##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248462_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25434842
Sequences flagged as poor quality	0
Sequence length	100
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.82556545859416	31.0	28.0	33.0	18.0	34.0
2	29.374991360276585	31.0	29.0	33.0	23.0	34.0
3	29.712316671752866	31.0	30.0	34.0	25.0	34.0
4	33.15540678412707	35.0	33.0	37.0	27.0	37.0
5	32.90065812085642	35.0	32.0	37.0	26.0	37.0
6	32.69776690572719	35.0	32.0	37.0	25.0	37.0
7	32.89718084350593	35.0	32.0	37.0	26.0	37.0
8	32.76397518018787	35.0	32.0	37.0	25.0	37.0
9	33.991213941883345	37.0	32.0	39.0	25.0	39.0
10-11	34.310468785298525	37.0	33.5	39.0	27.0	39.0
12-13	34.1465643269968	37.0	32.5	39.0	27.0	39.0
14-15	34.88302699108569	38.0	32.0	40.0	27.0	41.0
16-17	34.67440259703599	37.5	32.0	40.0	26.0	41.0
18-19	34.178742745875915	36.5	32.0	39.5	25.0	41.0
20-21	33.813249262566686	35.5	32.0	39.0	25.0	40.5
22-23	33.217454387961205	35.0	31.5	38.5	24.5	40.0
24-25	32.68050697936319	35.0	30.5	38.0	23.5	40.0
26-27	32.20571570682452	35.0	30.0	37.0	22.0	40.0
28-29	32.00330228117792	35.0	30.0	37.0	22.0	40.0
30-31	31.875549079487108	35.0	30.0	37.0	22.0	40.0
32-33	31.66220130244961	34.5	29.5	37.0	21.5	40.0
34-35	31.477184407121538	34.0	28.0	37.0	21.0	39.5
36-37	31.301726564686348	34.0	27.5	37.0	20.0	39.0
38-39	31.081937898415095	34.0	26.5	37.0	20.0	39.0
40-41	30.801549524074105	33.0	26.0	36.5	19.0	39.0
42-43	30.52388971002847	33.0	26.0	35.5	18.0	39.0
44-45	30.30170741772251	33.0	25.0	35.0	18.0	39.0
46-47	30.162976204058985	33.0	25.0	35.0	18.0	39.0
48-49	29.936772695501705	33.0	25.5	35.0	18.0	39.0
50-51	29.70043906700895	33.0	26.0	35.0	17.5	38.5
52-53	29.39018052480924	32.5	26.0	35.0	16.5	38.0
54-55	29.153488962109535	32.0	25.5	35.0	15.0	37.0
56-57	28.90533399027995	31.0	26.0	35.0	15.0	37.0
58-59	28.601358424007508	31.0	25.5	35.0	14.0	36.0
60-61	28.334949515314463	31.0	25.0	35.0	13.0	35.5
62-63	28.311503075191112	31.0	25.5	35.0	14.0	35.0
64-65	28.603264313574268	31.0	26.0	35.0	16.5	35.0
66-67	29.1064807479441	32.0	26.0	35.0	18.5	35.0
68-69	29.19743629624277	32.0	26.0	35.0	19.0	35.0
70-71	29.54778024569604	32.5	27.0	35.0	20.0	35.0
72-73	29.598280441451138	33.0	27.0	35.0	20.0	35.0
74-75	29.539635237364557	33.0	27.0	35.0	19.5	35.0
76-77	29.42385077524759	33.0	27.0	35.0	19.0	35.0
78-79	29.291232966967122	33.0	27.0	35.0	18.0	35.0
80-81	29.162512823944414	33.0	27.0	35.0	17.5	35.0
82-83	29.02242182200306	33.0	27.0	35.0	17.0	35.0
84-85	28.877199905546888	33.0	27.0	35.0	15.0	35.0
86-87	28.665769046255527	32.5	26.0	35.0	13.5	35.0
88-89	28.452399409440012	32.0	25.5	35.0	10.0	35.0
90-91	28.159837576345076	32.0	25.0	35.0	10.0	35.0
92-93	27.848572383504486	32.0	25.0	35.0	9.0	35.0
94-95	27.434818584680023	32.0	24.5	35.0	8.0	35.0
96-97	26.947054438160066	31.5	23.5	35.0	7.0	35.0
98-99	26.34443056497068	31.0	21.5	35.0	7.0	35.0
100	25.002721188517704	29.0	17.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6919302976587218
1101	2	0.6028677451229996
1101	3	0.5529267621111025
1101	4	0.5244868862201812
1101	5	0.4171297563011791
1101	6	0.3123608711379404
1101	7	0.3603457887706796
1101	8	0.46590822636051854
1101	9	0.4784141904376469
1101	10-11	0.52468590437649
1101	12-13	0.6089441184551845
1101	14-15	0.738431803710391
1101	16-17	0.7608893465398339
1101	18-19	0.7840139786151141
1101	20-21	0.7319941901183924
1101	22-23	0.6469253915222026
1101	24-25	0.592763494479307
1101	26-27	0.6863251592590061
1101	28-29	0.5497400327345261
1101	30-31	0.5038598877109699
1101	32-33	0.4948829875506817
1101	34-35	0.48382614099172017
1101	36-37	0.4474909872482442
1101	38-39	0.37716147201206596
1101	40-41	0.4285934461499856
1101	42-43	0.37512500707723007
1101	44-45	0.4101490492963116
1101	46-47	0.45494324380602436
1101	48-49	0.4220767976671773
1101	50-51	0.3866079511784193
1101	52-53	0.3670553477664207
1101	54-55	0.33105932266463967
1101	56-57	0.22886049967114275
