354764 1103 86-87 0.4714140269674161 1103 88-89 0.49685555828058625 1103 90-91 0.49587191983874845 1103 92-93 0.4474038532294671 1103 94-95 0.46836941062071347 1103 96-97 0.5015597247979677 1103 98-99 0.7015039232022247 1103 100 1.029073881324095 1104 1 0.17532219375836178 1104 2 0.2059513030577591 1104 3 0.18952051452463792 1104 4 0.12896114188642116 1104 5 0.09825160392941967 1104 6 0.12535806489957935 1104 7 0.18499351116913232 1104 8 0.1567768186172387 1104 9 0.17723107181436148 1104 10-11 0.18800720917538882 1104 12-13 0.1658215501331597 1104 14-15 0.2148523640013238 1104 16-17 0.21616185300430146 1104 18-19 0.16806316576737146 1104 20-21 0.27269352063640184 1104 22-23 0.23091646457317694 1104 24-25 0.21834950695013333 1104 26-27 0.23437940389334244 1104 28-29 0.2108685738774554 1104 30-31 0.27667481845490016 1104 32-33 0.29509095368972993 1104 34-35 0.2686106529395147 1104 36-37 0.2584195235513107 1104 38-39 0.23360465325626478 1104 40-41 0.20049114594726092 1104 42-43 0.2601999958189154 1104 44-45 0.28979771731555104 1104 46-47 0.3019066812705944 1104 48-49 0.2857202684634643 1104 50-51 0.3053670389007763 1104 52-53 0.2542828720777308 1104 54-55 0.23910081723276022 1104 56-57 0.24437310950823132 1104 58-59 0.19559899011883886 1104 60-61 0.24666950212697714 1104 62-63 0.19745882888857125 1104 64-65 0.16041328606908678 1104 66-67 0.1695510882128346 1104 68-69 0.23254054151174586 1104 70-71 0.1350237618691068 1104 72-73 0.17506623294182333 1104 74-75 0.26790805695107167 1104 76-77 0.20594781019832453 1104 78-79 0.2215159697099196 1104 80-81 0.2667057875285934 1104 82-83 0.24762860129293784 1104 84-85 0.2502254347699875 1104 86-87 0.21082546426859494 1104 88-89 0.18509979095766482 1104 90-91 0.14540126530057762 1104 92-93 0.15044692242802427 1104 94-95 0.2679499901243041 1104 96-97 0.28104887482521335 1104 98-99 0.5376509369562328 1104 100 0.8955477216881356 1105 1 0.09132918923012312 1105 2 0.14273540224888848 1105 3 0.14379553334251227 1105 4 0.023048220873718606 1105 5 0.056645871346411525 1105 6 0.07004597187354733 1105 7 0.10306173643407845 1105 8 0.11444010105268632 1105 9 0.16845895333938898 1105 10-11 0.06891329750590813 1105 12-13 0.10417709155041166 1105 14-15 0.22690364720143918 1105 16-17 0.16063686405607314 1105 18-19 0.184761344913575 1105 20-21 0.17411860158315307 1105 22-23 0.20359465943082 1105 24-25 0.20919382484683524 1105 26-27 0.2485303367224816 1105 28-29 0.2385175263488719 1105 30-31 0.2274127854668606 1105 32-33 0.2638195968907979 1105 34-35 0.19758802070657566 1105 36-37 0.223834390571632 1105 38-39 0.20947677232862816 1105 40-41 0.21844448507530956 1105 42-43 0.2559755687187817 1105 44-45 0.20633370426848785 1105 46-47 0.19053034012335957 1105 48-49 0.26729536225415984 1105 50-51 0.19157644604799628 1105 52-53 0.20049394445786106 1105 54-55 0.24326826797771162 1105 56-57 0.18841788560534667 1105 58-59 0.22705057291153707 1105 60-61 0.21378044248326233 1105 62-63 0.23266973101170407 1105 64-65 0.2264071388953539 1105 66-67 0.23522910683954734 1105 68-69 0.16116518606292374 1105 70-71 0.17686744354228523 1105 72-73 0.19362454447031752 1105 74-75 0.1293874110497768 1105 76-77 0.1400135846585755 1105 78-79 0.14055951368401765 1105 80-81 0.16609760414089436 1105 82-83 0.17645199102016562 1105 84-85 0.2469441044953129 1105 86-87 0.24865395573184657 1105 88-89 0.1991046985409639 1105 90-91 0.21040919569234973 1105 92-93 0.1967651895104794 1105 94-95 0.23620900777129705 1105 96-97 0.30467935142533165 1105 98-99 0.42628284729949684 1105 100 0.8951909287325925 1106 1 0.11013622089947717 1106 2 0.05672829006423541 1106 3 0.04326211949388181 1106 4 0.024742396933838506 1106 5 0.05998725620953138 1106 6 0.06751980440172645 1106 7 0.10295773750761583 1106 8 0.14492520572257916 1106 9 0.14553893186864286 1106 10-11 0.11484559755958657 1106 12-13 