##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248467_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19066379
Sequences flagged as poor quality	0
Sequence length	100
%GC	15
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.283688738170998	31.0	26.0	33.0	16.0	34.0
2	29.042990753514342	31.0	28.0	33.0	18.0	34.0
3	29.65152580885967	31.0	30.0	34.0	23.0	34.0
4	33.449726348144026	35.0	33.0	37.0	28.0	37.0
5	33.211524275270094	35.0	33.0	37.0	26.0	37.0
6	32.87416472734545	35.0	32.0	37.0	25.0	37.0
7	33.291431529814865	35.0	33.0	37.0	27.0	37.0
8	33.071441724723925	35.0	32.0	37.0	26.0	37.0
9	34.19393535605266	37.0	33.0	39.0	25.0	39.0
10-11	35.26293605618561	37.0	34.0	39.0	29.5	39.0
12-13	35.3511506038981	37.0	34.0	39.0	30.0	39.0
14-15	36.52133200016637	40.0	34.0	41.0	29.5	41.0
16-17	36.31605209358316	39.5	34.0	40.5	28.5	41.0
18-19	35.24200777714531	36.5	33.5	40.0	27.0	41.0
20-21	34.40632125271401	35.0	32.5	39.5	27.0	40.5
22-23	33.56690968956403	35.0	32.0	37.0	27.0	39.5
24-25	32.846358267608124	35.0	32.0	35.0	25.0	39.0
26-27	32.40609312864283	35.0	30.5	35.0	25.0	39.0
28-29	32.239659271432714	35.0	30.0	35.0	25.0	39.0
30-31	32.12053749167579	35.0	30.0	35.0	25.0	39.0
32-33	31.99105991756484	35.0	30.0	35.0	25.0	39.0
34-35	31.90235864922228	35.0	30.0	35.0	24.0	38.0
36-37	31.755664198220334	35.0	30.0	35.0	24.0	38.0
38-39	31.631509737638176	35.0	30.0	35.0	23.5	38.0
40-41	31.48356761921076	34.5	28.5	35.0	23.0	38.0
42-43	31.348016736686077	34.0	28.0	35.0	23.0	38.0
44-45	31.20086921066659	34.0	28.0	35.0	22.5	38.0
46-47	31.134204218850364	34.0	27.0	35.0	22.0	38.0
48-49	31.072949168796026	34.0	27.0	35.0	22.0	37.0
50-51	30.999050134270384	33.5	27.0	35.0	22.0	37.0
52-53	30.85988469546315	33.0	27.0	35.0	22.0	37.0
54-55	30.853260574543285	33.0	27.5	35.0	22.0	36.5
56-57	30.817296561659663	33.0	28.0	35.0	21.5	36.0
58-59	30.653023287746457	33.0	28.0	35.0	21.0	35.5
60-61	30.4991552145271	33.0	28.0	35.0	21.0	35.0
62-63	30.454902291620236	33.0	28.0	35.0	21.0	35.0
64-65	30.58422590361809	33.0	28.0	35.0	21.5	35.0
66-67	30.837331304491535	33.0	29.0	35.0	22.0	35.0
68-69	30.829602569003796	33.0	29.0	35.0	22.0	35.0
70-71	31.01939107053311	33.0	29.0	35.0	24.0	35.0
72-73	31.026954147927093	33.0	29.0	35.0	24.0	35.0
74-75	30.980803512822227	33.0	29.0	35.0	24.0	35.0
76-77	30.920590926048412	33.0	29.0	35.0	24.0	35.0
78-79	30.860229726892555	33.0	29.0	35.0	24.0	35.0
80-81	30.83014677301862	33.0	29.0	35.0	24.0	35.0
82-83	30.800457994672193	33.0	29.0	35.0	24.0	35.0
84-85	30.767193262024215	33.0	29.0	35.0	24.0	35.0
86-87	30.694707631690317	33.0	29.0	35.0	23.0	35.0
88-89	30.650457383648988	33.0	29.0	35.0	23.0	35.0
90-91	30.563467321194025	33.0	29.0	35.0	23.0	35.0
92-93	30.47126588640664	33.0	29.0	35.0	22.0	35.0
94-95	30.336444193205224	33.0	29.0	35.0	20.0	35.0
96-97	30.198604910769895	33.0	29.0	35.0	20.0	35.0
98-99	30.03569041609841	33.0	29.0	35.0	19.5	35.0
100	29.25637673519445	33.0	28.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.9078735282881389
1101	2	0.7030544610063565
1101	3	0.6476395325900448
1101	4	0.584892847927108
1101	5	0.4794695025553608
1101	6	0.4379197361240088
1101	7	0.45811187501725215
1101	8	0.48626477430985204
1101	9	0.508305762877157
1101	10-11	0.517069030238055
1101	12-13	0.6197435614175504
1101	14-15	0.7390066193332459
1101	16-17	0.7225352321155043
1101	18-19	0.7221774246670805
1101	20-21	0.6453328105254457
1101	22-23	0.5774983005326604
1101	24-25	0.5757771706986929
1101	26-27	0.6905434636744303
1101	28-29	0.5702556211399212
1101	30-31	0.5438412067432807
1101	32-33	0.6085509864206173
1101	34-35	0.5751479518311022
1101	36-37	0.5037855256399304
1101	38-39	0.5115362455338648
1101	40-41	0.5346957335386797
1101	42-43	0.5069721613604194
1101	44-45	0.46368345304138003
1101	46-47	0.5077948200809992
1101	48-49	0.4765364908746079
1101	50-51	0.4934026715250006
1101	52-53	0.48579581956921203
1101	54-55	0.4368160084694708
1101	56-57	0.3931452048671531
1101	58-59	0.4099402025331145
1101	60-61	0.38610643287934465
1101	62-63	0.3917319621401312
