##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1248469_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 86457361 Sequences flagged as poor quality 0 Sequence length 100 %GC 25 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.752737560425885 34.0 31.0 34.0 31.0 34.0 2 32.9577112815183 34.0 31.0 34.0 31.0 34.0 3 33.03095806960844 34.0 31.0 34.0 31.0 34.0 4 36.394034372619814 37.0 37.0 37.0 35.0 37.0 5 36.16595680037007 37.0 35.0 37.0 35.0 37.0 6 36.10043553145232 37.0 35.0 37.0 35.0 37.0 7 35.975465779021405 37.0 35.0 37.0 35.0 37.0 8 35.96738878023353 37.0 35.0 37.0 35.0 37.0 9 37.72170690012155 39.0 37.0 39.0 35.0 39.0 10-11 37.74607479633805 39.0 37.0 39.0 35.0 39.0 12-13 37.604745147148314 39.0 37.0 39.0 35.0 39.0 14-15 38.6575067043742 40.0 38.0 41.0 34.5 41.0 16-17 38.95390769560963 40.0 38.0 41.0 36.0 41.0 18-19 39.01096324233167 40.0 38.5 41.0 35.0 41.0 20-21 38.76578566283095 40.0 38.0 41.0 34.5 41.0 22-23 38.4513732208412 40.0 37.5 41.0 34.0 41.0 24-25 38.30535324227627 40.0 37.0 41.0 34.0 41.0 26-27 38.421947091352926 40.0 37.5 41.0 34.0 41.0 28-29 38.45724548543646 40.0 38.0 41.0 34.0 41.0 30-31 38.31409296080643 40.0 37.5 41.0 34.0 41.0 32-33 38.24477564726964 40.0 37.0 41.0 34.0 41.0 34-35 38.17347116343281 40.0 37.0 41.0 34.0 41.0 36-37 38.12478043367528 40.0 37.0 41.0 33.0 41.0 38-39 38.05191001608296 40.0 37.0 41.0 33.0 41.0 40-41 37.95837921192158 40.0 37.0 41.0 33.0 41.0 42-43 37.887127037106765 40.0 37.0 41.0 33.0 41.0 44-45 37.88674604005088 40.0 37.0 41.0 33.0 41.0 46-47 37.86000947912348 40.0 37.0 41.0 33.0 41.0 48-49 37.83120738556894 40.0 37.0 41.0 33.0 41.0 50-51 37.69903432513976 40.0 36.0 41.0 33.0 41.0 52-53 37.510504091143844 40.0 36.0 41.0 32.0 41.0 54-55 37.31473372753073 39.0 35.0 41.0 32.0 41.0 56-57 37.11211238566489 39.0 35.0 41.0 31.0 41.0 58-59 36.886706523461896 39.0 35.0 41.0 31.0 41.0 60-61 36.65248877998948 38.5 35.0 41.0 31.0 41.0 62-63 36.41260406965232 38.0 35.0 41.0 30.5 41.0 64-65 36.13904794642066 37.0 35.0 41.0 30.0 41.0 66-67 35.902541062987105 37.0 35.0 40.0 30.0 41.0 68-69 35.67235745259447 36.5 34.5 40.0 30.0 41.0 70-71 35.39155937803838 36.0 34.0 39.0 29.5 41.0 72-73 35.0797457835892 36.0 34.0 39.0 29.0 41.0 74-75 34.76652733478645 35.0 34.0 39.0 29.0 41.0 76-77 34.588442110787994 35.0 34.0 37.5 29.0 40.0 78-79 34.350606711208776 35.0 34.0 37.0 29.0 39.0 80-81 34.09310048799662 35.0 34.0 37.0 29.0 39.0 82-83 33.844257795469844 35.0 34.0 36.0 29.0 39.0 84-85 33.63790270558917 35.0 34.0 36.0 29.0 37.5 86-87 33.489873748286165 35.0 34.0 36.0 29.0 37.0 88-89 33.36845766088095 35.0 34.0 35.5 29.0 37.0 90-91 33.215539871729376 35.0 34.0 35.0 29.0 36.0 92-93 33.108842935883736 35.0 34.0 35.0 29.0 36.0 94-95 33.05185741790106 35.0 34.0 35.0 29.0 36.0 96-97 33.027396851726714 35.0 34.0 35.0 29.0 36.0 98-99 32.9829383642649 35.0 34.0 35.0 29.0 36.0 100 31.76889357055439 34.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06295977606134073 1101 2 0.07634252681546627 1101 3 0.046354150013627304 1101 4 0.004050283164559687 1101 5 0.004605456782101669 1101 6 0.03820814507115955 1101 7 0.03695514637523445 1101 8 -0.012308417439129471 1101 9 0.10921293783706432 1101 10-11 0.12044361413771298 1101 12-13 0.2583143118896629 1101 14-15 0.2805159959456063 1101 16-17 0.2229437473651572 1101 18-19 0.08978137194774405 1101 20-21 0.14750084944205355 1101 22-23 0.2023483675008535 1101 24-25 0.08989358963633975 1101 26-27 -0.019657485825341325 1101 28-29 -0.05819293964842842 1101 30-31 0.03361185161598712 1101 32-33 0.127349897354037 1101 34-35 0.07955150249865994 1101 36-37 0.05839494686588864 1101 38-39 7.221000000612321E-4 1101 40-41 -0.002066986606841681 1101 42-43 0.008501106206054487 1101 44-45 0.038384628686650046 1101 46-47 -0.004773919197624821 1101 48-49 0.02089476054569417 1101 50-51 -0.10519678812728017 1101 52-53 0.036189276182980734 1101 54-55 0.05237882820224371 1101 56-57 0.14050660274357085 1101 