##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248465_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18065844
Sequences flagged as poor quality	0
Sequence length	100
%GC	28
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.948098522272193	31.0	28.0	33.0	22.0	34.0
2	29.464418103023583	31.0	29.0	33.0	23.0	34.0
3	29.778559418535885	31.0	30.0	34.0	25.0	34.0
4	33.155182176930126	35.0	33.0	37.0	27.0	37.0
5	32.93999317164479	35.0	32.0	37.0	26.0	37.0
6	32.76255944643383	35.0	32.0	37.0	25.0	37.0
7	32.861810441848164	35.0	32.0	37.0	26.0	37.0
8	32.81204753013477	35.0	32.0	37.0	26.0	37.0
9	33.988374913455466	37.0	32.0	39.0	25.0	39.0
10-11	34.222366915157686	37.0	33.0	39.0	27.0	39.0
12-13	34.15858788551479	37.0	32.5	39.0	27.0	39.0
14-15	34.81348737429593	37.0	32.0	40.0	27.0	41.0
16-17	34.61093104756135	37.0	32.0	40.0	27.0	41.0
18-19	34.14769932697304	36.5	32.0	39.0	26.0	41.0
20-21	33.83415366589017	35.5	32.0	39.0	25.0	40.5
22-23	33.34087006950796	35.0	31.5	38.5	25.0	40.0
24-25	32.906978910035974	35.0	30.5	38.0	24.5	40.0
26-27	32.428972014814256	35.0	30.0	38.0	23.0	40.0
28-29	32.25601012606995	35.0	30.0	37.5	23.0	40.0
30-31	32.10681169947	35.0	30.0	37.0	22.5	40.0
32-33	31.9063537524181	34.5	30.0	37.0	22.0	40.0
34-35	31.75537218189197	34.0	28.5	37.0	22.0	40.0
36-37	31.534216917847846	34.0	28.0	37.0	21.5	40.0
38-39	31.317837212587467	34.0	27.5	37.0	20.5	40.0
40-41	31.050931774900747	33.0	26.5	37.0	20.0	39.0
42-43	30.80660264198008	33.0	26.0	37.0	20.0	39.0
44-45	30.549469097596546	33.0	25.5	36.0	19.5	39.0
46-47	30.415665993794697	33.0	25.0	36.0	20.0	39.0
48-49	30.219170219780487	33.0	25.5	35.0	18.5	39.0
50-51	30.009178342290568	33.0	26.0	35.0	18.0	39.0
52-53	29.73770021483635	33.0	26.0	35.0	18.0	39.0
54-55	29.539554863863543	32.0	26.0	35.0	17.0	38.0
56-57	29.30884812245694	32.0	26.0	35.0	15.0	38.0
58-59	29.016030250233534	31.0	26.0	35.0	15.0	37.0
60-61	28.74588776477866	31.0	26.0	35.0	15.0	37.0
62-63	28.693917344797175	31.0	25.5	35.0	15.5	36.5
64-65	28.954270832848994	31.0	26.0	35.0	17.5	36.0
66-67	29.41900796331464	32.0	26.0	35.0	20.0	36.0
68-69	29.444285249003592	32.0	26.0	35.0	20.0	35.5
70-71	29.778236765467476	32.0	27.5	35.0	20.0	35.0
72-73	29.795038609876187	33.0	27.5	35.0	20.0	35.0
74-75	29.71223276366164	32.0	27.0	35.0	20.0	35.0
76-77	29.596902862661715	32.0	27.0	35.0	20.0	35.0
78-79	29.470662594008893	32.0	27.0	35.0	19.0	35.0
80-81	29.372285678986266	32.0	27.0	35.0	19.0	35.0
82-83	29.26412555095682	32.0	27.0	35.0	18.5	35.0
84-85	29.151331401953875	32.0	27.0	35.0	18.0	35.0
86-87	28.976493569854803	32.0	27.0	35.0	16.5	35.0
88-89	28.802864123037928	32.0	26.5	35.0	15.0	35.0
90-91	28.563333299014428	32.0	26.0	35.0	11.0	35.0
92-93	28.31648930988223	32.0	25.5	35.0	10.0	35.0
94-95	27.986890897541237	32.0	25.0	35.0	9.5	35.0
96-97	27.623670612898017	32.0	25.0	35.0	8.0	35.0
98-99	27.18397836824009	31.5	24.5	34.5	7.5	35.0
100	25.810994050430192	30.0	20.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6678048845247098
1101	2	0.605536108884678
1101	3	0.6030888986809586
1101	4	0.5695955268719359
1101	5	0.4603217321309998
1101	6	0.3136658523011562
1101	7	0.3209064723218873
1101	8	0.46614997753604825
1101	9	0.44826604010216187
1101	10-11	0.49746132857443826
1101	12-13	0.5888363680783968
1101	14-15	0.6955167666667421
1101	16-17	0.6974438494082662
1101	18-19	0.7385902318863913
1101	20-21	0.5931570397624526
1101	22-23	0.5292145250288058
1101	24-25	0.5543148697783096
1101	26-27	0.6192479525112233
1101	28-29	0.4498908691348049
1101	30-31	0.3788377787116488
1101	32-33	0.4151124420137755
1101	34-35	0.42819603654076843
1101	36-37	0.38121768194236694
1101	38-39	0.31513783378071736
1101	40-41	0.37041674979875694
1101	42-43	0.2893807056750397
1101	44-45	0.2716726351359249
1101	46-47	0.29315963733871797
1101	48-49	0.25315395461880996
1101	50-51	0.3584744337843446
1101	52-53	0.2975299542529335
