##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1248461_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23458154
Sequences flagged as poor quality	0
Sequence length	100
%GC	29
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.03185450994993	31.0	28.0	33.0	23.0	34.0
2	29.589441394237586	31.0	30.0	33.0	24.0	34.0
3	29.91682286679506	31.0	30.0	34.0	25.0	34.0
4	33.29093666108595	35.0	33.0	37.0	28.0	37.0
5	33.10933379497807	35.0	33.0	37.0	27.0	37.0
6	32.941629592848614	35.0	32.0	37.0	26.0	37.0
7	33.04795897409489	35.0	32.0	37.0	27.0	37.0
8	32.93265612460384	35.0	32.0	37.0	26.0	37.0
9	34.118830919091074	37.0	32.0	39.0	26.0	39.0
10-11	34.357962097102785	37.0	33.5	39.0	27.0	39.0
12-13	34.28526690548625	37.0	33.0	39.0	27.0	39.0
14-15	34.954885431308874	37.0	32.0	40.0	27.0	41.0
16-17	34.73493952252168	37.0	32.0	40.0	27.0	41.0
18-19	34.21331797889979	36.5	32.0	39.0	26.0	41.0
20-21	33.84494378798946	35.5	32.0	39.0	25.0	40.5
22-23	33.3406592223753	35.0	32.0	38.5	25.0	40.0
24-25	32.90556616262303	35.0	31.0	38.0	24.5	40.0
26-27	32.45285824707264	35.0	30.0	37.5	23.0	40.0
28-29	32.28864973774151	35.0	30.0	37.0	23.0	40.0
30-31	32.16234529792924	35.0	30.0	37.0	23.0	40.0
32-33	31.97976955049404	35.0	30.0	37.0	22.0	40.0
34-35	31.852077533466613	35.0	30.0	37.0	22.0	40.0
36-37	31.63305963887866	34.5	29.0	37.0	21.5	39.5
38-39	31.417492910993765	34.0	28.0	37.0	21.0	39.0
40-41	31.167386444815733	34.0	27.5	37.0	20.0	39.0
42-43	30.94963124549357	33.0	27.0	37.0	20.0	39.0
44-45	30.70698203703497	33.0	26.0	36.0	19.5	39.0
46-47	30.59443908075631	33.0	26.0	36.0	20.0	39.0
48-49	30.40850550303319	33.0	26.5	35.0	19.5	39.0
50-51	30.209858861869524	33.0	26.5	35.0	18.5	39.0
52-53	29.954271700151683	33.0	26.0	35.0	18.0	39.0
54-55	29.760488016235207	33.0	26.0	35.0	18.0	38.0
56-57	29.549002044235877	32.5	26.0	35.0	17.0	38.0
58-59	29.27172005947271	32.0	26.0	35.0	15.0	37.0
60-61	29.014725988242724	31.5	26.0	35.0	15.0	37.0
62-63	28.967035108559692	31.0	26.0	35.0	16.5	36.5
64-65	29.2131621908527	32.0	26.0	35.0	18.5	36.0
66-67	29.661309069758858	32.0	26.5	35.0	20.0	36.0
68-69	29.664884990523976	32.0	27.0	35.0	20.0	35.5
70-71	29.97221910556133	33.0	28.0	35.0	20.0	35.0
72-73	29.969095884526975	33.0	28.0	35.0	20.0	35.0
74-75	29.870350262855297	33.0	28.0	35.0	20.0	35.0
76-77	29.737113755839438	33.0	28.0	35.0	20.0	35.0
78-79	29.59537638809942	33.0	27.0	35.0	19.5	35.0
80-81	29.48779025834684	33.0	27.0	35.0	19.0	35.0
82-83	29.375324034448745	33.0	27.0	35.0	18.0	35.0
84-85	29.2647090218608	33.0	27.0	35.0	17.5	35.0
86-87	29.090487554988343	33.0	27.0	35.0	16.0	35.0
88-89	28.91692400433555	33.0	27.0	35.0	14.5	35.0
90-91	28.675795333255977	33.0	26.5	35.0	10.5	35.0
92-93	28.426153311125844	33.0	26.0	35.0	10.0	35.0
94-95	28.102527824653208	33.0	25.0	35.0	9.0	35.0
96-97	27.744690886588945	32.0	25.0	35.0	8.0	35.0
98-99	27.316354880268925	32.0	24.5	35.0	7.5	35.0
100	25.98281889529756	30.0	20.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6046740327136852
1101	2	0.5150812001636034
1101	3	0.528496990663637
1101	4	0.4957765673178045
1101	5	0.4337212567265638
1101	6	0.34773632394572473
1101	7	0.36668063103631
1101	8	0.47574406606762665
1101	9	0.48025754489114547
1101	10-11	0.5534159782377941
1101	12-13	0.6846134546044311
1101	14-15	0.8010221838156681
1101	16-17	0.7718820446126671
1101	18-19	0.8140805514604637
1101	20-21	0.682886139468792
1101	22-23	0.603617686072532
1101	24-25	0.5823287794152492
1101	26-27	0.6882643247321028
1101	28-29	0.5016310452936921
1101	30-31	0.42152907452295807
1101	32-33	0.4651487303976154
1101	34-35	0.47786713027144145
1101	36-37	0.4197546373459353
1101	38-39	0.3419150261734707
1101	40-41	0.37720841788440396
1101	42-43	0.3666189021820614
1101	44-45	0.3748013591292185
1101	46-47	0.35447393279575934
1101	48-49	0.3269627071852703
1101	50-51	0.3735176405280498
1101	52-53	0.3277132382623691
1101	54-55	0.28050798495540263
1101	56-57	0.25088941728844816
