##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248464_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15966034
Sequences flagged as poor quality	0
Sequence length	100
%GC	28
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.923405712401713	31.0	28.0	33.0	22.0	34.0
2	29.50761710766744	31.0	30.0	33.0	23.0	34.0
3	29.794445195344068	31.0	30.0	34.0	25.0	34.0
4	33.294934358776885	35.0	33.0	37.0	28.0	37.0
5	33.06412951394191	35.0	33.0	37.0	26.0	37.0
6	32.87399582137931	35.0	32.0	37.0	26.0	37.0
7	33.02552387148869	35.0	32.0	37.0	27.0	37.0
8	32.939796382746025	35.0	32.0	37.0	26.0	37.0
9	34.166157544196636	37.0	32.0	39.0	26.0	39.0
10-11	34.477001927967834	37.0	34.0	39.0	27.0	39.0
12-13	34.38712065250519	37.0	33.5	39.0	27.0	39.0
14-15	35.1009203036897	37.5	32.5	40.0	27.0	41.0
16-17	34.89060564445748	37.0	32.0	40.0	27.0	41.0
18-19	34.40674211892572	37.0	32.0	40.0	26.5	41.0
20-21	34.039726647206194	35.5	32.0	39.0	25.5	40.5
22-23	33.471693439961356	35.0	32.0	38.5	25.0	40.0
24-25	32.95543163693626	35.0	30.5	38.0	25.0	40.0
26-27	32.47861369329415	35.0	30.0	37.5	24.0	40.0
28-29	32.28434663235717	35.0	30.0	37.0	23.5	40.0
30-31	32.14950726022505	35.0	30.0	37.0	23.0	40.0
32-33	31.95081452288026	34.5	30.0	37.0	22.5	40.0
34-35	31.79680633274362	34.0	29.5	37.0	22.0	40.0
36-37	31.595177205560255	34.0	28.0	37.0	22.0	39.0
38-39	31.379187029164537	34.0	28.0	37.0	21.5	39.0
40-41	31.10916762422027	33.0	27.0	37.0	21.0	39.0
42-43	30.864551083882198	33.0	26.0	36.0	20.0	39.0
44-45	30.62148674492363	33.0	26.0	35.5	20.0	39.0
46-47	30.49619814789321	33.0	26.0	35.0	20.0	39.0
48-49	30.29055521865981	33.0	25.5	35.0	20.0	39.0
50-51	30.082248572187684	33.0	26.0	35.0	20.0	39.0
52-53	29.81381850996935	33.0	26.0	35.0	19.0	38.0
54-55	29.626406344869366	32.5	26.0	35.0	18.0	38.0
56-57	29.420140875310675	32.0	26.0	35.0	18.0	37.0
58-59	29.141706168231885	31.5	26.0	35.0	17.0	37.0
60-61	28.893765007640596	31.0	26.0	35.0	16.5	36.5
62-63	28.863612716846276	31.0	26.0	35.0	16.5	36.0
64-65	29.13264690529909	31.5	26.0	35.0	19.0	35.5
66-67	29.58767800444368	32.0	26.5	35.0	20.0	35.0
68-69	29.61873449599318	32.0	27.0	35.0	20.0	35.0
70-71	29.939467747594676	33.0	28.0	35.0	20.0	35.0
72-73	29.951608865420177	33.0	28.0	35.0	20.0	35.0
74-75	29.8641642627092	33.0	28.0	35.0	20.0	35.0
76-77	29.733406712023786	33.0	28.0	35.0	20.0	35.0
78-79	29.586473416002995	33.0	27.5	35.0	19.5	35.0
80-81	29.459351520859848	33.0	27.0	35.0	19.0	35.0
82-83	29.322178006134774	33.0	27.0	35.0	18.5	35.0
84-85	29.186797046780683	33.0	27.0	35.0	17.5	35.0
86-87	28.993244220825282	33.0	27.0	35.0	16.0	35.0
88-89	28.78881032697287	33.0	27.0	35.0	13.5	35.0
90-91	28.509692231646255	33.0	26.0	35.0	10.0	35.0
92-93	28.220249624922506	32.0	25.5	35.0	10.0	35.0
94-95	27.847673536208177	32.0	25.0	35.0	8.0	35.0
96-97	27.43456840941213	32.0	25.0	35.0	8.0	35.0
98-99	26.947364793285544	31.5	24.0	34.5	7.0	35.0
100	25.616226233766007	30.0	19.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7004575782260822
1101	2	0.6291447850018344
1101	3	0.5648703073827335
1101	4	0.5658866271901601
1101	5	0.4550878291213962
1101	6	0.26921414598773197
1101	7	0.36646237561583916
1101	8	0.5178323226480686
1101	9	0.5604447820696237
1101	10-11	0.5639057824739027
1101	12-13	0.6154994966401475
1101	14-15	0.7575618353557516
1101	16-17	0.7309972025587328
1101	18-19	0.7856024635823715
1101	20-21	0.6803589303875555
1101	22-23	0.6319453783439997
1101	24-25	0.5916128585249965
1101	26-27	0.6766425548332222
1101	28-29	0.5355481832785642
1101	30-31	0.5226939164097146
1101	32-33	0.5758184082121183
1101	34-35	0.5303787568107587
1101	36-37	0.45977750429725006
1101	38-39	0.3783481430973268
1101	40-41	0.37901832653693646
1101	42-43	0.39577754467803317
1101	44-45	0.4485487536601873
1101	46-47	0.40375845847254865
1101	48-49	0.4156254650516793
1101	50-51	0.3744650814677968
1101	52-53	0.34581849602400894
1101	54-55	0.2700501773152624
1101	56-57	0.2188516428178815
1101	58-59	0.27260004022212314
1101	60-61	0.2742202619359304
