##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248465_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18065844
Sequences flagged as poor quality	0
Sequence length	100
%GC	29
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.426643892197895	34.0	31.0	34.0	31.0	34.0
2	32.6567206049161	34.0	31.0	34.0	31.0	34.0
3	32.76443026962925	34.0	31.0	34.0	31.0	34.0
4	36.23814580708214	37.0	37.0	37.0	35.0	37.0
5	36.11869387336678	37.0	35.0	37.0	35.0	37.0
6	36.025678512445914	37.0	35.0	37.0	35.0	37.0
7	36.05245633694169	37.0	35.0	37.0	35.0	37.0
8	35.93623397832949	37.0	35.0	37.0	35.0	37.0
9	37.620642190865816	39.0	37.0	39.0	35.0	39.0
10-11	37.763658066570265	39.0	38.0	39.0	35.0	39.0
12-13	37.790131144717066	39.0	38.0	39.0	35.0	39.0
14-15	39.24701157056377	41.0	39.0	41.0	36.0	41.0
16-17	39.18145822580999	41.0	39.0	41.0	36.0	41.0
18-19	38.926433412133974	40.5	38.5	41.0	35.5	41.0
20-21	38.51286117604027	40.0	37.5	41.0	35.0	41.0
22-23	38.068830385117906	39.5	36.0	41.0	34.0	41.0
24-25	37.689226669952426	39.0	35.0	41.0	34.0	41.0
26-27	37.49702468924231	39.0	35.0	41.0	33.0	41.0
28-29	37.35737331175892	39.0	35.0	41.0	33.0	41.0
30-31	37.29045559676038	39.0	35.0	41.0	33.0	41.0
32-33	37.220705437288174	39.0	35.0	41.0	33.0	41.0
34-35	37.16246736105991	39.0	35.0	41.0	33.0	41.0
36-37	37.146973814231984	39.0	35.0	41.0	33.0	41.0
38-39	37.05126223275259	39.0	35.0	41.0	33.0	41.0
40-41	36.946991709880805	39.0	35.0	41.0	33.0	41.0
42-43	36.823261066574034	38.5	35.0	41.0	33.0	41.0
44-45	36.69127357127627	38.0	35.0	41.0	33.0	41.0
46-47	36.50290122066813	38.0	35.0	40.0	32.0	41.0
48-49	36.25528621856803	37.0	35.0	40.0	32.0	41.0
50-51	36.000791272192984	36.5	35.0	40.0	31.0	41.0
52-53	35.733565091118905	36.0	35.0	39.5	31.0	41.0
54-55	35.503059198341354	35.0	35.0	39.0	31.0	41.0
56-57	35.22197866869658	35.0	35.0	39.0	31.0	41.0
58-59	34.93621133892222	35.0	35.0	38.5	30.5	40.5
60-61	34.690466578810266	35.0	34.0	37.5	30.0	40.0
62-63	34.443765594344775	35.0	34.0	37.0	30.0	40.0
64-65	34.234564463193635	35.0	34.0	36.0	29.5	40.0
66-67	34.03919645270932	35.0	34.0	36.0	29.0	39.0
68-69	33.84909451227411	35.0	34.0	35.5	29.0	39.0
70-71	33.67822770970457	35.0	34.0	35.0	29.0	39.0
72-73	33.49745320506476	35.0	33.0	35.0	29.0	37.5
74-75	33.33433918171772	35.0	33.0	35.0	29.0	37.0
76-77	33.163843521509435	35.0	33.0	35.0	29.0	36.5
78-79	33.00750349665368	35.0	33.0	35.0	29.0	36.0
80-81	32.87506501218543	35.0	33.0	35.0	28.0	36.0
82-83	32.7304788528009	35.0	33.0	35.0	27.0	36.0
84-85	32.56652606432337	35.0	33.0	35.0	27.0	35.0
86-87	32.38998197371792	35.0	33.0	35.0	27.0	35.0
88-89	32.24206270130529	35.0	33.0	35.0	26.0	35.0
90-91	32.10735045093935	35.0	32.0	35.0	25.0	35.0
92-93	31.937563448461084	35.0	32.0	35.0	25.0	35.0
94-95	31.718616938129212	34.5	32.0	35.0	24.5	35.0
96-97	31.450026054691936	34.0	32.0	35.0	24.0	35.0
98-99	31.172797379408347	34.0	31.0	35.0	21.5	35.0
100	29.872500559619578	34.0	29.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35120717979783933
1101	2	0.32399664625787494
1101	3	0.29893999018148776
1101	4	0.19028139710771796
1101	5	0.19493290181534917
1101	6	0.19272780887189356
1101	7	0.20761901492215884
1101	8	0.16552641736075913
1101	9	0.18742226124443562
1101	10-11	0.19100929051654703
1101	12-13	0.23097597073924447
1101	14-15	0.3598236427548187
1101	16-17	0.43887573296712645
1101	18-19	0.49123818663883156
1101	20-21	0.4214614735776223
1101	22-23	0.39132621728145267
1101	24-25	0.3970079298103997
1101	26-27	0.4142221199840961
1101	28-29	0.40915868226309726
1101	30-31	0.4318689834104177
1101	32-33	0.44322265434407626
1101	34-35	0.4477561029048971
1101	36-37	0.4529699575646049
1101	38-39	0.4675248179389371
1101	40-41	0.4855479826613376
1101	42-43	0.4833922796174406
1101	44-45	0.45841249725241795
1101	46-47	0.477550286866979
1101	48-49	0.48892366456383485
1101	50-51	0.48014961849158055
1101	52-53	0.47890557511638576
1101	54-55	0.4599054533718103
1101	56-57	0.4668614743424371
1101	58-59	0.4307194543229329
1101	60-61	0.45300144595386627
1101	62-63	0.44174564003382244
1101	64-65	0.44083354785433215
1101	66-67	0.4097201375302362
1101	68-69	0.4095736149897746
1101	70-71	0.3765648504794612
1101	72-73	0.37586018957357226
1101	74-75	0.36805069329515305
1101	76-77	0.3457482365612279