1101	58-59	0.316455354943038
1101	60-61	0.2470973522826867
1101	62-63	0.2839741932086355
1101	64-65	0.36951130690574985
1101	66-67	0.4155150749443344
1101	68-69	0.4033661342793522
1101	70-71	0.3531389249950898
1101	72-73	0.4555231171140868
1101	74-75	0.48175450019639143
1101	76-77	0.4121529123013161
1101	78-79	0.4001606797106305
1101	80-81	0.35521738967586103
1101	82-83	0.3307483649307237
1101	84-85	0.3032506896687934
1101	86-87	0.2945328970370369
1101	88-89	0.29089370454974883
1101	90-91	0.29788601891615585
1101	92-93	0.25671255285521255
1101	94-95	0.28083932251188415
1101	96-97	0.2552071989401483
1101	98-99	0.2611783911077339
1101	100	0.240958910123112
1102	1	0.47283805026378545
1102	2	0.44594974273194765
1102	3	0.4040470975120307
1102	4	0.41808761423724405
1102	5	0.3508904317496686
1102	6	0.26200697378688176
1102	7	0.29734768534176226
1102	8	0.3501483573440538
1102	9	0.33774994436947026
1102	10-11	0.29882241875230875
1102	12-13	0.34454226817865674
1102	14-15	0.514442072109297
1102	16-17	0.517919354059245
1102	18-19	0.5705882293029134
1102	20-21	0.44441311281722307
1102	22-23	0.37280892720698233
1102	24-25	0.34726378975980765
1102	26-27	0.44879092296910983
1102	28-29	0.2614801447853665
1102	30-31	0.22137660906987833
1102	32-33	0.2671757121756002
1102	34-35	0.27216254104230586
1102	36-37	0.19896125064019898
1102	38-39	0.15829677550933496
1102	40-41	0.17853487727245465
1102	42-43	0.16751676947743377
1102	44-45	0.16308903637855465
1102	46-47	0.06434363117554298
1102	48-49	0.1771893968962175
1102	50-51	0.06906707350762886
1102	52-53	0.11171406037736631
1102	54-55	0.09149165250154567
1102	56-57	0.02573384702849779
1102	58-59	0.10961633724831543
1102	60-61	0.1201285274005528
1102	62-63	0.14856553324638
1102	64-65	0.21631174179963963
1102	66-67	0.21448768930673978
1102	68-69	0.23242020078406256
1102	70-71	0.28505493416946237
1102	72-73	0.2687464565152453
1102	74-75	0.2120308521922567
1102	76-77	0.2157860678975645
1102	78-79	0.23900247075292924
1102	80-81	0.17790411164271447
1102	82-83	0.11672922512910233
1102	84-85	0.10453455198341288
1102	86-87	0.13464002333949665
1102	88-89	0.14781148585223036
1102	90-91	0.16227790642676254
1102	92-93	0.1531023440599597
1102	94-95	0.08726197523241908
1102	96-97	0.10941910476004679
1102	98-99	0.16436433738955003
1102	100	0.06761898236929653
1103	1	0.2906138591113496
1103	2	0.289593129560604
1103	3	0.29873196664249946
1103	4	0.32501636210859886
1103	5	0.2999657661854229
1103	6	0.23570159302361304
1103	7	0.2918207025490318
1103	8	0.32337698706923845
1103	9	0.37173926467290386
1103	10-11	0.314362066426348
1103	12-13	0.3779786510373526
1103	14-15	0.4981423552376114
1103	16-17	0.45894364121486575
1103	18-19	0.5435847111741268
1103	20-21	0.4178241445672981
1103	22-23	0.29942334786144187
1103	24-25	0.3109949553161826
1103	26-27	0.35663393041038915
1103	28-29	0.1729664700130087
1103	30-31	0.1501756322200194
1103	32-33	0.16779692212267605
1103	34-35	0.17847133285040684
1103	36-37	0.07497428813482898
1103	38-39	0.06210595302671251
1103	40-41	0.15732616082503625
1103	42-43	0.0889485625955686
1103	44-45	0.0770230084777701
1103	46-47	0.10582207184028292
1103	48-49	0.044234720248084614
1103	50-51	0.03949712820857343
1103	52-53	0.06917164520417884
1103	54-55	0.05695292066017643
1103	56-57	0.09725393872447441
1103	58-59	0.03558204676230403
1103	60-61	0.07253705473934247
1103	62-63	0.1218034564115662
1103	64-65	0.22391355776586508
1103	66-67	0.20093171435511792
1103	68-69	0.20278447227775231
1103	70-71	0.2156135309316234
1103	72-73	0.15790270280540497
1103	74-75	0.13577435833446927
1103	76-77	0.16963957879382363
1103	78-79	0.17920559742758257
1103	80-81	0.18107362183617326
1103	82-83	0.12239343875570086
1103	84-85	0.06264325084518418
1103	86-87	0.07169885677381416
1103	88-89	0.11722508930636621
1103	90-91	0.1713662793970876
1103	92-93	0.12585581202900187
1103	94-95	0.12415252160668189
1103	96-97	0.13233927975285198
