##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248466_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21749327
Sequences flagged as poor quality	0
Sequence length	100
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.168582687639024	33.0	31.0	34.0	30.0	34.0
2	32.45591553246682	34.0	31.0	34.0	30.0	34.0
3	32.589466929252566	34.0	31.0	34.0	31.0	34.0
4	36.112636450773856	37.0	35.0	37.0	35.0	37.0
5	35.97502180182403	37.0	35.0	37.0	35.0	37.0
6	35.869815925798534	37.0	35.0	37.0	35.0	37.0
7	35.90220704300413	37.0	35.0	37.0	35.0	37.0
8	35.759217974882624	37.0	35.0	37.0	33.0	37.0
9	37.419001562669045	39.0	37.0	39.0	34.0	39.0
10-11	37.59989226333302	39.0	37.0	39.0	35.0	39.0
12-13	37.65027869597988	39.0	37.0	39.0	35.0	39.0
14-15	39.07666110772071	41.0	38.0	41.0	36.0	41.0
16-17	38.97065582764929	40.5	38.0	41.0	35.0	41.0
18-19	38.747744539405744	40.0	38.0	41.0	34.5	41.0
20-21	38.41414095249935	40.0	37.5	41.0	34.0	41.0
22-23	38.069309891749754	40.0	36.5	41.0	34.0	41.0
24-25	37.73797212207992	39.5	35.0	41.0	33.5	41.0
26-27	37.520590775061684	39.0	35.0	41.0	33.0	41.0
28-29	37.34040731467231	39.0	35.0	41.0	33.0	41.0
30-31	37.294356234563026	39.0	35.0	41.0	33.0	41.0
32-33	37.21756942180326	39.0	35.0	41.0	33.0	41.0
34-35	37.148801822695475	39.0	35.0	41.0	33.0	41.0
36-37	37.1195990570191	39.0	35.0	41.0	33.0	41.0
38-39	37.003660228199244	39.0	35.0	41.0	33.0	41.0
40-41	36.87210585872381	39.0	35.0	41.0	32.0	41.0
42-43	36.72434340152226	38.5	35.0	41.0	32.0	41.0
44-45	36.55320070363557	38.0	35.0	41.0	31.5	41.0
46-47	36.29221874313628	37.5	35.0	40.0	31.0	41.0
48-49	35.954828855164116	37.0	35.0	40.0	31.0	41.0
50-51	35.61619265276576	36.5	35.0	39.5	30.5	41.0
52-53	35.27449578094991	35.5	35.0	39.0	30.0	41.0
54-55	34.97154941851764	35.0	35.0	39.0	30.0	40.5
56-57	34.619513996915856	35.0	34.0	37.5	29.5	40.0
58-59	34.2746259688863	35.0	34.0	37.0	29.0	40.0
60-61	33.987013184361984	35.0	34.0	36.0	29.0	39.5
62-63	33.71882116168469	35.0	33.0	35.5	28.5	39.0
64-65	33.500515441236416	35.0	33.0	35.0	28.0	39.0
66-67	33.29940289646663	35.0	33.0	35.0	28.0	38.0
68-69	33.12533951050531	35.0	33.0	35.0	27.5	37.0
70-71	32.97486763613421	35.0	33.0	35.0	27.0	36.5
72-73	32.82641580127974	35.0	33.0	35.0	26.5	36.0
74-75	32.70421047970818	35.0	33.0	35.0	26.5	36.0
76-77	32.55964913305134	35.0	33.0	35.0	27.0	35.5
78-79	32.43826659555948	35.0	33.0	35.0	27.0	35.0
80-81	32.34914675750657	35.0	33.0	35.0	26.0	35.0
82-83	32.24330683887368	35.0	32.0	35.0	25.5	35.0
84-85	32.11118645188424	34.5	32.0	35.0	25.0	35.0
86-87	31.967965928325047	34.0	32.0	35.0	25.0	35.0
88-89	31.848553911576204	34.0	32.0	35.0	25.0	35.0
90-91	31.73160254567877	34.0	32.0	35.0	25.0	35.0
92-93	31.561969664624566	34.0	31.5	35.0	24.0	35.0
94-95	31.318558707586675	34.0	31.0	35.0	23.5	35.0
96-97	31.00970144961267	34.0	31.0	35.0	21.5	35.0
98-99	30.6841241110587	34.0	31.0	35.0	19.5	35.0
100	29.282980250377403	33.0	27.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3100273283184052
1101	2	0.28868584952792986
1101	3	0.3050079848755587
1101	4	0.2059514310243813
1101	5	0.18679923570989843
1101	6	0.158498691458135
1101	7	0.20396775117805532
1101	8	0.13128348744110951
1101	9	0.19377152724914737
1101	10-11	0.19090665393069628
1101	12-13	0.22856577124985478
1101	14-15	0.34664290839941003
1101	16-17	0.42707867059574056
1101	18-19	0.47169336719719723
1101	20-21	0.44822841977029526
1101	22-23	0.43583454919320275
1101	24-25	0.43431122591264426
1101	26-27	0.43452534818545985
1101	28-29	0.46879263348441214
1101	30-31	0.4962557120068425
1101	32-33	0.49870483796308207
1101	34-35	0.5196274896865631
1101	36-37	0.5191175665954972
1101	38-39	0.5011318340069408
1101	40-41	0.5406355785716883
1101	42-43	0.5318674000127857
1101	44-45	0.5161821339832997
1101	46-47	0.5210030423503085
1101	48-49	0.5224183245875409
1101	50-51	0.5143587484133576
1101	52-53	0.5411571694330135
1101	54-55	0.49801191441158466
1101	56-57	0.5018537357553896
1101	58-59	0.47930859381001767
1101	60-61	0.48137548964962207
1101	62-63	0.45914405268924696
1101	64-65	0.4609260790961329
1101	66-67	0.44294737919194915
1101	68-69	0.414320268044861
1101	70-71	0.3830036569974169
1101	72-73	0.3779383465755686
1101	74-75	0.38375420292049256
1101	76-77	0.4187344600705316