1101	62-63	0.2916245631418555
1101	64-65	0.38802636463830353
1101	66-67	0.4509238660965025
1101	68-69	0.4670290521344178
1101	70-71	0.4811886808103232
1101	72-73	0.4688455491880248
1101	74-75	0.42657061828522913
1101	76-77	0.47737855905280924
1101	78-79	0.4161327828324133
1101	80-81	0.37794782397896753
1101	82-83	0.31012393562085805
1101	84-85	0.29641317226861474
1101	86-87	0.22880184534408698
1101	88-89	0.2704644969443706
1101	90-91	0.3356691872404234
1101	92-93	0.3030444878622447
1101	94-95	0.3202416444374556
1101	96-97	0.2886048525791338
1101	98-99	0.25127008196812284
1101	100	0.3019148481840226
1102	1	0.37769331368235015
1102	2	0.4196299840721629
1102	3	0.3801265732217267
1102	4	0.37445321351553673
1102	5	0.34067039820662615
1102	6	0.23213201370960945
1102	7	0.30964907686313126
1102	8	0.35500231092899526
1102	9	0.3029194135444726
1102	10-11	0.3253996650771285
1102	12-13	0.39794334950692445
1102	14-15	0.5370163126009615
1102	16-17	0.5748466177038338
1102	18-19	0.6267835385395912
1102	20-21	0.47386668271985144
1102	22-23	0.4254115952380175
1102	24-25	0.4085247039578661
1102	26-27	0.41573558997868787
1102	28-29	0.2867035815364005
1102	30-31	0.23052720770363777
1102	32-33	0.22632606148208723
1102	34-35	0.20508938657949116
1102	36-37	0.187404204183089
1102	38-39	0.16094578779266655
1102	40-41	0.09088857716089649
1102	42-43	0.20639147220008525
1102	44-45	0.1574544130287734
1102	46-47	0.17997617866937787
1102	48-49	0.19463419411944116
1102	50-51	0.19416233260372096
1102	52-53	0.202309072132131
1102	54-55	0.20068993846713923
1102	56-57	0.1085419401824872
1102	58-59	0.1680824918620374
1102	60-61	0.15577722514915848
1102	62-63	0.16588139578771077
1102	64-65	0.20053757175661602
1102	66-67	0.18587632921675024
1102	68-69	0.24995145727450918
1102	70-71	0.2638465068675835
1102	72-73	0.17973689612509958
1102	74-75	0.2457419385549784
1102	76-77	0.24203032133466706
1102	78-79	0.21878537884895266
1102	80-81	0.21280722025463916
1102	82-83	0.13798276525660924
1102	84-85	0.061093895728784986
1102	86-87	0.12182004983826289
1102	88-89	0.10093967938696125
1102	90-91	0.14946595618879854
1102	92-93	0.08257573268100771
1102	94-95	0.15600983071872676
1102	96-97	0.07250465888064639
1102	98-99	0.09169669610983888
1102	100	0.04103096587692434
1103	1	0.3445267915735677
1103	2	0.3202355431976187
1103	3	0.2815315004738501
1103	4	0.28992312057543046
1103	5	0.2673126178143477
1103	6	0.22670608897111322
1103	7	0.2858242254971586
1103	8	0.34290595923228295
1103	9	0.3560351016191916
1103	10-11	0.24919602866045665
1103	12-13	0.3596521001173656
1103	14-15	0.44471816617531346
1103	16-17	0.41654683220830435
1103	18-19	0.5084517814845881
1103	20-21	0.3957109752430341
1103	22-23	0.2945196625227311
1103	24-25	0.2776394971426299
1103	26-27	0.3327808662548293
1103	28-29	0.20419297396895786
1103	30-31	0.19364668691467557
1103	32-33	0.08533161716653481
1103	34-35	0.17350755042864208
1103	36-37	0.08799200036379418
1103	38-39	0.08773441023204143
1103	40-41	0.11158391837635051
1103	42-43	0.0494084396723693
1103	44-45	0.04590812757777485
1103	46-47	0.13812254479200448
1103	48-49	0.02412121481702556
1103	50-51	0.0738623916102128
1103	52-53	0.04277294670565368
1103	54-55	0.02655427404981836
1103	56-57	-0.008292194668609199
1103	58-59	-0.01841697349964022
1103	60-61	-0.017894807218780784
1103	62-63	0.05415720389965628
1103	64-65	0.12133084520286985
1103	66-67	0.05327530760763466
1103	68-69	0.1074323160148758
1103	70-71	0.14130752077009845
1103	72-73	0.13940387557431677
1103	74-75	0.13202655478851
1103	76-77	0.09352085943165989
1103	78-79	0.10412266111163859
1103	80-81	0.1449879005126249
1103	82-83	0.09877459040359327
1103	84-85	7.635951363305082E-4
1103	86-87	0.06166570290646689
1103	88-89	0.11952656123700223
1103	90-91	0.07779182913479943
1103	92-93	0.05772804889352301
1103	94-95	0.12466854021530693
1103	96-97	0.09406092816310974
1103	98-99	0.11297376288410632
1103	100	0.03691652339061946
1104	1	0.2160351582265001
