##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248463_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19978754
Sequences flagged as poor quality	0
Sequence length	100
%GC	27
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.93056779216562	31.0	28.0	33.0	22.0	34.0
2	29.50082337467091	31.0	30.0	33.0	23.0	34.0
3	29.824524942846786	31.0	30.0	34.0	25.0	34.0
4	33.22704874388062	35.0	33.0	37.0	27.0	37.0
5	33.00206424284517	35.0	33.0	37.0	26.0	37.0
6	32.78034666225932	35.0	32.0	37.0	25.0	37.0
7	32.98774988670465	35.0	32.0	37.0	27.0	37.0
8	32.90627673777854	35.0	32.0	37.0	26.0	37.0
9	34.135431969381074	37.0	32.0	39.0	26.0	39.0
10-11	34.45030753669623	37.0	34.0	39.0	27.0	39.0
12-13	34.389298526825044	37.0	33.5	39.0	27.0	39.0
14-15	35.08192753161684	37.0	32.5	40.0	27.0	41.0
16-17	34.87068562934405	37.0	32.0	40.0	27.0	41.0
18-19	34.39085718258506	37.0	32.0	40.0	26.5	41.0
20-21	34.04040059254947	35.5	32.0	39.0	26.5	40.5
22-23	33.50290456051464	35.0	32.0	38.5	25.0	40.0
24-25	33.0150637271974	35.0	31.0	38.0	25.0	40.0
26-27	32.53713880254995	35.0	30.0	38.0	24.0	40.0
28-29	32.35823275065101	35.0	30.0	37.0	24.0	40.0
30-31	32.22391268744788	35.0	30.0	37.0	23.5	40.0
32-33	32.02376792366531	34.5	30.0	37.0	23.0	40.0
34-35	31.87442004641531	34.0	30.0	37.0	22.0	40.0
36-37	31.664776316881422	34.0	28.0	37.0	22.0	40.0
38-39	31.469564142989096	34.0	28.0	37.0	22.0	39.5
40-41	31.222113225879852	33.5	27.0	37.0	21.0	39.0
42-43	30.991213866490373	33.0	26.0	37.0	20.5	39.0
44-45	30.74819493247677	33.0	26.0	36.0	20.0	39.0
46-47	30.60067334529471	33.0	26.0	35.5	20.0	39.0
48-49	30.41181221811931	33.0	26.0	35.0	20.0	39.0
50-51	30.208948340822456	33.0	26.0	35.0	20.0	39.0
52-53	29.943563797822428	33.0	26.0	35.0	19.5	38.5
54-55	29.756473601907306	33.0	26.0	35.0	18.0	38.0
56-57	29.54916580383341	32.0	26.0	35.0	18.0	38.0
58-59	29.257810121692273	32.0	26.0	35.0	17.0	37.0
60-61	28.996112870702547	31.0	26.0	35.0	15.5	37.0
62-63	28.93713264100454	31.0	26.0	35.0	16.5	36.0
64-65	29.177152689301845	31.0	26.0	35.0	18.5	36.0
66-67	29.610482941028256	32.0	26.5	35.0	20.0	36.0
68-69	29.60995638166424	32.0	27.0	35.0	20.0	35.5
70-71	29.923823402600583	32.5	28.0	35.0	20.0	35.0
72-73	29.933593706594515	33.0	28.0	35.0	20.0	35.0
74-75	29.855783448757613	33.0	28.0	35.0	20.0	35.0
76-77	29.762685826153124	33.0	28.0	35.0	20.0	35.0
78-79	29.671922308067863	33.0	28.0	35.0	20.0	35.0
80-81	29.623094838647095	33.0	28.0	35.0	20.0	35.0
82-83	29.575467869517787	33.0	28.0	35.0	19.5	35.0
84-85	29.532161740416846	33.0	28.0	35.0	19.0	35.0
86-87	29.435627291872155	33.0	28.0	35.0	19.0	35.0
88-89	29.344814045961023	33.0	28.0	35.0	18.0	35.0
90-91	29.198964960477515	33.0	27.0	35.0	17.0	35.0
92-93	29.04477654111963	33.0	27.0	35.0	15.5	35.0
94-95	28.817290732945608	33.0	27.0	35.0	14.0	35.0
96-97	28.55938065506988	33.0	26.0	35.0	10.0	35.0
98-99	28.243151274599008	32.0	25.5	35.0	10.0	35.0
100	26.906241149973617	31.0	24.0	34.0	8.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6909757405860049
1101	2	0.6101977108472489
1101	3	0.5713746705006777
1101	4	0.5449113393871627
1101	5	0.39582762518536896
1101	6	0.27795565597880056
1101	7	0.3804654707001305
1101	8	0.44238389526442035
1101	9	0.43408191577858446
1101	10-11	0.47798419184614716
1101	12-13	0.5907191897788948
1101	14-15	0.7229556623668927
1101	16-17	0.7115058649240709
1101	18-19	0.7491423975637588
1101	20-21	0.5930226861920858
1101	22-23	0.5259607212759008
1101	24-25	0.5004582744338961
1101	26-27	0.6216754686831649
1101	28-29	0.4344371588656415
1101	30-31	0.3939500746441098
1101	32-33	0.3888201560678297
1101	34-35	0.3243215664919603
1101	36-37	0.3221553605077858
1101	38-39	0.30243493974218083
1101	40-41	0.35264170007334883
1101	42-43	0.27527111971149054
1101	44-45	0.2614996756807031
1101	46-47	0.3198346086150785
1101	48-49	0.30785090023677597
1101	50-51	0.3245973180490509
1101	52-53	0.27541074101375784
1101	54-55	0.17587778564251266
1101	56-57	0.17859893778399183
1101	58-59	0.19650376073764164
1101	60-61	0.19446858067009032
1101	62-63	0.21151289625047198
1101	64-65	0.2906353169065419