1101	58-59	0.3089100398618463
1101	60-61	0.31870233820554716
1101	62-63	0.340846268991676
1101	64-65	0.38306517077253943
1101	66-67	0.4454720939623087
1101	68-69	0.4563300006425095
1101	70-71	0.4756958275315881
1101	72-73	0.5023483593064739
1101	74-75	0.4858092236382916
1101	76-77	0.5046253754175538
1101	78-79	0.46613310008263653
1101	80-81	0.4443673501246117
1101	82-83	0.3796724799542126
1101	84-85	0.2853059713054762
1101	86-87	0.260594939595169
1101	88-89	0.27437243045566717
1101	90-91	0.29793588726582243
1101	92-93	0.2617310259652825
1101	94-95	0.29058235865320725
1101	96-97	0.30938398380544996
1101	98-99	0.2845207625953705
1101	100	0.19644789823404807
1102	1	0.5364418373464837
1102	2	0.4934563416154454
1102	3	0.5034477219710283
1102	4	0.4642871830125159
1102	5	0.4299378912061158
1102	6	0.382642837741173
1102	7	0.4250833515105157
1102	8	0.4461607312110445
1102	9	0.45979081824923185
1102	10-11	0.4336467286207295
1102	12-13	0.47889307285679905
1102	14-15	0.6748724565795001
1102	16-17	0.713479893884319
1102	18-19	0.769045133944303
1102	20-21	0.6209014492221812
1102	22-23	0.5271088345446842
1102	24-25	0.5217775252515011
1102	26-27	0.5807493016082788
1102	28-29	0.4625084657644578
1102	30-31	0.41484505642311476
1102	32-33	0.380105618822526
1102	34-35	0.4060415264400099
1102	36-37	0.3975542765717215
1102	38-39	0.3847268887174877
1102	40-41	0.377004179630557
1102	42-43	0.4268849500138785
1102	44-45	0.3671886243844469
1102	46-47	0.37571014706439954
1102	48-49	0.35419899244975994
1102	50-51	0.2586820664350924
1102	52-53	0.3479462869934018
1102	54-55	0.2449676344696563
1102	56-57	0.2618832167323575
1102	58-59	0.3120284879534978
1102	60-61	0.3177713685682306
1102	62-63	0.36052133598280633
1102	64-65	0.36697047948700856
1102	66-67	0.38638581318120657
1102	68-69	0.4060048085071841
1102	70-71	0.45521289910647766
1102	72-73	0.427571809603009
1102	74-75	0.43548346466383947
1102	76-77	0.4088243229939579
1102	78-79	0.3896905754876059
1102	80-81	0.37452643063288704
1102	82-83	0.30009735942570614
1102	84-85	0.2420485805330621
1102	86-87	0.2691802965441745
1102	88-89	0.2150556312411922
1102	90-91	0.23049878211554287
1102	92-93	0.17645998200494262
1102	94-95	0.2215180781710302
1102	96-97	0.17543863773975232
1102	98-99	0.20733935741737852
1102	100	0.1923917825488921
1103	1	0.2967759903483831
1103	2	0.2539201256301702
1103	3	0.23547477225325153
1103	4	0.271281113239624
1103	5	0.22127191712549887
1103	6	0.18473643402042228
1103	7	0.2487819698594791
1103	8	0.2763662640799538
1103	9	0.2877123265293804
1103	10-11	0.2746410598251572
1103	12-13	0.327690057781993
1103	14-15	0.4492047199995781
1103	16-17	0.4188034642223357
1103	18-19	0.5111640890069609
1103	20-21	0.38697511594961753
1103	22-23	0.30327477483742626
1103	24-25	0.2680166008038114
1103	26-27	0.36758428121451203
1103	28-29	0.20922889076766893
1103	30-31	0.16347051754410913
1103	32-33	0.13165040015581297
1103	34-35	0.10685047212342269
1103	36-37	0.0795221279034628
1103	38-39	0.09925965073804832
1103	40-41	0.1511941267572503
1103	42-43	0.12890149447980548
1103	44-45	0.1007480250681958
1103	46-47	0.09032461602413022
1103	48-49	0.11226874790254371
1103	50-51	0.08255473745842323
1103	52-53	0.1353020008542316
1103	54-55	0.09101084265775938
1103	56-57	0.044953477541522346
1103	58-59	0.02730837344792647
1103	60-61	0.003681552672148314
1103	62-63	0.08022882209172977
1103	64-65	0.20632456085459694
1103	66-67	0.17324713897875554
1103	68-69	0.18962057021031242
1103	70-71	0.20720374933259578
1103	72-73	0.20427551549581935
1103	74-75	0.20534446509540416
1103	76-77	0.1960066261083213
1103	78-79	0.21312439168492503
1103	80-81	0.1796280843644631
1103	82-83	0.1570195373991261
1103	84-85	0.09041954376087702
1103	86-87	0.12758557480701782
1103	88-89	0.16532033023330328
1103	90-91	0.16141724587824058
1103	92-93	0.1765097341322246
1103	94-95	0.18251214642307545
1103	96-97	0.1425779887355212
1103	98-99	0.18130850055697678