1104	2	0.16501314759375774
1104	3	0.07737187416821456
1104	4	0.06954015303623606
1104	5	0.1788631146401869
1104	6	0.09570666833784003
1104	7	0.13595530805892508
1104	8	0.22231912448695823
1104	9	0.07084186858229202
1104	10-11	0.10847545891866162
1104	12-13	0.17938198966802332
1104	14-15	0.285189987813105
1104	16-17	0.32691034233047134
1104	18-19	0.3534621448533244
1104	20-21	0.2732204498202293
1104	22-23	0.15800422846599105
1104	24-25	0.16153977705306488
1104	26-27	0.26261094580819844
1104	28-29	0.08016545201932956
1104	30-31	0.031091284873795644
1104	32-33	0.037699493961778074
1104	34-35	0.07272786392395147
1104	36-37	0.038118193809204115
1104	38-39	0.008169859201917262
1104	40-41	0.02793495659696177
1104	42-43	0.017032037778996312
1104	44-45	0.04485125986525773
1104	46-47	0.044594095788507815
1104	48-49	0.017135736307576366
1104	50-51	-0.003640919071720816
1104	52-53	-0.031783038572154254
1104	54-55	-0.014816634024096942
1104	56-57	-0.027148553252700935
1104	58-59	0.043445965646291995
1104	60-61	0.017322341663490448
1104	62-63	0.08483320027497498
1104	64-65	0.12866111956466852
1104	66-67	0.09441523829112697
1104	68-69	0.1148922526004803
1104	70-71	0.10462828141486469
1104	72-73	0.09117753690834718
1104	74-75	0.09828192905906263
1104	76-77	0.13165530268664227
1104	78-79	0.11090106670423339
1104	80-81	0.08206257548769003
1104	82-83	0.06118933975395535
1104	84-85	0.025047597825182066
1104	86-87	0.056671683583054744
1104	88-89	0.06158453856059154
1104	90-91	0.06955333071563174
1104	92-93	0.07130158989343371
1104	94-95	0.1254811530119646
1104	96-97	0.11206621925747129
1104	98-99	0.1047781361550939
1104	100	0.11159338238528349
1105	1	0.22594511468227907
1105	2	0.24554070434288633
1105	3	0.206882067311831
1105	4	0.21000282152967742
1105	5	0.12428154675914271
1105	6	0.11256194907993233
1105	7	0.08574616300550986
1105	8	0.20433543335951754
1105	9	0.15585564710607258
1105	10-11	0.22781468009916495
1105	12-13	0.21859593745336525
1105	14-15	0.3328416336447546
1105	16-17	0.3183384297303391
1105	18-19	0.3657808262774367
1105	20-21	0.32451679372078956
1105	22-23	0.19073296831654574
1105	24-25	0.13904542874958992
1105	26-27	0.1878457564038527
1105	28-29	0.07577845675394457
1105	30-31	0.005720212158514926
1105	32-33	0.06002150790295602
1105	34-35	0.04217184397202445
1105	36-37	-0.024230845525764266
1105	38-39	-0.06937433484553779
1105	40-41	-0.06982335045320909
1105	42-43	-0.11928246110798568
1105	44-45	-0.09438643036638084
1105	46-47	-0.06609630351244533
1105	48-49	-0.04511629841453768
1105	50-51	-0.11496085023158287
1105	52-53	-0.06153039923217207
1105	54-55	-0.03215700880011596
1105	56-57	-0.14096342296528164
1105	58-59	-0.062368872300993417
1105	60-61	-0.08195762050232602
1105	62-63	0.015676150216208384
1105	64-65	0.0677154725883895
1105	66-67	0.036440889173569246
1105	68-69	0.038505914070174896
1105	70-71	0.013090216147492839
1105	72-73	0.06208342107382947
1105	74-75	0.028262584839435334
1105	76-77	0.06710036240531991
1105	78-79	0.10865408706033008
1105	80-81	0.09251968257986576
1105	82-83	-0.03629620903902975
1105	84-85	-0.0310320213890094
1105	86-87	-0.027542037536093744
1105	88-89	0.0023748750865131285
1105	90-91	0.036545365269752494
1105	92-93	0.09233087683930563
1105	94-95	0.0512770679718777
1105	96-97	0.07319518779193857
1105	98-99	0.071632616941681
1105	100	0.07754739643023001
1106	1	0.03508040119547573
1106	2	0.02662676292681354
1106	3	0.09982164027229956
1106	4	0.09152543616505682
1106	5	0.18452427536345795
1106	6	0.07332865960285062
1106	7	0.1011236564342397
1106	8	0.22483436275094704
1106	9	0.1735820299396451
1106	10-11	0.1257216188465975
1106	12-13	0.1805214706794942
1106	14-15	0.3577304881365819
1106	16-17	0.23533745801332628
1106	18-19	0.25039774332604736
1106	20-21	0.17097038491743888
1106	22-23	0.14309497160146378
1106	24-25	0.07650511969198703
1106	26-27	0.2314525607161002
1106	28-29	0.04467648952861225
1106	30-31	0.042007249912160205
1106	32-33	0.043501817017887134