1101	64-65	0.4510211275147249
1101	66-67	0.45824388815636397
1101	68-69	0.46011335546125665
1101	70-71	0.44054939530473547
1101	72-73	0.45880389063745497
1101	74-75	0.4091783380497702
1101	76-77	0.4198842063062891
1101	78-79	0.39102475992772057
1101	80-81	0.38445621258848917
1101	82-83	0.38240010041381467
1101	84-85	0.3514513430448183
1101	86-87	0.3539646298588508
1101	88-89	0.3484172267999348
1101	90-91	0.39103383889718657
1101	92-93	0.3319051364434529
1101	94-95	0.35435445871128834
1101	96-97	0.3598984302989727
1101	98-99	0.35159058433438517
1101	100	0.3333314699337464
1102	1	0.6338789009921832
1102	2	0.4906274402362705
1102	3	0.4233656120449112
1102	4	0.4155584953816245
1102	5	0.37427348621884704
1102	6	0.3397240037108773
1102	7	0.33049145874188923
1102	8	0.3965171166777708
1102	9	0.4157622024779144
1102	10-11	0.3501643411341675
1102	12-13	0.3904873533235502
1102	14-15	0.5290885639900225
1102	16-17	0.550455165721111
1102	18-19	0.5578145050610743
1102	20-21	0.44326206992222694
1102	22-23	0.3937808698208869
1102	24-25	0.3974246688968748
1102	26-27	0.4708320390342493
1102	28-29	0.3748640907928831
1102	30-31	0.37899306207339833
1102	32-33	0.3417271062799614
1102	34-35	0.3502970552884115
1102	36-37	0.3497772700215158
1102	38-39	0.3553888237765932
1102	40-41	0.3493820637197409
1102	42-43	0.39107917863606545
1102	44-45	0.36056169359000734
1102	46-47	0.32614867597896335
1102	48-49	0.31968397815265703
1102	50-51	0.31898366613046036
1102	52-53	0.332400270614265
1102	54-55	0.266199851044874
1102	56-57	0.21772556610505944
1102	58-59	0.26014137317107355
1102	60-61	0.2734835704948253
1102	62-63	0.30603431870422426
1102	64-65	0.29556040722293986
1102	66-67	0.29012658411809866
1102	68-69	0.30871590640366264
1102	70-71	0.33359102284093467
1102	72-73	0.33265526766586717
1102	74-75	0.32771014838894885
1102	76-77	0.3356349215748189
1102	78-79	0.3089646118507545
1102	80-81	0.3075884990453446
1102	82-83	0.2808133181393693
1102	84-85	0.24131420805017
1102	86-87	0.2837626074507398
1102	88-89	0.33362730826410214
1102	90-91	0.3788075082793263
1102	92-93	0.33966487173949744
1102	94-95	0.35879259617388826
1102	96-97	0.35130649372308653
1102	98-99	0.3366865504383014
1102	100	0.3342378844182079
1103	1	0.37317652446814975
1103	2	0.2935289645642811
1103	3	0.2756692976379185
1103	4	0.2687238369379443
1103	5	0.23510438975554848
1103	6	0.21843092247711837
1103	7	0.26972668606672556
1103	8	0.34524897754855033
1103	9	0.3551944145869541
1103	10-11	0.3148710761447049
1103	12-13	0.40337822412524815
1103	14-15	0.48083647898365456
1103	16-17	0.46825093831366615
1103	18-19	0.4770066156839903
1103	20-21	0.3406055412726374
1103	22-23	0.24972526567767517
1103	24-25	0.28282387020521327
1103	26-27	0.3329756806980555
1103	28-29	0.23579523951927683
1103	30-31	0.15739711304225779
1103	32-33	0.19061196247623258
1103	34-35	0.21598268438432555
1103	36-37	0.18683013190063846
1103	38-39	0.15463640610618157
1103	40-41	0.17993425356619142
1103	42-43	0.16563654079156365
1103	44-45	0.2100095428123332
1103	46-47	0.2443900617684207
1103	48-49	0.18571770609721483
1103	50-51	0.19857397303392688
1103	52-53	0.20080795199976365
1103	54-55	0.15734517448718321
1103	56-57	0.11398837052492894
1103	58-59	0.09703723087066152
1103	60-61	0.15113586398942758
1103	62-63	0.16617057206459407
1103	64-65	0.2030994830377253
1103	66-67	0.20760522814672555
1103	68-69	0.23766158739152132
1103	70-71	0.18652103658619268
1103	72-73	0.17516696040515356
1103	74-75	0.20337129692165945
1103	76-77	0.19525883226358687
1103	78-79	0.1692236835467682
1103	80-81	0.19652870113357324
1103	82-83	0.16371007740069743
1103	84-85	0.16663991634975517
1103	86-87	0.15644238632807372
1103	88-89	0.1417008403416382
1103	90-91	0.20187259703084592
1103	92-93	0.2337842357991029
1103	94-95	0.26246406349956075
1103	96-97	0.20245153308902175
1103	98-99	0.18357296110844956
1103	100	0.14880375429698844
1104	1	0.3281098900614978
1104	2	0.23793765600255767
1104	3	0.2011977533089535
1104	4	0.25795714475515297
1104	5	0.23527017821648144
1104	6	0.24105591952096717