1101	66-67	0.32456910080502865
1101	68-69	0.32305939757255686
1101	70-71	0.362934906729361
1101	72-73	0.34490546937903943
1101	74-75	0.32951010765999555
1101	76-77	0.34435529342591664
1101	78-79	0.34302164779273525
1101	80-81	0.2645248336385144
1101	82-83	0.25205180555407125
1101	84-85	0.21900147819697935
1101	86-87	0.19616679806215842
1101	88-89	0.24285753489431627
1101	90-91	0.30999954830838305
1101	92-93	0.2505096846651327
1101	94-95	0.3051607599422006
1101	96-97	0.2838971984394867
1101	98-99	0.2585372141939857
1101	100	0.16793343542245154
1102	1	0.49466594457435775
1102	2	0.414749434645735
1102	3	0.4037719852427877
1102	4	0.3950670368177427
1102	5	0.310647962964282
1102	6	0.24052933991966796
1102	7	0.3158454349454871
1102	8	0.3528339505255218
1102	9	0.299113294008734
1102	10-11	0.28565020586678713
1102	12-13	0.35255745426114515
1102	14-15	0.48617013403241316
1102	16-17	0.5159105573970422
1102	18-19	0.5900798689996236
1102	20-21	0.43802058880712735
1102	22-23	0.3579087161958867
1102	24-25	0.3272084307957286
1102	26-27	0.436965943620379
1102	28-29	0.24014895904854683
1102	30-31	0.23037931280607893
1102	32-33	0.25029206959465
1102	34-35	0.2420033572786906
1102	36-37	0.145148311176321
1102	38-39	0.188951293157551
1102	40-41	0.17861640159991055
1102	42-43	0.16821095779820894
1102	44-45	0.1830289082716341
1102	46-47	0.17072770126886638
1102	48-49	0.15603648499000045
1102	50-51	0.1135744911427814
1102	52-53	0.11010181526575735
1102	54-55	0.09932126571382938
1102	56-57	0.04915116349918591
1102	58-59	0.062327556515921145
1102	60-61	0.0642306504392458
1102	62-63	0.07561838685355937
1102	64-65	0.14445677712750182
1102	66-67	0.14966920301563746
1102	68-69	0.204274241956238
1102	70-71	0.22853194203951688
1102	72-73	0.1819027412552856
1102	74-75	0.17777975513123323
1102	76-77	0.20002516020144867
1102	78-79	0.1822186212114012
1102	80-81	0.1930663622128428
1102	82-83	0.16296948469493344
1102	84-85	0.07118183864936256
1102	86-87	0.07834020129944719
1102	88-89	0.08738455159624436
1102	90-91	0.13360553815455845
1102	92-93	0.1431284226617393
1102	94-95	0.08969204371002348
1102	96-97	0.028856778611402234
1102	98-99	0.11918097351320256
1102	100	0.1232903108298089
1103	1	0.2693746260271759
1103	2	0.3195601776597137
1103	3	0.26864920162749684
1103	4	0.23462737476031492
1103	5	0.27514637359492156
1103	6	0.13844198843882793
1103	7	0.18290184963115763
1103	8	0.24361998234050475
1103	9	0.2342187300237768
1103	10-11	0.17609461109388036
1103	12-13	0.22113085832405233
1103	14-15	0.3388784940509808
1103	16-17	0.34672301273437967
1103	18-19	0.41260513408411725
1103	20-21	0.31798831015697004
1103	22-23	0.2423664968375263
1103	24-25	0.20169060986604848
1103	26-27	0.28649040804235426
1103	28-29	0.11434970605794348
1103	30-31	0.02642152594958702
1103	32-33	0.062427267007834075
1103	34-35	0.08073404278317042
1103	36-37	0.011088073013716837
1103	38-39	-0.012151694916884281
1103	40-41	0.030332754734324396
1103	42-43	-0.008593836496512353
1103	44-45	0.04146790483956053
1103	46-47	0.03659068269341148
1103	48-49	8.349021883695684E-4
1103	50-51	-0.0742301156003542
1103	52-53	-0.07584600244168271
1103	54-55	-0.07979727776611512
1103	56-57	-0.052362971918011425
1103	58-59	-0.07796724392834875
1103	60-61	-0.05811849272228997
1103	62-63	-0.007614665719451352
1103	64-65	0.020507877114145145
1103	66-67	0.04365573408863099
1103	68-69	0.10569050399040236
1103	70-71	0.11979242841698223
1103	72-73	0.07491798315312082
1103	74-75	0.07267988259362923
1103	76-77	0.020856722126442406
1103	78-79	0.030233211734824295
1103	80-81	0.01936352358979221
1103	82-83	1.9855138851454512E-4
1103	84-85	-0.045514313451445076
1103	86-87	-0.03060657960743285
1103	88-89	-0.015876156014147824
1103	90-91	0.028995684959891577
1103	92-93	0.01517204791965554
1103	94-95	0.004937174715941239
1103	96-97	-0.030392524034816404
1103	98-99	-0.024837768136247718
1103	100	-0.0751781363673345
1104	1	0.217741867615878
1104	2	0.23269858489729955
1104	3	0.2284521430735751
1104	4	0.1891985487862513
1104	5	0.17437484866807296
1104	6	0.10973772479706412
1104	7	0.17816650049480387