1106	34-35	0.04668810661653211
1106	36-37	0.03919941586324782
1106	38-39	-0.02422353239073871
1106	40-41	-0.05355339657393543
1106	42-43	0.007735428230930097
1106	44-45	0.01518677374865618
1106	46-47	-0.03436744982322182
1106	48-49	-0.004809963908837034
1106	50-51	0.028428392660408974
1106	52-53	0.03420823468849221
1106	54-55	-0.09571661747620297
1106	56-57	-0.03004995164240043
1106	58-59	-0.013040618267218917
1106	60-61	0.04699711009701346
1106	62-63	0.04977933280441604
1106	64-65	0.0897634163606611
1106	66-67	0.026618592997664337
1106	68-69	0.049354890900882964
1106	70-71	0.028281750457129107
1106	72-73	0.024271876846373885
1106	74-75	0.05995761475054806
1106	76-77	0.07561686009641733
1106	78-79	0.03829446577198681
1106	80-81	0.0879488862592055
1106	82-83	0.044542262355513174
1106	84-85	0.05396806460364445
1106	86-87	0.09102993975037776
1106	88-89	0.0851842265012337
1106	90-91	0.03183081789209652
1106	92-93	0.055127587333565486
1106	94-95	0.07888278877665655
1106	96-97	0.10605871458008664
1106	98-99	0.0589856769903534
1106	100	0.11073726534876016
1107	1	-0.009233969114148977
1107	2	0.06494626626283662
1107	3	0.05539605456291241
1107	4	0.11237883757372202
1107	5	0.08127890452174569
1107	6	0.03591800679877366
1107	7	0.044306440656058044
1107	8	0.1416009249275163
1107	9	0.12031530807592361
1107	10-11	0.11816208895854885
1107	12-13	0.13471755472591695
1107	14-15	0.2193355343642409
1107	16-17	0.21176910641087687
1107	18-19	0.2042068049095036
1107	20-21	0.14487124892229986
1107	22-23	0.08725647820106985
1107	24-25	0.08090155261726295
1107	26-27	0.2171913228650979
1107	28-29	-0.05677890016201559
1107	30-31	-0.003379217016174607
1107	32-33	-0.01191311931721728
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2216	24-25	0.8690718851912891
2216	26-27	0.9888195538512008
2216	28-29	1.0712094681063604
2216	30-31	1.1119892799800581
2216	32-33	1.1959091568340519
2216	34-35	1.1132035510058493
2216	36-37	1.1296151041982014
2216	38-39	1.1408685034204389
2216	40-41	1.2373699039555
2216	42-43	1.184674708015514
2216	44-45	1.210390456988204
2216	46-47	1.1189997572691084
2216	48-49	1.078462899580746
2216	50-51	1.056027757179745
2216	52-53	1.060560288975541
2216	54-55	1.0010611331639048
2216	56-57	1.0156986964179104
2216	58-59	0.9549727298245863
2216	60-61	0.9730589601874442
2216	62-63	0.820848888491323
2216	64-65	0.7166856434617479
2216	66-67	0.79313780335168
2216	68-69	0.7748647178079189
2216	70-71	0.706572209967316
2216	72-73	0.7087412353380635
2216	74-75	0.7006838052792688
2216	76-77	0.717504261207452
2216	78-79	0.6414185416698928
2216	80-81	0.6716000708263188
2216	82-83	0.6719875702012601
2216	84-85	0.6051984349990782
2216	86-87	0.5520517662124398
2216	88-89	0.4516151534282642
2216	90-91	0.44332913772628757
2216	92-93	0.388674181356258
2216	94-95	0.3248436056378239
2216	96-97	0.2935464186742216
2216	98-99	0.24457320635664814
2216	100	0.4349497076898672
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	218.0
6	2939.0
7	7535.0
8	10643.0
9	14714.0
10	22062.0
11	30934.0
12	39251.0
13	47351.0
14	57019.0
15	68833.0
16	83290.0
17	101116.0
18	122771.0
19	145763.0
20	170022.0
21	196471.0
22	221953.0
23	259658.0
24	350862.0
25	409121.0
26	471535.0
27	764035.0
28	685269.0
29	1108850.0
30	1052924.0
31	1248678.0
32	1540084.0
33	2279300.0
34	1645725.0
35	2539680.0
36	219971.0
37	42490.0
38	4720.0
39	247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.799673668413112	32.05955116159877	25.510346412422575	26.630428757565543
2	13.063535605529788	35.65742143551522	27.065229584363887	24.213813374591105
3	11.492124140768096	38.47621483747592	24.075736170428115	25.95592485132786
4	10.023845630234394	38.643243015733155	23.86898607468307	27.463925279349375
5	9.941456809129907	38.0165151049199	25.031745604250034	27.010282481700166
6	9.80621737370721	34.75177993482915	26.505261106170764	28.93674158529288
7	13.24063320922403	40.03932974212632	21.98029266378864	24.739744384861012
8	11.810966956477733	37.25069732408186	19.734362334440725	31.203973384999685