58-59 0.06555244932200566 1101 60-61 -0.010911235829702548 1101 62-63 -0.005415488516078426 1101 64-65 0.10745570229864398 1101 66-67 0.047900279747672414 1101 68-69 0.06983019358717968 1101 70-71 0.10321075298634241 1101 72-73 0.09201751451596607 1101 74-75 0.15973827517241546 1101 76-77 0.1596565814596289 1101 78-79 0.10934946349098595 1101 80-81 0.07563909033491001 1101 82-83 0.05922757866737527 1101 84-85 0.03048025515629149 1101 86-87 0.032938130617466754 1101 88-89 -0.021200455395238293 1101 90-91 -0.07828313403454956 1101 92-93 0.04360458936270817 1101 94-95 0.034025673082680896 1101 96-97 0.03876129870091205 1101 98-99 -0.07033309639136576 1101 100 -0.10246917847382875 1102 1 0.12077269989994477 1102 2 0.11059454247554612 1102 3 0.08860674387607759 1102 4 0.03170738164858733 1102 5 0.05378861000368573 1102 6 0.0849075335183187 1102 7 0.07103994254710244 1102 8 0.09516779831210442 1102 9 0.15239843858486068 1102 10-11 0.11978469648247625 1102 12-13 0.24135801382344368 1102 14-15 0.5019984439789482 1102 16-17 0.3094218994437057 1102 18-19 0.31452113846828667 1102 20-21 0.4446231293756995 1102 22-23 0.45877300574401403 1102 24-25 0.3775256099777735 1102 26-27 0.35534940681844773 1102 28-29 0.3780248375436983 1102 30-31 0.4098448673761581 1102 32-33 0.3667707419340971 1102 34-35 0.38697112029404224 1102 36-37 0.3278631686859299 1102 38-39 0.26499346953298186 1102 40-41 0.29377613398771274 1102 42-43 0.2951767593053347 1102 44-45 0.1819617506664173 1102 46-47 0.21485370335988563 1102 48-49 0.24916936392145317 1102 50-51 0.2517885922245142 1102 52-53 0.26266311693465383 1102 54-55 0.24823175907587824 1102 56-57 0.21815416611666194 1102 58-59 0.2225625613250699 1102 60-61 0.22673148419794842 1102 62-63 0.22879138122058862 1102 64-65 0.2252453886996264 1102 66-67 0.19931443702262897 1102 68-69 0.2268526037304852 1102 70-71 0.22924535586414407 1102 72-73 0.24125064346932135 1102 74-75 0.21964397007201342 1102 76-77 0.17942492559500778 1102 78-79 0.19570487504998368 1102 80-81 0.2133246370347308 1102 82-83 0.19785165706257857 1102 84-85 0.2586190171442979 1102 86-87 0.4000918464054948 1102 88-89 0.3619986701750051 1102 90-91 0.3076275755632025 1102 92-93 0.30681392153785225 1102 94-95 0.31705969366962705 1102 96-97 0.19481745542840656 1102 98-99 0.15652117480458116 1102 100 0.18658279248201737 1103 1 0.07702663954626843 1103 2 0.06501498255904181 1103 3 0.036469941554543084 1103 4 1.0222576687368701E-4 1103 5 0.03192953627743833 1103 6 0.06635452271645192 1103 7 0.08461084641785988 1103 8 -0.04070499699996333 1103 9 0.031850239849703144 1103 10-11 0.061744655067883514 1103 12-13 0.16972411334253934 1103 14-15 0.2184929191514442 1103 16-17 0.2393600880582838 1103 18-19 0.20516514369469974 1103 20-21 0.20700429400690723 1103 22-23 0.1381072434113051 1103 24-25 0.15808886589712756 1103 26-27 0.10174410144396973 1103 28-29 0.07759225547664528 1103 30-31 0.10555357813926491 1103 32-33 0.20267546228279087 1103 34-35 0.13195122863573516 1103 36-37 0.11186587256700875 1103 38-39 0.0868143974627884 1103 40-41 0.05241933630988882 1103 42-43 0.08996431404931116 1103 44-45 0.13408590822349709 1103 46-47 0.2330040902903292 1103 48-49 0.22938841532252496 1103 50-51 0.2234708970894772 1103 52-53 0.2207877623343677 1103 54-55 0.22608785814204424 1103 56-57 0.22084099160534265 1103 58-59 0.20677439587398538 1103 60-61 0.19341262896163158 1103 62-63 0.18651062705767174 1103 64-65 0.20073756370958762 1103 66-67 0.19726797587933476 1103 68-69 0.18876787800034123 1103 70-71 0.17777726755910095 1103 72-73 0.18412016302534795 1103 74-75 0.1567984722192719 1103 76-77 0.12305249100349869 1103 78-79 0.09286398264242024 1103 80-81 0.1093357091154985 1103 82-83 0.0835217970961537 1103 84-85 0.1303915718879054 1103 86-87 0.2423908334186038 1103 88-89 0.13031547332676752 1103 90-91 0.15719925888474506 1103 92-93 0.09836054024864893 1103 94-95 0.1888315620604999 1103 96-97 0.28953032756280095 