FastQCFastQC Report
Thu 26 May 2016
SRR1248469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1248469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86457361
Sequences flagged as poor quality0
Sequence length100
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9669681.1184333974755487No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1501260.17364166366354855No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1314490.1520391074624635No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA896620.10370661209518066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGCT75850.051.2393237
GCTTGCT108600.051.1927457
CCGTTTG44250.048.693441
CGCTTGC64600.047.4295126
TGCTTGC37700.047.3676
TGCCTGC35100.047.126936
CGTTGCT41450.045.8047337
CGCTGAA30650.045.080819
CTGCTGA576600.044.8538559
CCGTTGC26000.044.8241356
CCTGCTG425350.044.7932138
CGCCTGC43400.044.3943526
GCTGCTG81800.043.894998
CCCTGCT232900.043.807037
GCCTTGC37650.043.560626
CCGTGCT49750.043.547317
GGCCATC51750.042.8653688
CGTGCTG64650.041.5817158
CCGCTTG37050.041.5069965
CCCGTGC31350.041.3718726
Produced by FastQC (version 0.11.5)