1101	54-55	0.20860089105492108
1101	56-57	0.2060436484253252
1101	58-59	0.2101324170871095
1101	60-61	0.1581234217555405
1101	62-63	0.1966921526732115
1101	64-65	0.29481234825568237
1101	66-67	0.3578672326400181
1101	68-69	0.3838684497871334
1101	70-71	0.44835420517657454
1101	72-73	0.438277480549786
1101	74-75	0.3882828883902327
1101	76-77	0.36526886685392057
1101	78-79	0.37905547380165316
1101	80-81	0.3394909224600866
1101	82-83	0.24366332715218064
1101	84-85	0.2479130195672994
1101	86-87	0.2317951877042823
1101	88-89	0.19840375499455476
1101	90-91	0.25847785927080125
1101	92-93	0.24536030919217566
1101	94-95	0.2588292188947072
1101	96-97	0.18995159568025954
1101	98-99	0.1293938601471858
1101	100	0.05154278130432388
1102	1	0.423474541470096
1102	2	0.43968963349917445
1102	3	0.3894319922009295
1102	4	0.400632806587069
1102	5	0.34107298408913067
1102	6	0.271243560372632
1102	7	0.2971300686605147
1102	8	0.35905454040672424
1102	9	0.31019180681010283
1102	10-11	0.28560812082937304
1102	12-13	0.37254307518591645
1102	14-15	0.49994667652514124
1102	16-17	0.4809583717884962
1102	18-19	0.5868119765938502
1102	20-21	0.4431826458911772
1102	22-23	0.3202910645167094
1102	24-25	0.2888961889116146
1102	26-27	0.37288700508254635
1102	28-29	0.26970493208369106
1102	30-31	0.23794652413766215
1102	32-33	0.22486752534753762
1102	34-35	0.2526761245980822
1102	36-37	0.18981203066549668
1102	38-39	0.10867129493502858
1102	40-41	0.18044445188390412
1102	42-43	0.15253197267339047
1102	44-45	0.09869402163423757
1102	46-47	0.13165125844582448
1102	48-49	0.19492762581140965
1102	50-51	0.12224030877072778
1102	52-53	0.05647876946946084
1102	54-55	0.11459184388669286
1102	56-57	0.06554835492278599
1102	58-59	0.07798559848917819
1102	60-61	0.12982614745064325
1102	62-63	0.14840271306246677
1102	64-65	0.16507854690081558
1102	66-67	0.20692866749600825
1102	68-69	0.21727675540854818
1102	70-71	0.21766254686562903
1102	72-73	0.18389092963712272
1102	74-75	0.24203962406007662
1102	76-77	0.20738472302871358
1102	78-79	0.1869492543980975
1102	80-81	0.1524807472232652
1102	82-83	0.14663918594839842
1102	84-85	0.10896858069942894
1102	86-87	0.08894478458414312
1102	88-89	0.05652150417677504
1102	90-91	0.06986088531928303
1102	92-93	0.04614086330312972
1102	94-95	0.015379040079839257
1102	96-97	-0.01810298162600077
1102	98-99	0.06904146838268943
1102	100	-0.034466401615617315
1103	1	0.24652371047668353
1103	2	0.24368226678767257
1103	3	0.27755177297973077
1103	4	0.27547371126918563
1103	5	0.24502741013969143
1103	6	0.14855078511902775
1103	7	0.24785395653938025
1103	8	0.28963552134805326
1103	9	0.2978001561827597
1103	10-11	0.2394997513558863
1103	12-13	0.28400466472272257
1103	14-15	0.36820249946561034
1103	16-17	0.3286116562025043
1103	18-19	0.4177700809399312
1103	20-21	0.2909374407388299
1103	22-23	0.23484955177909228
1103	24-25	0.20007867028974857
1103	26-27	0.3315862063716182
1103	28-29	0.14925980226783508
1103	30-31	0.0950069167758798
1103	32-33	0.05071484213711486
1103	34-35	0.14165074122891497
1103	36-37	0.03820801812751995
1103	38-39	0.02085647153875314
1103	40-41	0.03650162514200872
1103	42-43	0.06556854515910615
1103	44-45	0.0414357878632039
1103	46-47	0.03552485838667252
1103	48-49	0.019465869995649143
1103	50-51	0.008262222912797768
1103	52-53	0.03963883786865807
1103	54-55	0.02859634515424858
1103	56-57	-0.03582825202493822
1103	58-59	-0.0741181319201516
1103	60-61	-0.028894006832754116
1103	62-63	-0.014649171163405583
1103	64-65	0.04993733415264501
1103	66-67	0.0757862659719919
1103	68-69	0.11940955908649187
1103	70-71	0.1540794023694012
1103	72-73	0.12408735044306951
1103	74-75	0.13882358996906774
1103	76-77	0.08664211986674175
1103	78-79	0.06701120698937402
1103	80-81	0.07525336043994457
1103	82-83	0.004470120338051942
1103	84-85	-0.034482167970409705
1103	86-87	-0.0372328624575573
1103	88-89	-0.033451074630310274