1103 98-99 0.20141510963395604 1103 100 0.3126644146499018 1104 1 0.08572362123420874 1104 2 0.07466598932785473 1104 3 0.04321051246471086 1104 4 0.011721055444184003 1104 5 0.019183867294174206 1104 6 0.03601395855309164 1104 7 0.06406061940489138 1104 8 0.08265680240176465 1104 9 0.12523066639908365 1104 10-11 0.10249099215150181 1104 12-13 0.18812339541719325 1104 14-15 0.2922586408917738 1104 16-17 0.2391908462619199 1104 18-19 0.2593504459033298 1104 20-21 0.3904917826695211 1104 22-23 0.3072321731641736 1104 24-25 0.41846760291810625 1104 26-27 0.2683825747540354 1104 28-29 0.2008619393052129 1104 30-31 0.21904411527728485 1104 32-33 0.32609296117445297 1104 34-35 0.2785126906517732 1104 36-37 0.2985033721365724 1104 38-39 0.3391722016149288 1104 40-41 0.11218440777063421 1104 42-43 0.2217036687092886 1104 44-45 0.26230629517052506 1104 46-47 0.23644492435344233 1104 48-49 0.21980827812723192 1104 50-51 0.21879173731338852 1104 52-53 0.22037970761944337 1104 54-55 0.2254472812363133 1104 56-57 0.19149758286023655 1104 58-59 0.19272172991302483 1104 60-61 0.1887693668028092 1104 62-63 0.19162681554388428 1104 64-65 0.17964206411488703 1104 66-67 0.18536380199655156 1104 68-69 0.20364850287547398 1104 70-71 0.20943725522558054 1104 72-73 0.20801713635425045 1104 74-75 0.18569789999789066 1104 76-77 0.17234728778219477 1104 78-79 0.18576732163602827 1104 80-81 0.22311164130393024 1104 82-83 0.1332304553651369 1104 84-85 0.14105662504751848 1104 86-87 0.16075303167549038 1104 88-89 0.1727564552082086 1104 90-91 0.1929716221981579 1104 92-93 0.1893217531710718 1104 94-95 0.1657856166597469 1104 96-97 0.2655478714758601 1104 98-99 0.296067162937554 1104 100 0.2727899294919496 1105 1 0.07794803304891218 1105 2 0.07622123594022412 1105 3 0.05313168515660038 1105 4 0.031104445466709763 1105 5 0.03876907922323625 1105 6 0.06107035993093746 1105 7 0.07991977204016365 1105 8 0.0731224776324737 1105 9 0.10175677687325191 1105 10-11 0.12154416116144517 1105 12-13 0.2536710965551947 1105 14-15 0.20991090237886567 1105 16-17 0.14195100682407258 1105 18-19 0.2280996266395121 1105 20-21 0.2728481333104895 1105 22-23 0.19683136715698168 1105 24-25 0.18857746147492094 1105 26-27 0.2183651892787779 1105 28-29 0.22793121740051703 1105 30-31 0.2363896495114517 1105 32-33 0.2594550048994293 1105 34-35 0.10332722841284436 1105 36-37 0.022553663705153326 1105 38-39 0.1300116433199534 1105 40-41 0.21858562673159554 1105 42-43 0.11593319369638522 1105 44-45 0.14848041725582561 1105 46-47 0.14573114452134206 1105 48-49 0.12445062722196809 1105 50-51 0.1316719195233631 1105 52-53 0.1745265924950985 1105 54-55 0.1456704291163078 1105 56-57 0.08298305813987383 1105 58-59 0.06353951564008042 1105 60-61 0.1406389802432173 1105 62-63 0.15802140892240857 1105 64-65 0.12972672918517247 1105 66-67 0.12307966146232019 1105 68-69 0.1154972213853469 1105 70-71 0.11590997748997722 1105 72-73 0.13567317642198873 1105 74-75 0.13527863713575528 1105 76-77 0.10606384248072231 1105 78-79 0.1980544764441845 1105 80-81 0.1642020907187316 1105 82-83 0.20149784524335956 1105 84-85 0.2344328387118324 1105 86-87 0.28802313351557274 1105 88-89 0.13888743493779998 1105 90-91 0.1333310324872201 1105 92-93 0.18188933241833638 1105 94-95 0.26585174094800834 1105 96-97 0.31409720369749294 1105 98-99 0.2508395035409876 1105 100 0.2636274028103287 1106 1 0.07427182252273923 1106 2 0.0556918448953283 1106 3 0.033930883086213726 1106 4 0.014548307471983435 1106 5 0.04080461727689766 1106 6 0.00945923561679507 1106 7 0.02838738252788886 1106 8 -0.020487301685427894 1106 9 0.043164337369944406 1106 10-11 0.11188861028844599 1106 12-13 0.11204005547760687 1106 14-15 -0.0040305053579103856 1106 16-17 0.09440577126542138 1106 18-19 0.14761247579030368 1106 20-21 0.07307596424821838 1106 22-23 0.025821559975788944 1106 24-25 0.09449757435357498 1106 26-27 0.2089966844215141 1106 28-29 0.2959277066431696 1106 30-31 