1101	78-79	0.3756032725085774
1101	80-81	0.3194509439799802
1101	82-83	0.27693707992872874
1101	84-85	0.2778770642381616
1101	86-87	0.3812795526118009
1101	88-89	0.30451469379649154
1101	90-91	0.31795770618325037
1101	92-93	0.3333532868371947
1101	94-95	0.2962616891981149
1101	96-97	0.25123978110274336
1101	98-99	0.2755492087550344
1101	100	0.007324081901792567
1102	1	0.2897372253226891
1102	2	0.28324569948881617
1102	3	0.27219473715477704
1102	4	0.18732035320518747
1102	5	0.15822250698333562
1102	6	0.14666710293755614
1102	7	0.1845025678393739
1102	8	-0.029573248359774595
1102	9	0.05617203820325045
1102	10-11	0.19294530628782525
1102	12-13	0.1967776995605206
1102	14-15	0.31760109133206527
1102	16-17	0.3776530207894311
1102	18-19	0.43237029263534765
1102	20-21	0.40982057059310506
1102	22-23	0.3376538931854114
1102	24-25	0.3176655551899046
1102	26-27	0.3385573537196862
1102	28-29	0.35899994392655543
1102	30-31	0.3880599829810123
1102	32-33	0.4134499379026053
1102	34-35	0.4065243589402172
1102	36-37	0.3867357815701453
1102	38-39	0.3869616600609618
1102	40-41	0.3895746166543361
1102	42-43	0.39161417065557913
1102	44-45	0.3824826062464979
1102	46-47	0.40023836002411173
1102	48-49	0.39449399151406794
1102	50-51	0.38951890647311416
1102	52-53	0.3694199342361273
1102	54-55	0.37284086348023493
1102	56-57	0.35096069391969564
1102	58-59	0.32169171014813713
1102	60-61	0.3279669055629526
1102	62-63	0.3272924188806172
1102	64-65	0.3037829394153633
1102	66-67	0.26992089434693867
1102	68-69	0.2303981913193951
1102	70-71	0.2340058611640501
1102	72-73	0.26278287872638373
1102	74-75	0.23672249740399565
1102	76-77	0.2635270894606023
1102	78-79	0.2250106384817201
1102	80-81	0.18522304475382612
1102	82-83	0.19998356580967425
1102	84-85	0.23429771858545934
1102	86-87	0.25352766805623617
1102	88-89	0.21382838134019977
1102	90-91	0.17923657384618963
1102	92-93	0.17619236194448007
1102	94-95	0.1764581460504253
1102	96-97	0.10901007148249064
1102	98-99	0.12183585813032138
1102	100	-3.038736190390523E-4
1103	1	0.21102151992435836
1103	2	0.22255667002798418
1103	3	0.20773792020965942
1103	4	0.15038939195344625
1103	5	0.12641923232635577
1103	6	0.06170272245102382
1103	7	0.16411393297953936
1103	8	0.14935423029729122
1103	9	0.16758618186671725
1103	10-11	0.19212440119923713
1103	12-13	0.21806637530276873
1103	14-15	0.2842863660334558
1103	16-17	0.33367127062991386
1103	18-19	0.3700822457631432
1103	20-21	0.3513402425488579
1103	22-23	0.29117046890440434
1103	24-25	0.28971024717520066
1103	26-27	0.2963606076412404
1103	28-29	0.2976039264492698
1103	30-31	0.2873631772429093
1103	32-33	0.2841313023453722
1103	34-35	0.277573360909642
1103	36-37	0.29083641788547965
1103	38-39	0.2994604434208554
1103	40-41	0.2961969232562751
1103	42-43	0.3100046872960647
1103	44-45	0.31262969273939945
1103	46-47	0.3006513731785887
1103	48-49	0.27889957219049677
1103	50-51	0.2625721681704647
1103	52-53	0.28338028125476455
1103	54-55	0.2334434461807362
1103	56-57	0.2277898831550189
1103	58-59	0.25559276573104484
1103	60-61	0.22133727480822074
1103	62-63	0.20219527186847586
1103	64-65	0.21448213055099785
1103	66-67	0.16884805636851752
1103	68-69	0.12396738572341093
1103	70-71	0.14679668594804696
1103	72-73	0.1234901324463209
1103	74-75	0.08828565720718728
1103	76-77	0.08098741726848147
1103	78-79	0.09564501609843035
1103	80-81	0.02772441855786667
1103	82-83	0.035544707749835425
1103	84-85	0.05147165364070361
1103	86-87	0.09707558887455292
1103	88-89	0.058249018602317904
1103	90-91	-0.006237900463503365
1103	92-93	0.013147279365178122
1103	94-95	0.0024755757044836457
1103	96-97	-0.06117808498910193
1103	98-99	-0.062385992378072075
1103	100	-0.25146474977660915
1104	1	0.17234961638740742
1104	2	0.17297237240560293
1104	3	0.1708977564158971
1104	4	0.12679368826946558
1104	5	0.09488077171896947
1104	6	0.0671898588196953
1104	7	0.04633522749772112
1104	8	-0.0190344257733841
1104	9	-0.03102430041279547
1104	10-11	0.07167013618176554
1104	12-13	0.13580477987729722
1104	14-15	0.20498796503422057
1104	16-17	0.26822084274085256
1104	18-19	0.28110873749411525
1104	20-21	0.25497270097598346
1104	22-23	0.2497326554506074
1104	24-25	0.22114023741168864
1104	26-27	0.22647793705851882
1104	28-29	0.20941856808725134