1103	90-91	0.021901717186782577
1103	92-93	0.004626077687188257
1103	94-95	0.05474296835618375
1103	96-97	0.010747922671573207
1103	98-99	-0.004372437169578802
1103	100	-0.02788829706284801
1104	1	0.23970640692269285
1104	2	0.1971679199030163
1104	3	0.20252106325569486
1104	4	0.22993419141084104
1104	5	0.21679140340464187
1104	6	0.07609068096299154
1104	7	0.16275099452431618
1104	8	0.27780247817726433
1104	9	0.15574695048113796
1104	10-11	0.1417558437912234
1104	12-13	0.17530902892666944
1104	14-15	0.31221971901025825
1104	16-17	0.3258103958576939
1104	18-19	0.3595659019592219
1104	20-21	0.23420498273534918
1104	22-23	0.17733407013687952
1104	24-25	0.21307513849445314
1104	26-27	0.2975215826728359
1104	28-29	0.0843228629952506
1104	30-31	0.09273364880709067
1104	32-33	0.06915986282621134
1104	34-35	0.07653313862903843
1104	36-37	0.06595580415518043
1104	38-39	0.014431934659697276
1104	40-41	-0.005856523878229325
1104	42-43	0.03195224103604488
1104	44-45	-0.04770944376454267
1104	46-47	-0.025222651575884214
1104	48-49	-0.08509783211660604
1104	50-51	-0.03383725428177087
1104	52-53	-0.046698988599448654
1104	54-55	-0.04073422719110198
1104	56-57	-0.04963933674867249
1104	58-59	-0.10033215220045477
1104	60-61	-0.06702249005523697
1104	62-63	-0.006185532972200036
1104	64-65	0.10756474828809459
1104	66-67	0.0627172906680542
1104	68-69	0.08276970034632214
1104	70-71	0.023461688490581167
1104	72-73	0.014248923369422073
1104	74-75	0.03941769677797069
1104	76-77	0.09955303874115273
1104	78-79	0.050765725069961576
1104	80-81	0.021054107225161545
1104	82-83	-0.004057681605640795
1104	84-85	-0.06757423960478803
1104	86-87	-0.052568301121901584
1104	88-89	-0.08900795445234522
1104	90-91	-0.05152779682538977
1104	92-93	-0.07022155414594167
1104	94-95	-0.015190192030754446
1104	96-97	0.002030904261324906
1104	98-99	-0.014051462645962687
1104	100	-0.0848589397564794
1105	1	0.22017876721956853
1105	2	0.23948611020841426
1105	3	0.21802014227284872
1105	4	0.22818053272641237
1105	5	0.19037076520278617
1105	6	0.0951346121776595
1105	7	0.1047975866669475
1105	8	0.2336347175954998
1105	9	0.2093643370756979
1105	10-11	0.22387819896311356
1105	12-13	0.219795656226502
1105	14-15	0.2766621671530345
1105	16-17	0.25955786505453915
1105	18-19	0.33807281201373485
1105	20-21	0.25097617352720647
1105	22-23	0.17170244769586418
1105	24-25	0.11617544242030675
1105	26-27	0.21684174311177173
1105	28-29	0.0913855219851385
1105	30-31	0.017155302458355948
1105	32-33	0.06151348233703047
1105	34-35	0.029502283315267164
1105	36-37	-0.029008578771787086
1105	38-39	-0.03980578336010865
1105	40-41	-0.0703397783116344
1105	42-43	-0.10165529045605126
1105	44-45	-0.05535479964308365
1105	46-47	-0.037777288324310376
1105	48-49	-0.08351761463985241
1105	50-51	-0.09917287315311185
1105	52-53	-0.1184174689257631
1105	54-55	-0.11472705368441893
1105	56-57	-0.12044090142272168
1105	58-59	-0.0833025623440733
1105	60-61	-0.10992241216578336
1105	62-63	-0.09566382611737367
1105	64-65	0.041557998287142794
1105	66-67	0.0026872061353095944
1105	68-69	0.009896304504867004
1105	70-71	-0.022766560364917865
1105	72-73	-0.03921125357626565
1105	74-75	0.008466586006878885
1105	76-77	-0.007815017913049616
1105	78-79	-0.0065498317851897525
1105	80-81	-0.027152329933365138
1105	82-83	-0.0455339857352719
1105	84-85	-0.037092166862198184
1105	86-87	-0.01728883841396822
1105	88-89	-0.0017105818838025755
1105	90-91	-0.017704524597068172
1105	92-93	0.014744538281004083
1105	94-95	0.040787099811627314
1105	96-97	0.03508664341341472
1105	98-99	0.01785542680501706
1105	100	0.042857828373279006
1106	1	0.020873583960781872
1106	2	0.12935695628317134
1106	3	0.13284669664707494
1106	4	0.09444257596403816
1106	5	0.15360583143660733
1106	6	0.03104845510453913
1106	7	0.05149164985990495
1106	8	0.18702038359513296
1106	9	0.122271349919437
1106	10-11	0.0908149487404657