1104	8	0.3152033991712315
1104	9	0.1417348628028563
1104	10-11	0.17625695840798983
1104	12-13	0.24918115728461032
1104	14-15	0.32140697629190385
1104	16-17	0.3588231510917268
1104	18-19	0.4211079242025022
1104	20-21	0.3000301489703787
1104	22-23	0.19759378802436345
1104	24-25	0.2187739904381658
1104	26-27	0.25348096452053426
1104	28-29	0.07470617021205328
1104	30-31	0.10725224226904118
1104	32-33	0.09646791133118171
1104	34-35	0.09166217593610781
1104	36-37	0.010421260914547759
1104	38-39	0.05308911319280085
1104	40-41	0.05387961894194504
1104	42-43	0.049340721594681725
1104	44-45	0.025649427147357073
1104	46-47	0.030536459403826655
1104	48-49	0.07719533294155667
1104	50-51	0.021436865574038677
1104	52-53	0.023843626601841805
1104	54-55	-0.025610339854395647
1104	56-57	-0.023890362812998944
1104	58-59	-0.007536826643409711
1104	60-61	0.007747447084671677
1104	62-63	0.0446627959637631
1104	64-65	0.11380979405484126
1104	66-67	0.1382000584351779
1104	68-69	0.13238754096706273
1104	70-71	0.10311972860250407
1104	72-73	0.09305563716023002
1104	74-75	0.12065816085793202
1104	76-77	0.13942008077470192
1104	78-79	0.12168463451315858
1104	80-81	0.11401743826050748
1104	82-83	0.02891736019630642
1104	84-85	0.02255197154979882
1104	86-87	0.07809618212070646
1104	88-89	0.06656999131753949
1104	90-91	0.07862856112169325
1104	92-93	0.04600585978724325
1104	94-95	0.07074287319992578
1104	96-97	0.05149382804064473
1104	98-99	0.060314259168318785
1104	100	0.11440594119344993
1105	1	0.1573814787627157
1105	2	0.17573998495431198
1105	3	0.17393930094213061
1105	4	0.20743041121901484
1105	5	0.19018609467619285
1105	6	0.1289030709512744
1105	7	0.08775263213161821
1105	8	0.22050731125186473
1105	9	0.20159329938127257
1105	10-11	0.16477083865181186
1105	12-13	0.204195145065313
1105	14-15	0.28085019490251995
1105	16-17	0.2115623319382891
1105	18-19	0.3018454854515653
1105	20-21	0.18327793510900392
1105	22-23	0.12903984108769606
1105	24-25	0.08595278638050274
1105	26-27	0.17151128858776588
1105	28-29	0.02807115392590731
1105	30-31	-0.03964474393466588
1105	32-33	-0.0048002057610148086
1105	34-35	0.003359591635870629
1105	36-37	-0.0928416598496895
1105	38-39	-0.11898101644414893
1105	40-41	-0.08700653344430975
1105	42-43	-0.16159473738782637
1105	44-45	-0.19794485865529055
1105	46-47	-0.11731221895853139
1105	48-49	-0.12413392158991599
1105	50-51	-0.12716102947593555
1105	52-53	-0.15148838331722203
1105	54-55	-0.20061149660524435
1105	56-57	-0.1849084987498415
1105	58-59	-0.16947079852079838
1105	60-61	-0.12461869295308503
1105	62-63	-0.16645519951351062
1105	64-65	-0.02336314794029093
1105	66-67	-0.02988380860716333
1105	68-69	-0.07223093048760632
1105	70-71	-0.08775761794223058
1105	72-73	-0.11820085121302881
1105	74-75	-0.08354997675469278
1105	76-77	-0.08109256404890175
1105	78-79	-0.08331726407889306
1105	80-81	-0.11102808505111383
1105	82-83	-0.09176051501168914
1105	84-85	-0.1212674439795407
1105	86-87	-0.10089031618939615
1105	88-89	-0.12051674428700565
1105	90-91	-0.07556967440146778
1105	92-93	-0.07945123728628545
1105	94-95	-0.054626385523505405
1105	96-97	0.010184195591723011
1105	98-99	0.013111597281163512
1105	100	0.019414307660330365
1106	1	0.025121977176940646
1106	2	0.11063724938495412
1106	3	0.14607604958172615
1106	4	0.13287265112332136
1106	5	0.12529250813368975
1106	6	0.043811165622436476
1106	7	0.04360463053058794
1106	8	0.1784397462395475
1106	9	0.09237716468160784
1106	10-11	0.059919351809071486
1106	12-13	0.14393322661541674
1106	14-15	0.2702547238957109
1106	16-17	0.23192761903930403
1106	18-19	0.21142110328182184
1106	20-21	0.1404527581671502
1106	22-23	0.08021760336244199
1106	24-25	0.09271594939101391
1106	26-27	0.17053648785359599
1106	28-29	0.021234779420900907
1106	30-31	-0.013114487268239827
1106	32-33	-0.013535227192868149
1106	34-35	-0.0368836442397793
1106	36-37	-0.08098248896236981
1106	38-39	-0.04813856719761489
1106	40-41	-0.068573873014671
1106	42-43	-0.045746028374711756
1106	44-45	-0.10648161149000046
1106	46-47	-0.052017800846257956
1106	48-49	-0.06758268123101985