1104	7	0.22210210984806622
1104	8	0.37624707849982997
1104	9	0.2923234093946334
1104	10-11	0.27533832786182444
1104	12-13	0.3330045261390424
1104	14-15	0.46479372991042567
1104	16-17	0.49887560870787695
1104	18-19	0.47783552591980083
1104	20-21	0.3744832108079521
1104	22-23	0.2787024449172151
1104	24-25	0.2639228632486734
1104	26-27	0.36406004436770445
1104	28-29	0.21942430716414663
1104	30-31	0.2186444158860681
1104	32-33	0.2461469071867839
1104	34-35	0.2576628125838063
1104	36-37	0.21363922605840102
1104	38-39	0.18619000888195103
1104	40-41	0.18430916102630945
1104	42-43	0.19770553730870333
1104	44-45	0.19150840498716803
1104	46-47	0.19023330147130935
1104	48-49	0.21520397660392376
1104	50-51	0.22556513405751488
1104	52-53	0.18517115696692343
1104	54-55	0.14713638111897964
1104	56-57	0.17973501177872464
1104	58-59	0.12947883861692588
1104	60-61	0.1791871957459925
1104	62-63	0.22299104041822204
1104	64-65	0.2954070985349375
1104	66-67	0.2982142070590683
1104	68-69	0.2407561638486868
1104	70-71	0.2371308709196569
1104	72-73	0.21676115600687496
1104	74-75	0.2017668692156498
1104	76-77	0.22264528600358702
1104	78-79	0.18613012247774208
1104	80-81	0.16332009994587082
1104	82-83	0.18296648969974783
1104	84-85	0.17503840244552293
1104	86-87	0.16929832072816353
1104	88-89	0.14020160038305463
1104	90-91	0.1679408342454103
1104	92-93	0.15008055397767706
1104	94-95	0.15656034230386595
1104	96-97	0.14749571049041066
1104	98-99	0.20136020523643694
1104	100	0.2272355701878297
1105	1	0.28846336273745266
1105	2	0.2616200692666091
1105	3	0.21372891656201531
1105	4	0.229128217745604
1105	5	0.19737632806467076
1105	6	0.1800844419529426
1105	7	0.15983434248828132
1105	8	0.2266295332379613
1105	9	0.2659721094806571
1105	10-11	0.2356214902584881
1105	12-13	0.257934129627472
1105	14-15	0.393943138582884
1105	16-17	0.34908655776384023
1105	18-19	0.3750920362019201
1105	20-21	0.24487999200712096
1105	22-23	0.16889364386607042
1105	24-25	0.14258936204173267
1105	26-27	0.2155593061222305
1105	28-29	0.15961202038560884
1105	30-31	0.12234856842946584
1105	32-33	0.15588150718244265
1105	34-35	0.14869904172828186
1105	36-37	0.13029139355656483
1105	38-39	0.0856745572475468
1105	40-41	0.06766008088669295
1105	42-43	0.08230009668822547
1105	44-45	0.07951486113727668
1105	46-47	0.06944093818749053
1105	48-49	0.08955546005905646
1105	50-51	0.11056449994662287
1105	52-53	0.07395293665933167
1105	54-55	0.08554416473124249
1105	56-57	0.07573947732343811
1105	58-59	0.07914363127395774
1105	60-61	0.07570555588590366
1105	62-63	0.0833323895642728
1105	64-65	0.14707650811684303
1105	66-67	0.0808455810881128
1105	68-69	0.10749509195180451
1105	70-71	0.11814384236793174
1105	72-73	0.08408383717591761
1105	74-75	0.10398727088415427
1105	76-77	0.15001340417571996
1105	78-79	0.1494416483960812
1105	80-81	0.11448164876313172
1105	82-83	0.08606323724160347
1105	84-85	0.08666025480332351
1105	86-87	0.09279096771376061
1105	88-89	0.09951879909803552
1105	90-91	0.07680295242405677
1105	92-93	0.10171084021686738
1105	94-95	0.1264969036442345
1105	96-97	0.11462503459596718
1105	98-99	0.10761814801891134
1105	100	0.18766162372403272
1106	1	0.0637446547483762
1106	2	0.11804137929873093
1106	3	0.17185202040057135
1106	4	0.1547519387227112
1106	5	0.1576900357863522
1106	6	0.051500639499124645
1106	7	0.10688598217305412
1106	8	0.23518261347035718
1106	9	0.22287984410883155
1106	10-11	0.14877512204746068
1106	12-13	0.22040160257505192
1106	14-15	0.2555809928548811
1106	16-17	0.21962971482948035
1106	18-19	0.2841033796671155
1106	20-21	0.17922930067636855
1106	22-23	0.13272968176330835
1106	24-25	0.12591553388529775
1106	26-27	0.2193648291935233
1106	28-29	0.09351281470738826
1106	30-31	0.07201895096537214
1106	32-33	0.11646490473914639
1106	34-35	0.09603276017107376
1106	36-37	0.10716123106062625
1106	38-39	0.05211937565766789
1106	40-41	0.038940249789103376
1106	42-43	0.11180423747300594
1106	44-45	0.0788303623250286
1106	46-47	0.1025542397774899
1106	48-49	0.11569403958062452