1106	12-13	0.19251612080125113
1106	14-15	0.2454162387638803
1106	16-17	0.14614113270040008
1106	18-19	0.2129239236787157
1106	20-21	0.13012972033317283
1106	22-23	0.10746949405715611
1106	24-25	0.06644729167538799
1106	26-27	0.1678277684565117
1106	28-29	-0.001030802658519292
1106	30-31	-0.0034710972361295944
1106	32-33	-0.021869506040196995
1106	34-35	-0.05438997689610403
1106	36-37	-0.07519303186722937
1106	38-39	-0.05415840425472496
1106	40-41	-0.0519829088863375
1106	42-43	-0.057502550004379316
1106	44-45	-0.11253932629577434
1106	46-47	-0.13098416540428914
1106	48-49	-0.07780193603498375
1106	50-51	-0.09160019450703061
1106	52-53	-0.07267750707789133
1106	54-55	-0.06571257779216211
1106	56-57	-0.06430721313854804
1106	58-59	-0.1128850132872472
1106	60-61	-0.06151533026375233
1106	62-63	-0.017495811367336245
1106	64-65	0.037762552203773225
1106	66-67	0.01691239969431635
1106	68-69	-0.00960640775086219
1106	70-71	0.05367368828088459
1106	72-73	0.05469454193893597
1106	74-75	0.03965621831984478
1106	76-77	0.11370190708408145
1106	78-79	0.11174948388866923
1106	80-81	0.0714187870057188
1106	82-83	0.06454858751904524
1106	84-85	0.03743810958360072
1106	86-87	0.08009188431757508
1106	88-89	0.04432712548580753
1106	90-91	0.052855025332892325
1106	92-93	0.008692908437780034
1106	94-95	0.035685054165593755
1106	96-97	0.04737136092040117
1106	98-99	0.03577291330387666
1106	100	0.03212155227147662
1107	1	0.03421358684423126
1107	2	0.03414040044525635
1107	3	0.07471334096008064
1107	4	0.05528284572376663
1107	5	0.07284422319903427
1107	6	0.00850617828175615
1107	7	0.014708256832349775
1107	8	0.18407700496154433
1107	9	0.1148572362046707
1107	10-11	0.052596067793864165
1107	12-13	0.12604332893770476
1107	14-15	0.18069431093518062
1107	16-17	0.17241274857655497
1107	18-19	0.19742554256502842
1107	20-21	0.11406129679686927
1107	22-23	0.03406359058550379
1107	24-25	0.028234991921443964
1107	26-27	0.19672333598192893
1107	28-29	-0.01130390410103388
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2216	7	0.46414790788193727
2216	8	0.30829092282911574
2216	9	0.44372003367001867
2216	10-11	0.5676845368237693
2216	12-13	0.5499157450192556
2216	14-15	0.614923537024552
2216	16-17	0.567297990112472
2216	18-19	0.6844099432150017
2216	20-21	0.5850915929522671
2216	22-23	0.8236139648150385
2216	24-25	0.8766445869366493
2216	26-27	0.9771629504128825
2216	28-29	1.0479793626050764
2216	30-31	1.141656393211342
2216	32-33	1.2353031460244885
2216	34-35	1.1530704546955661
2216	36-37	1.2072155524029142
2216	38-39	1.2337265077135626
2216	40-41	1.3137024294177735
2216	42-43	1.2770804444286945
2216	44-45	1.293009684007366
2216	46-47	1.2105989770409273
2216	48-49	1.2018890663129866
2216	50-51	1.189750171460183
2216	52-53	1.201902027107426
2216	54-55	1.1841835268954917
2216	56-57	1.1467881533497852
2216	58-59	1.1883431829175066
2216	60-61	1.1532375939197799
2216	62-63	1.0067170248759822
2216	64-65	0.9125303207943318
2216	66-67	0.9536194332263079
2216	68-69	0.9256462472397935
2216	70-71	0.8174577205584193
2216	72-73	0.7748374741611457
2216	74-75	0.8014800852070856
2216	76-77	0.8223799251286223
2216	78-79	0.7724945165991421
2216	80-81	0.7830569795853748
2216	82-83	0.7047912624156645
2216	84-85	0.6728719260819105
2216	86-87	0.6388773520236057
2216	88-89	0.5488553608476074
2216	90-91	0.5556655813262559
2216	92-93	0.4873917703799755
2216	94-95	0.42195590175483133
2216	96-97	0.43462264776353976
2216	98-99	0.3963948131551227
2216	100	0.6859913258012398
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	310.0
6	3569.0
7	9537.0
8	13796.0
9	18911.0
10	27735.0
11	38095.0
12	46822.0
13	56118.0
14	67049.0
15	81442.0
16	98327.0
17	119382.0
18	145874.0
19	173689.0
20	201649.0
21	231261.0
22	263495.0
23	309938.0
24	428856.0
25	464949.0
26	544292.0
27	840478.0
28	762948.0
29	1178665.0
30	1194370.0
31	1449881.0
32	1806212.0
33	2488771.0