0.08393755853914087 1106 14-15 0.05642927790460561 1106 16-17 0.09406413190362883 1106 18-19 0.07919907974986273 1106 20-21 0.05271495155631811 1106 22-23 0.01163109260312467 1106 24-25 0.09235103096600028 1106 26-27 0.1602402803617622 1106 28-29 0.15438407224259265 1106 30-31 0.2005961590578451 1106 32-33 0.21117936608028032 1106 34-35 0.18852669488800444 1106 36-37 0.14383070028713973 1106 38-39 0.15597100206561265 1106 40-41 0.15055163751760148 1106 42-43 0.23091015003922877 1106 44-45 0.17966301183692224 1106 46-47 0.20274685917864588 1106 48-49 0.22825382977966058 1106 50-51 0.21845178152839395 1106 52-53 0.21573985159688647 1106 54-55 0.16888722933037315 1106 56-57 0.14861145780073315 1106 58-59 0.1999588525680025 1106 60-61 0.22795616712094358 1106 62-63 0.19035726455974356 1106 64-65 0.16239076745144843 1106 66-67 0.16634994688249094 1106 68-69 0.14867021826904647 1106 70-71 0.18804061852886633 1106 72-73 0.13835079245743742 1106 74-75 0.1469598748178953 1106 76-77 0.1482680800960452 1106 78-79 0.2119161964537426 1106 80-81 0.18851943720584785 1106 82-83 0.1760393501183941 1106 84-85 0.1915831275763651 1106 86-87 0.16552526544735713 1106 88-89 0.19436436037671 1106 90-91 0.16194905076481092 1106 92-93 0.11850431994526645 1106 94-95 0.051896071646762465 1106 96-97 0.12824349527933165 1106 98-99 0.2383198843365335 1106 100 0.4964456254583034 1107 1 0.12837976070792223 1107 2 0.13221583128800063 1107 3 0.09701081391227007 1107 4 0.0675190295578787 1107 5 -0.027919920394445796 1107 6 0.030741437383859704 1107 7 0.07954966073480563 1107 8 0.1032557204886757 1107 9 0.10636028008684661 1107 10-11 0.10961511675811408 1107 12-13 0.09245856590570156 1107 14-15 0.12257484644329963 1107 16-17 0.06317692493712457 1107 18-19 0.050181912370938164 1107 20-21 0.1049367625158979 1107 22-23 0.1265864103354204 1107 24-25 0.11862231114859867 1107 26-27 0.159298134379128 1107 28-29 0.1855422162001119 1107 30-31 0.16896141133556597 1107 32-33 0.12943077660477798 1107 34-35 0.10286306234691978 1107 36-37 0.1145853885039756 1107 38-39 0.11957347843396349 1107 40-41 0.21294383175269616 1107 42-43 0.22811764125912504 1107 44-45 0.23430237350058292 1107 46-47 0.23469476921990662 1107 48-49 0.26562274867653457 1107 50-51 0.21314865614505862 1107 52-53 0.17445096157347706 1107 54-55 0.2510645246545309 1107 56-57 0.18203704159868295 1107 58-59 0.15312263224183909 1107 60-61 0.19054208024099495 1107 62-63 0.1864179002725166 1107 64-65 0.18447603212376862 1107 66-67 0.17457725599812335 1107 68-69 0.1684972965103384 1107 70-71 0.15734404597753127 1107 72-73 0.11062385525085716 1107 74-75 0.06917188638146854 1107 76-77 0.18123124159959048 1107 78-79 0.233950904184276 1107 80-81 0.18334873130189067 1107 82-83 0.1634950241601345 1107 84-85 0.12434991116925431 1107 86-87 0.19005343020201337 1107 88-89 0.3013098418411815 1107 90-91 0.301517905703264 1107 92-93 0.28429606079874503 1107 94-95 0.21939636059422085 1107 96-97 0.2062071212260328 1107 98-99 0.35964824848265664 1107 100 0.7504196634405353 1108 1 0.11378709534049136 1108 2 0.11059301741619265 1108 3 0.09396091224379433 1108 4 0.07546967468861965 1108 5 0.04293356169745266 1108 6 0.08287961378285758 1108 7 0.11566788563102648 1108 8 0.06826351212090742 1108 9 0.1258743434957168 1108 10-11 0.11231731021286606 1108 12-13 0.13070250264114236 1108 14-15 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-0.34847941381461567 2214 90-91 -0.24697883823186828 2214 92-93 -0.28507820251899574 2214 94-95 -0.2729249056641443 2214 96-97 -0.2231194942498682 2214 98-99 -0.09412337115990255 2214 100 0.2210435661393788 2215 1 -0.30697960835485105 2215 2 -0.22022207020965112 2215 3 -0.29740761677313543 2215 4 -0.20397646842725692 2215 5 -0.18171908729239306 2215 6 -0.19147954073083184 2215 7 -0.19949711995828778 2215 8 -0.1826736814383949 2215 