1103	100	0.21306199804805814
1104	1	0.2634790886081362
1104	2	0.22973327666333176
1104	3	0.22073844415585597
1104	4	0.23646979864752637
1104	5	0.19379579228999688
1104	6	0.10813763350774508
1104	7	0.12509369258373226
1104	8	0.29440045291943306
1104	9	0.15183998698309153
1104	10-11	0.17393358618155474
1104	12-13	0.2964516587164212
1104	14-15	0.4176256552170585
1104	16-17	0.4409932749578829
1104	18-19	0.48053768956836507
1104	20-21	0.3540698073487647
1104	22-23	0.27649099653370257
1104	24-25	0.2879001869066471
1104	26-27	0.38415624845175245
1104	28-29	0.19165560044617536
1104	30-31	0.15658749982348397
1104	32-33	0.1855826064012973
1104	34-35	0.16314821570430738
1104	36-37	0.11336438250479475
1104	38-39	0.08049345549208908
1104	40-41	0.10312759207594979
1104	42-43	0.10157702180401884
1104	44-45	0.06463743106514386
1104	46-47	0.11677033160574268
1104	48-49	0.14264968914842768
1104	50-51	0.12292335373083318
1104	52-53	0.09248443978137644
1104	54-55	0.04811227081582814
1104	56-57	0.08210229727759355
1104	58-59	0.0620259991595411
1104	60-61	0.07516726269159335
1104	62-63	0.15086475152872936
1104	64-65	0.18174529394729433
1104	66-67	0.18762750684155804
1104	68-69	0.1926454750519575
1104	70-71	0.19732952172792295
1104	72-73	0.22591225826555927
1104	74-75	0.1960681042639223
1104	76-77	0.21538444151753922
1104	78-79	0.21639213712968797
1104	80-81	0.21930615478268223
1104	82-83	0.19491451887927624
1104	84-85	0.142352337957373
1104	86-87	0.19400952961999707
1104	88-89	0.19230391708740768
1104	90-91	0.20741837456425571
1104	92-93	0.15852538757520307
1104	94-95	0.20153832361838298
1104	96-97	0.28530882128639234
1104	98-99	0.28202819711330207
1104	100	0.2484420829879248
1105	1	0.17707783838154256
1105	2	0.20232622551876744
1105	3	0.19912977286284317
1105	4	0.16548748304457916
1105	5	0.09920355580200635
1105	6	0.10917895118326726
1105	7	0.11029752137653759
1105	8	0.2117903140312123
1105	9	0.20451144122868214
1105	10-11	0.1681958203136844
1105	12-13	0.2434060071743005
1105	14-15	0.3300687372024953
1105	16-17	0.3369994351933485
1105	18-19	0.37975733024503455
1105	20-21	0.25549459993229817
1105	22-23	0.20247268768015658
1105	24-25	0.1423741860491461
1105	26-27	0.21135397634650843
1105	28-29	0.13731720137007386
1105	30-31	0.06550687379307263
1105	32-33	0.10799412410664644
1105	34-35	0.0885129982303745
1105	36-37	0.03409474072048013
1105	38-39	0.015034200481501614
1105	40-41	-0.02822819410621591
1105	42-43	-0.05875157179529111
1105	44-45	0.04056631757717355
1105	46-47	0.053605062610234455
1105	48-49	-0.02985973649109752
1105	50-51	0.005713416973208041
1105	52-53	-0.013521858905047424
1105	54-55	-0.022314416791186176
1105	56-57	-0.0036149986698745806
1105	58-59	-0.019380940265794067
1105	60-61	-0.004466615086016645
1105	62-63	0.01853806972446037
1105	64-65	0.053886877649070186
1105	66-67	0.08721428781187512
1105	68-69	0.07056095880494695
1105	70-71	0.0420288263576758
1105	72-73	0.044534677549648904
1105	74-75	0.09569334458547729
1105	76-77	0.09129047092676501
1105	78-79	0.08583002002617945
1105	80-81	0.07348125098108937
1105	82-83	0.026140929354216524
1105	84-85	0.021237602258409538
1105	86-87	0.021242826086254496
1105	88-89	0.07770030989366106
1105	90-91	0.06503275163527178
1105	92-93	0.09661306859844387
1105	94-95	0.12718422461500367
1105	96-97	0.10226730407382689
1105	98-99	0.09297898573799657
1105	100	0.14711167892597743
1106	1	0.0013212619644882295
1106	2	0.0976011827386074
1106	3	0.11800697615295164
1106	4	0.08938249237547069
1106	5	0.15432072706619238
1106	6	0.015755700486351998
1106	7	0.08839022512511008
1106	8	0.22453001967264186
1106	9	0.1551843086131015
1106	10-11	0.15187640012874937
1106	12-13	0.18826999237635533
1106	14-15	0.26733033736025646
1106	16-17	0.20315945422554194
1106	18-19	0.23625875915502093
1106	20-21	0.15417227416767787
1106	22-23	0.1502234003888958
1106	24-25	0.1223818450951839
1106	26-27	0.1698326484574011
1106	28-29	0.04641514661934565