34	1948067.0
35	2698222.0
36	290484.0
37	56005.0
38	6317.0
39	322.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.931054986397195	30.904010177485485	26.72837603999921	26.436558796118113
2	13.409076265615456	33.6181945554894	28.7856594542736	24.187069724621544
3	11.754005135004203	35.91635093210334	26.289917395335987	26.039726537556472
4	10.38741855068318	35.96041168534907	26.270691264993218	27.381478498974527
5	10.331866527597166	35.42935605296354	27.40589158944473	26.832885829994563
6	10.280333429204857	32.60509168572473	28.663404820721354	28.451170064349057
7	13.680689371611976	37.74330166916088	23.8825985655583	24.69341039366885
8	12.521961332113795	34.77617209580687	21.520345243764975	31.18152132831436
9	9.922248858121437	34.16044664174007	22.69044833997238	33.226856160166115
10-11	13.553756469944055	49.588098402709555	21.133100673292653	15.725044454053737
12-13	13.301594055543006	50.24557537143931	20.88131419972563	15.571516373292058
14-15	14.189630442950795	49.165328229337085	20.663496263999622	15.981545063712495
16-17	14.030152701418212	48.96418622899655	21.336130213456954	15.669530856128283
18-19	14.358424106839404	48.04249665833492	22.299539396000544	15.299539838825133
20-21	13.973630009655238	48.109642181578174	22.41859068931147	15.498137119455121
22-23	14.469176139147017	48.84852862102967	22.67991439314038	14.002380846682927
24-25	14.61856895404864	48.68815538469024	22.24727013513529	14.446005526125827
26-27	15.894701535516578	48.753063071986894	21.55374493459593	13.7984904579006
28-29	15.59199227005392	49.526022697860114	21.78516818810126	13.096816843984705
30-31	15.948861841162804	49.563820544448404	21.5711040126329	12.916213601755889
32-33	16.303201776789393	49.596442878616685	21.105770092999805	12.994585251594112
34-35	16.389926205495854	49.59529430233096	21.062187850177384	12.9525916419958
36-37	16.99811257088238	49.372559067818806	20.664821970122183	12.96450639117663
38-39	17.382415125471027	49.602822320396434	20.675424851448955	12.339337702683583
40-41	17.599669564097333	49.86797869027188	20.623025779574466	11.909325966056313
42-43	17.874831539698512	49.88519762918159	20.83516344586235	11.40480738525755
44-45	17.98898938366376	49.63677575525563	20.82996209918201	11.544272761898606
46-47	17.905427182038807	49.31251981818568	21.181679620106365	11.600373379669152
48-49	17.82961349010252	49.3553509489942	21.58115836035833	11.233877200544951
50-51	18.027795942048304	49.26680818053366	21.85021470218501	10.855181175233025
52-53	18.238105467871055	49.273075715315365	22.01427854764375	10.474540269169836
54-55	18.245892898094255	49.42276144243714	21.92897911589059	10.402366543578015
56-57	18.031216037425768	49.564155382295496	21.633486554349364	10.771142025929375
58-59	17.736578777485406	49.34744375952679	21.76767891330806	11.148298549679748
60-61	17.75821268023791	48.89474026234257	22.159219353383104	11.187827704036412
62-63	17.81616003194537	48.153163956976705	22.513596514704574	11.517079496373348
64-65	17.565731077955387	47.974544646490436	22.595971460259513	11.863752815294667
66-67	16.95829580849129	47.76932227229225	23.085806836228212	12.186575082988252
68-69	16.622604107879802	47.72337265156636	22.878787237193563	12.775236003360273
70-71	16.114867739133704	47.885919055775254	23.01187460280391	12.98733860228713
72-73	16.138635693044094	48.283601696588754	22.96691526834393	12.610847342023224
74-75	15.898128455358131	48.8212339493767	22.76394054642522	12.516697048839948
76-77	15.186603791700238	49.69791879750174	22.780751217667156	12.33472619313087
78-79	14.558946764251967	50.686275687422885	22.414972706010435	12.33980484231471
80-81	13.882257211891075	52.08800126206364	21.91185205071503	12.117889475330253
82-83	13.280479652001546	53.2831809188708	21.765526991288276	11.67081243783938
84-85	12.619143934995813	54.60754184872595	21.59517864504938	11.178135571228855