1106	50-51	-0.10238436164258857
1106	52-53	-0.09130794922706187
1106	54-55	-0.11612562446157071
1106	56-57	-0.11091961352404667
1106	58-59	-0.05780675905900523
1106	60-61	-0.06752660504139385
1106	62-63	-0.03732936628064465
1106	64-65	-0.00294572230626855
1106	66-67	0.005905116697149992
1106	68-69	0.020022716242767302
1106	70-71	0.02242775006435238
1106	72-73	0.0032287583244539064
1106	74-75	0.014700364892959783
1106	76-77	0.013016367803754036
1106	78-79	0.0243930885431638
1106	80-81	0.06488699189280567
1106	82-83	0.01617949021591869
1106	84-85	0.042522248370364224
1106	86-87	0.03313087662775516
1106	88-89	0.0973956345571203
1106	90-91	0.07712096033215587
1106	92-93	0.08201014562001774
1106	94-95	0.08219683162364788
1106	96-97	0.08452623150693483
1106	98-99	0.06618513741969423
1106	100	0.0010736158874209423
1107	1	0.030769451010431936
1107	2	0.07574792749736048
1107	3	0.10343166275694671
1107	4	0.08161376968779166
1107	5	0.08450955501294999
1107	6	0.004143607089197587
1107	7	0.02644378840168571
1107	8	0.12244094583640219
1107	9	0.1964121690652476
1107	10-11	0.15706907486202226
1107	12-13	0.1338317066029191
1107	14-15	0.18751311806254733
1107	16-17	0.20771637626940986
1107	18-19	0.20778343234914587
1107	20-21	0.1286652115631668
1107	22-23	0.08417370354766263
1107	24-25	0.04757346818867347
1107	26-27	0.15252713601772427
1107	28-29	-0.03419113773339433
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2216	9	0.3609606065695701
2216	10-11	0.49072079428468385
2216	12-13	0.49751594471946703
2216	14-15	0.49869390726283314
2216	16-17	0.48178103962039387
2216	18-19	0.6565212384021777
2216	20-21	0.6188583410060104
2216	22-23	0.8103872720036946
2216	24-25	0.9213580832101798
2216	26-27	1.0137356230892038
2216	28-29	1.0997776849542689
2216	30-31	1.1412758108556034
2216	32-33	1.267117346403289
2216	34-35	1.2108569139932968
2216	36-37	1.2114954991008489
2216	38-39	1.2363590884892197
2216	40-41	1.2894190354199466
2216	42-43	1.2833681676719983
2216	44-45	1.2932449846951712
2216	46-47	1.1934563061675263
2216	48-49	1.1906430758354354
2216	50-51	1.2281889471683023
2216	52-53	1.1695705378572185
2216	54-55	1.212444500708866
2216	56-57	1.157761115504961
2216	58-59	1.1567206186398593
2216	60-61	1.134659338870371
2216	62-63	0.9886011520533344
2216	64-65	0.8713221617973375
2216	66-67	0.915932324730008
2216	68-69	0.9395956815767441
2216	70-71	0.8562602323764423
2216	72-73	0.8254046228077456
2216	74-75	0.8409021195012336
2216	76-77	0.8221483799850908
2216	78-79	0.7848892834045671
2216	80-81	0.742810770469827
2216	82-83	0.687541780974307
2216	84-85	0.6345679440194019
2216	86-87	0.5568771188701582
2216	88-89	0.4734244578651108
2216	90-91	0.4694104299160742
2216	92-93	0.4222503320539488
2216	94-95	0.3667114840592305
2216	96-97	0.38318979984934387
2216	98-99	0.37161378197968276
2216	100	0.5731059584325386
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	378.0
6	4343.0
7	10495.0
8	14665.0
9	19975.0
10	28286.0
11	38286.0
12	46065.0
13	54883.0
14	66726.0
15	81339.0
16	99498.0
17	121564.0
18	147571.0
19	175879.0
20	206057.0
21	236676.0
22	269143.0
23	316845.0
24	433985.0
25	487899.0
26	550692.0
27	902761.0
28	763328.0
29	1280918.0
30	1224028.0
31	1512161.0
32	1958357.0
33	2962897.0
34	2249583.0
35	3287777.0
36	347482.0
37	69971.0
38	7859.0
39	380.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.500673793702052	31.97540156207619	25.826978346453245	26.696946297768516
2	12.848408245483704	35.854812182967756	27.516749633583775	23.780029937964763
3	11.09192957742108	38.74332001281765	25.04244268873069	25.12230772103058
4	9.68850904085591	39.00266533191516	24.707131327034975	26.601694300193955
5	9.543396580377076	38.210866035587706	26.006324537529828	26.239412846505385
6	9.52961330821732	35.131294974651574	27.341955359178055	27.99713635795305
7	12.4565275692368	40.876543151790145	22.741748559494752	23.925180719478302
8	11.438841481305591	37.68267530597754	20.390130435561698	30.48835277715517
9	9.120964200269947	36.90738671690937	21.37152296884981	32.60012611397087
10-11	11.843754120001677	52.8812382393817	20.09250676994171	15.182500870674916