9	9.39317804283769	36.57693576250683	20.632068051464753	33.397818143190726
10-11	13.080540226834039	51.4049356277207	19.308332927262963	16.206191218182298
12-13	13.016437557808292	52.233077388849544	18.700656753848133	16.049828299494035
14-15	14.152080598099692	51.07537977183313	18.335370574808998	16.43716905525818
16-17	14.077819826764742	50.91433163677341	19.075579445715825	15.932269090746018
18-19	14.444394894812323	49.78763667921539	20.147968493615885	15.619999932356402
20-21	13.892979518763212	49.83226573321806	20.498445198768284	15.776309549250447
22-23	14.475218841952053	50.615887235347124	20.701597012382194	14.207296910318634
24-25	14.689395031439128	50.56216137637129	20.12537069762226	14.623072894567313
26-27	16.161959694718718	50.57581747008971	19.336243142222813	13.92597969296875
28-29	15.737402287881888	51.413976695778054	19.78898767220463	13.059633344135433
30-31	16.172222231269206	51.44135982674219	19.502598453692382	12.883819488296217
32-33	16.641815995130663	51.517577878138056	18.961493505525542	12.879112621205744
34-35	16.749074942468493	51.52776826104717	18.879331586040713	12.84382521044362
36-37	17.38204991922227	51.245127625307575	18.51159154490088	12.861230910569274
38-39	17.80668887464476	51.47959725001212	18.500599460078814	12.213114415264306
40-41	18.006007380546208	51.69383090733844	18.52668481645434	11.773476895661018
42-43	18.25442206981456	51.70871747065151	18.7984491693596	11.238411290174334
44-45	18.333729643536586	51.42805238008938	18.870757370876547	11.36746060549748
46-47	18.22006597150129	51.05539370104631	19.2940429293902	11.430497398062194
48-49	18.029122393892607	51.215078238456215	19.70550661953869	11.050292748112485
50-51	18.319476360732015	51.11078493247179	19.963773710927942	10.60596499586826
52-53	18.553032644174504	51.11629162257828	20.065646860078086	10.265028873169129
54-55	18.515872275461813	51.317930820817935	19.98465304293055	10.181543860789702
56-57	18.207641754455906	51.427854728468084	19.684805852675048	10.67969766440097
58-59	17.79154923896389	51.10065404802114	19.901870405538208	11.205926307476759
60-61	17.748881782413843	50.47878828267558	20.439484220063665	11.332845714846904
62-63	17.762186692279375	49.66894561939543	20.853148708046284	11.715718980278913
64-65	17.437385516592133	49.49638183029334	20.969124761849613	12.097107891264912
66-67	16.684558848628694	49.29882271739236	21.5648341585763	12.451784275402645
68-69	16.323742668550757	49.30239471095317	21.24461910473753	13.12924351575854
70-71	15.785406956515748	49.48064608968053	21.367330174725563	13.366616779078152
72-73	15.801327510534119	50.077943877527055	21.25161476201735	12.869113849921474
74-75	15.469314470034035	50.84886443995897	20.98907304782944	12.692748042177545
76-77	14.534146972079334	51.94125157962566	20.996766966006355	12.52783448228865
78-79	13.772919177288593	53.057728074392486	20.609740206237483	12.559612542081444
80-81	13.03853768185402	54.65355445392497	20.053260000045096	12.254647864175913
82-83	12.347438018475234	56.04435817396253	19.904613647441288	11.703590160120948
84-85	11.645127487116875	57.53443284736286	19.73517311322133	11.085266552298934
86-87	11.132982328377276	58.744767644275	19.356793507373926	10.765456519973796
88-89	10.825236886750464	59.723649766504906	19.069281631322653	10.381831715421976
90-91	10.858737153952205	60.545563432566006	18.502214080163743	10.09348533331805
92-93	11.004124600361225	61.114348613537494	17.855554180382555	10.02597260571872
94-95	11.34689161621501	61.076982911319874	17.427568816126833	10.148556656338275
96-97	11.74839299489331	60.68266619164906	17.225742471810236	10.343198341647394
98-99	12.246657340022328	59.98292091660337	17.104888671534425	10.66553307183988
100	13.135439820335879	58.83344214931524	17.04638892953134	10.984729100817548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1536695.0
1	1599391.0
2	1070727.5
3	298647.5
4	116154.5
5	112180.0
6	97450.0
7	70376.0
8	45916.0