86-87	12.12829990615229	55.73308823179377	21.258942074916757	10.879669787137185
88-89	11.801607231142443	56.63376485120063	21.06041120059424	10.50421671706268
90-91	11.739343187876964	57.43829101311676	20.607779273574295	10.214586525431985
92-93	11.834590303921708	57.97832089343318	20.057722310230876	10.12936649241424
94-95	12.111438838827773	58.072765258447376	19.597611277607346	10.218184625117498
96-97	12.454636957412399	57.80110929091408	19.401251705979206	10.343002045694323
98-99	12.85353104192655	57.3079155443739	19.23485915330209	10.603694260397457
100	13.657946864161886	56.26366375625257	19.198453855770516	10.879935523815034
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1523530.0
1	1513906.0
2	959412.0
3	283834.0
4	155848.5
5	155911.5
6	136641.5
7	99376.5
8	64121.5
9	44538.5
10	40574.5
11	46012.0
12	57643.5
13	75267.0
14	100361.5
15	131849.0
16	166824.0
17	202995.5
18	239711.5
19	275366.5
20	305092.5
21	329456.5
22	349646.0
23	364706.5
24	375227.0
25	379844.0
26	377206.0
27	368644.5
28	353799.5
29	333270.0
30	310845.0
31	287601.5
32	264314.0
33	242699.0
34	223921.0
35	207478.0
36	194391.5
37	184704.5
38	178864.5
39	177120.0
40	180204.0
41	190607.5
42	210092.5
43	242332.5
44	289125.0
45	350016.5
46	417954.5
47	479946.0
48	522041.5
49	535046.0
50	520507.0
51	486706.0
52	442792.0
53	393675.0
54	340995.5
55	285769.0
56	228301.0
57	172145.5
58	122669.0
59	83460.5
60	55533.0
61	37273.5
62	25876.0
63	18895.5
64	14755.0
65	11980.0
66	9797.5
67	8278.5
68	7061.0
69	5978.5
70	5128.5
71	4433.5
72	3876.0
73	3378.5
74	3001.0
75	2632.5
76	2370.5
77	2208.5
78	2003.0
79	1848.5
80	1724.0
81	1657.5
82	1583.0
83	1447.5
84	1393.0
85	1364.5
86	1327.0
87	1272.0
88	1177.5
89	1126.5
90	1094.0
91	1043.0
92	1015.0
93	980.0
94	929.0
95	904.0
96	869.0
97	799.5
98	755.5
99	726.0
100	761.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.705011290920037E-4
2	4.8710705129525084E-4
3	1.9927106643896628E-4
4	4.428245920865917E-5
5	3.155125218616966E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.0444190705953177E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.7676537005411978E-5
22-23	1.1070614802164792E-5
24-25	1.273120702248951E-4
26-27	1.9096810533734265E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	5.812072771136516E-5
42-43	8.302961101623595E-5
44-45	3.874715180757678E-5
46-47	2.79533023754661E-4
48-49	3.5979498107035574E-5
50-51	3.0444190705953177E-5
52-53	5.535307401082396E-6
54-55	1.660592220324719E-5
56-57	1.1070614802164792E-5
58-59	3.5979498107035574E-5
60-61	0.0
62-63	5.535307401082396E-6
64-65	9.686787951894194E-5
66-67	1.8820045163680146E-4
68-69	2.906036385568258E-4
70-71	8.026195731569474E-4
72-73	3.5149201996873214E-4
74-75	8.38599071263983E-4
76-77	0.0014087357335754698
78-79	8.026195731569474E-4
80-81	0.0010738496358099847
82-83	0.0013644532743668106
84-85	0.0016605922203247188
86-87	0.0022473348048394526
88-89	0.0023663439139627244
90-91	0.002236264190037288
92-93	0.0021338610031172637
94-95	0.0011264350561202678
96-97	0.0018792368626674738
98-99	0.001846025018260979
100	0.0014336446168803406
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.8065844E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.27397256650963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.92620232888272	70.50546147618377
2	4.3041849870953754	6.39377835305409
3	0.40820174190595504	0.9095629493977303
4	0.09662141343092515	0.2870582484202368
5	0.05436056770986153	0.20187876573910724
6	0.038064991995765865	0.16963429027427535
7	0.026541005274438232	0.13799141283488486
8	0.018837798859934828	0.11193265245889807
9	0.01655317150224819	0.11065228254420398
>10	0.09500175878753683	1.321382251185155
>50	0.00842268273036092	0.42837098838550325
>100	0.004942364008287737	0.7292517956792705
>500	7.368945815009621E-4	0.38035343658725845
>1k	7.354767537595414E-4	1.0998505075901726