1103	98-99	0.1534784654401733
1103	100	0.12765310255230133
1104	1	0.26606537866106095
1104	2	0.24123037604314135
1104	3	0.22512387988973614
1104	4	0.263496336916333
1104	5	0.239860272366208
1104	6	0.19030957093050915
1104	7	0.25714821726280945
1104	8	0.37880931763805137
1104	9	0.23100923360954084
1104	10-11	0.2244884589697378
1104	12-13	0.3061825343654334
1104	14-15	0.41128169429304506
1104	16-17	0.4510739211408179
1104	18-19	0.4785702582104605
1104	20-21	0.38215631037832054
1104	22-23	0.27991293751124857
1104	24-25	0.2895616919163899
1104	26-27	0.3508573978120708
1104	28-29	0.2000872023040401
1104	30-31	0.1484117215075429
1104	32-33	0.17903896570422262
1104	34-35	0.1631444602478993
1104	36-37	0.09983260477107692
1104	38-39	0.10532198530458814
1104	40-41	0.1290283687684024
1104	42-43	0.1239481950158634
1104	44-45	0.10057569025276791
1104	46-47	0.1094764008804141
1104	48-49	0.08298282411622182
1104	50-51	0.0693871430907329
1104	52-53	0.10848175342471222
1104	54-55	0.03939447934035911
1104	56-57	0.05878849640313888
1104	58-59	0.07706622141053643
1104	60-61	0.06161000614715917
1104	62-63	0.11578006084495485
1104	64-65	0.20285675028779337
1104	66-67	0.22073712254114497
1104	68-69	0.20936518065959575
1104	70-71	0.1783555206813432
1104	72-73	0.1569984620721563
1104	74-75	0.15808564491851484
1104	76-77	0.21187462082308173
1104	78-79	0.19777888255003973
1104	80-81	0.18779104403269642
1104	82-83	0.16587811183107704
1104	84-85	0.08884418819175366
1104	86-87	0.1066111941933201
1104	88-89	0.13512516761723958
1104	90-91	0.15375357258461264
1104	92-93	0.10520186584887625
1104	94-95	0.08545599610506471
1104	96-97	0.1108169719811194
1104	98-99	0.1383141987262526
1104	100	0.09072317534965535
1105	1	0.20624260512484582
1105	2	0.2229502756211197
1105	3	0.19432034408055188
1105	4	0.2455334276051886
1105	5	0.15948086156129193
1105	6	0.13803377776066839
1105	7	0.16585026057509822
1105	8	0.2770231678985411
1105	9	0.2094093056471209
1105	10-11	0.2518060009148968
1105	12-13	0.25966572860050263
1105	14-15	0.3351861169051631
1105	16-17	0.2706655380007419
1105	18-19	0.34173679068213403
1105	20-21	0.2761657627336973
1105	22-23	0.2053664775991635
1105	24-25	0.1707724416933587
1105	26-27	0.2634671898659562
1105	28-29	0.10046440045177718
1105	30-31	0.051937973915514135
1105	32-33	0.05292745890517381
1105	34-35	0.0526946247004787
1105	36-37	-0.021657646168581124
1105	38-39	-0.03515301820548089
1105	40-41	-0.018587784237706018
1105	42-43	-0.013763386284256995
1105	44-45	-0.06062886472732387
1105	46-47	0.006496576222829731
1105	48-49	-0.0051486912899747495
1105	50-51	-0.026441097221972854
1105	52-53	-0.017757980624391934
1105	54-55	-0.004144782663001223
1105	56-57	-0.04790236487223609
1105	58-59	-0.10235690348699578
1105	60-61	-0.07702218223363388
1105	62-63	-0.009133193954845353
1105	64-65	0.08231454826675844
1105	66-67	0.05405036967281518
1105	68-69	0.049870658005374935
1105	70-71	0.04048749924527684
1105	72-73	0.04494842657350162
1105	74-75	0.04016380122543595
1105	76-77	0.036864958977268714
1105	78-79	0.02742255435478569
1105	80-81	0.05294827379642797
1105	82-83	0.03283578115926744
1105	84-85	0.020034581556135578
1105	86-87	0.04324570929717453
1105	88-89	0.04051798870326451
1105	90-91	0.03890362739355524
1105	92-93	0.057018676101172616
1105	94-95	0.07631767558963887
1105	96-97	0.11160128238861944
1105	98-99	0.09984881529887701
1105	100	0.1412114869539174
1106	1	0.047024736912366194
1106	2	0.15031249832407667
1106	3	0.15010139363994313
1106	4	0.13766903497480598
1106	5	0.15278413663541102
1106	6	0.0763867858402989
1106	7	0.13147867957358272
1106	8	0.1794269259307839
1106	9	0.16692532569983598
1106	10-11	0.15881544714915208
1106	12-13	0.22180847146449878
1106	14-15	0.3354479503248413
1106	16-17	0.2297424938770689
1106	18-19	0.26987746098056675
1106	20-21	0.17338073832128487
1106	22-23	0.1555171480843569
1106	24-25	0.07212546860836255
1106	26-27	0.1954883150125326