1104	30-31	0.23545601950990402
1104	32-33	0.2563032570342543
1104	34-35	0.2534344038093366
1104	36-37	0.2440559511510827
1104	38-39	0.26562694633706485
1104	40-41	0.24799483228037644
1104	42-43	0.23105603536844654
1104	44-45	0.24700824807903388
1104	46-47	0.2316432285248453
1104	48-49	0.24973705855764194
1104	50-51	0.2472532188854757
1104	52-53	0.2153159845265833
1104	54-55	0.1849977057707477
1104	56-57	0.16542767588973817
1104	58-59	0.15666026193071758
1104	60-61	0.1389089980633642
1104	62-63	0.11713683655898421
1104	64-65	0.12904440910018877
1104	66-67	0.1365907896878511
1104	68-69	0.1372647923154986
1104	70-71	0.12004370558885569
1104	72-73	0.10595960900867851
1104	74-75	0.04997755728026476
1104	76-77	0.08459339176985026
1104	78-79	0.07882356482951991
1104	80-81	0.03281583633840057
1104	82-83	0.030778158741057382
1104	84-85	0.050658307575197625
1104	86-87	0.11962453867695899
1104	88-89	0.04191449029084282
1104	90-91	0.031294153570385674
1104	92-93	0.05426011252184537
1104	94-95	-0.03061831075209298
1104	96-97	-0.16034689080627018
1104	98-99	-0.2337955014998485
1104	100	-0.38401086705256304
1105	1	0.16861754245279315
1105	2	0.16515324196748793
1105	3	0.16162650194489458
1105	4	0.12001226741377735
1105	5	0.11958881602355831
1105	6	0.08585948198435744
1105	7	0.1366639385438475
1105	8	0.02013927094257184
1105	9	0.1135117586580705
1105	10-11	0.13085288185409638
1105	12-13	0.1529639714460842
1105	14-15	0.22150275538477615
1105	16-17	0.25526705578499076
1105	18-19	0.2567565575039481
1105	20-21	0.24639910217739214
1105	22-23	0.20918499604912455
1105	24-25	0.18215518513112983
1105	26-27	0.16344977854971177
1105	28-29	0.1866898185461281
1105	30-31	0.17089701160134751
1105	32-33	0.17032813519369228
1105	34-35	0.1883361054475614
1105	36-37	0.200379348877469
1105	38-39	0.21174272642845438
1105	40-41	0.18875648976150217
1105	42-43	0.20363225572972254
1105	44-45	0.18320291086748597
1105	46-47	0.18580319251164923
1105	48-49	0.1865502141272657
1105	50-51	0.19932527863704763
1105	52-53	0.1747265951223227
1105	54-55	0.17931148579479128
1105	56-57	0.18347230591118802
1105	58-59	0.16260707603392888
1105	60-61	0.16268527990663273
1105	62-63	0.17198403048870148
1105	64-65	0.18468399240126843
1105	66-67	0.16781493277896686
1105	68-69	0.16973464764547685
1105	70-71	0.12750326466084516
1105	72-73	0.11326908234046584
1105	74-75	0.11008485147045377
1105	76-77	0.16089920571926797
1105	78-79	0.09179253641087826
1105	80-81	0.08966745068694593
1105	82-83	0.08541524275412371
1105	84-85	0.08578080543954769
1105	86-87	0.12197103097534523
1105	88-89	0.09074791028674412
1105	90-91	0.08055292612594656
1105	92-93	0.09998603652464766
1105	94-95	0.0729468778459399
1105	96-97	0.057230815292516724
1105	98-99	0.02608550568220025
1105	100	-0.007875038165948922
1106	1	0.12987591181651226
1106	2	0.131770941931876
1106	3	0.1303236795754188
1106	4	0.09643877887180707
1106	5	0.08263974926335038
1106	6	0.07840628678341233
1106	7	0.10070743418979333
1106	8	0.051474135245619834
1106	9	0.07735391312755269
1106	10-11	0.10914749933326107
1106	12-13	0.1578276459578305
1106	14-15	0.18147183289961077
1106	16-17	0.21606524231867752
1106	18-19	0.18136222436321248
1106	20-21	0.1779719340473278
1106	22-23	0.18807332732855997
1106	24-25	0.1785406949152346
1106	26-27	0.17801183774658824
1106	28-29	0.1813408177240774
1106	30-31	0.17731685158052102
1106	32-33	0.18020154682857736
1106	34-35	0.19671632631627034
1106	36-37	0.1878123139237644
1106	38-39	0.16129570729945897
1106	40-41	0.1516908768049987
1106	42-43	0.19040095786731115
1106	44-45	0.19901126508689515
1106	46-47	0.16685487058103377
1106	48-49	0.1798255747895965
1106	50-51	0.19698902584590883
1106	52-53	0.23038332775416848
1106	54-55	0.1862306308682875
1106	56-57	0.1491291975497333
1106	58-59	0.12311773082392818
1106	60-61	0.1406842842695042
1106	62-63	0.13264284664105475
1106	64-65	0.12007634047160565
1106	66-67	0.11466586668525736
1106	68-69	0.11785148353384045
1106	70-71	0.11168309700127566
1106	72-73	0.11153679293029484
1106	74-75	0.08982806823973277
1106	76-77	0.06863182938005252
1106	78-79	0.08976317170444759
1106	80-81	0.05528532890123472
1106	82-83	0.011429044040085046
1106	84-85	-0.002065785109174101
1106	86-87	0.06238348318219522