9 -0.24910485267464821 2215 10-11 -0.24219897069497875 2215 12-13 -0.2933552035058469 2215 14-15 -0.35699366530101173 2215 16-17 -0.38518781152318127 2215 18-19 -0.4485927343587761 2215 20-21 -0.430174337610012 2215 22-23 -0.44122698266743043 2215 24-25 -0.40405119122718247 2215 26-27 -0.394696890117217 2215 28-29 -0.47374197594461265 2215 30-31 -0.47261355534154603 2215 32-33 -0.5045672674993682 2215 34-35 -0.534928788404649 2215 36-37 -0.5437386040965038 2215 38-39 -0.43717439205761366 2215 40-41 -0.4816690866893012 2215 42-43 -0.45657723541341255 2215 44-45 -0.4298470500342546 2215 46-47 -0.43343967301029096 2215 48-49 -0.36315595993190186 2215 50-51 -0.42720491789132353 2215 52-53 -0.41738778898785966 2215 54-55 -0.4582728530870881 2215 56-57 -0.4364447746447837 2215 58-59 -0.3947222492549258 2215 60-61 -0.49967579011955365 2215 62-63 -0.4397051317710563 2215 64-65 -0.39692616176721174 2215 66-67 -0.5247763573467523 2215 68-69 -0.5484122093579415 2215 70-71 -0.45184121976583214 2215 72-73 -0.5363427194180233 2215 74-75 -0.48742807097476515 2215 76-77 -0.490314732078005 2215 78-79 -0.5340567681209833 2215 80-81 -0.59701607543726 2215 82-83 -0.5765243978142749 2215 84-85 -0.5542815394455687 2215 86-87 -0.5745835378973503 2215 88-89 -0.6460498178662917 2215 90-91 -0.7008859879827227 2215 92-93 -0.6542737409791926 2215 94-95 -0.7049493017123787 2215 96-97 -0.6594697056962424 2215 98-99 -0.4813875961081955 2215 100 0.10274130706011064 2216 1 -0.3341295745007926 2216 2 -0.3804125871246882 2216 3 -0.3225649282620964 2216 4 -0.26799513709763545 2216 5 -0.27818818031153825 2216 6 -0.2523805363522058 2216 7 -0.24150226526533203 2216 8 -0.371313973434674 2216 9 -0.42354358553716054 2216 10-11 -0.42077248452948623 2216 12-13 -0.4479102514333846 2216 14-15 -0.55797423334851 2216 16-17 -0.5860275333190543 2216 18-19 -0.7431098634073834 2216 20-21 -0.748087098604195 2216 22-23 -0.6909359747252637 2216 24-25 -0.5276536365325555 2216 26-27 -0.598306720893639 2216 28-29 -0.6012664068111064 2216 30-31 -0.6442584261812598 2216 32-33 -0.7138524299107303 2216 34-35 -0.7065992700664481 2216 36-37 -0.7755393681429581 2216 38-39 -0.8125466380582367 2216 40-41 -0.7861535495987866 2216 42-43 -0.751038365669686 2216 44-45 -0.7610428838090328 2216 46-47 -0.6739206214249478 2216 48-49 -0.6921787486271782 2216 50-51 -0.6507088371698373 2216 52-53 -0.6600411455289361 2216 54-55 -0.618081463546325 2216 56-57 -0.6583958819134068 2216 58-59 -0.6382692244390284 2216 60-61 -0.6587575574860267 2216 62-63 -0.7068737510881888 2216 64-65 -0.6605318512869758 2216 66-67 -0.670507455286149 2216 68-69 -0.6968849657066158 2216 70-71 -0.6995956416534028 2216 72-73 -0.7334654218486207 2216 74-75 -0.725250005643332 2216 76-77 -0.8045007336325938 2216 78-79 -0.788113283482268 2216 80-81 -0.807767291539939 2216 82-83 -0.8462763926982646 2216 84-85 -0.8896941619024545 2216 86-87 -0.8299983026397904 2216 88-89 -0.93600928217176 2216 90-91 -0.9386675822890425 2216 92-93 -0.9611478130181581 2216 94-95 -0.9336129125612196 2216 96-97 -1.0380270090746642 2216 98-99 -0.9419640482073071 2216 100 -0.44527996632376343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 15.0 8 25.0 9 92.0 10 239.0 11 464.0 12 769.0 13 1036.0 14 1500.0 15 2353.0 16 3118.0 17 4445.0 18 5942.0 19 7443.0 20 9196.0 21 11027.0 22 13271.0 23 15971.0 24 18711.0 25 21119.0 26 24790.0 27 28873.0 28 34638.0 29 42371.0 30 48271.0 31 59738.0 32 78194.0 33 117057.0 34 173825.0 35 1107143.0 36 287797.0 37 358277.0 38 28455.0 39 1160.