0.02310907048769195 1106 32-33 -0.017009462379483864 1106 34-35 0.021486949861348137 1106 36-37 0.09506641918900982 1106 38-39 0.18525322093179852 1106 40-41 0.16041824665189353 1106 42-43 0.06050496994306087 1106 44-45 0.1510456543288754 1106 46-47 0.17407260806918856 1106 48-49 0.15457952648315398 1106 50-51 0.16303798312921458 1106 52-53 0.16737836320214683 1106 54-55 0.15308189677544703 1106 56-57 0.14942528366429997 1106 58-59 0.1509974468242845 1106 60-61 0.1593089618511172 1106 62-63 0.16486333211109638 1106 64-65 0.15346726670460242 1106 66-67 0.16309369329913892 1106 68-69 0.1443826996897215 1106 70-71 0.13592825788046525 1106 72-73 0.14257645183337075 1106 74-75 0.14848086551121753 1106 76-77 0.12486421536987535 1106 78-79 0.15002994715941753 1106 80-81 0.16948959954709153 1106 82-83 0.11957077685396911 1106 84-85 0.15300000698513117 1106 86-87 0.05602353631787338 1106 88-89 0.07418673618523286 1106 90-91 -0.024256346386266614 1106 92-93 0.1464774551770347 1106 94-95 0.14493830688891762 1106 96-97 0.09201787577648446 1106 98-99 0.1995128486201807 1106 100 0.265342628257514 1107 1 0.01895394632929026 1107 2 0.009582445071586676 1107 3 0.00966238292330246 1107 4 0.020832165978944772 1107 5 -0.008431301314843154 1107 6 -0.03575777495613863 1107 7 0.006598970133694593 1107 8 0.0037720998141281825 1107 9 -0.02122028848216928 1107 10-11 0.058330537268922455 1107 12-13 0.015390011197141007 1107 14-15 -0.14597370618504613 1107 16-17 0.048353183621308915 1107 18-19 0.13833222485099128 1107 20-21 0.12505180977463226 1107 22-23 0.18064618513539443 1107 24-25 0.14103987774468862 1107 26-27 0.14495691245146958 1107 28-29 0.1682350006860105 1107 30-31 0.09524877746609661 1107 32-33 0.08449354687888189 1107 34-35 0.1367061038065387 1107 36-37 0.11867975450876145 1107 38-39 0.039898248268322334 1107 40-41 0.04274479602452175 1107 42-43 -0.05738901248331274 1107 44-45 0.09165144152747473 1107 46-47 0.12463649297961865 1107 48-49 0.09905524623479067 1107 50-51 0.08905655316765149 1107 52-53 0.14345336054833524 1107 54-55 0.1250211528653793 1107 56-57 0.0911691846421121 1107 58-59 0.08593263823597397 1107 60-61 0.08333682676371978 1107 62-63 0.05231624295869608 1107 64-65 0.07003458846540411 1107 66-67 0.06319540910606491 1107 68-69 0.07185817292015173 1107 70-71 0.06905824618557688 1107 72-73 0.08878471768665719 1107 74-75 0.10300436831986559 1107 76-77 0.07412655794595935 1107 78-79 0.16999259243045373 1107 80-81 0.13615809192268102 1107 82-83 0.054432459829804714 1107 84-85 0.12567623357066537 1107 86-87 0.16369266652496606 1107 88-89 0.15046856622603144 1107 90-91 0.12974359398401702 1107 92-93 0.15535562957862226 1107 94-95 0.2530057759805686 1107 96-97 0.22862492374248689 1107 98-99 0.11162263404704476 1107 100 0.20563207489641044 1108 1 0.008317240098520529 1108 2 0.019192714067152394 1108 3 0.013004914507810383 1108 4 0.0211100280271026 1108 5 -0.015703967689205456 1108 6 0.012991193987815564 1108 7 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2214 24-25 -0.5463886150511144 2214 26-27 -0.31571956886165964 2214 28-29 -0.24569247653200676 2214 30-31 -0.16349205138740075 2214 32-33 -0.0756434719189869 2214 34-35 -0.08866201489588121 2214 36-37 -0.08770219251086075 2214 38-39 -0.12166088580285361 2214 40-41 -0.057502806679153196 2214 42-43 -0.14087252205467848 2214 44-45 -0.10698315408241399 2214 46-47 -0.11816230342596867 2214 48-49 -0.1356744557195242 2214 50-51 -0.1398686954266921 2214 52-53 -0.1470348384581115 2214 54-55 -0.1643631340476901 2214 56-57 -0.16025781102212022 2214 58-59 -0.14684039203589094 2214 60-61 -0.1646611148287107 2214 62-63 -0.1431822250387711 2214 64-65 -0.15781057110993402 2214 66-67 -0.15930560954814865 2214 68-69 -0.155700697233506 2214 70-71 -0.16639731297858873 2214 72-73 -0.16686018967747884 2214 74-75 -0.14345579816442466 2214 76-77 -0.18577219436298975 2214 78-79 -0.16491163609452286 2214 80-81 -0.20679700517796107 2214 82-83 -0.21472049167137186 2214 84-85 -0.20342358730801635 2214 86-87 -0.1643190483826089 2214 88-89 -0.10795736167168712 2214 90-91 -0.17570694060234615 2214 92-93 -0.020496343760811442 2214 94-95 -0.1153224400103312 2214 96-97 -0.24665428376493992 2214 98-99 -0.11554257914117017 2214 100 -0.14741664846537716 2215 1 -0.16192450412323467 2215 2 -0.13767878528710042 2215 3 -0.11977306699406398 2215 4 -0.03676468276784561 2215 5 -0.06778339828190383 2215 6 -0.13036645648784884 2215 7 -0.127648134309311 2215 8 -0.12204210856884856 2215 9 -0.14918007142908607 2215 10-11 -0.19529488630799818 2215 12-13 -0.3656596042682807 2215 14-15 -0.645801075413182 2215 16-17 -0.4284024936670221 2215 18-19 -0.537013119405799 2215 20-21 -0.5051056350268084 2215 22-23 -0.5251346357509945 2215 24-25 -0.5278692778674099 2215 26-27 -0.5048941780462783 2215 28-29 -0.3253820434550292 2215 30-31 -0.5209410164924506 2215 32-33 -0.47027409003118237 2215 34-35 -0.42036758066426927 2215 36-37 -0.5832613807942195 2215 38-39 -0.49661364497072213 2215 40-41 -0.39525581736925375 2215 42-43 -0.39403420482032914 2215 44-45 -0.34643477344850737 2215 46-47 -0.29609576010907546 2215 48-49 -0.3256381341054819 2215 50-51 -0.3011603707653876 2215 52-53 -0.31571029928576877 2215 54-55 -0.30489313736069334 2215 56-57 -0.2833294078688837 2215 58-59 -0.29916615925090184 2215 60-61 -0.26073412895768655 2215 62-63 -0.2356588636412198 2215 64-65 -0.24160904143997897 2215 66-67 -0.23960792800139075 2215 68-69 -0.22166826388424 2215 70-71 -0.21963517050761538 2215 72-73 -0.23487247461820715 2215 74-75 -0.2166832268117247 2215 76-77 -0.19852924855526766 2215 78-79 -0.21373361636300103 2215 80-81 -0.2516679059656681 2215 82-83 -0.27372865522545453 2215 84-85 -0.2394392210015326 2215 86-87 -0.13381391256053377 2215 88-89 -0.19411498086302714 2215 90-91 -0.21298693175782546 2215 92-93 -0.17131191751521158 2215 94-95 -0.1542969567617547 2215 96-97 -0.033465010468447076 2215 98-99 -0.004193370030733945 2215 100 -0.16117378026435603 2216 1 -0.16458631368292487 2216 2 -0.14091977841950154 2216 3 -0.1341158857492104 2216 4 -0.04103068527667375 2216 5 -0.06792173775080812 2216 6 -0.09454768413325354 2216 7 -0.12147352521738952 2216 8 -0.12053035694374614 2216 9 -0.16653146954417508 2216 10-11 -0.1977023118464345 2216 12-13 -0.4791345611539839 2216 14-15 -1.0137336582053962 2216 16-17 -0.6530440663804882 2216 18-19 -0.5840171018260634 2216 20-21 -0.7854449526264133 2216 22-23 -0.8864343220560755 2216 24-25 -0.969139720742703 2216 26-27 -0.6022145862147497 2216 28-29 -0.5208017460805365 2216 30-31 -0.671757216066311 2216 32-33 -0.8581677145893423 2216 34-35 -0.6985160293175667 2216 36-37 -0.790760676217694 2216 38-39 -0.6186586532203506 2216 40-41 -0.3811124180428749 2216 42-43 -0.4238812008245887 2216 44-45 -0.3342151324858591 2216 46-47 -0.23123937389416227 2216 48-49 -0.20694625462369487 2216 50-51 -0.25546709201147877 2216 52-53 -0.2515588428888833 2216 54-55 -0.24347953056318516 2216 56-57 -0.26992541966481554 2216 58-59 -0.23272288751910253 2216 60-61 -0.22111937224477884 2216 62-63 -0.24477650094334535 2216 64-65 -0.24665590219733957 2216 66-67 -0.22352186875878743 2216 68-69 -0.20466638486320932 2216 70-71 -0.18264627414835388 2216 72-73 -0.18934760692537367 2216 74-75 -0.19680943005398888 2216 76-77 -0.2375000768015596 2216 78-79 -0.2525386960019276 2216 80-81 -0.244954666070619 2216 82-83 -0.2305514833227278 2216 84-85 -0.19587303303576675 2216 86-87 -0.23096689397933545 2216 88-89 -0.23064734620703575 2216 90-91 -0.2553307796044919 2216 92-93 -0.29899744665855366 2216 94-95 -0.2534662902649174 2216 96-97 -0.14587992619173917 2216 98-99 -0.21518271021039226 2216 100 -0.25349309374327333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 35.0 8 216.0 9 582.0 10 1391.0 11 2545.0 12 4348.0 13 6472.0 14 9556.0 15 14871.0 16 23380.0 17 36908.0 18 55679.0 19 81079.0 20 115395.0 21 161413.0 22 218263.0 23 285642.0 24 363290.0 25 