1106	30-31	0.06423387015140136
1106	32-33	0.038324637138501316
1106	34-35	0.018496115801536206
1106	36-37	0.0030772575865576357
1106	38-39	-0.05025532509449704
1106	40-41	-0.014733985650504877
1106	42-43	0.03957689416185417
1106	44-45	-0.01875872780565402
1106	46-47	-0.038458845083575
1106	48-49	-0.04090133407489205
1106	50-51	0.023719082369481725
1106	52-53	-0.06365277697987892
1106	54-55	-0.03215564950503946
1106	56-57	-0.04997251297780281
1106	58-59	-0.05315625456544737
1106	60-61	-0.05366858447958123
1106	62-63	-0.038107795012244594
1106	64-65	0.0789716214659677
1106	66-67	0.06394948630075703
1106	68-69	0.06982398839426196
1106	70-71	0.08372151031308306
1106	72-73	0.08416055644413234
1106	74-75	0.05732615784275907
1106	76-77	0.12582680046740435
1106	78-79	0.10343076880886315
1106	80-81	0.12424176511101237
1106	82-83	0.07972904622488741
1106	84-85	0.08921899690657398
1106	86-87	0.06231806067092549
1106	88-89	0.03320802303030135
1106	90-91	0.09412122876705098
1106	92-93	0.08436276750100546
1106	94-95	0.09231898726923404
1106	96-97	0.09919441193412126
1106	98-99	0.10187855685089531
1106	100	0.06868007483154415
1107	1	-0.0024402381375203674
1107	2	0.06031136359436928
1107	3	0.10597702016611521
1107	4	0.05043022114393381
1107	5	0.05805730757904115
1107	6	0.015186669381520801
1107	7	0.03247931317057606
1107	8	0.1769642625681982
1107	9	0.21403219219990177
1107	10-11	0.11413896929224876
1107	12-13	0.155797748983602
1107	14-15	0.250097673580008
1107	16-17	0.18037893661328752
1107	18-19	0.23467584127904928
1107	20-21	0.15958645935124594
1107	22-23	0.10311925191940219
1107	24-25	0.07760646558811146
1107	26-27	0.25020376882448403
1107	28-29	0.05689845155782791
1107	30-31	0.029422211725222525
1107	32-33	0.0441149079833032
1107	34-35	0.01685229920287057
1107	36-37	-0.00819285176095974
1107	38-39	-0.05239663912291803
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2216	42-43	0.9697999844183798
2216	44-45	0.9828884386191206
2216	46-47	0.9197611331733171
2216	48-49	0.8531029807177077
2216	50-51	0.8704342488902128
2216	52-53	0.8826340717939942
2216	54-55	0.8610329731974709
2216	56-57	0.8509634121075607
2216	58-59	0.8486396292197078
2216	60-61	0.8309728965317476
2216	62-63	0.6884384475214489
2216	64-65	0.5932785282247437
2216	66-67	0.6103431341712238
2216	68-69	0.5527488115477546
2216	70-71	0.46442850994365514
2216	72-73	0.4438079195338318
2216	74-75	0.45859032142156053
2216	76-77	0.4565226270067413
2216	78-79	0.3768252533300078
2216	80-81	0.4045773209518977
2216	82-83	0.42193099178968296
2216	84-85	0.37652780530464014
2216	86-87	0.31698383367839256
2216	88-89	0.19766837314132957
2216	90-91	0.16980268206934568
2216	92-93	0.12866815637175222
2216	94-95	0.0651143151344975
2216	96-97	0.020088915120489048
2216	98-99	-0.04429944471570835
2216	100	0.14712639977582853
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	524.0
6	5763.0
7	16687.0
8	29153.0
9	48063.0
10	73660.0
11	98681.0
12	112097.0
13	118901.0
14	128311.0
15	141437.0
16	159617.0
17	184326.0
18	219026.0
19	255416.0
20	291419.0
21	328735.0
22	369150.0
23	426867.0
24	566555.0
25	655605.0
26	757517.0
27	1191181.0
28	1093338.0
29	1712794.0
30	1659375.0
31	1972151.0
32	2420432.0
33	3464572.0
34	2522986.0
35	4092472.0
36	265409.0
37	47232.0
38	5150.0
39	236.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.41925037144094	32.0047391892896	25.40431134908843	26.171699090181022
2	13.73071749651146	35.26454944493974	26.77402806463418	24.230704993914618
3	12.065674750001946	38.008573923016804	23.5444252397837	26.38132608719755
4	10.657146705632645	37.98333215253818	23.38264941966605	27.97687172216312
5	10.77636455457717	37.35080312320559	24.294944340944372	27.577887981272873
6	10.727965206153039	34.18741111110499	25.86218149104288	29.222442191699088
7	14.594460622165453	38.89814609424348	21.411051029921868	25.096342253669203
8	12.593445636501299	36.72491065602059	19.362797693022824	31.318846014455286