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.18269575970322 21.46671187522438 34.1682157246001 26.1823766404723 2 14.275560893628558 20.342463647576444 44.53412957222789 20.8478458865671 3 9.711107610271464 14.478878969104647 54.82095531709963 20.98905810352426 4 9.855527823576756 11.416029934583904 61.058558612454505 17.669883629384834 5 8.76100337098242 12.373129332175226 63.06697558361803 15.798891713224315 6 8.163511933796276 8.38055893791347 58.20425311621188 25.251676012078374 7 11.00570924906325 8.546723912042069 56.94221155130846 23.505355287586223 8 8.857930830942017 7.223964887021479 57.697595255520305 26.220509026516204 9 7.969417728384919 7.404196088675826 63.27180053722379 21.35458564571547 10-11 9.868126915629542 10.822327291726076 66.01108348106655 13.298462311577829 12-13 9.264796763547917 16.698051520083148 60.947185104694725 13.089966611674209 14-15 11.3618039996929 13.839312332801889 60.699811651296656 14.099072016208558 16-17 16.065404104357814 14.880400903748402 61.87338349283203 7.180811499061752 18-19 15.957420927000182 10.18595440976176 60.91451680768625 12.94210785555181 20-21 11.696692085573442 9.642726097985099 64.87043935357273 13.79014246286872 22-23 11.877758551822238 10.811180000610214 66.60058672289676 10.710474724670778 24-25 11.868762255627791 12.252746167988125 64.46843604379511 11.410055532588977 26-27 15.563469374080679 11.62836100200695 61.5099462048237 11.298223419088673 28-29 13.486477507182123 14.280868371816801 63.81209101384096 8.420563107160108 30-31 13.56712919708917 12.55129855976159 63.88409890723275 9.9974733359165 32-33 14.10513817313211 13.43382578463362 63.42295731623414 9.038078726000128 34-35 14.109881098647461 13.577883147907475 63.57801277217856 8.734222981266502 36-37 14.298064123720591 12.637767286034071 63.30234668288621 9.761821907359122 38-39 14.791609334306488 12.587115274936087 62.52102851262907 10.100246878128353 40-41 14.863198626871135 13.043986349277189 62.68348418486826 9.409330838983411 42-43 14.880983244323371 13.230882135920009 62.77687150258654 9.111263117170083 44-45 15.203236905412174 12.139890982385 62.689396318895575 9.96747579330726 46-47 15.320016713086368 11.525128963000856 63.24806268803469 9.90679163587809 48-49 14.373498452160563 11.49367752012251 63.70764730200508 10.425176725711843 50-51 14.356551153911045 11.324585618281018 63.78317536631955 10.535687861488396 52-53 13.914732655118103 11.707628266155938 63.924544135108064 10.453094943617891 54-55 13.736050294518709 11.922174792038163 64.01714825165287 10.32462666179026 56-57 13.64199743019231 11.830847384413994 63.883991310595945 10.643163874797757 58-59 13.323717323614423 11.90018095328119 64.39300484150733 10.383096881597046 60-61 12.759895204254375 11.396517392296364 64.7775483730971 11.066039030352155 62-63 12.589020244801185 11.033524344100194 65.01746699459994 11.359988416498672 64-65 12.44257497835624 10.906620807088425 65.19892615832762 11.45187805622771 66-67 12.109046938200393 10.557131235539416 65.91782589003205 11.41599593622814 68-69 12.044982111699023 9.88542273488479 66.51005719589539 11.559537957520796 70-71 11.698086190147697 9.527268245490491 67.23183717409562 11.5428083902662 72-73 11.33546600106055 9.026385531044037 68.08943563677003 11.548712831125382 74-75 11.058483384176226 8.555086712107189 68.74912129245241 11.637308611264169 76-77 11.331228565286562 8.367130999875165 68.97633452092131 11.325305913916955 78-79 11.626082836007235 8.468457652227258 68.97135864358212 10.934100868183389 80-81 11.63711449911883 8.48055571675947 68.90733460528207 10.974995178839624 82-83 11.579733571594533 8.726203380264275 68.67193646722596 11.022126580915238 84-85 11.2599412311152 9.186477333055723 68.33446411783453 11.21911731799455 86-87 11.280593296004044 9.543020322107225 68.02631996175732 11.150066420131411 88-89 11.16094532686395 10.089903017063005 67.64428727243791 11.104864383635134 90-91 11.102294274325363 10.652569122981113 67.27047484881528 10.974661753878234 92-93 10.846992846330728 11.002371260876588 66.94404502404167 11.20659086875101 94-95 10.635472460879116 11.564673874134739 66.72876631686256 11.071087348123578 