1101	78-79	0.391766245768018
1101	80-81	0.32915214328505726
1101	82-83	0.30335379363535253
1101	84-85	0.26680286975409473
1101	86-87	0.3487632634379949
1101	88-89	0.28281540850682063
1101	90-91	0.2469778427011846
1101	92-93	0.2628931380343751
1101	94-95	0.23546109844287955
1101	96-97	0.220461890001296
1101	98-99	0.20070772748167798
1101	100	0.0017335225019152745
1102	1	0.2964237890788155
1102	2	0.27850603795712203
1102	3	0.2651764295721577
1102	4	0.18572601492439134
1102	5	0.16904821539356618
1102	6	0.13918454270754665
1102	7	0.19650884226992815
1102	8	-0.06086855619746956
1102	9	0.0467180830995062
1102	10-11	0.19017944161491585
1102	12-13	0.22006646873872882
1102	14-15	0.3476442302932625
1102	16-17	0.40929086126026704
1102	18-19	0.44038787897488874
1102	20-21	0.4130744804989206
1102	22-23	0.36431875644430534
1102	24-25	0.35799777789942056
1102	26-27	0.3656804824373694
1102	28-29	0.3814849902514226
1102	30-31	0.4243150575686201
1102	32-33	0.44607057633076863
1102	34-35	0.4277889053718056
1102	36-37	0.412795464267262
1102	38-39	0.4370892125457715
1102	40-41	0.43767348246088034
1102	42-43	0.4414739949030704
1102	44-45	0.4470287835442761
1102	46-47	0.44834857029363917
1102	48-49	0.4340339148416277
1102	50-51	0.42281898134316265
1102	52-53	0.43006596260651264
1102	54-55	0.4060890845315299
1102	56-57	0.3809500803201402
1102	58-59	0.3832920283226713
1102	60-61	0.37958089077365287
1102	62-63	0.36831885270692766
1102	64-65	0.37707369562588866
1102	66-67	0.3605566372473845
1102	68-69	0.3174356573702113
1102	70-71	0.27820424721806347
1102	72-73	0.2691293592767252
1102	74-75	0.2670419765509493
1102	76-77	0.3160373404134802
1102	78-79	0.2667069809408815
1102	80-81	0.25324290399520777
1102	82-83	0.25063714356151934
1102	84-85	0.30000165961204317
1102	86-87	0.3445803445149238
1102	88-89	0.30576778331348464
1102	90-91	0.2490561427089446
1102	92-93	0.2541210522932964
1102	94-95	0.22212632674294852
1102	96-97	0.1402858645041647
1102	98-99	0.12878948856291217
1102	100	0.0017632861317409265
1103	1	0.199690355419051
1103	2	0.21058307118723008
1103	3	0.22097248016824977
1103	4	0.14604491134904407
1103	5	0.17415169326216073
1103	6	0.07714951116643931
1103	7	0.17408553845094588
1103	8	0.12552877476841218
1103	9	0.16302146104141713
1103	10-11	0.18923285928755718
1103	12-13	0.20623464763811228
1103	14-15	0.28956151608132785
1103	16-17	0.3553709458448111
1103	18-19	0.4119519164345178
1103	20-21	0.3832420098856133
1103	22-23	0.34221471353032484
1103	24-25	0.3031183387063834
1103	26-27	0.3161510248733208
1103	28-29	0.32519359301586803
1103	30-31	0.30685627622762723
1103	32-33	0.30501241237368504
1103	34-35	0.30357443717150545
1103	36-37	0.3123249776259769
1103	38-39	0.31023536296592624
1103	40-41	0.30340095410399215
1103	42-43	0.31914171056328655
1103	44-45	0.3085193917688187
1103	46-47	0.31152773583108484
1103	48-49	0.3124091640432525
1103	50-51	0.2970760025017256
1103	52-53	0.30407879581955655
1103	54-55	0.27761205599444594
1103	56-57	0.23177935917364323
1103	58-59	0.23497998937205722
1103	60-61	0.18814449341131478
1103	62-63	0.1913666472090938
1103	64-65	0.1624452671476746
1103	66-67	0.17302784193244491
1103	68-69	0.09048906898387088
1103	70-71	0.09135339298286027
1103	72-73	0.11463482733802977
1103	74-75	0.06556413755260593
1103	76-77	0.016653283980787137
1103	78-79	0.03718983020190336
1103	80-81	0.020146003148404645
1103	82-83	0.00820073722721304
1103	84-85	0.036651172846354996
1103	86-87	0.10882166487438383
1103	88-89	0.046909204344459
1103	90-91	-0.04394641302748781
1103	92-93	-0.02074182300865246
1103	94-95	-0.02742425616166244
1103	96-97	-0.0733684473136762
1103	98-99	-0.14230282670602534
1103	100	-0.24551129442029662
1104	1	0.19201105571291777
1104	2	0.18789564262863934
1104	3	0.1816122464179415
1104	4	0.14219275142767174
1104	5	0.0932219226871851
1104	6	0.07106873620318765
1104	7	0.09283914148097239
1104	8	-0.04020306704655496
1104	9	-0.03552007822918313
1104	10-11	0.11229168192971883
1104	12-13	0.1925732153448294
1104	14-15	0.26768489870934076
1104	16-17	0.3145898718663389
1104	18-19	0.3503581595495575
1104	20-21	0.31919285534727493
1104	22-23	0.3000132285491759
1104	24-25	0.3027282045320234
1104	26-27	0.28639686496931915
1104	28-29	0.27807723243628857