9	10.04601876433909	35.76718109748824	20.22693516240439	33.95986497576828
10-11	15.13633149362595	48.60762846492225	18.664334537639355	17.591705503812445
12-13	15.405492719158302	49.73655224170128	17.369264135948363	17.488690903192055
14-15	16.94608718229899	48.35837981615927	16.929759186237526	17.76577381530422
16-17	16.758625825157473	48.09989777015324	17.979575025470968	17.161901379218317
18-19	17.1386498095801	46.57443517832743	19.565832176193585	16.721082835898883
20-21	16.398849593525984	46.60915643411265	19.96269211250271	17.02930185985866
22-23	17.149190525640833	47.84476127409338	20.267213014401232	14.738835185864554
24-25	17.40687193545036	47.9548680796329	19.466238282693947	15.172021702222787
26-27	19.338191651668676	48.18365848469275	18.28885551684502	14.189294346793549
28-29	18.880944886545787	49.33834265610928	18.760395680853847	13.020316776491084
30-31	19.33164947515695	49.359880041716	18.384981514726924	12.923488968400118
32-33	19.920398955102613	49.55108036448585	17.60094676428499	12.927573916126548
34-35	20.16387992502568	49.45243615037986	17.41183806056275	12.971845864031708
36-37	20.999053188535633	49.10992173649044	16.890617995582595	13.000407079391332
38-39	21.577894999308427	49.381140248482765	16.786215931673567	12.254748820535234
40-41	21.937564241382923	49.61807197346678	16.710918647131592	11.733445138018704
42-43	22.354262315545714	49.6058632209972	17.028178884659123	11.011695578797967
44-45	22.531587978911126	49.07733032580437	17.120909851426592	11.270171843857915
46-47	22.554406570768425	48.47734730082606	17.648184193509103	11.32006193489641
48-49	22.392373153253807	48.67483618383	18.22913231622307	10.703658346693128
50-51	22.733628630416845	48.664122583838015	18.507218691686155	10.095030094058984
52-53	23.068916040578276	48.86214049582894	18.536312425470094	9.532631038122688
54-55	23.07156035355916	49.3090618465901	18.35747292748631	9.261904872364433
56-57	22.733955579589082	49.71317097780725	17.88065967313573	9.672213769467932
58-59	22.275158369411148	49.566960054312915	18.07275462676465	10.085126949511292
60-61	22.173395061781786	48.86462042893759	18.721747907850183	10.240236601430432
62-63	22.244045745445675	47.795863689007966	19.309120427659103	10.650970137887256
64-65	21.795514034866343	47.48758655286544	19.570591358502625	11.146308053765594
66-67	20.940579442338844	47.14086212590624	20.203142938021923	11.715415493732996
68-69	20.588807866529525	46.79149668022076	19.948107605624642	12.671587847625071
70-71	20.10042182482016	46.5609115578947	20.205510911991357	13.133155705293778
72-73	20.296095512231073	46.9170640366881	20.133337752487428	12.653502698593403
74-75	20.052247679319795	47.61028593996235	19.844408935104106	12.493057445613749
76-77	19.015456480283504	48.6934241129978	19.90043632702893	12.390683079689763
78-79	18.071291774048905	49.873860046525905	19.507824475084583	12.547023704340601
80-81	17.058759674677404	51.762835061211376	18.859035905031455	12.319369359079763
82-83	16.075737567471002	53.56887264020429	18.792987684560806	11.562402107763901
84-85	14.972326365377295	55.579871479215704	18.7165515846988	10.731250570708204
86-87	14.054213107364752	57.35068273131528	18.354428326800843	10.240675834519118
88-89	13.44359162017309	58.7896237626207	18.01330399048354	9.753480626722665
90-91	13.25092888127963	60.15239623287905	17.280688074448662	9.315986811392657
92-93	13.270778826473236	61.225130702936426	16.422599585359116	9.081490885231217
94-95	13.532225430824418	61.51775819677924	15.736808848631009	9.213207523765334
96-97	13.920192769420709	61.238516477998076	15.406967069245342	9.43432368333588
98-99	14.468960082294915	60.47884481389716	15.160612537880816	9.891582565927107
100	15.531816442766214	59.22099414561585	14.944526208024293	10.302663203593648
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2690527.0
1	2679085.0
2	1698279.0
3	458571.0
4	181559.0
5	168630.0
6	142781.0