12-13	11.453319724873664	53.52940016414441	20.025162736665845	14.99211737431608
14-15	12.239386900704618	52.54817692835098	19.713621780417338	15.498814390527057
16-17	12.214495458525592	52.2944523967811	20.20778673184524	15.283265412848069
18-19	12.413792171423703	51.28000224638634	21.13666598027084	15.16953960191912
20-21	11.862694706260136	51.178865103115314	21.580527889909423	15.377912300715124
22-23	12.155103886246344	51.65985472115212	21.940062170551546	14.244979222049986
24-25	12.240812492044665	51.60161323559539	21.554176789306588	14.603397483053355
26-27	13.291476450673361	51.69364133481368	20.92288374824535	14.091998466267611
28-29	12.971937589301113	52.34283379233759	21.236872429581947	13.44835618877934
30-31	13.18512155462748	52.2820241943016	21.12546658315128	13.407387667919632
32-33	13.46964129995294	52.37163939252668	20.7395491230334	13.419170184486982
34-35	13.575413661933073	52.33259291345196	20.66776286449095	13.42423056012402
36-37	13.966987130428654	52.1815950083774	20.40246353701537	13.448954324178574
38-39	14.259285138602737	52.287915452585274	20.396604813293163	13.05619459551882
40-41	14.433406964086256	52.50358950233335	20.34534752671771	12.717656006862693
42-43	14.629644849510544	52.51929025361056	20.51916732294756	12.331897573931332
44-45	14.695873335005777	52.30742687179397	20.55783976052954	12.438860032670716
46-47	14.694131750314085	52.05364463153258	20.80670664257434	12.445516975578991
48-49	14.626497115835285	52.11372604529573	21.09936268278673	12.160414156082252
50-51	14.783067748636313	52.1174048485218	21.249048176139556	11.850479226702333
52-53	14.919599463500063	52.09430358105174	21.378388296346916	11.60770865910128
54-55	14.901835327489573	52.23288863835604	21.320632639163296	11.544643394991093
56-57	14.777057014798947	52.30826610962151	21.075611256288962	11.839065619290574
58-59	14.608910928752895	52.14553234266732	21.199168736699615	12.046387991880172
60-61	14.604038870492122	51.7858996612101	21.520653890628015	12.089407577669759
62-63	14.665971494765326	51.2786622327226	21.74715972617208	12.308206546339996
64-65	14.52481205393514	51.23668227876467	21.740936376508685	12.497569290791505
66-67	14.146181561599768	51.142726911456585	22.015100213985143	12.695991312958506
68-69	13.959566995926437	51.082844289670746	21.88107201250572	13.076516701897098
70-71	13.700444578120788	51.222153740625565	21.92317395958131	13.154227721672337
72-73	13.759956922098343	51.504047637960625	21.870236330321774	12.865759109619255
74-75	13.609655497175277	51.8479859537621	21.752470695126203	12.789887853936424
76-77	13.146932872524019	52.41910249048808	21.765809585001577	12.668155051986329
78-79	12.739850047069107	53.05260966076081	21.538763784904376	12.668776507265711
80-81	12.31556336793746	53.94954246410975	21.22992508662544	12.504969081327355
82-83	11.924433399909873	54.732783112093095	21.177673969563994	12.165109518433031
84-85	11.535326042071166	55.60001349455117	21.049342506024246	11.815317957353415
86-87	11.265427533632327	56.325716127424705	20.825321524734584	11.58353481420838
88-89	11.105800624222608	56.96524970268351	20.628592853257032	11.300356819836853
90-91	11.117732740022925	57.47133389727854	20.296236416511913	11.114696946186612
92-93	11.217219627134224	57.832679508639906	19.910969088425855	11.039131775800014
94-95	11.448265478519074	57.83964407227642	19.627580088955902	11.084510360248608
96-97	11.703525366570446	57.65017707507519	19.483846809381365	11.162450748972997
98-99	12.002721438813738	57.2874332669992	19.407154801291043	11.302690492896016
100	12.56295402000253	56.46774753021627	19.448005778220253	11.521292671560936
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1914052.0
1	1951080.5
2	1279602.5
3	362202.0
4	154593.5
5	154189.0
6	136178.5
7	99803.0
8	66413.0
9	48613.0
10	45892.0
11	52527.5
12	65243.0
13	84128.0
14	110297.5
15	143486.5
16	181580.5
17	222888.5
18	264518.5
19	305185.0
20	343412.5
21	376354.5
22	403256.0
23	425657.5
24	442167.0
25	451103.0
26	451418.5
27	443002.5
28	426889.0
29	405555.0
30	381064.0
31	353495.5