1104	30-31	0.2959534358744804
1104	32-33	0.3050400237604691
1104	34-35	0.3204727171617421
1104	36-37	0.3142609711057176
1104	38-39	0.31933370757187873
1104	40-41	0.3048419435543366
1104	42-43	0.3082808961546206
1104	44-45	0.2947977089347802
1104	46-47	0.3270889922029525
1104	48-49	0.34275127596126964
1104	50-51	0.3459090986432898
1104	52-53	0.33128157901173694
1104	54-55	0.29994547314792186
1104	56-57	0.2917941067679237
1104	58-59	0.2597359467028255
1104	60-61	0.2375296943498313
1104	62-63	0.22868650619981423
1104	64-65	0.26034318608403595
1104	66-67	0.24172772034483359
1104	68-69	0.22188960474800012
1104	70-71	0.18534852034729
1104	72-73	0.1742469529457722
1104	74-75	0.14167033144003227
1104	76-77	0.15064733474170566
1104	78-79	0.1497654231162997
1104	80-81	0.10722887371131407
1104	82-83	0.09405485179695461
1104	84-85	0.10930344645969825
1104	86-87	0.16953939797335948
1104	88-89	0.1238866782813659
1104	90-91	0.11326746275026522
1104	92-93	0.1096407350920856
1104	94-95	0.07776028050875539
1104	96-97	-0.08012783943186719
1104	98-99	-0.10169664144761725
1104	100	-0.21539482899099838
1105	1	0.19845436547288386
1105	2	0.17719569165029014
1105	3	0.1600467836073065
1105	4	0.11995335800505558
1105	5	0.15176065453009357
1105	6	0.131447310842411
1105	7	0.14497178082694973
1105	8	-0.016109461689076454
1105	9	0.10412552833323474
1105	10-11	0.1543851963319014
1105	12-13	0.17525367357368538
1105	14-15	0.24024452181422618
1105	16-17	0.2551626945076251
1105	18-19	0.29043027064321336
1105	20-21	0.2832619915131005
1105	22-23	0.27197196854854866
1105	24-25	0.24835102437731393
1105	26-27	0.26115966270573665
1105	28-29	0.253359627654568
1105	30-31	0.261186594214351
1105	32-33	0.2741254891396139
1105	34-35	0.2906272376432355
1105	36-37	0.2811963759499818
1105	38-39	0.27272848051324416
1105	40-41	0.23686516266938895
1105	42-43	0.2940921040693212
1105	44-45	0.289114265797231
1105	46-47	0.2972826938377082
1105	48-49	0.28900574230861764
1105	50-51	0.3004192483851966
1105	52-53	0.271989993572582
1105	54-55	0.24551570971873815
1105	56-57	0.24385540280466955
1105	58-59	0.2435367230844463
1105	60-61	0.23629872887125458
1105	62-63	0.2414581837440224
1105	64-65	0.2441267206126625
1105	66-67	0.23094467115465278
1105	68-69	0.2175947220682275
1105	70-71	0.20762411746115816
1105	72-73	0.19000542059622916
1105	74-75	0.1840571951756047
1105	76-77	0.19004144535060163
1105	78-79	0.1572198438556498
1105	80-81	0.12233843478974649
1105	82-83	0.09510541819614104
1105	84-85	0.1349261005103557
1105	86-87	0.16916273789686187
1105	88-89	0.1590191147287925
1105	90-91	0.14799376862325175
1105	92-93	0.15431981394436178
1105	94-95	0.1359236608606018
1105	96-97	0.1126421978566654
1105	98-99	0.09859217611168347
1105	100	0.06146361449221871
1106	1	0.12030779674627468
1106	2	0.1305522981713949
1106	3	0.11356024245610286
1106	4	0.08413252032553231
1106	5	0.0897760144520987
1106	6	0.09501953074659042
1106	7	0.10885002297892754
1106	8	-0.0045825094631268826
1106	9	0.09336208479897579
1106	10-11	0.10825685121721307
1106	12-13	0.1510725512971618
1106	14-15	0.18818629967744016
1106	16-17	0.1991750580622309
1106	18-19	0.2018701867268362
1106	20-21	0.20316997290623817
1106	22-23	0.21563658576157252
1106	24-25	0.21477355905528128
1106	26-27	0.18517144242078132
1106	28-29	0.22872229054882354
1106	30-31	0.2208980269745382
1106	32-33	0.21324994796359675
1106	34-35	0.23182734651024361
1106	36-37	0.25270028628373353
1106	38-39	0.2223198394005621
1106	40-41	0.2261448900872125
1106	42-43	0.20432636933047377
1106	44-45	0.22222062438861911
1106	46-47	0.21279110909854637
1106	48-49	0.21951170600375747
1106	50-51	0.2035781378132384
1106	52-53	0.20349826347980837
1106	54-55	0.22144213968389437
1106	56-57	0.18948021623486966
1106	58-59	0.16124575711366873
1106	60-61	0.18303431895377997
1106	62-63	0.1692713259259122
1106	64-65	0.1585542175147694
1106	66-67	0.1864631851740839
1106	68-69	0.21236109044706097
1106	70-71	0.1506534179215464
1106	72-73	0.14665795374823887
1106	74-75	0.1207679902266463
1106	76-77	0.08345980493635352
1106	78-79	0.11843586964801034
1106	80-81	0.060685807201430464
1106	82-83	0.005571953030262478
1106	84-85	0.0432418062703519
1106	86-87	0.07560386445026523
1106	88-89	0.042776904872155797