96-97 10.090671157214924 12.372307346226377 67.00866945969834 10.52835203686036 98-99 9.815720263359779 13.112024052511712 66.81869077259279 10.25356491153571 100 9.68310709529517 13.795940500945122 66.35324955970748 10.167702844052226 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 170943.0 1 362157.0 2 485411.5 3 270910.0 4 74781.5 5 18153.0 6 8956.5 7 5884.5 8 4575.5 9 4173.5 10 4219.5 11 4505.0 12 5034.0 13 5659.5 14 6260.5 15 6539.5 16 6790.0 17 7385.0 18 7776.5 19 7249.5 20 5769.5 21 4184.0 22 3081.0 23 2513.5 24 2287.5 25 2186.5 26 2120.5 27 1903.5 28 1691.5 29 1627.0 30 1558.0 31 1489.5 32 1429.5 33 1365.0 34 1357.0 35 1359.5 36 1321.5 37 1363.0 38 1436.5 39 1510.5 40 1731.0 41 2212.5 42 3142.5 43 5000.5 44 8480.0 45 13913.5 46 21300.5 47 30224.5 48 40689.0 49 53649.5 50 70264.0 51 90307.0 52 110307.5 53 124076.5 54 126837.0 55 116040.0 56 94254.5 57 68521.5 58 44723.0 59 26795.5 60 15024.5 61 7909.0 62 3940.5 63 1889.5 64 916.0 65 461.5 66 317.0 67 229.5 68 167.0 69 143.5 70 111.5 71 78.0 72 55.0 73 40.5 74 39.0 75 30.0 76 20.5 77 16.5 78 9.0 79 4.5 80 5.5 81 3.0 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 1.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017349097747209684 2 0.11542135374810307 3 0.0 4 3.988298332691882E-5 5 0.03764953626061136 6 0.001754851266384428 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.574841415287547E-4 14-15 6.979522082210793E-4 16-17 0.0 18-19 0.0 20-21 5.982447499037822E-4 22-23 7.378351915479982E-4 24-25 0.002731984357893939 26-27 0.0018745002163651843 28-29 0.0027120428662304797 30-31 0.0030510482245092894 32-33 0.0032704046328073436 34-35 0.0035296440244323157 36-37 0.0028915162912016147 38-39 9.970745831729706E-4 40-41 0.002731984357893939 42-43 0.004985372915864853 44-45 0.005005314407528312 46-47 0.012662847206296724 48-49 0.0052047293241629065 50-51 0.006620575232268524 52-53 0.0027120428662304797 54-55 9.571915998460517E-4 56-57 0.005723208107412851 58-59 0.0023132130329612914 60-61 0.014477522947671531 62-63 0.01280243764794094 64-65 0.01042940013998927 66-67 0.01214436842304678 68-69 0.009950804340066245 70-71 0.008594782906951006 72-73 0.005842857057393607 74-75 0.00404812280768226 76-77 4.7859579992302583E-4 78-79 0.010409458648325813 80-81 0.006321452857316633 82-83 0.011266942789854567 84-85 0.009013554231883653 86-87 0.007517942357124197 88-89 0.009751389423431652 90-91 0.005463968715787878 92-93 0.00917308616519133 94-95 0.0029712822578554523 96-97 0.005703266615749391 98-99 0.44389760442860643 100 1.740174328520122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2507335.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.23178026556068 #Duplication Level Percentage of deduplicated Percentage of total 1 97.13624789491864 45.87917916393828 2 2.0533738962358092 1.9396900934009587 3 0.25251200624425124 0.35779774780033086 4 0.1189264528752408 0.22468432359863724 5 0.0691695895967988 0.16335014284475063 6 0.047659626392191325 0.13506294007768183 7 0.03650106603242464 0.12068072312115409 8 0.02499300831438573 0.09443714215043182 9 0.02432644585670296 0.10340832108112678 >10 0.17099877192488422 1.6897629090538768 >50 0.030828998579219065 1.00974720958389 >100 0.01879020540177479 1.7057575360070316 >500 0.0029490393385703632 1.0444638518566822 >1k 0.007414727479834056 8.547650199784103 >5k 0.0021907149372236982 7.58405320054593 >10k+ 0.003117555872202955 29.400274495155127 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35983 1.43510938905252 No Hit TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29718 1.1852424985093735 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29314 1.1691297732452983 No Hit TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29069 1.159358442330203 No Hit TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25956 1.0352027152335048 