9	33054.0
10	31061.0
11	35352.0
12	44180.0
13	57838.5
14	76201.5
15	98017.5
16	122845.5
17	149550.0
18	176844.0
19	203586.5
20	227605.0
21	248496.5
22	266656.5
23	280664.0
24	289767.0
25	294430.5
26	292211.0
27	283093.0
28	269114.5
29	251930.5
30	232843.5
31	212645.0
32	193144.0
33	174927.0
34	159847.5
35	147978.5
36	138633.0
37	133193.0
38	131589.0
39	133855.0
40	141241.0
41	154919.5
42	177863.5
43	214145.5
44	264800.0
45	327654.5
46	395288.0
47	455568.0
48	496255.5
49	508349.0
50	492752.5
51	461805.0
52	424543.0
53	383707.0
54	340104.0
55	290740.0
56	235623.0
57	179204.5
58	127439.0
59	85733.0
60	55899.5
61	36749.0
62	25258.0
63	18261.5
64	13984.0
65	11082.5
66	9075.0
67	7674.0
68	6543.0
69	5620.5
70	4777.0
71	4075.0
72	3507.5
73	3008.5
74	2636.5
75	2310.0
76	2040.5
77	1876.0
78	1725.0
79	1570.5
80	1481.0
81	1409.5
82	1362.0
83	1252.0
84	1135.0
85	1089.5
86	1067.0
87	1044.5
88	1001.0
89	949.0
90	912.5
91	888.0
92	851.5
93	810.5
94	761.0
95	696.0
96	680.5
97	658.5
98	619.0
99	606.5
100	652.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.07114252669135E-4
2	4.572206222284131E-4
3	1.8789888584729307E-4
4	3.757977716945862E-5
5	2.7558503257602983E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.07114252669135E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.757977716945862E-5
22-23	1.5658240487274424E-5
24-25	1.4092416438546982E-4
26-27	2.3174195921166142E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	4.3843073364368386E-5
42-43	5.950131385164281E-5
44-45	3.444812907200373E-5
46-47	3.069015135505787E-4
48-49	2.818483287709396E-5
50-51	2.818483287709396E-5
52-53	0.0
54-55	1.5658240487274424E-5
56-57	9.394944292364654E-6
58-59	2.818483287709396E-5
60-61	0.0
62-63	9.394944292364654E-6
64-65	1.221342758007405E-4
66-67	1.8163558965238332E-4
68-69	3.5700788310985684E-4
70-71	8.705981710924579E-4
72-73	3.695344754996764E-4
74-75	9.113095963593714E-4
76-77	0.0014155049400496078
78-79	7.484638952917174E-4
80-81	0.0012902390161514123
82-83	0.0014280315324394274
84-85	0.0017756444712569196
86-87	0.0022078119087056937
88-89	0.0024301589236249903
90-91	0.0023456044249937085
92-93	0.0022923664073369755
94-95	0.0010804185936219351
96-97	0.0019416218204220286
98-99	0.0019165686356423892
100	0.0015157176791681642
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.5966034E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.41891174296359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.4057290229555	68.13773340863558
2	3.9091116032718016	5.583689931749274
3	0.3714955806644264	0.7959543026512105
4	0.0914352341335256	0.26120819667117906
5	0.04669965158058745	0.16676191473305624
6	0.031553974096462684	0.13521302946810165
7	0.021314884065979117	0.10656000767437643
8	0.017339445377419552	0.09906914552654919
9	0.012751913917029126	0.0819655033134755
>10	0.07968932142198101	1.051219681094494
>50	0.005900560160805805	0.29532884987870434
>100	0.0046696865404363425	0.6750320936499016
>500	6.834625447475029E-4	0.35403503211540793
>1k	8.481810126384097E-4	1.311109223267642
>5k	2.3854446506375155E-4	1.302882386550406
>10k+	5.389337914403276E-4	19.6422372930207
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	609284	3.8161261588194035	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	191860	1.2016760079553883	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	157107	0.9840076752936892	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	148563	0.9304940726043801	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	106588	0.6675922148230424	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	92894	0.581822636729948	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89333	0.5595190389798744	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	87865	0.5503245201657468	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76338	0.47812750492702194	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	74165	0.464517362295483	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	