1106	88-89	0.03319268593045166
1106	90-91	0.04785213155201973
1106	92-93	0.10926639241186109
1106	94-95	0.04259437004757771
1106	96-97	0.01589967542387072
1106	98-99	0.028118065416510518
1106	100	-0.08359191588726489
1107	1	0.10368584757508614
1107	2	0.08599346237617311
1107	3	0.10014739205243472
1107	4	0.07558701429397985
1107	5	0.0753136501882068
1107	6	0.0760396658494642
1107	7	0.10166643187213253
1107	8	0.07008748290015632
1107	9	0.09063378400888666
1107	10-11	0.09626843773762772
1107	12-13	0.10826112248138031
1107	14-15	0.14909374385386087
1107	16-17	0.15076025966301643
1107	18-19	0.18216718191192172
1107	20-21	0.16938337463949438
1107	22-23	0.18425877558420467
1107	24-25	0.13109231882860684
1107	26-27	0.16003383091197065
1107	28-29	0.14002666128671137
1107	30-31	0.13242423418219573
1107	32-33	0.1268640739098572
1107	34-35	0.14253101856998285
1107	36-37	0.12064758104815354
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2216	30-31	-0.3854473489708923
2216	32-33	-0.3857594413429055
2216	34-35	-0.4220066796877475
2216	36-37	-0.44990762233472736
2216	38-39	-0.4408390974987313
2216	40-41	-0.4445290057993603
2216	42-43	-0.3959311669653047
2216	44-45	-0.41910494569416556
2216	46-47	-0.43408461545231347
2216	48-49	-0.4548535919105703
2216	50-51	-0.5102480331269277
2216	52-53	-0.45641220273392946
2216	54-55	-0.3474616648489288
2216	56-57	-0.322200542660795
2216	58-59	-0.2920916651493499
2216	60-61	-0.3257269946834427
2216	62-63	-0.3294152455750563
2216	64-65	-0.2510826642293935
2216	66-67	-0.20925043363163098
2216	68-69	-0.23238521592465844
2216	70-71	-0.20350282251793317
2216	72-73	-0.21945772235954308
2216	74-75	-0.1940090809923447
2216	76-77	-0.26043837219817334
2216	78-79	-0.18406422220753882
2216	80-81	-0.20588645140132655
2216	82-83	-0.2725016498730639
2216	84-85	-0.25618704674109694
2216	86-87	-0.26703507079631095
2216	88-89	-0.3143823061875679
2216	90-91	-0.3057570014091624
2216	92-93	-0.310272653944633
2216	94-95	-0.3661011278916604
2216	96-97	-0.28905318451374384
2216	98-99	-0.25663722058209615
2216	100	-0.0881862811673031
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	9.0
7	85.0
8	357.0
9	879.0
10	1642.0
11	2844.0
12	4485.0
13	6093.0
14	8367.0
15	11681.0
16	16590.0
17	22938.0
18	30450.0
19	39528.0
20	50032.0
21	61954.0
22	75782.0
23	92833.0
24	112963.0
25	136503.0
26	163902.0
27	195123.0
28	236514.0
29	289521.0
30	341994.0
31	426140.0
32	554014.0
33	777790.0
34	1134858.0
35	4406628.0
36	2621336.0
37	4011855.0
38	1909626.0
39	319890.0
40	638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.039426209695172	22.796574292016935	32.89184144679152	25.272158051496373
2	15.087902552839925	24.373320490944593	38.44578311585689	22.09299384035859
3	11.884221960512887	19.665712822495312	44.431613601888735	24.018451615103064
4	12.060521501237362	17.831311949776605	48.7792820529171	21.328884496068937
5	11.125523099641748	17.469263917234947	51.01972738036217	20.38548560276114
6	10.421804815761721	13.61964600159284	47.39026861961168	28.568280563033753
7	13.390838534861699	14.583276596432473	45.37852203306969	26.64736283563613
8	9.263109988107946	13.771568048522948	46.576882873559626	30.388439089809477
9	10.351517482382777	13.398228170242144	49.68259993831454	26.56765440906054
10-11	13.55454525124871	22.006422727883624	49.15202688565228	15.28700513521538
12-13	12.360215420998555	27.77891128635412	44.55859607699262	15.3022772156547
14-15	13.622604983670408	25.42175842930807	44.70981848237569	16.24581810464584
16-17	18.286649104243345	25.487989932825723	45.83709180705867	10.388269155872264
18-19	18.108951898400097	21.051753795726345	45.20631031686092	15.632983989012637
20-21	13.992633830245534	20.91795485334361	49.021384786332035	16.06802653007882
22-23	14.106953885681056	22.41915433556875	50.0562885471931	13.417603231557102
24-25	14.53750133609821	23.25976633129933	48.24292637364043	13.959805958962038
26-27	17.85612790539723	23.008214727635426	45.424348438540825	13.711308928426519
28-29	15.94595997885047	25.322756205589748	47.699202375691144	11.032081439868639
30-31	16.07570533447207	23.49595909895072	47.91328559521997	12.515049971357243
32-33	16.664381939892174	24.385637523876504	47.33786431601356	11.61211622021777