No Hit CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25615 1.0216026179190256 No Hit TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24736 0.986545475574664 No Hit TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24170 0.9639717070116278 No Hit TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23659 0.9435915025315723 No Hit GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23599 0.9411985235319572 No Hit CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22635 0.9027513276048074 No Hit CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21765 0.8680531321103881 No Hit ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21078 0.840653522564795 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20770 0.828369563700104 No Hit TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20650 0.8235836057008736 No Hit CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20441 0.8152480621855476 No Hit CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20251 0.8076702953534329 No Hit TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19049 0.7597309493944766 No Hit ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18847 0.751674586762439 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18842 0.7514751718458044 No Hit GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18629 0.7429800963971708 No Hit GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18604 0.7419830218139977 No Hit TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17910 0.7143042313851161 No Hit GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17518 0.6986701019209639 No Hit TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16685 0.6654475768096405 No Hit ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16553 0.6601830230104873 No Hit TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16522 0.6589466505273528 No Hit ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16505 0.6582686398107951 No Hit CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16446 0.6559155437945069 No Hit TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15653 0.6242883380162603 No Hit AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15085 0.6016348034865704 No Hit TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14256 0.5685718103085547 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13872 0.5532567447110179 No Hit AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13422 0.5353094022139044 No Hit ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11811 0.47105791607423814 No Hit ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11787 0.47010072447439216 No Hit CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11357 0.452951041643817 No Hit TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9749 0.3888192044541316 No Hit CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9362 0.373384489906614 No Hit CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9044 0.3607017012086538 No Hit CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8814 0.35152861504346244 No Hit TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8561 0.341438220261752 No Hit TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8390 0.3346182301128489 No Hit CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8388 0.3345384641461951 No Hit ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7922 0.3159529939158509 No Hit GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7736 0.30853475901704397 No Hit TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7733 0.30841511006706324 No Hit ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7684 0.3064608438840442 No Hit TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7394 0.29489477871923775 No Hit TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7375 0.2941370020360263 