1106	50-51	0.09512460275131573
1106	52-53	0.06307912197927479
1106	54-55	0.08924693113303661
1106	56-57	0.048338125456851344
1106	58-59	0.06609971428706984
1106	60-61	0.060983084058374715
1106	62-63	0.05504361798987745
1106	64-65	0.09164574800464109
1106	66-67	0.07653460005444046
1106	68-69	0.061668602813359286
1106	70-71	0.09952454947169542
1106	72-73	0.08305010847007921
1106	74-75	0.0914615974012456
1106	76-77	0.1199655425499806
1106	78-79	0.09928815483229414
1106	80-81	0.13320102295608294
1106	82-83	0.06430871032337393
1106	84-85	0.08897558442107467
1106	86-87	0.11065581269818736
1106	88-89	0.0976348607208557
1106	90-91	0.09620984694190682
1106	92-93	0.12112479094028217
1106	94-95	0.13246148860262608
1106	96-97	0.12298453249815822
1106	98-99	0.1221326042046158
1106	100	0.08673995708130988
1107	1	-0.04395089847492173
1107	2	0.049964916913225466
1107	3	0.06193169275741539
1107	4	0.12828946499806904
1107	5	0.06452893115046976
1107	6	0.013114434430107735
1107	7	0.003889834851527496
1107	8	0.17694569834524998
1107	9	0.14413906979824276
1107	10-11	0.1242497824349087
1107	12-13	0.15283902821153106
1107	14-15	0.2516272201888583
1107	16-17	0.21382483666632623
1107	18-19	0.26535519092595194
1107	20-21	0.16053266764587448
1107	22-23	0.10260359262930763
1107	24-25	0.11115379314756524
1107	26-27	0.20499330494098444
1107	28-29	0.05881608135418048
1107	30-31	0.05697581818188979
1107	32-33	0.12257857363321989
1107	34-35	0.10465963067235506
1107	36-37	0.08221003861359932
1107	38-39	0.03482670158235024
1107	40-41	0.1079804300630407
1107	42-43	0.08099651286622489
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2216	58-59	0.5642750305602178
2216	60-61	0.5837384018687537
2216	62-63	0.42832559974286966
2216	64-65	0.38218558580639694
2216	66-67	0.42648559699053834
2216	68-69	0.430295365818921
2216	70-71	0.3917941648274095
2216	72-73	0.3897366025080409
2216	74-75	0.3560143126905366
2216	76-77	0.35807614416796696
2216	78-79	0.34839537979568647
2216	80-81	0.337809739893693
2216	82-83	0.33682201761889274
2216	84-85	0.2445082068042943
2216	86-87	0.20703946351608593
2216	88-89	0.15051526849230257
2216	90-91	0.14658291643571886
2216	92-93	0.10611802695437689
2216	94-95	0.04387673423513405
2216	96-97	0.04545908877518201
2216	98-99	0.017957228237673206
2216	100	0.15169162228415445
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	133.0
6	2143.0
7	7625.0
8	11759.0
9	17539.0
10	26303.0
11	33663.0
12	39056.0
13	44257.0
14	50753.0
15	60916.0
16	72350.0
17	87974.0
18	106295.0
19	125442.0
20	147884.0
21	172046.0
22	194996.0
23	235738.0
24	349271.0
25	425412.0
26	430481.0
27	872642.0
28	558636.0
29	1212362.0
30	827099.0
31	926280.0
32	1225441.0
33	2774006.0
34	1715004.0
35	6039775.0
36	215620.0
37	48992.0
38	7393.0
39	1088.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.750053104195938	43.585684472438004	23.084574922546548	19.57968750081951
2	9.270371566975834	53.05689416079067	21.72202443266332	15.950709839570173
3	7.681001824104583	59.070316638100614	18.401024087421913	14.847657450372884
4	6.146873750254047	61.48534789649317	16.984801777999227	15.382976575253554
5	6.181250116370214	59.73225016593409	17.98444953020525	16.102050187490445
6	6.1770459928442625	56.75343493381727	19.738026816733267	17.3314922566052
7	7.916615944747559	62.300691704491975	15.980816283993935	13.801876066766534
8	7.595710753468186	58.80408650221418	14.499155817683054	19.101046926634574
9	6.4283260077857465	57.55078087978844	14.578945483041117	21.44194762938469
10-11	7.296330362466833	71.28489106400329	13.2717701667422	8.147008406787675
12-13	6.897752249176108	71.6596690492299	13.487383453499973	7.95519524809402
14-15	7.249968649002519	71.23709226592003	13.229266028961243	8.283673056116214
16-17	7.211343066242415	71.16332104800811	13.406659439634552	8.218676446114912
18-19	7.3406020094324145	70.75174840487541	13.797939818567542	8.109709767124633
20-21	7.172578318188213	70.78344609075481	13.821315512429017	8.22266007862796
22-23	7.298408082458456	70.97503107171332	14.069204128293489	7.657356717534746