74110	0.464172881004763	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69936	0.43802988268720966	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68116	0.4266306836124738	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61650	0.3861322104161873	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59608	0.3733425595861815	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55780	0.3493666617520669	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52786	0.3306143529445071	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50715	0.31764306652484897	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50024	0.3133151288541663	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46033	0.2883183137402814	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44083	0.27610488616020734	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43716	0.2738062564566755	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43667	0.2734993549431249	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35411	0.2217895815579498	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30152	0.18885090686891937	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29465	0.18454802238301635	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29461	0.1845229691982367	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28929	0.1811908956225447	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26565	0.16638446341777802	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26457	0.16570802742872776	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25458	0.1594509945300129	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24460	0.15320022492749294	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24048	0.15061974689519014	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23745	0.14872196814813246	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23716	0.1485403325584801	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23301	0.14594106463759254	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23199	0.14530220842571173	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21502	0.13467339478294987	No Hit
TAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21459	0.13440407304656873	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21239	0.13302614788368858	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20900	0.13090289047361417	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20454	0.12810946037068444	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19496	0.12210922261596087	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18798	0.11773744187191384	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18470	0.11568308071998343	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18291	0.1145619507010946	No Hit
GAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17113	0.10718378778349087	No Hit
TAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16968	0.10627560983522896	No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16576	0.10382039772682432	No Hit
TATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16248	0.10176603657489393	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0028623263610737643	0.0	0.0	6.2632961949097695E-6	0.0
2	0.007252896993705513	0.0	0.0	2.5053184779639078E-5	0.0
3	0.012620541832743184	0.0	0.0	4.3843073364368386E-5	0.0
4	0.021025885326312097	0.0	0.0	8.1422850533827E-5	0.0
5	0.03380927286012293	0.0	0.0	1.0021273911855631E-4	0.0
6	0.05752211225405132	0.0	0.0	1.0647603531346607E-4	0.0
7	0.08665896615277156	0.0	0.0	1.0647603531346607E-4	0.0
8	0.1719525337350528	0.0	0.0	1.0647603531346607E-4	0.0
9	0.46947789288185154	0.0	0.0	1.0647603531346607E-4	0.0
10-11	1.1331023095654187	0.0	0.0	1.0647603531346607E-4	0.0
12-13	2.003875852951334	0.0	0.0	1.0960768341092096E-4	0.0
14-15	3.0433356211066567	0.0	0.0	1.2839757199565026E-4	0.0
16-17	4.187536491529455	0.0	3.1316480974548847E-6	1.4718746058037957E-4	0.0