32	326497.0
33	301649.5
34	277593.5
35	255438.0
36	237048.5
37	221075.5
38	207887.0
39	197102.5
40	189600.5
41	188381.5
42	194617.5
43	209664.5
44	236369.5
45	273708.5
46	317701.0
47	361619.5
48	396414.5
49	417831.5
50	427322.0
51	430647.5
52	430373.5
53	423048.5
54	402880.5
55	362332.5
56	301728.0
57	232529.0
58	166071.0
59	111372.5
60	71950.5
61	46072.0
62	30640.0
63	21819.0
64	16550.0
65	13053.0
66	10646.5
67	8956.0
68	7634.5
69	6412.5
70	5381.5
71	4580.0
72	3960.0
73	3496.5
74	3122.0
75	2766.5
76	2453.5
77	2195.5
78	1995.0
79	1844.5
80	1720.5
81	1615.0
82	1525.5
83	1452.5
84	1405.5
85	1392.0
86	1329.0
87	1295.0
88	1250.0
89	1212.5
90	1223.0
91	1153.0
92	1099.0
93	1091.0
94	1056.0
95	1019.5
96	991.0
97	948.5
98	898.0
99	872.0
100	890.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.354625919113875E-4
2	5.405742520279293E-4
3	2.4025522312352412E-4
4	2.0021268593627012E-5
5	3.0031902890440514E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.5026585742033765E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.5026585742033765E-5
22-23	1.2513292871016882E-5
24-25	1.1512229441335531E-4
26-27	2.0271534451047347E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	4.755051290986415E-5
42-43	6.006380578088103E-5
44-45	3.0031902890440514E-5
46-47	2.7028712601396464E-4
48-49	3.0031902890440514E-5
50-51	2.5026585742033765E-5
52-53	2.5026585742033765E-6
54-55	1.7518610019423634E-5
56-57	1.2513292871016882E-5
58-59	3.0031902890440514E-5
60-61	0.0
62-63	1.2513292871016882E-5
64-65	8.50903915229148E-5
66-67	1.5266217302640594E-4
68-69	2.52768515994541E-4
70-71	7.533002308352162E-4
72-73	3.253456146464389E-4
74-75	8.30882646635521E-4
76-77	0.001403991460128094
78-79	7.58305547983623E-4
80-81	0.0010611272354622316
82-83	0.0012263027013596545
84-85	0.001601701487490161
86-87	0.0021572916909633104
88-89	0.0023249698154349367
90-91	0.0022724139853766657
92-93	0.002062190665143582
94-95	0.0010185820397007742
96-97	0.0018194327834458546
98-99	0.0017593689776649734
100	0.0014215100701475177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9978754E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.00729201920193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.39766024240414	67.029222267618
2	4.861534136781421	6.904087482235141
3	0.42010580248126583	0.8949172618724521
4	0.09433557199620483	0.26794054034131853
5	0.04649124448888358	0.16506086868791342
6	0.029244128283017302	0.1245927814103527
7	0.020054635135851227	0.09968177334009547
8	0.01578841692713535	0.08968741850128084
9	0.012833678894781712	0.08201563064661903
>10	0.08768644145426525	1.1664245957555612
>50	0.00709190658552139	0.3485309564619477
>100	0.005071391505101865	0.7046998461572365
>500	6.357337837227385E-4	0.316885097919826
>1k	8.188411362079462E-4	1.2701999618667668
>5k	1.3526082095691218E-4	0.686226689551873
>10k+	5.125673215209301E-4	19.849826827633617
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	789096	3.949675740539175	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	243720	1.219895895409694	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	198802	0.9950670597375592	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	186098	0.9314795106841998	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	129668	0.6490294639996068	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114481	0.5730137124667535	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107271	0.5369253758267407	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	103966	0.5203828026512565	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	94531	0.4731576353560387	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91771	0.4593429600264361	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86904	0.43498208146514045	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81816	0.40951502781404686	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	81704	0.40895443229342526	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	75397	0.3773858970384239	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70938	0.3550671878736782	