>5k	1.951041584102815E-4	0.9946454172025174
>10k+	3.977123229132662E-4	16.218195172463066
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	669584	3.7063532708463547	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	207125	1.1465005454491912	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	173601	0.9609349001353051	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	163283	0.903821598370937	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115928	0.64169711639268	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93309	0.5164939982875972	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86916	0.48110677807247754	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	79784	0.4416289656879579	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	74010	0.4096681007541081	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70297	0.3891155043738892	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70097	0.3880084428936727	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62832	0.34779443462480913	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59719	0.3305630226852396	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59505	0.329378466901408	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58145	0.3218504488359359	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49859	0.27598489171056717	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48659	0.2693425228292683	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46862	0.25939557542952324	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45250	0.25047265989897843	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38646	0.21391748982223027	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37753	0.20897446031306371	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37136	0.20555917564659587	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34880	0.19307152214975398	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31141	0.1723750077771069	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27359	0.15144047518621326	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27034	0.14964150028086148	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25254	0.13978865310693483	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24737	0.13692689918057524	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23935	0.13248758264490715	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22479	0.12442817506893118	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20786	0.11505689963889867	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20354	0.11266564684163109	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19796	0.10957694531182711	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18948	0.10488300463570924	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18633	0.1031393828043683	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0019484282051810034	0.0	0.0	0.0	0.0
2	0.004859999898150343	0.0	0.0	1.1070614802164792E-5	5.535307401082396E-6
3	0.009160933748791365	0.0	0.0	4.981776660974156E-5	5.535307401082396E-6
4	0.016439862981214717	0.0	0.0	4.981776660974156E-5	5.535307401082396E-6
5	0.02642555753276736	0.0	0.0	4.981776660974156E-5	5.535307401082396E-6
6	0.04525113800384859	0.0	0.0	5.535307401082396E-5	5.535307401082396E-6
7	0.06712667285292621	0.0	0.0	5.535307401082396E-5	5.535307401082396E-6
8	0.1334119789808879	0.0	0.0	5.535307401082396E-5	5.535307401082396E-6
9	0.3631438420480106	0.0	0.0	5.535307401082396E-5	5.535307401082396E-6
10-11	0.8766847538371304	0.0	0.0	5.535307401082396E-5	5.535307401082396E-6
12-13	1.5626975412828763	0.0	0.0	5.535307401082396E-5	5.535307401082396E-6
14-15	2.401133320978527	0.0	0.0	5.535307401082396E-5	5.535307401082396E-6
16-17	3.3544073556707343	0.0	0.0	5.535307401082396E-5	5.535307401082396E-6
18-19	4.415957538435514	0.0	0.0	6.642368881298875E-5	5.535307401082396E-6