34-35	16.87518422499847	24.337762851881855	47.3163333849048	11.470719538214873
36-37	17.169961671546048	23.583530956683006	46.88195177721797	12.364555594552971
38-39	17.447583836454235	23.830636406020815	46.240861725119196	12.48091803240575
40-41	17.385332585888154	24.14786904224628	46.386780295014255	12.080018076851307
42-43	17.51854347761586	24.687516468206784	46.334982589645726	11.458957464531634
44-45	17.77153326623213	23.97239043269483	45.89750122510674	12.358575075966305
46-47	18.098177287806383	23.47955917188457	46.257621482039475	12.164642058269575
48-49	17.214002141310672	23.50930992967088	46.52345906535514	12.753228863663299
50-51	17.163327407425204	23.181378707656393	46.62085258510765	13.034441299810753
52-53	16.847521938136033	23.51069907009002	46.73655755749104	12.90522143428291
54-55	16.543699574739097	23.749187819345284	46.939379458690155	12.767733147225465
56-57	16.32786564364629	23.927105798789356	46.53482353324759	13.210205024316767
58-59	16.25306613671742	24.082799468105907	47.012853590541134	12.651280804635537
60-61	15.664441665026057	23.5466502348293	47.487088311798374	13.301819788346267
62-63	15.500001273177785	23.297041677308773	47.7658968148081	13.437060234705342
64-65	15.53053946324251	23.28848988022064	47.60711413580662	13.573856520730226
66-67	15.032723533408166	23.22460435740126	48.259901578784174	13.482770530406398
68-69	15.041651391270111	22.906543689276322	48.67436865861159	13.377436260841979
70-71	14.619765455380687	22.74301549159566	49.33074670490776	13.30647234811589
72-73	14.451753572656234	22.252991720770147	49.90337485033785	13.39187985623577
74-75	14.306570765291887	21.752536015463164	50.357645725646194	13.58324749359876
76-77	14.438771241495049	21.717519255587256	50.48624798205641	13.35746152086128
78-79	14.489744360575962	21.9656521835638	50.661736740176245	12.882866715683999
80-81	14.397139687421017	21.597786685817635	50.9489538613157	13.056119765445645
82-83	14.37884115932391	21.303769588132596	51.146784439409075	13.170604813134421
84-85	14.190275338099973	21.17429036708416	51.168806748484855	13.466627546331015
86-87	14.165337902444517	21.339474598710993	51.068236761705556	13.42695073713894
88-89	14.096725964779822	21.910019938247892	50.82247763451121	13.170776462461072
90-91	14.081262837102463	22.38267858414004	50.58553184309932	12.950526735658174
92-93	13.82761648026376	22.781794889742045	50.24424688373878	13.146341746255413
94-95	13.730188985568342	23.575082956603403	49.864567086563866	12.830160971264387
96-97	13.129521028102372	24.683912910859107	49.94373204358061	12.242834017457916
98-99	12.804258844017536	25.715467046564488	49.63694592464739	11.843328184770584
100	12.573035740460847	26.7288667979546	49.070950396808705	11.62714706477585
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	448779.0
1	1015959.0
2	1559038.5
3	1057903.0
4	346456.5
5	78425.5
6	37454.5
7	27919.0
8	25532.5
9	26943.5
10	31395.5
11	39723.5
12	53398.0
13	73276.0
14	99811.5
15	132729.5
16	170308.0
17	210207.5
18	249218.0
19	283238.5
20	310167.0
21	329637.0
22	344540.5
23	355752.5
24	362665.5
25	365421.0
26	361752.5
27	352048.0
28	336958.5
29	316694.5
30	294797.5
31	272959.0
32	250693.5
33	228115.5
34	206601.5
35	186967.0
36	168089.0
37	150114.5
38	133793.0
39	120026.0
40	108672.5
41	99847.5
42	96379.0
43	102235.0
44	121659.0
45	156300.0
46	203606.0
47	258309.0
48	320627.5
49	396019.5
50	488547.0
51	592602.5
52	685414.0
53	733244.5
54	708222.0
55	608721.0
56	462957.0
57	312737.0
58	190304.5
59	107205.5
60	57533.0
61	29599.0
62	14683.5
63	7220.0
64	3722.5
65	2096.5
66	1410.5
67	1092.0
68	869.0
69	677.5
70	528.5
71	391.5
72	299.0
73	246.0
74	195.0
75	149.5
76	113.0
77	79.5
78	44.5
79	27.0
80	26.5
81	19.5
82	13.0
83	16.0
84	10.5
85	9.5
86	11.0
87	7.5
88	6.0
89	4.5
90	3.5
91	2.0
92	1.5
93	2.0
94	1.0
95	0.0
96	0.5
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04250562553291172
2	0.0037031206513241234
3	0.0
4	0.0
5	0.0077937128207240134
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.5353074010823955E-5
14-15	1.245444165243539E-4
16-17	0.0
18-19	0.0
20-21	5.5353074010823955E-5
22-23	1.1070614802164792E-5
24-25	0.0010018906395959136
26-27	0.0013395443910619398
28-29	0.0010849202506121496
30-31	0.0010793849432110673
32-33	0.0012537471263451628