453946.0 26 558276.0 27 686949.0 28 853530.0 29 1076302.0 30 1382327.0 31 1820456.0 32 2481979.0 33 3582348.0 34 5485229.0 35 9320269.0 36 1.0023988E7 37 1.5525956E7 38 2.0073877E7 39 1.1407183E7 40 343680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 11.943041590214733 29.99711330882846 29.406987620240532 28.652857480716275 2 9.265857149039087 33.135094088405104 31.264826286303403 26.334222476252407 3 8.08880984978008 29.760682485924484 30.639486315812558 31.511021348482878 4 7.5012252588840385 29.16119268638198 32.87050700315738 30.467075051576597 5 6.088290865263666 29.386198508706535 34.422384322969776 30.103126303060023 6 5.70064374290669 24.190193404166802 31.58894280590933 38.520220047017176 7 6.869461352168729 28.136065823244362 29.279195787620676 35.71527703696623 8 5.738250805603971 27.931908214034685 28.459697790523663 37.87014318983768 9 6.252569980709913 28.81540994525614 27.916223350837644 37.0157967231963 10-11 4.703904274848269 47.727793241341246 31.384570019434204 16.183732464376284 12-13 4.3200835171568075 47.49980632784966 31.057564452486737 17.122545702506795 14-15 4.554031506187879 45.470670735879054 31.112758711208393 18.86253904672467 16-17 5.246782791469296 44.87560019446274 31.247536012737665 18.630081001330304 18-19 5.281920528999333 43.530953102671155 31.751315923385746 19.435810444943762 20-21 4.406653708573938 42.96537506294446 33.126728717276514 19.501242511205085 22-23 4.328690416539547 42.58139454430028 33.55978214509693 19.530132894063236 24-25 4.386299674366075 42.447046932416605 33.27456350019419 19.892089893023133 26-27 5.198849032473214 42.49971673894045 32.50538577704234 19.79604845154399 28-29 4.704580330644142 42.94647508382774 33.24234416546672 19.106600420061397 30-31 4.627312288705547 42.78483883324026 33.42211748092708 19.16573139712712 32-33 4.807221210631705 42.79233766555372 33.33034727558587 19.070093848228698 34-35 4.863255217901523 42.606259952749376 33.30824386697586 19.22224096237325 36-37 4.97407353604365 42.692735553502864 32.96924500311087 19.363945907342615 38-39 5.0620547329990835 43.0615254421106 32.918174660198424 18.958245164691895 40-41 5.080433871313382 43.28237430536802 32.98471113496878 18.652480688349822 42-43 5.10070258610776 43.169803327695874 33.208239678251026 18.521254407945342 44-45 5.285289326591103 43.15420528723769 32.92580923557944 18.634696150591758 46-47 5.517330887436765 43.2704520551389 32.70910547972904 18.503111577695297 48-49 5.47359444051334 43.3216330148871 32.884040842825904 18.320731701773656 50-51 5.5374242864161625 43.114604061666846 33.093849522928984 18.254122128988005 52-53 5.516592585733265 42.95717968254261 33.19928368106238 18.32694405066175 54-55 5.5593150223051735 42.794270447756425 33.21217583288546 18.43423869705295 56-57 5.682987977589554 42.837827538222264 32.9784274568164 18.50075702737179 58-59 5.742222130718038 42.97876519145761 33.17986481664099 18.099147861183358 60-61 5.743261023358988 43.051023826519916 33.14931563260657 18.056399517514528 62-63 5.836789754906963 42.83420610413036 33.02281727786551 18.30618686309717 64-65 5.980247303639074 42.74038158532274 32.795398994424545 18.483972116613646 66-67 6.03525690976179 42.8048262933705 32.71406613774135 18.44585065912636 68-69 6.100022706569274 43.083438062706946 32.701308472199045 18.115230758524735 70-71 6.114255557719371 43.25517580856996 32.75535960437191 17.87520902933875 72-73 6.1881769673723905 43.024603191392806 32.80249960440037 17.98472023683443 74-75 6.273275129766572 42.82271564644948 32.64079810719178 18.263211116592167 76-77 6.416296308702328 42.88766567042204 32.54249442262546 18.15354359825017 78-79 6.461826309965672 42.98589509342067 32.61361111866461 17.938667477949043 80-81 6.438573719001002 42.82585770781171 32.791727437053325 17.94384113613397 82-83 6.4974318218937555 42.57088016408191 32.814911287841696 18.11677672618263 84-85 6.650967826225912 42.553756701170684 32.608796336058134 