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67561	0.3381642318635086	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61258	0.30661571787710085	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59001	0.2953187170731468	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58529	0.29295620737909883	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55169	0.27613834176045216	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	54638	0.27348051835464815	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51593	0.25823932763774954	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48422	0.24236746696015177	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43593	0.21819679045049556	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36588	0.18313454382590627	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36420	0.18229365054497393	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35369	0.17703306222199844	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32416	0.16225236068275328	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32084	0.16059059538948225	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31348	0.15690668196825489	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28776	0.1440330062625527	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28666	0.14348242137622796	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28421	0.14225611867486831	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28083	0.14056432147870684	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27415	0.13722076962357113	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27242	0.13635484975689677	No Hit
TAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25988	0.13007818205279467	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25887	0.1295726450208056	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25060	0.12543324773907322	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24850	0.12438213113790779	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24397	0.12211472246967954	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24304	0.1216492279748777	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23824	0.11924667574364246	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22546	0.11284988042797864	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21803	0.10913092978671243	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20248	0.10134766162093992	No Hit
TATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20139	0.1008020820517636	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001681786561864669	0.0	0.0	1.5015951445220257E-5	0.0
2	0.003979227132983368	0.0	0.0	3.0031902890440514E-5	0.0
3	0.006792215370387963	0.0	0.0	6.506912292928779E-5	0.0
4	0.011797532518794715	0.0	0.0	8.008507437450803E-5	0.0
5	0.019055242383984507	0.0	0.0	9.009570867132155E-5	0.0
6	0.033055114448078196	0.0	0.0	1.0010634296813505E-4	0.0
7	0.049918027921060544	0.0	0.0	1.1011697726494856E-4	5.005317148406752E-6
8	0.10134265630379152	5.005317148406752E-6	0.0	1.2012761156176206E-4	5.005317148406752E-6
9	0.2831307698167764	5.005317148406752E-6	0.0	1.2012761156176206E-4	5.005317148406752E-6
10-11	0.6865843585641026	5.005317148406752E-6	0.0	1.2012761156176206E-4	5.005317148406752E-6
12-13	1.2223109609337999	5.005317148406752E-6	0.0	1.4014888015538907E-4	5.005317148406752E-6
14-15	1.8455555336433893	5.005317148406752E-6	0.0	1.4515419730379582E-4	5.005317148406752E-6
16-17	2.5279028912413657	5.005317148406752E-6	0.0	1.5516483160060932E-4	5.005317148406752E-6
18-19	3.2718857242048225	5.005317148406752E-6	0.0	1.6517546589742284E-4	5.005317148406752E-6
20-21	4.260042943619006	5.005317148406752E-6	0.0	1.701807830458296E-4	5.005317148406752E-6
22-23	5.394102655250673	5.005317148406752E-6	2.502658574203376E-6	1.701807830458296E-4	5.005317148406752E-6
24-25	6.552583309249416	7.507975722610128E-6	7.507975722610128E-6	1.7518610019423634E-4	5.005317148406752E-6
26-27	7.724448181302998	1.0010634296813504E-5	1.0010634296813504E-5	1.801914173426431E-4	5.005317148406752E-6