7	102004.5
8	66045.5
9	46157.0
10	41086.0
11	44415.5
12	53011.0
13	65530.5
14	82116.0
15	102660.5
16	125830.5
17	150654.5
18	175235.5
19	198165.5
20	219594.5
21	238223.5
22	253942.5
23	266342.5
24	274137.5
25	275926.0
26	271512.0
27	262636.5
28	249390.5
29	232552.0
30	214369.5
31	197105.5
32	181228.0
33	167300.0
34	157083.5
35	151110.5
36	148935.5
37	151742.5
38	159830.0
39	173211.5
40	195050.0
41	228805.0
42	280551.5
43	357027.0
44	463419.5
45	599855.5
46	751013.5
47	894277.0
48	1001784.0
49	1053070.0
50	1048665.5
51	1003836.0
52	937764.0
53	860746.5
54	772114.0
55	665081.0
56	541854.5
57	415725.0
58	299968.5
59	208068.0
60	140213.5
61	92223.0
62	63330.0
63	46236.5
64	36002.5
65	29441.5
66	24870.0
67	21381.0
68	18647.0
69	16412.0
70	14584.0
71	13169.5
72	11903.5
73	10965.5
74	10257.0
75	9616.0
76	9218.5
77	8641.0
78	8090.5
79	7402.0
80	6618.0
81	6038.5
82	5506.5
83	5100.5
84	4953.5
85	4825.0
86	4651.0
87	4510.0
88	4330.5
89	4185.0
90	4063.5
91	3942.0
92	3897.5
93	3819.0
94	3719.0
95	3636.0
96	3523.5
97	3369.5
98	3122.5
99	2781.5
100	2503.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.4034085212717265E-4
2	4.953834586430692E-4
3	1.7299104904996068E-4
4	1.572645900454188E-5
5	3.027343358374312E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.145291800908376E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.358968850681282E-5
22-23	1.179484425340641E-5
24-25	1.1008521303179316E-4
26-27	1.8282008592779937E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	4.1281954886922435E-5
42-43	6.683745076930298E-5
44-45	3.145291800908376E-5
46-47	2.732472252039152E-4
48-49	2.1623881131245085E-5
50-51	1.7692266380109617E-5
52-53	5.897422126703205E-6
54-55	1.572645900454188E-5
56-57	1.179484425340641E-5
58-59	2.358968850681282E-5
60-61	0.0
62-63	1.572645900454188E-5
64-65	1.0025617615395447E-4
66-67	1.6512781954768974E-4
68-69	3.6760597923116644E-4
70-71	9.101688148878612E-4
72-73	4.3247762262490174E-4
74-75	8.629894378742357E-4
76-77	0.0014330735767888788
78-79	7.725622985981198E-4
80-81	0.0010733308270599834
82-83	0.0011971766917207507
84-85	0.0017082866093683618
86-87	0.0022351229860205148
88-89	0.0023334133547989015
90-91	0.002427772108826153
92-93	0.002136832617242128
94-95	0.0010359804869241962
96-97	0.0020346106337126055
98-99	0.001824269244526858
100	0.0015687142857030523
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.5434842E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.34953476856604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.61442760717014	67.26430499327158
2	3.746063603916008	5.2706766349789795
3	0.33797625640778417	0.713294172033276
4	0.09160108757811986	0.25776375581661626
5	0.04011552036734822	0.14110540974209418
6	0.027141417516414485	0.11456316571435007
7	0.022228989156708626	0.1094659331884966
8	0.015243802336211911	0.08579155219651906
9	0.013462955349024749	0.08524013808724819
>10	0.0788565174801963	1.0382561639880403
>50	0.006432977845938873	0.3154729335605083
>100	0.004525475674008436	0.6458765076519623
>500	5.468938365801421E-4	0.27404882598048874
>1k	8.015661084149553E-4	1.2665422813261231
>5k	1.2973110694671343E-4	0.639225640284071
>10k+	4.4559814994732204E-4	21.77837189217955
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1154715	4.5398945273573945	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	330273	1.298506198701765	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	274051	1.0774629541634266	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	253369	0.9961492978804429	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	179415	0.7053906605749704	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	158432	0.6228935882518948	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	149814	0.5890109323266093	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	143895	0.5657397046146384	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	124897	0.49104688757256676	