18.18647913654527 86-87 6.745834405085523 42.703438036052006 32.44573668256866 18.104990876293815 88-89 6.768757520580157 42.96181266385195 32.406166015176076 17.86326380039182 90-91 6.760269150708862 42.82653758579686 32.5553997480464 17.857793515447874 92-93 6.832724362864527 42.50020297794717 32.623951601336245 18.043121057852066 94-95 6.958070927008748 42.383971215591465 32.547380205139504 18.110577652260286 96-97 7.0625710287540775 42.56186320503947 32.334743428478205 18.040822337728244 98-99 7.134409460737749 42.59111650634621 32.37806859217155 17.896405440744495 100 7.102928157364541 42.33695322652956 32.51824826646853 18.041870349637374 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 495075.0 1 925762.0 2 1099959.5 3 525209.5 4 139295.5 5 67505.0 6 68928.5 7 86424.0 8 119888.0 9 177469.5 10 272294.5 11 419224.5 12 630757.0 13 911075.5 14 1260256.5 15 1667521.0 16 2112243.0 17 2575599.0 18 3038485.5 19 3470275.5 20 3837307.5 21 4122455.5 22 4313568.5 23 4415030.0 24 4437077.0 25 4374114.0 26 4229351.0 27 4018278.0 28 3752067.0 29 3441017.5 30 3096406.5 31 2730276.5 32 2369157.5 33 2036467.5 34 1744782.5 35 1495590.0 36 1280816.5 37 1098744.0 38 945639.5 39 816456.5 40 707125.0 41 614817.0 42 538103.0 43 475214.5 44 424977.0 45 385112.5 46 351361.0 47 321632.0 48 300023.5 49 296621.5 50 316807.0 51 357906.0 52 409085.5 53 452250.0 54 468503.5 55 445020.0 56 382718.0 57 297080.5 58 209437.0 59 134984.5 60 80340.0 61 44857.0 62 24380.5 63 13741.0 64 8312.5 65 5556.5 66 3916.0 67 2895.5 68 2307.5 69 1886.5 70 1522.0 71 1229.0 72 1059.0 73 912.0 74 769.0 75 654.5 76 581.5 77 484.0 78 385.0 79 339.5 80 312.5 81 281.0 82 231.0 83 204.0 84 179.5 85 152.0 86 140.5 87 126.0 88 104.5 89 86.5 90 63.0 91 46.0 92 38.0 93 36.0 94 30.5 95 20.5 96 20.0 97 18.0 98 16.0 99 14.0 100 12.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006290962316094752 2 0.008607711262433745 3 0.0065280734164439745 4 0.05442104576844532 5 0.0025573299652299126 6 2.3132790277972977E-5 7 0.0 8 2.058818334739595E-4 9 0.0 10-11 0.0 12-13 0.0037804762511777336 14-15 0.001903828639877176 16-17 8.032861424026117E-4 18-19 4.5108941042047306E-4 20-21 2.2323142618243922E-4 22-23 0.0 24-25 3.5451001100993585E-4 26-27 1.5036313680682435E-5 28-29 0.0 30-31 1.2723034652885136E-4 32-33 2.313279027797298E-6 34-35 1.0988075382037166E-4 36-37 5.147045836848987E-5 38-39 2.041468742031115E-4 40-41 1.8332736295293583E-4 42-43 3.1402762802348313E-4 44-45 3.5393169125298653E-4 46-47 2.0299023468921287E-4 48-49 1.9662871736277033E-4 50-51 4.262216608716521E-4 52-53 3.9557071375333794E-4 54-55 7.402492888951353E-5 56-57 5.175961824696453E-4 58-59 0.001253218913309186 60-61 0.0038851521271855614 62-63 5.725365593798312E-5 64-65 0.0 66-67 4.510894104204731E-5 68-69 2.891598784746622E-6 70-71 0.0 72-73 0.0 74-75 2.313279027797298E-6 76-77 1.734959270847973E-5 78-79 0.0 80-81 1.064108352786757E-4 82-83 0.0011942302981003548 84-85 0.0059173677531054875 86-87 0.0027331391713425073 88-89 0.0014631489850817907 90-91 0.0011173137704260947 92-93 0.0010693132305993008 94-95 0.0 96-97 0.007468421341243576 98-99 2.313279027797298E-6 100 0.11579927821299103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8.6457361E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.53495050114027 #Duplication Level Percentage of deduplicated Percentage of total 1 97.77430104469673 90.47540107454599 2 1.7980737159180873 3.3276932459976307 3 0.18221883369874295 0.5058483227006607 4 0.07361222963937869 0.2724681610383391 5 0.047811026044336714 0.22120954642107132 6 0.029200344002895857 0.16212314321545415 7 0.014846339089576933 0.09616636769440018 8 0.011422756231610714 0.08456033459829512 9 0.010509958799021533 0.08752846655128331 >10 0.052349160360185824 0.8974740186506899 >50 0.0035198736664472704 0.22303331166605211 >100 0.001848306803736333 0.3169180200349153 >500 8.314781142504167E-5 0.048989931192947284 >1k 9.817667253165179E-5 