1106	90-91	0.042263402720422505
1106	92-93	0.06433400102466535
1106	94-95	0.044047101410697564
1106	96-97	0.03799390699865768
1106	98-99	0.04512764577380679
1106	100	-0.027355374565154023
1107	1	0.11257405027468081
1107	2	0.11971866511115081
1107	3	0.112394428215012
1107	4	0.06937182879594417
1107	5	0.09516275060913415
1107	6	0.08066788180936157
1107	7	0.08605563453734533
1107	8	0.07121091953524683
1107	9	0.09770161785902332
1107	10-11	0.10500035851252676
1107	12-13	0.10721477021991177
1107	14-15	0.1526356475492463
1107	16-17	0.14149601071264328
1107	18-19	0.1802052464350865
1107	20-21	0.1651513833797722
1107	22-23	0.17133555606882567
1107	24-25	0.16524047047064272
1107	26-27	0.16187573169447234
1107	28-29	0.14954097576814718
1107	30-31	0.16525796102328627
1107	32-33	0.1660812547806927
1107	34-35	0.1740420866286172
1107	36-37	0.1906103864241473
1107	38-39	0.16295655251357033
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2216	40-41	-0.6760426786539142
2216	42-43	-0.6339181008395514
2216	44-45	-0.6298112285300519
2216	46-47	-0.674729531480267
2216	48-49	-0.7090729109990193
2216	50-51	-0.7577943774095317
2216	52-53	-0.6704686221501035
2216	54-55	-0.5858050260190879
2216	56-57	-0.5287870100578473
2216	58-59	-0.4824195397854112
2216	60-61	-0.48578964981693673
2216	62-63	-0.5078281023293485
2216	64-65	-0.456654346182674
2216	66-67	-0.38764758452222736
2216	68-69	-0.3739547047195231
2216	70-71	-0.3259076363455975
2216	72-73	-0.3099628488328463
2216	74-75	-0.3262109073093953
2216	76-77	-0.39818887732305797
2216	78-79	-0.29030400606435336
2216	80-81	-0.2679273238108735
2216	82-83	-0.33060101161846234
2216	84-85	-0.3130651218333256
2216	86-87	-0.3463929690472227
2216	88-89	-0.3627486667016768
2216	90-91	-0.38071050050579913
2216	92-93	-0.3819905115618063
2216	94-95	-0.4286247679682411
2216	96-97	-0.34687573691896034
2216	98-99	-0.3163309761309243
2216	100	-0.15505097982913796
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	10.0
7	85.0
8	324.0
9	823.0
10	1539.0
11	2641.0
12	3892.0
13	5654.0
14	8328.0
15	12575.0
16	19412.0
17	29228.0
18	41166.0
19	55564.0
20	71608.0
21	90043.0
22	112070.0
23	138004.0
24	167131.0
25	200373.0
26	238246.0
27	283265.0
28	339835.0
29	415399.0
30	479062.0
31	589485.0
32	754706.0
33	1044429.0
34	1483847.0
35	4895309.0
36	3817541.0
37	4789746.0
38	1438982.0
39	218597.0
40	408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.468741608328788	22.991272523946996	30.829225678792195	26.710760188932024
2	16.15130777625298	24.480166491651552	36.563170646678	22.80535508541746
3	13.160416411965297	20.754596222678522	40.324856948447184	25.760130416908993
4	13.525613918996207	18.476153308100063	43.7261208128417	24.27211196006203
5	12.803315874208407	18.857330935721208	45.86620895799376	22.473144232076617
6	11.744565705412402	13.733937606437202	42.13755211827934	32.38394456987106
7	16.068510993466603	14.729807501629821	40.11632176020895	29.085359744694628
8	11.53620523522406	13.328325055759196	40.54664312141704	34.5888265875997
9	11.532315459692155	12.79706723798856	45.48913168669541	30.181485615623878
10-11	15.035582480322265	19.976788247286915	45.266350080625486	19.721279191765337
12-13	13.903241707587124	28.66881196087655	38.015433042033656	19.41251328950267
14-15	15.922677868622603	25.79470959452161	37.83808506546212	20.444527471393673
16-17	23.015411005591115	26.108699823217517	38.96274353684599	11.913145634345375
18-19	22.78802925718115	19.411310979875378	38.221883831164064	19.578775931779408
20-21	16.447620637160444	18.787639699834155	44.40786167469221	20.356877988313194
22-23	16.419452029739414	20.874028285667244	46.01959261345148	16.68692707114186
24-25	16.914133681914368	22.62278337372384	43.20255835550185	17.260524588859944
26-27	21.9420995713168	22.299043987886456	38.665259524389676	17.093596916407066
28-29	19.158873144046552	25.7631747708152	42.20889395525492	12.86905812988332
30-31	19.303223924577598	22.989735842643842	42.3660594042544	15.340980828524165
32-33	20.291324602694505	24.450037182103475	41.32611655453182	13.932521660670194
34-35	20.55359980269385	24.45260785559132	41.238258854154836	13.755533487559992
36-37	20.98075762040336	23.20804447708915	40.68649732071697	15.124700581790524