No Hit ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7257 0.2894308100034499 No Hit ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7171 0.2860008734373348 No Hit CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6885 0.27459434020583606 No Hit CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6850 0.2731984357893939 No Hit CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6756 0.26944943535666355 No Hit TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6748 0.2691303714900482 No Hit ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6385 0.2546528485423767 No Hit GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6351 0.25329682710926144 No Hit GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6290 0.2508639651263194 No Hit ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5875 0.23431252704564806 No Hit TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5440 0.2169634292984384 No Hit TTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5348 0.21329419483236187 No Hit CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5062 0.20188766160086305 No Hit GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4959 0.1977797143181904 No Hit TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4795 0.19123890505257574 No Hit GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4769 0.19020194748607586 No Hit TCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4728 0.18856674516967217 No Hit TGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4700 0.18745002163651844 No Hit AACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4668 0.18617376617005704 No Hit GTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4596 0.1833021913705189 No Hit CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4397 0.17536547768846206 No Hit GCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4367 0.1741689881886545 No Hit CATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4343 0.17321179658880845 No Hit ATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4229 0.16866513648953968 No Hit CCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4155 0.16571379572334768 No Hit GTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4076 0.1625630400405211 No Hit CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3928 0.15666035850813714 No Hit GCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3906 0.1557829328749449 No Hit ACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3851 0.1535893687919644 No Hit GGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3825 0.15255241122546448 No Hit CATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3522 0.1404678672774081 No Hit ACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3438 0.1371176966779469 No Hit GTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3402 0.13568190927817783 No Hit GTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3331 0.13285021746196657 No Hit GCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3297 0.13149419602885135 No Hit AATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3260 0.13001852564575533 No Hit TTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3196 0.12746601471283256 No Hit TCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3166 0.12626952521302498 No Hit GGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3115 0.12423549306335212 No Hit TAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2898 0.11558088568141074 No Hit GCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2870 0.11446416214825701 No Hit TTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2851 0.11370638546504556 No Hit CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2754 0.10983773608233442 No Hit TCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2731 0.1089204274658153 No Hit TTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2657 0.10596908669962331 No Hit AGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2609 0.1040547034999312 No Hit TCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2533 0.10102359676708537 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.002831691816211236 0.0 0.0 0.0 0.0 2 0.00809624561536452 0.0 0.0 0.0 0.0 3 0.013998927147748506 0.0 0.0 0.0 0.0 4 0.022414236629728378 0.0 0.0 0.0 0.0 5 0.036014333944207694 0.0 0.0 0.0 0.0 6 0.06528844370616611 0.0 0.0 0.0 0.0 7 0.10979785309900751 0.0 0.0 0.0 0.0 8 0.28568180957071954 0.0 0.0 0.0 0.0 9 0.9168300207192098 0.0 0.0 0.0 0.0 10-11 2.1055223972863617 0.0 0.0 0.0 0.0 12-13 3.227490542747579 0.0 0.0 0.0 0.0 14-15 4.277788967170322 0.0 0.0 0.0 0.0 16-17 5.208797388462251 0.0 0.0 0.0 0.0 18-19 6.1671256533331205 0.0 0.0 0.0 0.0 20-21 7.417517005107015 0.0 0.0 0.0 0.0 22-23 8.941784803386863 0.0 0.0 0.0 0.0 24-25 10.72776473825795 0.0 0.0 0.0 0.0 26-27 12.705960711273125 0.0 0.0 0.0 0.0 28-29 14.95446360378649 0.0 0.0 0.0 0.0 30-31 17.43390891125438 0.0 0.0 0.0 0.0 32-33 20.28482432542919 0.0 0.0 0.0 0.0 34-35 23.14383598521937 0.0 0.0 0.0 0.0 36-37 25.173062235401332 1.994149166345941E-5 0.0 0.0 0.0 38-39 26.616307753052546 3.988298332691882E-5 0.0 0.0 0.0 40-41 27.97593859615887 3.988298332691882E-5 0.0 0.0 0.0 42-43 29.00972546548427 3.988298332691882E-5 0.0 0.0 0.0 44-45 29.781780256726762 3.988298332691882E-5 0.0 0.0 0.0 46-47 30.38760277346266 3.988298332691882E-5 0.0 0.0 0.0 48-49 30.92223416496001 3.988298332691882E-5 0.0 0.0 0.0 50-51 31.444023235826087 3.988298332691882E-5 1.994149166345941E-5 0.0 0.0 52-53 32.00090933201986 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 54-55 32.496914054165075 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 56-57 32.986019020194746 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 58-59 33.447784201153816 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 60-61 33.89319735894884 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 62-63 34.32634649937084 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 64-65 34.74416063270365 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 66-67 35.117784420510226 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 68-69 35.432780222826224 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 70-71 35.72330781487116 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 72-73 35.99943764993509 3.988298332691882E-5 3.988298332691882E-5 0.0 0.0 74-75 36.26246592497613 3.988298332691882E-5 3.988298332691882E-5 1.994149166345941E-5 0.0 76-77 36.50872340552818 3.988298332691882E-5 3.988298332691882E-5 3.988298332691882E-5 0.0 78-79 36.7291367128844 3.988298332691882E-5 3.988298332691882E-5 3.988298332691882E-5 0.0 80-81 36.93551121010954 3.988298332691882E-5 3.988298332691882E-5 3.988298332691882E-5 0.0 82-83 37.14001120711831 3.988298332691882E-5 3.988298332691882E-5 3.988298332691882E-5 0.0 84-85 37.34132056546094 3.988298332691882E-5 3.988298332691882E-5 3.988298332691882E-5 0.0 86-87 37.53630847094624 3.988298332691882E-5 3.988298332691882E-5 5.9824474990378235E-5 0.0 88 37.68523153068896 3.988298332691882E-5 3.988298332691882E-5 7.976596665383764E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTTTC 460 0.0 83.85253 1 CATTTTC 675 0.0 78.05027 1 CCTTTTC 1265 0.0 75.11401 1 GCATTTT 420 0.0 75.038765 1 CCATTTT 480 0.0 74.478775 1 AACTTTT 660 0.0 74.12241 1 ACTTTTC 990 0.0 72.22183 1 GACTTTT 705 0.0 72.06009 1 GCCTTTT 920 0.0 71.07013 1 ACTTACG 20 0.0021568167 70.55319 2 TATTTTC 1530 0.0 67.94554 1 CACTTTT 1015 0.0 67.66233 1 AATTTCT 425 0.0 67.515114 1 ACATTTT 490 0.0 67.19889 1 AATTGTC 35 3.6235542E-6 67.19352 2 GGATTTT 465 0.0 66.76534 1 ACCTTTT 1080 0.0 66.20335 1 CGTTTTT 2275 0.0 65.751526 1 ACTTTCT 970 0.0 64.982025 1 AGCTTTT 390 0.0 63.925095 1 >>END_MODULE