24-25	7.2565349679896824	70.83350553897253	13.992228298313176	7.917731194724613
26-27	7.800617187228538	70.78686979080277	13.720128472497667	7.692384549471025
28-29	7.637971006450674	71.12536418163093	13.817825608103144	7.41883920381526
30-31	7.783032635614765	71.13542377396358	13.767349322071066	7.314194268350588
32-33	7.9174656079164265	71.23448558323528	13.54573671277593	7.302312096072358
34-35	7.897417228515178	71.34159296843937	13.500534107708653	7.260455695336803
36-37	8.146827460001713	71.20528759026557	13.33729650501545	7.31058844471727
38-39	8.29269154882529	71.25526823944914	13.395469585493922	7.05657062623165
40-41	8.355325806084137	71.38518801584276	13.408303373080102	6.851182804993
42-43	8.4567205338552	71.45227472132409	13.509483990094914	6.581520754725788
44-45	8.506210763876478	71.41908389557753	13.42546533951696	6.649240001029037
46-47	8.48871025164578	71.28320330652096	13.519881965710853	6.708204476122404
48-49	8.432079815065007	71.29340865021776	13.649240779926943	6.625270754790286
50-51	8.53925802520778	71.19124712507629	13.7864692741418	6.48302557557413
52-53	8.631835878009031	71.11116303497279	13.906466296155823	6.350534790862356
54-55	8.660567989937679	71.06664184461059	13.935267144231627	6.337523021220104
56-57	8.632995665615969	71.06152626689382	13.77909395161965	6.526384115870572
58-59	8.530585689975528	70.96259852849326	13.819952302942948	6.686863478588262
60-61	8.51441167722513	70.88808787447265	13.945382602538217	6.6521178457640024
62-63	8.527456698445036	70.62407741712224	14.066023504780786	6.782442379651949
64-65	8.49526863019475	70.58999200686445	14.039441718293372	6.875297644647431
66-67	8.283137771015191	70.53889750806178	14.270882615746434	6.90708210517659
68-69	8.14462885339174	70.67578326598945	14.138753571743262	7.040834308875546
70-71	7.861973259144177	70.90153194247722	14.1466751784052	7.08981961997341
72-73	7.78319737230742	71.1265753868099	14.124322276421664	6.965904964461004
74-75	7.704506477335574	71.34643135580448	14.044790702127688	6.904271464732256
76-77	7.478098450775268	71.70469215100275	14.05759756955231	6.7596118286696765
78-79	7.257506846720653	72.09734460291853	13.91947952833606	6.725669022024762
80-81	7.0326552212636315	72.5893375544223	13.731045616398049	6.64696160791601
82-83	6.835170648359446	72.98061217120176	13.655833900700493	6.528383279738313
84-85	6.641457313322253	73.47397181427753	13.503709980317655	6.380860892082567
86-87	6.526221109873566	73.82425630762084	13.337554992300547	6.311967590205049
88-89	6.409960070135577	74.09127957566193	13.325851400902028	6.172908953300468
90-91	6.398938212395285	74.30791316041784	13.226488464257766	6.066660162929115
92-93	6.426774717374315	74.45166291290514	13.06718642646684	6.05437594325371
94-95	6.548890605191122	74.42991409371467	12.919053779168758	6.1021415219254544
96-97	6.682483282043148	74.31291659835144	12.858571051985349	6.146029067620069
98-99	6.805722435281342	74.17724605527098	12.81573442334798	6.201297086099693
100	7.1356176722744875	73.72769826090413	12.829247742880378	6.3074363239409985
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4729573.0
1	4466042.5
2	2604904.5
3	599544.0
4	176392.0
5	153051.0
6	127290.5
7	90413.5
8	58983.0
9	42340.5
10	38631.5
11	42940.0
12	52702.5
13	67410.0
14	87662.5
15	112494.0
16	139355.5
17	165938.0
18	191446.0
19	214200.0
20	233053.0
21	246798.5
22	256174.5
23	264595.5
24	270116.0
25	271111.5
26	267685.0
27	259208.5
28	247431.0
29	232767.5
30	216505.5
31	200588.0
32	184862.0
33	170376.0
34	157720.5
35	146750.5
36	137194.0
37	128808.5
38	122335.0
39	117425.0
40	114152.5
41	114541.0
42	119367.0
43	128989.5
44	143156.5
45	161571.0
46	181986.5
47	200715.5
48	213435.5
49	218366.5
50	216848.0
51	209069.5
52	195613.5
53	178192.0
54	158032.0
55	134201.5
56	107774.5
57	81939.5
58	59206.5
59	41144.5
60	28413.5
61	20182.0
62	14963.0
63	11658.5
64	9405.5
65	7820.5
66	6765.5
67	5928.0
68	5260.5
69	4636.0
70	4023.5
71	3547.0
72	3196.5
73	2862.5
74	2549.5
75	2313.0
76	2132.0
77	1935.0