1106	28-29	0.03925006870614567
1106	30-31	0.01238778624936998
1106	32-33	-0.043967760009334
1106	34-35	-0.02372073666857233
1106	36-37	0.003280767127602502
1106	38-39	-0.009369829459689782
1106	40-41	-0.0026878549071440716
1106	42-43	0.003714154429427907
1106	44-45	-0.014333418752499938
1106	46-47	1.5011478894066954E-4
1106	48-49	-0.03758745029132626
1106	50-51	-0.06793312093341441
1106	52-53	-0.0678391661349167
1106	54-55	-0.059241584281700455
1106	56-57	-0.0951291876965179
1106	58-59	-0.06256427701390521
1106	60-61	0.005804855871478054
1106	62-63	0.005895340933566473
1106	64-65	0.05282229448771503
1106	66-67	0.06925456735574187
1106	68-69	0.08521700623031947
1106	70-71	0.09377347798642788
1106	72-73	0.04671295219590377
1106	74-75	0.09709261296765348
1106	76-77	0.09209462448370687
1106	78-79	0.07195186354204353
1106	80-81	0.10173510899227978
1106	82-83	0.034989419281814094
1106	84-85	0.07165432465405175
1106	86-87	0.09792242588529376
1106	88-89	0.0547787211340669
1106	90-91	0.08568448054682776
1106	92-93	0.05708546864924813
1106	94-95	0.06934571446982929
1106	96-97	0.07032764490809384
1106	98-99	-0.011720466479033576
1106	100	-0.007045613584963917
1107	1	0.06040188730225893
1107	2	0.10531943774722663
1107	3	0.14226919074303623
1107	4	0.13229124567522632
1107	5	0.11732559948367793
1107	6	0.030322802278377026
1107	7	0.03984001397384418
1107	8	0.22653895103805866
1107	9	0.1922956801914708
1107	10-11	0.12957711221127965
1107	12-13	0.17569196800054954
1107	14-15	0.22809817052435477
1107	16-17	0.2309691896677606
1107	18-19	0.180774122370849
1107	20-21	0.12869370804990865
1107	22-23	0.052789210798174224
1107	24-25	0.08088349589262123
1107	26-27	0.14795001974970745
1107	28-29	0.021264838841482003
1107	30-31	0.024006185533373525
1107	32-33	-0.00693341423272642
1107	34-35	-0.0025444247665973307
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2216	28-29	1.008395101473532
2216	30-31	1.0662596495700853
2216	32-33	1.1551178680298904
2216	34-35	1.130179897925558
2216	36-37	1.1489255669466196
2216	38-39	1.205986813612089
2216	40-41	1.2351404219049726
2216	42-43	1.1597940090312768
2216	44-45	1.174337387966009
2216	46-47	1.0869475654374803
2216	48-49	1.0466969444169223
2216	50-51	1.0769371655208708
2216	52-53	1.0269630238498557
2216	54-55	0.9586692706878743
2216	56-57	0.9955948534296191
2216	58-59	1.007924797839859
2216	60-61	0.983078011785544
2216	62-63	0.8410617802284399
2216	64-65	0.6781941271602285
2216	66-67	0.7371247996795702
2216	68-69	0.7273280389539458
2216	70-71	0.6482688861033203
2216	72-73	0.6313528329512543
2216	74-75	0.6599004020643768
2216	76-77	0.6956495972671703
2216	78-79	0.6654939611875399
2216	80-81	0.6664881919889858
2216	82-83	0.6294738615719417
2216	84-85	0.5882239866898438
2216	86-87	0.5361387331641438
2216	88-89	0.44444869431524126
2216	90-91	0.39617698141183766
2216	92-93	0.35971903582026243
2216	94-95	0.33004649493506477
2216	96-97	0.31595071606279745
2216	98-99	0.3011776073367116
2216	100	0.5775647813694604
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	341.0
6	4786.0
7	14805.0
8	25109.0
9	38985.0
10	56677.0
11	70825.0
12	77205.0
13	82953.0
14	92021.0
15	105822.0
16	124291.0
17	149023.0
18	177786.0
19	209736.0
20	241453.0
21	276069.0
22	312189.0
23	365148.0
24	480311.0
25	561380.0
26	649897.0
27	1007087.0
28	960127.0
29	1475217.0
30	1504116.0
31	1817745.0
32	2283461.0
33	3291080.0
34	2536663.0
35	4021525.0
36	367020.0
37	67524.0
38	8540.0
39	1232.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.957094300553297	32.46298530623753	25.779236798422577	25.800683594786587
2	13.190200486221748	35.50994348585501	27.372675640455775	23.927180387467466
3	11.66512235343106	38.16210156420117	24.977419321285417	25.195356761082348
4	10.371726702380768	38.254609869457724	24.749443135920192	26.62422029224131
5	10.336517239152302	37.66501826555748	25.726686556042406	26.27177793924781
6	10.306654990840284	34.73148398633584	27.14143661943732	27.820424403386557