20-21	5.849649205428763	2.767653700541198E-6	0.0	6.642368881298875E-5	5.535307401082396E-6
22-23	7.459784331138916	5.535307401082396E-6	0.0	6.642368881298875E-5	5.535307401082396E-6
24-25	9.077552645755162	5.535307401082396E-6	0.0	6.642368881298875E-5	5.535307401082396E-6
26-27	10.688224696283218	5.535307401082396E-6	0.0	6.642368881298875E-5	5.535307401082396E-6
28-29	12.394411243670653	5.535307401082396E-6	0.0	6.642368881298875E-5	5.535307401082396E-6
30-31	14.25444612496377	5.535307401082396E-6	0.0	6.642368881298875E-5	5.535307401082396E-6
32-33	16.348428005909938	5.535307401082396E-6	0.0	8.302961101623595E-5	5.535307401082396E-6
34-35	18.242427533416098	5.535307401082396E-6	0.0	8.302961101623595E-5	5.535307401082396E-6
36-37	19.74374405092837	5.535307401082396E-6	0.0	9.133257211785953E-5	5.535307401082396E-6
38-39	20.865936293925706	5.535307401082396E-6	0.0	1.0240318692002433E-4	5.535307401082396E-6
40-41	21.778415113071937	5.535307401082396E-6	0.0	1.1347380172218913E-4	5.535307401082396E-6
42-43	22.48046368605862	8.302961101623594E-6	0.0	1.411503387276011E-4	5.535307401082396E-6
44-45	23.030341123282145	1.1070614802164792E-5	0.0	1.6605922203247187E-4	5.535307401082396E-6
46-47	23.481324758477932	1.1070614802164792E-5	0.0	1.8266514423571909E-4	5.535307401082396E-6
48-49	23.879875194316966	1.1070614802164792E-5	0.0	1.9373575903788387E-4	5.535307401082396E-6
50-51	24.27915075542554	1.1070614802164792E-5	0.0	2.2141229604329585E-4	5.535307401082396E-6
52-53	24.709745085809445	1.1070614802164792E-5	0.0	2.6015944785087264E-4	5.535307401082396E-6
54-55	25.162234324618325	1.660592220324719E-5	0.0	2.9890659965844935E-4	5.535307401082396E-6
56-57	25.653326797242354	1.660592220324719E-5	0.0	3.2935079036440257E-4	5.535307401082396E-6
58-59	26.170291296659045	1.660592220324719E-5	0.0	3.48724366268191E-4	5.535307401082396E-6
60-61	26.70591254967108	1.660592220324719E-5	0.0	3.5425967366927336E-4	5.535307401082396E-6
62-63	27.21525216314278	1.660592220324719E-5	0.0	3.6533028847143817E-4	5.535307401082396E-6
64-65	27.689935216976302	1.660592220324719E-5	0.0	3.8747151807576774E-4	5.535307401082396E-6
66-67	28.109688647815183	1.660592220324719E-5	0.0	4.096127476800973E-4	5.535307401082396E-6
68-69	28.467009346477255	1.660592220324719E-5	0.0	4.428245920865917E-4	5.535307401082396E-6
70-71	28.780908326231533	1.660592220324719E-5	0.0	4.511275531882153E-4	5.535307401082396E-6
72-73	29.064249641478142	1.660592220324719E-5	0.0	4.5666286058929767E-4	5.535307401082396E-6
74-75	29.326520809102526	1.660592220324719E-5	0.0	4.843393975947096E-4	5.535307401082396E-6
76-77	29.576935348273793	1.937357590378839E-5	0.0	5.17551242001204E-4	5.535307401082396E-6
78-79	29.816735935503484	2.2141229604329585E-5	0.0	5.396924716055336E-4	5.535307401082396E-6
80-81	30.050525178895597	2.2141229604329585E-5	0.0	6.061161604185224E-4	5.535307401082396E-6
82-83	30.284746176265	2.2141229604329585E-5	0.0	6.254897363223108E-4	5.535307401082396E-6
84-85	30.52248209383409	2.2141229604329585E-5	0.0	6.365603511244755E-4	8.302961101623594E-6
86-87	30.76518041448825	2.2141229604329585E-5	0.0	6.42095658525558E-4	1.1070614802164792E-5
88	30.952221219224523	2.2141229604329585E-5	0.0	6.42095658525558E-4	1.1070614802164792E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGA	9075	0.0	38.013947	6
GCTTGCT	1235	0.0	37.67574	7
TTGCTGA	5480	0.0	36.364605	9
CCCGACG	10990	0.0	36.137043	8
ACCCGAC	10185	0.0	35.855316	7
GACACGC	875	0.0	34.91394	2
CCTGCTG	4695	0.0	34.135975	8
GCCTGCT	1185	0.0	34.109367	7
CTACCCG	9385	0.0	33.70345	5
TATGGCC	740	0.0	32.39157	3
TACGGCC	335	0.0	32.26834	3
CTGCTGA	5770	0.0	32.25618	9
CTTGCTG	3640	0.0	32.150784	8
TGACGCC	850	0.0	31.517338	3
CATTGCC	915	0.0	30.305712	3
CCGACGC	13125	0.0	29.864845	9
ATGGCCT	565	0.0	28.282907	4
TACGTGC	370	0.0	27.94567	2
TTCGCGA	640	0.0	27.905975	5
ATGTGCC	590	0.0	27.881079	3
>>END_MODULE