78	1735.0
79	1625.0
80	1477.0
81	1334.5
82	1226.0
83	1144.5
84	1092.5
85	1040.5
86	1034.0
87	1013.0
88	972.5
89	898.5
90	856.5
91	831.5
92	794.0
93	776.0
94	744.5
95	711.5
96	668.0
97	637.0
98	583.0
99	517.5
100	493.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.6679025944045276E-4
2	5.19238603197807E-4
3	1.993037062779461E-4
4	2.0979337502941698E-5
5	3.881177438044214E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.4581092193751106E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.409142344228026E-5
22-23	1.0489668751470849E-5
24-25	1.4161052814485646E-4
26-27	2.1766062659302012E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	3.671384063014797E-5
42-43	5.5070760945221954E-5
44-45	2.3601754690809408E-5
46-47	2.884658906654483E-4
48-49	1.8356920315073985E-5
50-51	1.8356920315073985E-5
52-53	2.6224171878677123E-6
54-55	1.311208593933856E-5
56-57	1.0489668751470849E-5
58-59	1.8356920315073985E-5
60-61	0.0
62-63	1.311208593933856E-5
64-65	9.965185313897305E-5
66-67	1.8619162033860757E-4
68-69	3.0944522816839E-4
70-71	8.417959173055356E-4
72-73	3.6451598911361197E-4
74-75	8.785097579356836E-4
76-77	0.0013741466064426811
78-79	7.054302235364146E-4
80-81	0.0010961703845287036
82-83	0.0012692499189279726
84-85	0.001612786570538643
86-87	0.0021739838487423334
88-89	0.0023549306347052054
90-91	0.002197585603433143
92-93	0.002058597492476154
94-95	0.0010253651204562755
96-97	0.0018514265346346048
98-99	0.0017596419330592346
100	0.0013898811095698874
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9066379E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.45686214248465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.36763729591813	36.55991044223015
2	7.680276356968275	6.214397635800995
3	1.2201398554864025	1.480890897838936
4	0.2474898200363724	0.4005064612351942
5	0.11016907581161833	0.22285475562377915
6	0.06414956028801432	0.1557173950243922
7	0.04875012706840676	0.13805940191645993
8	0.03607589128780403	0.11676138883983606
9	0.02818311265734438	0.10261802731718662
>10	0.1692518853374408	1.2603186416216328
>50	0.013145788988246903	0.3678167723991534
>100	0.009730615100527881	0.8101289122389742
>500	0.0015222395660031117	0.43074438383379593
>1k	0.0019309616336158435	1.8745901785013341
>5k	4.2322430133342695E-4	1.248725973018634
>10k+	0.0011241895504169154	48.615958732559555
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2099019	11.009007006521793	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	621006	3.257073616337953	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	450005	2.3602016932528196	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	430301	2.256857476713329	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	297933	1.5626092400659823	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	285826	1.4991100302789533	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	229042	1.201287355087193	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	220839	1.1582639787030353	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	211782	1.1107615137619997	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	204710	1.0736700450567986	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	190384	0.9985325477900131	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	181038	0.9495143257143898	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	179126	0.9394862023879836	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	170752	0.8955659593255751	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	166212	0.8717544112597363	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	154041	0.8079195320726604	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	137699	0.7222084487043922	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	127714	0.669838777462674	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124531	0.6531444696447082	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	121098	0.6351389532328084	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	121013	0.6346931423108709	