7	14.146402994881866	39.50393112774347	22.54144124043179	23.808224636942874
8	12.590858598677457	36.344948541134144	20.299760160155824	30.76443270003258
9	10.356407413814402	35.744828855672104	21.091186459087957	32.80757727142554
10-11	13.887838318394532	50.39248825802747	19.82928835747263	15.890385066105372
12-13	13.4742870669684	50.735530192037984	19.907779158937387	15.882403582056238
14-15	14.467788045043953	49.710836155308726	19.537573161127682	16.28380263851964
16-17	14.308657876489345	49.66422123411757	20.087081447244316	15.940039442148773
18-19	14.694783741295245	48.763749696587375	20.891490438676463	15.649976123440915
20-21	14.335989539156696	48.76385590046819	21.04210746057649	15.858047099798632
22-23	14.885110837266492	49.28852133602593	21.26879770263238	14.5575701240752
24-25	15.026062928509532	49.16705734505024	20.88749624197413	14.919383484466097
26-27	16.274869999636586	49.12195282590465	20.245386376543802	14.357790797914962
28-29	15.976708994237143	49.877863364696125	20.50194998293557	13.643477658131156
30-31	16.393041839524116	49.871027788461106	20.228904201072258	13.50702617094252
32-33	16.78512938400865	49.85976944306871	19.843961293800017	13.511139879122627
34-35	16.83889959968717	49.903174393006374	19.799654738390753	13.458271268915704
36-37	17.40304458739592	49.62440778588119	19.496830398504503	13.475717228218384
38-39	17.750271398167136	49.79376893851068	19.542490427848673	12.913469235473515
40-41	17.989007103437853	49.95911974894353	19.548642127489302	12.503231020129316
42-43	18.173469074126547	50.014594080579535	19.73823501188777	12.073701833406151
44-45	18.199262981111833	49.79747444283811	19.784503045114317	12.21875953093574
46-47	18.058538593305332	49.49968234848718	20.15977894541062	12.282000112796872
48-49	17.948057033648688	49.56102040774567	20.490222332944217	12.000700225661424
50-51	18.255536497504153	49.40435631928721	20.727775146141095	11.61233203706754
52-53	18.480261031628086	49.38097322962781	20.850200336526914	11.288565402217186
54-55	18.42505926244618	49.498401461922455	20.805129474710338	11.271409800921022
56-57	18.1681549729886	49.5381019455816	20.542129223518348	11.751613857911444
58-59	17.838956367918495	49.22115774507728	20.69960583425327	12.240280052750958
60-61	17.850748187602488	48.70715530301319	21.168933412236957	12.273163097147371
62-63	17.866353104009626	47.978004489313854	21.557413488331058	12.598228918345459
64-65	17.537760426574614	47.841827586933796	21.699604403566212	12.920807582925379
66-67	16.859655335448338	47.70187397426213	22.20755991754324	13.230910772746299
68-69	16.548630578461665	47.780813689781944	21.856829288671538	13.813726443084857
70-71	16.043450270907165	48.00166945580715	21.983013984154237	13.971866289131441
72-73	16.000990363205858	48.52826600903344	21.908995843880614	13.561747783880088
74-75	15.700742786639795	49.21199094677827	21.664896300070584	13.422369966511349
76-77	14.910748987043942	50.17454940096972	21.66944349368392	13.245258118302422
78-79	14.281592628723402	51.142916361226796	21.32099697279884	13.25449403725096
80-81	13.64456140586462	52.509062254247	20.80015551279096	13.046220827097422
82-83	13.065352360900617	53.704919240393615	20.6514942252084	12.578234173497375
84-85	12.479434302096323	54.9527789323358	20.507346186228432	12.060440579339451
86-87	12.033640451312007	56.007760282231146	20.17416321121995	11.7844360552369
88-89	11.811368119931833	56.80042508991373	19.925019691357935	11.463187098796508
90-91	11.834939252508164	57.487532608843004	19.4764262694631	11.201101869185731
92-93	11.977524256059134	57.967590436618444	18.920909107496048	11.133976199826376
94-95	12.292306940387917	57.969547449114536	18.51321879638562	11.224926814111926
96-97	12.631679307185289	57.64672457984532	18.355482030303865	11.36611408266552
98-99	13.064414353618822	57.074721492564365	18.2203071551449	11.640556998671908