38-39	21.515777599159776	23.339612398320966	39.76523212378108	15.379377878738179
40-41	21.37609666617529	23.71748119196186	40.07026213497683	14.83616000688602
42-43	21.527811943780666	24.62239273803716	40.123197625203524	13.726597692978654
44-45	21.94844886175035	23.636618121659662	39.42200056400596	14.992932452584023
46-47	22.63062297205248	22.728645895934953	39.93643269943214	14.704298432580432
48-49	21.32971897478691	22.952391447105313	40.27413281983674	15.443756758271038
50-51	21.293169458174855	22.374148198294048	40.4874296202774	15.845252723253694
52-53	20.582414259090122	22.891076890479308	40.98704879077093	15.539460059659646
54-55	20.431889368387388	23.37511612237583	41.11498724059163	15.078007268645155
56-57	20.00561560849484	23.40437839057939	40.52805525909575	16.06195074183002
58-59	19.729219049906707	23.577338313461276	41.229182529591746	15.464260107040273
60-61	18.879180486852963	22.726987960100384	41.93093511359166	16.46289643945499
62-63	18.531307237862617	22.352510365909186	42.46036828522976	16.655814110998435
64-65	18.499823103137206	22.293239477567894	42.45983818184464	16.74709923745026
66-67	17.793020136863515	21.969094657620168	43.182500295934595	17.055384909581722
68-69	17.470065996916958	21.232393505706394	44.01108549023044	17.286455007146202
70-71	16.775807996351126	20.94096924158485	44.85762904975225	17.42559371231178
72-73	16.517662605086112	20.270491310513492	45.932123024245456	17.279723060154943
74-75	16.32658498943713	19.75954184144334	46.573977929027706	17.33989524009182
76-77	16.456744867580124	19.618187277533185	46.62359651831319	17.301471336573503
78-79	16.40941987904449	19.614537650521946	46.79008893863182	17.185953531801744
80-81	16.219375353996053	19.21782496133789	46.95212859493145	17.610671089734605
82-83	16.280159194725805	19.149709003263705	47.085986513626956	17.484145288383537
84-85	16.350642727278327	19.31444508028355	46.90729999184332	17.4276122005948
86-87	16.482586244147402	19.565157618713307	46.723542432723306	17.228713704415984
88-89	16.439173261169582	20.258506981750187	46.471346571513294	16.830973185566933
90-91	16.49008146202949	20.926797372638735	46.142931023812565	16.440190141519206
92-93	16.235200716491317	21.45153585595762	45.7021636284148	16.611099799136262
94-95	16.096092575210115	22.42064605950508	45.324502218123996	16.158759147160808
96-97	15.339865508098779	23.751450911099756	45.55646133329103	15.352222247510433
98-99	14.836873178145016	25.016370328639965	45.249013716867516	14.897742776347506
100	14.453922938932468	26.273594019315617	44.60634232914639	14.666140712605527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	404045.0
1	952160.5
2	1526520.5
3	1091978.5
4	384485.0
5	97185.5
6	46203.0
7	32215.0
8	27568.0
9	27470.0
10	30856.5
11	37336.5
12	47711.5
13	63008.5
14	82325.5
15	105193.5
16	132499.0
17	163406.5
18	194491.0
19	220993.0
20	240568.0
21	254693.0
22	265778.5
23	274687.5
24	280822.0
25	282935.5
26	279379.0
27	271050.0
28	258072.5
29	241596.5
30	223635.0
31	205705.0
32	187920.5
33	169751.5
34	153148.0
35	137839.5
36	123110.5
37	109722.0
38	98151.0
39	88054.0
40	80489.5
41	77035.5
42	80190.5
43	95591.5
44	130478.5
45	188775.0
46	269230.0
47	367290.0
48	484649.0
49	633293.0
50	825288.0
51	1050080.0
52	1262644.0
53	1396938.5
54	1392658.5
55	1235515.5
56	969161.0
57	672900.0
58	419827.5
59	240225.0
60	128825.5
61	65825.5
62	31966.5
63	15109.0
64	7458.5
65	4150.0
66	2677.0
67	2006.0
68	1621.0
69	1276.5
70	987.5
71	742.0
72	572.0
73	453.5
74	333.5
75	246.5
76	175.5
77	103.0
78	61.0
79	41.5
80	37.5
81	35.0
82	25.0
83	13.0
84	6.5
85	9.5
86	12.0
87	10.5
88	7.5
89	6.5
90	6.0
91	2.5
92	3.0
93	4.5
94	1.5
95	0.5
96	0.5
97	0.5
98	2.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0414771454767313
2	0.003351827851960661
3	0.0
4	0.0
5	0.007255396914120607
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	8.276118152989285E-5
14-15	1.3793530254982143E-4
16-17	0.0
18-19	0.0
20-21	7.816333811156547E-5
22-23	3.6782747346619046E-5
24-25	9.172697619563125E-4
26-27	0.001298890765677485
28-29	0.001055205064506134
30-31	0.0011264716374902082
32-33	0.0013264778261874494
34-35	0.0017701697160560418
36-37	0.0031794087237733836
38-39	0.003981732400271512
40-41	0.0041978310409328985