18-19	5.425740042893558	0.0	6.2632961949097695E-6	1.5971405297019912E-4	0.0
20-21	7.05925153359939	0.0	6.2632961949097695E-6	1.7224064536001867E-4	0.0
22-23	8.920368076380147	0.0	6.2632961949097695E-6	1.7537229345747355E-4	0.0
24-25	10.82373994693986	0.0	6.2632961949097695E-6	1.9103053394474797E-4	0.0
26-27	12.71026041908717	0.0	6.2632961949097695E-6	1.9416218204220285E-4	0.0
28-29	14.711872090464043	6.2632961949097695E-6	6.2632961949097695E-6	2.0042547823711262E-4	0.0
30-31	16.889297617680135	6.2632961949097695E-6	6.2632961949097695E-6	2.0042547823711262E-4	0.0
32-33	19.263985658554905	6.2632961949097695E-6	6.2632961949097695E-6	2.0042547823711262E-4	0.0
34-35	21.34588339220623	6.2632961949097695E-6	6.2632961949097695E-6	2.0042547823711262E-4	0.0
36-37	22.900051446714944	6.2632961949097695E-6	6.2632961949097695E-6	2.0668877443202237E-4	0.0
38-39	24.022540600878088	6.2632961949097695E-6	6.2632961949097695E-6	2.411369035040261E-4	0.0
40-41	24.935112251420733	6.2632961949097695E-6	6.2632961949097695E-6	2.661900882836652E-4	0.0
42-43	25.63753152473557	6.2632961949097695E-6	6.2632961949097695E-6	2.881116249658494E-4	0.0
44-45	26.179353620316732	6.2632961949097695E-6	6.2632961949097695E-6	3.25691402135308E-4	0.0
46-47	26.62016440651448	6.2632961949097695E-6	6.2632961949097695E-6	3.4448129072003734E-4	0.0
48-49	27.014097552341426	6.2632961949097695E-6	6.2632961949097695E-6	3.9458766027931544E-4	0.0
50-51	27.409010904022878	6.2632961949097695E-6	6.2632961949097695E-6	4.634839184233229E-4	0.0
52-53	27.838400569609206	6.2632961949097695E-6	6.2632961949097695E-6	5.229852322749657E-4	0.0
54-55	28.291863214120674	6.2632961949097695E-6	6.2632961949097695E-6	6.075397309062477E-4	0.0
56-57	28.775085910502256	6.2632961949097695E-6	6.2632961949097695E-6	6.576461004655257E-4	0.0
58-59	29.277239419632956	6.2632961949097695E-6	6.2632961949097695E-6	6.858309333426197E-4	0.0
60-61	29.784494383514403	6.2632961949097695E-6	6.2632961949097695E-6	7.265423586095333E-4	0.0
62-63	30.257955732776217	6.2632961949097695E-6	6.2632961949097695E-6	7.860436724611761E-4	0.0
64-65	30.694877012036926	6.2632961949097695E-6	6.2632961949097695E-6	8.424133382153639E-4	0.0
66-67	31.071329298183883	6.2632961949097695E-6	6.2632961949097695E-6	8.956513558720969E-4	0.0
68-69	31.389407663794277	6.2632961949097695E-6	6.2632961949097695E-6	9.207045406517361E-4	0.0
70-71	31.664757822762997	9.394944292364654E-6	6.2632961949097695E-6	9.457577254313751E-4	0.0
72-73	31.918483951618793	1.2526592389819539E-5	6.2632961949097695E-6	9.551526697237398E-4	0.0
74-75	32.15452253202017	1.878988858472931E-5	6.2632961949097695E-6	9.864691506982887E-4	0.0
76-77	32.381372856903596	1.878988858472931E-5	6.2632961949097695E-6	0.0010303122240626571	0.0
78-79	32.600068370141265	1.878988858472931E-5	6.2632961949097695E-6	0.0011179983707913937	0.0
80-81	32.813289762504574	1.878988858472931E-5	6.2632961949097695E-6	0.0012338693503972245	0.0
82-83	33.0231759496441	1.878988858472931E-5	6.2632961949097695E-6	0.0012933706642488671	0.0
84-85	33.23799135088902	1.878988858472931E-5	6.2632961949097695E-6	0.0013246871452234161	3.1316480974548847E-6
86-87	33.45710963661983	1.878988858472931E-5	6.2632961949097695E-6	0.0013403453857106905	6.2632961949097695E-6
88	33.62446804259593	1.878988858472931E-5	6.2632961949097695E-6	0.0013466086819056004	6.2632961949097695E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTGCT	1205	0.0	44.85432	7
TTGCTGA	5650	0.0	44.088047	9
TACCCGA	9120	0.0	37.414097	6
GCCTGCT	1380	0.0	37.122814	7
GAAAACA	6460	0.0	36.887085	1
CTTGCTG	3835	0.0	36.6437	8
GACACGC	770	0.0	36.62301	2
ACCCGAC	9650	0.0	35.359226	7
TGAAAAA	14945	0.0	35.065323	1
CTGCTGA	5680	0.0	34.83592	9
CCTGCTG	4755	0.0	34.199444	8
TACTGGC	385	0.0	34.181473	2
ACTGGCC	605	0.0	34.181473	3
TGACGCC	815	0.0	34.024193	3
ACTTGCC	880	0.0	33.647385	3
TCGCGAC	615	0.0	33.625675	6
CTACCCG	9595	0.0	33.455627	5
CCCGACG	11055	0.0	32.438385	8
ATTGCTG	1135	0.0	31.471045	8
TCGACAC	1230	0.0	30.950907	5
>>END_MODULE