34-35	0.0016550569129236367
36-37	0.0030139748798893645
38-39	0.003799988530843065
40-41	0.004157015858212879
42-43	0.004051845017592314
44-45	0.0060417880282814355
46-47	0.006177403059607954
48-49	0.0066811160331064524
50-51	0.007658097789397494
52-53	0.007492038567365023
54-55	0.005820375732238139
56-57	0.0046524258706097535
58-59	0.005366480525349383
60-61	0.005056503310888769
62-63	0.004483598994876741
64-65	0.003476173047879745
66-67	0.0018183484812555669
68-69	0.001666127527725801
70-71	6.254897363223108E-4
72-73	8.52437339766689E-4
74-75	0.0011125967876175616
76-77	6.28257390022852E-4
78-79	6.919134251352995E-4
80-81	0.0014723917686879173
82-83	5.009453197979568E-4
84-85	0.0012094646671365035
86-87	0.0010295671766013257
88-89	3.5425967366927336E-4
90-91	2.546241404497902E-4
92-93	2.4908883304870785E-5
94-95	4.0407744027901493E-4
96-97	3.07209560760073E-4
98-99	0.0015747949556079415
100	0.003963280099174995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.8065844E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.61417012606347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.70826938019418	66.17131676232016
2	5.493014351384513	7.757692998271483
3	0.5260331041310635	1.1143617332115654
4	0.09759613585072895	0.27566680562439105
5	0.03951373017172483	0.1395114632330772
6	0.020724014143496564	0.08780454362542871
7	0.015096889028423155	0.07462380031591616
8	0.010154388376761357	0.05736349666901983
9	0.009184242375727833	0.05836838882387818
>10	0.05970635047711087	0.8517051321239376
>50	0.009783285337504987	0.48684058981000555
>100	0.0077965928912250675	1.0466452650433604
>500	9.191356154843636E-4	0.45803880744191344
>1k	9.736403415856955E-4	1.5082534375947465
>5k	3.1660504627258285E-4	1.5742430766961741
>10k+	9.181546341904903E-4	18.337563699194995
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74680	0.41337675671283336	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64339	0.35613614287824025	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59962	0.3319081023837026	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59881	0.33145974248421495	No Hit
TTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59319	0.32834889972480663	No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57159	0.3163926357384687	No Hit
TTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56997	0.31549591593949333	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54093	0.29942138324675005	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52283	0.2894024768507909	No Hit
CTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51466	0.2848801307041066	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51325	0.28409965236055396	No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50541	0.2797599713581054	No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48911	0.27073742029434106	No Hit
CTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48900	0.2706765319129292	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48203	0.2668184226543747	No Hit
TTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47392	0.2623292883520969	No Hit
CTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46392	0.2567939809510145	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46107	0.255216418341706	No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44974	0.2489449150562797	No Hit
TTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43530	0.2409519311691167	No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42685	0.23627459641520207	No Hit
ATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42597	0.23578748936390684	No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42583	0.23570999506029167	No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41142	0.22773361709533194	No Hit
ATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40479	0.22406370828841432	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40411	0.2236873073851407	No Hit
TTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38817	0.21486402738781535	No Hit
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38411	0.21261669258297594	No Hit
AATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38258	0.21176979055061032	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37953	0.21008152179328018	No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37712	0.2087475127096193	No Hit
ATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37178	0.2057916585574413	No Hit
TTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36997	0.20478976791784542	No Hit
TACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36422	0.201606966162223	No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35655	0.1973613853855928	No Hit
CCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34776	0.1924958501800414	No Hit
TATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34084	0.18866541745849238	No Hit
TATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33958	0.187967968725956	No Hit
TTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33909	0.18769673866330297	No Hit
TTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33644	0.18622988220201614	No Hit
CCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32683	0.18091045178957593	No Hit
CACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32558	0.18021853836444066	No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32402	0.1793550304098718	No Hit
TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31865	0.17638257033549054	No Hit
CTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31563	0.17471090750036367	No Hit
ACTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30864	0.17084172762700706	No Hit
TATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30209	0.1672161012792981	No Hit
ACTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29976	0.16592637465484592	No Hit
CTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29555	0.16359601023899023	No Hit
CCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29301	0.16219004215911528	No Hit
TATTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28345	0.15689828828368052	No Hit
GTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28022	0.1551103839931309	No Hit
ATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27466	0.1520327530781291	No Hit
GCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26046	0.1441726165685921	No Hit
CATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25956	0.14367443890249468	No Hit
GTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25790	0.14275557787391502	No Hit
ACTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24457	0.13537701310827216	No Hit
ATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24402	0.13507257120121263	No Hit
ATTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23974	0.13270345963354938	No Hit
GTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23962	0.13263703594473636	No Hit
CTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22887	0.1266865804885728	No Hit
TTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22642	0.12533043017530762	No Hit
TCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22611	0.12515883564587407	No Hit
AACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22320	0.12354806119215908	No Hit
CCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22078	0.12220851680109714	No Hit
TTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21644	0.11980619338902737	No Hit
GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21583	0.11946853963756135	No Hit
CTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21505	0.11903678566027694	No Hit
CATTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21314	0.11797954194667021	No Hit
CCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20759	0.11490744633906946	No Hit
ACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19867	0.10996995213730396	No Hit
CATTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19785	0.10951605693041522	No Hit
TGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19744	0.10928910932697083	No Hit
ATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19064	0.10552510029423481	No Hit
GCTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18407	0.10188840333172366	No Hit
GATTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18175	0.10060421201467254	No Hit
GTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18098	0.1001779933447892	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001760227753544202	0.0	0.0	0.0	0.0