100	13.87232569196502	56.0577406309723	18.176331733112022	11.893601943950662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2367429.0
1	2343404.5
2	1455666.5
3	363593.5
4	129849.0
5	120046.5
6	101378.5
7	71819.5
8	46891.5
9	34465.5
10	32939.0
11	37750.5
12	47329.5
13	62276.0
14	82852.5
15	108565.0
16	137889.0
17	169335.5
18	201275.0
19	232584.5
20	261776.5
21	287238.0
22	308331.0
23	326212.0
24	341810.0
25	353610.0
26	360034.0
27	362036.0
28	359646.0
29	353564.0
30	347133.5
31	340696.5
32	335783.0
33	333963.0
34	336271.0
35	341373.5
36	349123.5
37	358913.5
38	367929.0
39	378394.5
40	391482.0
41	407454.5
42	430041.0
43	463830.5
44	512178.0
45	572895.0
46	636112.5
47	688878.0
48	716007.0
49	707167.5
50	667654.5
51	609509.0
52	544316.0
53	478466.5
54	412894.5
55	345385.0
56	274994.5
57	206869.0
58	147267.0
59	100848.0
60	67965.0
61	46624.0
62	33517.0
63	25542.5
64	20585.0
65	17211.0
66	14840.0
67	12972.5
68	11402.0
69	10146.0
70	8997.0
71	8092.5
72	7363.5
73	6747.5
74	6278.0
75	5962.0
76	5555.5
77	5104.0
78	4732.0
79	4374.5
80	4040.5
81	3691.0
82	3413.5
83	3273.5
84	3113.5
85	2914.0
86	2807.5
87	2650.0
88	2584.5
89	2581.5
90	2454.0
91	2319.5
92	2231.0
93	2144.5
94	2054.0
95	1936.5
96	1830.0
97	1725.0
98	1564.5
99	1349.0
100	1179.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.47605553275846E-4
2	5.66967034149405E-4
3	1.9609386143513253E-4
4	2.55774601871912E-5
5	2.941407921526988E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.1971825233988995E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.9840370218389733E-5
22-23	6.3943650467978E-6
24-25	1.1296711582676114E-4
26-27	1.982253164507318E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	2.3446005171591934E-5
42-43	5.541783040558093E-5
44-45	1.4920185109194867E-5
46-47	2.429858717783164E-4
48-49	8.525820062397067E-6
50-51	8.525820062397067E-6
52-53	2.1314550155992667E-6
54-55	6.3943650467978E-6
56-57	6.3943650467978E-6
58-59	8.525820062397067E-6
60-61	0.0
62-63	1.4920185109194867E-5
64-65	1.3001875595155526E-4
66-67	1.7477931127913985E-4
68-69	3.154553423086915E-4
70-71	8.973425615672912E-4
72-73	3.9858208791706287E-4
74-75	8.802909214424971E-4
76-77	0.0013854457601395235
78-79	7.758496256781331E-4
80-81	0.0011786946236263945
82-83	0.001255427004187968
84-85	0.0017222156526042075
86-87	0.002139980835661664
88-89	0.002252947951488425
90-91	0.0023872296174711786
92-93	0.002174084115911252
94-95	0.0010678589628152326
96-97	0.001933229699148535
98-99	0.0018415771334777664
100	0.0015218588811378765
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.3458154E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.21944221803767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.04792668275996	65.3377351120827
2	5.964225761728413	8.376092125020401
3	0.6811209802688165	1.4348380595243804
4	0.11235374445730323	0.315576690675991
5	0.04952885703955379	0.17389443575071983
6	0.02982992046578105	0.1256784225909343
7	0.019069494474706166	0.0937334485775673
8	0.014763704193718346	0.08293592588440048
9	0.010653126202144597	0.06732509218136601
>10	0.059914079985752565	0.818449502560991
>50	0.005021115736064676	0.24347881685619924
>100	0.0037897573126439116	0.5341408129739249
>500	5.044441772044839E-4	0.2543394896610382
>1k	7.570058347972625E-4	1.183056035541422
>5k	1.1687706702911926E-4	0.6234199033596145
>10k+	4.2444829605308847E-4	20.335306126758315
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1029993	4.390767491764271	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	304982	1.300110827134991	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	253060	1.0787720124951008	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	234097	0.9979344495734831	No Hit
AAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	172531	0.7354841305927141	No Hit
ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	141733	0.6041950274518617	No Hit
AAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	132232	0.5636931192454444	No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	125020	0.5329490121004407	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	118518	0.5052315710775879	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	116009	0.49453592980931066	No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	104710	0.4463693093667984	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	97346	0.41497723989705243	No Hit
AAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	96814	0.4127093717604548	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	91225	0.38888396759608623	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89766	0.38266438186056756	No Hit
AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	86596	0.3691509570616682	No Hit
AAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82344	0.35102506360901203	No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72815	0.3104037939217212	No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	68858	0.2935354589282686	No Hit
TAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64081	0.2731715377092332	No Hit
AAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61145	0.2606556338576343	No Hit
GAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	57757	0.24621289467193372	No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	55829	0.2379940041317829	No Hit
AAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49295	0.21014014998793168	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	44371	0.18914958099431012	No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	43142	0.18391046456596713	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42656	0.18183869029080466	No Hit
AACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40806	0.17395230673308734	No Hit
AAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39052	0.1664751625383651	No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39001	0.166257754126774	No Hit
TAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37442	0.15961187738813548	No Hit
AAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33025	0.14078260378033156	No Hit
TCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30682	0.1307946055772334	No Hit
AAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29761	0.12686846543849956	No Hit
AAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28071	0.11966414748577404	No Hit
CAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27446	0.11699982871627496	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27378	0.11670995083415345	No Hit
TAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26760	0.11407547243487276	No Hit
AAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	25798	0.10997455298485975	No Hit
GAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	24820	0.1058054269743476	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002476750728126348	0.0	0.0	0.0	0.0
2	0.006351735946485815	0.0	0.0	4.2629100311985335E-6	0.0
3	0.010448392486467605	0.0	0.0	2.1314550155992668E-5	0.0
4	0.017396935837321215	0.0	0.0	4.689201034318387E-5	0.0
5	0.02810110292566073	0.0	0.0	5.5417830405580936E-5	0.0
6	0.0473865079068029	0.0	0.0	5.5417830405580936E-5	0.0
7	0.07008224091290388	0.0	0.0	5.968074043677947E-5	0.0
8	0.14664410507322956	0.0	0.0	5.968074043677947E-5	0.0
9	0.4184131453821984	0.0	0.0	5.968074043677947E-5	0.0
10-11	1.0057611523907635	0.0	0.0	6.181219545237874E-5	0.0
12-13	1.7482748216249242	2.1314550155992667E-6	0.0	6.820656049917654E-5	0.0
14-15	2.632999169499868	4.2629100311985335E-6	0.0	7.67323805615736E-5	0.0
16-17	3.6032353611456385	4.2629100311985335E-6	0.0	8.099529059277213E-5	0.0
18-19	4.700182290558754	4.2629100311985335E-6	0.0	8.525820062397067E-5	0.0
20-21	6.151675447266652	4.2629100311985335E-6	0.0	8.738965563956994E-5	0.0
22-23	7.773708451227662	4.2629100311985335E-6	0.0	8.95211106551692E-5	0.0
24-25	9.385644752779779	4.2629100311985335E-6	0.0	8.95211106551692E-5	0.0