42-43	0.004308179282972756
44-45	0.0061151317463754165
46-47	0.0062208821449969455
48-49	0.006613997757263937
50-51	0.0076990888039892
52-53	0.007485289085036976
54-55	0.005781788098546682
56-57	0.004671408913020619
58-59	0.005322003756713943
60-61	0.0050461331516143005
62-63	0.004567957436108253
64-65	0.003579421101167866
66-67	0.00199316512184492
68-69	0.0016391311786337114
70-71	7.218614166773988E-4
72-73	9.172697619563125E-4
74-75	0.0012069338973109375
76-77	7.195624949682351E-4
78-79	7.954269113706368E-4
80-81	0.0014736088155739256
82-83	5.241541496893215E-4
84-85	0.001328776747896613
86-87	0.001048308299378643
88-89	4.0690914252197323E-4
90-91	2.4368570117135117E-4
92-93	2.5288138800800598E-5
94-95	4.230015944861191E-4
96-97	2.850662919362976E-4
98-99	0.0017218923601636043
100	0.0041978310409328985
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.1749327E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.60060996736273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.94447359381799	71.77860116722805
2	4.424938745608106	6.690561364723795
3	0.4047411354256145	0.9179603015117829
4	0.08019516625548417	0.24251213941394673
5	0.032387531556500634	0.12242585705043285
6	0.022128113024495487	0.10037393052471613
7	0.013047078470275418	0.06904569634514073
8	0.010069036011402698	0.06089802113963081
9	0.007745932317492438	0.05270374871715023
>10	0.04438294419985785	0.6454152892041193
>50	0.00670669376986273	0.3587882211798195
>100	0.006513486616513391	0.9128459351101552
>500	7.198959564865417E-4	0.38604149392261733
>1k	9.663866862111293E-4	1.5671068813118603
>5k	2.5065013436946314E-4	1.4189099467956572
>10k+	7.336101493740295E-4	14.675810005821162
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70085	0.3222398559734745	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56715	0.2607666894704374	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54713	0.251561806946946	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54259	0.24947438603502536	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52332	0.24061434176790847	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51407	0.23636133660595568	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49545	0.22780015216103008	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48027	0.2208206258520091	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47507	0.2184297472744789	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47400	0.2179377780287179	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45032	0.2070500848141186	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44332	0.20383159442128948	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43979	0.20220855569461987	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43427	0.19967054612770319	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42178	0.19392783969821228	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41494	0.19078291480007634	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41084	0.1888977989985621	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40033	0.18406546556590003	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39834	0.1831504947256529	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38461	0.1768376557122894	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37806	0.17382606827328495	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37291	0.17145817891284637	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37174	0.17092023123290206	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36714	0.16880522326047145	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36422	0.16746265298231985	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35951	0.16529706873228767	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35827	0.16472693614841508	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35687	0.16408323806984923	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35521	0.16331999606240688	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35391	0.16272227641802434	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34664	0.15937964425290033	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33440	0.15375188390886763	