0.2202494943375878 >5k 3.379359317076047E-5 0.21868384946601535 >10k+ 7.129297241052575E-5 2.841652711888651 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125878 0.1455954687305341 No Hit TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89102 0.10305889396739741 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.1976150764074328E-4 0.0 0.0 6.939837083391893E-6 0.0 2 5.459338505601622E-4 0.0 0.0 2.7759348333567573E-5 0.0 3 8.963956232714528E-4 0.0 0.0 6.824173132002027E-5 0.0 4 0.0013902806957061759 0.0 0.0 8.212140548680407E-5 0.0 5 0.002450919129951237 0.0 0.0 8.790460305629731E-5 0.0 6 0.004853259400318731 0.0 0.0 9.484444013968921E-5 0.0 7 0.008920003931186379 0.0 0.0 9.71577191674865E-5 0.0 8 0.029160038784898836 0.0 0.0 9.831435868138515E-5 0.0 9 0.10166629999266344 0.0 0.0 9.831435868138515E-5 0.0 10-11 0.23965686391931393 0.0 0.0 9.889267843833448E-5 0.0 12-13 0.36075933430353024 0.0 0.0 1.0641083527867569E-4 0.0 14-15 0.48040386058047735 0.0 0.0 1.2376042798715542E-4 0.0 16-17 0.5517725668263227 0.0 0.0 1.3821842191088853E-4 0.0 18-19 0.6108207489701195 0.0 0.0 1.6771272951530408E-4 0.0 20-21 0.6804562309043877 0.0 0.0 1.7580920611259463E-4 0.0 22-23 0.782579981824798 0.0 0.0 1.7870080489734125E-4 0.0 24-25 0.9162163763013771 0.0 0.0 1.8159240368208787E-4 0.0 26-27 1.0651186774021475 0.0 0.0 1.8332736295293583E-4 0.0 28-29 1.2227825228207 0.0 0.0 1.867972814946318E-4 0.0 30-31 1.3871496725420522 0.0 0.0 1.8853224076547975E-4 0.0 32-33 1.5694453130485906 0.0 0.0 1.9026720003632774E-4 0.0 34-35 1.7567439977724972 0.0 0.0 1.966287173627703E-4 0.0 36-37 1.9267347288104248 0.0 0.0 2.035685544461622E-4 0.0 38-39 2.100633744765816 0.0 0.0 2.2091814715464195E-4 0.0 40-41 2.330234784751295 0.0 0.0 2.3711110034922302E-4 0.0 42-43 2.5388312511643747 0.0 0.0 2.654487684397399E-4 0.0 44-45 2.7279510648029146 0.0 0.0 2.7759348333567576E-4 0.0 46-47 2.9003302564370426 0.0 0.0 2.8742491920381424E-4 0.0 48-49 3.062783167762893 0.0 0.0 3.221241046207737E-4 0.0 50-51 3.2320168782389738 0.0 0.0 3.921007952116419E-4 0.0 52-53 3.415336723035069 0.0 0.0 4.539810092052197E-4 0.0 54-55 3.6090911912058012 0.0 0.0 5.014032292750643E-4 0.0 56-57 3.8094651073145753 0.0 0.0 5.175961824696453E-4 0.0 58-59 4.012202037950245 0.0 0.0 5.274276183377839E-4 0.0 60-61 4.218933423147163 0.0 0.0 5.540303271574529E-4 0.0 62-63 4.434710307662526 0.0 0.0 5.736931988937298E-4 0.0 64-65 4.657525921939717 0.0 0.0 6.199587794496757E-4 0.0 66-67 4.8852231332853195 0.0 0.0 6.870438712557974E-4 0.0 68-69 5.119108366030279 0.0 0.0 7.321528122978447E-4 0.0 70-71 5.363223496955916 0.0 0.0 7.622254396592096E-4 0.0 72-73 5.617866939056814 0.0 0.0 7.842015904232838E-4 0.0 74-75 5.874277726334951 0.0 0.0 8.183224560832941E-4 0.0 76-77 6.133853079323113 1.1566395138986487E-6 0.0 9.131668962229832E-4 0.0 78-79 6.395131583995491 1.1566395138986487E-6 0.0 0.0010693132305993008 0.0 80-81 6.662072995727917 1.1566395138986487E-6 0.0 0.001228351163760365 0.0 82-83 6.937748770749549 1.1566395138986487E-6 0.0 0.0012728817850454631 0.0 84-85 7.221440635922256 1.1566395138986487E-6 0.0 0.0012937012962956386 0.0 86-87 7.510171979457018 1.1566395138986487E-6 5.783197569493244E-7 0.0013070026507054733 0.0 88 7.730250984644327 1.1566395138986487E-6 1.1566395138986487E-6 0.001313942487788865 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCAACC 1440 0.0 42.78773 1 GGCATCC 1100 0.0 39.764977 1 GTGCACC 650 0.0 39.07427 1 GGTAACC 1115 0.0 38.386368 1 GGACACC 1295 0.0 38.135548 1 GGTTACC 1080 0.0 37.888374 1 GGATACC 925 0.0 37.627075 1 GGCCACC 1950 0.0 37.38587 1 GACAGCC 1045 0.0 37.357025 1 CCCCTGG 2050 0.0 36.90972 6 GGCTACC 1240 0.0 36.792603 1 GGTCACC 990 0.0 35.156612 1 CCCAGTG 780 0.0 34.94884 7 GGCCTCC 2195 0.0 34.92718 1 GGCGACC 285 0.0 34.656517 1 GGACTTC 975 0.0 34.250282 1 GGTATCC 825 0.0 34.20643 1 GGCCTTC 1375 0.0 33.86437 1 GGCTTCC 1310 0.0 33.749474 1 CCCTTGG 1680 0.0 33.57155 7 >>END_MODULE