26-27	10.960781483487576	4.2629100311985335E-6	0.0	9.165256567076847E-5	0.0
28-29	12.625782489108051	4.2629100311985335E-6	0.0	9.804693071756627E-5	0.0
30-31	14.4308200892534	4.2629100311985335E-6	0.0	9.804693071756627E-5	0.0
32-33	16.413606117514618	4.2629100311985335E-6	0.0	9.804693071756627E-5	0.0
34-35	18.17738088001298	4.2629100311985335E-6	0.0	1.023098407487648E-4	0.0
36-37	19.51692149348154	4.2629100311985335E-6	4.2629100311985335E-6	1.0657275077996333E-4	0.0
38-39	20.486424464601946	4.2629100311985335E-6	4.2629100311985335E-6	1.470703960763494E-4	0.0
40-41	21.28088169256626	4.2629100311985335E-6	4.2629100311985335E-6	1.6412203620114354E-4	0.0
42-43	21.89738374127819	4.2629100311985335E-6	4.2629100311985335E-6	2.0035677146633106E-4	0.0
44-45	22.38174623629805	6.3943650467978E-6	4.2629100311985335E-6	2.046196814975296E-4	0.0
46-47	22.781276821697052	1.27887300935956E-5	4.2629100311985335E-6	2.3232859670032008E-4	0.0
48-49	23.140085959023033	1.7051640124794134E-5	4.2629100311985335E-6	2.685633319655076E-4	0.0
50-51	23.49647376345129	2.1314550155992668E-5	4.2629100311985335E-6	2.962722471682981E-4	2.1314550155992667E-6
52-53	23.874131357480216	2.55774601871912E-5	4.2629100311985335E-6	3.410328024958827E-4	4.2629100311985335E-6
54-55	24.254881692736777	2.55774601871912E-5	4.2629100311985335E-6	3.9858208791706287E-4	4.2629100311985335E-6
56-57	24.65705741381014	2.55774601871912E-5	4.2629100311985335E-6	4.220280930886548E-4	4.2629100311985335E-6
58-59	25.07564533850362	2.55774601871912E-5	4.2629100311985335E-6	4.348168231822504E-4	4.2629100311985335E-6
60-61	25.503490172329844	2.55774601871912E-5	4.2629100311985335E-6	4.5613137333824305E-4	4.2629100311985335E-6
62-63	25.907739799133385	2.55774601871912E-5	4.2629100311985335E-6	4.7531446847863643E-4	4.2629100311985335E-6
64-65	26.283123130660663	2.55774601871912E-5	4.2629100311985335E-6	4.8384028854103354E-4	4.2629100311985335E-6
66-67	26.62090546425776	2.7708915202790465E-5	4.2629100311985335E-6	5.008919286658277E-4	6.3943650467978E-6
68-69	26.9117723415065	2.9840370218389733E-5	4.2629100311985335E-6	5.264693888530189E-4	8.525820062397067E-6
70-71	27.17326563718526	2.9840370218389733E-5	4.2629100311985335E-6	5.435210289778129E-4	8.525820062397067E-6
72-73	27.420906606717647	3.1971825233989E-5	4.2629100311985335E-6	5.669670341494049E-4	8.525820062397067E-6
74-75	27.6567648929238	3.410328024958827E-5	4.2629100311985335E-6	5.75492854211802E-4	8.525820062397067E-6
76-77	27.88918940509982	3.410328024958827E-5	4.2629100311985335E-6	6.095961344613903E-4	8.525820062397067E-6
78-79	28.117248697403895	3.410328024958827E-5	4.2629100311985335E-6	6.522252347733756E-4	8.525820062397067E-6
80-81	28.338071699930012	4.689201034318387E-5	4.2629100311985335E-6	7.119059752101551E-4	8.525820062397067E-6
82-83	28.559806965202803	4.689201034318387E-5	4.2629100311985335E-6	7.587979855533389E-4	8.525820062397067E-6
84-85	28.784500690037248	4.689201034318387E-5	4.2629100311985335E-6	7.886383557717286E-4	8.525820062397067E-6
86-87	29.013414695802577	4.689201034318387E-5	4.2629100311985335E-6	8.078214509121221E-4	8.525820062397067E-6
88	29.186286354842757	4.689201034318387E-5	4.2629100311985335E-6	8.22741636021317E-4	8.525820062397067E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGCTG	6250	0.0	36.170734	8
CTGCTGA	7260	0.0	35.799812	9
TACCCGA	12745	0.0	34.516617	6
GCCTGCT	1965	0.0	33.963936	7
CCCGACG	13795	0.0	33.524757	8
GCTTGCT	2075	0.0	32.84295	7
CTACCCG	13220	0.0	32.17439	5
TGAAAAA	23560	0.0	31.519655	1
CTTGCTG	5070	0.0	30.869423	8
TTGCTGA	8210	0.0	30.397902	9
ACCCGAC	14610	0.0	30.335678	7
GACACGC	1720	0.0	27.598717	2
CCGACGC	16715	0.0	27.387022	9
GCTGAAC	13800	0.0	26.3435	10-11
TGAACCG	11840	0.0	25.921118	12-13
ACTGGCC	1175	0.0	25.199781	3
ATGTGCC	1025	0.0	24.76076	3
CGCTTGC	1330	0.0	24.383142	6
CTCACGA	6580	0.0	24.285452	5
GCCCTAC	15320	0.0	24.266655	7
>>END_MODULE