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	123412	0.4852084396671307	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	121518	0.4777619613284801	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	114070	0.4484792946620231	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	113159	0.4448975936237386	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105119	0.4132874110246094	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	101949	0.40082419226351	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	88923	0.3496109785152194	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	88732	0.3488600400977525	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	83522	0.32837632724433674	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82649	0.32494402756659546	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	71283	0.2802572943051897	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70353	0.27660089258663373	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68230	0.2682540744699731	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66886	0.26296998424444706	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56989	0.22405879305245932	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53533	0.21047113247253513	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50478	0.19846004940781625	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48093	0.18908314822635816	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	47883	0.18825750912861972	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46371	0.18231290762490288	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44320	0.17424916577032404	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42660	0.16772268528343914	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41693	0.16392081381909115	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41099	0.16158543465691666	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37446	0.1472232459710188	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36854	0.14489573003834663	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35354	0.13899830791164342	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35286	0.1387309581085662	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32946	0.1295309795909092	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32101	0.12620876512619972	No Hit
GAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31973	0.1257055184380544	No Hit
TAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31424	0.12354706193968101	No Hit
AAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30002	0.11795630576356636	No Hit
TAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29260	0.11503904761822384	No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27943	0.10986111099097844	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27875	0.10959376118790122	No Hit
GAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26975	0.1060553079118793	No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26704	0.1049898403143216	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0030548646616322603	0.0	0.0	3.93161475113547E-6	0.0
2	0.0071791285355733685	0.0	0.0	1.572645900454188E-5	0.0
3	0.011775186179650734	0.0	0.0	2.358968850681282E-5	0.0
4	0.01952046723938761	0.0	0.0	2.752130325794829E-5	0.0
5	0.03166915682039621	0.0	0.0	5.111099176476111E-5	0.0
6	0.05388671177906275	0.0	0.0	5.111099176476111E-5	0.0
7	0.08140801503701105	0.0	0.0	5.111099176476111E-5	0.0
8	0.1672980708903165	0.0	0.0	5.111099176476111E-5	0.0
9	0.4760359824527316	0.0	0.0	5.111099176476111E-5	0.0
10-11	1.1464175794762161	0.0	0.0	5.7008413891464315E-5	3.93161475113547E-6
12-13	2.0466138535478224	0.0	0.0	5.897422126703205E-5	3.93161475113547E-6
14-15	3.1449261607365204	0.0	0.0	6.290583601816752E-5	3.93161475113547E-6
16-17	4.359177855321452	0.0	0.0	6.6837450769303E-5	3.93161475113547E-6