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33106	0.15221620420714627	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32588	0.1498345213164527	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32342	0.14870345183554415	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32233	0.14820228690294646	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32027	0.147255131158771	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31752	0.145990724218731	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31528	0.14496080729302566	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31522	0.1449332202325157	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30400	0.13977443991715238	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30356	0.139572134806746	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30317	0.13939281891343122	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29711	0.1366065258019248	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29620	0.13618812205085704	No Hit
CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29408	0.1352133792461716	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29251	0.1344915178294942	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29218	0.1343397889966894	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29210	0.1343030062493428	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29171	0.13412369035602803	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29058	0.13360413404975702	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29028	0.13346619874720722	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29027	0.1334616009037889	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27608	0.12693726109318232	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27244	0.12526364608891116	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26818	0.12330496479270371	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25361	0.1166059069322007	No Hit
CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25143	0.11560357706700533	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25023	0.11505183585680606	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24965	0.11478516093854306	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24127	0.11093216815398473	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23835	0.10958959787583313	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23825	0.10954361944164985	No Hit
CATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23397	0.10757574245860574	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23060	0.10602626922662939	No Hit
TTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22215	0.10214109153814277	No Hit
ACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21792	0.10019620377219028	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002262138961817071	4.597843418327381E-6	0.0	0.0	4.597843418327381E-6
2	0.005710521525562607	4.597843418327381E-6	0.0	1.3793530254982143E-5	4.597843418327381E-6
3	0.010248592979451732	4.597843418327381E-6	0.0	2.7587060509964286E-5	4.597843418327381E-6
4	0.01841896073381949	4.597843418327381E-6	0.0	2.7587060509964286E-5	4.597843418327381E-6
5	0.03162856487467405	4.597843418327381E-6	0.0	2.7587060509964286E-5	4.597843418327381E-6
6	0.05762936940531539	4.597843418327381E-6	0.0	2.7587060509964286E-5	4.597843418327381E-6
7	0.08939586958253927	4.597843418327381E-6	0.0	2.7587060509964286E-5	4.597843418327381E-6
8	0.21979530676972212	4.597843418327381E-6	0.0	2.7587060509964286E-5	4.597843418327381E-6
9	0.7122197390291662	4.597843418327381E-6	0.0	2.7587060509964286E-5	4.597843418327381E-6
10-11	1.6961237467255883	4.597843418327381E-6	0.0	2.7587060509964286E-5	4.597843418327381E-6
12-13	2.806183382134077	4.597843418327381E-6	0.0	2.7587060509964286E-5	4.597843418327381E-6
14-15	4.035607630525763	4.597843418327381E-6	0.0	3.448382563745535E-5	4.597843418327381E-6
16-17	5.299927211540845	4.597843418327381E-6	0.0	3.6782747346619046E-5	4.597843418327381E-6
18-19	6.651927666543429	4.597843418327381E-6	0.0	4.367951247411012E-5	4.597843418327381E-6
20-21	8.469850584342218	4.597843418327381E-6	0.0	4.5978434183273806E-5	4.597843418327381E-6