2	0.004682870061315707	0.0	0.0	5.535307401082396E-6	0.0
3	0.008712573849303691	0.0	0.0	1.1070614802164792E-5	0.0
4	0.015604031563651274	0.0	0.0	1.1070614802164792E-5	0.0
5	0.025811138411247214	0.0	0.0	1.1070614802164792E-5	0.0
6	0.04622535210643909	5.535307401082396E-6	0.0	1.1070614802164792E-5	0.0
7	0.07084086411905251	5.535307401082396E-6	0.0	1.660592220324719E-5	0.0
8	0.16279892597323437	5.535307401082396E-6	0.0	2.2141229604329585E-5	0.0
9	0.4916792152085449	5.535307401082396E-6	0.0	2.2141229604329585E-5	0.0
10-11	1.1604716613295232	5.535307401082396E-6	0.0	2.2141229604329585E-5	0.0
12-13	1.9485859614419343	5.535307401082396E-6	0.0	3.044419070595318E-5	0.0
14-15	2.850193436852438	5.535307401082396E-6	0.0	3.321184440649438E-5	0.0
16-17	3.82256704973208	5.535307401082396E-6	0.0	4.981776660974156E-5	0.0
18-19	4.88749377001152	5.535307401082396E-6	0.0	6.365603511244755E-5	0.0
20-21	6.310601929253901	5.535307401082396E-6	0.0	7.749430361515354E-5	0.0
22-23	7.946235448507139	5.535307401082396E-6	0.0	7.749430361515354E-5	0.0
24-25	9.695381516634374	5.535307401082396E-6	0.0	7.749430361515354E-5	0.0
26-27	11.494818620154144	5.535307401082396E-6	0.0	8.026195731569474E-5	0.0
28-29	13.420834365668163	5.535307401082396E-6	0.0	8.302961101623595E-5	0.0
30-31	15.4844938326712	5.535307401082396E-6	0.0	8.579726471677715E-5	0.0
32-33	17.811354398942	5.535307401082396E-6	0.0	9.410022581840073E-5	0.0
34-35	19.96242467276923	5.535307401082396E-6	0.0	9.410022581840073E-5	0.0
36-37	21.620625640296684	5.535307401082396E-6	0.0	9.410022581840073E-5	0.0
38-39	22.837596184269053	5.535307401082396E-6	0.0	9.686787951894194E-5	0.0
40-41	23.881574533689097	5.535307401082396E-6	0.0	1.2177676282381271E-4	0.0
42-43	24.70380016566068	5.535307401082396E-6	0.0	1.328473776259775E-4	0.0
44-45	25.347885767196928	5.535307401082396E-6	0.0	1.411503387276011E-4	0.0
46-47	25.87080902503088	5.535307401082396E-6	0.0	1.466856461286835E-4	0.0
48-49	26.31883127076709	5.535307401082396E-6	0.0	1.632915683319307E-4	0.0
50-51	26.75004002027251	5.535307401082396E-6	0.0	1.7989749053517787E-4	0.0
52-53	27.209041548238766	5.535307401082396E-6	0.0	2.0757402754058985E-4	0.0
54-55	27.64615647074114	5.535307401082396E-6	0.0	2.4355352564762542E-4	0.0
56-57	28.084245607346105	5.535307401082396E-6	0.0	2.65694755251955E-4	0.0
58-59	28.502789573517852	5.535307401082396E-6	0.0	2.823006774552022E-4	0.0
60-61	28.913105305237885	5.535307401082396E-6	0.0	2.93371292257367E-4	0.0
62-63	29.322275228325893	5.535307401082396E-6	0.0	3.099772144606142E-4	0.0
64-65	29.721600607201083	5.535307401082396E-6	0.0	3.3211844406494373E-4	0.0
66-67	30.080573595122374	5.535307401082396E-6	0.0	3.570273273698145E-4	0.0
68-69	30.388339454276256	5.535307401082396E-6	0.0	3.7086559587252055E-4	0.0
70-71	30.673122717100846	5.535307401082396E-6	0.0	3.7640090327360293E-4	0.0
72-73	30.946625023442028	5.535307401082396E-6	0.0	3.930068254768501E-4	0.0
74-75	31.204163503238487	5.535307401082396E-6	0.0	4.0407744027901493E-4	0.0
76-77	31.450218987831402	5.535307401082396E-6	0.0	4.262186698833445E-4	0.0
78-79	31.676729301991095	5.535307401082396E-6	0.0	4.8157174389416846E-4	0.0
80-81	31.89578078942783	5.535307401082396E-6	0.0	5.037129734984981E-4	0.0
82-83	32.116919087754766	5.535307401082396E-6	0.0	5.258542031028276E-4	0.0
84-85	32.342933991902065	5.535307401082396E-6	5.535307401082396E-6	5.3138951050391E-4	0.0
86-87	32.57590954510623	5.535307401082396E-6	5.535307401082396E-6	5.341571642044512E-4	0.0
88	32.753781113132604	5.535307401082396E-6	5.535307401082396E-6	5.369248179049925E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTTT	4945	0.0	61.337097	1
TGCTCTA	10040	0.0	56.12583	6
TGCCCTA	11450	0.0	55.1249	6
GCTTTCT	3580	0.0	54.24963	1
GCCCTAC	26695	0.0	53.32694	7
GGCTCTA	3855	0.0	51.325718	6
GACTTTT	5005	0.0	50.924294	1
GCTTTTC	4750	0.0	50.589115	1
CCCCTAC	66855	0.0	49.13835	7
GCCCCTA	7910	0.0	47.77018	6
GGCCCTA	3795	0.0	47.678905	6
CCCCCTA	36000	0.0	46.188366	6
TTGCCCT	4970	0.0	45.394478	5
ATGCCCT	3765	0.0	45.06718	5
CACTTTT	8300	0.0	45.042118	1
CCCTTTT	10540	0.0	44.482002	1
CGCCCTA	11210	0.0	44.356506	6
GCATTTT	4340	0.0	44.207935	1
TTGCTCT	4190	0.0	43.636887	5
GTTCTAC	6600	0.0	43.22371	7
>>END_MODULE