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	106287	0.5574577112937911	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95569	0.5012435764546588	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93303	0.4893587817592423	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	85985	0.4509770837976104	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82651	0.43349080598890855	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82422	0.4322897389168651	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73079	0.38328725134436903	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58924	0.3090466207558341	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57432	0.3012213278672369	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55099	0.2889851292686461	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54385	0.28524031752437107	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53234	0.27920351315789954	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52448	0.2750810733385715	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52010	0.2727838358819994	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51603	0.2706491882910751	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49999	0.26223647395239547	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46566	0.24423095754049579	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46203	0.24232708266210382	No Hit
TATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46016	0.24134629863384127	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45952	0.2410106292337942	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45935	0.24092146704940673	No Hit
TAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45161	0.23686196524258749	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44789	0.23491088685481393	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43932	0.23041606379480867	No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39139	0.20527757263190874	No Hit
TAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38140	0.20003798309054907	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36153	0.18961649718596277	No Hit
GAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35659	0.18702554900434948	No Hit
TAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35281	0.1850430016103215	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35140	0.1843034799633428	No Hit
TAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33185	0.17404982875878003	No Hit
TAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32603	0.17099733515210203	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25297	0.13267857520297902	No Hit
GAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24265	0.12726590612722005	No Hit
ATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24032	0.1260438597176737	No Hit
CTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23462	0.12305430412350453	No Hit
ACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23333	0.12237772048903466	No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23169	0.12151756765141405	No Hit
CATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22550	0.11827101517283382	No Hit
AAAAATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22503	0.11802450795717426	No Hit
AAAAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22204	0.11645630247882936	No Hit
AAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21971	0.115234256069283	No Hit
GAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21588	0.11322548450337633	No Hit
ATAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21205	0.11121671293746968	No Hit
GAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21036	0.11033033592797038	No Hit
AAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20445	0.10723063881191075	No Hit
AAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20193	0.10590894054922542	No Hit
AAAAACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19693	0.1032865233613577	No Hit
CAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19550	0.10253651204562755	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0015157571345875375	0.0	0.0	5.2448343757354245E-6	0.0
2	0.003750056578650828	0.0	0.0	1.0489668751470849E-5	0.0
3	0.005821766157066321	0.0	0.0	1.573450312720627E-5	0.0
4	0.009162725654409785	0.0	0.0	2.6224171878677122E-5	0.0
5	0.013993218114462112	0.0	0.0	3.671384063014797E-5	0.0
6	0.02250033947190497	0.0	0.0	4.1958675005883396E-5	0.0
7	0.033682326360972896	0.0	0.0	4.7203509381618817E-5	0.0
8	0.06824578489706934	0.0	0.0	4.7203509381618817E-5	0.0
9	0.18738744257627524	0.0	0.0	4.7203509381618817E-5	0.0
10-11	0.4367688274737432	0.0	0.0	4.7203509381618817E-5	0.0
12-13	0.7529274436430746	0.0	0.0	4.9825926569486533E-5	0.0
14-15	1.1214504862197483	0.0	0.0	6.55604296966928E-5	0.0
16-17	1.5187099763410767	0.0	0.0	8.129493282389908E-5	0.0
18-19	1.9411105800424926	0.0	0.0	1.1276393907831162E-4	0.0
20-21	2.4977238730017906	0.0	0.0	1.1538635626617933E-4	0.0
22-23	3.1170050694995624	0.0	0.0	1.1538635626617933E-4	0.0
24-25	3.754866091773378	0.0	0.0	1.1538635626617933E-4	0.0
26-27	4.3612738422959065	0.0	0.0	1.1538635626617933E-4	0.0
28-29	4.938187266706489	0.0	0.0	1.2063119064191475E-4	0.0
30-31	5.520324546155303	0.0	0.0	1.2587602501765017E-4	0.0
32-33	6.181766343782424	0.0	0.0	1.2587602501765017E-4	0.0
34-35	6.810482997322145	0.0	0.0	1.3112085939338562E-4	0.0
36-37	7.3376675245991905	0.0	0.0	1.3636569376912104E-4	0.0
38-39	7.734869321542386	0.0	0.0	1.5734503127206272E-4	0.0
40-41	8.06980444477685	0.0	0.0	1.73079534399269E-4	0.0
42-43	8.334663335917114	2.6224171878677123E-6	0.0	1.8356920315073985E-4	0.0
44-45	8.547509204553208	5.2448343757354245E-6	0.0	1.966812890900784E-4	0.0
46-47	8.728062627937902	5.2448343757354245E-6	0.0	2.0717095784154925E-4	0.0
48-49	8.893830863217394	5.2448343757354245E-6	0.0	2.4388479847169722E-4	0.0
50-51	9.063152473786449	5.2448343757354245E-6	0.0	2.7010897035037435E-4	0.0
52-53	9.241151662830157	5.2448343757354245E-6	0.0	2.884658906654483E-4	0.0
54-55	9.425691160340408	5.2448343757354245E-6	0.0	3.1206764535625776E-4	0.0
56-57	9.625055182213675	5.2448343757354245E-6	0.0	3.199348969198609E-4	0.0
58-59	9.834830200322777	5.2448343757354245E-6	0.0	3.487814859864057E-4	0.0
60-61	10.050697093559297	5.2448343757354245E-6	0.0	3.6451598911361197E-4	0.0
62-63	10.251332987768679	5.2448343757354245E-6	0.0	3.8025049224081826E-4	0.0
64-65	10.441001933298399	5.2448343757354245E-6	0.0	4.0909708130736305E-4	0.0
66-67	10.612248398083349	5.2448343757354245E-6	0.0	4.4056608756177563E-4	0.0
68-69	10.762263773315322	5.2448343757354245E-6	0.0	4.720350938161882E-4	0.0
70-71	10.896515798831022	7.867251563603137E-6	0.0	4.772799281919236E-4	0.0
72-73	11.020086719140535	1.0489668751470849E-5	0.0	4.930144313191299E-4	0.0
74-75	11.13798797348988	1.0489668751470849E-5	0.0	5.087489344463361E-4	0.0
76-77	11.253796538923305	1.573450312720627E-5	0.0	5.480851922643518E-4	0.0
78-79	11.367289509979845	1.573450312720627E-5	0.0	5.847990328944998E-4	0.0
80-81	11.476392554663892	1.573450312720627E-5	0.0	6.11023204773177E-4	0.0
82-83	11.585398569911991	1.573450312720627E-5	0.0	6.320025422761185E-4	0.0
84-85	11.697923869026205	1.573450312720627E-5	0.0	6.608491313426634E-4	0.0
86-87	11.811233270879594	1.573450312720627E-5	0.0	6.713388000941343E-4	0.0
88	11.896600817596251	1.573450312720627E-5	0.0	6.713388000941343E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAAACA	8065	0.0	48.602886	1
TGAAAAA	27735	0.0	45.991978	1
GTAAAAA	17865	0.0	45.0929	1
GAAAATA	12170	0.0	40.782486	1
GATAAAA	10120	0.0	39.104973	1
TACCCGA	4875	0.0	37.599728	6
GAAACAA	6185	0.0	36.24758	1
ACCCGAC	5510	0.0	35.143124	7
CCCGACG	5970	0.0	35.033245	8
CTACCCG	4970	0.0	33.76032	5
TAGAAAA	11730	0.0	32.735912	1
TTGCTGA	3005	0.0	32.375805	9
TCGCGAC	280	0.0	31.892628	6
GCTTGCT	945	0.0	31.333107	7
GCAAAAA	14305	0.0	30.818773	1
AGACCCG	340	0.0	30.411942	1
TCACGCC	830	0.0	30.01191	3
CTTGCTG	2065	0.0	29.815763	8
GGCAAAA	2040	0.0	29.720762	1
GTCACGC	270	0.0	29.592531	2
>>END_MODULE