28-29	8.975101750589651	1.0010634296813504E-5	1.0010634296813504E-5	1.8519673449104984E-4	5.005317148406752E-6
30-31	10.358456288114864	1.0010634296813504E-5	1.0010634296813504E-5	1.9520736878786334E-4	5.005317148406752E-6
32-33	11.882332601923023	1.0010634296813504E-5	1.0010634296813504E-5	1.9771002736206672E-4	5.005317148406752E-6
34-35	13.234176165340442	1.0010634296813504E-5	1.0010634296813504E-5	2.002126859362701E-4	5.005317148406752E-6
36-37	14.284617048690823	1.0010634296813504E-5	1.0010634296813504E-5	2.0772066165888024E-4	5.005317148406752E-6
38-39	15.066780440862328	1.0010634296813504E-5	1.0010634296813504E-5	2.32747247400914E-4	5.005317148406752E-6
40-41	15.71928359496293	1.0010634296813504E-5	1.0010634296813504E-5	2.5026585742033764E-4	5.005317148406752E-6
42-43	16.248741037604248	1.0010634296813504E-5	1.0010634296813504E-5	2.7779510173657474E-4	5.005317148406752E-6
44-45	16.679363487833125	1.0010634296813504E-5	1.0010634296813504E-5	2.9781637033020174E-4	5.005317148406752E-6
46-47	17.051576389598672	1.0010634296813504E-5	1.0010634296813504E-5	3.253456146464389E-4	5.005317148406752E-6
48-49	17.39616995133931	1.0010634296813504E-5	1.0010634296813504E-5	3.7790144470470984E-4	5.005317148406752E-6
50-51	17.747891084699276	1.0010634296813504E-5	1.0010634296813504E-5	4.3295993333718414E-4	5.005317148406752E-6
52-53	18.121755741123796	1.0010634296813504E-5	1.0010634296813504E-5	4.85515763395455E-4	5.005317148406752E-6
54-55	18.510378575160395	1.0010634296813504E-5	1.0010634296813504E-5	5.706061549183698E-4	5.005317148406752E-6
56-57	18.91803663031238	1.0010634296813504E-5	1.0010634296813504E-5	6.106486921056238E-4	5.005317148406752E-6
58-59	19.343105681165103	1.0010634296813504E-5	1.0010634296813504E-5	6.306699606992508E-4	5.005317148406752E-6
60-61	19.77253936857123	1.251329287101688E-5	1.0010634296813504E-5	6.456859121444711E-4	5.005317148406752E-6
62-63	20.18611621125121	1.5015951445220257E-5	1.0010634296813504E-5	6.832257907575217E-4	5.005317148406752E-6
64-65	20.579273862624262	1.5015951445220257E-5	1.0010634296813504E-5	7.18263010796369E-4	5.005317148406752E-6
66-67	20.942104297395122	1.5015951445220257E-5	1.0010634296813504E-5	8.133640366160973E-4	5.005317148406752E-6
68-69	21.267855342730584	2.002126859362701E-5	1.0010634296813504E-5	8.50903915229148E-4	5.005317148406752E-6
70-71	21.56708070983806	2.002126859362701E-5	1.0010634296813504E-5	8.684225252485716E-4	5.005317148406752E-6
72-73	21.851950827363908	2.002126859362701E-5	1.0010634296813504E-5	8.90946452416402E-4	5.005317148406752E-6
74-75	22.12776132085114	2.002126859362701E-5	1.0010634296813504E-5	9.18475696732639E-4	5.005317148406752E-6
76-77	22.399830339769935	2.002126859362701E-5	1.0010634296813504E-5	9.635235510682999E-4	5.005317148406752E-6
78-79	22.667029185103335	2.002126859362701E-5	1.0010634296813504E-5	0.0010511166011654182	5.005317148406752E-6
80-81	22.930418984086796	2.2523927167830387E-5	1.0010634296813504E-5	0.0011887628227466038	5.005317148406752E-6
82-83	23.19399648246332	2.502658574203376E-5	1.0010634296813504E-5	0.0012137894084886375	5.005317148406752E-6
84-85	23.462579297988253	2.502658574203376E-5	1.0010634296813504E-5	0.0012313080185080612	5.005317148406752E-6
86-87	23.73274129107351	2.502658574203376E-5	1.0010634296813504E-5	0.0012413186528048745	5.005317148406752E-6
88	23.936702959553934	2.502658574203376E-5	1.0010634296813504E-5	0.0012413186528048745	5.005317148406752E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTGCT	1255	0.0	40.445694	7
TTGCTGA	5250	0.0	40.195675	9
CTTGCTG	3520	0.0	34.982506	8
GCCTGCT	1175	0.0	33.199577	7
ACTTGCC	1095	0.0	32.620667	3
CTGCTGA	5050	0.0	32.387703	9
TGAAAAA	20060	0.0	32.356503	1
CCTGCTG	4040	0.0	32.1085	8
TGACGCC	810	0.0	31.33301	3
TGCCAAA	2510	0.0	31.083265	6
ACTGCCA	1210	0.0	29.520355	4
GCTTGCC	490	0.0	28.775215	3
CATTGCC	835	0.0	28.706291	3
ACTGGCC	675	0.0	28.547853	3
ATGCGCC	530	0.0	28.377066	3
ATTGCCA	700	0.0	28.19971	4
CTGCCAA	2035	0.0	28.176615	5
TCGCGAC	690	0.0	27.927177	6
TTGCCAA	1445	0.0	27.321518	5
TGCTTGC	685	0.0	26.75878	6
>>END_MODULE