22-23	10.637496967147534	4.597843418327381E-6	0.0	4.8277355892437494E-5	4.597843418327381E-6
24-25	13.090421142686392	4.597843418327381E-6	0.0	5.517412101992857E-5	4.597843418327381E-6
26-27	15.666854887050068	4.597843418327381E-6	0.0	5.517412101992857E-5	4.597843418327381E-6
28-29	18.435924477111406	4.597843418327381E-6	0.0	5.517412101992857E-5	4.597843418327381E-6
30-31	21.420476596816076	4.597843418327381E-6	0.0	5.977196443825595E-5	4.597843418327381E-6
32-33	24.826869355543735	4.597843418327381E-6	0.0	5.977196443825595E-5	4.597843418327381E-6
34-35	28.019726771315728	4.597843418327381E-6	4.597843418327381E-6	6.207088614741964E-5	4.597843418327381E-6
36-37	30.453887147864393	4.597843418327381E-6	4.597843418327381E-6	6.436980785658333E-5	4.597843418327381E-6
38-39	32.22879724048473	4.597843418327381E-6	4.597843418327381E-6	6.89676512749107E-5	4.597843418327381E-6
40-41	33.820908573400914	4.597843418327381E-6	4.597843418327381E-6	7.816333811156548E-5	4.597843418327381E-6
42-43	35.10916912509523	4.597843418327381E-6	4.597843418327381E-6	1.0345147691236608E-4	4.597843418327381E-6
44-45	36.12593851754585	4.597843418327381E-6	4.597843418327381E-6	1.1724500716734821E-4	4.597843418327381E-6
46-47	36.96623808175765	4.597843418327381E-6	4.597843418327381E-6	1.2873961571316665E-4	4.597843418327381E-6
48-49	37.71319682673399	4.597843418327381E-6	4.597843418327381E-6	1.425331459681488E-4	4.597843418327381E-6
50-51	38.44659423254798	4.597843418327381E-6	4.597843418327381E-6	1.7241912818727678E-4	4.597843418327381E-6
52-53	39.24817535733405	4.597843418327381E-6	4.597843418327381E-6	2.1839756237055058E-4	4.597843418327381E-6
54-55	40.01739686014193	4.597843418327381E-6	4.597843418327381E-6	2.643759965538244E-4	4.597843418327381E-6
56-57	40.7925380863509	4.597843418327381E-6	4.597843418327381E-6	2.873652136454613E-4	4.597843418327381E-6
58-59	41.52448947040982	4.597843418327381E-6	4.597843418327381E-6	2.9426197877295237E-4	4.597843418327381E-6
60-61	42.2367138072824	4.597843418327381E-6	4.597843418327381E-6	2.9426197877295237E-4	4.597843418327381E-6
62-63	42.946039203879735	4.597843418327381E-6	4.597843418327381E-6	3.0115874390044346E-4	4.597843418327381E-6
64-65	43.63344438198018	4.597843418327381E-6	4.597843418327381E-6	3.379414912470625E-4	4.597843418327381E-6
66-67	44.244573636692294	4.597843418327381E-6	4.597843418327381E-6	3.8851776884866367E-4	4.597843418327381E-6
68-69	44.769824372036894	4.597843418327381E-6	4.597843418327381E-6	4.1610482935862793E-4	4.597843418327381E-6
70-71	45.254763515211295	4.597843418327381E-6	6.896765127491072E-6	4.3219728132277383E-4	4.597843418327381E-6
72-73	45.72356192906567	4.597843418327381E-6	9.195686836654762E-6	4.413929681594286E-4	4.597843418327381E-6
74-75	46.16531582793344	4.597843418327381E-6	9.195686836654762E-6	4.551864984144107E-4	4.597843418327381E-6
76-77	46.58314944641735	4.597843418327381E-6	9.195686836654762E-6	4.7127895037855656E-4	4.597843418327381E-6
78-79	46.97225573922356	4.597843418327381E-6	9.195686836654762E-6	5.49442288490122E-4	4.597843418327381E-6
80-81	47.34532935203006	9.195686836654762E-6	9.195686836654762E-6	6.413991568566697E-4	4.597843418327381E-6
82-83	47.720166237787495	9.195686836654762E-6	9.195686836654762E-6	6.712851390757976E-4	4.597843418327381E-6
84-85	48.09442379527422	9.195686836654762E-6	9.195686836654762E-6	6.712851390757976E-4	4.597843418327381E-6
86-87	48.47121246556273	9.195686836654762E-6	9.195686836654762E-6	6.804808259124524E-4	4.597843418327381E-6
88	48.755099410662226	9.195686836654762E-6	9.195686836654762E-6	6.896765127491071E-4	4.597843418327381E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	5040	0.0	59.9926	1
TGCCCTA	20340	0.0	53.721428	6
TGCTCTA	18370	0.0	52.958633	6
GCCCTAC	49400	0.0	50.413	7
GCATTTT	3140	0.0	50.16865	1
GACTTTT	4190	0.0	49.605003	1
CCCCTAC	124080	0.0	48.997486	7
CTCCCTA	42735	0.0	48.113823	6
GCCCCTA	13420	0.0	47.8731	6
GCTTTCT	4925	0.0	47.357925	1
GCCACAC	4775	0.0	47.24365	7
CCCCCTA	67235	0.0	46.903217	6
TCCCCTA	37715	0.0	46.281063	6
ATGCCCT	6385	0.0	46.155357	5
CACCCTA	24285	0.0	45.30424	6
CACTTTT	7410	0.0	45.05649	1
CGCCCTA	19365	0.0	44.607056	6
CCACACT	26300	0.0	44.442204	8
CCGCCTA	7560	0.0	44.386612	6
TTGCCCT	9020	0.0	43.927505	5
>>END_MODULE