18-19	5.697438576579324	0.0	0.0	8.256390977384487E-5	3.93161475113547E-6
20-21	7.513714848317123	0.0	0.0	8.256390977384487E-5	3.93161475113547E-6
22-23	9.611168412211878	0.0	0.0	8.256390977384487E-5	3.93161475113547E-6
24-25	11.817918900380825	0.0	0.0	8.256390977384487E-5	3.93161475113547E-6
26-27	14.089171460156898	0.0	0.0	8.256390977384487E-5	3.93161475113547E-6
28-29	16.589918270378877	0.0	0.0	8.256390977384487E-5	3.93161475113547E-6
30-31	19.42667463788452	0.0	0.0	8.256390977384487E-5	3.93161475113547E-6
32-33	22.644178406927004	0.0	0.0	8.45297171494126E-5	3.93161475113547E-6
34-35	25.55138734496562	0.0	0.0	8.649552452498034E-5	3.93161475113547E-6
36-37	27.805295586267057	0.0	0.0	8.649552452498034E-5	3.93161475113547E-6
38-39	29.45423053935228	0.0	0.0	8.649552452498034E-5	3.93161475113547E-6
40-41	30.801195855669164	0.0	0.0	9.632456140281902E-5	3.93161475113547E-6
42-43	31.84735529318405	0.0	0.0	1.1008521303179316E-4	3.93161475113547E-6
44-45	32.652736745917274	0.0	0.0	1.2188005728519957E-4	3.93161475113547E-6
46-47	33.319998606635735	0.0	0.0	1.277774794119028E-4	3.93161475113547E-6
48-49	33.92204873928448	0.0	0.0	1.2974328678747052E-4	3.93161475113547E-6
50-51	34.53691986763668	0.0	0.0	1.3760651628974145E-4	3.93161475113547E-6
52-53	35.214368542175336	1.965807375567735E-6	1.965807375567735E-6	1.5529878266985108E-4	3.93161475113547E-6
54-55	35.93375575126435	3.93161475113547E-6	3.93161475113547E-6	1.5923039742098653E-4	3.93161475113547E-6
56-57	36.714244185200755	3.93161475113547E-6	3.93161475113547E-6	1.6512781954768974E-4	3.93161475113547E-6
58-59	37.5403747347831	3.93161475113547E-6	3.93161475113547E-6	1.7299104904996068E-4	3.93161475113547E-6
60-61	38.39246573656719	3.93161475113547E-6	3.93161475113547E-6	1.7299104904996068E-4	7.86322950227094E-6
62-63	39.19685642238312	3.93161475113547E-6	3.93161475113547E-6	1.788884711766639E-4	7.86322950227094E-6
64-65	39.93332649756582	3.93161475113547E-6	3.93161475113547E-6	1.847858933033671E-4	7.86322950227094E-6
66-67	40.568948688574515	3.93161475113547E-6	3.93161475113547E-6	1.9264912280563803E-4	7.86322950227094E-6
68-69	41.090630718287926	1.179484425340641E-5	3.93161475113547E-6	2.024781596834767E-4	7.86322950227094E-6
70-71	41.5282115768598	1.572645900454188E-5	3.93161475113547E-6	2.0444396705904445E-4	7.86322950227094E-6
72-73	41.91907305734394	1.572645900454188E-5	3.93161475113547E-6	2.0444396705904445E-4	7.86322950227094E-6
74-75	42.27581008759559	1.572645900454188E-5	3.93161475113547E-6	2.083755818101799E-4	7.86322950227094E-6
76-77	42.611345885301745	1.572645900454188E-5	3.93161475113547E-6	2.1820461868801857E-4	7.86322950227094E-6
78-79	42.927058481432674	1.572645900454188E-5	3.93161475113547E-6	2.2999946294142499E-4	7.86322950227094E-6
80-81	43.22832239335318	1.572645900454188E-5	3.93161475113547E-6	2.6538399570164424E-4	7.86322950227094E-6
82-83	43.52737673778355	1.572645900454188E-5	3.93161475113547E-6	2.870078768328893E-4	7.86322950227094E-6
84-85	43.83072637132953	1.572645900454188E-5	7.86322950227094E-6	2.9487110633516023E-4	7.86322950227094E-6
86-87	44.13966872685901	1.572645900454188E-5	7.86322950227094E-6	2.9880272108629574E-4	1.179484425340641E-5
88	44.376760822811484	1.572645900454188E-5	7.86322950227094E-6	2.9880272108629574E-4	1.179484425340641E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTCAC	1335	0.0	48.58426	3
TTGCTGA	9210	0.0	45.264557	9
CAGCTCA	1515	0.0	42.501804	2
GCTTGCT	2180	0.0	40.962975	7
TACCCGA	16960	0.0	40.348732	6
TGAAAAA	20775	0.0	39.54583	1
ACCCGAC	18400	0.0	38.544796	7
GCCTGCT	2680	0.0	38.406406	7
GTAAAAA	13850	0.0	36.92151	1
CTTGCTG	6760	0.0	36.70976	8
GAAAACA	8485	0.0	36.448055	1
GACACGC	1275	0.0	34.651108	2
CCCGACG	22900	0.0	34.56217	8
CTGCTGA	11775	0.0	34.52626	9
CTACCCG	18615	0.0	34.38832	5
GTTGCTG	2775	0.0	33.196125	8
TCGCGAC	1190	0.0	33.1762	6
TGACGCC	1545	0.0	32.85436	3
CCTGCTG	9715	0.0	31.881367	8
GAAAATA	10930	0.0	31.648851	1
>>END_MODULE